MD04G1214700.v1.1

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000126
Physical Location & Seq
Reverse (-)
29784032 .. 29789429
5398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1214700.v1.1.491

Sequence Viewer

Length: 3069 bp
ATGAAGTACAACTCTGAAATCGAATCGAAAGCGGACGAGTTGGGGGGGCTTCAGAGGTTGATCGACGAGAAGGTTGAGGAGGTTATACAGAGTAAGAACCACTTGCACTCTCTCCAGTCATTGATTCAGGAATGTAGCAATGAGGTTATGGTGCAAGAGAAGAGATTGACGGAGGCTGAGGGTTTTGTTGTGGAGAAGAAGAGGGAGTGCGATTCGATTGAGAAGCGGGTTGAGCGGAGGCAGAAAAAATTGGATTGGGTTGAGAGAAGGGTGGAGGAGAAGTTGAAGTTGGCGGAGGAGAAGTCGAAATTGGCTGAGGTGAAGGCGGAGGAAGTGAGGGGGTTTGGAGAGGCGTTGGAGAAGTGTGTCGAAGAGATTGAGTTGAAGAAGAGGGAGTTGAATGAGATTATCGGGTCGATTGAGAAGCGCAAGAAGGAGTTCAATTCGATAGGGGAGCAGATTATGGGGGCGGAAAAGTTGATGAAGGTCCAGGAGGAGCAGCTGGGTTTTAAAGAAGAGAGAATTAAGGAGGCGCAAAGGGCGGTTGAACAATGTGACAAAGAGTTGAAATGGAGAGAGAAGCTGAAAACGGAGGCGGAAAGGGCGGTTGAAGAATGTGACAAGGAGTTGAAATGGAGAGAGAAGCTGAAAACGGAGGCGGAAAGGGCGGTTGAAGAACGAGACAAGGAGTTGAAGCTGAAAAAGGAGAAACTTGTTGTGATTGATAAGTCTTTAGCGGAGTGCTCGAATGCGCTGGAATCAAGAGAGAAAAGGATTAGGGAAATAGACTCGAAAAAAGATAAAGATTTGAGCTTGCGTGAGAAATCATTGGAGGAGTGGTCCTGTAGGCTTGAGGTTAAAGAGAGGGAAGTTAGATTGGGAGAGAGAATTGTCGAGTTAAAAGCTGGGGAACTCCAAAAGTTGAAAGAAGGCGAAAGGTGTTTGGAATCTATCTCGCAGGTACTTGAACAGAAAGAGAACCGACTTCAAGACCTTGACGAAGAGCTCAAATTGAAGCAGAAAGAACTTGATTTGATAAAAAAATCCACTGAAGAACGCGCCAAAAACTTGGAATTGAAAGAGAGGCAACTTGAAGATCAGGCCAAAGAGCTTGAACTGAAGCAGAAAGGATTTGATTCGATAAAAAAAATCTCCGAGGAACGCACCCAAAACCTGAAATCGAAAGAGAATACTAGTATTCTTGTTTCTCAAGTGAAAATTGAGCAATTGGAACATATCCCTGCCAACAATGCTGTTGTTCCTTCTACCTCAAGTAACCAGTCCGGCGTCAATATGGATGGTAGAGGCTTGCTGTTGTTCATAAATGAGCATTTGAAGAGATATGTTGTATTGGGTAGTGAAATCTCAGCTGTTCTTCAGGCGTCACCAGACCCGGCAAAATTAGTTTTGGATGCTATGCAAGGGTTTTACCATGCAAATTCAAGTGTGGACGAAAGCGAGTGTGATTTTGAGAGCATTACTAGGATGACTTGTAGCCTTCTGTTGGAGAATTTAAAGAGTGTCTCCCCCCAAATTAACCCTGAGGTGAGAGAGGAAGCAATTAAGTTGGCAGGTGATTGGAAGGTTAAAATGAGGACGGACAATTGCTTGGAGGTTTTGGGTTTTCTGCGGCTTGTTAGTACATATGGAATCACCTCGTTTTATGATGCGAAGGAGCTTCAAAGTCTTTGTGCGATTGTTGCTAAGGATGAACGTACAACTGAATTATCCTGGGCTTTTGGTATGACTGCCAGAATTGAGGCCCCGGAATCTTCACTGGCAAACAATGCAGGAACTTGTTCTTCTCTGAATCTTCAACTAACTGCCACCACAGATGCTACTCATTCGCAGGGGTTTTTAAATGAGCTTTTCAGTAGGGATTGTTTGATACAGACTGAAACTTGGGCTGCTTTTCAATCGTCATCAGAACCAGAAAAGTTTGTGATGGATGTGATGCAAACTTCTTTTTATAAATACTGCACAATAGAGGATGCTGGTTTTAAAGAAATTGTCATGGCAAAATACATTTCACTACTCGACATGCTGATGAAAATTAAACCGCATGTTGGACCTCATGTTAACATAGATGCTCTAAAGCTAGCAGTCAATTGGAAATCAAAAATTGGTGCCAGTACCCAACCCCTTGAGCTTTTGGGTTTTTTGCAGTTCATAGCTACATATGGACTGCTTTCCATGTTTAATAGAGAGACTTTAGTGTTTCTTGGAAGGATTTCTCATCAGAAGCAGGCGCTAGAAATATGTCAGACACTTGGTTTAGCTGATAAGATCCCTGATATCATTCGGGATCTTGTTGAAAAGAACCAACTAATTGAGGCTGTTCGATTAATTTGCCCGTTTAAGTTAATTGACAAGTTCCCACCAGTACCACTCTTGAAAGAATTTGTGGAGAATGCAAAGTTGTCGTGCATTCAAATGAGCAAGGGACATAAATTATTTTCTGAAAAGGATAAGTGTGTAAACAACCAGATTGCTGCTCTTAGAGTTGTGATTCAATGTATCAAAGATAGCAACCTCGAGTCCGAGTACCCTTCCAGGTTCATTGAAACACAAATAGCTTTTCTGGAAAAAATGAATAATCATCGCAGACGCCAAATGACATCTCCTCCCTCCAAGGTTGAACAACGATGGAAGAAAAATTCTCCGGTCTCCAAGGTTGAGGGACAAGAGCAGAAACAGTCTCCTGCCTCCAAAGTTGAGGGACGGGAGCAGAAAAAGTCTCCTTCCCCTAGAATTGAGGGACGAGAGCAGATCAAACCTCTTGCCTCCAATGTTGAACAAGGGGCGGAAAAGCCTTTTGCTTATACTGTTGAGGGACAAGAGCAGAAAAGATCTGTTCCCTCCCACGCTGAACCAGAAGAACACAGTAGAGGCAAGAAACGTAGACCCAACACTTTGCCTTGCTCGATTCAACCACCACAACAGCGTCGAAATATGTATCATCAGACATCTGCTTCACATTCTATTCATCACCTCCAATCACCCCAGACTATCGGAAGCCAGGTCCGTTGCCTGGGGAGAGTTTTTAAAGCCATGCCGAATCCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida

Protein Analysis

1023

Amino Acids

117.16

Weight (kDa)

6.71

Isoelectric Point (pI)

54.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 426 - 587 9.9e-33 Frigida-like protein
Frigida PF07899 607 - 872 2.4e-54 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1971
AarI CACCTGC 1 cut(s) 1562
Acc36I ACCTGC 2 cut(s) 949, 1562
AccB1I GGYRCC 1 cut(s) 2126
AccBSI CCGCTC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 2902
AccII CGCG 1 cut(s) 1059
AclWI GGATC 2 cut(s) 2281, 2313
AcsI RAATTY 4 cut(s) 1438, 1510, 2399, 2656
AcuI CTGAAG 4 cut(s) 35, 1071, 1139, 1361
AcyI GRCGYC 3 cut(s) 1287, 1382, 2608
AfaI GTAC 7 cut(s) 8, 963, 1642, 1717, 2134, 2385, 2546
AfiI CCNNNNNNNGG 3 cut(s) 1504, 2066, 3031
AhlI ACTAGT 1 cut(s) 1193
AjnI CCWGG 5 cut(s) 489, 1730, 2552, 3018, 3030
AjuI GAANNNNNNNTTGG 4 cut(s) 272, 293, 304, 325
Alw21I GWGCWC 2 cut(s) 746, 1008
Alw26I GTCTC 6 cut(s) 675, 1528, 2201, 2671, 2703, 2742
AlwI GGATC 2 cut(s) 2281, 2313
Ama87I CYCGRG 1 cut(s) 2534
AoxI GGCC 2 cut(s) 1101, 1761
ApeKI GCWGC 3 cut(s) 499, 1907, 2492
ApoI RAATTY 4 cut(s) 1438, 1510, 2399, 2656
AseI ATTAAT 1 cut(s) 2345
Asp700I GAANNNNTTC 3 cut(s) 437, 1798, 2230
AspLEI GCGC 5 cut(s) 429, 535, 754, 1061, 2251
AspS9I GGNCC 5 cut(s) 487, 840, 1762, 2069, 3022
AsuC2I CCSGG 2 cut(s) 1394, 1766
AsuHPI GGTGA 7 cut(s) 331, 1377, 1558, 1586, 1645, 2981, 2991
AsuNHI GCTAGC 1 cut(s) 2098
AvaI CYCGRG 1 cut(s) 2534
AvaII GGWCC 4 cut(s) 487, 840, 2069, 3022
AxyI CCTNAGG 1 cut(s) 1542
BanI GGYRCC 1 cut(s) 2126
BanII GRGCYC 1 cut(s) 1008
Bbv12I GWGCWC 2 cut(s) 746, 1008
BbvCI CCTCAGC 2 cut(s) 177, 315
BbvI GCAGC 3 cut(s) 511, 1894, 2479
BccI CCATC 3 cut(s) 1292, 1939, 2640
BcgI CGANNNNNNTGC 4 cut(s) 2331, 2365, 2403, 2437
BciT130I CCWGG 5 cut(s) 491, 1732, 2554, 3020, 3032
BcnI CCSGG 2 cut(s) 1394, 1766
BcoDI GTCTC 6 cut(s) 675, 1528, 2201, 2671, 2703, 2742
BcuI ACTAGT 1 cut(s) 1193
BfaI CTAG 5 cut(s) 1194, 1482, 2099, 2252, 2748
BfmI CTRYAG 1 cut(s) 844
BfoI RGCGCY 1 cut(s) 2252
BfuAI ACCTGC 2 cut(s) 949, 1562
BglII AGATCT 1 cut(s) 2849
BisI GCNGC 4 cut(s) 500, 1631, 1908, 2493
BlsI GCNGC 4 cut(s) 501, 1632, 1909, 2494
Bme1390I CCNGG 7 cut(s) 491, 1394, 1732, 1766, 2554, 3020, 3032
Bme18I GGWCC 4 cut(s) 487, 840, 2069, 3022
BmeT110I CYCGRG 1 cut(s) 2534
BmgT120I GGNCC 5 cut(s) 487, 840, 1762, 2069, 3022
BmiI GGNNCC 2 cut(s) 1764, 2128
BmrFI CCNGG 7 cut(s) 491, 1394, 1732, 1766, 2554, 3020, 3032
BmsI GCATC 6 cut(s) 1402, 1657, 1825, 1944, 1981, 2077
BmtI GCTAGC 1 cut(s) 2102
BpmI CTGGAG 1 cut(s) 98
Bpu10I CCTNAGC 3 cut(s) 177, 315, 1704
BpuEI CTTGAG 4 cut(s) 872, 1194, 1255, 2165
BpuMI CCSGG 2 cut(s) 1394, 1766
BsaHI GRCGYC 3 cut(s) 1287, 1382, 2608
BsaI GGTCTC 1 cut(s) 2671
BsaJI CCNNGG 6 cut(s) 1155, 1731, 1764, 2631, 2670, 3031
BsaWI WCCGGW 1 cut(s) 2662
BsaXI ACNNNNNCTCC 8 cut(s) 266, 287, 296, 317, 824, 854, 2608, 2638
Bsc4I CCNNNNNNNGG 3 cut(s) 1504, 2066, 3031
Bse1I ACTGG 5 cut(s) 115, 1279, 1782, 2130, 2381
Bse21I CCTNAGG 1 cut(s) 1542
Bse3DI GCAATG 1 cut(s) 145
BseBI CCWGG 5 cut(s) 491, 1732, 2554, 3020, 3032
BseDI CCNNGG 6 cut(s) 1155, 1731, 1764, 2631, 2670, 3031
BseGI GGATG 6 cut(s) 1303, 1417, 1491, 1714, 1954, 1996
BseLI CCNNNNNNNGG 3 cut(s) 1504, 2066, 3031
BseMI GCAATG 1 cut(s) 145
BseMII CTCAG 4 cut(s) 168, 306, 1380, 1533
BseNI ACTGG 5 cut(s) 115, 1279, 1782, 2130, 2381
BseRI GAGGAG 6 cut(s) 92, 290, 311, 509, 848, 2613
BseXI GCAGC 3 cut(s) 511, 1894, 2479
BseYI CCCAGC 2 cut(s) 502, 905
BsgI GTGCAG 1 cut(s) 1963
Bsh1236I CGCG 1 cut(s) 1059
BshFI GGCC 2 cut(s) 1103, 1763
BshNI GGYRCC 1 cut(s) 2126
BsiHKAI GWGCWC 2 cut(s) 746, 1008
BsiHKCI CYCGRG 1 cut(s) 2534
BsiSI CCGG 4 cut(s) 1284, 1394, 1766, 2663
BslFI GGGAC 5 cut(s) 2457, 2694, 2733, 2772, 2847
BslI CCNNNNNNNGG 3 cut(s) 1504, 2066, 3031
BsmAI GTCTC 6 cut(s) 675, 1528, 2201, 2671, 2703, 2742
BsmFI GGGAC 5 cut(s) 2457, 2694, 2733, 2772, 2847
BsmI GAATGC 3 cut(s) 754, 2416, 2427
BsnI GGCC 2 cut(s) 1103, 1763
Bso31I GGTCTC 1 cut(s) 2671
BsoBI CYCGRG 1 cut(s) 2534
Bsp1286I GDGCHC 2 cut(s) 746, 1008
Bsp143I GATC 6 cut(s) 60, 1096, 2286, 2305, 2769, 2849
BspANI GGCC 2 cut(s) 1103, 1763
BspCNI CTCAG 4 cut(s) 169, 307, 1379, 1534
BspFNI CGCG 1 cut(s) 1059
BspLI GGNNCC 2 cut(s) 1764, 2128
BspMI ACCTGC 2 cut(s) 949, 1562
BspOI GCTAGC 1 cut(s) 2102
BspPI GGATC 2 cut(s) 2281, 2313
BspQI GCTCTTC 1 cut(s) 996
BspT107I GGYRCC 1 cut(s) 2126
BspTNI GGTCTC 1 cut(s) 2671
BsrBI CCGCTC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 145
BsrI ACTGG 5 cut(s) 115, 1279, 1782, 2130, 2381
BssECI CCNNGG 6 cut(s) 1155, 1731, 1764, 2631, 2670, 3031
BssMI GATC 6 cut(s) 60, 1096, 2286, 2305, 2769, 2849
BssNI GRCGYC 3 cut(s) 1287, 1382, 2608
BssT1I CCWWGG 2 cut(s) 2631, 2670
Bst2UI CCWGG 5 cut(s) 491, 1732, 2554, 3020, 3032
Bst4CI ACNGT 3 cut(s) 2697, 2827, 2885
Bst6I CTCTTC 7 cut(s) 155, 194, 366, 383, 510, 996, 1331
BstACI GRCGYC 3 cut(s) 1287, 1382, 2608
BstAPI GCANNNNNTGC 1 cut(s) 1787
BstC8I GCNNGC 4 cut(s) 815, 1310, 2100, 2247
BstDEI CTNAG 6 cut(s) 177, 315, 1366, 1542, 1704, 2498
BstF5I GGATG 6 cut(s) 1303, 1417, 1491, 1714, 1954, 1996
BstFNI CGCG 1 cut(s) 1059
BstH2I RGCGCY 1 cut(s) 2252
BstHHI GCGC 5 cut(s) 429, 535, 754, 1061, 2251
BstKTI GATC 6 cut(s) 63, 1099, 2289, 2308, 2772, 2852
BstMAI GTCTC 6 cut(s) 675, 1528, 2201, 2671, 2703, 2742
BstMBI GATC 6 cut(s) 60, 1096, 2286, 2305, 2769, 2849
BstMWI GCNNNNNNNGC 7 cut(s) 232, 539, 602, 665, 1250, 1700, 1787
BstNI CCWGG 5 cut(s) 491, 1732, 2554, 3020, 3032
BstNSI RCATGY 2 cut(s) 2044, 2066
BstSCI CCNGG 7 cut(s) 489, 1392, 1730, 1764, 2552, 3018, 3030
BstSFI CTRYAG 1 cut(s) 844
BstUI CGCG 1 cut(s) 1059
BstV1I GCAGC 3 cut(s) 511, 1894, 2479
BstX2I RGATCY 3 cut(s) 2286, 2305, 2849
BstXI CCANNNNNNTGG 1 cut(s) 1069
BstYI RGATCY 3 cut(s) 2286, 2305, 2849
Bsu36I CCTNAGG 1 cut(s) 1542
BsuRI GGCC 2 cut(s) 1103, 1763
BtgZI GCGATG 1 cut(s) 2585
BtsCI GGATG 6 cut(s) 1303, 1417, 1491, 1714, 1954, 1996
BtsIMutI CAGTG 2 cut(s) 1047, 1775
BveI ACCTGC 2 cut(s) 949, 1562
Cac8I GCNNGC 4 cut(s) 815, 1310, 2100, 2247
CfoI GCGC 5 cut(s) 429, 535, 754, 1061, 2251
Cfr13I GGNCC 5 cut(s) 487, 840, 1762, 2069, 3022
CseI GACGC 4 cut(s) 1276, 1371, 2616, 2933
Csp6I GTAC 7 cut(s) 7, 962, 1641, 1716, 2133, 2384, 2545
CviAII CATG 7 cut(s) 1433, 2014, 2041, 2063, 2075, 2194, 3052
CviQI GTAC 7 cut(s) 7, 962, 1641, 1716, 2133, 2384, 2545
DdeI CTNAG 6 cut(s) 177, 315, 1366, 1542, 1704, 2498
DpnI GATC 6 cut(s) 62, 1098, 2288, 2307, 2771, 2851
DpnII GATC 6 cut(s) 60, 1096, 2286, 2305, 2769, 2849
DraI TTTAAA 5 cut(s) 511, 1515, 1860, 2002, 3046
Eam1104I CTCTTC 7 cut(s) 155, 194, 366, 383, 510, 996, 1331
EarI CTCTTC 7 cut(s) 155, 194, 366, 383, 510, 996, 1331
EciI GGCGGA 6 cut(s) 308, 341, 485, 611, 674, 2819
Ecl136II GAGCTC 1 cut(s) 1006
Eco130I CCWWGG 2 cut(s) 2631, 2670
Eco24I GRGCYC 1 cut(s) 1008
Eco31I GGTCTC 1 cut(s) 2671
Eco32I GATATC 1 cut(s) 2296
Eco47I GGWCC 4 cut(s) 487, 840, 2069, 3022
Eco53kI GAGCTC 1 cut(s) 1006
Eco57I CTGAAG 4 cut(s) 35, 1071, 1139, 1361
Eco81I CCTNAGG 1 cut(s) 1542
Eco88I CYCGRG 1 cut(s) 2534
EcoICRI GAGCTC 1 cut(s) 1006
EcoO109I RGGNCCY 1 cut(s) 1762
EcoRII CCWGG 5 cut(s) 489, 1730, 2552, 3018, 3030
EcoRV GATATC 1 cut(s) 2296
EcoT14I CCWWGG 2 cut(s) 2631, 2670
EcoT38I GRGCYC 1 cut(s) 1008
ErhI CCWWGG 2 cut(s) 2631, 2670
FaeI CATG 7 cut(s) 1436, 2017, 2044, 2066, 2078, 2197, 3055
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 5 cut(s) 2457, 2694, 2733, 2772, 2847
FatI CATG 7 cut(s) 1432, 2013, 2040, 2062, 2074, 2193, 3051
FauI CCCGC 1 cut(s) 219
FauNDI CATATG 2 cut(s) 1645, 2179
FblI GTMKAC 1 cut(s) 2902
Fnu4HI GCNGC 4 cut(s) 500, 1631, 1908, 2493
FokI GGATG 6 cut(s) 1310, 1424, 1498, 1721, 1961, 2003
FriOI GRGCYC 1 cut(s) 1008
Fsp4HI GCNGC 4 cut(s) 500, 1631, 1908, 2493
FspBI CTAG 5 cut(s) 1194, 1482, 2099, 2252, 2748
GlaI GCGC 5 cut(s) 428, 534, 753, 1060, 2250
GluI GCNGC 4 cut(s) 500, 1631, 1908, 2493
GsaI CCCAGC 2 cut(s) 506, 909
GsuI CTGGAG 1 cut(s) 98
HaeII RGCGCY 1 cut(s) 2252
HaeIII GGCC 2 cut(s) 1103, 1763
HapII CCGG 4 cut(s) 1284, 1394, 1766, 2663
HgaI GACGC 4 cut(s) 1276, 1371, 2616, 2933
HhaI GCGC 5 cut(s) 429, 535, 754, 1061, 2251
Hin1I GRCGYC 3 cut(s) 1287, 1382, 2608
Hin1II CATG 7 cut(s) 1436, 2017, 2044, 2066, 2078, 2197, 3055
Hin6I GCGC 5 cut(s) 427, 533, 752, 1059, 2249
HinP1I GCGC 5 cut(s) 427, 533, 752, 1059, 2249
HincII GTYRAC 1 cut(s) 2080
HindII GTYRAC 1 cut(s) 2080
HpaI GTTAAC 1 cut(s) 2080
HpaII CCGG 4 cut(s) 1284, 1394, 1766, 2663
HphI GGTGA 7 cut(s) 331, 1377, 1558, 1586, 1645, 2981, 2991
Hpy166II GTNNAC 4 cut(s) 1450, 2080, 2479, 2903
Hpy188III TCNNGA 7 cut(s) 128, 762, 989, 2303, 2392, 2582, 3062
Hpy8I GTNNAC 4 cut(s) 1450, 2080, 2479, 2903
Hpy99I CGWCG 2 cut(s) 68, 2949
HpyCH4III ACNGT 3 cut(s) 2697, 2827, 2885
HpyCH4IV ACGT 2 cut(s) 1714, 2899
HpyF10VI GCNNNNNNNGC 7 cut(s) 232, 539, 602, 665, 1250, 1700, 1787
HpyF3I CTNAG 6 cut(s) 177, 315, 1366, 1542, 1704, 2498
HpySE526I ACGT 2 cut(s) 1714, 2899
Hsp92I GRCGYC 3 cut(s) 1287, 1382, 2608
Hsp92II CATG 7 cut(s) 1436, 2017, 2044, 2066, 2078, 2197, 3055
HspAI GCGC 5 cut(s) 427, 533, 752, 1059, 2249
KspAI GTTAAC 1 cut(s) 2080
Kzo9I GATC 6 cut(s) 60, 1096, 2286, 2305, 2769, 2849
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 4 cut(s) 454, 496, 1675, 2725
Lsp1109I GCAGC 3 cut(s) 511, 1894, 2479
LweI GCATC 6 cut(s) 1402, 1657, 1825, 1944, 1981, 2077
MaeI CTAG 5 cut(s) 1194, 1482, 2099, 2252, 2748
MaeII ACGT 2 cut(s) 1714, 2899
MaeIII GTNAC 4 cut(s) 554, 617, 1274, 1383
MalI GATC 6 cut(s) 62, 1098, 2288, 2307, 2771, 2851
MbiI CCGCTC 1 cut(s) 235
MboI GATC 6 cut(s) 60, 1096, 2286, 2305, 2769, 2849
MfeI CAATTG 3 cut(s) 1226, 1603, 2107
MflI RGATCY 3 cut(s) 2286, 2305, 2849
MhlI GDGCHC 2 cut(s) 746, 1008
MlyI GAGTC 2 cut(s) 782, 2546
MmeI TCCRAC 3 cut(s) 336, 1485, 2047
MroXI GAANNNNTTC 3 cut(s) 437, 1798, 2230
MslI CAYNNNNRTG 2 cut(s) 2045, 2432
MspA1I CMGCKG 2 cut(s) 502, 1370
MspI CCGG 4 cut(s) 1284, 1394, 1766, 2663
MspR9I CCNGG 7 cut(s) 491, 1394, 1732, 1766, 2554, 3020, 3032
MunI CAATTG 3 cut(s) 1226, 1603, 2107
Mva1269I GAATGC 3 cut(s) 754, 2416, 2427
MvaI CCWGG 5 cut(s) 491, 1732, 2554, 3020, 3032
MvnI CGCG 1 cut(s) 1059
MwoI GCNNNNNNNGC 7 cut(s) 232, 539, 602, 665, 1250, 1700, 1787
NciI CCSGG 2 cut(s) 1394, 1766
NdeI CATATG 2 cut(s) 1645, 2179
NdeII GATC 6 cut(s) 60, 1096, 2286, 2305, 2769, 2849
NheI GCTAGC 1 cut(s) 2098
NlaIII CATG 7 cut(s) 1436, 2017, 2044, 2066, 2078, 2197, 3055
NlaIV GGNNCC 2 cut(s) 1764, 2128
NmuCI GTSAC 3 cut(s) 554, 617, 1383
NspI RCATGY 2 cut(s) 2044, 2066
PaeR7I CTCGAG 1 cut(s) 2534
PaqCI CACCTGC 1 cut(s) 1562
PciSI GCTCTTC 1 cut(s) 996
PctI GAATGC 3 cut(s) 754, 2416, 2427
PdmI GAANNNNTTC 3 cut(s) 437, 1798, 2230
PfoI TCCNGGA 1 cut(s) 489
PkrI GCNGC 4 cut(s) 501, 1632, 1909, 2494
PleI GAGTC 2 cut(s) 782, 2545
PpsI GAGTC 2 cut(s) 782, 2545
PshBI ATTAAT 1 cut(s) 2345
PsiI TTATAA 1 cut(s) 1971
Psp124BI GAGCTC 1 cut(s) 1008
Psp6I CCWGG 5 cut(s) 489, 1730, 2552, 3018, 3030
PspFI CCCAGC 2 cut(s) 502, 905
PspGI CCWGG 5 cut(s) 489, 1730, 2552, 3018, 3030
PspN4I GGNNCC 2 cut(s) 1764, 2128
PspPI GGNCC 5 cut(s) 487, 840, 1762, 2069, 3022
PspXI VCTCGAGB 1 cut(s) 2534
PsuI RGATCY 3 cut(s) 2286, 2305, 2849
PvuII CAGCTG 2 cut(s) 502, 1370
RsaI GTAC 7 cut(s) 8, 963, 1642, 1717, 2134, 2385, 2546
RsaNI GTAC 7 cut(s) 7, 962, 1641, 1716, 2133, 2384, 2545
RseI CAYNNNNRTG 2 cut(s) 2045, 2432
SacI GAGCTC 1 cut(s) 1008
SapI GCTCTTC 1 cut(s) 996
SatI GCNGC 4 cut(s) 500, 1631, 1908, 2493
Sau3AI GATC 6 cut(s) 60, 1096, 2286, 2305, 2769, 2849
Sau96I GGNCC 5 cut(s) 487, 840, 1762, 2069, 3022
SchI GAGTC 2 cut(s) 782, 2546
ScrFI CCNGG 7 cut(s) 491, 1394, 1732, 1766, 2554, 3020, 3032
SduI GDGCHC 2 cut(s) 746, 1008
SfaNI GCATC 6 cut(s) 1402, 1657, 1825, 1944, 1981, 2077
SfcI CTRYAG 1 cut(s) 844
Sfr274I CTCGAG 1 cut(s) 2534
SinI GGWCC 4 cut(s) 487, 840, 2069, 3022
SlaI CTCGAG 1 cut(s) 2534
SmiMI CAYNNNNRTG 2 cut(s) 2045, 2432
SmlI CTYRAG 5 cut(s) 851, 1209, 1270, 2144, 2534
SmoI CTYRAG 5 cut(s) 851, 1209, 1270, 2144, 2534
SpeI ACTAGT 1 cut(s) 1193
SspMI CTAG 5 cut(s) 1194, 1482, 2099, 2252, 2748
SstI GAGCTC 1 cut(s) 1008
StyD4I CCNGG 7 cut(s) 489, 1392, 1730, 1764, 2552, 3018, 3030
StyI CCWWGG 2 cut(s) 2631, 2670
TaaI ACNGT 3 cut(s) 2697, 2827, 2885
TaiI ACGT 2 cut(s) 1717, 2902
TatI WGTACW 2 cut(s) 6, 1640
TauI GCSGC 1 cut(s) 1633
TscAI CASTG 2 cut(s) 1054, 1782
TseFI GTSAC 3 cut(s) 554, 617, 1383
TseI GCWGC 3 cut(s) 499, 1907, 2492
Tsp45I GTSAC 3 cut(s) 554, 617, 1383
TspDTI ATGAA 9 cut(s) 17, 497, 1309, 1725, 2063, 2158, 2548, 2606, 2975
TspGWI ACGGA 5 cut(s) 185, 605, 668, 1613, 3014
TspRI CASTG 2 cut(s) 1054, 1782
VpaK11BI GGWCC 4 cut(s) 487, 840, 2069, 3022
VspI ATTAAT 1 cut(s) 2345
XapI RAATTY 4 cut(s) 1438, 1510, 2399, 2656
XceI RCATGY 2 cut(s) 2044, 2066
XhoI CTCGAG 1 cut(s) 2534
XmiI GTMKAC 1 cut(s) 2902
XmnI GAANNNNTTC 3 cut(s) 437, 1798, 2230
XspI CTAG 5 cut(s) 1194, 1482, 2099, 2252, 2748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.