Rroxscaffold_6G00430090

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000126
Physical Location & Seq
Forward (+)
50295926 .. 50300796
4871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430090.1

Sequence Viewer

Length: 2247 bp
ATGAGTGAGGAAGTGGAGGAATTTCGTGTGCGTAGGAAGGAAATGGATGAGTTGAAATGTAAACTTGAGGCGAAGGAGAGTGAGCTGGAGAGATGGGCTGTGGCCCTTGCATTGAAAGAGAAACAAGTTAAATCGAGAAGTAAGGAACTTAGTTTGACTGAAAAGAGGGTCGTGGAAAGCCTCAGTGAGGTTCAGATGAAAGAAAAGGGTTTTGATGAGCAGAAAAAATTGATAGAAGAAGGTAGAAAGCATTTGGATGTAGTCTCTAATAAACTACAAATGAAAGAGTGGAGACTTCGTAAACAAGGAAAAGACCTTAAATTGGAAAAACAAGTATTGGAATCGAATAAAAAATCCACTAAAGAACACAAACAGGGTCCTAAATCGCAAGAGGAGCCTAACGCCCTCCATTCTGAATCTGAAATAACTTGGAATGGTATAGGGTTGCAGAGGTTCATGATTGGGCAGTTGCAGAGAATAGATTTAATGGGTAGTAAAATGTCAGCTGTTCTTCAAGGCTCATCAGACCCGGCTAAATTAGTGTTGGCTGCAATGCAAGGATTTCACAGGTCAAATTCAAATACGGACAGCAGGGAGATTGAATCTGATCTGGAAATTACTAGAAGGAGTAGCATTCTTTTGCTGGAGGAGCTGAAGAGTATGTCAGCACAAATCAGTCCTCCAGTGAAAGAAGAAGCCATGAAGTTGGCCACTGAGTGGAAGTCTAAGATAGCAGAGACGACTAATAATAGATTAGAGATATTGGGGTTTCTGCAGCTTGTTACTACGTATGAGTTGACCTCTCTTTATGATGCAAAGGAACTTCAAAGTCTTTTAGCTTTAATTGCTACGCCTAATCAAGCAACTGAATGCCAGGCCCTTGCTATCTCTGATAAGCCATCTGGAATCAGTATGTGTCCTCCTGTGAAAGATAAGGAACAAGAATCTTCACTGGCCAGAAATGCAGTAACTTCTTCGAATCTTCAACCAAGTGCCAACGTACATGCAAGGAATTCTCCAGGGTTCGTAAATGATCAATTGGCTTGGAAATATTCAATACTGAACAAAATTTTTGTTGCTCTTGAAAAGTCATCAAATTTAAACCCAGCAAAACTTTTGTTGGATTTGATGCAAGTATCCTATGCTGGATACTGGACACAAGGAAATGCTGGTTTTGAAGCAACTGCCATGAAGAGCTTCATTTCCCTATTGGAGACTGTAAGAAGAGCCCAACCGCATGTTGGAACTCAATTGAAAGAGGATGCAGCACAGTTAGCTGTACAATGGAAAAAGAAACTGACTGATGCTGAAAATCCCCTAGATATCTTGGGTTTCTTGATGTTCATAACTACGTATGGATTGCATTCTACGTTCACGACAGATGAGCTTGCAAAGCTTTTTGGGCTGATTTCTCGGCATACACATGCTTTAGAACTATGTCAGACAATTGGTTATGCACAGAAATTCCCTGATTATATTCTGGATCTTGTTCAAAGCAAACAACTAGTTGAGGCTGTCAGATTAATTTGCACCTCCAAATTAACCGACAAATTCCCCCCAGCACCACTGTTAGGTGAATATGTGGAGGATTCATTGAATCGTTGCAGTAAAATTTGCACGACAAAGATATCATTTGATGAAAAGGATAAGGTTGTAGACAGCCTAATAGCTGATCTAAGATCTGTGATCCAATGCATCAACAAATACAATCTCGAGTCTGAATACCAATCCGCACATGTTGAAATACAAATAGATGAACTGAAAAAACTAAAGGAGAATTGGAGACTGTCGGCACAATCTCTTGCCTCCCAGGTTGAACCACAAAAGAGTAAAGGGAAGAAGCGTAGTTGTCCTAGCACTCCTACACCCATGTTTCAACCACAACAGCAGCTTGGACATAATGGTCACCTGACAGCCATATCAGCTGCTAGACCGCCATCCTATCCATTGCCTACTTGTATCCCAAGCTATCCACAGTCACATTCGTCATCTGTGCTGAAAACTAATTTTGGAATTCCCGGGCAGTTTACTATGCCTGCCAAGAGCCACCCATTTGCTGCTAATATTGGAGGGCCGTTCATGCCAACCATGAAGCGCCCGTGTATGATGACTACCAGATACCCAGCCTCAGGCTCAGCCCCAGCTCCAACTAGGGCCCCGGCTCCAACTCCAGTGCCAATGCACAGTGCTAATTTCTGTGTACCATGCAAGTTTCCTCACCCTAATATGATGCAGTTTCGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

748

Amino Acids

83.82

Weight (kDa)

8.91

Isoelectric Point (pI)

55.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 145 - 298 6.1e-30 Frigida-like protein
Frigida PF07899 340 - 594 9.7e-52 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1902
AccB7I CCANNNNNTGG 1 cut(s) 717
AccI GTMKAC 1 cut(s) 1656
AciI CCGC 3 cut(s) 1235, 1731, 1934
AclWI GGATC 2 cut(s) 1491, 1681
AcoI YGGCCR 2 cut(s) 708, 954
AcsI RAATTY 9 cut(s) 20, 574, 1012, 1068, 1096, 1463, 1550, 1611, 2013
AcuI CTGAAG 1 cut(s) 674
AdeI CACNNNGTG 1 cut(s) 717
AfaI GTAC 3 cut(s) 1002, 1281, 2202
AfiI CCNNNNNNNGG 6 cut(s) 187, 322, 717, 1241, 1571, 2129
AflIII ACRYGT 1 cut(s) 1735
AhlI ACTAGT 1 cut(s) 1504
AjnI CCWGG 3 cut(s) 873, 1018, 1809
Alw26I GTCTC 5 cut(s) 268, 286, 731, 1208, 1777
AlwI GGATC 2 cut(s) 1491, 1681
Ama87I CYCGRG 2 cut(s) 1712, 2018
AoxI GGCC 6 cut(s) 102, 708, 876, 954, 2072, 2154
ApaI GGGCCC 1 cut(s) 2158
ApeKI GCWGC 6 cut(s) 548, 775, 1265, 1888, 1925, 2057
ApoI RAATTY 9 cut(s) 20, 574, 1012, 1068, 1096, 1463, 1550, 1611, 2013
AseI ATTAAT 1 cut(s) 1523
Asp700I GAANNNNTTC 1 cut(s) 1196
AspLEI GCGC 1 cut(s) 2097
AspS9I GGNCC 6 cut(s) 103, 377, 877, 2072, 2154, 2155
AsuC2I CCSGG 4 cut(s) 530, 2019, 2020, 2159
AsuHPI GGTGA 3 cut(s) 1586, 1898, 2210
AsuII TTCGAA 1 cut(s) 977
AvaI CYCGRG 2 cut(s) 1712, 2018
AvaII GGWCC 1 cut(s) 377
AxyI CCTNAGG 1 cut(s) 2128
BaeGI GKGCMC 1 cut(s) 2158
BalI TGGCCA 2 cut(s) 710, 956
BanII GRGCYC 2 cut(s) 1231, 2158
BbvI GCAGC 6 cut(s) 535, 787, 1277, 1900, 1912, 2044
BccI CCATC 3 cut(s) 87, 907, 1945
BceAI ACGGC 1 cut(s) 2059
BciT130I CCWGG 3 cut(s) 875, 1020, 1811
BciVI GTATCC 3 cut(s) 1142, 1147, 1970
BclI TGATCA 1 cut(s) 1033
BcnI CCSGG 4 cut(s) 530, 2019, 2020, 2159
BcoDI GTCTC 5 cut(s) 268, 286, 731, 1208, 1777
BcuI ACTAGT 1 cut(s) 1504
BfaI CTAG 6 cut(s) 621, 1319, 1505, 1854, 1929, 2151
BfmI CTRYAG 1 cut(s) 773
BfoI RGCGCY 1 cut(s) 2098
BfuI GTATCC 3 cut(s) 1142, 1147, 1970
BglII AGATCT 1 cut(s) 1679
BisI GCNGC 6 cut(s) 549, 776, 1266, 1889, 1926, 2058
BlpI GCTNAGC 1 cut(s) 2134
BlsI GCNGC 6 cut(s) 550, 777, 1267, 1890, 1927, 2059
Bme1390I CCNGG 7 cut(s) 530, 875, 1020, 1811, 2019, 2020, 2159
Bme18I GGWCC 1 cut(s) 377
BmeT110I CYCGRG 2 cut(s) 1712, 2018
BmgT120I GGNCC 6 cut(s) 103, 377, 877, 2072, 2154, 2155
BmiI GGNNCC 5 cut(s) 378, 396, 2156, 2157, 2163
BmrFI CCNGG 7 cut(s) 530, 875, 1020, 1811, 2019, 2020, 2159
BmsI GCATC 6 cut(s) 802, 1119, 1252, 1294, 1704, 2220
BplI GAGNNNNNCTC 2 cut(s) 785, 817
BpmI CTGGAG 5 cut(s) 107, 665, 666, 1002, 2154
Bpu1102I GCTNAGC 1 cut(s) 2134
Bpu14I TTCGAA 1 cut(s) 977
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 4 cut(s) 530, 2019, 2020, 2159
BsaAI YACGTR 2 cut(s) 789, 1353
BsaJI CCNNGG 4 cut(s) 1019, 1809, 2018, 2157
BsaXI ACNNNNNCTCC 2 cut(s) 283, 313
Bsc4I CCNNNNNNNGG 6 cut(s) 187, 322, 717, 1241, 1571, 2129
Bse1I ACTGG 4 cut(s) 683, 957, 1157, 2171
Bse21I CCTNAGG 1 cut(s) 2128
Bse3DI GCAATG 2 cut(s) 558, 1946
BseBI CCWGG 3 cut(s) 875, 1020, 1811
BseDI CCNNGG 4 cut(s) 1019, 1809, 2018, 2157
BseGI GGATG 4 cut(s) 52, 262, 1267, 1937
BseLI CCNNNNNNNGG 6 cut(s) 187, 322, 717, 1241, 1571, 2129
BseMI GCAATG 2 cut(s) 558, 1946
BseMII CTCAG 4 cut(s) 196, 705, 2142, 2148
BseNI ACTGG 4 cut(s) 683, 957, 1157, 2171
BseRI GAGGAG 2 cut(s) 407, 662
BseSI GKGCMC 1 cut(s) 2158
BseXI GCAGC 6 cut(s) 535, 787, 1277, 1900, 1912, 2044
BseYI CCCAGC 4 cut(s) 1105, 1558, 2122, 2140
BshFI GGCC 6 cut(s) 104, 710, 878, 956, 2074, 2156
BsiHKCI CYCGRG 2 cut(s) 1712, 2018
BsiSI CCGG 3 cut(s) 530, 2019, 2159
BslI CCNNNNNNNGG 6 cut(s) 187, 322, 717, 1241, 1571, 2129
BsmAI GTCTC 5 cut(s) 268, 286, 731, 1208, 1777
BsmBI CGTCTC 1 cut(s) 731
BsmI GAATGC 3 cut(s) 633, 875, 1363
BsnI GGCC 6 cut(s) 104, 710, 878, 956, 2074, 2156
BsoBI CYCGRG 2 cut(s) 1712, 2018
Bsp119I TTCGAA 1 cut(s) 977
Bsp120I GGGCCC 1 cut(s) 2154
Bsp1286I GDGCHC 2 cut(s) 1231, 2158
Bsp1407I TGTACA 1 cut(s) 1279
Bsp143I GATC 6 cut(s) 607, 1033, 1483, 1672, 1679, 1686
Bsp1720I GCTNAGC 1 cut(s) 2134
BspACI CCGC 3 cut(s) 1235, 1731, 1934
BspANI GGCC 6 cut(s) 104, 710, 878, 956, 2074, 2156
BspCNI CTCAG 4 cut(s) 195, 706, 2141, 2147
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 5 cut(s) 378, 396, 2156, 2157, 2163
BspMAI CTGCAG 1 cut(s) 777
BspPI GGATC 2 cut(s) 1491, 1681
BspQI GCTCTTC 2 cut(s) 1187, 1219
BspT104I TTCGAA 1 cut(s) 977
BsrDI GCAATG 2 cut(s) 558, 1946
BsrGI TGTACA 1 cut(s) 1279
BsrI ACTGG 4 cut(s) 683, 957, 1157, 2171
BssECI CCNNGG 4 cut(s) 1019, 1809, 2018, 2157
BssMI GATC 6 cut(s) 607, 1033, 1483, 1672, 1679, 1686
Bst2UI CCWGG 3 cut(s) 875, 1020, 1811
Bst4CI ACNGT 6 cut(s) 1219, 1272, 1569, 1788, 1977, 2186
Bst6I CTCTTC 3 cut(s) 650, 1187, 1219
BstAUI TGTACA 1 cut(s) 1279
BstBAI YACGTR 2 cut(s) 789, 1353
BstBI TTCGAA 1 cut(s) 977
BstC8I GCNNGC 2 cut(s) 1389, 2037
BstDEI CTNAG 7 cut(s) 149, 182, 714, 726, 1676, 2128, 2134
BstEII GGTNACC 1 cut(s) 1904
BstENI CCTNNNNNAGG 1 cut(s) 185
BstF5I GGATG 4 cut(s) 52, 262, 1267, 1937
BstH2I RGCGCY 1 cut(s) 2098
BstHHI GCGC 1 cut(s) 2097
BstKTI GATC 6 cut(s) 610, 1036, 1486, 1675, 1682, 1689
BstMAI GTCTC 5 cut(s) 268, 286, 731, 1208, 1777
BstMBI GATC 6 cut(s) 607, 1033, 1483, 1672, 1679, 1686
BstMWI GCNNNNNNNGC 9 cut(s) 394, 649, 845, 962, 1274, 1393, 1402, 1922, 2080
BstNI CCWGG 3 cut(s) 875, 1020, 1811
BstNSI RCATGY 4 cut(s) 1007, 1241, 1427, 1739
BstPI GGTNACC 1 cut(s) 1904
BstSCI CCNGG 7 cut(s) 528, 873, 1018, 1809, 2017, 2018, 2157
BstSFI CTRYAG 1 cut(s) 773
BstSLI GKGCMC 1 cut(s) 2158
BstSNI TACGTA 2 cut(s) 789, 1353
BstV1I GCAGC 6 cut(s) 535, 787, 1277, 1900, 1912, 2044
BstX2I RGATCY 2 cut(s) 1483, 1679
BstXI CCANNNNNNTGG 1 cut(s) 706
BstYI RGATCY 2 cut(s) 1483, 1679
Bsu36I CCTNAGG 1 cut(s) 2128
BsuI GTATCC 3 cut(s) 1142, 1147, 1970
BsuRI GGCC 6 cut(s) 104, 710, 878, 956, 2074, 2156
BtsCI GGATG 4 cut(s) 52, 262, 1267, 1937
BtsIMutI CAGTG 7 cut(s) 190, 690, 711, 950, 1565, 2178, 2191
Cac8I GCNNGC 2 cut(s) 1389, 2037
CciI TCATGA 1 cut(s) 456
CfoI GCGC 1 cut(s) 2097
Cfr13I GGNCC 6 cut(s) 103, 377, 877, 2072, 2154, 2155
Cfr9I CCCGGG 1 cut(s) 2018
Csp6I GTAC 3 cut(s) 1001, 1280, 2201
CviQI GTAC 3 cut(s) 1001, 1280, 2201
DdeI CTNAG 7 cut(s) 149, 182, 714, 726, 1676, 2128, 2134
DpnI GATC 6 cut(s) 609, 1035, 1485, 1674, 1681, 1688
DpnII GATC 6 cut(s) 607, 1033, 1483, 1672, 1679, 1686
DraI TTTAAA 1 cut(s) 1101
DraIII CACNNNGTG 1 cut(s) 717
DrdI GACNNNNNNGTC 1 cut(s) 1902
DseDI GACNNNNNNGTC 1 cut(s) 1902
EaeI YGGCCR 2 cut(s) 708, 954
Eam1104I CTCTTC 3 cut(s) 650, 1187, 1219
EarI CTCTTC 3 cut(s) 650, 1187, 1219
Eco105I TACGTA 2 cut(s) 789, 1353
Eco24I GRGCYC 2 cut(s) 1231, 2158
Eco32I GATATC 2 cut(s) 1324, 1629
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 674
Eco81I CCTNAGG 1 cut(s) 2128
Eco88I CYCGRG 2 cut(s) 1712, 2018
Eco91I GGTNACC 1 cut(s) 1904
EcoNI CCTNNNNNAGG 1 cut(s) 185
EcoO109I RGGNCCY 4 cut(s) 377, 877, 2154, 2155
EcoO65I GGTNACC 1 cut(s) 1904
EcoRI GAATTC 2 cut(s) 1012, 2013
EcoRII CCWGG 3 cut(s) 873, 1018, 1809
EcoRV GATATC 2 cut(s) 1324, 1629
EcoT22I ATGCAT 1 cut(s) 1697
EcoT38I GRGCYC 2 cut(s) 1231, 2158
Esp3I CGTCTC 1 cut(s) 731
FbaI TGATCA 1 cut(s) 1033
FblI GTMKAC 1 cut(s) 1656
Fnu4HI GCNGC 6 cut(s) 549, 776, 1266, 1889, 1926, 2058
FokI GGATG 4 cut(s) 59, 269, 1274, 1924
FriOI GRGCYC 2 cut(s) 1231, 2158
Fsp4HI GCNGC 6 cut(s) 549, 776, 1266, 1889, 1926, 2058
FspBI CTAG 6 cut(s) 621, 1319, 1505, 1854, 1929, 2151
GlaI GCGC 1 cut(s) 2096
GluI GCNGC 6 cut(s) 549, 776, 1266, 1889, 1926, 2058
GsaI CCCAGC 4 cut(s) 1109, 1562, 2126, 2144
GsuI CTGGAG 5 cut(s) 107, 665, 666, 1002, 2154
HaeII RGCGCY 1 cut(s) 2098
HaeIII GGCC 6 cut(s) 104, 710, 878, 956, 2074, 2156
HapII CCGG 3 cut(s) 530, 2019, 2159
HhaI GCGC 1 cut(s) 2097
Hin6I GCGC 1 cut(s) 2095
HinP1I GCGC 1 cut(s) 2095
HincII GTYRAC 1 cut(s) 798
HindII GTYRAC 1 cut(s) 798
HindIII AAGCTT 1 cut(s) 1394
HinfI GANTC 9 cut(s) 341, 416, 602, 906, 944, 979, 1589, 1597, 1715
HpaII CCGG 3 cut(s) 530, 2019, 2159
HphI GGTGA 3 cut(s) 1586, 1898, 2210
Hpy166II GTNNAC 7 cut(s) 62, 302, 798, 1374, 1657, 2028, 2201
Hpy188III TCNNGA 9 cut(s) 135, 457, 611, 903, 1082, 1336, 1375, 1481, 1712
Hpy8I GTNNAC 7 cut(s) 62, 302, 798, 1374, 1657, 2028, 2201
HpyAV CCTTC 4 cut(s) 31, 67, 233, 618
HpyCH4III ACNGT 6 cut(s) 1219, 1272, 1569, 1788, 1977, 2186
HpyCH4IV ACGT 4 cut(s) 788, 999, 1352, 1370
HpyF10VI GCNNNNNNNGC 9 cut(s) 394, 649, 845, 962, 1274, 1393, 1402, 1922, 2080
HpyF3I CTNAG 7 cut(s) 149, 182, 714, 726, 1676, 2128, 2134
HpySE526I ACGT 4 cut(s) 788, 999, 1352, 1370
HspAI GCGC 1 cut(s) 2095
Ksp22I TGATCA 1 cut(s) 1033
Kzo9I GATC 6 cut(s) 607, 1033, 1483, 1672, 1679, 1686
LguI GCTCTTC 2 cut(s) 1187, 1219
LmnI GCTCC 4 cut(s) 394, 649, 2149, 2167
Lsp1109I GCAGC 6 cut(s) 535, 787, 1277, 1900, 1912, 2044
LweI GCATC 6 cut(s) 802, 1119, 1252, 1294, 1704, 2220
MaeI CTAG 6 cut(s) 621, 1319, 1505, 1854, 1929, 2151
MaeII ACGT 4 cut(s) 788, 999, 1352, 1370
MaeIII GTNAC 4 cut(s) 781, 967, 1904, 1977
MalI GATC 6 cut(s) 609, 1035, 1485, 1674, 1681, 1688
MboI GATC 6 cut(s) 607, 1033, 1483, 1672, 1679, 1686
MfeI CAATTG 3 cut(s) 1037, 1250, 1446
MflI RGATCY 2 cut(s) 1483, 1679
MhlI GDGCHC 2 cut(s) 1231, 2158
MlsI TGGCCA 2 cut(s) 710, 956
MluNI TGGCCA 2 cut(s) 710, 956
MlyI GAGTC 1 cut(s) 1724
MmeI TCCRAC 4 cut(s) 1101, 1222, 2171, 2189
Mox20I TGGCCA 2 cut(s) 710, 956
Mph1103I ATGCAT 1 cut(s) 1697
MroXI GAANNNNTTC 1 cut(s) 1196
MscI TGGCCA 2 cut(s) 710, 956
MseI TTAA 7 cut(s) 129, 318, 485, 842, 1100, 1523, 1541
MslI CAYNNNNRTG 2 cut(s) 255, 1422
Msp20I TGGCCA 2 cut(s) 710, 956
MspA1I CMGCKG 2 cut(s) 506, 1925
MspI CCGG 3 cut(s) 530, 2019, 2159
MspR9I CCNGG 7 cut(s) 530, 875, 1020, 1811, 2019, 2020, 2159
MunI CAATTG 3 cut(s) 1037, 1250, 1446
Mva1269I GAATGC 3 cut(s) 633, 875, 1363
MvaI CCWGG 3 cut(s) 875, 1020, 1811
MwoI GCNNNNNNNGC 9 cut(s) 394, 649, 845, 962, 1274, 1393, 1402, 1922, 2080
NciI CCSGG 4 cut(s) 530, 2019, 2020, 2159
NdeII GATC 6 cut(s) 607, 1033, 1483, 1672, 1679, 1686
NlaIV GGNNCC 5 cut(s) 378, 396, 2156, 2157, 2163
NmeAIII GCCGAG 1 cut(s) 1393
NmuCI GTSAC 2 cut(s) 1904, 1977
NsiI ATGCAT 1 cut(s) 1697
NspI RCATGY 4 cut(s) 1007, 1241, 1427, 1739
NspV TTCGAA 1 cut(s) 977
PaeR7I CTCGAG 1 cut(s) 1712
PagI TCATGA 1 cut(s) 456
PciI ACATGT 1 cut(s) 1735
PciSI GCTCTTC 2 cut(s) 1187, 1219
PctI GAATGC 3 cut(s) 633, 875, 1363
PdmI GAANNNNTTC 1 cut(s) 1196
PfeI GAWTC 8 cut(s) 341, 416, 602, 906, 944, 979, 1589, 1597
PflMI CCANNNNNTGG 1 cut(s) 717
PkrI GCNGC 6 cut(s) 550, 777, 1267, 1890, 1927, 2059
PleI GAGTC 1 cut(s) 1723
PpsI GAGTC 1 cut(s) 1723
Ppu21I YACGTR 2 cut(s) 789, 1353
PpuMI RGGWCCY 1 cut(s) 377
PscI ACATGT 1 cut(s) 1735
PshBI ATTAAT 1 cut(s) 1523
Psp5II RGGWCCY 1 cut(s) 377
Psp6I CCWGG 3 cut(s) 873, 1018, 1809
PspEI GGTNACC 1 cut(s) 1904
PspFI CCCAGC 4 cut(s) 1105, 1558, 2122, 2140
PspGI CCWGG 3 cut(s) 873, 1018, 1809
PspN4I GGNNCC 5 cut(s) 378, 396, 2156, 2157, 2163
PspOMI GGGCCC 1 cut(s) 2154
PspPI GGNCC 6 cut(s) 103, 377, 877, 2072, 2154, 2155
PspPPI RGGWCCY 1 cut(s) 377
PstI CTGCAG 1 cut(s) 777
PsuI RGATCY 2 cut(s) 1483, 1679
PvuII CAGCTG 2 cut(s) 506, 1925
RsaI GTAC 3 cut(s) 1002, 1281, 2202
RsaNI GTAC 3 cut(s) 1001, 1280, 2201
RseI CAYNNNNRTG 2 cut(s) 255, 1422
SapI GCTCTTC 2 cut(s) 1187, 1219
SaqAI TTAA 7 cut(s) 129, 318, 485, 842, 1100, 1523, 1541
SatI GCNGC 6 cut(s) 549, 776, 1266, 1889, 1926, 2058
Sau3AI GATC 6 cut(s) 607, 1033, 1483, 1672, 1679, 1686
Sau96I GGNCC 6 cut(s) 103, 377, 877, 2072, 2154, 2155
SchI GAGTC 1 cut(s) 1724
ScrFI CCNGG 7 cut(s) 530, 875, 1020, 1811, 2019, 2020, 2159
SduI GDGCHC 2 cut(s) 1231, 2158
SfaNI GCATC 6 cut(s) 802, 1119, 1252, 1294, 1704, 2220
SfcI CTRYAG 1 cut(s) 773
Sfr274I CTCGAG 1 cut(s) 1712
SfuI TTCGAA 1 cut(s) 977
SinI GGWCC 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 1712
SmaI CCCGGG 1 cut(s) 2020
SmiMI CAYNNNNRTG 2 cut(s) 255, 1422
SmlI CTYRAG 2 cut(s) 65, 1712
SmoI CTYRAG 2 cut(s) 65, 1712
SnaBI TACGTA 2 cut(s) 789, 1353
SpeI ACTAGT 1 cut(s) 1504
SsiI CCGC 3 cut(s) 1235, 1731, 1934
SspI AATATT 2 cut(s) 1052, 2065
SspMI CTAG 6 cut(s) 621, 1319, 1505, 1854, 1929, 2151
StyD4I CCNGG 7 cut(s) 528, 873, 1018, 1809, 2017, 2018, 2157
TaaI ACNGT 6 cut(s) 1219, 1272, 1569, 1788, 1977, 2186
TaiI ACGT 4 cut(s) 791, 1002, 1355, 1373
TaqI TCGA 4 cut(s) 134, 344, 977, 1713
TatI WGTACW 1 cut(s) 1279
TfiI GAWTC 8 cut(s) 341, 416, 602, 906, 944, 979, 1589, 1597
Tru1I TTAA 7 cut(s) 129, 318, 485, 842, 1100, 1523, 1541
Tru9I TTAA 7 cut(s) 129, 318, 485, 842, 1100, 1523, 1541
TscAI CASTG 7 cut(s) 190, 690, 718, 957, 1572, 2178, 2191
TseFI GTSAC 2 cut(s) 1904, 1977
TseI GCWGC 6 cut(s) 548, 775, 1265, 1888, 1925, 2057
Tsp45I GTSAC 2 cut(s) 1904, 1977
TspGWI ACGGA 1 cut(s) 599
TspMI CCCGGG 1 cut(s) 2018
TspRI CASTG 7 cut(s) 190, 690, 718, 957, 1572, 2178, 2191
Van91I CCANNNNNTGG 1 cut(s) 717
VpaK11BI GGWCC 1 cut(s) 377
VspI ATTAAT 1 cut(s) 1523
XagI CCTNNNNNAGG 1 cut(s) 185
XapI RAATTY 9 cut(s) 20, 574, 1012, 1068, 1096, 1463, 1550, 1611, 2013
XceI RCATGY 4 cut(s) 1007, 1241, 1427, 1739
XcmI CCANNNNNNNNNTGG 1 cut(s) 1238
XhoI CTCGAG 1 cut(s) 1712
XmaI CCCGGG 1 cut(s) 2018
XmiI GTMKAC 1 cut(s) 1656
XmnI GAANNNNTTC 1 cut(s) 1196
XspI CTAG 6 cut(s) 621, 1319, 1505, 1854, 1929, 2151
Zsp2I ATGCAT 1 cut(s) 1697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.