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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 187 |
| Acc36I | ACCTGC | 1 cut(s) | 187 |
| AciI | CCGC | 1 cut(s) | 246 |
| AclWI | GGATC | 3 cut(s) | 148, 161, 535 |
| AcsI | RAATTY | 3 cut(s) | 298, 361, 416 |
| AcvI | CACGTG | 1 cut(s) | 658 |
| AfaI | GTAC | 1 cut(s) | 639 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 21, 163 |
| AflIII | ACRYGT | 1 cut(s) | 655 |
| AgsI | TTSAA | 6 cut(s) | 98, 272, 338, 569, 613, 768 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 307, 339 |
| AluBI | AGCT | 5 cut(s) | 94, 116, 212, 706, 759 |
| AluI | AGCT | 5 cut(s) | 94, 116, 212, 706, 759 |
| AlwI | GGATC | 3 cut(s) | 148, 161, 535 |
| Ama87I | CYCGRG | 1 cut(s) | 476 |
| AoxI | GGCC | 1 cut(s) | 501 |
| ApeKI | GCWGC | 3 cut(s) | 11, 116, 186 |
| ApoI | RAATTY | 3 cut(s) | 298, 361, 416 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 513, 677 |
| AspS9I | GGNCC | 1 cut(s) | 502 |
| AsuHPI | GGTGA | 2 cut(s) | 152, 725 |
| AvaI | CYCGRG | 1 cut(s) | 476 |
| BamHI | GGATCC | 1 cut(s) | 153 |
| BbrPI | CACGTG | 1 cut(s) | 658 |
| BbvI | GCAGC | 3 cut(s) | 23, 103, 198 |
| BccI | CCATC | 1 cut(s) | 446 |
| BciVI | GTATCC | 1 cut(s) | 655 |
| BfaI | CTAG | 5 cut(s) | 294, 341, 357, 506, 707 |
| BfuAI | ACCTGC | 1 cut(s) | 187 |
| BfuI | GTATCC | 1 cut(s) | 655 |
| BisI | GCNGC | 4 cut(s) | 12, 117, 187, 246 |
| BlsI | GCNGC | 4 cut(s) | 13, 118, 188, 247 |
| BmeT110I | CYCGRG | 1 cut(s) | 476 |
| BmgT120I | GGNCC | 1 cut(s) | 502 |
| BmiI | GGNNCC | 1 cut(s) | 155 |
| BmrI | ACTGGG | 3 cut(s) | 242, 629, 675 |
| BmsI | GCATC | 3 cut(s) | 280, 343, 538 |
| BmuI | ACTGGG | 3 cut(s) | 242, 629, 675 |
| BsaAI | YACGTR | 1 cut(s) | 658 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 511, 541 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 21, 163 |
| Bse1I | ACTGG | 4 cut(s) | 237, 635, 670, 682 |
| BseGI | GGATG | 1 cut(s) | 675 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 21, 163 |
| BseMII | CTCAG | 4 cut(s) | 121, 204, 300, 503 |
| BseNI | ACTGG | 4 cut(s) | 237, 635, 670, 682 |
| BseRI | GAGGAG | 1 cut(s) | 104 |
| BseXI | GCAGC | 3 cut(s) | 23, 103, 198 |
| BshFI | GGCC | 1 cut(s) | 503 |
| BsiHKCI | CYCGRG | 1 cut(s) | 476 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 21, 163 |
| BsmI | GAATGC | 1 cut(s) | 247 |
| BsnI | GGCC | 1 cut(s) | 503 |
| BsoBI | CYCGRG | 1 cut(s) | 476 |
| Bsp143I | GATC | 3 cut(s) | 153, 540, 749 |
| BspACI | CCGC | 1 cut(s) | 246 |
| BspANI | GGCC | 1 cut(s) | 503 |
| BspCNI | CTCAG | 4 cut(s) | 120, 205, 299, 502 |
| BspLI | GGNNCC | 1 cut(s) | 155 |
| BspMI | ACCTGC | 1 cut(s) | 187 |
| BspPI | GGATC | 3 cut(s) | 148, 161, 535 |
| BsrI | ACTGG | 4 cut(s) | 237, 635, 670, 682 |
| BssMI | GATC | 3 cut(s) | 153, 540, 749 |
| Bst4CI | ACNGT | 2 cut(s) | 7, 135 |
| Bst6I | CTCTTC | 1 cut(s) | 108 |
| BstBAI | YACGTR | 1 cut(s) | 658 |
| BstC8I | GCNNGC | 2 cut(s) | 184, 733 |
| BstDEI | CTNAG | 6 cut(s) | 107, 213, 286, 432, 489, 753 |
| BstF5I | GGATG | 1 cut(s) | 675 |
| BstKTI | GATC | 3 cut(s) | 156, 543, 752 |
| BstMBI | GATC | 3 cut(s) | 153, 540, 749 |
| BstV1I | GCAGC | 3 cut(s) | 23, 103, 198 |
| BstX2I | RGATCY | 2 cut(s) | 153, 540 |
| BstYI | RGATCY | 2 cut(s) | 153, 540 |
| BsuI | GTATCC | 1 cut(s) | 655 |
| BsuRI | GGCC | 1 cut(s) | 503 |
| BtsCI | GGATG | 1 cut(s) | 675 |
| BtsIMutI | CAGTG | 1 cut(s) | 230 |
| BveI | ACCTGC | 1 cut(s) | 187 |
| Cac8I | GCNNGC | 2 cut(s) | 184, 733 |
| Cfr13I | GGNCC | 1 cut(s) | 502 |
| Csp6I | GTAC | 1 cut(s) | 638 |
| CviAII | CATG | 1 cut(s) | 346 |
| CviQI | GTAC | 1 cut(s) | 638 |
| DdeI | CTNAG | 6 cut(s) | 107, 213, 286, 432, 489, 753 |
| DpnI | GATC | 3 cut(s) | 155, 542, 751 |
| DpnII | GATC | 3 cut(s) | 153, 540, 749 |
| Eam1104I | CTCTTC | 1 cut(s) | 108 |
| EarI | CTCTTC | 1 cut(s) | 108 |
| Eco72I | CACGTG | 1 cut(s) | 658 |
| Eco88I | CYCGRG | 1 cut(s) | 476 |
| EcoO109I | RGGNCCY | 1 cut(s) | 502 |
| EcoRI | GAATTC | 1 cut(s) | 298 |
| EcoT22I | ATGCAT | 1 cut(s) | 774 |
| FaeI | CATG | 1 cut(s) | 349 |
| FaiI | YATR | 8 cut(s) | 61, 63, 150, 347, 513, 554, 741, 743 |
| FatI | CATG | 1 cut(s) | 345 |
| FauNDI | CATATG | 1 cut(s) | 61 |
| Fnu4HI | GCNGC | 4 cut(s) | 12, 117, 187, 246 |
| FokI | GGATG | 1 cut(s) | 682 |
| Fsp4HI | GCNGC | 4 cut(s) | 12, 117, 187, 246 |
| FspBI | CTAG | 5 cut(s) | 294, 341, 357, 506, 707 |
| GluI | GCNGC | 4 cut(s) | 12, 117, 187, 246 |
| HaeIII | GGCC | 1 cut(s) | 503 |
| Hin1II | CATG | 1 cut(s) | 349 |
| HinfI | GANTC | 4 cut(s) | 224, 397, 559, 764 |
| HphI | GGTGA | 2 cut(s) | 152, 725 |
| Hpy188I | TCNGA | 5 cut(s) | 82, 110, 289, 492, 519 |
| Hpy188III | TCNNGA | 1 cut(s) | 125 |
| HpyAV | CCTTC | 1 cut(s) | 619 |
| HpyCH4III | ACNGT | 2 cut(s) | 7, 135 |
| HpyCH4IV | ACGT | 1 cut(s) | 657 |
| HpyCH4V | TGCA | 5 cut(s) | 59, 182, 606, 731, 772 |
| HpyF3I | CTNAG | 6 cut(s) | 107, 213, 286, 432, 489, 753 |
| HpySE526I | ACGT | 1 cut(s) | 657 |
| Hsp92II | CATG | 1 cut(s) | 349 |
| Kzo9I | GATC | 3 cut(s) | 153, 540, 749 |
| LmnI | GCTCC | 1 cut(s) | 91 |
| Lsp1109I | GCAGC | 3 cut(s) | 23, 103, 198 |
| LweI | GCATC | 3 cut(s) | 280, 343, 538 |
| MaeI | CTAG | 5 cut(s) | 294, 341, 357, 506, 707 |
| MaeII | ACGT | 1 cut(s) | 657 |
| MaeIII | GTNAC | 2 cut(s) | 52, 684 |
| MalI | GATC | 3 cut(s) | 155, 542, 751 |
| MboI | GATC | 3 cut(s) | 153, 540, 749 |
| MboII | GAAGA | 6 cut(s) | 95, 179, 219, 249, 260, 315 |
| MflI | RGATCY | 2 cut(s) | 153, 540 |
| MluCI | AATT | 6 cut(s) | 19, 143, 298, 361, 416, 618 |
| MlyI | GAGTC | 1 cut(s) | 553 |
| MmeI | TCCRAC | 2 cut(s) | 6, 338 |
| MnlI | CCTC | 9 cut(s) | 22, 82, 82, 211, 295, 567, 580, 653, 703 |
| Mph1103I | ATGCAT | 1 cut(s) | 774 |
| MroXI | GAANNNNTTC | 2 cut(s) | 513, 677 |
| MseI | TTAA | 4 cut(s) | 617, 647, 723, 775 |
| Mva1269I | GAATGC | 1 cut(s) | 247 |
| NdeI | CATATG | 1 cut(s) | 61 |
| NdeII | GATC | 3 cut(s) | 153, 540, 749 |
| NlaIII | CATG | 1 cut(s) | 349 |
| NlaIV | GGNNCC | 1 cut(s) | 155 |
| NmeAIII | GCCGAG | 1 cut(s) | 40 |
| NmuCI | GTSAC | 1 cut(s) | 684 |
| NsiI | ATGCAT | 1 cut(s) | 774 |
| PaqCI | CACCTGC | 1 cut(s) | 187 |
| PctI | GAATGC | 1 cut(s) | 247 |
| PdmI | GAANNNNTTC | 2 cut(s) | 513, 677 |
| PfeI | GAWTC | 3 cut(s) | 224, 397, 764 |
| PkrI | GCNGC | 4 cut(s) | 13, 118, 188, 247 |
| PleI | GAGTC | 1 cut(s) | 553 |
| PmaCI | CACGTG | 1 cut(s) | 658 |
| PmlI | CACGTG | 1 cut(s) | 658 |
| PpsI | GAGTC | 1 cut(s) | 553 |
| Ppu21I | YACGTR | 1 cut(s) | 658 |
| PspCI | CACGTG | 1 cut(s) | 658 |
| PspN4I | GGNNCC | 1 cut(s) | 155 |
| PspPI | GGNCC | 1 cut(s) | 502 |
| PsuI | RGATCY | 2 cut(s) | 153, 540 |
| RsaI | GTAC | 1 cut(s) | 639 |
| RsaNI | GTAC | 1 cut(s) | 638 |
| SaqAI | TTAA | 4 cut(s) | 617, 647, 723, 775 |
| SatI | GCNGC | 4 cut(s) | 12, 117, 187, 246 |
| Sau3AI | GATC | 3 cut(s) | 153, 540, 749 |
| Sau96I | GGNCC | 1 cut(s) | 502 |
| SchI | GAGTC | 1 cut(s) | 553 |
| SfaNI | GCATC | 3 cut(s) | 280, 343, 538 |
| Sse9I | AATT | 6 cut(s) | 19, 143, 298, 361, 416, 618 |
| SsiI | CCGC | 1 cut(s) | 246 |
| SspI | AATATT | 1 cut(s) | 334 |
| SspMI | CTAG | 5 cut(s) | 294, 341, 357, 506, 707 |
| TaaI | ACNGT | 2 cut(s) | 7, 135 |
| TaiI | ACGT | 1 cut(s) | 660 |
| TaqI | TCGA | 1 cut(s) | 748 |
| TasI | AATT | 6 cut(s) | 19, 143, 298, 361, 416, 618 |
| TauI | GCSGC | 1 cut(s) | 248 |
| TfiI | GAWTC | 3 cut(s) | 224, 397, 764 |
| Tru1I | TTAA | 4 cut(s) | 617, 647, 723, 775 |
| Tru9I | TTAA | 4 cut(s) | 617, 647, 723, 775 |
| TscAI | CASTG | 1 cut(s) | 237 |
| TseFI | GTSAC | 1 cut(s) | 684 |
| TseI | GCWGC | 3 cut(s) | 11, 116, 186 |
| Tsp45I | GTSAC | 1 cut(s) | 684 |
| TspDTI | ATGAA | 2 cut(s) | 429, 686 |
| TspGWI | ACGGA | 2 cut(s) | 179, 595 |
| TspRI | CASTG | 1 cut(s) | 237 |
| XapI | RAATTY | 3 cut(s) | 298, 361, 416 |
| XmnI | GAANNNNTTC | 2 cut(s) | 513, 677 |
| XspI | CTAG | 5 cut(s) | 294, 341, 357, 506, 707 |
| Zsp2I | ATGCAT | 1 cut(s) | 774 |