Rorug02G0644600

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000126
Physical Location & Seq
Forward (+)
75522919 .. 75523843
925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0644600.1

Sequence Viewer

Length: 876 bp
ATGTCCCTCTTAATGCTCCAAATACCCTACTTTCCTCTCTTCACTTCTCTCTTGATCCTGGTTATTGTTTGGAAGAAATCCAAAGCCAAATCAGGCCCTAGGAAGTCTCCACCAGGGCCATGGAAGCTGCCTATTATCGGAAACTTGCATCAGTTGGCTACTGGTAATCCACTACCACATCATGCACTGAGAGACTTAGCCAAGAAAAATGGACCTATTATGCACCTAAAACCGGGTCAGGTGGAGGCCTTAATAATCTCTTCCTCCAAAGCTGCAGAAGAGGTGTTGAAGACACATGAGCTCACTTTTGCTCAGAGGCTTCTATTTTTGGCCGCAGAAGTCATGTCTTTTGGTCAAGAAGGCATTGTGTTTGCTCCTTATGGGGATTTCTGGAGAGAGTCATTTAGATCGATAAGAGAAGAAGAGGTTTCGAATCTCATCGAGGCCATTTCCTCATCATCAAAGGGACTTACTCCCATCAATTTCAGTGACAAGAGTTTCAGTTTGACAACTGGGATAGGATCGAGGGCACCGTTTGGGGAAAAGTGCAAAGATCAGGAGGAGTTCTGTTCATTGCTTGAAGAATCGACAAAACTTGCTGGAGGCTTTGACATACCGGATTTGTTTCCTTCACTCAAATGTCTTGGTTTCGTAAATGGGAGCATACCTTCAATGAAGAAGATGCAAAAAAAGTTTGGACAGATTCTTGAGAGTATCATCAACGATCATAAGATCAAAAGTCAAGGCAATGAGATTGACAAACCAGAAGAAGATCTTGTTGACGTACTTCTCAAACTTCAGGAGTCCAAGAAGCTCGAATTCAACGTACTTGACAACCGATCAGATCAAAGATGTCATTATGGTACGTTTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

291

Amino Acids

32.49

Weight (kDa)

8.24

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 37 - 271 6.3e-16 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 529
AciI CCGC 1 cut(s) 333
AclWI GGATC 2 cut(s) 49, 529
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 818
AcuI CTGAAG 1 cut(s) 782
AfaI GTAC 3 cut(s) 786, 828, 865
AfiI CCNNNNNNNGG 2 cut(s) 137, 232
AgsI TTSAA 4 cut(s) 289, 581, 672, 823
AjnI CCWGG 2 cut(s) 57, 112
AluBI AGCT 4 cut(s) 127, 272, 301, 814
AluI AGCT 4 cut(s) 127, 272, 301, 814
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 111, 186
AlwI GGATC 2 cut(s) 49, 529
AoxI GGCC 5 cut(s) 94, 116, 246, 330, 444
ApeKI GCWGC 2 cut(s) 127, 272
ApoI RAATTY 1 cut(s) 818
AspA2I CCTAGG 1 cut(s) 98
AspS9I GGNCC 3 cut(s) 95, 116, 212
AsuC2I CCSGG 1 cut(s) 234
AsuII TTCGAA 1 cut(s) 431
AvaII GGWCC 1 cut(s) 212
AvrII CCTAGG 1 cut(s) 98
BaeGI GKGCMC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 529
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 114, 259
BccI CCATC 1 cut(s) 485
BciT130I CCWGG 2 cut(s) 59, 114
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 111, 186
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 273
BglII AGATCT 1 cut(s) 772
BisI GCNGC 3 cut(s) 128, 273, 333
BlnI CCTAGG 1 cut(s) 98
BlsI GCNGC 3 cut(s) 129, 274, 334
Bme1390I CCNGG 3 cut(s) 59, 114, 234
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 3 cut(s) 95, 116, 212
BmiI GGNNCC 1 cut(s) 531
BmrFI CCNGG 3 cut(s) 59, 114, 234
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 2 cut(s) 157, 672
BmuI ACTGGG 1 cut(s) 522
BpiI GAAGAC 1 cut(s) 296
BpmI CTGGAG 2 cut(s) 412, 621
Bpu14I TTCGAA 1 cut(s) 431
BpuEI CTTGAG 1 cut(s) 728
BpuMI CCSGG 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 98, 113, 119
BsaWI WCCGGW 1 cut(s) 616
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 232
Bse1I ACTGG 2 cut(s) 166, 517
Bse3DI GCAATG 2 cut(s) 572, 754
BseBI CCWGG 2 cut(s) 59, 114
BseCI ATCGAT 1 cut(s) 410
BseDI CCNNGG 3 cut(s) 98, 113, 119
BseLI CCNNNNNNNGG 2 cut(s) 137, 232
BseMI GCAATG 2 cut(s) 572, 754
BseMII CTCAG 2 cut(s) 179, 326
BseNI ACTGG 2 cut(s) 166, 517
BseRI GAGGAG 1 cut(s) 575
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 2 cut(s) 114, 259
BshFI GGCC 5 cut(s) 96, 118, 248, 332, 446
BshNI GGYRCC 1 cut(s) 529
BshVI ATCGAT 1 cut(s) 410
BsiHKAI GWGCWC 1 cut(s) 303
BsiSI CCGG 2 cut(s) 233, 617
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 2 cut(s) 137, 232
BsmAI GTCTC 2 cut(s) 111, 186
BsmFI GGGAC 1 cut(s) 480
BsnI GGCC 5 cut(s) 96, 118, 248, 332, 446
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 2 cut(s) 303, 532
Bsp143I GATC 9 cut(s) 54, 407, 521, 553, 724, 732, 772, 839, 844
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 1 cut(s) 333
BspANI GGCC 5 cut(s) 96, 118, 248, 332, 446
BspCNI CTCAG 2 cut(s) 180, 325
BspDI ATCGAT 1 cut(s) 410
BspLI GGNNCC 1 cut(s) 531
BspMAI CTGCAG 1 cut(s) 277
BspPI GGATC 2 cut(s) 49, 529
BspT104I TTCGAA 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 529
BsrDI GCAATG 2 cut(s) 572, 754
BsrI ACTGG 2 cut(s) 166, 517
BssECI CCNNGG 3 cut(s) 98, 113, 119
BssMI GATC 9 cut(s) 54, 407, 521, 553, 724, 732, 772, 839, 844
BssT1I CCWWGG 2 cut(s) 98, 119
Bst2UI CCWGG 2 cut(s) 59, 114
Bst4CI ACNGT 1 cut(s) 534
Bst6I CTCTTC 4 cut(s) 44, 265, 273, 417
BstBI TTCGAA 1 cut(s) 431
BstDEI CTNAG 3 cut(s) 188, 196, 312
BstDSI CCRYGG 1 cut(s) 119
BstKTI GATC 9 cut(s) 57, 410, 524, 556, 727, 735, 775, 842, 847
BstMAI GTCTC 2 cut(s) 111, 186
BstMBI GATC 9 cut(s) 54, 407, 521, 553, 724, 732, 772, 839, 844
BstMWI GCNNNNNNNGC 1 cut(s) 124
BstNI CCWGG 2 cut(s) 59, 114
BstSCI CCNGG 3 cut(s) 57, 112, 232
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 532
BstV1I GCAGC 2 cut(s) 114, 259
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 772
BstXI CCANNNNNNTGG 1 cut(s) 120
BstYI RGATCY 1 cut(s) 772
Bsu15I ATCGAT 1 cut(s) 410
BsuRI GGCC 5 cut(s) 96, 118, 248, 332, 446
BsuTUI ATCGAT 1 cut(s) 410
BtgI CCRYGG 1 cut(s) 119
BtsIMutI CAGTG 2 cut(s) 185, 493
Cfr13I GGNCC 3 cut(s) 95, 116, 212
ClaI ATCGAT 1 cut(s) 410
Csp6I GTAC 3 cut(s) 785, 827, 864
CviAII CATG 4 cut(s) 120, 182, 296, 343
CviQI GTAC 3 cut(s) 785, 827, 864
DdeI CTNAG 3 cut(s) 188, 196, 312
DpnI GATC 9 cut(s) 56, 409, 523, 555, 726, 734, 774, 841, 846
DpnII GATC 9 cut(s) 54, 407, 521, 553, 724, 732, 772, 839, 844
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 4 cut(s) 44, 265, 273, 417
EarI CTCTTC 4 cut(s) 44, 265, 273, 417
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 98, 119
Eco147I AGGCCT 1 cut(s) 248
Eco24I GRGCYC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 782
EcoICRI GAGCTC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 818
EcoRII CCWGG 2 cut(s) 57, 112
EcoT14I CCWWGG 2 cut(s) 98, 119
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 2 cut(s) 98, 119
FaeI CATG 4 cut(s) 123, 185, 299, 346
FalI AAGNNNNNCTT 2 cut(s) 759, 791
FaqI GGGAC 1 cut(s) 480
FatI CATG 4 cut(s) 119, 181, 295, 342
Fnu4HI GCNGC 3 cut(s) 128, 273, 333
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 3 cut(s) 128, 273, 333
FspBI CTAG 1 cut(s) 99
GluI GCNGC 3 cut(s) 128, 273, 333
GsuI CTGGAG 2 cut(s) 412, 621
HaeIII GGCC 5 cut(s) 96, 118, 248, 332, 446
HapII CCGG 2 cut(s) 233, 617
Hin1II CATG 4 cut(s) 123, 185, 299, 346
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HinfI GANTC 5 cut(s) 398, 433, 584, 703, 803
HpaII CCGG 2 cut(s) 233, 617
Hpy166II GTNNAC 1 cut(s) 781
Hpy188I TCNGA 3 cut(s) 140, 315, 844
Hpy188III TCNNGA 6 cut(s) 52, 356, 391, 557, 707, 800
Hpy8I GTNNAC 1 cut(s) 781
HpyAV CCTTC 3 cut(s) 353, 639, 678
HpyCH4III ACNGT 1 cut(s) 534
HpyCH4IV ACGT 3 cut(s) 783, 825, 866
HpyCH4V TGCA 6 cut(s) 148, 185, 223, 275, 549, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 124
HpyF3I CTNAG 3 cut(s) 188, 196, 312
HpySE526I ACGT 3 cut(s) 783, 825, 866
Hsp92II CATG 4 cut(s) 123, 185, 299, 346
Kzo9I GATC 9 cut(s) 54, 407, 521, 553, 724, 732, 772, 839, 844
LmnI GCTCC 3 cut(s) 21, 379, 660
Lsp1109I GCAGC 2 cut(s) 114, 259
LweI GCATC 2 cut(s) 157, 672
MaeI CTAG 1 cut(s) 99
MaeII ACGT 3 cut(s) 783, 825, 866
MaeIII GTNAC 1 cut(s) 488
MalI GATC 9 cut(s) 56, 409, 523, 555, 726, 734, 774, 841, 846
MboI GATC 9 cut(s) 54, 407, 521, 553, 724, 732, 772, 839, 844
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 2 cut(s) 303, 532
MluCI AATT 2 cut(s) 481, 818
MlyI GAGTC 2 cut(s) 407, 812
MseI TTAA 2 cut(s) 11, 251
MslI CAYNNNNRTG 1 cut(s) 637
MspI CCGG 2 cut(s) 233, 617
MspR9I CCNGG 3 cut(s) 59, 114, 234
MvaI CCWGG 2 cut(s) 59, 114
MwoI GCNNNNNNNGC 1 cut(s) 124
NciI CCSGG 1 cut(s) 234
NcoI CCATGG 1 cut(s) 119
NdeII GATC 9 cut(s) 54, 407, 521, 553, 724, 732, 772, 839, 844
NlaIII CATG 4 cut(s) 123, 185, 299, 346
NlaIV GGNNCC 1 cut(s) 531
NmuCI GTSAC 1 cut(s) 488
NspV TTCGAA 1 cut(s) 431
PceI AGGCCT 1 cut(s) 248
PcsI WCGNNNNNNNCGW 2 cut(s) 530, 822
PfeI GAWTC 3 cut(s) 433, 584, 703
PkrI GCNGC 3 cut(s) 129, 274, 334
PleI GAGTC 2 cut(s) 406, 811
PpsI GAGTC 2 cut(s) 406, 811
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 57, 112
PspGI CCWGG 2 cut(s) 57, 112
PspN4I GGNNCC 1 cut(s) 531
PspPI GGNCC 3 cut(s) 95, 116, 212
PstI CTGCAG 1 cut(s) 277
PsuI RGATCY 1 cut(s) 772
RsaI GTAC 3 cut(s) 786, 828, 865
RsaNI GTAC 3 cut(s) 785, 827, 864
RseI CAYNNNNRTG 1 cut(s) 637
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 11, 251
SatI GCNGC 3 cut(s) 128, 273, 333
Sau3AI GATC 9 cut(s) 54, 407, 521, 553, 724, 732, 772, 839, 844
Sau96I GGNCC 3 cut(s) 95, 116, 212
SchI GAGTC 2 cut(s) 407, 812
ScrFI CCNGG 3 cut(s) 59, 114, 234
SduI GDGCHC 2 cut(s) 303, 532
SfaNI GCATC 2 cut(s) 157, 672
SfcI CTRYAG 1 cut(s) 273
SfuI TTCGAA 1 cut(s) 431
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 637
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
Sse9I AATT 2 cut(s) 481, 818
SseBI AGGCCT 1 cut(s) 248
SsiI CCGC 1 cut(s) 333
SspMI CTAG 1 cut(s) 99
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 3 cut(s) 57, 112, 232
StyI CCWWGG 2 cut(s) 98, 119
TaaI ACNGT 1 cut(s) 534
TaiI ACGT 3 cut(s) 786, 828, 869
TaqI TCGA 6 cut(s) 410, 431, 441, 524, 587, 816
TasI AATT 2 cut(s) 481, 818
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 3 cut(s) 433, 584, 703
Tru1I TTAA 2 cut(s) 11, 251
Tru9I TTAA 2 cut(s) 11, 251
TscAI CASTG 2 cut(s) 192, 493
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 2 cut(s) 127, 272
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 2 cut(s) 561, 689
TspRI CASTG 2 cut(s) 192, 493
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 1 cut(s) 818
XcmI CCANNNNNNNNNTGG 1 cut(s) 117
XmaJI CCTAGG 1 cut(s) 98
XspI CTAG 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.