MD12G1013700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000126
Physical Location & Seq
Reverse (-)
1429778 .. 1434170
4393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1013700.v1.1.491

Sequence Viewer

Length: 2583 bp
ATGGAAGAGATAGCTTCGAAGTTGAAGCTGTCGGAGACGAAGCAGCGGAGCATTGGCAAGGCCCAGGAGACCCTGCGCGCCCAGGTGGATTCCATGCTCGCATTCACCCTCAACTGGAAGGACCTCGACGACCACTTCGACTCTGTCAGGAACTCGCTATACGCCGCATTCGTAGGGCTCCTGGAGCGGGAGAAGGAGATTGGGTCCCGATTCGCGGACTTGGAAGTGAAAGAGAAGGAATTTGACTCGGCGATGGCGGTGAAAGGCAGGGAATTGCAGGCCATTGTGCGGGAGGTTGAAGAAGGTAAGTTACAGTTGCAATCTATTAAGTTATCTTTCGAAAAGTATTCGGTTGAAGAAGGTAGATTAGGAAAAGTACAGAAATTGGTTAAGGGGAAGGAGATGGAGTATTATAATATGCAGCAACGGATGAAAGAATTGTCGAAAGAGATCGAGGCGAAGGAGAAGCAGTTGAATGTGGTTAGGGGTAAGGTTGTGGAGCAATGGAAAGAGTTTGATTCGAGAGACGACGAAATTAGAGCTCAGCAAGCATTGATTGAGGAATACGACAGAGAGGTGCAGTCGAAAGAGCAGAAACTCGGTTTGATTGAGAAGGATTTGTGTGAGCTTGAGAAGTCAGTGGGGGAGTGGGACTGTAAAGTTGAATTGAAACAGTTGGAACTTAAAAGAAGTGTTCAGAAGGCCGAAAAACAATTTGAATCAAAATCTGAGGAGCTTAATTTGATTGGGAGGAGGGTCAATGATTGCCTCAATGATCTTCAGTTGAAAGAAAAGAAGTTGGATTCCCGTGAAAAGCATTTGGATTCGCTTGACAAGTCGATACAGGAATGTGGGAGGCGGCTTGAACAAAAGGCCAAAGAGCTTGAACTGAAGCAACAAGAATTTGAAAAGTCTAAAGAAGAACATATCCGAACAAATAACTCGAAGGAGAAGACTGATATACTTCATACTCAAGTGAAGGTGGAACAATCGGGACGTTTCCCTGATAACATTTCCGTCCCTCCTTTTTGTGCAACCAATCATTCAAGAATCATTAGGGATGGTAGGAGCTTGCAGTTTTTCATGAATGAGTATTTTAGAAGAACAGATTCAGTCTCTGTTGAAGTTTCAGCCTGTCTTCAATTGTCATCAGACCCAGCAAAATTAGTTCTTGATGCAATGCAAGGGTTTTACCCTTCAAGTTCGACTGTGGAAAACAGGGAATTCGATTTTGATTTGAGTGTAATTAGAAGGAGCTGTATTCATTTGTTAAAGGAGGTAAAGAGAGTCTCGCCAAAAATTAGTGATCAGGTGAGAGCAGAAGCGAATAAGTTGGCGGGTGAATGGAGAGCTAGGATGACTGTGGCAGCCGAGAATTGGTTGGAGGTTTTGGGTTTTTTGTGGCTTGTTACTGCATATGACTTGACCTCTATTTCTGATAGGGAGGAGCTTCAATCTCTTCTCAGTATAGCTGCTAATCACGAAAAACAGTCAGATGAATTGTATGGGATCCTTGACACGGAAGATAACACACCTGCGAGCATCAACATTTGTTCCTCTATCAAAACTGAGAAACCAGAAACTTCACTGGGAAAGAATGCCGCAACTTGTTCTTCTCCAAATCTTCAACTAACTGCCACCTCAGATGCTAGGAATTCGCAGGGGTTTCCAACTTGTTCTTCTCCAAATATTCAACTAGCCACGACAGATGCTAGGAATTTACAGCAGTTATCAAATGAGCAGTTTAGTGGGAATCGCTCAATACAGAATGAATTTTTACAGATTGCCAAGTTGTTTTCTGCGTTAGATGGAGATGTGTTAGTAGATTCTCTTGTGTCTATTTCTCAGAATAAACAGGCCCTAGAATATATTCGGAGTTCTGGTTTTGGAGATAAGATCCCTGATGCTATTCCGACTCTTATTGAAAGGAGGCAACGGATTGAGGCCGTTAGAGCAATTTGCACCTTCAACTTAATTGACAAGTTTCCCCCAGTACCCCTCTTAAAAGAACACGTGGATACTGGGATGAACTCTTTCCAGTCACTTTCCTCGAAAACGAAACTAGTTGGTGAAAAGATTAAGTTTGCAGACTACTATATGGCTGATCTAAGAGCCGTGTTTCAATGCATTAAAGATTACAACCTAGAGTCAGAATACCCGCCCTCGGTCATTGAAACAAAACTAGCTAAGATGCAGAACCACAAGGATAAGTTGAAACACACGGAGAACTGGAGAAGTTTGGCGCCATGTCACAAGGTTGAACAAAAGCAGAAAGGAGGGAGGAAACGCTCCGCCAAGTTTCAAGCACAACAGCTTAAATTTAAACGTCAGCGGGCAGCAGTATCAGCTCCCGCACCACATCCAATACCAAATCCCACCCCTGGATATGCACTGCCAAATCCGTCATATGTGATGTCTCAAAATGGTGGACACCCTTGGCAATTCGGTATGGCTGGCAGCAACGTAGACATTTCGAAAGGCATTTGTTACCATTGCAACGGGTTAGGGCATTGGAAACGCAATTGTCCCAAGTATTTATCCGAAAAGAGGAACGGAGATGTGATCAAATCACAGATGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida TEX13

Protein Analysis

861

Amino Acids

98.11

Weight (kDa)

7.97

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 354 - 509 1.3e-27 Frigida-like protein
Frigida PF07899 598 - 740 9.3e-28 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AarI CACCTGC 1 cut(s) 1543
Acc36I ACCTGC 1 cut(s) 1543
AccB1I GGYRCC 1 cut(s) 2242
AccBSI CCGCTC 1 cut(s) 187
AccI GTMKAC 1 cut(s) 2466
AccII CGCG 2 cut(s) 78, 215
AclWI GGATC 3 cut(s) 1504, 1517, 1891
AcsI RAATTY 7 cut(s) 239, 902, 1223, 1654, 1717, 1772, 2318
AcuI CTGAAG 2 cut(s) 764, 911
AcvI CACGTG 1 cut(s) 2014
AcyI GRCGYC 1 cut(s) 2243
AfaI GTAC 2 cut(s) 378, 1995
AfiI CCNNNNNNNGG 9 cut(s) 114, 187, 214, 288, 1377, 1519, 2164, 2381, 2547
AflIII ACRYGT 1 cut(s) 2011
AhlI ACTAGT 1 cut(s) 2062
AjnI CCWGG 4 cut(s) 63, 81, 180, 2380
AjuI GAANNNNNNNTTGG 2 cut(s) 1663, 1695
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 6 cut(s) 29, 62, 519, 1120, 1294, 2421
AlwI GGATC 3 cut(s) 1504, 1517, 1891
AlwNI CAGNNNCTG 1 cut(s) 1118
AoxI GGCC 6 cut(s) 60, 279, 702, 873, 1857, 1944
ApeKI GCWGC 6 cut(s) 43, 421, 1367, 1472, 2336, 2457
ApoI RAATTY 7 cut(s) 239, 902, 1223, 1654, 1717, 1772, 2318
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 3 cut(s) 1108, 1869, 2033
AspLEI GCGC 3 cut(s) 78, 80, 2245
AspS9I GGNCC 4 cut(s) 61, 121, 204, 1858
AsuHPI GGTGA 5 cut(s) 97, 271, 1324, 1352, 2081
AsuII TTCGAA 3 cut(s) 17, 339, 2474
AvaII GGWCC 2 cut(s) 121, 204
BamHI GGATCC 1 cut(s) 1509
BanI GGYRCC 1 cut(s) 2242
BanII GRGCYC 2 cut(s) 180, 544
BarI GAAGNNNNNNTAC 2 cut(s) 294, 326
BbrPI CACGTG 1 cut(s) 2014
BbsI GAAGAC 2 cut(s) 959, 1130
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 6 cut(s) 55, 433, 1379, 1459, 2348, 2469
BccI CCATC 4 cut(s) 247, 397, 1055, 1802
BceAI ACGGC 2 cut(s) 1931, 2099
BciT130I CCWGG 4 cut(s) 65, 83, 182, 2382
BciVI GTATCC 1 cut(s) 2011
BclI TGATCA 2 cut(s) 1306, 2562
BcoDI GTCTC 6 cut(s) 29, 62, 519, 1120, 1294, 2421
BcuI ACTAGT 1 cut(s) 2062
BfaI CTAG 8 cut(s) 1353, 1650, 1697, 1713, 1862, 2063, 2144, 2183
BfoI RGCGCY 1 cut(s) 2246
BfuAI ACCTGC 1 cut(s) 1543
BfuI GTATCC 1 cut(s) 2011
BisI GCNGC 9 cut(s) 44, 165, 422, 860, 1368, 1473, 1602, 2337, 2458
BlpI GCTNAGC 1 cut(s) 543
BlsI GCNGC 9 cut(s) 45, 166, 423, 861, 1369, 1474, 1603, 2338, 2459
Bme1390I CCNGG 4 cut(s) 65, 83, 182, 2382
Bme18I GGWCC 2 cut(s) 121, 204
BmgT120I GGNCC 4 cut(s) 61, 121, 204, 1858
BmiI GGNNCC 5 cut(s) 179, 205, 206, 1511, 2244
BmrFI CCNGG 4 cut(s) 65, 83, 182, 2382
BmrI ACTGGG 3 cut(s) 1598, 1985, 2031
BmsI GCATC 7 cut(s) 1165, 1551, 1636, 1699, 1894, 2181, 2565
BmuI ACTGGG 3 cut(s) 1598, 1985, 2031
BpiI GAAGAC 2 cut(s) 959, 1130
BpmI CTGGAG 2 cut(s) 203, 2251
Bpu1102I GCTNAGC 1 cut(s) 543
Bpu14I TTCGAA 3 cut(s) 17, 339, 2474
BpuEI CTTGAG 2 cut(s) 650, 957
BsaAI YACGTR 1 cut(s) 2014
BsaHI GRCGYC 1 cut(s) 2243
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 5 cut(s) 63, 81, 2163, 2380, 2435
BsaXI ACNNNNNCTCC 8 cut(s) 188, 218, 392, 422, 455, 485, 1867, 1897
Bsc4I CCNNNNNNNGG 9 cut(s) 114, 187, 214, 288, 1377, 1519, 2164, 2381, 2547
Bse1I ACTGG 6 cut(s) 119, 1593, 1991, 2026, 2038, 2234
Bse3DI GCAATG 3 cut(s) 509, 1185, 2491
BseBI CCWGG 4 cut(s) 65, 83, 182, 2382
BseDI CCNNGG 5 cut(s) 63, 81, 2163, 2380, 2435
BseGI GGATG 5 cut(s) 435, 1066, 1362, 2031, 2359
BseLI CCNNNNNNNGG 9 cut(s) 114, 187, 214, 288, 1377, 1519, 2164, 2381, 2547
BseMI GCAATG 3 cut(s) 509, 1185, 2491
BseMII CTCAG 6 cut(s) 557, 720, 1477, 1560, 1656, 1859
BseNI ACTGG 6 cut(s) 119, 1593, 1991, 2026, 2038, 2234
BsePI GCGCGC 1 cut(s) 76
BseRI GAGGAG 3 cut(s) 746, 766, 1460
BseXI GCAGC 6 cut(s) 55, 433, 1379, 1459, 2348, 2469
BseYI CCCAGC 1 cut(s) 1156
BsgI GTGCAG 1 cut(s) 599
Bsh1236I CGCG 2 cut(s) 78, 215
BshFI GGCC 6 cut(s) 62, 281, 704, 875, 1859, 1946
BshNI GGYRCC 1 cut(s) 2242
BsiHKAI GWGCWC 1 cut(s) 544
BslFI GGGAC 5 cut(s) 190, 665, 1004, 1008, 2511
BslI CCNNNNNNNGG 9 cut(s) 114, 187, 214, 288, 1377, 1519, 2164, 2381, 2547
BsmAI GTCTC 6 cut(s) 29, 62, 519, 1120, 1294, 2421
BsmBI CGTCTC 2 cut(s) 29, 519
BsmFI GGGAC 5 cut(s) 190, 665, 1004, 1008, 2511
BsmI GAATGC 3 cut(s) 101, 167, 1603
BsnI GGCC 6 cut(s) 62, 281, 704, 875, 1859, 1946
Bso31I GGTCTC 1 cut(s) 62
Bsp119I TTCGAA 3 cut(s) 17, 339, 2474
Bsp1286I GDGCHC 2 cut(s) 180, 544
Bsp143I GATC 7 cut(s) 450, 775, 1306, 1509, 1896, 2104, 2562
Bsp1720I GCTNAGC 1 cut(s) 543
BspANI GGCC 6 cut(s) 62, 281, 704, 875, 1859, 1946
BspCNI CTCAG 6 cut(s) 556, 721, 1476, 1561, 1655, 1858
BspFNI CGCG 2 cut(s) 78, 215
BspHI TCATGA 1 cut(s) 1083
BspLI GGNNCC 5 cut(s) 179, 205, 206, 1511, 2244
BspMI ACCTGC 1 cut(s) 1543
BspPI GGATC 3 cut(s) 1504, 1517, 1891
BspT104I TTCGAA 3 cut(s) 17, 339, 2474
BspT107I GGYRCC 1 cut(s) 2242
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 187
BsrDI GCAATG 3 cut(s) 509, 1185, 2491
BsrI ACTGG 6 cut(s) 119, 1593, 1991, 2026, 2038, 2234
BssECI CCNNGG 5 cut(s) 63, 81, 2163, 2380, 2435
BssHII GCGCGC 1 cut(s) 76
BssMI GATC 7 cut(s) 450, 775, 1306, 1509, 1896, 2104, 2562
BssNI GRCGYC 1 cut(s) 2243
BssT1I CCWWGG 1 cut(s) 2435
Bst2UI CCWGG 4 cut(s) 65, 83, 182, 2382
Bst4CI ACNGT 6 cut(s) 315, 656, 675, 1210, 1363, 1491
Bst6I CTCTTC 1 cut(s) 1464
BstACI GRCGYC 1 cut(s) 2243
BstBAI YACGTR 1 cut(s) 2014
BstBI TTCGAA 3 cut(s) 17, 339, 2474
BstC8I GCNNGC 8 cut(s) 78, 99, 279, 549, 1073, 1540, 2334, 2455
BstDEI CTNAG 8 cut(s) 543, 729, 1463, 1569, 1642, 1845, 2108, 2187
BstF5I GGATG 5 cut(s) 435, 1066, 1362, 2031, 2359
BstFNI CGCG 2 cut(s) 78, 215
BstH2I RGCGCY 1 cut(s) 2246
BstHHI GCGC 3 cut(s) 78, 80, 2245
BstKTI GATC 7 cut(s) 453, 778, 1309, 1512, 1899, 2107, 2565
BstMAI GTCTC 6 cut(s) 29, 62, 519, 1120, 1294, 2421
BstMBI GATC 7 cut(s) 450, 775, 1306, 1509, 1896, 2104, 2562
BstMWI GCNNNNNNNGC 4 cut(s) 184, 548, 1952, 2345
BstNI CCWGG 4 cut(s) 65, 83, 182, 2382
BstSCI CCNGG 4 cut(s) 63, 81, 180, 2380
BstUI CGCG 2 cut(s) 78, 215
BstV1I GCAGC 6 cut(s) 55, 433, 1379, 1459, 2348, 2469
BstV2I GAAGAC 2 cut(s) 959, 1130
BstX2I RGATCY 2 cut(s) 1509, 1896
BstYI RGATCY 2 cut(s) 1509, 1896
BsuI GTATCC 1 cut(s) 2011
BsuRI GGCC 6 cut(s) 62, 281, 704, 875, 1859, 1946
BtgZI GCGATG 1 cut(s) 266
BtsCI GGATG 5 cut(s) 435, 1066, 1362, 2031, 2359
BtsI GCAGTG 1 cut(s) 2390
BtsIMutI CAGTG 3 cut(s) 645, 1586, 2390
BveI ACCTGC 1 cut(s) 1543
Cac8I GCNNGC 8 cut(s) 78, 99, 279, 549, 1073, 1540, 2334, 2455
CaiI CAGNNNCTG 1 cut(s) 1118
CciI TCATGA 1 cut(s) 1083
CfoI GCGC 3 cut(s) 78, 80, 2245
Cfr13I GGNCC 4 cut(s) 61, 121, 204, 1858
Csp6I GTAC 2 cut(s) 377, 1994
CviAII CATG 3 cut(s) 94, 1084, 2247
CviQI GTAC 2 cut(s) 377, 1994
DdeI CTNAG 8 cut(s) 543, 729, 1463, 1569, 1642, 1845, 2108, 2187
DinI GGCGCC 1 cut(s) 2244
DpnI GATC 7 cut(s) 452, 777, 1308, 1511, 1898, 2106, 2564
DpnII GATC 7 cut(s) 450, 775, 1306, 1509, 1896, 2104, 2562
DraI TTTAAA 1 cut(s) 2323
Eam1104I CTCTTC 1 cut(s) 1464
EarI CTCTTC 1 cut(s) 1464
EciI GGCGGA 1 cut(s) 2281
Ecl136II GAGCTC 1 cut(s) 542
Eco130I CCWWGG 1 cut(s) 2435
Eco24I GRGCYC 2 cut(s) 180, 544
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 2 cut(s) 121, 204
Eco53kI GAGCTC 1 cut(s) 542
Eco57I CTGAAG 2 cut(s) 764, 911
Eco72I CACGTG 1 cut(s) 2014
EcoICRI GAGCTC 1 cut(s) 542
EcoO109I RGGNCCY 3 cut(s) 121, 204, 1858
EcoRI GAATTC 2 cut(s) 1223, 1654
EcoRII CCWGG 4 cut(s) 63, 81, 180, 2380
EcoT14I CCWWGG 1 cut(s) 2435
EcoT22I ATGCAT 2 cut(s) 2129, 2580
EcoT38I GRGCYC 2 cut(s) 180, 544
EgeI GGCGCC 1 cut(s) 2244
EheI GGCGCC 1 cut(s) 2244
ErhI CCWWGG 1 cut(s) 2435
Esp3I CGTCTC 2 cut(s) 29, 519
FaeI CATG 3 cut(s) 97, 1087, 2250
FaqI GGGAC 5 cut(s) 190, 665, 1004, 1008, 2511
FatI CATG 3 cut(s) 93, 1083, 2246
FauI CCCGC 6 cut(s) 180, 282, 1330, 2166, 2325, 2359
FauNDI CATATG 2 cut(s) 1417, 2407
FbaI TGATCA 2 cut(s) 1306, 2562
FblI GTMKAC 1 cut(s) 2466
Fnu4HI GCNGC 9 cut(s) 44, 165, 422, 860, 1368, 1473, 1602, 2337, 2458
FokI GGATG 5 cut(s) 442, 1073, 1369, 2038, 2346
FriOI GRGCYC 2 cut(s) 180, 544
Fsp4HI GCNGC 9 cut(s) 44, 165, 422, 860, 1368, 1473, 1602, 2337, 2458
FspBI CTAG 8 cut(s) 1353, 1650, 1697, 1713, 1862, 2063, 2144, 2183
GlaI GCGC 3 cut(s) 77, 79, 2244
GluI GCNGC 9 cut(s) 44, 165, 422, 860, 1368, 1473, 1602, 2337, 2458
GsaI CCCAGC 1 cut(s) 1160
GsuI CTGGAG 2 cut(s) 203, 2251
HaeII RGCGCY 1 cut(s) 2246
HaeIII GGCC 6 cut(s) 62, 281, 704, 875, 1859, 1946
HhaI GCGC 3 cut(s) 78, 80, 2245
Hin1I GRCGYC 1 cut(s) 2243
Hin1II CATG 3 cut(s) 97, 1087, 2250
Hin6I GCGC 3 cut(s) 76, 78, 2243
HinP1I GCGC 3 cut(s) 76, 78, 2243
HphI GGTGA 5 cut(s) 97, 271, 1324, 1352, 2081
Hpy166II GTNNAC 2 cut(s) 2429, 2467
Hpy188III TCNNGA 8 cut(s) 148, 207, 522, 993, 1047, 1084, 1172, 1481
Hpy8I GTNNAC 2 cut(s) 2429, 2467
Hpy99I CGWCG 2 cut(s) 131, 533
HpyCH4III ACNGT 6 cut(s) 315, 656, 675, 1210, 1363, 1491
HpyCH4IV ACGT 4 cut(s) 997, 2013, 2326, 2463
HpyF10VI GCNNNNNNNGC 4 cut(s) 184, 548, 1952, 2345
HpyF3I CTNAG 8 cut(s) 543, 729, 1463, 1569, 1642, 1845, 2108, 2187
HpySE526I ACGT 4 cut(s) 997, 2013, 2326, 2463
Hsp92I GRCGYC 1 cut(s) 2243
Hsp92II CATG 3 cut(s) 97, 1087, 2250
HspAI GCGC 3 cut(s) 76, 78, 2243
KasI GGCGCC 1 cut(s) 2242
KflI GGGWCCC 1 cut(s) 204
Ksp22I TGATCA 2 cut(s) 1306, 2562
Kzo9I GATC 7 cut(s) 450, 775, 1306, 1509, 1896, 2104, 2562
Lsp1109I GCAGC 6 cut(s) 55, 433, 1379, 1459, 2348, 2469
LweI GCATC 7 cut(s) 1165, 1551, 1636, 1699, 1894, 2181, 2565
MaeI CTAG 8 cut(s) 1353, 1650, 1697, 1713, 1862, 2063, 2144, 2183
MaeII ACGT 4 cut(s) 997, 2013, 2326, 2463
MaeIII GTNAC 5 cut(s) 309, 1408, 2040, 2249, 2486
MalI GATC 7 cut(s) 452, 777, 1308, 1511, 1898, 2106, 2564
MbiI CCGCTC 1 cut(s) 187
MboI GATC 7 cut(s) 450, 775, 1306, 1509, 1896, 2104, 2562
MfeI CAATTG 2 cut(s) 1142, 2521
MflI RGATCY 2 cut(s) 1509, 1896
MhlI GDGCHC 2 cut(s) 180, 544
Mly113I GGCGCC 1 cut(s) 2243
MlyI GAGTC 5 cut(s) 134, 239, 1296, 1909, 2156
MmeI TCCRAC 6 cut(s) 12, 657, 780, 1362, 1694, 1937
Mph1103I ATGCAT 2 cut(s) 2129, 2580
MroXI GAANNNNTTC 3 cut(s) 1108, 1869, 2033
MspA1I CMGCKG 2 cut(s) 46, 2332
MspR9I CCNGG 4 cut(s) 65, 83, 182, 2382
MunI CAATTG 2 cut(s) 1142, 2521
Mva1269I GAATGC 3 cut(s) 101, 167, 1603
MvaI CCWGG 4 cut(s) 65, 83, 182, 2382
MvnI CGCG 2 cut(s) 78, 215
MwoI GCNNNNNNNGC 4 cut(s) 184, 548, 1952, 2345
NarI GGCGCC 1 cut(s) 2243
NdeI CATATG 2 cut(s) 1417, 2407
NdeII GATC 7 cut(s) 450, 775, 1306, 1509, 1896, 2104, 2562
NlaIII CATG 3 cut(s) 97, 1087, 2250
NlaIV GGNNCC 5 cut(s) 179, 205, 206, 1511, 2244
NmeAIII GCCGAG 2 cut(s) 227, 1396
NmuCI GTSAC 2 cut(s) 2040, 2249
NsiI ATGCAT 2 cut(s) 2129, 2580
NspV TTCGAA 3 cut(s) 17, 339, 2474
PagI TCATGA 1 cut(s) 1083
PaqCI CACCTGC 1 cut(s) 1543
PauI GCGCGC 1 cut(s) 76
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 168
PctI GAATGC 3 cut(s) 101, 167, 1603
PdmI GAANNNNTTC 3 cut(s) 1108, 1869, 2033
PflFI GACNNNGTC 1 cut(s) 143
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 9 cut(s) 45, 166, 423, 861, 1369, 1474, 1603, 2338, 2459
PleI GAGTC 5 cut(s) 134, 239, 1295, 1909, 2155
PluTI GGCGCC 1 cut(s) 2246
PmaCI CACGTG 1 cut(s) 2014
PmlI CACGTG 1 cut(s) 2014
PpsI GAGTC 5 cut(s) 134, 239, 1295, 1909, 2155
Ppu21I YACGTR 1 cut(s) 2014
PpuMI RGGWCCY 2 cut(s) 121, 204
PsiI TTATAA 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 544
Psp5II RGGWCCY 2 cut(s) 121, 204
Psp6I CCWGG 4 cut(s) 63, 81, 180, 2380
PspCI CACGTG 1 cut(s) 2014
PspFI CCCAGC 1 cut(s) 1156
PspGI CCWGG 4 cut(s) 63, 81, 180, 2380
PspN4I GGNNCC 5 cut(s) 179, 205, 206, 1511, 2244
PspPI GGNCC 4 cut(s) 61, 121, 204, 1858
PspPPI RGGWCCY 2 cut(s) 121, 204
PsrI GAACNNNNNNTAC 2 cut(s) 143, 175
PstNI CAGNNNCTG 1 cut(s) 1118
PsuI RGATCY 2 cut(s) 1509, 1896
PsyI GACNNNGTC 1 cut(s) 143
PteI GCGCGC 1 cut(s) 76
RsaI GTAC 2 cut(s) 378, 1995
RsaNI GTAC 2 cut(s) 377, 1994
SacI GAGCTC 1 cut(s) 544
SatI GCNGC 9 cut(s) 44, 165, 422, 860, 1368, 1473, 1602, 2337, 2458
Sau3AI GATC 7 cut(s) 450, 775, 1306, 1509, 1896, 2104, 2562
Sau96I GGNCC 4 cut(s) 61, 121, 204, 1858
SchI GAGTC 5 cut(s) 134, 239, 1296, 1909, 2156
ScrFI CCNGG 4 cut(s) 65, 83, 182, 2382
SduI GDGCHC 2 cut(s) 180, 544
SfaNI GCATC 7 cut(s) 1165, 1551, 1636, 1699, 1894, 2181, 2565
SfoI GGCGCC 1 cut(s) 2244
SfuI TTCGAA 3 cut(s) 17, 339, 2474
SinI GGWCC 2 cut(s) 121, 204
SmlI CTYRAG 2 cut(s) 629, 972
SmoI CTYRAG 2 cut(s) 629, 972
SpeI ACTAGT 1 cut(s) 2062
SspDI GGCGCC 1 cut(s) 2242
SspI AATATT 1 cut(s) 1690
SspMI CTAG 8 cut(s) 1353, 1650, 1697, 1713, 1862, 2063, 2144, 2183
SstI GAGCTC 1 cut(s) 544
StyD4I CCNGG 4 cut(s) 63, 81, 180, 2380
StyI CCWWGG 1 cut(s) 2435
TaaI ACNGT 6 cut(s) 315, 656, 675, 1210, 1363, 1491
TaiI ACGT 4 cut(s) 1000, 2016, 2329, 2466
TaqII GACCGA 1 cut(s) 2155
TatI WGTACW 1 cut(s) 376
TauI GCSGC 3 cut(s) 167, 862, 1604
TscAI CASTG 3 cut(s) 645, 1593, 2397
TseFI GTSAC 2 cut(s) 2040, 2249
TseI GCWGC 6 cut(s) 43, 421, 1367, 1472, 2336, 2457
Tsp45I GTSAC 2 cut(s) 2040, 2249
TspDTI ATGAA 8 cut(s) 446, 956, 1072, 1100, 1253, 1512, 1785, 2042
TspGWI ACGGA 7 cut(s) 442, 1006, 1535, 1951, 2237, 2391, 2568
TspRI CASTG 3 cut(s) 645, 1593, 2397
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 2 cut(s) 121, 204
XapI RAATTY 7 cut(s) 239, 902, 1223, 1654, 1717, 1772, 2318
XmiI GTMKAC 1 cut(s) 2466
XmnI GAANNNNTTC 3 cut(s) 1108, 1869, 2033
XspI CTAG 8 cut(s) 1353, 1650, 1697, 1713, 1862, 2063, 2144, 2183
Zsp2I ATGCAT 2 cut(s) 2129, 2580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.