RLG00000025557

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000126
Physical Location & Seq
Reverse (-)
46664488 .. 46668611
4124 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025557

Sequence Viewer

Length: 2775 bp
ATGGAGAAGATAGCATCCGATTTGAAAGTATCGGAGTTGAAGCAGAGGAGTCTAGGCAAGGCGTACGAGACCTTGCATTCCCAAGCGTCTTCCTTGCTCGTTCTCAGCATTCAATGGAAGGACCTCCAGGAACACTTCGATTCGACCCGGAATGCGATCCGGACTCGTTTCGAAGAGCTTCAAGAACGAGAGAAGCAGTTGGAAGCCAAGGAGTCAAACTTGAAGTCGGAGATGGAATCCAAAAGGGACGAGTTGTTTGGAGTTGAGAAGCTGATTGATGAGAAAGTACAGGAGGTTATAGAAATAAAGAGATCGATTCAAAAGCACAGTGAGGAGATTGAACGTGAAGAGAAGAGAGTCTTGGAAGTACAGAAATTGGTTATAGACAAGGAGAGGGAGTGTAGATTGATCGAAAGACGGATTCAAGAGAGTGGGAAGAAACTGAGTTGGGTTGAGAGAAGAACTGAAGAGAAATTGAATGAGGTTGAGTCTAAGGAGAAGGAACTGAAGAAGTACCGTGAGGATGCTAAGTTGAAAGTGGAGCAACTAAGTGTGATTGATGGGGCAATTGTGGAGCGTAACGAAATCATCGAGTCAAAAGAGGAGAAATTGAGTTGGATTGAGAAGAGTATTGAAGAGAAATCCAAGTTGGCCAACTCTAAGGAGGAGGAAGTGAGAGTGATACAACGATCGTTGAATGTATACCGTGAGAGTATTAAGTCGAAGGAGAGTGACTTAGATGCGATTCTTGAGTCGATTGAAGGGAAGAAGAAAGAATTTGATGTGAAAGAAGAGCAAATTAAAACGTTGCAGAGATTGATTGAGGGATGTGACAAAGAGCTCAAGTTGAAAGAGGAGAAATTAAAACAGGAGAAATTGAAAGAGGAAACACTGAAAGTGAAAGAATACAGTTTGCGAAATAGACAAATGGAGGAGTGGTCCTGCAAGCTTGAATTTAAAGAGAGGGAAGTTGAATTGAAAGAGAAACGATTAGAGTCAAAAATGGAGGAATTTAAAAAATGGGTTGAAGAACGAGGAAAGGATTTGAATTCACTCTCCTCGGAACTGAAAGTTAAAGAGTGTCATCTTGAACAGGCGGCCAAAGAGCTTGAGTTGACTAAGAAACAATGTGGTGGTGTTGCTCAAGTGAAGATTGGGCCATTGGAAAAGACTCCAGAGAACAATGCCATTGTTTCTTCATCTGCAAGTGATCAATCCAACATCAATATTACCGACGGTAGAAGTTTGCAGTTGTTCATGTATGAGCATATGAAGAGAAATGATTCAATAAGCAACAAGATCTCATCCTTTCTCCAACAAAAGTCATCAGAACCAGCAAAATTGGTTTTGGATGCAATGCAAGGGTTTTACACTTCAAATTTGACTGTCGAGAACAAGGAGTTTGATTTGGTGCTTATTAGAAGGACATGCATTCTTTTGTTGGAAGAATTAAAGAGAGTCTCACCCCAAACTAATCCAGAGGTCAGAGAAGAAGCAATGAAGTTGGCCACTGAGTGGAAGGCTAAGATGAAAGTGGGGAGTGAGAACTGGTTGGAGATTTTGGGTTTTTTGCGGCTTGTTACTACTTATGGATTGACCTCTGCTTATAATGAGAAGGAGCTTCACAGTCTTCTTGATATAGTTGCTCAGCATGAACAAGCAACAGAATTATCACGGGCCCTTGCTACCACAGATAAGGCACATGCAAATAGCAACATCTGCTCTCCTATGAAAATTGAAAAAATAAAATCTCCAGTAGCCCAAAATGAAGCAACCATCTCTTCTCCAAATCTTCAGCAAGCTGCCACCACTATTGACGCAAGGAACTTGCAGGGGTTTCAAAAGGAGCAGTTTCGTGAAGCCTGTGATTCTGCTGATTCTGCTTTCAAAAGTATTATTTATGATCTTATTGGAAAGAAGAGACTGATTGAGGCTGTTGGGTTGATTTACACCATGAAGTTATTTGACAAGTTTCCCCCAGTACAAATCTTAAAAGAATATGTTGATAATGGAAAGAGGTGCTGCAGGAATCGAACCAAGAGAAAGAAATCGCTGGATGAAAAGGATATGATTATAGACCAGCATATAGCAGATCTACGAGTTGTTATTCAATGTATTAAAGATTACAACCTCGAGTCTGAATGTCCACCTGAAGAAATTGAATCACAAATTGCTGCGCTTGAAATTGTTAAGGAGCGGAGACGTTTAGCGTCATCTTCTCTTGTCATGAAGGTTGAACAACAGCAAAAACTGCTGAAAATGGTTGAAGTACAAGAACAACAAGAGCAGAAAGCCTCCAAGGGTGAACAACAAGAACAGCAAAAAGAACAGAAAACCTCTGAGGCTAAACTACAAGAAAAAGTACAGCAGAAAGCCTCCAATATGGTTGAAGAACAACAACAGCAACAAGAGAAAAACGCCTCCAAGGTTAAACAGCAAGAACAACAAGAGCAGAAAGCCTCCAAAGTTAAACAACTGCAGAAAAAAGGGAATAAACGTCCAAACAGCACATTTTCCCCCAGGTTTGATCACCAACAGCAGTGTAAAAATAAATTTCAAAGAACATCTATATTTGCTGATAGACACCCGGACCACCCTGACTATCCGAATCCAAGCTCATCAACTTGGACACATGAAAGTAAGGGATGCCCTGAACAGTTTGGTACTGCCGTCAACAAGTATGAAGTTCAGGCTAATTATAAACATCCTGTGCAGTTTGGTATTCCTGCCAATGGTAGCGGTATTTATGCATACCGTGGACAAATCTCGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

925

Amino Acids

107.29

Weight (kDa)

7.48

Isoelectric Point (pI)

60.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 412 - 566 2e-32 Frigida-like protein
Frigida PF07899 629 - 735 4.2e-22 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1608, 2700
AccB7I CCANNNNNTGG 1 cut(s) 1515
AccBSI CCGCTC 1 cut(s) 2197
AccI GTMKAC 1 cut(s) 702
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 4 cut(s) 1097, 1573, 2197, 2739
AclI AACGTT 1 cut(s) 806
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 3 cut(s) 651, 1098, 1506
AcsI RAATTY 6 cut(s) 776, 953, 1010, 1048, 1378, 2552
AcuI CTGAAG 4 cut(s) 486, 527, 1778, 2172
AdeI CACNNNGTG 1 cut(s) 1515
AfaI GTAC 8 cut(s) 65, 288, 369, 515, 1983, 2271, 2364, 2665
AfiI CCNNNNNNNGG 2 cut(s) 1515, 2732
AjnI CCWGG 2 cut(s) 126, 2519
AjuI GAANNNNNNNTTGG 4 cut(s) 344, 376, 632, 664
AluBI AGCT 8 cut(s) 178, 271, 841, 949, 1108, 1621, 1802, 2616
AluI AGCT 8 cut(s) 178, 271, 841, 949, 1108, 1621, 1802, 2616
Alw21I GWGCWC 1 cut(s) 843
Alw26I GTCTC 4 cut(s) 62, 1465, 1915, 2194
AlwI GGATC 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 2132
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 5 cut(s) 651, 1098, 1157, 1506, 1677
ApaI GGGCCC 1 cut(s) 1681
ApeKI GCWGC 3 cut(s) 1802, 2022, 2174
ApoI RAATTY 6 cut(s) 776, 953, 1010, 1048, 1378, 2552
ArsI GACNNNNNNTTYG 4 cut(s) 209, 239, 241, 271
Asp700I GAANNNNTTC 2 cut(s) 177, 1282
AspLEI GCGC 1 cut(s) 2179
AspS9I GGNCC 6 cut(s) 121, 939, 1157, 1677, 1678, 2590
AsuC2I CCSGG 2 cut(s) 148, 2588
AsuHPI GGTGA 3 cut(s) 1455, 2315, 2522
AsuII TTCGAA 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 2132
AvaII GGWCC 3 cut(s) 121, 939, 2590
BaeGI GKGCMC 1 cut(s) 1681
BaeI ACNNNNGTAYC 2 cut(s) 2655, 2688
BalI TGGCCA 2 cut(s) 653, 1508
BanII GRGCYC 2 cut(s) 843, 1681
BbsI GAAGAC 2 cut(s) 81, 1622
Bbv12I GWGCWC 1 cut(s) 843
BbvI GCAGC 3 cut(s) 1789, 2009, 2161
BccI CCATC 3 cut(s) 226, 554, 1784
BceAI ACGGC 1 cut(s) 2654
BciT130I CCWGG 2 cut(s) 128, 2521
BclI TGATCA 2 cut(s) 1210, 2527
BcnI CCSGG 2 cut(s) 148, 2588
BcoDI GTCTC 4 cut(s) 62, 1465, 1915, 2194
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 2 cut(s) 2023, 2477
BglII AGATCT 2 cut(s) 1299, 2092
BisI GCNGC 5 cut(s) 1098, 1574, 1803, 2023, 2175
BlpI GCTNAGC 1 cut(s) 1647
BlsI GCNGC 5 cut(s) 1099, 1575, 1804, 2024, 2176
Bme1390I CCNGG 4 cut(s) 128, 148, 2521, 2588
Bme18I GGWCC 3 cut(s) 121, 939, 2590
BmeT110I CYCGRG 1 cut(s) 2132
BmgT120I GGNCC 6 cut(s) 121, 939, 1157, 1677, 1678, 2590
BmiI GGNNCC 1 cut(s) 1679
BmrFI CCNGG 4 cut(s) 128, 148, 2521, 2588
BmrI ACTGGG 1 cut(s) 1973
BmsI GCATC 5 cut(s) 23, 514, 730, 1342, 2636
BmuI ACTGGG 1 cut(s) 1973
BpiI GAAGAC 2 cut(s) 81, 1622
BpmI CTGGAG 3 cut(s) 110, 1158, 1737
Bpu1102I GCTNAGC 1 cut(s) 1647
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 4 cut(s) 770, 827, 1128, 1130
BpuMI CCSGG 2 cut(s) 148, 2588
Bsa29I ATCGAT 1 cut(s) 314
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 6 cut(s) 207, 1059, 2298, 2424, 2519, 2755
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 4 cut(s) 383, 413, 923, 953
Bsc4I CCNNNNNNNGG 2 cut(s) 1515, 2732
Bse1I ACTGG 3 cut(s) 1553, 1754, 1979
Bse3DI GCAATG 2 cut(s) 1362, 1503
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 2 cut(s) 128, 2521
BseCI ATCGAT 1 cut(s) 314
BseDI CCNNGG 6 cut(s) 207, 1059, 2298, 2424, 2519, 2755
BseGI GGATG 8 cut(s) 14, 529, 833, 1304, 1357, 2062, 2651, 2704
BseLI CCNNNNNNNGG 2 cut(s) 1515, 2732
BseMI GCAATG 2 cut(s) 1362, 1503
BseMII CTCAG 5 cut(s) 118, 434, 1503, 1661, 2331
BseNI ACTGG 3 cut(s) 1553, 1754, 1979
BseRI GAGGAG 7 cut(s) 61, 347, 617, 680, 869, 947, 1048
BseSI GKGCMC 1 cut(s) 1681
BseXI GCAGC 3 cut(s) 1789, 2009, 2161
BsgI GTGCAG 1 cut(s) 2732
Bsh1285I CGRYCG 1 cut(s) 692
BshFI GGCC 5 cut(s) 653, 1100, 1159, 1508, 1679
BshVI ATCGAT 1 cut(s) 314
BsiEI CGRYCG 1 cut(s) 692
BsiHKAI GWGCWC 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 2132
BsiSI CCGG 3 cut(s) 148, 160, 2588
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 2 cut(s) 1515, 2732
BsmAI GTCTC 4 cut(s) 62, 1465, 1915, 2194
BsmBI CGTCTC 1 cut(s) 2194
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 4 cut(s) 76, 108, 157, 1431
BsnI GGCC 5 cut(s) 653, 1100, 1159, 1508, 1679
Bso31I GGTCTC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 2132
Bsp119I TTCGAA 1 cut(s) 171
Bsp120I GGGCCC 1 cut(s) 1677
Bsp1286I GDGCHC 2 cut(s) 843, 1681
Bsp13I TCCGGA 1 cut(s) 159
Bsp143I GATC 9 cut(s) 156, 311, 408, 689, 1210, 1299, 1903, 2092, 2527
Bsp1720I GCTNAGC 1 cut(s) 1647
BspACI CCGC 4 cut(s) 1097, 1573, 2197, 2739
BspANI GGCC 5 cut(s) 653, 1100, 1159, 1508, 1679
BspCNI CTCAG 5 cut(s) 117, 435, 1504, 1660, 2332
BspDI ATCGAT 1 cut(s) 314
BspEI TCCGGA 1 cut(s) 159
BspHI TCATGA 1 cut(s) 2226
BspLI GGNNCC 1 cut(s) 1679
BspMAI CTGCAG 2 cut(s) 2027, 2481
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 2 cut(s) 168, 786
BspT104I TTCGAA 1 cut(s) 171
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 2197
BsrDI GCAATG 2 cut(s) 1362, 1503
BsrI ACTGG 3 cut(s) 1553, 1754, 1979
BssECI CCNNGG 6 cut(s) 207, 1059, 2298, 2424, 2519, 2755
BssMI GATC 9 cut(s) 156, 311, 408, 689, 1210, 1299, 1903, 2092, 2527
BssNAI GTATAC 1 cut(s) 703
BssT1I CCWWGG 3 cut(s) 207, 2298, 2424
Bst1107I GTATAC 1 cut(s) 703
Bst2UI CCWGG 2 cut(s) 128, 2521
Bst4CI ACNGT 9 cut(s) 329, 518, 707, 911, 1238, 1387, 1628, 2658, 2756
BstAPI GCANNNNNTGC 2 cut(s) 1719, 2251
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 947, 1800
BstDSI CCRYGG 1 cut(s) 2755
BstF5I GGATG 8 cut(s) 14, 529, 833, 1304, 1357, 2062, 2651, 2704
BstHHI GCGC 1 cut(s) 2179
BstKTI GATC 9 cut(s) 159, 314, 411, 692, 1213, 1302, 1906, 2095, 2530
BstMAI GTCTC 4 cut(s) 62, 1465, 1915, 2194
BstMBI GATC 9 cut(s) 156, 311, 408, 689, 1210, 1299, 1903, 2092, 2527
BstMCI CGRYCG 1 cut(s) 692
BstMWI GCNNNNNNNGC 3 cut(s) 1719, 1880, 2251
BstNI CCWGG 2 cut(s) 128, 2521
BstNSI RCATGY 2 cut(s) 1431, 1706
BstSCI CCNGG 4 cut(s) 126, 146, 2519, 2586
BstSFI CTRYAG 2 cut(s) 2023, 2477
BstSLI GKGCMC 1 cut(s) 1681
BstV1I GCAGC 3 cut(s) 1789, 2009, 2161
BstV2I GAAGAC 2 cut(s) 81, 1622
BstX2I RGATCY 2 cut(s) 1299, 2092
BstYI RGATCY 2 cut(s) 1299, 2092
BstZ17I GTATAC 1 cut(s) 703
Bsu15I ATCGAT 1 cut(s) 314
BsuRI GGCC 5 cut(s) 653, 1100, 1159, 1508, 1679
BsuTUI ATCGAT 1 cut(s) 314
BtgI CCRYGG 1 cut(s) 2755
BtsCI GGATG 8 cut(s) 14, 529, 833, 1304, 1357, 2062, 2651, 2704
BtsI GCAGTG 1 cut(s) 2546
BtsIMutI CAGTG 4 cut(s) 334, 890, 1509, 2546
Cac8I GCNNGC 2 cut(s) 947, 1800
CciI TCATGA 1 cut(s) 2226
CfoI GCGC 1 cut(s) 2179
Cfr13I GGNCC 6 cut(s) 121, 939, 1157, 1677, 1678, 2590
ClaI ATCGAT 1 cut(s) 314
CseI GACGC 3 cut(s) 75, 1826, 2199
Csp6I GTAC 8 cut(s) 64, 287, 368, 514, 1982, 2270, 2363, 2664
CspCI CAANNNNNGTGG 2 cut(s) 1795, 1830
CviAII CATG 7 cut(s) 1258, 1428, 1652, 1703, 1954, 2227, 2633
CviQI GTAC 8 cut(s) 64, 287, 368, 514, 1982, 2270, 2363, 2664
DpnI GATC 9 cut(s) 158, 313, 410, 691, 1212, 1301, 1905, 2094, 2529
DpnII GATC 9 cut(s) 156, 311, 408, 689, 1210, 1299, 1903, 2092, 2527
DraI TTTAAA 2 cut(s) 958, 1015
DraIII CACNNNGTG 1 cut(s) 1515
EaeI YGGCCR 3 cut(s) 651, 1098, 1506
Ecl136II GAGCTC 1 cut(s) 841
Eco130I CCWWGG 3 cut(s) 207, 2298, 2424
Eco24I GRGCYC 2 cut(s) 843, 1681
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 3 cut(s) 121, 939, 2590
Eco53kI GAGCTC 1 cut(s) 841
Eco57I CTGAAG 4 cut(s) 486, 527, 1778, 2172
Eco88I CYCGRG 1 cut(s) 2132
EcoICRI GAGCTC 1 cut(s) 841
EcoO109I RGGNCCY 2 cut(s) 121, 1678
EcoRI GAATTC 1 cut(s) 1048
EcoRII CCWGG 2 cut(s) 126, 2519
EcoT14I CCWWGG 3 cut(s) 207, 2298, 2424
EcoT22I ATGCAT 2 cut(s) 1433, 2752
EcoT38I GRGCYC 2 cut(s) 843, 1681
ErhI CCWWGG 3 cut(s) 207, 2298, 2424
Esp3I CGTCTC 1 cut(s) 2194
FaeI CATG 7 cut(s) 1261, 1431, 1655, 1706, 1957, 2230, 2636
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FaqI GGGAC 1 cut(s) 260
FatI CATG 7 cut(s) 1257, 1427, 1651, 1702, 1953, 2226, 2632
FauNDI CATATG 1 cut(s) 1269
FbaI TGATCA 2 cut(s) 1210, 2527
FblI GTMKAC 1 cut(s) 702
Fnu4HI GCNGC 5 cut(s) 1098, 1574, 1803, 2023, 2175
FokI GGATG 7 cut(s) 536, 840, 1291, 1364, 2069, 2658, 2691
FriOI GRGCYC 2 cut(s) 843, 1681
Fsp4HI GCNGC 5 cut(s) 1098, 1574, 1803, 2023, 2175
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 2178
GluI GCNGC 5 cut(s) 1098, 1574, 1803, 2023, 2175
GsuI CTGGAG 3 cut(s) 110, 1158, 1737
HaeIII GGCC 5 cut(s) 653, 1100, 1159, 1508, 1679
HapII CCGG 3 cut(s) 148, 160, 2588
HgaI GACGC 3 cut(s) 75, 1826, 2199
HhaI GCGC 1 cut(s) 2179
Hin1II CATG 7 cut(s) 1261, 1431, 1655, 1706, 1957, 2230, 2636
Hin6I GCGC 1 cut(s) 2177
HinP1I GCGC 1 cut(s) 2177
HincII GTYRAC 2 cut(s) 1116, 2674
HindII GTYRAC 2 cut(s) 1116, 2674
HindIII AAGCTT 1 cut(s) 947
HpaII CCGG 3 cut(s) 148, 160, 2588
HphI GGTGA 3 cut(s) 1455, 2315, 2522
Hpy166II GTNNAC 6 cut(s) 703, 1116, 2147, 2306, 2674, 2759
Hpy8I GTNNAC 6 cut(s) 703, 1116, 2147, 2306, 2674, 2759
Hpy99I CGWCG 1 cut(s) 1238
HpyAV CCTTC 8 cut(s) 112, 493, 718, 755, 1416, 1513, 1609, 2224
HpyCH4III ACNGT 9 cut(s) 329, 518, 707, 911, 1238, 1387, 1628, 2658, 2756
HpyCH4IV ACGT 4 cut(s) 343, 806, 2203, 2497
HpyF10VI GCNNNNNNNGC 3 cut(s) 1719, 1880, 2251
HpySE526I ACGT 4 cut(s) 343, 806, 2203, 2497
Hsp92II CATG 7 cut(s) 1261, 1431, 1655, 1706, 1957, 2230, 2636
HspAI GCGC 1 cut(s) 2177
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 2 cut(s) 1210, 2527
Kzo9I GATC 9 cut(s) 156, 311, 408, 689, 1210, 1299, 1903, 2092, 2527
LguI GCTCTTC 2 cut(s) 168, 786
LmnI GCTCC 5 cut(s) 541, 574, 1618, 1846, 2194
Lsp1109I GCAGC 3 cut(s) 1789, 2009, 2161
LweI GCATC 5 cut(s) 23, 514, 730, 1342, 2636
MaeI CTAG 1 cut(s) 53
MaeII ACGT 4 cut(s) 343, 806, 2203, 2497
MaeIII GTNAC 4 cut(s) 578, 731, 830, 1579
MalI GATC 9 cut(s) 158, 313, 410, 691, 1212, 1301, 1905, 2094, 2529
MbiI CCGCTC 1 cut(s) 2197
MboI GATC 9 cut(s) 156, 311, 408, 689, 1210, 1299, 1903, 2092, 2527
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 2 cut(s) 1299, 2092
MhlI GDGCHC 2 cut(s) 843, 1681
MlsI TGGCCA 2 cut(s) 653, 1508
MluNI TGGCCA 2 cut(s) 653, 1508
MmeI TCCRAC 7 cut(s) 180, 207, 596, 1242, 1339, 1422, 1533
Mox20I TGGCCA 2 cut(s) 653, 1508
Mph1103I ATGCAT 2 cut(s) 1433, 2752
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 2 cut(s) 177, 1282
MscI TGGCCA 2 cut(s) 653, 1508
Msp20I TGGCCA 2 cut(s) 653, 1508
MspI CCGG 3 cut(s) 148, 160, 2588
MspR9I CCNGG 4 cut(s) 128, 148, 2521, 2588
MunI CAATTG 1 cut(s) 567
Mva1269I GAATGC 4 cut(s) 76, 108, 157, 1431
MvaI CCWGG 2 cut(s) 128, 2521
MwoI GCNNNNNNNGC 3 cut(s) 1719, 1880, 2251
NciI CCSGG 2 cut(s) 148, 2588
NdeI CATATG 1 cut(s) 1269
NdeII GATC 9 cut(s) 156, 311, 408, 689, 1210, 1299, 1903, 2092, 2527
NlaIII CATG 7 cut(s) 1261, 1431, 1655, 1706, 1957, 2230, 2636
NlaIV GGNNCC 1 cut(s) 1679
NmuCI GTSAC 2 cut(s) 731, 830
NsiI ATGCAT 2 cut(s) 1433, 2752
NspI RCATGY 2 cut(s) 1431, 1706
NspV TTCGAA 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 2132
PagI TCATGA 1 cut(s) 2226
PciSI GCTCTTC 2 cut(s) 168, 786
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PctI GAATGC 4 cut(s) 76, 108, 157, 1431
PdmI GAANNNNTTC 2 cut(s) 177, 1282
Pfl23II CGTACG 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 1515
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 5 cut(s) 1099, 1575, 1804, 2024, 2176
Ple19I CGATCG 1 cut(s) 692
PpuMI RGGWCCY 1 cut(s) 121
PsiI TTATAA 2 cut(s) 1608, 2700
Psp124BI GAGCTC 1 cut(s) 843
Psp1406I AACGTT 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 2 cut(s) 126, 2519
PspGI CCWGG 2 cut(s) 126, 2519
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 1679
PspOMI GGGCCC 1 cut(s) 1677
PspPI GGNCC 6 cut(s) 121, 939, 1157, 1677, 1678, 2590
PspPPI RGGWCCY 1 cut(s) 121
PspXI VCTCGAGB 1 cut(s) 2132
PstI CTGCAG 2 cut(s) 2027, 2481
PsuI RGATCY 2 cut(s) 1299, 2092
PvuI CGATCG 1 cut(s) 692
RsaI GTAC 8 cut(s) 65, 288, 369, 515, 1983, 2271, 2364, 2665
RsaNI GTAC 8 cut(s) 64, 287, 368, 514, 1982, 2270, 2363, 2664
SacI GAGCTC 1 cut(s) 843
SapI GCTCTTC 2 cut(s) 168, 786
SatI GCNGC 5 cut(s) 1098, 1574, 1803, 2023, 2175
Sau3AI GATC 9 cut(s) 156, 311, 408, 689, 1210, 1299, 1903, 2092, 2527
Sau96I GGNCC 6 cut(s) 121, 939, 1157, 1677, 1678, 2590
ScrFI CCNGG 4 cut(s) 128, 148, 2521, 2588
SduI GDGCHC 2 cut(s) 843, 1681
SfaNI GCATC 5 cut(s) 23, 514, 730, 1342, 2636
SfcI CTRYAG 2 cut(s) 2023, 2477
Sfr274I CTCGAG 1 cut(s) 2132
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 3 cut(s) 121, 939, 2590
SlaI CTCGAG 1 cut(s) 2132
SmlI CTYRAG 5 cut(s) 749, 842, 1109, 1143, 2132
SmoI CTYRAG 5 cut(s) 749, 842, 1109, 1143, 2132
SsiI CCGC 4 cut(s) 1097, 1573, 2197, 2739
SspI AATATT 1 cut(s) 1228
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 1 cut(s) 843
StyD4I CCNGG 4 cut(s) 126, 146, 2519, 2586
StyI CCWWGG 3 cut(s) 207, 2298, 2424
TaaI ACNGT 9 cut(s) 329, 518, 707, 911, 1238, 1387, 1628, 2658, 2756
TaiI ACGT 4 cut(s) 346, 809, 2206, 2500
TatI WGTACW 5 cut(s) 286, 367, 1981, 2269, 2362
TauI GCSGC 2 cut(s) 1100, 1576
TscAI CASTG 4 cut(s) 334, 897, 1516, 2546
TseFI GTSAC 2 cut(s) 731, 830
TseI GCWGC 3 cut(s) 1802, 2022, 2174
Tsp45I GTSAC 2 cut(s) 731, 830
TspGWI ACGGA 1 cut(s) 433
TspRI CASTG 4 cut(s) 334, 897, 1516, 2546
Van91I CCANNNNNTGG 1 cut(s) 1515
VpaK11BI GGWCC 3 cut(s) 121, 939, 2590
XapI RAATTY 6 cut(s) 776, 953, 1010, 1048, 1378, 2552
XceI RCATGY 2 cut(s) 1431, 1706
XhoI CTCGAG 1 cut(s) 2132
XmiI GTMKAC 1 cut(s) 702
XmnI GAANNNNTTC 2 cut(s) 177, 1282
XspI CTAG 1 cut(s) 53
Zsp2I ATGCAT 2 cut(s) 1433, 2752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.