RchiOBHm_Chr3g0453081

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000126
Physical Location & Seq
Forward (+)
3611834 .. 3615705
3872 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42019

Sequence Viewer

Length: 2406 bp
ATGGAGGAGATAGCATCGGAGTTGAAGCAGAGAAATGTGAGAGAAGCGTGGAAGGAGCTGGACGACCACTTTGACTCGACCCGGAGACGGCTCCGGACCCGGTTCCAGGAGCTCCAGGCCCGCGAGGAGGAAATCGGAGTCCGTGAGAAGCTGTTGGAGGTGAAAGAGCTGAGATTAGAAGCTCAGGCCAAGGGATTGAATGAAGTTGAGAAGCGGGCCGAAGAAGGTAAGAGTCAGTTGGAGTCTCTTAGGGTTTTGATTGAAAAACATGAAGCAGAGCTTGGTGTTAAATTAAAGAGGCTCTCGGAGGTTGGGGAATTAGTTAGGGAAAAGGAGAGGGAGTATGATTTGATTCAGAATCGTGTGAAAGAGAGGGGTAAGAGACTGAACAAGTATAATCGTGCGATTGAGTTGAAAAAGGGGCAACTTGGTTCGATTCTGAAATCGATGATGGAATGCCAGAGCAGAATCGATTCGAGGGAGAAGATAATGAGGGAAATGGAGTTGAAAGGGAGGGACTTTTGTTTGCTGAAGAAATCGATGGAGGATTGGTTATGTAAAGTTGAATTGAGAAACAGGGAGCTTGATGGATGGGTTGAGGTTCTTGAGGTGAAGGAGAAGGATGTTGAGTCCAAAGTTGAGGAACTCCATTTGATTGTTGAGAGGGTCAGTGAATGCCTTGATGAGGTTCAGGTGAAAGAAGAGCATTTTGTTTCGTTAGAAAAACCGATACAGGAGCAGGAAATGCATTTGTGTTCACTCCAAAAGTTGGTGCAACAGCGTGACAAGGAGTTGGATTCACTGTCCCATGAACTTCAAAGGAAAGAGAGGCATCTTGAAGAGGTTGAATTGAAGATGGCGAAATTAGGACATGCGATTCCTTCATACGTAAGTGATGATCATTCCAGCATTCATTGGGATGGTAGAGGCTTGCAAATGTTCATGAATGAGCAGTTGAAGAGAATATATTCAATGGGTAGTGAAATGTCAGCAATTCTTAAAGCCTCATCTGACCCAGCAAAATTGGTTTTGGATGCAATGCAAGGATTTTACCCTTCAAGTTCAACTCTGGACAACAAGGAGACTCATTTTGATTTGACAGTTATTAGAAGGAGTTGTATTCTTTTGTTAGAGGATTTGAAGAGAATGTCACCACAAATTAATGTTCGGGTGAGAAACGAAGCAATGAAGTTGGCGGCTGACTGGAAGGCTAAGATAGTATCTAGTGAGAATACACAGGAGATTTTGGGGTTTCTGCGGCTTGTTAATACGTATGGATTGAGTTCGATGTATGATGCGAAAGATCTTCAAAGCCTTCTTGCTGTTGTTCATAAGCCCGAACAAGCATCTCAATCAAGTAGGGCCCTTGGTATCAAAGATAAAGCACCTGGGACCAGCATCATTTCTTCCCTTATCAAAACTGTGGAACCAGAATCTTCACTGGCTATAAATGCAAAAACTCTCTCTTCTCCAAATCCTTCTTCCCAGAATGACATGTTGGCTTCTCTTTTATTGGTCTCTGACCCAGCAGAACATGTCTTGAATAGTGTGCAGAGATCATTTTCTCAGTACTGGAAACATGAAGATGTTAGTTTCAAAGAACGTATCATGTTGAATAACATGTCTTTGTTGGAGTGTCTAACGATTATCTCACCGCATATTGGACCTCATGTGAAAGAAGATGCAAAACTTCTTGCAGCCTATTGGAAACCAAAGATGACAGGCAATACTGAAAATTCAGTTGAGACTTTAGGATTTTTGCTGTTTTTATCTACATATGGATTGGTTTCTACATTGAATACAGAGGAGATTATAAAGCTTCTTGAGGTGATATCTCAGAATAAAGGGGCTGTAGATTTATGTCAGACACATGGTTATGCAGATAAGATCTCTGATTTTATTCGGAATCTGATAGAAAGGAAGCAACTGATGGATGCTGTTGGATTTATTTGCACCTTCAACCTAGCTGACAAGCTCCCCCCAATACCACTCTTAAAACAATATGTAGAGGATGCAAAGAAGTGTTCCGAAGTAACTTGCAGCTTAAAGATACTTCATGATGAAAAGGTTAAGATTGTAGACAACCTCATAGCTGATCTAAGAGCTGGGAAACAATGCATCAAAGAATACCATCTCGAGTGCGAATACCCATATGCGGACATTGAAATGCAAATAGTTCAGCTGGAAAAACTGAAGGAGAATTGGAGATGCTCGGCGCCATCTCTTGGCTCCAGTATTGTTGAATCACAAGTGCAGAGAAAACGAAAGAAGCCTAATACTGGTAGTTCTACCCGCACGCGCATGCATTTCCATCACAACAGCAGCGTAAGCTGTGAGTACTATTTGGTGCTAACAGACCCTAGCATGATGAAGTTGCCACACAGTAACTCTTTTGGACTAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida TEX13

Protein Analysis

801

Amino Acids

92.0

Weight (kDa)

6.42

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 307 - 474 5.7e-33 Frigida-like protein
Frigida PF07899 496 - 734 7.8e-51 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1814
AccB1I GGYRCC 1 cut(s) 2215
AccB7I CCANNNNNTGG 2 cut(s) 769, 2225
AccI GTMKAC 1 cut(s) 2079
AccII CGCG 2 cut(s) 123, 2299
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 7 cut(s) 121, 214, 1196, 1258, 1655, 2156, 2293
AcsI RAATTY 1 cut(s) 1735
AcuI CTGAAG 2 cut(s) 551, 2213
AcyI GRCGYC 1 cut(s) 2216
AfaI GTAC 2 cut(s) 1571, 2339
AfiI CCNNNNNNNGG 9 cut(s) 87, 106, 127, 685, 769, 1661, 2155, 2225, 2279
AflIII ACRYGT 3 cut(s) 1494, 1534, 1620
AjnI CCWGG 3 cut(s) 105, 114, 1387
AjuI GAANNNNNNNTTGG 6 cut(s) 264, 296, 1465, 1481, 1497, 1513
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 6 cut(s) 79, 249, 376, 1076, 1522, 1739
Ama87I CYCGRG 1 cut(s) 2135
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 4 cut(s) 117, 186, 216, 1362
ApaI GGGCCC 1 cut(s) 1366
ApeKI GCWGC 3 cut(s) 1697, 2040, 2322
ApoI RAATTY 1 cut(s) 1735
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AseI ATTAAT 1 cut(s) 1161
Asp700I GAANNNNTTC 2 cut(s) 472, 967
AspLEI GCGC 2 cut(s) 2218, 2301
AspS9I GGNCC 7 cut(s) 96, 118, 216, 1362, 1363, 1392, 1664
AsuC2I CCSGG 2 cut(s) 82, 100
AsuHPI GGTGA 7 cut(s) 172, 622, 706, 1143, 1183, 1644, 1840
AvaI CYCGRG 1 cut(s) 2135
AvaII GGWCC 3 cut(s) 96, 1392, 1664
BaeGI GKGCMC 1 cut(s) 1366
BanI GGYRCC 1 cut(s) 2215
BanII GRGCYC 2 cut(s) 114, 1366
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 3 cut(s) 1709, 2052, 2334
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 3 cut(s) 107, 116, 1389
BclI TGATCA 1 cut(s) 898
BcnI CCSGG 2 cut(s) 82, 100
BcoDI GTCTC 6 cut(s) 79, 249, 376, 1076, 1522, 1739
BfaI CTAG 4 cut(s) 1224, 1964, 2361, 2404
BfmI CTRYAG 1 cut(s) 1851
BfoI RGCGCY 1 cut(s) 2219
BglII AGATCT 2 cut(s) 1303, 1887
BisI GCNGC 5 cut(s) 1197, 1259, 1698, 2041, 2323
BlsI GCNGC 5 cut(s) 1198, 1260, 1699, 2042, 2324
BmcAI AGTACT 2 cut(s) 1571, 2339
Bme1390I CCNGG 5 cut(s) 82, 100, 107, 116, 1389
Bme18I GGWCC 3 cut(s) 96, 1392, 1664
BmeT110I CYCGRG 1 cut(s) 2135
BmgT120I GGNCC 7 cut(s) 96, 118, 216, 1362, 1363, 1392, 1664
BmiI GGNNCC 8 cut(s) 92, 98, 104, 1364, 1393, 1428, 2217, 2230
BmrFI CCNGG 5 cut(s) 82, 100, 107, 116, 1389
BpmI CTGGAG 2 cut(s) 98, 2215
Bpu10I CCTNAGC 1 cut(s) 183
BpuEI CTTGAG 2 cut(s) 626, 1844
BpuMI CCSGG 2 cut(s) 82, 100
Bsa29I ATCGAT 3 cut(s) 446, 471, 539
BsaAI YACGTR 2 cut(s) 889, 1272
BsaHI GRCGYC 1 cut(s) 2216
BsaI GGTCTC 1 cut(s) 1522
BsaJI CCNNGG 3 cut(s) 189, 1366, 1388
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 536, 566
Bsc4I CCNNNNNNNGG 9 cut(s) 87, 106, 127, 685, 769, 1661, 2155, 2225, 2279
Bse1I ACTGG 5 cut(s) 1208, 1446, 1577, 2232, 2284
Bse3DI GCAATG 2 cut(s) 1044, 1191
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 3 cut(s) 107, 116, 1389
BseCI ATCGAT 3 cut(s) 446, 471, 539
BseDI CCNNGG 3 cut(s) 189, 1366, 1388
BseGI GGATG 6 cut(s) 596, 628, 925, 1039, 1939, 2017
BseLI CCNNNNNNNGG 9 cut(s) 87, 106, 127, 685, 769, 1661, 2155, 2225, 2279
BseMI GCAATG 2 cut(s) 1044, 1191
BseMII CTCAG 4 cut(s) 161, 197, 1580, 1850
BseNI ACTGG 5 cut(s) 1208, 1446, 1577, 2232, 2284
BseRI GAGGAG 3 cut(s) 20, 140, 1820
BseSI GKGCMC 1 cut(s) 1366
BseXI GCAGC 3 cut(s) 1709, 2052, 2334
BseYI CCCAGC 3 cut(s) 1015, 1525, 2105
BsgI GTGCAG 2 cut(s) 1571, 2273
Bsh1236I CGCG 2 cut(s) 123, 2299
BshFI GGCC 4 cut(s) 119, 188, 218, 1364
BshNI GGYRCC 1 cut(s) 2215
BshVI ATCGAT 3 cut(s) 446, 471, 539
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 2135
BsiSI CCGG 3 cut(s) 82, 94, 100
BslFI GGGAC 3 cut(s) 530, 790, 1405
BslI CCNNNNNNNGG 9 cut(s) 87, 106, 127, 685, 769, 1661, 2155, 2225, 2279
BsmAI GTCTC 6 cut(s) 79, 249, 376, 1076, 1522, 1739
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 3 cut(s) 530, 790, 1405
BsmI GAATGC 3 cut(s) 461, 680, 909
BsnI GGCC 4 cut(s) 119, 188, 218, 1364
Bso31I GGTCTC 1 cut(s) 1522
BsoBI CYCGRG 1 cut(s) 2135
Bsp120I GGGCCC 1 cut(s) 1362
Bsp1286I GDGCHC 2 cut(s) 114, 1366
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 5 cut(s) 898, 1303, 1556, 1887, 2095
BspACI CCGC 7 cut(s) 121, 214, 1196, 1258, 1655, 2156, 2293
BspANI GGCC 4 cut(s) 119, 188, 218, 1364
BspCNI CTCAG 4 cut(s) 162, 196, 1579, 1849
BspDI ATCGAT 3 cut(s) 446, 471, 539
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 2 cut(s) 123, 2299
BspHI TCATGA 2 cut(s) 942, 2056
BspLI GGNNCC 8 cut(s) 92, 98, 104, 1364, 1393, 1428, 2217, 2230
BspQI GCTCTTC 1 cut(s) 696
BspT107I GGYRCC 1 cut(s) 2215
BspTNI GGTCTC 1 cut(s) 1522
BsrDI GCAATG 2 cut(s) 1044, 1191
BsrI ACTGG 5 cut(s) 1208, 1446, 1577, 2232, 2284
BssECI CCNNGG 3 cut(s) 189, 1366, 1388
BssMI GATC 5 cut(s) 898, 1303, 1556, 1887, 2095
BssNI GRCGYC 1 cut(s) 2216
BssT1I CCWWGG 2 cut(s) 189, 1366
Bst2UI CCWGG 3 cut(s) 107, 116, 1389
Bst4CI ACNGT 4 cut(s) 804, 1102, 1423, 2384
Bst6I CTCTTC 5 cut(s) 696, 834, 953, 1136, 1471
BstACI GRCGYC 1 cut(s) 2216
BstAPI GCANNNNNTGC 1 cut(s) 745
BstBAI YACGTR 2 cut(s) 889, 1272
BstC8I GCNNGC 5 cut(s) 121, 216, 932, 2297, 2303
BstDEI CTNAG 8 cut(s) 170, 183, 248, 1212, 1566, 1836, 2099, 2399
BstENI CCTNNNNNAGG 1 cut(s) 683
BstF5I GGATG 6 cut(s) 596, 628, 925, 1039, 1939, 2017
BstFNI CGCG 2 cut(s) 123, 2299
BstH2I RGCGCY 1 cut(s) 2219
BstHHI GCGC 2 cut(s) 2218, 2301
BstKTI GATC 5 cut(s) 901, 1306, 1559, 1890, 2098
BstMAI GTCTC 6 cut(s) 79, 249, 376, 1076, 1522, 1739
BstMBI GATC 5 cut(s) 898, 1303, 1556, 1887, 2095
BstMWI GCNNNNNNNGC 3 cut(s) 745, 1451, 2328
BstNI CCWGG 3 cut(s) 107, 116, 1389
BstNSI RCATGY 5 cut(s) 875, 1498, 1538, 1624, 2305
BstSCI CCNGG 5 cut(s) 80, 98, 105, 114, 1387
BstSFI CTRYAG 1 cut(s) 1851
BstSLI GKGCMC 1 cut(s) 1366
BstSNI TACGTA 2 cut(s) 889, 1272
BstUI CGCG 2 cut(s) 123, 2299
BstV1I GCAGC 3 cut(s) 1709, 2052, 2334
BstX2I RGATCY 2 cut(s) 1303, 1887
BstYI RGATCY 2 cut(s) 1303, 1887
Bsu15I ATCGAT 3 cut(s) 446, 471, 539
BsuRI GGCC 4 cut(s) 119, 188, 218, 1364
BsuTUI ATCGAT 3 cut(s) 446, 471, 539
BtsCI GGATG 6 cut(s) 596, 628, 925, 1039, 1939, 2017
BtsIMutI CAGTG 3 cut(s) 676, 800, 1439
Cac8I GCNNGC 5 cut(s) 121, 216, 932, 2297, 2303
CciI TCATGA 2 cut(s) 942, 2056
CfoI GCGC 2 cut(s) 2218, 2301
Cfr13I GGNCC 7 cut(s) 96, 118, 216, 1362, 1363, 1392, 1664
ClaI ATCGAT 3 cut(s) 446, 471, 539
Csp6I GTAC 2 cut(s) 1570, 2338
CviQI GTAC 2 cut(s) 1570, 2338
DdeI CTNAG 8 cut(s) 170, 183, 248, 1212, 1566, 1836, 2099, 2399
DinI GGCGCC 1 cut(s) 2217
DpnI GATC 5 cut(s) 900, 1305, 1558, 1889, 2097
DpnII GATC 5 cut(s) 898, 1303, 1556, 1887, 2095
Eam1104I CTCTTC 5 cut(s) 696, 834, 953, 1136, 1471
EarI CTCTTC 5 cut(s) 696, 834, 953, 1136, 1471
Ecl136II GAGCTC 1 cut(s) 112
Eco105I TACGTA 2 cut(s) 889, 1272
Eco130I CCWWGG 2 cut(s) 189, 1366
Eco24I GRGCYC 2 cut(s) 114, 1366
Eco31I GGTCTC 1 cut(s) 1522
Eco32I GATATC 1 cut(s) 1833
Eco47I GGWCC 3 cut(s) 96, 1392, 1664
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 2 cut(s) 551, 2213
Eco88I CYCGRG 1 cut(s) 2135
EcoICRI GAGCTC 1 cut(s) 112
EcoNI CCTNNNNNAGG 1 cut(s) 683
EcoO109I RGGNCCY 2 cut(s) 1362, 1363
EcoRII CCWGG 3 cut(s) 105, 114, 1387
EcoRV GATATC 1 cut(s) 1833
EcoT14I CCWWGG 2 cut(s) 189, 1366
EcoT22I ATGCAT 3 cut(s) 750, 2120, 2307
EcoT38I GRGCYC 2 cut(s) 114, 1366
EgeI GGCGCC 1 cut(s) 2217
EheI GGCGCC 1 cut(s) 2217
ErhI CCWWGG 2 cut(s) 189, 1366
Esp3I CGTCTC 1 cut(s) 79
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 3 cut(s) 530, 790, 1405
FauI CCCGC 3 cut(s) 128, 207, 2300
FauNDI CATATG 2 cut(s) 1777, 2152
FbaI TGATCA 1 cut(s) 898
FblI GTMKAC 1 cut(s) 2079
Fnu4HI GCNGC 5 cut(s) 1197, 1259, 1698, 2041, 2323
FokI GGATG 6 cut(s) 603, 635, 932, 1046, 1946, 2024
FriOI GRGCYC 2 cut(s) 114, 1366
Fsp4HI GCNGC 5 cut(s) 1197, 1259, 1698, 2041, 2323
FspBI CTAG 4 cut(s) 1224, 1964, 2361, 2404
GlaI GCGC 2 cut(s) 2217, 2300
GluI GCNGC 5 cut(s) 1197, 1259, 1698, 2041, 2323
GsaI CCCAGC 3 cut(s) 1019, 1529, 2109
GsuI CTGGAG 2 cut(s) 98, 2215
HaeII RGCGCY 1 cut(s) 2219
HaeIII GGCC 4 cut(s) 119, 188, 218, 1364
HapII CCGG 3 cut(s) 82, 94, 100
HhaI GCGC 2 cut(s) 2218, 2301
Hin1I GRCGYC 1 cut(s) 2216
Hin6I GCGC 2 cut(s) 2216, 2299
HinP1I GCGC 2 cut(s) 2216, 2299
HindIII AAGCTT 1 cut(s) 1817
HpaII CCGG 3 cut(s) 82, 94, 100
HphI GGTGA 7 cut(s) 172, 622, 706, 1143, 1183, 1644, 1840
Hpy166II GTNNAC 2 cut(s) 758, 2080
Hpy188III TCNNGA 9 cut(s) 94, 605, 836, 943, 1070, 1540, 1823, 2057, 2135
Hpy8I GTNNAC 2 cut(s) 758, 2080
HpyCH4III ACNGT 4 cut(s) 804, 1102, 1423, 2384
HpyCH4IV ACGT 3 cut(s) 888, 1271, 1603
HpyF10VI GCNNNNNNNGC 3 cut(s) 745, 1451, 2328
HpyF3I CTNAG 8 cut(s) 170, 183, 248, 1212, 1566, 1836, 2099, 2399
HpySE526I ACGT 3 cut(s) 888, 1271, 1603
Hsp92I GRCGYC 1 cut(s) 2216
HspAI GCGC 2 cut(s) 2216, 2299
KasI GGCGCC 1 cut(s) 2215
Kpn2I TCCGGA 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 898
Kzo9I GATC 5 cut(s) 898, 1303, 1556, 1887, 2095
LguI GCTCTTC 1 cut(s) 696
LmnI GCTCC 8 cut(s) 55, 96, 109, 117, 580, 736, 1980, 2234
Lsp1109I GCAGC 3 cut(s) 1709, 2052, 2334
MaeI CTAG 4 cut(s) 1224, 1964, 2361, 2404
MaeII ACGT 3 cut(s) 888, 1271, 1603
MaeIII GTNAC 4 cut(s) 782, 1149, 2032, 2384
MalI GATC 5 cut(s) 900, 1305, 1558, 1889, 2097
MboI GATC 5 cut(s) 898, 1303, 1556, 1887, 2095
MflI RGATCY 2 cut(s) 1303, 1887
MhlI GDGCHC 2 cut(s) 114, 1366
Mly113I GGCGCC 1 cut(s) 2216
MlyI GAGTC 6 cut(s) 68, 147, 241, 251, 638, 1078
MmeI TCCRAC 5 cut(s) 135, 219, 774, 1611, 1921
Mph1103I ATGCAT 3 cut(s) 750, 2120, 2307
MroI TCCGGA 1 cut(s) 93
MroXI GAANNNNTTC 2 cut(s) 472, 967
MseI TTAA 8 cut(s) 288, 293, 999, 1161, 1266, 1994, 2045, 2070
MslI CAYNNNNRTG 5 cut(s) 918, 1584, 1875, 2165, 2300
MspA1I CMGCKG 1 cut(s) 2182
MspI CCGG 3 cut(s) 82, 94, 100
MspR9I CCNGG 5 cut(s) 82, 100, 107, 116, 1389
Mva1269I GAATGC 3 cut(s) 461, 680, 909
MvaI CCWGG 3 cut(s) 107, 116, 1389
MvnI CGCG 2 cut(s) 123, 2299
MwoI GCNNNNNNNGC 3 cut(s) 745, 1451, 2328
NarI GGCGCC 1 cut(s) 2216
NciI CCSGG 2 cut(s) 82, 100
NdeI CATATG 2 cut(s) 1777, 2152
NdeII GATC 5 cut(s) 898, 1303, 1556, 1887, 2095
NlaIV GGNNCC 8 cut(s) 92, 98, 104, 1364, 1393, 1428, 2217, 2230
NmeAIII GCCGAG 1 cut(s) 2192
NmuCI GTSAC 2 cut(s) 782, 1149
NsiI ATGCAT 3 cut(s) 750, 2120, 2307
NspI RCATGY 5 cut(s) 875, 1498, 1538, 1624, 2305
PaeI GCATGC 1 cut(s) 2305
PaeR7I CTCGAG 1 cut(s) 2135
PagI TCATGA 2 cut(s) 942, 2056
PciI ACATGT 3 cut(s) 1494, 1534, 1620
PciSI GCTCTTC 1 cut(s) 696
PctI GAATGC 3 cut(s) 461, 680, 909
PdmI GAANNNNTTC 2 cut(s) 472, 967
PflMI CCANNNNNTGG 2 cut(s) 769, 2225
PfoI TCCNGGA 1 cut(s) 105
PkrI GCNGC 5 cut(s) 1198, 1260, 1699, 2042, 2324
PleI GAGTC 6 cut(s) 68, 146, 240, 250, 637, 1078
PluTI GGCGCC 1 cut(s) 2219
PpsI GAGTC 6 cut(s) 68, 146, 240, 250, 637, 1078
Ppu21I YACGTR 2 cut(s) 889, 1272
PscI ACATGT 3 cut(s) 1494, 1534, 1620
PshBI ATTAAT 1 cut(s) 1161
PsiI TTATAA 1 cut(s) 1814
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 3 cut(s) 105, 114, 1387
PspFI CCCAGC 3 cut(s) 1015, 1525, 2105
PspGI CCWGG 3 cut(s) 105, 114, 1387
PspN4I GGNNCC 8 cut(s) 92, 98, 104, 1364, 1393, 1428, 2217, 2230
PspOMI GGGCCC 1 cut(s) 1362
PspPI GGNCC 7 cut(s) 96, 118, 216, 1362, 1363, 1392, 1664
PsuI RGATCY 2 cut(s) 1303, 1887
PvuII CAGCTG 1 cut(s) 2182
RsaI GTAC 2 cut(s) 1571, 2339
RsaNI GTAC 2 cut(s) 1570, 2338
RseI CAYNNNNRTG 5 cut(s) 918, 1584, 1875, 2165, 2300
SacI GAGCTC 1 cut(s) 114
SapI GCTCTTC 1 cut(s) 696
SaqAI TTAA 8 cut(s) 288, 293, 999, 1161, 1266, 1994, 2045, 2070
SatI GCNGC 5 cut(s) 1197, 1259, 1698, 2041, 2323
Sau3AI GATC 5 cut(s) 898, 1303, 1556, 1887, 2095
Sau96I GGNCC 7 cut(s) 96, 118, 216, 1362, 1363, 1392, 1664
ScaI AGTACT 2 cut(s) 1571, 2339
SchI GAGTC 6 cut(s) 68, 147, 241, 251, 638, 1078
ScrFI CCNGG 5 cut(s) 82, 100, 107, 116, 1389
SduI GDGCHC 2 cut(s) 114, 1366
SfcI CTRYAG 1 cut(s) 1851
SfoI GGCGCC 1 cut(s) 2217
Sfr274I CTCGAG 1 cut(s) 2135
SinI GGWCC 3 cut(s) 96, 1392, 1664
SlaI CTCGAG 1 cut(s) 2135
SmiMI CAYNNNNRTG 5 cut(s) 918, 1584, 1875, 2165, 2300
SmlI CTYRAG 3 cut(s) 605, 1823, 2135
SmoI CTYRAG 3 cut(s) 605, 1823, 2135
SnaBI TACGTA 2 cut(s) 889, 1272
SphI GCATGC 1 cut(s) 2305
SsiI CCGC 7 cut(s) 121, 214, 1196, 1258, 1655, 2156, 2293
SspDI GGCGCC 1 cut(s) 2215
SspMI CTAG 4 cut(s) 1224, 1964, 2361, 2404
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 5 cut(s) 80, 98, 105, 114, 1387
StyI CCWWGG 2 cut(s) 189, 1366
TaaI ACNGT 4 cut(s) 804, 1102, 1423, 2384
TaiI ACGT 3 cut(s) 891, 1274, 1606
TaqI TCGA 8 cut(s) 77, 434, 446, 471, 476, 539, 1286, 2136
TatI WGTACW 2 cut(s) 1569, 2337
TauI GCSGC 2 cut(s) 1199, 1261
Tru1I TTAA 8 cut(s) 288, 293, 999, 1161, 1266, 1994, 2045, 2070
Tru9I TTAA 8 cut(s) 288, 293, 999, 1161, 1266, 1994, 2045, 2070
TscAI CASTG 3 cut(s) 676, 807, 1446
TseFI GTSAC 2 cut(s) 782, 1149
TseI GCWGC 3 cut(s) 1697, 2040, 2322
Tsp45I GTSAC 2 cut(s) 782, 1149
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 3 cut(s) 676, 807, 1446
Van91I CCANNNNNTGG 2 cut(s) 769, 2225
VpaK11BI GGWCC 3 cut(s) 96, 1392, 1664
VspI ATTAAT 1 cut(s) 1161
XagI CCTNNNNNAGG 1 cut(s) 683
XapI RAATTY 1 cut(s) 1735
XceI RCATGY 5 cut(s) 875, 1498, 1538, 1624, 2305
XhoI CTCGAG 1 cut(s) 2135
XmiI GTMKAC 1 cut(s) 2079
XmnI GAANNNNTTC 2 cut(s) 472, 967
XspI CTAG 4 cut(s) 1224, 1964, 2361, 2404
ZrmI AGTACT 2 cut(s) 1571, 2339
Zsp2I ATGCAT 3 cut(s) 750, 2120, 2307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.