MD04G1214600.v1.1

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000126
Physical Location & Seq
Reverse (-)
29777697 .. 29781754
4058 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1214600.v1.1.491

Sequence Viewer

Length: 2619 bp
ATGGAGAAGATTCAGTCGGATTTGAGAATCTCGGAGGAGAGGCAGAGCAGCCTCTGCAGAGCGTACGAGAGCTTGCATGCGGAAGCGAACTCCGTCGTTCTGTTTTCTGTGCAATGGAAGGAGCTCGAGGAGCACTTCGAGTCGACCCGTAGCGAGATCCGGACCCGATTACAAGAACTCGAGAACCGCGAGAAGGAGATGGACGATCTTGGGAAGCGGCTGGAGGCGTCGGAGTTGAGGTTTGACTCGGAGATGGCGGCCAAAGCTTTGGAATTGCGCGGAATCGAGAAGTCGATTGCGGAGAGAGTGAAGGAGTATGAATCTGGTAAGCTCCGCTTGAAATCGGTTGATATGTCGATTGATGTTAAAAAGAGGCAAGTTTCGGAAGTTGAGAGCTTGGTTAGAGAGAAGGAGAGGGAGCGGGAATCGATGGAGAGATGTATCGAAGAGCGGAAAAAGATATTGAATTGGTTTGTGAAAAATGCGGAAGAGAGGTCTGTAGAGATTGAGGCTAAAGAGAAGGAAGTGGAAGAGGTCAGGACGGTGTTGAGCAAGTACGGAGATGATATTGAGTTGAAAGAGAGGCGGTTAAATTCGATTGTGGAGTCGATTGAGGAGCGGACGAAAGTGTATGATTTGAGAGAGGAGGAGATTAGAAGGGCGGAAAGATCGATTGAAGAATGTGACAAGAAGATGAAATTGAGAAAGGAGAAACTCGGTTTGATTGAGAAATCGATCAAAGGATGTGAGGAAGAGATGAAATCGAAAGAGGAGAAACTTAGTTTGATTCGGAAGTCATTGGCCGAATGCTCTAATACACTGGAATCCAGAGAGAATGCAATTAGGGAATTGGAGTTGAAAGAGAGGGATTTTTGTTTGCTCAAGAAACTGATGGGGGAGTGGTCCTGTAAACTTGAATATAAAGAAAGGGAACTCGGCGGATGGTTAGAGAAGCTGGAACTGAAAGAAAAAGGATTTGAGCAGAAACTAGAGGAACTTCATTTGATTGATAAGAGGGTTAATGAATTCCTCGATGAGGTTCGGTTGAAAGAAAAGCATTTGGATTCACTCCAACAAACGATACATGAACATGAAAAGCGTTTGGATTCACTCTCCAATGGACTTCAAGTCAAAGAAAGGAAACTTGAACTACAGGCCAGAGCGCTTGAATTTAAGCAGAAAAAGTTTGATTCTATTCGTAAATCCGCTGAAGAACGTTCCAAGAAAATGTCATTGGAGAAGACTAATATCCTTAATTCTCAAGTGAAGACTGAGCAATTGGAACATAATCCTGCTAACAATCCCTCTGTTCCTTTGCCCGCAAGTATCCAATCCCGCATCAACATGAGTGGTAAGGACTTGCAGTTGTTTCTGAACGAGCATTTGAAGAGACATGATTTATTGGGTACCGAAATCTCAGGGATTCTTCAAGCGTCATCAGACCCAGCCAAATTAGTTTTGGATGCAATGCAAGGGTTTTACCCTTCAAGTTTGGTTGTGGAAAATAAGGAGTGTGATTTTGACGTGAGCGTTATTAGGAGGAGTTGTATTCTTTTGTTACAGGAGTTAAAGAGAGTCTCACCGCAAATTAATCCTCCGGTGAGAGAAGAAGCAAGAAAGTTGGCAGGTGACTGGAAGGACAAGATGATGGTGGCAGTAGAAAATGGGTTGGAGGTTCTGGGTTTTTTGCTGCTTATTGCTACATTTGACTTGACCTCCGCTTATCCTGAGAGTGAGCTTCAAAGTCTTCTTCTTGTGGCTGGTCAGCAAGCACACGTTGCACAGGCTGCTGGATTGAGCCTTGGCATCTCAGATAAGGCACCTGTGTGCAGCATAACCTCTGTCAAAATTGTGGAACCGGAATCTTCACTGGCCAAAAGTGAAGCAACTTTATCTTCTCCAAATTTTCAACCAAGTGCCACCATTGATGCAAGGACTTTGCAGGGGATTTTAATTGAGCATATGAAGCAAAATCATTCAATGCAAAAAGACATGTTGGCAGCTCTTCAATTGTCATCGGACCCAGCAAAACTTGTGTTGGATGTGATTCAAACATCTTTTGCTCGATACTGGAGTAGAGGAGAGGTTGCTTTAGATGAAACTTTCATGTCAAGCACGATCAACCTTTTGGAGGAGCTAATGAGAGTCTCTCCGAATGTTGGACCTCATGAGAAAGAGGATGCAATAAAGCTAGCATACACGTGGGAAGCAAAAATGAAAGGCAACACTGAAAATTCATTGGAGAGTTTGGGATATTTGCAGTTTTTAGCTACATACGGATTGCTTTATCGTTTAAAGGAAGATGAGATCAAAAAGATTCTTGGGATGATTTGTCAGCATAAGCAGGCCCTAAAAATATGCCAGACACTTGGTTTTGTCCCAGAATTTGTTAAGAAGCTAATTGAGAGGAAGCAACTTTTAGAGGCTGCTAGACTTATTTGCACCTTCAAGATAGCTGAAAAGTTACCCCCGGTACCGCTTCTAAAAGAATATGTGGAGGGTGCAAGGAAGTCTTGCAGGACAATGTGGAGGAAAAAGAAATCACTTGATGAAAAGGTACTTTCACTCTTTTTTGGACCATTTGATATCTGCTTCCTGAATCTAAGCCATCCAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

873

Amino Acids

100.48

Weight (kDa)

6.12

Isoelectric Point (pI)

55.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 448 - 595 7.7e-30 Frigida-like protein
Frigida PF07899 640 - 848 4.9e-42 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1616
Acc36I ACCTGC 1 cut(s) 1616
Acc65I GGTACC 2 cut(s) 1406, 2473
AccB1I GGYRCC 3 cut(s) 1406, 1819, 2473
AccBSI CCGCTC 3 cut(s) 421, 451, 619
AccI GTMKAC 1 cut(s) 143
AccII CGCG 2 cut(s) 189, 279
AccIII TCCGGA 1 cut(s) 159
AclI AACGTT 1 cut(s) 1216
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 3 cut(s) 258, 801, 1872
AcsI RAATTY 6 cut(s) 592, 1025, 1169, 1903, 2233, 2384
AcuI CTGAAG 1 cut(s) 1230
AcvI CACGTG 1 cut(s) 2202
AcyI GRCGYC 1 cut(s) 227
AfaI GTAC 5 cut(s) 65, 557, 1408, 2475, 2559
AfeI AGCGCT 1 cut(s) 1164
AfiI CCNNNNNNNGG 4 cut(s) 193, 1036, 2131, 2159
AflIII ACRYGT 3 cut(s) 1774, 1992, 2199
AhdI GACNNNNNGTC 1 cut(s) 1127
AjiI CACGTC 1 cut(s) 1525
AjuI GAANNNNNNNTTGG 2 cut(s) 1217, 1249
AleI CACNNNNGTG 1 cut(s) 1825
Alw21I GWGCWC 2 cut(s) 126, 135
Alw26I GTCTC 3 cut(s) 1384, 1582, 2152
AlwI GGATC 1 cut(s) 151
AlwNI CAGNNNCTG 1 cut(s) 54
Ama87I CYCGRG 2 cut(s) 125, 179
Aor13HI TCCGGA 1 cut(s) 159
Aor51HI AGCGCT 1 cut(s) 1164
AoxI GGCC 5 cut(s) 258, 801, 1155, 1872, 2346
ApeKI GCWGC 6 cut(s) 48, 1690, 1787, 1830, 2000, 2426
ApoI RAATTY 6 cut(s) 592, 1025, 1169, 1903, 2233, 2384
AseI ATTAAT 1 cut(s) 1590
Asp718I GGTACC 2 cut(s) 1406, 2473
AspLEI GCGC 2 cut(s) 279, 1165
AspS9I GGNCC 6 cut(s) 162, 903, 2020, 2162, 2347, 2576
AsuC2I CCSGG 1 cut(s) 2471
AsuHPI GGTGA 3 cut(s) 1572, 1612, 1640
AsuNHI GCTAGC 1 cut(s) 2191
AvaI CYCGRG 2 cut(s) 125, 179
AvaII GGWCC 5 cut(s) 162, 903, 2020, 2162, 2576
BaeI ACNNNNGTAYC 2 cut(s) 2059, 2092
BalI TGGCCA 1 cut(s) 1874
BanI GGYRCC 3 cut(s) 1406, 1819, 2473
BanII GRGCYC 1 cut(s) 126
BbrPI CACGTG 1 cut(s) 2202
BbsI GAAGAC 3 cut(s) 1247, 1274, 1739
Bbv12I GWGCWC 2 cut(s) 126, 135
BbvI GCAGC 6 cut(s) 60, 1677, 1774, 1842, 2012, 2413
BccI CCATC 7 cut(s) 193, 247, 424, 886, 936, 1642, 2616
BciVI GTATCC 1 cut(s) 1337
BcnI CCSGG 1 cut(s) 2471
BcoDI GTCTC 3 cut(s) 1384, 1582, 2152
BfaI CTAG 3 cut(s) 989, 2192, 2430
BfmI CTRYAG 3 cut(s) 55, 498, 1151
BfoI RGCGCY 1 cut(s) 1166
BfuAI ACCTGC 1 cut(s) 1616
BfuI GTATCC 1 cut(s) 1337
BisI GCNGC 8 cut(s) 49, 218, 258, 1691, 1788, 1831, 2001, 2427
BlsI GCNGC 8 cut(s) 50, 219, 259, 1692, 1789, 1832, 2002, 2428
Bme1390I CCNGG 1 cut(s) 2471
Bme18I GGWCC 5 cut(s) 162, 903, 2020, 2162, 2576
BmeRI GACNNNNNGTC 1 cut(s) 1127
BmeT110I CYCGRG 2 cut(s) 125, 179
BmgBI CACGTC 1 cut(s) 1525
BmgT120I GGNCC 6 cut(s) 162, 903, 2020, 2162, 2347, 2576
BmiI GGNNCC 6 cut(s) 164, 1408, 1821, 1857, 2022, 2475
BmrFI CCNGG 1 cut(s) 2471
BmsI GCATC 5 cut(s) 1347, 1453, 1815, 1918, 2170
BmtI GCTAGC 1 cut(s) 2195
BpiI GAAGAC 3 cut(s) 1247, 1274, 1739
BplI GAGNNNNNCTC 2 cut(s) 2134, 2166
BpmI CTGGAG 2 cut(s) 242, 2092
BpuEI CTTGAG 2 cut(s) 866, 1245
BpuMI CCSGG 1 cut(s) 2471
Bsa29I ATCGAT 3 cut(s) 428, 671, 734
BsaAI YACGTR 1 cut(s) 2202
BsaHI GRCGYC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 1801, 2469
BsaWI WCCGGW 3 cut(s) 159, 1597, 1858
BsaXI ACNNNNNCTCC 6 cut(s) 224, 254, 1531, 1561, 1700, 1730
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 1036, 2131, 2159
Bse1I ACTGG 4 cut(s) 825, 1637, 1875, 2075
Bse3DI GCAATG 2 cut(s) 119, 1473
BseAI TCCGGA 1 cut(s) 159
BseCI ATCGAT 3 cut(s) 428, 671, 734
BseDI CCNNGG 2 cut(s) 1801, 2469
BseGI GGATG 7 cut(s) 749, 947, 1468, 2047, 2185, 2331, 2608
BseLI CCNNNNNNNGG 4 cut(s) 193, 1036, 2131, 2159
BseMI GCAATG 2 cut(s) 119, 1473
BseMII CTCAG 4 cut(s) 1263, 1431, 1719, 1824
BseNI ACTGG 4 cut(s) 825, 1637, 1875, 2075
BseRI GAGGAG 9 cut(s) 50, 143, 629, 659, 662, 785, 1555, 2094, 2147
BseXI GCAGC 6 cut(s) 60, 1677, 1774, 1842, 2012, 2413
BseYI CCCAGC 2 cut(s) 1444, 2023
BsgI GTGCAG 1 cut(s) 1849
Bsh1236I CGCG 2 cut(s) 189, 279
BshFI GGCC 5 cut(s) 260, 803, 1157, 1874, 2348
BshNI GGYRCC 3 cut(s) 1406, 1819, 2473
BshVI ATCGAT 3 cut(s) 428, 671, 734
BsiHKAI GWGCWC 2 cut(s) 126, 135
BsiHKCI CYCGRG 2 cut(s) 125, 179
BsiSI CCGG 4 cut(s) 160, 1598, 1859, 2471
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 2363
BslI CCNNNNNNNGG 4 cut(s) 193, 1036, 2131, 2159
BsmAI GTCTC 3 cut(s) 1384, 1582, 2152
BsmFI GGGAC 1 cut(s) 2363
BsmI GAATGC 2 cut(s) 812, 841
BsnI GGCC 5 cut(s) 260, 803, 1157, 1874, 2348
BsoBI CYCGRG 2 cut(s) 125, 179
Bsp1286I GDGCHC 2 cut(s) 126, 135
Bsp13I TCCGGA 1 cut(s) 159
Bsp143I GATC 6 cut(s) 156, 205, 668, 735, 2118, 2307
BspANI GGCC 5 cut(s) 260, 803, 1157, 1874, 2348
BspCNI CTCAG 4 cut(s) 1264, 1430, 1720, 1823
BspDI ATCGAT 3 cut(s) 428, 671, 734
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 2 cut(s) 189, 279
BspHI TCATGA 1 cut(s) 2167
BspLI GGNNCC 6 cut(s) 164, 1408, 1821, 1857, 2022, 2475
BspMAI CTGCAG 1 cut(s) 59
BspMI ACCTGC 1 cut(s) 1616
BspOI GCTAGC 1 cut(s) 2195
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 2 cut(s) 441, 2010
BspT107I GGYRCC 3 cut(s) 1406, 1819, 2473
BsrBI CCGCTC 3 cut(s) 421, 451, 619
BsrDI GCAATG 2 cut(s) 119, 1473
BsrI ACTGG 4 cut(s) 825, 1637, 1875, 2075
BssECI CCNNGG 2 cut(s) 1801, 2469
BssMI GATC 6 cut(s) 156, 205, 668, 735, 2118, 2307
BssNI GRCGYC 1 cut(s) 227
BssT1I CCWWGG 1 cut(s) 1801
Bst4CI ACNGT 1 cut(s) 544
Bst6I CTCTTC 6 cut(s) 441, 483, 525, 747, 1382, 2010
BstACI GRCGYC 1 cut(s) 227
BstAPI GCANNNNNTGC 3 cut(s) 54, 1778, 1787
BstBAI YACGTR 1 cut(s) 2202
BstC8I GCNNGC 6 cut(s) 74, 78, 1320, 1770, 2193, 2346
BstDEI CTNAG 6 cut(s) 779, 1272, 1417, 1728, 1810, 2603
BstENI CCTNNNNNAGG 2 cut(s) 1034, 2129
BstF5I GGATG 7 cut(s) 749, 947, 1468, 2047, 2185, 2331, 2608
BstFNI CGCG 2 cut(s) 189, 279
BstH2I RGCGCY 1 cut(s) 1166
BstHHI GCGC 2 cut(s) 279, 1165
BstKTI GATC 6 cut(s) 159, 208, 671, 738, 2121, 2310
BstMAI GTCTC 3 cut(s) 1384, 1582, 2152
BstMBI GATC 6 cut(s) 156, 205, 668, 735, 2118, 2307
BstMWI GCNNNNNNNGC 6 cut(s) 54, 130, 263, 1778, 1787, 1966
BstNSI RCATGY 2 cut(s) 80, 1996
BstSCI CCNGG 1 cut(s) 2469
BstSFI CTRYAG 3 cut(s) 55, 498, 1151
BstUI CGCG 2 cut(s) 189, 279
BstV1I GCAGC 6 cut(s) 60, 1677, 1774, 1842, 2012, 2413
BstV2I GAAGAC 3 cut(s) 1247, 1274, 1739
BstX2I RGATCY 1 cut(s) 156
BstXI CCANNNNNNTGG 2 cut(s) 268, 2369
BstYI RGATCY 1 cut(s) 156
Bsu15I ATCGAT 3 cut(s) 428, 671, 734
BsuI GTATCC 1 cut(s) 1337
BsuRI GGCC 5 cut(s) 260, 803, 1157, 1874, 2348
BsuTUI ATCGAT 3 cut(s) 428, 671, 734
BtrI CACGTC 1 cut(s) 1525
BtsCI GGATG 7 cut(s) 749, 947, 1468, 2047, 2185, 2331, 2608
BtsIMutI CAGTG 3 cut(s) 818, 1868, 2226
BveI ACCTGC 1 cut(s) 1616
Cac8I GCNNGC 6 cut(s) 74, 78, 1320, 1770, 2193, 2346
CaiI CAGNNNCTG 1 cut(s) 54
CciI TCATGA 1 cut(s) 2167
CfoI GCGC 2 cut(s) 279, 1165
Cfr13I GGNCC 6 cut(s) 162, 903, 2020, 2162, 2347, 2576
ClaI ATCGAT 3 cut(s) 428, 671, 734
CseI GACGC 2 cut(s) 216, 1422
Csp6I GTAC 5 cut(s) 64, 556, 1407, 2474, 2558
CspCI CAANNNNNGTGG 2 cut(s) 1330, 1365
CviAII CATG 8 cut(s) 77, 1085, 1091, 1345, 1394, 1993, 2107, 2168
CviQI GTAC 5 cut(s) 64, 556, 1407, 2474, 2558
DdeI CTNAG 6 cut(s) 779, 1272, 1417, 1728, 1810, 2603
DpnI GATC 6 cut(s) 158, 207, 670, 737, 2120, 2309
DpnII GATC 6 cut(s) 156, 205, 668, 735, 2118, 2307
DraI TTTAAA 1 cut(s) 2295
DriI GACNNNNNGTC 1 cut(s) 1127
EaeI YGGCCR 3 cut(s) 258, 801, 1872
Eam1104I CTCTTC 6 cut(s) 441, 483, 525, 747, 1382, 2010
Eam1105I GACNNNNNGTC 1 cut(s) 1127
EarI CTCTTC 6 cut(s) 441, 483, 525, 747, 1382, 2010
EciI GGCGGA 2 cut(s) 677, 954
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 1801
Eco24I GRGCYC 1 cut(s) 126
Eco32I GATATC 1 cut(s) 2587
Eco47I GGWCC 5 cut(s) 162, 903, 2020, 2162, 2576
Eco47III AGCGCT 1 cut(s) 1164
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 1230
Eco72I CACGTG 1 cut(s) 2202
Eco88I CYCGRG 2 cut(s) 125, 179
EcoICRI GAGCTC 1 cut(s) 124
EcoNI CCTNNNNNAGG 2 cut(s) 1034, 2129
EcoO109I RGGNCCY 1 cut(s) 2347
EcoRI GAATTC 1 cut(s) 1025
EcoRV GATATC 1 cut(s) 2587
EcoT14I CCWWGG 1 cut(s) 1801
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 1801
FaeI CATG 8 cut(s) 80, 1088, 1094, 1348, 1397, 1996, 2110, 2171
FalI AAGNNNNNCTT 4 cut(s) 320, 352, 2497, 2529
FaqI GGGAC 1 cut(s) 2363
FatI CATG 8 cut(s) 76, 1084, 1090, 1344, 1393, 1992, 2106, 2167
FauI CCCGC 3 cut(s) 414, 1327, 1343
FauNDI CATATG 1 cut(s) 1962
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 8 cut(s) 49, 218, 258, 1691, 1788, 1831, 2001, 2427
FokI GGATG 7 cut(s) 756, 954, 1475, 2054, 2192, 2338, 2595
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 8 cut(s) 49, 218, 258, 1691, 1788, 1831, 2001, 2427
FspBI CTAG 3 cut(s) 989, 2192, 2430
GlaI GCGC 2 cut(s) 278, 1164
GluI GCNGC 8 cut(s) 49, 218, 258, 1691, 1788, 1831, 2001, 2427
GsaI CCCAGC 2 cut(s) 1448, 2027
GsuI CTGGAG 2 cut(s) 242, 2092
HaeII RGCGCY 1 cut(s) 1166
HaeIII GGCC 5 cut(s) 260, 803, 1157, 1874, 2348
HapII CCGG 4 cut(s) 160, 1598, 1859, 2471
HgaI GACGC 2 cut(s) 216, 1422
HhaI GCGC 2 cut(s) 279, 1165
Hin1I GRCGYC 1 cut(s) 227
Hin1II CATG 8 cut(s) 80, 1088, 1094, 1348, 1397, 1996, 2110, 2171
Hin6I GCGC 2 cut(s) 277, 1163
HinP1I GCGC 2 cut(s) 277, 1163
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HindIII AAGCTT 1 cut(s) 264
HpaII CCGG 4 cut(s) 160, 1598, 1859, 2471
HphI GGTGA 3 cut(s) 1572, 1612, 1640
Hpy166II GTNNAC 2 cut(s) 144, 911
Hpy8I GTNNAC 2 cut(s) 144, 911
Hpy99I CGWCG 2 cut(s) 98, 232
HpyAV CCTTC 9 cut(s) 112, 187, 304, 403, 514, 651, 1494, 1630, 2455
HpyCH4III ACNGT 1 cut(s) 544
HpyCH4IV ACGT 4 cut(s) 1216, 1524, 1776, 2201
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 130, 263, 1778, 1787, 1966
HpyF3I CTNAG 6 cut(s) 779, 1272, 1417, 1728, 1810, 2603
HpySE526I ACGT 4 cut(s) 1216, 1524, 1776, 2201
Hsp92I GRCGYC 1 cut(s) 227
Hsp92II CATG 8 cut(s) 80, 1088, 1094, 1348, 1397, 1996, 2110, 2171
HspAI GCGC 2 cut(s) 277, 1163
Kpn2I TCCGGA 1 cut(s) 159
KpnI GGTACC 2 cut(s) 1410, 2477
Kzo9I GATC 6 cut(s) 156, 205, 668, 735, 2118, 2307
LguI GCTCTTC 2 cut(s) 441, 2010
LmnI GCTCC 6 cut(s) 121, 130, 336, 418, 616, 2134
Lsp1109I GCAGC 6 cut(s) 60, 1677, 1774, 1842, 2012, 2413
LweI GCATC 5 cut(s) 1347, 1453, 1815, 1918, 2170
MaeI CTAG 3 cut(s) 989, 2192, 2430
MaeII ACGT 4 cut(s) 1216, 1524, 1776, 2201
MaeIII GTNAC 4 cut(s) 683, 1557, 1628, 2463
MalI GATC 6 cut(s) 158, 207, 670, 737, 2120, 2309
MbiI CCGCTC 3 cut(s) 421, 451, 619
MboI GATC 6 cut(s) 156, 205, 668, 735, 2118, 2307
MfeI CAATTG 2 cut(s) 1277, 2009
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 2 cut(s) 126, 135
MlsI TGGCCA 1 cut(s) 1874
MluNI TGGCCA 1 cut(s) 1874
MlyI GAGTC 5 cut(s) 149, 239, 614, 1584, 2154
MmeI TCCRAC 5 cut(s) 210, 1096, 1650, 2019, 2140
Mox20I TGGCCA 1 cut(s) 1874
MroI TCCGGA 1 cut(s) 159
MscI TGGCCA 1 cut(s) 1874
MslI CAYNNNNRTG 4 cut(s) 1089, 1343, 1825, 2200
Msp20I TGGCCA 1 cut(s) 1874
MspA1I CMGCKG 1 cut(s) 1208
MspI CCGG 4 cut(s) 160, 1598, 1859, 2471
MspR9I CCNGG 1 cut(s) 2471
MunI CAATTG 2 cut(s) 1277, 2009
Mva1269I GAATGC 2 cut(s) 812, 841
MvnI CGCG 2 cut(s) 189, 279
MwoI GCNNNNNNNGC 6 cut(s) 54, 130, 263, 1778, 1787, 1966
NciI CCSGG 1 cut(s) 2471
NdeI CATATG 1 cut(s) 1962
NdeII GATC 6 cut(s) 156, 205, 668, 735, 2118, 2307
NheI GCTAGC 1 cut(s) 2191
NlaIII CATG 8 cut(s) 80, 1088, 1094, 1348, 1397, 1996, 2110, 2171
NlaIV GGNNCC 6 cut(s) 164, 1408, 1821, 1857, 2022, 2475
NmeAIII GCCGAG 1 cut(s) 915
NmuCI GTSAC 2 cut(s) 683, 1628
NspI RCATGY 2 cut(s) 80, 1996
OliI CACNNNNGTG 1 cut(s) 1825
PaeI GCATGC 1 cut(s) 80
PaeR7I CTCGAG 2 cut(s) 125, 179
PagI TCATGA 1 cut(s) 2167
PaqCI CACCTGC 1 cut(s) 1616
PciI ACATGT 1 cut(s) 1992
PciSI GCTCTTC 2 cut(s) 441, 2010
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PctI GAATGC 2 cut(s) 812, 841
Pfl23II CGTACG 1 cut(s) 63
PkrI GCNGC 8 cut(s) 50, 219, 259, 1692, 1789, 1832, 2002, 2428
PleI GAGTC 5 cut(s) 148, 239, 613, 1583, 2153
PmaCI CACGTG 1 cut(s) 2202
PmlI CACGTG 1 cut(s) 2202
PpsI GAGTC 5 cut(s) 148, 239, 613, 1583, 2153
Ppu21I YACGTR 1 cut(s) 2202
PscI ACATGT 1 cut(s) 1992
PshBI ATTAAT 1 cut(s) 1590
Psp124BI GAGCTC 1 cut(s) 126
Psp1406I AACGTT 1 cut(s) 1216
PspCI CACGTG 1 cut(s) 2202
PspFI CCCAGC 2 cut(s) 1444, 2023
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 6 cut(s) 164, 1408, 1821, 1857, 2022, 2475
PspPI GGNCC 6 cut(s) 162, 903, 2020, 2162, 2347, 2576
PspXI VCTCGAGB 1 cut(s) 125
PstI CTGCAG 1 cut(s) 59
PstNI CAGNNNCTG 1 cut(s) 54
PsuI RGATCY 1 cut(s) 156
RsaI GTAC 5 cut(s) 65, 557, 1408, 2475, 2559
RsaNI GTAC 5 cut(s) 64, 556, 1407, 2474, 2558
RseI CAYNNNNRTG 4 cut(s) 1089, 1343, 1825, 2200
SacI GAGCTC 1 cut(s) 126
SalI GTCGAC 1 cut(s) 142
SapI GCTCTTC 2 cut(s) 441, 2010
SatI GCNGC 8 cut(s) 49, 218, 258, 1691, 1788, 1831, 2001, 2427
Sau3AI GATC 6 cut(s) 156, 205, 668, 735, 2118, 2307
Sau96I GGNCC 6 cut(s) 162, 903, 2020, 2162, 2347, 2576
SchI GAGTC 5 cut(s) 149, 239, 614, 1584, 2154
ScrFI CCNGG 1 cut(s) 2471
SduI GDGCHC 2 cut(s) 126, 135
SfaNI GCATC 5 cut(s) 1347, 1453, 1815, 1918, 2170
SfcI CTRYAG 3 cut(s) 55, 498, 1151
Sfr274I CTCGAG 2 cut(s) 125, 179
SinI GGWCC 5 cut(s) 162, 903, 2020, 2162, 2576
SlaI CTCGAG 2 cut(s) 125, 179
SmiMI CAYNNNNRTG 4 cut(s) 1089, 1343, 1825, 2200
SmlI CTYRAG 4 cut(s) 125, 179, 881, 1260
SmoI CTYRAG 4 cut(s) 125, 179, 881, 1260
SphI GCATGC 1 cut(s) 80
SspMI CTAG 3 cut(s) 989, 2192, 2430
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 2469
StyI CCWWGG 1 cut(s) 1801
TaaI ACNGT 1 cut(s) 544
TaiI ACGT 4 cut(s) 1219, 1527, 1779, 2204
TauI GCSGC 2 cut(s) 220, 260
TscAI CASTG 3 cut(s) 825, 1875, 2233
TseFI GTSAC 2 cut(s) 683, 1628
TseI GCWGC 6 cut(s) 48, 1690, 1787, 1830, 2000, 2426
Tsp45I GTSAC 2 cut(s) 683, 1628
TspGWI ACGGA 3 cut(s) 82, 573, 2292
TspRI CASTG 3 cut(s) 825, 1875, 2233
VpaK11BI GGWCC 5 cut(s) 162, 903, 2020, 2162, 2576
VspI ATTAAT 1 cut(s) 1590
XagI CCTNNNNNAGG 2 cut(s) 1034, 2129
XapI RAATTY 6 cut(s) 592, 1025, 1169, 1903, 2233, 2384
XceI RCATGY 2 cut(s) 80, 1996
XcmI CCANNNNNNNNNTGG 1 cut(s) 1456
XhoI CTCGAG 2 cut(s) 125, 179
XmiI GTMKAC 1 cut(s) 143
XspI CTAG 3 cut(s) 989, 2192, 2430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.