Rroxscaffold_6G00430030

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000126
Physical Location & Seq
Forward (+)
50232500 .. 50236591
4092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430030.1

Sequence Viewer

Length: 2310 bp
ATGGAATCCAAAATGAATGAGTTGTTTGGAGTTGAGAAGCTGATTGATGAGAAAGTACAAGAGGTTATAGAAATAAAGAGATCGATACAAAATCATAGTGAGGAGATTGAACGTGAAGAGAAGAGAGTCTTGGAAGTACAGAAATTGGTTATAGACAAGGAGAGGGAGTGTAGTTTGATCGAAAGACGGATTCAAGAGCGTGCAAAGAAACTGAGTTGGGTTGAGAGAAGAACTGAAGAGAAATTGAATGAGGTTGAGTCTAAGGAGAGGGAACTGAAGAAGTACCGTGAGGATGCTAAGTTGAAAGTGGAGCAACTAAGTGTGATTGATGGGGCAATTGTGGACCGCAACGAAATCATTGAGTCAAAAGAAGAGGAAATTAAAGCAGCGCAGATGTCAATCGAAGAATGTGAGAGAAGAATAAAATCAAAAGAGGAGAAATTGAGTTGGATTGAGAAGAGTATTGAAGAGAAATCCAAGTTGGTTAACTCTAAGGAGGAGGAAGTGAGATCGATTGAGTGGAAGAAGAAAGAACTTGATGTGAAAGAAGAGCAAATTAAAACGTTGCACAGATTGCTTGAGGGATGTGAGAAAGAGCTCAAATTGAAAGAGGAGAAATTGAAGGAGGAAACATTGAAAGTGAAAGAGTACAGTGCACGAAATAGACAACTGGAGGGGTGGTCCTACAAGCTTGAATTTAAAGAGAGGGAAGTTGAATTGAAAGAGAAGCAAATAGAGTCTAAAATGGAGGAATTTAAAAAATGGGTTGAACAAAGCGGAAAGGATTTGAATTCACTCTCCTCGGAACTGAAAGTTAAAGAGTGTCAACTTAAACAGGAGGCCAAAGAGCTTGAGTTGATGAAGAAACAATTTGGTGTTGTTGCTCAAGTGAAGATGGGGCCATTGGAAAAGACTCCAGCTGACAATGCCATTGTTTCTTCATCTGCAAGTGATCAATCCAAAATCAATATTACTGATGGTAGAAGTTTGCAGTTGTTCGTGTATGAGCATATGAAGAGAAATGATTTAATAAGCAATAAGATCTCATCCTTTCTCCAACAAAAGTCATCAGAAATAGCAAAATTGGTTTTGGATGCAATGCAAGGGTTTTACTTTTACACTTCAAATTTGACTGTCGAGAACAAGGAGTTTGATTTGGTGCTTATTAGAAGGACATGCATTCTTTTGTTGGAAGAATTAAAGAGAGTCTCACCCCAAACTAATCCAGAGGTCAGAGAAGAAGCAATGAAGTTGGCCAGTGATTGGAAGGCTAAGATGAAAGTGGGGAGTGAGAATTGGTTGGAGATTTTGGGTTTCTTGCGGCTTGTTACTACTTATGGATTGACCTCTGCTTATAACGAGCAGGAGCTTCAAAGTCTTCTTGATATAGTTGCTCAGCATGAACAAGCGACAGAATTATCACGGGCCCTTGCTACCACTGATGAGGCACATGCAAATAGCAACATCTGTACTCCTATGAAAATTAGAAAGACAGAATCTCCGGTAGCCCAAAATGAAGCAACAATCTCTTCTCCAAATCTTCAGCAAGCTGCCACTATTGATGCAAGGAATTTGCAGGGGTTTCAAAATGAGCAGTGTCGTGAAGCCTGTGATTCTGCTTATTCTGCTTTGAGAAGTATTATTTATGATCTTATTGGAAAGAAGAGACTGATTGAGGCTGATAAGATTACAGACCAGCATATAGCAGATCTACGAGTTGTTATTCAATGTATCAAAGATTACAACCTCGAGTCTGAATGTCCACCTGAGGAAATTGAATCACAAATTGCTGTACTTGAAGTTGTTAAGGAGCGGAGACGTTTAGTGTCATCTTCTGTTGTTTTAAGGGTTGAACAACAACAGCAAAAGGTGCTGAAAAATGTTGAAGTACATGAACAAGTAGAGCAGAAAGCCTCCAAGGGTGAACAACAAGAACAGCAAAAAGAACAGAAAACCTCTGAGGCTGAACTACAAGAAAAAGTACAGCAGAAAGCCACCAAAGTTAAACAACAGCAGAAAAAAGGGAATAAACGTCCAAACAGCACGTTTGCCCCCGGGTTTGGTCACCAACAGCAGTGTAAAAATAAATTTCAAAGAACATCTATATTAGCTGATAGAAACCTGGACCACCCTGACTATCCGAATCCAAGTTCATCAACTTGGACACATGGAAGTTATGGATTCCGTGGACAGTTTGGTACTGCCGTCAACAAGCATGAAGTTCATGCTAATCATGGACATCCTGTGCAGTTTGGTATTCCGGCCAATGGTAGAGCTATTTATGCATACAGTGGACAAATCTCGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

769

Amino Acids

88.93

Weight (kDa)

6.22

Isoelectric Point (pI)

62.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 326 - 478 4.7e-28 Frigida-like protein
Frigida PF07899 561 - 607 2.8e-06 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1356
AccB7I CCANNNNNTGG 1 cut(s) 1263
AccBSI CCGCTC 1 cut(s) 1813
AciI CCGC 4 cut(s) 346, 777, 1321, 1813
AclI AACGTT 1 cut(s) 563
AcoI YGGCCR 2 cut(s) 1254, 2262
AcsI RAATTY 6 cut(s) 695, 752, 790, 1126, 1570, 2087
AcuI CTGAAG 3 cut(s) 255, 296, 1526
AfaI GTAC 9 cut(s) 57, 138, 284, 650, 1471, 1794, 1890, 1983, 2200
AfiI CCNNNNNNNGG 3 cut(s) 1263, 2060, 2267
AjnI CCWGG 1 cut(s) 2121
AjuI GAANNNNNNNTTGG 4 cut(s) 113, 145, 464, 496
AluBI AGCT 9 cut(s) 40, 598, 691, 850, 920, 1369, 1550, 2111, 2276
AluI AGCT 9 cut(s) 40, 598, 691, 850, 920, 1369, 1550, 2111, 2276
Alw21I GWGCWC 2 cut(s) 600, 658
Alw26I GTCTC 3 cut(s) 1213, 1660, 1810
Alw44I GTGCAC 1 cut(s) 654
Ama87I CYCGRG 2 cut(s) 1748, 2054
AoxI GGCC 5 cut(s) 840, 899, 1254, 1425, 2262
ApaI GGGCCC 1 cut(s) 1429
ApaLI GTGCAC 1 cut(s) 654
ApeKI GCWGC 2 cut(s) 386, 1550
ApoI RAATTY 6 cut(s) 695, 752, 790, 1126, 1570, 2087
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 6 cut(s) 343, 681, 899, 1425, 1426, 2125
AsuC2I CCSGG 2 cut(s) 2055, 2056
AsuHPI GGTGA 3 cut(s) 1203, 1934, 2057
AvaI CYCGRG 2 cut(s) 1748, 2054
AvaII GGWCC 3 cut(s) 343, 681, 2125
AxyI CCTNAGG 1 cut(s) 1767
BaeGI GKGCMC 2 cut(s) 658, 1429
BaeI ACNNNNGTAYC 2 cut(s) 2190, 2223
BalI TGGCCA 1 cut(s) 1256
BanII GRGCYC 2 cut(s) 600, 1429
BbsI GAAGAC 1 cut(s) 1370
Bbv12I GWGCWC 2 cut(s) 600, 658
BbvI GCAGC 2 cut(s) 398, 1537
BccI CCATC 3 cut(s) 323, 889, 971
BceAI ACGGC 1 cut(s) 2189
BciT130I CCWGG 1 cut(s) 2123
BclI TGATCA 1 cut(s) 952
BcnI CCSGG 2 cut(s) 2055, 2056
BcoDI GTCTC 3 cut(s) 1213, 1660, 1810
BglII AGATCT 2 cut(s) 1041, 1708
BisI GCNGC 3 cut(s) 387, 1322, 1551
BlpI GCTNAGC 1 cut(s) 1395
BlsI GCNGC 3 cut(s) 388, 1323, 1552
Bme1390I CCNGG 3 cut(s) 2055, 2056, 2123
Bme18I GGWCC 3 cut(s) 343, 681, 2125
BmeT110I CYCGRG 2 cut(s) 1748, 2054
BmgT120I GGNCC 6 cut(s) 343, 681, 899, 1425, 1426, 2125
BmiI GGNNCC 2 cut(s) 900, 1427
BmrFI CCNGG 3 cut(s) 2055, 2056, 2123
BmsI GCATC 3 cut(s) 283, 1084, 1552
BpiI GAAGAC 1 cut(s) 1370
BpmI CTGGAG 2 cut(s) 692, 900
Bpu1102I GCTNAGC 1 cut(s) 1395
BpuEI CTTGAG 3 cut(s) 599, 870, 872
BpuMI CCSGG 2 cut(s) 2055, 2056
Bsa29I ATCGAT 2 cut(s) 83, 512
BsaBI GATNNNNATC 1 cut(s) 398
BsaJI CCNNGG 5 cut(s) 801, 1917, 2053, 2054, 2185
BsaWI WCCGGW 1 cut(s) 1501
BsaXI ACNNNNNCTCC 6 cut(s) 152, 182, 665, 695, 1483, 1513
Bsc4I CCNNNNNNNGG 3 cut(s) 1263, 2060, 2267
Bse1I ACTGG 2 cut(s) 675, 1257
Bse21I CCTNAGG 1 cut(s) 1767
Bse3DI GCAATG 2 cut(s) 1104, 1251
Bse8I GATNNNNATC 1 cut(s) 398
BseBI CCWGG 1 cut(s) 2123
BseCI ATCGAT 2 cut(s) 83, 512
BseDI CCNNGG 5 cut(s) 801, 1917, 2053, 2054, 2185
BseGI GGATG 5 cut(s) 298, 590, 1046, 1099, 2239
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 3 cut(s) 1263, 2060, 2267
BseMI GCAATG 2 cut(s) 1104, 1251
BseMII CTCAG 4 cut(s) 203, 1409, 1758, 1950
BseNI ACTGG 2 cut(s) 675, 1257
BseRI GAGGAG 5 cut(s) 116, 449, 512, 626, 790
BseSI GKGCMC 2 cut(s) 658, 1429
BseXI GCAGC 2 cut(s) 398, 1537
BsgI GTGCAG 1 cut(s) 2267
BshFI GGCC 5 cut(s) 842, 901, 1256, 1427, 2264
BshVI ATCGAT 2 cut(s) 83, 512
BsiHKAI GWGCWC 2 cut(s) 600, 658
BsiHKCI CYCGRG 2 cut(s) 1748, 2054
BsiSI CCGG 3 cut(s) 1502, 2055, 2261
BslI CCNNNNNNNGG 3 cut(s) 1263, 2060, 2267
BsmAI GTCTC 3 cut(s) 1213, 1660, 1810
BsmBI CGTCTC 1 cut(s) 1810
BsmI GAATGC 1 cut(s) 1179
BsnI GGCC 5 cut(s) 842, 901, 1256, 1427, 2264
BsoBI CYCGRG 2 cut(s) 1748, 2054
Bsp120I GGGCCC 1 cut(s) 1425
Bsp1286I GDGCHC 3 cut(s) 600, 658, 1429
Bsp143I GATC 7 cut(s) 80, 177, 509, 952, 1041, 1648, 1708
Bsp1720I GCTNAGC 1 cut(s) 1395
BspACI CCGC 4 cut(s) 346, 777, 1321, 1813
BspANI GGCC 5 cut(s) 842, 901, 1256, 1427, 2264
BspCNI CTCAG 4 cut(s) 204, 1408, 1759, 1951
BspDI ATCGAT 2 cut(s) 83, 512
BspLI GGNNCC 2 cut(s) 900, 1427
BspQI GCTCTTC 1 cut(s) 543
BsrBI CCGCTC 1 cut(s) 1813
BsrDI GCAATG 2 cut(s) 1104, 1251
BsrI ACTGG 2 cut(s) 675, 1257
BssECI CCNNGG 5 cut(s) 801, 1917, 2053, 2054, 2185
BssMI GATC 7 cut(s) 80, 177, 509, 952, 1041, 1648, 1708
BssT1I CCWWGG 1 cut(s) 1917
Bst2UI CCWGG 1 cut(s) 2123
Bst4CI ACNGT 5 cut(s) 287, 653, 1135, 2193, 2291
BstAPI GCANNNNNTGC 2 cut(s) 574, 1870
BstC8I GCNNGC 2 cut(s) 201, 1548
BstDEI CTNAG 9 cut(s) 212, 261, 297, 317, 492, 1272, 1395, 1767, 1959
BstDSI CCRYGG 1 cut(s) 2185
BstEII GGTNACC 1 cut(s) 2063
BstF5I GGATG 5 cut(s) 298, 590, 1046, 1099, 2239
BstHHI GCGC 1 cut(s) 391
BstKTI GATC 7 cut(s) 83, 180, 512, 955, 1044, 1651, 1711
BstMAI GTCTC 3 cut(s) 1213, 1660, 1810
BstMBI GATC 7 cut(s) 80, 177, 509, 952, 1041, 1648, 1708
BstMWI GCNNNNNNNGC 5 cut(s) 574, 926, 1625, 1870, 2282
BstNI CCWGG 1 cut(s) 2123
BstNSI RCATGY 2 cut(s) 1179, 1454
BstPI GGTNACC 1 cut(s) 2063
BstSCI CCNGG 3 cut(s) 2053, 2054, 2121
BstSLI GKGCMC 2 cut(s) 658, 1429
BstV1I GCAGC 2 cut(s) 398, 1537
BstV2I GAAGAC 1 cut(s) 1370
BstX2I RGATCY 2 cut(s) 1041, 1708
BstYI RGATCY 2 cut(s) 1041, 1708
Bsu15I ATCGAT 2 cut(s) 83, 512
Bsu36I CCTNAGG 1 cut(s) 1767
BsuRI GGCC 5 cut(s) 842, 901, 1256, 1427, 2264
BsuTUI ATCGAT 2 cut(s) 83, 512
BtgI CCRYGG 1 cut(s) 2185
BtsCI GGATG 5 cut(s) 298, 590, 1046, 1099, 2239
BtsI GCAGTG 2 cut(s) 1601, 2081
BtsIMutI CAGTG 6 cut(s) 658, 1264, 1437, 1601, 2081, 2296
Cac8I GCNNGC 2 cut(s) 201, 1548
CfoI GCGC 1 cut(s) 391
Cfr13I GGNCC 6 cut(s) 343, 681, 899, 1425, 1426, 2125
Cfr9I CCCGGG 1 cut(s) 2054
ClaI ATCGAT 2 cut(s) 83, 512
Csp6I GTAC 9 cut(s) 56, 137, 283, 649, 1470, 1793, 1889, 1982, 2199
CviAII CATG 8 cut(s) 1176, 1400, 1451, 1892, 2168, 2216, 2225, 2234
CviQI GTAC 9 cut(s) 56, 137, 283, 649, 1470, 1793, 1889, 1982, 2199
DdeI CTNAG 9 cut(s) 212, 261, 297, 317, 492, 1272, 1395, 1767, 1959
DpnI GATC 7 cut(s) 82, 179, 511, 954, 1043, 1650, 1710
DpnII GATC 7 cut(s) 80, 177, 509, 952, 1041, 1648, 1708
DraI TTTAAA 2 cut(s) 700, 757
EaeI YGGCCR 2 cut(s) 1254, 2262
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 1 cut(s) 1917
Eco24I GRGCYC 2 cut(s) 600, 1429
Eco47I GGWCC 3 cut(s) 343, 681, 2125
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 3 cut(s) 255, 296, 1526
Eco81I CCTNAGG 1 cut(s) 1767
Eco88I CYCGRG 2 cut(s) 1748, 2054
Eco91I GGTNACC 1 cut(s) 2063
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 1426
EcoO65I GGTNACC 1 cut(s) 2063
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 1 cut(s) 2121
EcoT14I CCWWGG 1 cut(s) 1917
EcoT22I ATGCAT 2 cut(s) 1181, 2287
EcoT38I GRGCYC 2 cut(s) 600, 1429
ErhI CCWWGG 1 cut(s) 1917
Esp3I CGTCTC 1 cut(s) 1810
FaeI CATG 8 cut(s) 1179, 1403, 1454, 1895, 2171, 2219, 2228, 2237
FalI AAGNNNNNCTT 2 cut(s) 113, 145
FatI CATG 8 cut(s) 1175, 1399, 1450, 1891, 2167, 2215, 2224, 2233
FauNDI CATATG 1 cut(s) 1011
FbaI TGATCA 1 cut(s) 952
Fnu4HI GCNGC 3 cut(s) 387, 1322, 1551
FokI GGATG 5 cut(s) 305, 597, 1033, 1106, 2226
FriOI GRGCYC 2 cut(s) 600, 1429
Fsp4HI GCNGC 3 cut(s) 387, 1322, 1551
GlaI GCGC 1 cut(s) 390
GluI GCNGC 3 cut(s) 387, 1322, 1551
GsuI CTGGAG 2 cut(s) 692, 900
HaeIII GGCC 5 cut(s) 842, 901, 1256, 1427, 2264
HapII CCGG 3 cut(s) 1502, 2055, 2261
HhaI GCGC 1 cut(s) 391
Hin1II CATG 8 cut(s) 1179, 1403, 1454, 1895, 2171, 2219, 2228, 2237
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HincII GTYRAC 3 cut(s) 487, 827, 2209
HindII GTYRAC 3 cut(s) 487, 827, 2209
HindIII AAGCTT 1 cut(s) 689
HpaI GTTAAC 1 cut(s) 487
HpaII CCGG 3 cut(s) 1502, 2055, 2261
HphI GGTGA 3 cut(s) 1203, 1934, 2057
Hpy166II GTNNAC 9 cut(s) 343, 487, 656, 827, 1763, 1925, 2189, 2209, 2294
Hpy188I TCNGA 7 cut(s) 805, 1072, 1235, 1756, 1960, 2142, 2305
Hpy188III TCNNGA 5 cut(s) 194, 1138, 1226, 1382, 1601
Hpy8I GTNNAC 9 cut(s) 343, 487, 656, 827, 1763, 1925, 2189, 2209, 2294
HpyAV CCTTC 3 cut(s) 616, 1164, 1261
HpyCH4III ACNGT 5 cut(s) 287, 653, 1135, 2193, 2291
HpyCH4IV ACGT 5 cut(s) 112, 563, 1819, 2032, 2045
HpyF10VI GCNNNNNNNGC 5 cut(s) 574, 926, 1625, 1870, 2282
HpyF3I CTNAG 9 cut(s) 212, 261, 297, 317, 492, 1272, 1395, 1767, 1959
HpySE526I ACGT 5 cut(s) 112, 563, 1819, 2032, 2045
Hsp92II CATG 8 cut(s) 1179, 1403, 1454, 1895, 2171, 2219, 2228, 2237
HspAI GCGC 1 cut(s) 389
Ksp22I TGATCA 1 cut(s) 952
KspAI GTTAAC 1 cut(s) 487
Kzo9I GATC 7 cut(s) 80, 177, 509, 952, 1041, 1648, 1708
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 3 cut(s) 310, 1366, 1810
Lsp1109I GCAGC 2 cut(s) 398, 1537
LweI GCATC 3 cut(s) 283, 1084, 1552
MaeII ACGT 5 cut(s) 112, 563, 1819, 2032, 2045
MaeIII GTNAC 2 cut(s) 1327, 2063
MalI GATC 7 cut(s) 82, 179, 511, 954, 1043, 1650, 1710
MbiI CCGCTC 1 cut(s) 1813
MboI GATC 7 cut(s) 80, 177, 509, 952, 1041, 1648, 1708
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 2 cut(s) 1041, 1708
MhlI GDGCHC 3 cut(s) 600, 658, 1429
MlsI TGGCCA 1 cut(s) 1256
MluNI TGGCCA 1 cut(s) 1256
MlyI GAGTC 7 cut(s) 135, 266, 371, 746, 907, 1215, 1760
MmeI TCCRAC 4 cut(s) 428, 1081, 1170, 1281
Mox20I TGGCCA 1 cut(s) 1256
Mph1103I ATGCAT 2 cut(s) 1181, 2287
MscI TGGCCA 1 cut(s) 1256
Msp20I TGGCCA 1 cut(s) 1256
MspA1I CMGCKG 1 cut(s) 920
MspI CCGG 3 cut(s) 1502, 2055, 2261
MspR9I CCNGG 3 cut(s) 2055, 2056, 2123
MunI CAATTG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 1179
MvaI CCWGG 1 cut(s) 2123
MwoI GCNNNNNNNGC 5 cut(s) 574, 926, 1625, 1870, 2282
NciI CCSGG 2 cut(s) 2055, 2056
NdeI CATATG 1 cut(s) 1011
NdeII GATC 7 cut(s) 80, 177, 509, 952, 1041, 1648, 1708
NlaIII CATG 8 cut(s) 1179, 1403, 1454, 1895, 2171, 2219, 2228, 2237
NlaIV GGNNCC 2 cut(s) 900, 1427
NmuCI GTSAC 1 cut(s) 2063
NsiI ATGCAT 2 cut(s) 1181, 2287
NspI RCATGY 2 cut(s) 1179, 1454
PaeR7I CTCGAG 1 cut(s) 1748
PciSI GCTCTTC 1 cut(s) 543
PctI GAATGC 1 cut(s) 1179
PfeI GAWTC 7 cut(s) 5, 190, 1496, 1613, 1778, 2143, 2181
PflMI CCANNNNNTGG 1 cut(s) 1263
PkrI GCNGC 3 cut(s) 388, 1323, 1552
PleI GAGTC 7 cut(s) 134, 265, 370, 745, 907, 1214, 1759
PpsI GAGTC 7 cut(s) 134, 265, 370, 745, 907, 1214, 1759
PsiI TTATAA 1 cut(s) 1356
Psp124BI GAGCTC 1 cut(s) 600
Psp1406I AACGTT 1 cut(s) 563
Psp6I CCWGG 1 cut(s) 2121
PspEI GGTNACC 1 cut(s) 2063
PspGI CCWGG 1 cut(s) 2121
PspN4I GGNNCC 2 cut(s) 900, 1427
PspOMI GGGCCC 1 cut(s) 1425
PspPI GGNCC 6 cut(s) 343, 681, 899, 1425, 1426, 2125
PspXI VCTCGAGB 1 cut(s) 1748
PsuI RGATCY 2 cut(s) 1041, 1708
PvuII CAGCTG 1 cut(s) 920
RsaI GTAC 9 cut(s) 57, 138, 284, 650, 1471, 1794, 1890, 1983, 2200
RsaNI GTAC 9 cut(s) 56, 137, 283, 649, 1470, 1793, 1889, 1982, 2199
SacI GAGCTC 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 543
SatI GCNGC 3 cut(s) 387, 1322, 1551
Sau3AI GATC 7 cut(s) 80, 177, 509, 952, 1041, 1648, 1708
Sau96I GGNCC 6 cut(s) 343, 681, 899, 1425, 1426, 2125
SchI GAGTC 7 cut(s) 135, 266, 371, 746, 907, 1215, 1760
ScrFI CCNGG 3 cut(s) 2055, 2056, 2123
SduI GDGCHC 3 cut(s) 600, 658, 1429
SfaNI GCATC 3 cut(s) 283, 1084, 1552
Sfr274I CTCGAG 1 cut(s) 1748
SinI GGWCC 3 cut(s) 343, 681, 2125
SlaI CTCGAG 1 cut(s) 1748
SmaI CCCGGG 1 cut(s) 2056
SmlI CTYRAG 4 cut(s) 578, 851, 885, 1748
SmoI CTYRAG 4 cut(s) 578, 851, 885, 1748
SsiI CCGC 4 cut(s) 346, 777, 1321, 1813
SspI AATATT 1 cut(s) 970
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 3 cut(s) 2053, 2054, 2121
StyI CCWWGG 1 cut(s) 1917
TaaI ACNGT 5 cut(s) 287, 653, 1135, 2193, 2291
TaiI ACGT 5 cut(s) 115, 566, 1822, 2035, 2048
TaqI TCGA 6 cut(s) 83, 180, 402, 512, 1137, 1749
TatI WGTACW 7 cut(s) 55, 136, 648, 1469, 1792, 1888, 1981
TauI GCSGC 1 cut(s) 1324
TfiI GAWTC 7 cut(s) 5, 190, 1496, 1613, 1778, 2143, 2181
TscAI CASTG 6 cut(s) 658, 1264, 1444, 1601, 2081, 2296
TseFI GTSAC 1 cut(s) 2063
TseI GCWGC 2 cut(s) 386, 1550
Tsp45I GTSAC 1 cut(s) 2063
TspGWI ACGGA 2 cut(s) 202, 2174
TspMI CCCGGG 1 cut(s) 2054
TspRI CASTG 6 cut(s) 658, 1264, 1444, 1601, 2081, 2296
Van91I CCANNNNNTGG 1 cut(s) 1263
VneI GTGCAC 1 cut(s) 654
VpaK11BI GGWCC 3 cut(s) 343, 681, 2125
XapI RAATTY 6 cut(s) 695, 752, 790, 1126, 1570, 2087
XceI RCATGY 2 cut(s) 1179, 1454
XhoI CTCGAG 1 cut(s) 1748
XmaI CCCGGG 1 cut(s) 2054
Zsp2I ATGCAT 2 cut(s) 1181, 2287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.