Rw3G003750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000126
Physical Location & Seq
Forward (+)
3237871 .. 3241907
4037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003750.1

Sequence Viewer

Length: 2361 bp
ATGGAGGAGGAGGAGGAGATAGCATCGGAGTTGAGAGAATCGGAGTTGAAGCAGAGAAATGTGAGGGAAGCGTGGAAGGAGCTGGACGACCACTTTGACTCGACCCGGAGAGGGCTCCGGACCCGGTTCCAGGAGCTCCAGGCCCGCGAGGACGAAATCGGAGTCCGTGAGAAGCTGTTGGAGGCGAAAGAGCTGAGATTCGAAGCTCAGGCCAAGGGATTGAATGAAGTTGTGAAGCGGGCCGAAGAAGGTAAGAGTCAGTTGGAGTCTCTTAGGGTTTTGATTGAAAAACATGAAGCAGAGCTTGGTGTTAAAGAAAAGAGGCTCTCGGAGGTTGGGGAATTGGTTGGGGAAAAGGAGAGGGAGTATGATTTGATTCAGAATCGTGTGAAAGAGAGGGGTAAGAGACTGAACAAGTATAATCGTGCGATTGAGTTGAAAAGGGGGCAACTTGGTTCGACTCTGAAATCGATGATGGAATGCCAGAGCAGAATCGATTCGAGGGAGAAGATAATGAGGGAAATGGAGTTGAAAGGGAGGGACTTTTGTCTGCTGAAGAAATCGATGGAGGATTGGTTATGTAAAGTTGAATTGGGAAACAGGGAGCTTGATGGATGGGTTGAGGTTCTTGAGGTGAAGGAGAAGGATGTTGAGTCCAAAGTTGAGGAACTCCATTTGATTGTTGAGAGGGTCAGTGAATGCCTTGATGAGGTTCAGGTGAAAAAGGAGCATTTTGTTTCGTTAGAAAAACCGATACAGGAGCAGGAAATGCATTTGTGTTCACTCCAAAAGTTGGTGCAACAGCGTGACAAGGAGTTGGATTCACTGTCCCATGAACTTCAAAGGAAAGAGAGGCATCTTGAAGAGGTTGAATTGAAGATGGCGAAATTAGGACATGCGATTCCTTCATACGTAAGTGATGATCATTCCAGCATTCATTGGGATGGTAGAGGCTTGCAAATGTTCATGAATGAGCAGTTGAAGAGAATATATTCAATGGGTAGCGAAATGTCAGCAATTCTTAAGGCCTCATCTGACCCAGCAAAATTGGTTTTGGATGCAATGCAAGGATTTTACCCTTCAAGTTCAACTCTGGACAACAAGGAGACTCATTTTGATTTGACAGTTATTAGAAGGAGTTGTATTCTTTTGTTAGAGGATTTGAAGAGAATGTCACCACAAATTAATGCTCGGGTGAGAAACGAAGCAATAAAGTTGGCGGCTGACTGGAAGACTAAGATGACAGTATCTAGTGAGAATACACAGGAGATTTTGGGGTTTTTGCGGCTTGTTAATACGTATGGATTGAGTTCGATGTATGATGCGAAAGATCTTCAAAGCCTTCTTGCTGTTGTTCATAAGCCCGAACAATCATCTCAATCAAGTAGGGCCCTTGGTATCAAAGATAAAGCACCTGGGACCAGCATCATTTCTTCCCTTATCAAAACTGTGGAACCAGAATCTTCACTGGCTATAAATGCAACAACTCTCTCTTCTCCAAATCCTTCTTCCCAGAATGACATGTTGGCTTCTCTTTTATTGGTCTCTGACCCAGCAGAACATGTCTTGAATAGTGTGCAGAGATCATTTTCTCAGTACTGGAAACATGAAGATGTTAGTTTCAAAGAACGTATCATGTTGAATAACATGTCTTTGTTGGAGTGTCTAACGATTATCTCACCGCACATTGGACCTCATGTGAAAGAAGATGCAAAACTTCTTGCTGCCTATTGGAAACCAAAGATGACAGGCAATACTGAAAATTCAGTTGAGACTTTAGGATTTTTGCTGTTTATATCTACATATGGATTGGTTTCTACATTGAATACAGAGGAGATTATAAAGCTTCTTGAGGTGATATCGCAGAATAAAGGGGCTGTAGATTTATGTCAGACGCATGGTTATGCAGATAAGATCTCTGATTTTATTCGGAATCTGATAGAAAGGAAGCAACTGATGGATGCTGTTGGATTTATTTGCACCTTCAACCTAGCTGACAAGCTCCCCCCAATACCACTCTTAAAACAATATGTAGAGGATGCAAAGAAGTGTTCCGAAGTAACTTGCAGCTTAAAGATACTTCATGATGAAAAGGTTAAGATTGTAGACAACCTCATAGCTGATCTAAGAGCTGGGAAACAATGCATCAAAGAATACCATCTCGAGTGCGAATACCCATATGCAGACATTGAAATGCAAATAGTTCAGCTGGAAAAACTGAAGGAGAATTGGAGATGCTCGGCGCCATCTCTTGGCTCCAGTATTGTTGAATCACAAGTGCAGAGAAAAAGAAAGAAGCCTAATACTAGTAGTTCTGCCAGTACGCGTGCATTTCCATCACAACAGCAGCGTAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida TEX13

Protein Analysis

786

Amino Acids

90.03

Weight (kDa)

6.06

Isoelectric Point (pI)

50.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 315 - 483 6.2e-33 Frigida-like protein
Frigida PF07899 505 - 743 9.4e-51 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1841
AccB1I GGYRCC 1 cut(s) 2242
AccB7I CCANNNNNTGG 2 cut(s) 793, 2252
AccI GTMKAC 1 cut(s) 2106
AccII CGCG 2 cut(s) 147, 2326
AccIII TCCGGA 1 cut(s) 117
AciI CCGC 5 cut(s) 145, 238, 1220, 1285, 1682
AcsI RAATTY 1 cut(s) 1762
AcuI CTGAAG 2 cut(s) 575, 2240
AcyI GRCGYC 1 cut(s) 2243
AfaI GTAC 2 cut(s) 1598, 2323
AfiI CCNNNNNNNGG 6 cut(s) 111, 130, 709, 793, 1688, 2252
AflII CTTAAG 1 cut(s) 1022
AflIII ACRYGT 4 cut(s) 1521, 1561, 1647, 2324
AhlI ACTAGT 1 cut(s) 2306
AjnI CCWGG 3 cut(s) 129, 138, 1414
AjuI GAANNNNNNNTTGG 6 cut(s) 288, 320, 1492, 1508, 1524, 1540
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 5 cut(s) 273, 400, 1100, 1549, 1766
Ama87I CYCGRG 2 cut(s) 1191, 2162
Aor13HI TCCGGA 1 cut(s) 117
AoxI GGCC 5 cut(s) 141, 210, 240, 1026, 1389
ApaI GGGCCC 1 cut(s) 1393
ApeKI GCWGC 3 cut(s) 1724, 2067, 2347
ApoI RAATTY 1 cut(s) 1762
ArsI GACNNNNNNTTYG 4 cut(s) 77, 109, 147, 179
AseI ATTAAT 1 cut(s) 1185
Asp700I GAANNNNTTC 2 cut(s) 496, 991
AspLEI GCGC 1 cut(s) 2245
AspS9I GGNCC 7 cut(s) 120, 142, 240, 1389, 1390, 1419, 1691
AsuC2I CCSGG 2 cut(s) 106, 124
AsuHPI GGTGA 6 cut(s) 646, 730, 1167, 1207, 1671, 1867
AsuII TTCGAA 1 cut(s) 201
AvaI CYCGRG 2 cut(s) 1191, 2162
AvaII GGWCC 3 cut(s) 120, 1419, 1691
BaeGI GKGCMC 1 cut(s) 1393
BanI GGYRCC 1 cut(s) 2242
BanII GRGCYC 3 cut(s) 117, 138, 1393
BbsI GAAGAC 1 cut(s) 1238
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 1711, 2079
BciT130I CCWGG 3 cut(s) 131, 140, 1416
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 2 cut(s) 106, 124
BcoDI GTCTC 5 cut(s) 273, 400, 1100, 1549, 1766
BcuI ACTAGT 1 cut(s) 2306
BfaI CTAG 3 cut(s) 1251, 1991, 2307
BfmI CTRYAG 1 cut(s) 1878
BfoI RGCGCY 1 cut(s) 2246
BfrI CTTAAG 1 cut(s) 1022
BglII AGATCT 2 cut(s) 1330, 1914
BisI GCNGC 5 cut(s) 1221, 1286, 1725, 2068, 2348
BlsI GCNGC 5 cut(s) 1222, 1287, 1726, 2069, 2349
BmcAI AGTACT 1 cut(s) 1598
Bme1390I CCNGG 5 cut(s) 106, 124, 131, 140, 1416
Bme18I GGWCC 3 cut(s) 120, 1419, 1691
BmeT110I CYCGRG 2 cut(s) 1191, 2162
BmgT120I GGNCC 7 cut(s) 120, 142, 240, 1389, 1390, 1419, 1691
BmiI GGNNCC 8 cut(s) 116, 122, 128, 1391, 1420, 1455, 2244, 2257
BmrFI CCNGG 5 cut(s) 106, 124, 131, 140, 1416
BoxI GACNNNNGTC 1 cut(s) 546
BpiI GAAGAC 1 cut(s) 1238
BpmI CTGGAG 2 cut(s) 122, 2242
Bpu10I CCTNAGC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 650, 1871
BpuMI CCSGG 2 cut(s) 106, 124
Bsa29I ATCGAT 3 cut(s) 470, 495, 563
BsaAI YACGTR 2 cut(s) 913, 1299
BsaHI GRCGYC 1 cut(s) 2243
BsaI GGTCTC 1 cut(s) 1549
BsaJI CCNNGG 3 cut(s) 213, 1393, 1415
BsaWI WCCGGW 1 cut(s) 117
BsaXI ACNNNNNCTCC 4 cut(s) 350, 380, 560, 590
Bsc4I CCNNNNNNNGG 6 cut(s) 111, 130, 709, 793, 1688, 2252
Bse1I ACTGG 5 cut(s) 1232, 1473, 1604, 2259, 2319
Bse3DI GCAATG 1 cut(s) 1068
BseAI TCCGGA 1 cut(s) 117
BseBI CCWGG 3 cut(s) 131, 140, 1416
BseCI ATCGAT 3 cut(s) 470, 495, 563
BseDI CCNNGG 3 cut(s) 213, 1393, 1415
BseGI GGATG 6 cut(s) 620, 652, 949, 1063, 1966, 2044
BseLI CCNNNNNNNGG 6 cut(s) 111, 130, 709, 793, 1688, 2252
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 3 cut(s) 185, 221, 1607
BseNI ACTGG 5 cut(s) 1232, 1473, 1604, 2259, 2319
BseRI GAGGAG 5 cut(s) 20, 23, 26, 29, 1847
BseSI GKGCMC 1 cut(s) 1393
BseXI GCAGC 2 cut(s) 1711, 2079
BseYI CCCAGC 3 cut(s) 1039, 1552, 2132
BsgI GTGCAG 2 cut(s) 1598, 2300
Bsh1236I CGCG 2 cut(s) 147, 2326
BshFI GGCC 5 cut(s) 143, 212, 242, 1028, 1391
BshNI GGYRCC 1 cut(s) 2242
BshVI ATCGAT 3 cut(s) 470, 495, 563
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 2 cut(s) 1191, 2162
BsiSI CCGG 3 cut(s) 106, 118, 124
BslFI GGGAC 3 cut(s) 554, 814, 1432
BslI CCNNNNNNNGG 6 cut(s) 111, 130, 709, 793, 1688, 2252
BsmAI GTCTC 5 cut(s) 273, 400, 1100, 1549, 1766
BsmFI GGGAC 3 cut(s) 554, 814, 1432
BsmI GAATGC 3 cut(s) 485, 704, 933
BsnI GGCC 5 cut(s) 143, 212, 242, 1028, 1391
Bso31I GGTCTC 1 cut(s) 1549
BsoBI CYCGRG 2 cut(s) 1191, 2162
Bsp119I TTCGAA 1 cut(s) 201
Bsp120I GGGCCC 1 cut(s) 1389
Bsp1286I GDGCHC 3 cut(s) 117, 138, 1393
Bsp13I TCCGGA 1 cut(s) 117
Bsp143I GATC 5 cut(s) 922, 1330, 1583, 1914, 2122
BspACI CCGC 5 cut(s) 145, 238, 1220, 1285, 1682
BspANI GGCC 5 cut(s) 143, 212, 242, 1028, 1391
BspCNI CTCAG 3 cut(s) 186, 220, 1606
BspDI ATCGAT 3 cut(s) 470, 495, 563
BspEI TCCGGA 1 cut(s) 117
BspFNI CGCG 2 cut(s) 147, 2326
BspHI TCATGA 2 cut(s) 966, 2083
BspLI GGNNCC 8 cut(s) 116, 122, 128, 1391, 1420, 1455, 2244, 2257
BspT104I TTCGAA 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 2242
BspTI CTTAAG 1 cut(s) 1022
BspTNI GGTCTC 1 cut(s) 1549
BsrDI GCAATG 1 cut(s) 1068
BsrI ACTGG 5 cut(s) 1232, 1473, 1604, 2259, 2319
BssECI CCNNGG 3 cut(s) 213, 1393, 1415
BssMI GATC 5 cut(s) 922, 1330, 1583, 1914, 2122
BssNI GRCGYC 1 cut(s) 2243
BssT1I CCWWGG 2 cut(s) 213, 1393
Bst2UI CCWGG 3 cut(s) 131, 140, 1416
Bst4CI ACNGT 4 cut(s) 828, 1126, 1246, 1450
Bst6I CTCTTC 4 cut(s) 858, 977, 1160, 1498
BstACI GRCGYC 1 cut(s) 2243
BstAFI CTTAAG 1 cut(s) 1022
BstAPI GCANNNNNTGC 1 cut(s) 769
BstBAI YACGTR 2 cut(s) 913, 1299
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 4 cut(s) 145, 240, 956, 2328
BstDEI CTNAG 6 cut(s) 194, 207, 272, 1236, 1593, 2126
BstENI CCTNNNNNAGG 1 cut(s) 707
BstF5I GGATG 6 cut(s) 620, 652, 949, 1063, 1966, 2044
BstFNI CGCG 2 cut(s) 147, 2326
BstH2I RGCGCY 1 cut(s) 2246
BstHHI GCGC 1 cut(s) 2245
BstKTI GATC 5 cut(s) 925, 1333, 1586, 1917, 2125
BstMAI GTCTC 5 cut(s) 273, 400, 1100, 1549, 1766
BstMBI GATC 5 cut(s) 922, 1330, 1583, 1914, 2122
BstMWI GCNNNNNNNGC 3 cut(s) 769, 1478, 2353
BstNI CCWGG 3 cut(s) 131, 140, 1416
BstNSI RCATGY 4 cut(s) 899, 1525, 1565, 1651
BstPAI GACNNNNGTC 1 cut(s) 546
BstSCI CCNGG 5 cut(s) 104, 122, 129, 138, 1414
BstSFI CTRYAG 1 cut(s) 1878
BstSLI GKGCMC 1 cut(s) 1393
BstSNI TACGTA 2 cut(s) 913, 1299
BstUI CGCG 2 cut(s) 147, 2326
BstV1I GCAGC 2 cut(s) 1711, 2079
BstV2I GAAGAC 1 cut(s) 1238
BstX2I RGATCY 2 cut(s) 1330, 1914
BstYI RGATCY 2 cut(s) 1330, 1914
Bsu15I ATCGAT 3 cut(s) 470, 495, 563
BsuRI GGCC 5 cut(s) 143, 212, 242, 1028, 1391
BsuTUI ATCGAT 3 cut(s) 470, 495, 563
BtsCI GGATG 6 cut(s) 620, 652, 949, 1063, 1966, 2044
BtsIMutI CAGTG 3 cut(s) 700, 824, 1466
Cac8I GCNNGC 4 cut(s) 145, 240, 956, 2328
CciI TCATGA 2 cut(s) 966, 2083
CfoI GCGC 1 cut(s) 2245
Cfr13I GGNCC 7 cut(s) 120, 142, 240, 1389, 1390, 1419, 1691
ClaI ATCGAT 3 cut(s) 470, 495, 563
CseI GACGC 1 cut(s) 1903
Csp6I GTAC 2 cut(s) 1597, 2322
CviQI GTAC 2 cut(s) 1597, 2322
DdeI CTNAG 6 cut(s) 194, 207, 272, 1236, 1593, 2126
DinI GGCGCC 1 cut(s) 2244
DpnI GATC 5 cut(s) 924, 1332, 1585, 1916, 2124
DpnII GATC 5 cut(s) 922, 1330, 1583, 1914, 2122
Eam1104I CTCTTC 4 cut(s) 858, 977, 1160, 1498
EarI CTCTTC 4 cut(s) 858, 977, 1160, 1498
Ecl136II GAGCTC 1 cut(s) 136
Eco105I TACGTA 2 cut(s) 913, 1299
Eco130I CCWWGG 2 cut(s) 213, 1393
Eco147I AGGCCT 1 cut(s) 1028
Eco24I GRGCYC 3 cut(s) 117, 138, 1393
Eco31I GGTCTC 1 cut(s) 1549
Eco32I GATATC 1 cut(s) 1860
Eco47I GGWCC 3 cut(s) 120, 1419, 1691
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 2 cut(s) 575, 2240
Eco88I CYCGRG 2 cut(s) 1191, 2162
EcoICRI GAGCTC 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 707
EcoO109I RGGNCCY 2 cut(s) 1389, 1390
EcoRII CCWGG 3 cut(s) 129, 138, 1414
EcoRV GATATC 1 cut(s) 1860
EcoT14I CCWWGG 2 cut(s) 213, 1393
EcoT22I ATGCAT 2 cut(s) 774, 2147
EcoT38I GRGCYC 3 cut(s) 117, 138, 1393
EgeI GGCGCC 1 cut(s) 2244
EheI GGCGCC 1 cut(s) 2244
ErhI CCWWGG 2 cut(s) 213, 1393
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FaqI GGGAC 3 cut(s) 554, 814, 1432
FauI CCCGC 2 cut(s) 152, 231
FauNDI CATATG 2 cut(s) 1804, 2179
FbaI TGATCA 1 cut(s) 922
FblI GTMKAC 1 cut(s) 2106
Fnu4HI GCNGC 5 cut(s) 1221, 1286, 1725, 2068, 2348
FokI GGATG 6 cut(s) 627, 659, 956, 1070, 1973, 2051
FriOI GRGCYC 3 cut(s) 117, 138, 1393
Fsp4HI GCNGC 5 cut(s) 1221, 1286, 1725, 2068, 2348
FspBI CTAG 3 cut(s) 1251, 1991, 2307
GlaI GCGC 1 cut(s) 2244
GluI GCNGC 5 cut(s) 1221, 1286, 1725, 2068, 2348
GsaI CCCAGC 3 cut(s) 1043, 1556, 2136
GsuI CTGGAG 2 cut(s) 122, 2242
HaeII RGCGCY 1 cut(s) 2246
HaeIII GGCC 5 cut(s) 143, 212, 242, 1028, 1391
HapII CCGG 3 cut(s) 106, 118, 124
HgaI GACGC 1 cut(s) 1903
HhaI GCGC 1 cut(s) 2245
Hin1I GRCGYC 1 cut(s) 2243
Hin6I GCGC 1 cut(s) 2243
HinP1I GCGC 1 cut(s) 2243
HindIII AAGCTT 1 cut(s) 1844
HpaII CCGG 3 cut(s) 106, 118, 124
HphI GGTGA 6 cut(s) 646, 730, 1167, 1207, 1671, 1867
Hpy166II GTNNAC 2 cut(s) 782, 2107
Hpy188III TCNNGA 9 cut(s) 118, 629, 860, 967, 1094, 1567, 1850, 2084, 2162
Hpy8I GTNNAC 2 cut(s) 782, 2107
HpyCH4III ACNGT 4 cut(s) 828, 1126, 1246, 1450
HpyCH4IV ACGT 3 cut(s) 912, 1298, 1630
HpyF10VI GCNNNNNNNGC 3 cut(s) 769, 1478, 2353
HpyF3I CTNAG 6 cut(s) 194, 207, 272, 1236, 1593, 2126
HpySE526I ACGT 3 cut(s) 912, 1298, 1630
Hsp92I GRCGYC 1 cut(s) 2243
HspAI GCGC 1 cut(s) 2243
KasI GGCGCC 1 cut(s) 2242
Kpn2I TCCGGA 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 5 cut(s) 922, 1330, 1583, 1914, 2122
LmnI GCTCC 9 cut(s) 79, 120, 133, 141, 604, 727, 760, 2007, 2261
Lsp1109I GCAGC 2 cut(s) 1711, 2079
MaeI CTAG 3 cut(s) 1251, 1991, 2307
MaeII ACGT 3 cut(s) 912, 1298, 1630
MaeIII GTNAC 3 cut(s) 806, 1173, 2059
MalI GATC 5 cut(s) 924, 1332, 1585, 1916, 2124
MboI GATC 5 cut(s) 922, 1330, 1583, 1914, 2122
MflI RGATCY 2 cut(s) 1330, 1914
MhlI GDGCHC 3 cut(s) 117, 138, 1393
MluCI AATT 9 cut(s) 341, 590, 872, 887, 1017, 1046, 1182, 1762, 2227
MluI ACGCGT 1 cut(s) 2324
Mly113I GGCGCC 1 cut(s) 2243
MlyI GAGTC 7 cut(s) 92, 171, 265, 275, 454, 662, 1102
MmeI TCCRAC 5 cut(s) 159, 243, 798, 1638, 1948
Mph1103I ATGCAT 2 cut(s) 774, 2147
MroI TCCGGA 1 cut(s) 117
MroXI GAANNNNTTC 2 cut(s) 496, 991
MseI TTAA 7 cut(s) 312, 1023, 1185, 1293, 2021, 2072, 2097
MslI CAYNNNNRTG 4 cut(s) 942, 1611, 1902, 2192
MspA1I CMGCKG 1 cut(s) 2209
MspCI CTTAAG 1 cut(s) 1022
MspI CCGG 3 cut(s) 106, 118, 124
MspR9I CCNGG 5 cut(s) 106, 124, 131, 140, 1416
Mva1269I GAATGC 3 cut(s) 485, 704, 933
MvaI CCWGG 3 cut(s) 131, 140, 1416
MvnI CGCG 2 cut(s) 147, 2326
MwoI GCNNNNNNNGC 3 cut(s) 769, 1478, 2353
NarI GGCGCC 1 cut(s) 2243
NciI CCSGG 2 cut(s) 106, 124
NdeI CATATG 2 cut(s) 1804, 2179
NdeII GATC 5 cut(s) 922, 1330, 1583, 1914, 2122
NlaIV GGNNCC 8 cut(s) 116, 122, 128, 1391, 1420, 1455, 2244, 2257
NmeAIII GCCGAG 1 cut(s) 2219
NmuCI GTSAC 2 cut(s) 806, 1173
NsiI ATGCAT 2 cut(s) 774, 2147
NspI RCATGY 4 cut(s) 899, 1525, 1565, 1651
NspV TTCGAA 1 cut(s) 201
PaeR7I CTCGAG 1 cut(s) 2162
PagI TCATGA 2 cut(s) 966, 2083
PceI AGGCCT 1 cut(s) 1028
PciI ACATGT 3 cut(s) 1521, 1561, 1647
PctI GAATGC 3 cut(s) 485, 704, 933
PdmI GAANNNNTTC 2 cut(s) 496, 991
PflMI CCANNNNNTGG 2 cut(s) 793, 2252
PfoI TCCNGGA 1 cut(s) 129
PkrI GCNGC 5 cut(s) 1222, 1287, 1726, 2069, 2349
PleI GAGTC 7 cut(s) 92, 170, 264, 274, 454, 661, 1102
PluTI GGCGCC 1 cut(s) 2246
PpsI GAGTC 7 cut(s) 92, 170, 264, 274, 454, 661, 1102
Ppu21I YACGTR 2 cut(s) 913, 1299
PscI ACATGT 3 cut(s) 1521, 1561, 1647
PshAI GACNNNNGTC 1 cut(s) 546
PshBI ATTAAT 1 cut(s) 1185
PsiI TTATAA 1 cut(s) 1841
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 3 cut(s) 129, 138, 1414
PspFI CCCAGC 3 cut(s) 1039, 1552, 2132
PspGI CCWGG 3 cut(s) 129, 138, 1414
PspN4I GGNNCC 8 cut(s) 116, 122, 128, 1391, 1420, 1455, 2244, 2257
PspOMI GGGCCC 1 cut(s) 1389
PspPI GGNCC 7 cut(s) 120, 142, 240, 1389, 1390, 1419, 1691
PsuI RGATCY 2 cut(s) 1330, 1914
PvuII CAGCTG 1 cut(s) 2209
RsaI GTAC 2 cut(s) 1598, 2323
RsaNI GTAC 2 cut(s) 1597, 2322
RseI CAYNNNNRTG 4 cut(s) 942, 1611, 1902, 2192
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 7 cut(s) 312, 1023, 1185, 1293, 2021, 2072, 2097
SatI GCNGC 5 cut(s) 1221, 1286, 1725, 2068, 2348
Sau3AI GATC 5 cut(s) 922, 1330, 1583, 1914, 2122
Sau96I GGNCC 7 cut(s) 120, 142, 240, 1389, 1390, 1419, 1691
ScaI AGTACT 1 cut(s) 1598
SchI GAGTC 7 cut(s) 92, 171, 265, 275, 454, 662, 1102
ScrFI CCNGG 5 cut(s) 106, 124, 131, 140, 1416
SduI GDGCHC 3 cut(s) 117, 138, 1393
SfcI CTRYAG 1 cut(s) 1878
SfoI GGCGCC 1 cut(s) 2244
Sfr274I CTCGAG 1 cut(s) 2162
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 3 cut(s) 120, 1419, 1691
SlaI CTCGAG 1 cut(s) 2162
SmiMI CAYNNNNRTG 4 cut(s) 942, 1611, 1902, 2192
SmlI CTYRAG 4 cut(s) 629, 1022, 1850, 2162
SmoI CTYRAG 4 cut(s) 629, 1022, 1850, 2162
SnaBI TACGTA 2 cut(s) 913, 1299
SpeI ACTAGT 1 cut(s) 2306
Sse9I AATT 9 cut(s) 341, 590, 872, 887, 1017, 1046, 1182, 1762, 2227
SseBI AGGCCT 1 cut(s) 1028
SsiI CCGC 5 cut(s) 145, 238, 1220, 1285, 1682
SspDI GGCGCC 1 cut(s) 2242
SspMI CTAG 3 cut(s) 1251, 1991, 2307
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 1028
StyD4I CCNGG 5 cut(s) 104, 122, 129, 138, 1414
StyI CCWWGG 2 cut(s) 213, 1393
TaaI ACNGT 4 cut(s) 828, 1126, 1246, 1450
TaiI ACGT 3 cut(s) 915, 1301, 1633
TaqI TCGA 9 cut(s) 101, 201, 458, 470, 495, 500, 563, 1313, 2163
TasI AATT 9 cut(s) 341, 590, 872, 887, 1017, 1046, 1182, 1762, 2227
TatI WGTACW 1 cut(s) 1596
TauI GCSGC 2 cut(s) 1223, 1288
Tru1I TTAA 7 cut(s) 312, 1023, 1185, 1293, 2021, 2072, 2097
Tru9I TTAA 7 cut(s) 312, 1023, 1185, 1293, 2021, 2072, 2097
TscAI CASTG 3 cut(s) 700, 831, 1473
TseFI GTSAC 2 cut(s) 806, 1173
TseI GCWGC 3 cut(s) 1724, 2067, 2347
Tsp45I GTSAC 2 cut(s) 806, 1173
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 3 cut(s) 700, 831, 1473
Van91I CCANNNNNTGG 2 cut(s) 793, 2252
Vha464I CTTAAG 1 cut(s) 1022
VpaK11BI GGWCC 3 cut(s) 120, 1419, 1691
VspI ATTAAT 1 cut(s) 1185
XagI CCTNNNNNAGG 1 cut(s) 707
XapI RAATTY 1 cut(s) 1762
XceI RCATGY 4 cut(s) 899, 1525, 1565, 1651
XhoI CTCGAG 1 cut(s) 2162
XmiI GTMKAC 1 cut(s) 2106
XmnI GAANNNNTTC 2 cut(s) 496, 991
XspI CTAG 3 cut(s) 1251, 1991, 2307
ZrmI AGTACT 1 cut(s) 1598
Zsp2I ATGCAT 2 cut(s) 774, 2147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.