pycom04g18980

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000126
Physical Location & Seq
Reverse (-)
20809303 .. 20815100
5798 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18980.1

Sequence Viewer

Length: 3207 bp
ATGGAGAAGATACAGGCGGAACTGAAGGAGGCGGAGACGAAGCAGAGGAGTCTGAGCAAGGCGTACGAGTGCGTACACGCGCAGGCGACTGCGCTTATTATCTTCGCCGTGCAATGGAAGGATCTGGAGGAGCACTTCGAGTCGACTCGGTACTCGCTCGAGTTGCAACTCCGGGAACTCGCCCGTCGCGAGACGGCGGTCCAGGTGCGGTACGGGGAGTTGGAGGCGAAGGAGATGAAGTTCAACTCTGAAATCGAATCGAAAGCGGACGAGTTGGGGGGGCTTCAGAGGCTGATCGACGAGAAGGTTGAGGATGTTATACAGAATAAGAACCACTTGCACTCTCTCCAGTCATTGATTCAGGAATGTAGCAATGAGGTTGTGGTGCAAGAGACGAGATTGATGGAGGCTGAGGGTTTTGTTGTGGAGAAGAAGAGGGAGTGCGATTCGATTGAGAGGCGGGTTGAGCGGAGGCAGAAAAAATTGGATTGGGTTGAGAGAAGGGTGGAGGAGAAGTTGAAGGTGGCGGAGGAGAAGTCGAAATTGGCTGAGGTGAAGGCGGAGGAAGCGAGGGGGTTTGAAGAGGCGTTGGAGAAGTGTGTCGAAGAGATTGAGTTGAAGAAGAGGGAGTTGAATGAGATTAGGGGGTTGATTGAGAAGCGCAAGGAGGAGTTCAATTCGATAGGGGAGCAGATTATGGGGGCGGAAGAGTTGATGAAGGTCCAGGAGGAGCAGCTGGGTTTTAAAGAAGAGAGAATTAAGGAGGCGCAAAGGGCGGTTGAACAGTGCGACAAAGAGTTGAAATGGAGAGAGAAGCTGATTACGGAGGCGGAAAGGGCGGTTGAAGAATGTGACAAGGAGTTGAAGCTGAAAAAGGAGAAACTTGTTGTCATTGATAAGTCATTAGTGGAGTGCTCGAATGCGCTGGAATCAAGAGAGAAAAAGATTAGGGAAATAGACTCGAAAAAGGATAAAGATTTGTGCTTGCGTGAGAAATCATTGGAGGAGTGGTCCTGTAAGCTTGAGTTTAAAGAGATGGAAGTTAGATTGGGAGAGAGAATTGTCGAGTTAAAAGCTGGGGAACTCCAAAAGTTGAAGGAAGGCGAAAGGTGTTTGGAATCTGTCTCGCAGGTACTTAAACAGAAAGAGAACCGACTTCAAGACCTTGACGAAGAGCTCGAATTGAAGCAGAAAGAACTTGATTTGATAAAAAAATCCACTGAAGAACGCGCCAAAAACTTGGAATTGAAAGAGAGGCAACTTGAAGATCAGACCAAAGAGCTTGAACTGAAGCAGAAAGGATTTGATTCGATAAAAAAATTCTCTGAGGAACGCACCCAAAACCTGAAATCGAAAGAGAATACTAGTATTCTTCTTTCTCATGTGAAAATTGAGCAATTTGAACATATCCCTGCCAACAATGTTGTTGTTCCTTCTACCTCAAGTAACCAGTCCAGCGTCAATATGGATGGTAGAGGCTTGCTGTTGTTCATAAATGAGCATTTGAAGACATATGTTCTATTGGGTAGTGAAATCTCAGCTGCTCTTCAGGCGTCACCAGACCCGGCAAAATTAGTTTTGGATGCTATGCAAGGTTTTTACCATGCAAATTCAAGTGCGGACAAAAGCGAGTGTGATTTTGATTTACGCATTACTAGGATGACTTGTAGCCTTTTGTTGGAGAATTTAAAGAGTGTCTCCCCCCAAATTAACCCTGAGGTGAGAGAGGAAGCAATTAAGTTGGCAGGTGATTGGAAGGTTAAAATGAGGACGGACAATTGCTTGGAGGTTTTGGGTTTTCTGCGGCTTGTTAGTACATATGGAATCACCTCGTTTTATGATGCGAAGGAGCTTCAAAGTCTTTGTGCGATTGTTGCTAAGGATGAACACACAACTGAATTATCCTGGGCTTTTGAAACTCGTTCTTCTCCGAATCTTCAGCTAACTGCCACCAGAGATGCTAGTGCTACTCATTTGCAGGGGTTTTTAAATGAGCTTTTCAGTAGGGATCGTTTGACACAGACCGAAACTTGGGCTGCTTTTCAATCGTCATCAGAACCAGAACCAGAAAAGTTTGTGATGGATGTGATGCAAACTTCTTTTGATAAATACTCCAAAATAGAGGATGCTGGTTTTAAAGAAATTGTCATGGCAAATTACATTTCACTACTCAACATGCTGATGAGAATTAAACCGCATGTTGGACATCATGTTAACAAAGATGCTCTAAAGCTAGCAATCAATTGGAAATTGAAAATTGGTGCCAGTACCCAACCCCTCGAGCTTTTGGGTTTTTTGCAGTTCATAGCTACATATGGACTGCTTTCCGTGTTTAATAGAGAGACTTTAGTGTTGCTTGGAAGGATTTCTCAGCAGAAGCAGGCGCTAGAAATATGTCAGACGCTTGGTTTAGCTGATAAGATCCCTGAAATCATTCGGGGTCTTATTGAAAAGAAGCAACTAATTGAGGCCGTTCGATTGATTTGCCCGTTTAAGTTAATTGAGAAGTTCCCGCCAGCACCACTCTTGGAAGAATTTGTGGAGAATGCGAAGTTGTTGTGCATTCAAATGAGCAAGAGACATAAATTATTTTGTGAAAGGGATAAGTGTGTAAACGACCAGATTGCTGCTCTTAGAGTTGTGATTCAATGTATCAAAGATTGCAACCTCGAGTCCGAGTACCCTTCCAGGTTCATTGAAACACGAATAGCTCTTCTGGAAAAAATGAAGCAGAATCGCAGACGCCAAATGACATCTCCTCCCTCCAAGGTTGAACAACAAGGGAAGAAAAATTCTCAAGTCTCCAAGGTTGAGGGACAAGAGCAGAAACAGTCTCCTGCCTCCGAAGTTGAGGGACGGGAGCAGAAAAAGTCTCCTTCCCCTAGAGTTGAGGGACGAGAGCACATCAAACCTCTTGCCTCCAATGTCGAACAAGGGGCAGAAAAGCCTTTTGCTTCCACTGTTGAGGGACAAGAGCAGAAAAGATCTGTTCCCTCCCAGGCTGAACCAGAAGAACACAGAAGAGGCAAGAAACGTCAACCCAACACTTTGCCTCGCCGCATTCAACCACCACAACAACGTCAAAATATGTATAATCAGACATCTGCTTCCCCTGGTACATCCTATTCATCACCACTAATCACCCCAGGCTATCGGAAGCCAGGTCCGTTGCCTGGGGAGAATTTTCAAAGCCATGCCGAATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida

Protein Analysis

1069

Amino Acids

122.86

Weight (kDa)

6.09

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 480 - 635 9.2e-34 Frigida-like protein
Frigida PF07899 656 - 917 1.2e-51 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1736
Acc36I ACCTGC 2 cut(s) 1120, 1736
AccB1I GGYRCC 1 cut(s) 2261
AccBSI CCGCTC 1 cut(s) 469
AccI GTMKAC 1 cut(s) 143
AccII CGCG 3 cut(s) 80, 189, 1230
AclWI GGATC 3 cut(s) 129, 2016, 2416
AcsI RAATTY 6 cut(s) 1319, 1609, 1684, 2534, 2791, 3181
AcuI CTGAAG 6 cut(s) 44, 269, 1242, 1310, 1532, 1922
AcyI GRCGYC 2 cut(s) 1553, 2743
AfaI GTAC 9 cut(s) 65, 75, 152, 212, 1134, 1816, 2269, 2681, 3118
AfiI CCNNNNNNNGG 5 cut(s) 114, 1678, 2031, 2201, 3173
AhlI ACTAGT 1 cut(s) 1364
AjnI CCWGG 9 cut(s) 201, 723, 1904, 2687, 2997, 3112, 3145, 3160, 3172
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
AloI GAACNNNNNNTCC 2 cut(s) 224, 256
Alw21I GWGCWC 4 cut(s) 135, 917, 1179, 2904
AlwI GGATC 3 cut(s) 129, 2016, 2416
AlwNI CAGNNNCTG 1 cut(s) 292
Ama87I CYCGRG 3 cut(s) 158, 2279, 2669
AoxI GGCC 1 cut(s) 2469
ApeKI GCWGC 4 cut(s) 733, 1541, 2036, 2627
ApoI RAATTY 6 cut(s) 1319, 1609, 1684, 2534, 2791, 3181
Asp700I GAANNNNTTC 2 cut(s) 2365, 2433
AspLEI GCGC 7 cut(s) 82, 94, 663, 769, 925, 1232, 2386
AspS9I GGNCC 4 cut(s) 199, 721, 1011, 3164
AsuC2I CCSGG 2 cut(s) 173, 1565
AsuHPI GGTGA 7 cut(s) 565, 1548, 1732, 1760, 1819, 3123, 3133
AsuNHI GCTAGC 1 cut(s) 2233
AvaI CYCGRG 3 cut(s) 158, 2279, 2669
AvaII GGWCC 4 cut(s) 199, 721, 1011, 3164
AxyI CCTNAGG 1 cut(s) 1716
BanI GGYRCC 1 cut(s) 2261
BanII GRGCYC 1 cut(s) 1179
BbsI GAAGAC 1 cut(s) 1514
Bbv12I GWGCWC 4 cut(s) 135, 917, 1179, 2904
BbvCI CCTCAGC 2 cut(s) 411, 549
BbvI GCAGC 4 cut(s) 745, 1528, 2023, 2614
BccI CCATC 4 cut(s) 397, 1030, 1463, 2074
BceAI ACGGC 3 cut(s) 92, 210, 2456
BcgI CGANNNNNNTGC 4 cut(s) 2466, 2500, 2606, 2640
BciT130I CCWGG 9 cut(s) 203, 725, 1906, 2689, 2999, 3114, 3147, 3162, 3174
BcnI CCSGG 2 cut(s) 173, 1565
BcuI ACTAGT 1 cut(s) 1364
BfaI CTAG 6 cut(s) 1365, 1656, 1962, 2234, 2387, 2883
BfoI RGCGCY 1 cut(s) 2387
BfuAI ACCTGC 2 cut(s) 1120, 1736
BglII AGATCT 1 cut(s) 2984
BisI GCNGC 6 cut(s) 734, 1542, 1805, 2037, 2628, 3058
BlsI GCNGC 6 cut(s) 735, 1543, 1806, 2038, 2629, 3059
Bme18I GGWCC 4 cut(s) 199, 721, 1011, 3164
BmeT110I CYCGRG 3 cut(s) 158, 2279, 2669
BmgT120I GGNCC 4 cut(s) 199, 721, 1011, 3164
BmiI GGNNCC 1 cut(s) 2263
BmsI GCATC 6 cut(s) 1573, 1831, 1948, 2079, 2116, 2212
BmtI GCTAGC 1 cut(s) 2237
BoxI GACNNNNGTC 1 cut(s) 197
BpiI GAAGAC 1 cut(s) 1514
BpmI CTGGAG 2 cut(s) 146, 332
Bpu10I CCTNAGC 3 cut(s) 411, 549, 1878
BpuEI CTTGAG 3 cut(s) 1043, 1426, 2781
BpuMI CCSGG 2 cut(s) 173, 1565
BsaHI GRCGYC 2 cut(s) 1553, 2743
BsaJI CCNNGG 7 cut(s) 1905, 2766, 2805, 2997, 3112, 3145, 3173
Bsc4I CCNNNNNNNGG 5 cut(s) 114, 1678, 2031, 2201, 3173
Bse1I ACTGG 3 cut(s) 349, 1450, 2265
Bse21I CCTNAGG 1 cut(s) 1716
Bse3DI GCAATG 2 cut(s) 119, 379
BseBI CCWGG 9 cut(s) 203, 725, 1906, 2689, 2999, 3114, 3147, 3162, 3174
BseDI CCNNGG 7 cut(s) 1905, 2766, 2805, 2997, 3112, 3145, 3173
BseGI GGATG 8 cut(s) 319, 1474, 1588, 1665, 1888, 2089, 2131, 3119
BseLI CCNNNNNNNGG 5 cut(s) 114, 1678, 2031, 2201, 3173
BseMI GCAATG 2 cut(s) 119, 379
BseMII CTCAG 7 cut(s) 44, 402, 540, 1317, 1551, 1707, 2384
BseNI ACTGG 3 cut(s) 349, 1450, 2265
BseRI GAGGAG 8 cut(s) 61, 143, 524, 545, 683, 743, 1019, 2748
BseXI GCAGC 4 cut(s) 745, 1528, 2023, 2614
BseYI CCCAGC 2 cut(s) 736, 1076
Bsh1236I CGCG 3 cut(s) 80, 189, 1230
BshFI GGCC 1 cut(s) 2471
BshNI GGYRCC 1 cut(s) 2261
BsiHKAI GWGCWC 4 cut(s) 135, 917, 1179, 2904
BsiHKCI CYCGRG 3 cut(s) 158, 2279, 2669
BsiSI CCGG 2 cut(s) 172, 1565
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 4 cut(s) 2829, 2868, 2907, 2982
BslI CCNNNNNNNGG 5 cut(s) 114, 1678, 2031, 2201, 3173
BsmBI CGTCTC 3 cut(s) 29, 185, 386
BsmFI GGGAC 4 cut(s) 2829, 2868, 2907, 2982
BsmI GAATGC 4 cut(s) 925, 2551, 2562, 3060
BsnI GGCC 1 cut(s) 2471
BsoBI CYCGRG 3 cut(s) 158, 2279, 2669
Bsp1286I GDGCHC 4 cut(s) 135, 917, 1179, 2904
Bsp143I GATC 6 cut(s) 121, 294, 1267, 2008, 2421, 2984
Bsp68I TCGCGA 1 cut(s) 189
BspANI GGCC 1 cut(s) 2471
BspCNI CTCAG 7 cut(s) 45, 403, 541, 1318, 1550, 1708, 2383
BspFNI CGCG 3 cut(s) 80, 189, 1230
BspLI GGNNCC 1 cut(s) 2263
BspMI ACCTGC 2 cut(s) 1120, 1736
BspOI GCTAGC 1 cut(s) 2237
BspPI GGATC 3 cut(s) 129, 2016, 2416
BspQI GCTCTTC 3 cut(s) 1167, 1551, 2718
BspT107I GGYRCC 1 cut(s) 2261
BsrBI CCGCTC 1 cut(s) 469
BsrDI GCAATG 2 cut(s) 119, 379
BsrI ACTGG 3 cut(s) 349, 1450, 2265
BssECI CCNNGG 7 cut(s) 1905, 2766, 2805, 2997, 3112, 3145, 3173
BssMI GATC 6 cut(s) 121, 294, 1267, 2008, 2421, 2984
BssNI GRCGYC 2 cut(s) 1553, 2743
BssT1I CCWWGG 2 cut(s) 2766, 2805
Bst2UI CCWGG 9 cut(s) 203, 725, 1906, 2689, 2999, 3114, 3147, 3162, 3174
Bst4CI ACNGT 3 cut(s) 786, 2832, 2962
BstACI GRCGYC 2 cut(s) 1553, 2743
BstC8I GCNNGC 6 cut(s) 84, 986, 1481, 2235, 2382, 2517
BstDEI CTNAG 9 cut(s) 53, 411, 549, 1326, 1537, 1716, 1878, 2370, 2633
BstF5I GGATG 8 cut(s) 319, 1474, 1588, 1665, 1888, 2089, 2131, 3119
BstFNI CGCG 3 cut(s) 80, 189, 1230
BstH2I RGCGCY 1 cut(s) 2387
BstHHI GCGC 7 cut(s) 82, 94, 663, 769, 925, 1232, 2386
BstKTI GATC 6 cut(s) 124, 297, 1270, 2011, 2424, 2987
BstMBI GATC 6 cut(s) 121, 294, 1267, 2008, 2421, 2984
BstMWI GCNNNNNNNGC 8 cut(s) 163, 289, 466, 566, 773, 836, 1550, 1874
BstNI CCWGG 9 cut(s) 203, 725, 1906, 2689, 2999, 3114, 3147, 3162, 3174
BstNSI RCATGY 2 cut(s) 2179, 2201
BstPAI GACNNNNGTC 1 cut(s) 197
BstUI CGCG 3 cut(s) 80, 189, 1230
BstV1I GCAGC 4 cut(s) 745, 1528, 2023, 2614
BstV2I GAAGAC 1 cut(s) 1514
BstX2I RGATCY 3 cut(s) 121, 2421, 2984
BstXI CCANNNNNNTGG 1 cut(s) 1240
BstYI RGATCY 3 cut(s) 121, 2421, 2984
Bsu36I CCTNAGG 1 cut(s) 1716
BsuRI GGCC 1 cut(s) 2471
BtsCI GGATG 8 cut(s) 319, 1474, 1588, 1665, 1888, 2089, 2131, 3119
BtsIMutI CAGTG 3 cut(s) 791, 1218, 2958
BtuMI TCGCGA 1 cut(s) 189
BveI ACCTGC 2 cut(s) 1120, 1736
Cac8I GCNNGC 6 cut(s) 84, 986, 1481, 2235, 2382, 2517
CaiI CAGNNNCTG 1 cut(s) 292
CfoI GCGC 7 cut(s) 82, 94, 663, 769, 925, 1232, 2386
Cfr13I GGNCC 4 cut(s) 199, 721, 1011, 3164
CseI GACGC 4 cut(s) 1447, 1542, 2410, 2751
Csp6I GTAC 9 cut(s) 64, 74, 151, 211, 1133, 1815, 2268, 2680, 3117
CviAII CATG 7 cut(s) 1382, 1604, 2149, 2176, 2198, 2210, 3194
CviQI GTAC 9 cut(s) 64, 74, 151, 211, 1133, 1815, 2268, 2680, 3117
DdeI CTNAG 9 cut(s) 53, 411, 549, 1326, 1537, 1716, 1878, 2370, 2633
DpnI GATC 6 cut(s) 123, 296, 1269, 2010, 2423, 2986
DpnII GATC 6 cut(s) 121, 294, 1267, 2008, 2421, 2984
DraI TTTAAA 5 cut(s) 745, 1030, 1689, 1989, 2137
EciI GGCGGA 6 cut(s) 32, 47, 542, 575, 719, 845
Ecl136II GAGCTC 1 cut(s) 1177
Eco130I CCWWGG 2 cut(s) 2766, 2805
Eco24I GRGCYC 1 cut(s) 1179
Eco47I GGWCC 4 cut(s) 199, 721, 1011, 3164
Eco53kI GAGCTC 1 cut(s) 1177
Eco57I CTGAAG 6 cut(s) 44, 269, 1242, 1310, 1532, 1922
Eco81I CCTNAGG 1 cut(s) 1716
Eco88I CYCGRG 3 cut(s) 158, 2279, 2669
EcoICRI GAGCTC 1 cut(s) 1177
EcoRII CCWGG 9 cut(s) 201, 723, 1904, 2687, 2997, 3112, 3145, 3160, 3172
EcoT14I CCWWGG 2 cut(s) 2766, 2805
EcoT38I GRGCYC 1 cut(s) 1179
ErhI CCWWGG 2 cut(s) 2766, 2805
Esp3I CGTCTC 3 cut(s) 29, 185, 386
FaeI CATG 7 cut(s) 1385, 1607, 2152, 2179, 2201, 2213, 3197
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 4 cut(s) 2829, 2868, 2907, 2982
FatI CATG 7 cut(s) 1381, 1603, 2148, 2175, 2197, 2209, 3193
FauI CCCGC 2 cut(s) 453, 2520
FauNDI CATATG 3 cut(s) 1513, 1819, 2314
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 6 cut(s) 734, 1542, 1805, 2037, 2628, 3058
FokI GGATG 8 cut(s) 326, 1481, 1595, 1672, 1895, 2096, 2138, 3106
FriOI GRGCYC 1 cut(s) 1179
Fsp4HI GCNGC 6 cut(s) 734, 1542, 1805, 2037, 2628, 3058
FspBI CTAG 6 cut(s) 1365, 1656, 1962, 2234, 2387, 2883
GlaI GCGC 7 cut(s) 81, 93, 662, 768, 924, 1231, 2385
GluI GCNGC 6 cut(s) 734, 1542, 1805, 2037, 2628, 3058
GsaI CCCAGC 2 cut(s) 740, 1080
GsuI CTGGAG 2 cut(s) 146, 332
HaeII RGCGCY 1 cut(s) 2387
HaeIII GGCC 1 cut(s) 2471
HapII CCGG 2 cut(s) 172, 1565
HgaI GACGC 4 cut(s) 1447, 1542, 2410, 2751
HhaI GCGC 7 cut(s) 82, 94, 663, 769, 925, 1232, 2386
Hin1I GRCGYC 2 cut(s) 1553, 2743
Hin1II CATG 7 cut(s) 1385, 1607, 2152, 2179, 2201, 2213, 3197
Hin6I GCGC 7 cut(s) 80, 92, 661, 767, 923, 1230, 2384
HinP1I GCGC 7 cut(s) 80, 92, 661, 767, 923, 1230, 2384
HincII GTYRAC 3 cut(s) 144, 2215, 3038
HindII GTYRAC 3 cut(s) 144, 2215, 3038
HindIII AAGCTT 1 cut(s) 1019
HpaI GTTAAC 1 cut(s) 2215
HpaII CCGG 2 cut(s) 172, 1565
HphI GGTGA 7 cut(s) 565, 1548, 1732, 1760, 1819, 3123, 3133
Hpy166II GTNNAC 5 cut(s) 76, 144, 2215, 2614, 3038
Hpy188III TCNNGA 7 cut(s) 125, 188, 362, 933, 1160, 2717, 3204
Hpy8I GTNNAC 5 cut(s) 76, 144, 2215, 2614, 3038
Hpy99I CGWCG 2 cut(s) 189, 302
HpyCH4III ACNGT 3 cut(s) 786, 2832, 2962
HpyCH4IV ACGT 2 cut(s) 3034, 3079
HpyF10VI GCNNNNNNNGC 8 cut(s) 163, 289, 466, 566, 773, 836, 1550, 1874
HpyF3I CTNAG 9 cut(s) 53, 411, 549, 1326, 1537, 1716, 1878, 2370, 2633
HpySE526I ACGT 2 cut(s) 3034, 3079
Hsp92I GRCGYC 2 cut(s) 1553, 2743
Hsp92II CATG 7 cut(s) 1385, 1607, 2152, 2179, 2201, 2213, 3197
HspAI GCGC 7 cut(s) 80, 92, 661, 767, 923, 1230, 2384
KspAI GTTAAC 1 cut(s) 2215
Kzo9I GATC 6 cut(s) 121, 294, 1267, 2008, 2421, 2984
LguI GCTCTTC 3 cut(s) 1167, 1551, 2718
LmnI GCTCC 5 cut(s) 130, 688, 730, 1849, 2860
Lsp1109I GCAGC 4 cut(s) 745, 1528, 2023, 2614
LweI GCATC 6 cut(s) 1573, 1831, 1948, 2079, 2116, 2212
MaeI CTAG 6 cut(s) 1365, 1656, 1962, 2234, 2387, 2883
MaeII ACGT 2 cut(s) 3034, 3079
MaeIII GTNAC 3 cut(s) 851, 1445, 1554
MalI GATC 6 cut(s) 123, 296, 1269, 2010, 2423, 2986
MbiI CCGCTC 1 cut(s) 469
MboI GATC 6 cut(s) 121, 294, 1267, 2008, 2421, 2984
MfeI CAATTG 2 cut(s) 1777, 2242
MflI RGATCY 3 cut(s) 121, 2421, 2984
MhlI GDGCHC 4 cut(s) 135, 917, 1179, 2904
MlyI GAGTC 5 cut(s) 58, 139, 149, 953, 2681
MmeI TCCRAC 4 cut(s) 201, 570, 1659, 2182
MroXI GAANNNNTTC 2 cut(s) 2365, 2433
MslI CAYNNNNRTG 2 cut(s) 2180, 2567
MspA1I CMGCKG 2 cut(s) 736, 1541
MspI CCGG 2 cut(s) 172, 1565
MunI CAATTG 2 cut(s) 1777, 2242
Mva1269I GAATGC 4 cut(s) 925, 2551, 2562, 3060
MvaI CCWGG 9 cut(s) 203, 725, 1906, 2689, 2999, 3114, 3147, 3162, 3174
MvnI CGCG 3 cut(s) 80, 189, 1230
MwoI GCNNNNNNNGC 8 cut(s) 163, 289, 466, 566, 773, 836, 1550, 1874
NciI CCSGG 2 cut(s) 173, 1565
NdeI CATATG 3 cut(s) 1513, 1819, 2314
NdeII GATC 6 cut(s) 121, 294, 1267, 2008, 2421, 2984
NheI GCTAGC 1 cut(s) 2233
NlaIII CATG 7 cut(s) 1385, 1607, 2152, 2179, 2201, 2213, 3197
NlaIV GGNNCC 1 cut(s) 2263
NmuCI GTSAC 2 cut(s) 851, 1554
NruI TCGCGA 1 cut(s) 189
NspI RCATGY 2 cut(s) 2179, 2201
PaeR7I CTCGAG 3 cut(s) 158, 2279, 2669
PaqCI CACCTGC 1 cut(s) 1736
PciSI GCTCTTC 3 cut(s) 1167, 1551, 2718
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 1176
PctI GAATGC 4 cut(s) 925, 2551, 2562, 3060
PdmI GAANNNNTTC 2 cut(s) 2365, 2433
Pfl23II CGTACG 1 cut(s) 63
PfoI TCCNGGA 2 cut(s) 171, 723
PkrI GCNGC 6 cut(s) 735, 1543, 1806, 2038, 2629, 3059
PleI GAGTC 5 cut(s) 57, 139, 148, 953, 2680
PpsI GAGTC 5 cut(s) 57, 139, 148, 953, 2680
PshAI GACNNNNGTC 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 1179
Psp6I CCWGG 9 cut(s) 201, 723, 1904, 2687, 2997, 3112, 3145, 3160, 3172
PspFI CCCAGC 2 cut(s) 736, 1076
PspGI CCWGG 9 cut(s) 201, 723, 1904, 2687, 2997, 3112, 3145, 3160, 3172
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 2263
PspPI GGNCC 4 cut(s) 199, 721, 1011, 3164
PspXI VCTCGAGB 3 cut(s) 158, 2279, 2669
PstNI CAGNNNCTG 1 cut(s) 292
PsuI RGATCY 3 cut(s) 121, 2421, 2984
PvuII CAGCTG 2 cut(s) 736, 1541
RruI TCGCGA 1 cut(s) 189
RsaI GTAC 9 cut(s) 65, 75, 152, 212, 1134, 1816, 2269, 2681, 3118
RsaNI GTAC 9 cut(s) 64, 74, 151, 211, 1133, 1815, 2268, 2680, 3117
RseI CAYNNNNRTG 2 cut(s) 2180, 2567
SacI GAGCTC 1 cut(s) 1179
SalI GTCGAC 1 cut(s) 142
SapI GCTCTTC 3 cut(s) 1167, 1551, 2718
SatI GCNGC 6 cut(s) 734, 1542, 1805, 2037, 2628, 3058
Sau3AI GATC 6 cut(s) 121, 294, 1267, 2008, 2421, 2984
Sau96I GGNCC 4 cut(s) 199, 721, 1011, 3164
SchI GAGTC 5 cut(s) 58, 139, 149, 953, 2681
SduI GDGCHC 4 cut(s) 135, 917, 1179, 2904
SfaNI GCATC 6 cut(s) 1573, 1831, 1948, 2079, 2116, 2212
Sfr274I CTCGAG 3 cut(s) 158, 2279, 2669
SinI GGWCC 4 cut(s) 199, 721, 1011, 3164
SlaI CTCGAG 3 cut(s) 158, 2279, 2669
SmiMI CAYNNNNRTG 2 cut(s) 2180, 2567
SmlI CTYRAG 6 cut(s) 158, 1022, 1441, 2279, 2669, 2796
SmoI CTYRAG 6 cut(s) 158, 1022, 1441, 2279, 2669, 2796
SpeI ACTAGT 1 cut(s) 1364
SspMI CTAG 6 cut(s) 1365, 1656, 1962, 2234, 2387, 2883
SstI GAGCTC 1 cut(s) 1179
StyI CCWWGG 2 cut(s) 2766, 2805
TaaI ACNGT 3 cut(s) 786, 2832, 2962
TaiI ACGT 2 cut(s) 3037, 3082
TaqII GACCGA 1 cut(s) 2039
TatI WGTACW 1 cut(s) 1814
TauI GCSGC 2 cut(s) 1807, 3060
TscAI CASTG 3 cut(s) 791, 1225, 2965
TseFI GTSAC 2 cut(s) 851, 1554
TseI GCWGC 4 cut(s) 733, 1541, 2036, 2627
Tsp45I GTSAC 2 cut(s) 851, 1554
TspDTI ATGAA 8 cut(s) 251, 731, 1480, 1899, 2293, 2683, 2741, 3117
TspGWI ACGGA 4 cut(s) 839, 1787, 2317, 3156
TspRI CASTG 3 cut(s) 791, 1225, 2965
VpaK11BI GGWCC 4 cut(s) 199, 721, 1011, 3164
XapI RAATTY 6 cut(s) 1319, 1609, 1684, 2534, 2791, 3181
XceI RCATGY 2 cut(s) 2179, 2201
XcmI CCANNNNNNNNNTGG 1 cut(s) 1462
XhoI CTCGAG 3 cut(s) 158, 2279, 2669
XmiI GTMKAC 1 cut(s) 143
XmnI GAANNNNTTC 2 cut(s) 2365, 2433
XspI CTAG 6 cut(s) 1365, 1656, 1962, 2234, 2387, 2883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.