MD12G1229200.v1.1

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000126
Physical Location & Seq
Reverse (-)
30463318 .. 30468805
5488 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1229200.v1.1.491

Sequence Viewer

Length: 3771 bp
ATGGAGAAGATAGAGGCAGAATTGAAGGAGGCGGAGACGAAGCAGAGGAGTCTGAGCAAGGCGTACGAGTGCGTACACGCGCAGGCGACTGCGCTTATTATCTTCGCCGTGCAATGGAAGGACCTGGAGGAGCACTTTGAGTCGACTCGGTACTCGCTCGAGTCGCGATTCCGGGAACTCGCCTACCGCGAGACGGCGGTCCGGGTCCGGGACGAGGAGTTGGCGGAGAAGGAGATGAAGTTCTACACGGAAGTTGAATCGAAAGCGGACGAGTTGGGGAGGATTCAGATTCTGATCGACGAGAAGGCTGAGGAGGCTATACAGAGTAAGAACCACTTGCAATCTCTCCAGTCTTTGATTCTGGAACATAACGATGAGGTTATGGTGCAAGAGAAGAGGTTGATGGAGGTTGAGAGTTTCGTTGGGGAGAAGAAGAGGGAGTGCGATTCGATTGAGAGACGGGTTAAGGAGAGGATGAAGAAGTTGAATTGGGTTGAGAGAATGGTGGAGGAGAAGTCGAAATTGGCTGAGTTGAAGGGGGAGAAATTGAGGGGGTTTGAAGAGGCGTTGGAGAAGTGTGTAGAGAAGACTGAATTGAAGGAGAGGGAGTTGAATGAGATTATCGGGTCGATTGAGGAGCGCAAGGAGGAGTTCAGTTGGATAGGAGAGCAGATTTTGGAGGCGGAAAAGCTGATTAAGGTGCAAGAGGAGCAGTTGGATTTGAAAGAAGAGAAATTTAAGGAGGTGCAAAAGGCGGTTGAAGAGTGTGAGAAAGAGTTGAAATTAAGAGCGGAGCGGATTAAGGAGGCTGAAAGGGCGGTTGAAGAATGTGACAAGGAGTTGAAAATGAAGAAGGAGAAACTTAGTGCGATGGAGAAATCGTTAGTGGAGTGCTCGAATGCCATGGAATCAAGGGAGACAAAGATTAGGGAAATGGACTCGAAAAAAGATAAAGATTTTTGCTTGCGTGAGAGATCATTGGAGGAGTGGTCCTGTAAGCTTGAGTTTAAAGAGAGGGAACTTGTATTGAAAGAGAGAAATGTCGAGTTAAAAGCTGGGGAACTCCGAAAGTCTAAAGAAGAAGGCGAAAAGTATTTGGAATCTCTCTCACAGGGACTTAAGGAGAAAGAGAACCAACTTCAAGGCCTTGACAAAGAGCTCAGATTGAAGCAGAAAGAACTTGATTTGATCAAAAAATCCACTCAAGAACGCGCCAAAGACCTGGAATTGAAAGAGAGGCAGCTTGAAGATCAGGCCAAAGAGTGTTCGTTGAAGCAGATAGAATTTAATTCGATCAAAAAGTTCACTGAAGAACGCAGCCAAAACCTTGAACTGAAAGAGAGGCAACTTGAAGACCAAGCCAAAGAGCTTGAATTGAAGCAGAAAGAATTTGATTCGATAAAAAAATTCTCAGAAGAACGCAGCCAAAAACTTAACCTGAAAGAGAGGCAACTTGAAGACCGGGCCAAAGAGCTTGAATTGAAGCATAAAGAGTTTGATTCGTTAAAAAAATTCAGCGAGGAACACACCCAAAACCTGAAAGCGAAAGAAAATACTAGTATTTTTCTTTCTCAAGTGAAAATTGAGCAATTGGAACAAATCCCTGCCAACAATGCTGTTGTTCCTTCTCTCACAAGTAACCAGTCCAGCGTCAATATGGATGGTAGAGGCTCGCTGTTGTTCATAAATGAGCATTTGAAGAACTATGTTCTAGTGGGTAGTGAAATCTCAGCTGTTCTTCAGGTGTCACCAGACCCGGCAAAACTGGTTTTGGATTCTATGCAAGGGTTTTACCATTCAAATTCAAGGGCGGACAAAAGCGGGTGTGGTTTTGATTTGAGCATTACCAGGGTGAGTTGTATCCTTTTGTTGGAGACTTTAAAGAGTATCTCCCCCAAAATTAACCCTGAGGTGAAAGAAGAAGCAATTAAGTTGGCAGGTGATTGGAAGGTTAAAATGACGACTGGGACGGAGAATTGCTTGGATGTTTTGTGTTTTCTGCGGTTTGTTAGTACATACGGAATCACCTCTTTTTATGATGCGATTCAAAGTCTTTGTGCGATCGTTGCTGAGGATGAACACACAACTGAATTATCCTGTGCCCTTGGTATCAGTGATAAGGCACCCGCCAAAACTGAGACACCAGAATCTTTACTGGCAAAAAATGCTGGGACCTTTTCTTCTCCGAATCTTCAACTATCTGCCACCACAGATGCTAGTCATTTGCAGGGGGTTTTAAATGAGATTTTCAGTAGGGATCGTCTGTTGCAGAATGAAACTTGGGCTACTTTTCAAATGTCATCAGATCCAGAAAAGTTTGTGATGGATGTGATGCAAACTTCTTTTGCTAAATACTGCACAGTAGAAGATGTTGGTTTTAGAGAAACTGTCATGTCGAAGTGCATTTCACTACTCGACAAGCTAATGAGAATTAAACCACATGTTGGACCTCATGTTAACAAAGATGCCCTAAAGCTAGCAGTCCACTGGAAATCAAAAATTGGTGCAGGCACCCAAGCCTTCGAGCTTTTGGGTTTTTTGCAGTTCATCGCTACATATGGATTGCTTTCTATGTTTAATAAAGAGATTTTAGTGTTTCTTGGGAGGATTTCTCAGCAGAAGCAGGCGCTAGAAGCATGTCAGACACTTGGTTTAGCTGATAAGATCCCTGATTTCATTCGGAATCTTATTGAAAAGAAGCAACTAATTGAAGCTGTTCAATTGATTTGCCCCTTCAAGTTAATTGACAAGTTTCACCCAGTACCTCTCTTGAAAGAATTTGTGGAGAATGCAAAGAGGTTGTGCATACAAAATAGCAAGAGAAGTAAATCACTTGATGAAAAGGATGAATGTATAAACAACCAGATTGCTGATCTTAGAGTTGTGATTCAATGTATCAAAGGTTGCAATCTCGAGTTCGAGTACCCTTCCAGGTTCATTGAAACACGAATAGCTTTTCTGGAAAAACTAAAGGAGGATCGGAGACGTCCCAAGACATCTCCTCCCTCCAAGGTTGAACAACACAATCAGAAAAATTCTCCGGTCTCCAAGGTTGAACAACAAGAGCAGAAAAAGGCTAGTGCCTCCAAAGTTGAGGGACAAGAGCAGAAAAATTCGCATGCCCCTAGGGTTGAAGGACAAGAGCAGAAAAAGTCTGCTGCCTCCAAAGGTGCGGGGCAAGAGCAAAAAAAATCTCCTTCCTCTAGGGTTGAGGGACAAGAGCATATCGAATCTCTTGCCTCCAATGTCAAACAAGGGGCAGCAAAGCCCCTTGCTTCCATTGTTGAGGGACGAGAGCAGACAATATCTGTTCCCTCCAAGGTTGAACCAGAACACCACAGAAGCGGTCAGAAAGTTGAGGGACAAGAGCAGAAAAAGTCTCACGCCTCTAGGGTTGAAGGACAAGAGCAGAAAAAATCCGCTGCCTCCAAAGGTGCGGCGCAAGAGCAAAAGAAATCTCCATCCTCCAATGTCGAACAAGGGGCAGAAAAGCCTCCTGCTTCCACCGTTGAACAAGAACAACACAGAAGAGGTCAGAAACGGAGACCCAACACTTTGCCTCGTCAGTTTCAACCACAGCAACAGCGTCAAAGCAAGTATCATCGGACATCTGAATCAATCGGCCCAGGGTATCGGAAGCCATCTTTGTGGCATGAAAACCATAGACACCGTGGTCAGTTCGGTAGGGATGCCAACAGCTATGAAATTGGTGCTAATGCTGGTACCGGTTTTACTGCCATCCCGAATCCTCGTCCTTGTGTTCCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida

Protein Analysis

1257

Amino Acids

143.98

Weight (kDa)

6.76

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 548 - 709 9.8e-31 Frigida-like protein
Frigida PF07899 732 - 996 1.6e-55 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1928
AasI GACNNNNNNGTC 1 cut(s) 3672
AatII GACGTC 1 cut(s) 2989
Acc36I ACCTGC 1 cut(s) 1928
Acc65I GGTACC 1 cut(s) 3722
AccB1I GGYRCC 3 cut(s) 2122, 2510, 3722
AccB7I CCANNNNNTGG 1 cut(s) 2444
AccBSI CCGCTC 2 cut(s) 791, 796
AccI GTMKAC 1 cut(s) 143
AccII CGCG 4 cut(s) 80, 166, 189, 1212
AclWI GGATC 4 cut(s) 2265, 2300, 2659, 2985
AcsI RAATTY 9 cut(s) 734, 1283, 1388, 1406, 1511, 1801, 2777, 3034, 3112
AcuI CTGAAG 2 cut(s) 1329, 1724
AcyI GRCGYC 1 cut(s) 2986
AfaI GTAC 7 cut(s) 65, 75, 152, 2014, 2763, 2924, 3724
AfiI CCNNNNNNNGG 6 cut(s) 114, 193, 208, 214, 1870, 2444
AflII CTTAAG 1 cut(s) 1118
AflIII ACRYGT 1 cut(s) 2440
AgeI ACCGGT 1 cut(s) 3725
AhlI ACTAGT 1 cut(s) 1556
AjnI CCWGG 5 cut(s) 123, 1221, 1847, 2930, 3625
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AloI GAACNNNNNNTCC 2 cut(s) 224, 256
Alw21I GWGCWC 3 cut(s) 135, 896, 1161
AlwI GGATC 4 cut(s) 2265, 2300, 2659, 2985
AlwNI CAGNNNCTG 1 cut(s) 292
Ama87I CYCGRG 2 cut(s) 158, 2912
AoxI GGCC 4 cut(s) 1144, 1254, 1464, 3622
ApeKI GCWGC 6 cut(s) 1240, 1317, 1422, 3158, 3260, 3422
ApoI RAATTY 9 cut(s) 734, 1283, 1388, 1406, 1511, 1801, 2777, 3034, 3112
AsiGI ACCGGT 1 cut(s) 3725
Asp700I GAANNNNTTC 1 cut(s) 2715
Asp718I GGTACC 1 cut(s) 3722
AspA2I CCTAGG 1 cut(s) 3125
AspLEI GCGC 6 cut(s) 82, 94, 642, 1214, 2629, 3442
AspS9I GGNCC 8 cut(s) 121, 199, 205, 990, 1464, 2172, 2447, 3623
AsuC2I CCSGG 5 cut(s) 173, 203, 209, 1463, 1757
AsuHPI GGTGA 6 cut(s) 1740, 1864, 1924, 1952, 2017, 2747
AsuNHI GCTAGC 1 cut(s) 2476
AvaI CYCGRG 2 cut(s) 158, 2912
AvaII GGWCC 6 cut(s) 121, 199, 205, 990, 2172, 2447
AvrII CCTAGG 1 cut(s) 3125
AxyI CCTNAGG 1 cut(s) 1908
BaeGI GKGCMC 1 cut(s) 2104
BanI GGYRCC 3 cut(s) 2122, 2510, 3722
BanII GRGCYC 1 cut(s) 1161
BbsI GAAGAC 3 cut(s) 593, 1359, 1464
Bbv12I GWGCWC 3 cut(s) 135, 896, 1161
BbvCI CCTCAGC 2 cut(s) 309, 2070
BbvI GCAGC 6 cut(s) 1252, 1329, 1434, 3145, 3272, 3409
BccI CCATC 7 cut(s) 397, 865, 1655, 2317, 3469, 3649, 3746
BceAI ACGGC 2 cut(s) 92, 210
BcgI CGANNNNNNTGC 2 cut(s) 3218, 3252
BciT130I CCWGG 5 cut(s) 125, 1223, 1849, 2932, 3627
BciVI GTATCC 1 cut(s) 1871
BclI TGATCA 1 cut(s) 1188
BcnI CCSGG 5 cut(s) 173, 203, 209, 1463, 1757
BcuI ACTAGT 1 cut(s) 1556
BfaI CTAG 9 cut(s) 1557, 1712, 2217, 2477, 2630, 3078, 3126, 3204, 3390
BfoI RGCGCY 1 cut(s) 2630
BfrI CTTAAG 1 cut(s) 1118
BfuAI ACCTGC 1 cut(s) 1928
BfuI GTATCC 1 cut(s) 1871
BisI GCNGC 7 cut(s) 1241, 1318, 1423, 3159, 3261, 3423, 3438
BlnI CCTAGG 1 cut(s) 3125
BlsI GCNGC 7 cut(s) 1242, 1319, 1424, 3160, 3262, 3424, 3439
Bme18I GGWCC 6 cut(s) 121, 199, 205, 990, 2172, 2447
BmeT110I CYCGRG 2 cut(s) 158, 2912
BmgT120I GGNCC 8 cut(s) 121, 199, 205, 990, 1464, 2172, 2447, 3623
BmiI GGNNCC 5 cut(s) 206, 2124, 2173, 2512, 3724
BmrI ACTGGG 2 cut(s) 1974, 2753
BmsI GCATC 5 cut(s) 2029, 2203, 2322, 2455, 3679
BmtI GCTAGC 1 cut(s) 2480
BmuI ACTGGG 2 cut(s) 1974, 2753
BoxI GACNNNNGTC 1 cut(s) 197
BpiI GAAGAC 3 cut(s) 593, 1359, 1464
BplI GAGNNNNNCTC 2 cut(s) 2596, 2628
BpmI CTGGAG 2 cut(s) 146, 332
Bpu10I CCTNAGC 2 cut(s) 309, 2070
BpuEI CTTGAG 3 cut(s) 1022, 1188, 1557
BpuMI CCSGG 5 cut(s) 173, 203, 209, 1463, 1757
BsaBI GATNNNNATC 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 2986
BsaI GGTCTC 2 cut(s) 3049, 3538
BsaWI WCCGGW 2 cut(s) 3040, 3725
Bsc4I CCNNNNNNNGG 6 cut(s) 114, 193, 208, 214, 1870, 2444
Bse118I RCCGGY 1 cut(s) 3725
Bse1I ACTGG 7 cut(s) 349, 1642, 1770, 1969, 2160, 2492, 2759
Bse21I CCTNAGG 1 cut(s) 1908
Bse3DI GCAATG 1 cut(s) 119
Bse8I GATNNNNATC 1 cut(s) 293
BseBI CCWGG 5 cut(s) 125, 1223, 1849, 2932, 3627
BseGI GGATG 9 cut(s) 480, 1666, 1990, 2080, 2332, 2851, 3461, 3694, 3738
BseJI GATNNNNATC 1 cut(s) 293
BseLI CCNNNNNNNGG 6 cut(s) 114, 193, 208, 214, 1870, 2444
BseMI GCAATG 1 cut(s) 119
BseNI ACTGG 7 cut(s) 349, 1642, 1770, 1969, 2160, 2492, 2759
BseSI GKGCMC 1 cut(s) 2104
BseXI GCAGC 6 cut(s) 1252, 1329, 1434, 3145, 3272, 3409
BseYI CCCAGC 2 cut(s) 1055, 2168
BsgI GTGCAG 2 cut(s) 2341, 2526
Bsh1236I CGCG 4 cut(s) 80, 166, 189, 1212
Bsh1285I CGRYCG 1 cut(s) 2064
BshFI GGCC 4 cut(s) 1146, 1256, 1466, 3624
BshNI GGYRCC 3 cut(s) 2122, 2510, 3722
BshTI ACCGGT 1 cut(s) 3725
BsiEI CGRYCG 1 cut(s) 2064
BsiHKAI GWGCWC 3 cut(s) 135, 896, 1161
BsiHKCI CYCGRG 2 cut(s) 158, 2912
BsiSI CCGG 7 cut(s) 172, 202, 208, 1462, 1757, 3041, 3726
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 9 cut(s) 224, 1128, 1981, 2185, 2973, 3111, 3228, 3303, 3375
BslI CCNNNNNNNGG 6 cut(s) 114, 193, 208, 214, 1870, 2444
BsmBI CGTCTC 4 cut(s) 29, 185, 451, 2977
BsmFI GGGAC 9 cut(s) 224, 1128, 1981, 2185, 2973, 3111, 3228, 3303, 3375
BsmI GAATGC 2 cut(s) 904, 2794
BsnI GGCC 4 cut(s) 1146, 1256, 1466, 3624
Bso31I GGTCTC 2 cut(s) 3049, 3538
BsoBI CYCGRG 2 cut(s) 158, 2912
Bsp1286I GDGCHC 4 cut(s) 135, 896, 1161, 2104
Bsp19I CCATGG 1 cut(s) 903
Bsp68I TCGCGA 1 cut(s) 166
BspANI GGCC 4 cut(s) 1146, 1256, 1466, 3624
BspFNI CGCG 4 cut(s) 80, 166, 189, 1212
BspLI GGNNCC 5 cut(s) 206, 2124, 2173, 2512, 3724
BspMI ACCTGC 1 cut(s) 1928
BspOI GCTAGC 1 cut(s) 2480
BspPI GGATC 4 cut(s) 2265, 2300, 2659, 2985
BspT107I GGYRCC 3 cut(s) 2122, 2510, 3722
BspTI CTTAAG 1 cut(s) 1118
BspTNI GGTCTC 2 cut(s) 3049, 3538
BsrBI CCGCTC 2 cut(s) 791, 796
BsrDI GCAATG 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 3725
BsrI ACTGG 7 cut(s) 349, 1642, 1770, 1969, 2160, 2492, 2759
BssAI RCCGGY 1 cut(s) 3725
BssNI GRCGYC 1 cut(s) 2986
BssT1I CCWWGG 6 cut(s) 903, 2104, 3009, 3048, 3125, 3318
Bst2UI CCWGG 5 cut(s) 125, 1223, 1849, 2932, 3627
Bst4CI ACNGT 4 cut(s) 2362, 2389, 3508, 3671
Bst6I CTCTTC 6 cut(s) 389, 428, 555, 723, 756, 3523
BstACI GRCGYC 1 cut(s) 2986
BstAFI CTTAAG 1 cut(s) 1118
BstAPI GCANNNNNTGC 1 cut(s) 2165
BstC8I GCNNGC 7 cut(s) 84, 965, 1673, 2478, 2509, 2625, 3120
BstDSI CCRYGG 2 cut(s) 903, 3670
BstF5I GGATG 9 cut(s) 480, 1666, 1990, 2080, 2332, 2851, 3461, 3694, 3738
BstFNI CGCG 4 cut(s) 80, 166, 189, 1212
BstH2I RGCGCY 1 cut(s) 2630
BstHHI GCGC 6 cut(s) 82, 94, 642, 1214, 2629, 3442
BstMCI CGRYCG 1 cut(s) 2064
BstMWI GCNNNNNNNGC 8 cut(s) 163, 314, 709, 815, 1613, 2066, 2165, 2633
BstNI CCWGG 5 cut(s) 125, 1223, 1849, 2932, 3627
BstNSI RCATGY 3 cut(s) 2444, 2640, 3122
BstPAI GACNNNNGTC 1 cut(s) 197
BstSLI GKGCMC 1 cut(s) 2104
BstUI CGCG 4 cut(s) 80, 166, 189, 1212
BstV1I GCAGC 6 cut(s) 1252, 1329, 1434, 3145, 3272, 3409
BstV2I GAAGAC 3 cut(s) 593, 1359, 1464
BstX2I RGATCY 2 cut(s) 2305, 2664
BstXI CCANNNNNNTGG 2 cut(s) 1222, 3648
BstYI RGATCY 2 cut(s) 2305, 2664
Bsu36I CCTNAGG 1 cut(s) 1908
BsuI GTATCC 1 cut(s) 1871
BsuRI GGCC 4 cut(s) 1146, 1256, 1466, 3624
BtgI CCRYGG 2 cut(s) 903, 3670
BtgZI GCGATG 2 cut(s) 884, 2533
BtsCI GGATG 9 cut(s) 480, 1666, 1990, 2080, 2332, 2851, 3461, 3694, 3738
BtsIMutI CAGTG 3 cut(s) 1305, 2119, 2485
BtuMI TCGCGA 1 cut(s) 166
BveI ACCTGC 1 cut(s) 1928
Cac8I GCNNGC 7 cut(s) 84, 965, 1673, 2478, 2509, 2625, 3120
CaiI CAGNNNCTG 1 cut(s) 292
CfoI GCGC 6 cut(s) 82, 94, 642, 1214, 2629, 3442
Cfr10I RCCGGY 1 cut(s) 3725
Cfr13I GGNCC 8 cut(s) 121, 199, 205, 990, 1464, 2172, 2447, 3623
CpoI CGGWCCG 1 cut(s) 199
CseI GACGC 2 cut(s) 1639, 3575
Csp6I GTAC 7 cut(s) 64, 74, 151, 2013, 2762, 2923, 3723
CspAI ACCGGT 1 cut(s) 3725
CspI CGGWCCG 1 cut(s) 199
CviAII CATG 7 cut(s) 904, 2392, 2441, 2453, 2637, 3119, 3653
CviQI GTAC 7 cut(s) 64, 74, 151, 2013, 2762, 2923, 3723
DraI TTTAAA 3 cut(s) 1009, 1881, 2238
DrdI GACNNNNNNGTC 1 cut(s) 3672
DseDI GACNNNNNNGTC 1 cut(s) 3672
Eam1104I CTCTTC 6 cut(s) 389, 428, 555, 723, 756, 3523
EarI CTCTTC 6 cut(s) 389, 428, 555, 723, 756, 3523
EciI GGCGGA 4 cut(s) 47, 239, 698, 1826
Ecl136II GAGCTC 1 cut(s) 1159
Eco130I CCWWGG 6 cut(s) 903, 2104, 3009, 3048, 3125, 3318
Eco147I AGGCCT 1 cut(s) 1146
Eco24I GRGCYC 1 cut(s) 1161
Eco31I GGTCTC 2 cut(s) 3049, 3538
Eco47I GGWCC 6 cut(s) 121, 199, 205, 990, 2172, 2447
Eco53kI GAGCTC 1 cut(s) 1159
Eco57I CTGAAG 2 cut(s) 1329, 1724
Eco81I CCTNAGG 1 cut(s) 1908
Eco88I CYCGRG 2 cut(s) 158, 2912
EcoICRI GAGCTC 1 cut(s) 1159
EcoO109I RGGNCCY 2 cut(s) 121, 2172
EcoRII CCWGG 5 cut(s) 123, 1221, 1847, 2930, 3625
EcoT14I CCWWGG 6 cut(s) 903, 2104, 3009, 3048, 3125, 3318
EcoT38I GRGCYC 1 cut(s) 1161
ErhI CCWWGG 6 cut(s) 903, 2104, 3009, 3048, 3125, 3318
Esp3I CGTCTC 4 cut(s) 29, 185, 451, 2977
FaeI CATG 7 cut(s) 907, 2395, 2444, 2456, 2640, 3122, 3656
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 9 cut(s) 224, 1128, 1981, 2185, 2973, 3111, 3228, 3303, 3375
FatI CATG 7 cut(s) 903, 2391, 2440, 2452, 2636, 3118, 3652
FauI CCCGC 3 cut(s) 1814, 2134, 3166
FauNDI CATATG 1 cut(s) 2557
FbaI TGATCA 1 cut(s) 1188
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 7 cut(s) 1241, 1318, 1423, 3159, 3261, 3423, 3438
FokI GGATG 9 cut(s) 487, 1673, 1997, 2087, 2339, 2858, 3448, 3701, 3725
FriOI GRGCYC 1 cut(s) 1161
Fsp4HI GCNGC 7 cut(s) 1241, 1318, 1423, 3159, 3261, 3423, 3438
FspBI CTAG 9 cut(s) 1557, 1712, 2217, 2477, 2630, 3078, 3126, 3204, 3390
GlaI GCGC 6 cut(s) 81, 93, 641, 1213, 2628, 3441
GluI GCNGC 7 cut(s) 1241, 1318, 1423, 3159, 3261, 3423, 3438
GsaI CCCAGC 2 cut(s) 1059, 2172
GsuI CTGGAG 2 cut(s) 146, 332
HaeII RGCGCY 1 cut(s) 2630
HaeIII GGCC 4 cut(s) 1146, 1256, 1466, 3624
HapII CCGG 7 cut(s) 172, 202, 208, 1462, 1757, 3041, 3726
HgaI GACGC 2 cut(s) 1639, 3575
HhaI GCGC 6 cut(s) 82, 94, 642, 1214, 2629, 3442
Hin1I GRCGYC 1 cut(s) 2986
Hin1II CATG 7 cut(s) 907, 2395, 2444, 2456, 2640, 3122, 3656
Hin6I GCGC 6 cut(s) 80, 92, 640, 1212, 2627, 3440
HinP1I GCGC 6 cut(s) 80, 92, 640, 1212, 2627, 3440
HincII GTYRAC 2 cut(s) 144, 2458
HindII GTYRAC 2 cut(s) 144, 2458
HindIII AAGCTT 1 cut(s) 998
HpaI GTTAAC 1 cut(s) 2458
HpaII CCGG 7 cut(s) 172, 202, 208, 1462, 1757, 3041, 3726
HphI GGTGA 6 cut(s) 1740, 1864, 1924, 1952, 2017, 2747
Hpy166II GTNNAC 5 cut(s) 76, 144, 1305, 2458, 2485
Hpy188III TCNNGA 8 cut(s) 165, 362, 1205, 2309, 2770, 2912, 2960, 3742
Hpy8I GTNNAC 5 cut(s) 76, 144, 1305, 2458, 2485
Hpy99I CGWCG 1 cut(s) 302
HpyCH4III ACNGT 4 cut(s) 2362, 2389, 3508, 3671
HpyCH4IV ACGT 1 cut(s) 2986
HpyF10VI GCNNNNNNNGC 8 cut(s) 163, 314, 709, 815, 1613, 2066, 2165, 2633
HpySE526I ACGT 1 cut(s) 2986
Hsp92I GRCGYC 1 cut(s) 2986
Hsp92II CATG 7 cut(s) 907, 2395, 2444, 2456, 2640, 3122, 3656
HspAI GCGC 6 cut(s) 80, 92, 640, 1212, 2627, 3440
KpnI GGTACC 1 cut(s) 3726
Ksp22I TGATCA 1 cut(s) 1188
KspAI GTTAAC 1 cut(s) 2458
LmnI GCTCC 4 cut(s) 130, 637, 709, 793
Lsp1109I GCAGC 6 cut(s) 1252, 1329, 1434, 3145, 3272, 3409
LweI GCATC 5 cut(s) 2029, 2203, 2322, 2455, 3679
MaeI CTAG 9 cut(s) 1557, 1712, 2217, 2477, 2630, 3078, 3126, 3204, 3390
MaeII ACGT 1 cut(s) 2986
MaeIII GTNAC 3 cut(s) 830, 1637, 1746
MbiI CCGCTC 2 cut(s) 791, 796
MfeI CAATTG 2 cut(s) 1589, 2720
MflI RGATCY 2 cut(s) 2305, 2664
MhlI GDGCHC 4 cut(s) 135, 896, 1161, 2104
MlyI GAGTC 5 cut(s) 58, 139, 149, 170, 932
MmeI TCCRAC 5 cut(s) 549, 638, 696, 1851, 2425
MroXI GAANNNNTTC 1 cut(s) 2715
MslI CAYNNNNRTG 2 cut(s) 372, 3646
MspA1I CMGCKG 2 cut(s) 1733, 3422
MspCI CTTAAG 1 cut(s) 1118
MspI CCGG 7 cut(s) 172, 202, 208, 1462, 1757, 3041, 3726
MunI CAATTG 2 cut(s) 1589, 2720
Mva1269I GAATGC 2 cut(s) 904, 2794
MvaI CCWGG 5 cut(s) 125, 1223, 1849, 2932, 3627
MvnI CGCG 4 cut(s) 80, 166, 189, 1212
MwoI GCNNNNNNNGC 8 cut(s) 163, 314, 709, 815, 1613, 2066, 2165, 2633
NciI CCSGG 5 cut(s) 173, 203, 209, 1463, 1757
NcoI CCATGG 1 cut(s) 903
NdeI CATATG 1 cut(s) 2557
NheI GCTAGC 1 cut(s) 2476
NlaIII CATG 7 cut(s) 907, 2395, 2444, 2456, 2640, 3122, 3656
NlaIV GGNNCC 5 cut(s) 206, 2124, 2173, 2512, 3724
NmuCI GTSAC 2 cut(s) 830, 1746
NruI TCGCGA 1 cut(s) 166
NspI RCATGY 3 cut(s) 2444, 2640, 3122
PaeI GCATGC 1 cut(s) 3122
PaeR7I CTCGAG 2 cut(s) 158, 2912
PaqCI CACCTGC 1 cut(s) 1928
PasI CCCWGGG 1 cut(s) 3626
PceI AGGCCT 1 cut(s) 1146
PciI ACATGT 1 cut(s) 2440
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PctI GAATGC 2 cut(s) 904, 2794
PdmI GAANNNNTTC 1 cut(s) 2715
Pfl23II CGTACG 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 2444
PfoI TCCNGGA 2 cut(s) 171, 207
PinAI ACCGGT 1 cut(s) 3725
PkrI GCNGC 7 cut(s) 1242, 1319, 1424, 3160, 3262, 3424, 3439
Ple19I CGATCG 1 cut(s) 2064
PleI GAGTC 5 cut(s) 57, 139, 148, 169, 932
PpsI GAGTC 5 cut(s) 57, 139, 148, 169, 932
PpuMI RGGWCCY 2 cut(s) 121, 2172
PscI ACATGT 1 cut(s) 2440
PshAI GACNNNNGTC 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 1161
Psp5II RGGWCCY 2 cut(s) 121, 2172
Psp6I CCWGG 5 cut(s) 123, 1221, 1847, 2930, 3625
PspFI CCCAGC 2 cut(s) 1055, 2168
PspGI CCWGG 5 cut(s) 123, 1221, 1847, 2930, 3625
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 5 cut(s) 206, 2124, 2173, 2512, 3724
PspPI GGNCC 8 cut(s) 121, 199, 205, 990, 1464, 2172, 2447, 3623
PspPPI RGGWCCY 2 cut(s) 121, 2172
PspXI VCTCGAGB 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 292
PsuI RGATCY 2 cut(s) 2305, 2664
PvuI CGATCG 1 cut(s) 2064
PvuII CAGCTG 1 cut(s) 1733
RruI TCGCGA 1 cut(s) 166
RsaI GTAC 7 cut(s) 65, 75, 152, 2014, 2763, 2924, 3724
RsaNI GTAC 7 cut(s) 64, 74, 151, 2013, 2762, 2923, 3723
RseI CAYNNNNRTG 2 cut(s) 372, 3646
Rsr2I CGGWCCG 1 cut(s) 199
RsrII CGGWCCG 1 cut(s) 199
SacI GAGCTC 1 cut(s) 1161
SalI GTCGAC 1 cut(s) 142
SatI GCNGC 7 cut(s) 1241, 1318, 1423, 3159, 3261, 3423, 3438
Sau96I GGNCC 8 cut(s) 121, 199, 205, 990, 1464, 2172, 2447, 3623
SchI GAGTC 5 cut(s) 58, 139, 149, 170, 932
SduI GDGCHC 4 cut(s) 135, 896, 1161, 2104
SfaNI GCATC 5 cut(s) 2029, 2203, 2322, 2455, 3679
Sfr274I CTCGAG 2 cut(s) 158, 2912
SinI GGWCC 6 cut(s) 121, 199, 205, 990, 2172, 2447
SlaI CTCGAG 2 cut(s) 158, 2912
SmiMI CAYNNNNRTG 2 cut(s) 372, 3646
SmlI CTYRAG 6 cut(s) 158, 1001, 1118, 1203, 1572, 2912
SmoI CTYRAG 6 cut(s) 158, 1001, 1118, 1203, 1572, 2912
SpeI ACTAGT 1 cut(s) 1556
SphI GCATGC 1 cut(s) 3122
SseBI AGGCCT 1 cut(s) 1146
SspMI CTAG 9 cut(s) 1557, 1712, 2217, 2477, 2630, 3078, 3126, 3204, 3390
SstI GAGCTC 1 cut(s) 1161
StuI AGGCCT 1 cut(s) 1146
StyI CCWWGG 6 cut(s) 903, 2104, 3009, 3048, 3125, 3318
TaaI ACNGT 4 cut(s) 2362, 2389, 3508, 3671
TaiI ACGT 1 cut(s) 2989
TatI WGTACW 1 cut(s) 2012
TauI GCSGC 1 cut(s) 3440
TscAI CASTG 3 cut(s) 1312, 2119, 2492
TseFI GTSAC 2 cut(s) 830, 1746
TseI GCWGC 6 cut(s) 1240, 1317, 1422, 3158, 3260, 3422
Tsp45I GTSAC 2 cut(s) 830, 1746
TspGWI ACGGA 4 cut(s) 263, 1985, 2034, 3556
TspRI CASTG 3 cut(s) 1312, 2119, 2492
Van91I CCANNNNNTGG 1 cut(s) 2444
Vha464I CTTAAG 1 cut(s) 1118
VpaK11BI GGWCC 6 cut(s) 121, 199, 205, 990, 2172, 2447
XapI RAATTY 9 cut(s) 734, 1283, 1388, 1406, 1511, 1801, 2777, 3034, 3112
XceI RCATGY 3 cut(s) 2444, 2640, 3122
XcmI CCANNNNNNNNNTGG 2 cut(s) 1654, 3668
XhoI CTCGAG 2 cut(s) 158, 2912
XmaJI CCTAGG 1 cut(s) 3125
XmiI GTMKAC 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 2715
XspI CTAG 9 cut(s) 1557, 1712, 2217, 2477, 2630, 3078, 3126, 3204, 3390
ZraI GACGTC 1 cut(s) 2987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.