Rw3G003690

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000126
Physical Location & Seq
Forward (+)
3143908 .. 3148135
4228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003690.1

Sequence Viewer

Length: 2697 bp
ATGGAGAAGATAGCATCCGATTTGAAAGTATCGGAGTTGAAGCAGAGGAGTCTAGGCAAGGCGTACGAGACCTTGCATTCCCAAGCGTCTTCCATACTCGTTCTCAGCATTCAATGGAAGGACCTCCAGGAACACTTCGATTCGACCCGGAATGCTATCCGGACTCGTTTCGAAGAGCTTCAGGAACGAGAGAAGCAGTTGGAAGCCAAGGAGTCAAACTTGAAGTCGGAGATGGAATCCAAGAAGGACGAGTTGTTTGGAGTTGAGAAGCTGATTGATGAGAAAGTACAGGAGGTTATAGACATAAAGAGATCGATTCAAAAGCACAGTGAGGAGATTGAACGTGAAGAGAAGAGAGTCTTGCAAGTACGGAAATTGGTTATAGACAAGGAGAGGGAGTGTAGATTGATCCAAAGACGGATTCAAGAGAGTGGGAAGAAACTGAGTTGGGTTGAGAGAAGAACTGCAGAGAAATTGAATGAGGTTGAGTGTAAGGAGAAGGAACTGAACAAGTACCGTGAGGATGCTAAGTTGAAAGTGGAGCAGCTAAGTGTGATTGATGGGGCAATTGTGGAGCGTAACGAAATCATCGAGTCAAAAGAGGAGAAATTGAGTTGGATTGAGAAGAGTATTGAAGAGAAGTCCAAGTTGGCCAACTCTAAGGAGGAGGAAGTGAGAGCGATACAACGGTCGTTGAATGTATACCGTGAGAGTATTAAGTCAAAAGAGAGTGACTTAGATGCGATTCTTGAGTCGATTGAAGGGGAGAAGAAAGAATTTGATGTGAAAGAAGAGCAAATTAAAACGTTGCAGAGATTGATTGAGGGATGTGAGAAAGAGCTCAAATTGAAAGAGGAGATATTGAAGGAGGAAACACTGAAAGTGAAAGAATACAGTTTGCGAAATAGACAAATGGAGGAGTGGTCCTGCAGGCTTGAACTTAAAGAGAGGGAAGTTGAATTGAAAGAGAAACGATTAGAGTCAAAAATGGAGGAATTTAAAAAATGGGTTGAAGAACGAGGAAAGGATTTGAATTCACGCTCCTCGGAACTGAAAGTTAAAGAGTGTCAACTTGAACAGGCGGCCAAAGAGCTTGAGTTGACTAAGAAACAATGTGGTGTTGTTGCTCAAGTGAAGATTGGGCCATTGGAAAAGACTCCAGAGAACGATGCCATTGTTTCTTCATCTGCAAGTGATCAATCCAACATCAATATTACCGACGGTAGAAGTTTGCAGTTGTTCATGTATGAGCATATGAAGAGAAATGATTCAATAAGCAACAAGATCTCATCCTTTCTCCAACAAAAGGCATCAGAACCAGCAAAATTGGTTTTGGATGCAATGCAAGGGTTTTACACTTCAAATTTGACTGTCGAGAACGAGTTCGATTTGGTGCTTATTAGAAGGACATGCATTCTTTTGTTGGAAGAATTAAAGAGAGTCTCACCCCAAACTAATCCAGAGGTCAGAGAAGAAGCAATGAAGTTGGCCACTGATTGGAAGGCTAAGATGAAAGTGGGGAGTGAGAACTGGTTGGAGATTTTGGGTTTTTTGCGGCTTGTTACTACTTATGGATTGACCTCTGCTTATAATGAGAAGGAGCTTCACAGTCTTCTTGATATAGTTGCTCAGCATGAACAAGCAACAGAATTATCACGGGCCCTTGCTACCACAGATAAGGCACATGCAAATAGCAACATCTGTTCTCCTATGAAAATTGAAAAAACAAAATCTCCAGTAGCCCAAAATGAAGCAACCATCTCTTCTCCAAATCTTCAGCAAGCTGCCACCACTATTGACGCAAGGAACTTGCAGGGGTTTCAAAATGAGCAGTTTCGTGAAGCCTGTGATTCTGCTGATTCTGCTTGGAAAAGTATTATTTATGATCTTATTGGAAAGAAGAGACTGATTGCGGCTGTTGGGTTGATTTACACCATGAAGTTATTTGACAAGTTTCCCCCAGTACAAATCTTAAAAGAATATGTTGATAATGGAAAGAGGTACTGCAGGAATAGAACCAAGAGAAAGAAATCGCTGGATGAAAAGGATATGATTACAGACCAGCATATAGCAGATCTACGAGTTGTTATTCAATGTATCAAAGATTACAACCTCGAGTCTGAATGTCCACCTGAAGAAATTGAATCACAAATTGCTGCGCTTGAAATTGTTAAGGAGCGGAGACGTTTTGTGTCATCTTCTCTTGTTTTGAAGGTTGAACAACAGCAAAAGGTGCTGAAAAATGTTGAAGTACAAGAACAACAAGAGCAGAAAGCCTCCAAGGGTGAACAACAAGAACAGCAAAAAGAACAGAGAACCTCTGAGGCTAAACTACAAGAAAAAGTACAGCAGAAAGCCTCCAATAAGGTTGAAGAACAACAACAGCAACAAGAGAAAAACGCCTCCAAGGTTAAACATCAAGAACAACAAGAGCAGAAAGCCTTCAAAGTTAAACAATTGCAGAAAAAAGGGAATAAACGTCCAAACAGCACATTTGCCCCCTGGTTTGATCACCAACAGCAGTGTAAAAATAAATTTCAAAGAACATCTATATTAGCTGATAGACACCCGGACCACCCTGACTATCCGAATCCAAGTTCATCAACTTGGACACATGAAAGTAATGGATACCGTGAACAGTTTGGTACTGCCGTCAACAAGTATGAAGTTCATGCTAATTATGGACATCCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

898

Amino Acids

104.5

Weight (kDa)

6.81

Isoelectric Point (pI)

58.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 407 - 560 2.7e-32 Frigida-like protein
Frigida PF07899 624 - 728 1.1e-21 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1590
AccB7I CCANNNNNTGG 1 cut(s) 1497
AccBSI CCGCTC 1 cut(s) 2179
AccI GTMKAC 1 cut(s) 702
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 4 cut(s) 1082, 1555, 1913, 2179
AclI AACGTT 1 cut(s) 806
AclWI GGATC 1 cut(s) 403
AcoI YGGCCR 3 cut(s) 651, 1083, 1488
AcsI RAATTY 5 cut(s) 776, 995, 1033, 1363, 2534
AcuI CTGAAG 3 cut(s) 164, 1760, 2154
AfaI GTAC 9 cut(s) 65, 288, 369, 515, 1965, 2003, 2253, 2346, 2647
AfiI CCNNNNNNNGG 2 cut(s) 1306, 1497
AjnI CCWGG 2 cut(s) 126, 2501
AjuI GAANNNNNNNTTGG 4 cut(s) 405, 437, 632, 664
AluBI AGCT 8 cut(s) 178, 271, 547, 841, 1093, 1603, 1784, 2558
AluI AGCT 8 cut(s) 178, 271, 547, 841, 1093, 1603, 1784, 2558
Alw21I GWGCWC 1 cut(s) 843
Alw26I GTCTC 4 cut(s) 62, 1447, 1897, 2176
AlwI GGATC 1 cut(s) 403
Ama87I CYCGRG 1 cut(s) 2114
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 5 cut(s) 651, 1083, 1142, 1488, 1659
ApaI GGGCCC 1 cut(s) 1663
ApeKI GCWGC 3 cut(s) 544, 1784, 2156
ApoI RAATTY 5 cut(s) 776, 995, 1033, 1363, 2534
ArsI GACNNNNNNTTYG 4 cut(s) 209, 239, 241, 271
Asp700I GAANNNNTTC 4 cut(s) 177, 1267, 1382, 2441
AspLEI GCGC 1 cut(s) 2161
AspS9I GGNCC 6 cut(s) 121, 924, 1142, 1659, 1660, 2572
AsuC2I CCSGG 2 cut(s) 148, 2570
AsuHPI GGTGA 3 cut(s) 1437, 2297, 2504
AsuII TTCGAA 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 2114
AvaII GGWCC 3 cut(s) 121, 924, 2572
BaeGI GKGCMC 1 cut(s) 1663
BaeI ACNNNNGTAYC 4 cut(s) 674, 707, 2637, 2670
BalI TGGCCA 2 cut(s) 653, 1490
BanII GRGCYC 2 cut(s) 843, 1663
BbsI GAAGAC 2 cut(s) 81, 1604
Bbv12I GWGCWC 1 cut(s) 843
BbvI GCAGC 3 cut(s) 556, 1771, 2143
BccI CCATC 3 cut(s) 226, 554, 1766
BceAI ACGGC 1 cut(s) 2636
BciT130I CCWGG 2 cut(s) 128, 2503
BciVI GTATCC 1 cut(s) 2621
BclI TGATCA 2 cut(s) 1195, 2509
BcnI CCSGG 2 cut(s) 148, 2570
BcoDI GTCTC 4 cut(s) 62, 1447, 1897, 2176
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 3 cut(s) 465, 928, 2005
BfuI GTATCC 1 cut(s) 2621
BglII AGATCT 2 cut(s) 1284, 2074
BisI GCNGC 6 cut(s) 545, 1083, 1556, 1785, 1914, 2157
BlpI GCTNAGC 1 cut(s) 1629
BlsI GCNGC 6 cut(s) 546, 1084, 1557, 1786, 1915, 2158
Bme1390I CCNGG 4 cut(s) 128, 148, 2503, 2570
Bme18I GGWCC 3 cut(s) 121, 924, 2572
BmeT110I CYCGRG 1 cut(s) 2114
BmgT120I GGNCC 6 cut(s) 121, 924, 1142, 1659, 1660, 2572
BmiI GGNNCC 1 cut(s) 1661
BmrFI CCNGG 4 cut(s) 128, 148, 2503, 2570
BmrI ACTGGG 1 cut(s) 1955
BmsI GCATC 6 cut(s) 23, 514, 730, 1159, 1319, 1327
BmuI ACTGGG 1 cut(s) 1955
BpiI GAAGAC 2 cut(s) 81, 1604
BpmI CTGGAG 3 cut(s) 110, 1143, 1719
Bpu1102I GCTNAGC 1 cut(s) 1629
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 3 cut(s) 770, 1113, 1115
BpuMI CCSGG 2 cut(s) 148, 2570
Bsa29I ATCGAT 1 cut(s) 314
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 5 cut(s) 207, 1044, 2280, 2406, 2501
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 6 cut(s) 383, 413, 908, 938, 1717, 1747
Bsc4I CCNNNNNNNGG 2 cut(s) 1306, 1497
Bse1I ACTGG 3 cut(s) 1535, 1736, 1961
Bse3DI GCAATG 2 cut(s) 1347, 1485
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 2 cut(s) 128, 2503
BseCI ATCGAT 1 cut(s) 314
BseDI CCNNGG 5 cut(s) 207, 1044, 2280, 2406, 2501
BseGI GGATG 7 cut(s) 14, 529, 833, 1289, 1342, 2044, 2686
BseLI CCNNNNNNNGG 2 cut(s) 1306, 1497
BseMI GCAATG 2 cut(s) 1347, 1485
BseMII CTCAG 4 cut(s) 118, 434, 1643, 2313
BseNI ACTGG 3 cut(s) 1535, 1736, 1961
BseRI GAGGAG 7 cut(s) 61, 347, 617, 680, 869, 932, 1033
BseSI GKGCMC 1 cut(s) 1663
BseXI GCAGC 3 cut(s) 556, 1771, 2143
Bsh1285I CGRYCG 1 cut(s) 692
BshFI GGCC 5 cut(s) 653, 1085, 1144, 1490, 1661
BshVI ATCGAT 1 cut(s) 314
BsiEI CGRYCG 1 cut(s) 692
BsiHKAI GWGCWC 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 2114
BsiSI CCGG 3 cut(s) 148, 160, 2570
BsiWI CGTACG 1 cut(s) 63
BslI CCNNNNNNNGG 2 cut(s) 1306, 1497
BsmAI GTCTC 4 cut(s) 62, 1447, 1897, 2176
BsmBI CGTCTC 1 cut(s) 2176
BsmI GAATGC 4 cut(s) 76, 108, 157, 1413
BsnI GGCC 5 cut(s) 653, 1085, 1144, 1490, 1661
Bso31I GGTCTC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 2114
Bsp119I TTCGAA 1 cut(s) 171
Bsp120I GGGCCC 1 cut(s) 1659
Bsp1286I GDGCHC 2 cut(s) 843, 1663
Bsp13I TCCGGA 1 cut(s) 159
Bsp143I GATC 7 cut(s) 311, 408, 1195, 1284, 1885, 2074, 2509
Bsp1720I GCTNAGC 1 cut(s) 1629
BspACI CCGC 4 cut(s) 1082, 1555, 1913, 2179
BspANI GGCC 5 cut(s) 653, 1085, 1144, 1490, 1661
BspCNI CTCAG 4 cut(s) 117, 435, 1642, 2314
BspDI ATCGAT 1 cut(s) 314
BspEI TCCGGA 1 cut(s) 159
BspLI GGNNCC 1 cut(s) 1661
BspMAI CTGCAG 3 cut(s) 469, 932, 2009
BspPI GGATC 1 cut(s) 403
BspQI GCTCTTC 2 cut(s) 168, 786
BspT104I TTCGAA 1 cut(s) 171
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 2179
BsrDI GCAATG 2 cut(s) 1347, 1485
BsrI ACTGG 3 cut(s) 1535, 1736, 1961
BssECI CCNNGG 5 cut(s) 207, 1044, 2280, 2406, 2501
BssMI GATC 7 cut(s) 311, 408, 1195, 1284, 1885, 2074, 2509
BssNAI GTATAC 1 cut(s) 703
BssT1I CCWWGG 3 cut(s) 207, 2280, 2406
Bst1107I GTATAC 1 cut(s) 703
Bst2UI CCWGG 2 cut(s) 128, 2503
Bst6I CTCTTC 9 cut(s) 168, 342, 347, 620, 630, 786, 1253, 1768, 1895
BstAPI GCANNNNNTGC 1 cut(s) 2233
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 932, 1782
BstF5I GGATG 7 cut(s) 14, 529, 833, 1289, 1342, 2044, 2686
BstHHI GCGC 1 cut(s) 2161
BstKTI GATC 7 cut(s) 314, 411, 1198, 1287, 1888, 2077, 2512
BstMAI GTCTC 4 cut(s) 62, 1447, 1897, 2176
BstMBI GATC 7 cut(s) 311, 408, 1195, 1284, 1885, 2074, 2509
BstMCI CGRYCG 1 cut(s) 692
BstMWI GCNNNNNNNGC 2 cut(s) 1862, 2233
BstNI CCWGG 2 cut(s) 128, 2503
BstNSI RCATGY 2 cut(s) 1413, 1688
BstSCI CCNGG 4 cut(s) 126, 146, 2501, 2568
BstSFI CTRYAG 3 cut(s) 465, 928, 2005
BstSLI GKGCMC 1 cut(s) 1663
BstV1I GCAGC 3 cut(s) 556, 1771, 2143
BstV2I GAAGAC 2 cut(s) 81, 1604
BstX2I RGATCY 2 cut(s) 1284, 2074
BstYI RGATCY 2 cut(s) 1284, 2074
BstZ17I GTATAC 1 cut(s) 703
Bsu15I ATCGAT 1 cut(s) 314
BsuI GTATCC 1 cut(s) 2621
BsuRI GGCC 5 cut(s) 653, 1085, 1144, 1490, 1661
BsuTUI ATCGAT 1 cut(s) 314
BtsCI GGATG 7 cut(s) 14, 529, 833, 1289, 1342, 2044, 2686
BtsI GCAGTG 1 cut(s) 2528
BtsIMutI CAGTG 4 cut(s) 334, 875, 1491, 2528
Cac8I GCNNGC 2 cut(s) 932, 1782
CfoI GCGC 1 cut(s) 2161
Cfr13I GGNCC 6 cut(s) 121, 924, 1142, 1659, 1660, 2572
ClaI ATCGAT 1 cut(s) 314
CseI GACGC 2 cut(s) 75, 1808
Csp6I GTAC 9 cut(s) 64, 287, 368, 514, 1964, 2002, 2252, 2345, 2646
CspCI CAANNNNNGTGG 2 cut(s) 1777, 1812
CviAII CATG 7 cut(s) 1243, 1410, 1634, 1685, 1936, 2615, 2672
CviQI GTAC 9 cut(s) 64, 287, 368, 514, 1964, 2002, 2252, 2345, 2646
DpnI GATC 7 cut(s) 313, 410, 1197, 1286, 1887, 2076, 2511
DpnII GATC 7 cut(s) 311, 408, 1195, 1284, 1885, 2074, 2509
DraI TTTAAA 1 cut(s) 1000
EaeI YGGCCR 3 cut(s) 651, 1083, 1488
Eam1104I CTCTTC 9 cut(s) 168, 342, 347, 620, 630, 786, 1253, 1768, 1895
EarI CTCTTC 9 cut(s) 168, 342, 347, 620, 630, 786, 1253, 1768, 1895
Ecl136II GAGCTC 1 cut(s) 841
Eco130I CCWWGG 3 cut(s) 207, 2280, 2406
Eco24I GRGCYC 2 cut(s) 843, 1663
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 3 cut(s) 121, 924, 2572
Eco53kI GAGCTC 1 cut(s) 841
Eco57I CTGAAG 3 cut(s) 164, 1760, 2154
Eco88I CYCGRG 1 cut(s) 2114
EcoICRI GAGCTC 1 cut(s) 841
EcoO109I RGGNCCY 2 cut(s) 121, 1660
EcoRI GAATTC 1 cut(s) 1033
EcoRII CCWGG 2 cut(s) 126, 2501
EcoT14I CCWWGG 3 cut(s) 207, 2280, 2406
EcoT22I ATGCAT 1 cut(s) 1415
EcoT38I GRGCYC 2 cut(s) 843, 1663
ErhI CCWWGG 3 cut(s) 207, 2280, 2406
Esp3I CGTCTC 1 cut(s) 2176
FaeI CATG 7 cut(s) 1246, 1413, 1637, 1688, 1939, 2618, 2675
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FatI CATG 7 cut(s) 1242, 1409, 1633, 1684, 1935, 2614, 2671
FauNDI CATATG 1 cut(s) 1254
FbaI TGATCA 2 cut(s) 1195, 2509
FblI GTMKAC 1 cut(s) 702
Fnu4HI GCNGC 6 cut(s) 545, 1083, 1556, 1785, 1914, 2157
FokI GGATG 6 cut(s) 536, 840, 1276, 1349, 2051, 2673
FriOI GRGCYC 2 cut(s) 843, 1663
Fsp4HI GCNGC 6 cut(s) 545, 1083, 1556, 1785, 1914, 2157
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 2160
GluI GCNGC 6 cut(s) 545, 1083, 1556, 1785, 1914, 2157
GsuI CTGGAG 3 cut(s) 110, 1143, 1719
HaeIII GGCC 5 cut(s) 653, 1085, 1144, 1490, 1661
HapII CCGG 3 cut(s) 148, 160, 2570
HgaI GACGC 2 cut(s) 75, 1808
HhaI GCGC 1 cut(s) 2161
Hin1II CATG 7 cut(s) 1246, 1413, 1637, 1688, 1939, 2618, 2675
Hin6I GCGC 1 cut(s) 2159
HinP1I GCGC 1 cut(s) 2159
HincII GTYRAC 3 cut(s) 1070, 1101, 2656
HindII GTYRAC 3 cut(s) 1070, 1101, 2656
HpaII CCGG 3 cut(s) 148, 160, 2570
HphI GGTGA 3 cut(s) 1437, 2297, 2504
Hpy166II GTNNAC 7 cut(s) 703, 1070, 1101, 2129, 2288, 2636, 2656
Hpy188I TCNGA 9 cut(s) 19, 34, 229, 1048, 1315, 1469, 2122, 2323, 2589
Hpy8I GTNNAC 7 cut(s) 703, 1070, 1101, 2129, 2288, 2636, 2656
Hpy99I CGWCG 1 cut(s) 1223
HpyCH4IV ACGT 4 cut(s) 343, 806, 2185, 2479
HpyF10VI GCNNNNNNNGC 2 cut(s) 1862, 2233
HpySE526I ACGT 4 cut(s) 343, 806, 2185, 2479
Hsp92II CATG 7 cut(s) 1246, 1413, 1637, 1688, 1939, 2618, 2675
HspAI GCGC 1 cut(s) 2159
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 2 cut(s) 1195, 2509
Kzo9I GATC 7 cut(s) 311, 408, 1195, 1284, 1885, 2074, 2509
LguI GCTCTTC 2 cut(s) 168, 786
LmnI GCTCC 5 cut(s) 541, 574, 1046, 1600, 2176
Lsp1109I GCAGC 3 cut(s) 556, 1771, 2143
LweI GCATC 6 cut(s) 23, 514, 730, 1159, 1319, 1327
MaeI CTAG 1 cut(s) 53
MaeII ACGT 4 cut(s) 343, 806, 2185, 2479
MaeIII GTNAC 3 cut(s) 578, 731, 1561
MalI GATC 7 cut(s) 313, 410, 1197, 1286, 1887, 2076, 2511
MbiI CCGCTC 1 cut(s) 2179
MboI GATC 7 cut(s) 311, 408, 1195, 1284, 1885, 2074, 2509
MfeI CAATTG 2 cut(s) 567, 2456
MflI RGATCY 2 cut(s) 1284, 2074
MhlI GDGCHC 2 cut(s) 843, 1663
MlsI TGGCCA 2 cut(s) 653, 1490
MluNI TGGCCA 2 cut(s) 653, 1490
MmeI TCCRAC 7 cut(s) 180, 207, 596, 1227, 1324, 1404, 1515
Mox20I TGGCCA 2 cut(s) 653, 1490
Mph1103I ATGCAT 1 cut(s) 1415
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 4 cut(s) 177, 1267, 1382, 2441
MscI TGGCCA 2 cut(s) 653, 1490
Msp20I TGGCCA 2 cut(s) 653, 1490
MspI CCGG 3 cut(s) 148, 160, 2570
MspR9I CCNGG 4 cut(s) 128, 148, 2503, 2570
MunI CAATTG 2 cut(s) 567, 2456
Mva1269I GAATGC 4 cut(s) 76, 108, 157, 1413
MvaI CCWGG 2 cut(s) 128, 2503
MwoI GCNNNNNNNGC 2 cut(s) 1862, 2233
NciI CCSGG 2 cut(s) 148, 2570
NdeI CATATG 1 cut(s) 1254
NdeII GATC 7 cut(s) 311, 408, 1195, 1284, 1885, 2074, 2509
NlaIII CATG 7 cut(s) 1246, 1413, 1637, 1688, 1939, 2618, 2675
NlaIV GGNNCC 1 cut(s) 1661
NmuCI GTSAC 1 cut(s) 731
NsiI ATGCAT 1 cut(s) 1415
NspI RCATGY 2 cut(s) 1413, 1688
NspV TTCGAA 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 2114
PciSI GCTCTTC 2 cut(s) 168, 786
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PctI GAATGC 4 cut(s) 76, 108, 157, 1413
PdmI GAANNNNTTC 4 cut(s) 177, 1267, 1382, 2441
Pfl23II CGTACG 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 1497
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 6 cut(s) 546, 1084, 1557, 1786, 1915, 2158
PpuMI RGGWCCY 1 cut(s) 121
PsiI TTATAA 1 cut(s) 1590
Psp124BI GAGCTC 1 cut(s) 843
Psp1406I AACGTT 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 2 cut(s) 126, 2501
PspGI CCWGG 2 cut(s) 126, 2501
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 1661
PspOMI GGGCCC 1 cut(s) 1659
PspPI GGNCC 6 cut(s) 121, 924, 1142, 1659, 1660, 2572
PspPPI RGGWCCY 1 cut(s) 121
PspXI VCTCGAGB 1 cut(s) 2114
PstI CTGCAG 3 cut(s) 469, 932, 2009
PsuI RGATCY 2 cut(s) 1284, 2074
RsaI GTAC 9 cut(s) 65, 288, 369, 515, 1965, 2003, 2253, 2346, 2647
RsaNI GTAC 9 cut(s) 64, 287, 368, 514, 1964, 2002, 2252, 2345, 2646
SacI GAGCTC 1 cut(s) 843
SapI GCTCTTC 2 cut(s) 168, 786
SatI GCNGC 6 cut(s) 545, 1083, 1556, 1785, 1914, 2157
Sau3AI GATC 7 cut(s) 311, 408, 1195, 1284, 1885, 2074, 2509
Sau96I GGNCC 6 cut(s) 121, 924, 1142, 1659, 1660, 2572
SbfI CCTGCAGG 1 cut(s) 932
ScrFI CCNGG 4 cut(s) 128, 148, 2503, 2570
SdaI CCTGCAGG 1 cut(s) 932
SduI GDGCHC 2 cut(s) 843, 1663
SfaNI GCATC 6 cut(s) 23, 514, 730, 1159, 1319, 1327
SfcI CTRYAG 3 cut(s) 465, 928, 2005
Sfr274I CTCGAG 1 cut(s) 2114
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 3 cut(s) 121, 924, 2572
SlaI CTCGAG 1 cut(s) 2114
SmlI CTYRAG 4 cut(s) 749, 1094, 1128, 2114
SmoI CTYRAG 4 cut(s) 749, 1094, 1128, 2114
Sse8387I CCTGCAGG 1 cut(s) 932
SsiI CCGC 4 cut(s) 1082, 1555, 1913, 2179
SspI AATATT 1 cut(s) 1213
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 1 cut(s) 843
StyD4I CCNGG 4 cut(s) 126, 146, 2501, 2568
StyI CCWWGG 3 cut(s) 207, 2280, 2406
TaiI ACGT 4 cut(s) 346, 809, 2188, 2482
TaqI TCGA 9 cut(s) 138, 143, 171, 314, 591, 755, 1374, 1386, 2115
TatI WGTACW 4 cut(s) 286, 1963, 2251, 2344
TauI GCSGC 3 cut(s) 1085, 1558, 1916
TscAI CASTG 4 cut(s) 334, 882, 1498, 2528
TseFI GTSAC 1 cut(s) 731
TseI GCWGC 3 cut(s) 544, 1784, 2156
Tsp45I GTSAC 1 cut(s) 731
TspGWI ACGGA 2 cut(s) 385, 433
TspRI CASTG 4 cut(s) 334, 882, 1498, 2528
Van91I CCANNNNNTGG 1 cut(s) 1497
VpaK11BI GGWCC 3 cut(s) 121, 924, 2572
XapI RAATTY 5 cut(s) 776, 995, 1033, 1363, 2534
XceI RCATGY 2 cut(s) 1413, 1688
XhoI CTCGAG 1 cut(s) 2114
XmiI GTMKAC 1 cut(s) 702
XmnI GAANNNNTTC 4 cut(s) 177, 1267, 1382, 2441
XspI CTAG 1 cut(s) 53
Zsp2I ATGCAT 1 cut(s) 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.