Belongs to the Frigida family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2666 |
| AatII | GACGTC | 1 cut(s) | 2804 |
| AbsI | CCTCGAGG | 1 cut(s) | 125 |
| Acc16I | TGCGCA | 1 cut(s) | 81 |
| Acc36I | ACCTGC | 1 cut(s) | 2666 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 234, 1207 |
| AccI | GTMKAC | 2 cut(s) | 2289, 3435 |
| AccII | CGCG | 2 cut(s) | 189, 279 |
| AciI | CCGC | 7 cut(s) | 187, 279, 2414, 2942, 3729, 3836, 3944 |
| AclWI | GGATC | 3 cut(s) | 2614, 3169, 3730 |
| AcoI | YGGCCR | 3 cut(s) | 609, 3726, 3837 |
| AcuI | CTGAAG | 7 cut(s) | 1314, 1382, 1419, 1629, 1734, 1839, 2780 |
| AcyI | GRCGYC | 1 cut(s) | 2801 |
| AfaI | GTAC | 3 cut(s) | 65, 662, 2742 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 58, 193, 234, 1207, 1558, 2454, 2948, 3974 |
| AflII | CTTAAG | 2 cut(s) | 821, 923 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 3756 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 590, 622, 3837, 3869 |
| Alw21I | GWGCWC | 1 cut(s) | 854 |
| Alw26I | GTCTC | 6 cut(s) | 155, 206, 2371, 2941, 3500, 3526 |
| AlwI | GGATC | 3 cut(s) | 2614, 3169, 3730 |
| Ama87I | CYCGRG | 4 cut(s) | 125, 179, 3138, 3425 |
| ApeKI | GCWGC | 5 cut(s) | 2672, 2789, 2923, 3793, 3833 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 550, 582, 3540, 3572 |
| AseI | ATTAAT | 3 cut(s) | 2034, 2115, 2379 |
| AspLEI | GCGC | 2 cut(s) | 82, 279 |
| AsuC2I | CCSGG | 2 cut(s) | 166, 209 |
| AsuHPI | GGTGA | 6 cut(s) | 2361, 2401, 2429, 2494, 2931, 3946 |
| AsuNHI | GCTAGC | 1 cut(s) | 2980 |
| AvaI | CYCGRG | 4 cut(s) | 125, 179, 3138, 3425 |
| AvaII | GGWCC | 7 cut(s) | 121, 130, 558, 1654, 2230, 2809, 2951 |
| BanII | GRGCYC | 1 cut(s) | 3673 |
| BbsI | GAAGAC | 6 cut(s) | 1134, 1239, 1344, 1449, 1554, 1941 |
| Bbv12I | GWGCWC | 1 cut(s) | 854 |
| BbvI | GCAGC | 5 cut(s) | 2684, 2776, 2935, 3805, 3820 |
| BccI | CCATC | 8 cut(s) | 397, 430, 514, 979, 1061, 2132, 2510, 3793 |
| BcnI | CCSGG | 2 cut(s) | 166, 209 |
| BcoDI | GTCTC | 6 cut(s) | 155, 206, 2371, 2941, 3500, 3526 |
| BfaI | CTAG | 8 cut(s) | 53, 158, 755, 2981, 3087, 3347, 3732, 3991 |
| BfmI | CTRYAG | 3 cut(s) | 2131, 2286, 2712 |
| BfrI | CTTAAG | 2 cut(s) | 821, 923 |
| BfuAI | ACCTGC | 1 cut(s) | 2666 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 3790 |
| BisI | GCNGC | 8 cut(s) | 280, 2673, 2790, 2924, 3729, 3794, 3834, 3837 |
| BlsI | GCNGC | 8 cut(s) | 281, 2674, 2791, 2925, 3730, 3795, 3835, 3838 |
| Bme18I | GGWCC | 7 cut(s) | 121, 130, 558, 1654, 2230, 2809, 2951 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 3756 |
| BmeT110I | CYCGRG | 4 cut(s) | 125, 179, 3138, 3425 |
| BmiI | GGNNCC | 5 cut(s) | 50, 170, 559, 2232, 2811 |
| BmrI | ACTGGG | 1 cut(s) | 2869 |
| BmsI | GCATC | 9 cut(s) | 2136, 2242, 2625, 2707, 2826, 2959, 3417, 3703, 3955 |
| BmtI | GCTAGC | 1 cut(s) | 2984 |
| BmuI | ACTGGG | 1 cut(s) | 2869 |
| BpiI | GAAGAC | 6 cut(s) | 1134, 1239, 1344, 1449, 1554, 1941 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 3106, 3138 |
| BpuEI | CTTGAG | 5 cut(s) | 1670, 1874, 1980, 2034, 2237 |
| BpuMI | CCSGG | 2 cut(s) | 166, 209 |
| Bsa29I | ATCGAT | 3 cut(s) | 297, 776, 1058 |
| BsaAI | YACGTR | 1 cut(s) | 2493 |
| BsaBI | GATNNNNATC | 2 cut(s) | 3420, 3596 |
| BsaHI | GRCGYC | 1 cut(s) | 2801 |
| BsaI | GGTCTC | 1 cut(s) | 155 |
| BsaJI | CCNNGG | 9 cut(s) | 57, 1557, 2580, 2587, 3468, 3814, 3815, 3874, 3875 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 58, 193, 234, 1207, 1558, 2454, 2948, 3974 |
| Bse118I | RCCGGY | 1 cut(s) | 456 |
| Bse1I | ACTGG | 7 cut(s) | 1500, 2073, 2426, 2864, 2989, 3317, 3916 |
| Bse3DI | GCAATG | 2 cut(s) | 2262, 3924 |
| Bse8I | GATNNNNATC | 2 cut(s) | 3420, 3596 |
| BseCI | ATCGAT | 3 cut(s) | 297, 776, 1058 |
| BseDI | CCNNGG | 9 cut(s) | 57, 1557, 2580, 2587, 3468, 3814, 3815, 3874, 3875 |
| BseJI | GATNNNNATC | 2 cut(s) | 3420, 3596 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 58, 193, 234, 1207, 1558, 2454, 2948, 3974 |
| BseMI | GCAATG | 2 cut(s) | 2262, 3924 |
| BseMII | CTCAG | 6 cut(s) | 24, 2220, 2499, 2631, 3137, 3172 |
| BseNI | ACTGG | 7 cut(s) | 1500, 2073, 2426, 2864, 2989, 3317, 3916 |
| BseRI | GAGGAG | 9 cut(s) | 61, 206, 638, 827, 953, 1502, 2344, 3554, 3957 |
| BseX3I | CGGCCG | 1 cut(s) | 3726 |
| BseXI | GCAGC | 5 cut(s) | 2684, 2776, 2935, 3805, 3820 |
| BseYI | CCCAGC | 3 cut(s) | 2233, 2812, 3959 |
| BsgI | GTGCAG | 1 cut(s) | 131 |
| Bsh1236I | CGCG | 2 cut(s) | 189, 279 |
| Bsh1285I | CGRYCG | 1 cut(s) | 3729 |
| BshVI | ATCGAT | 3 cut(s) | 297, 776, 1058 |
| BsiEI | CGRYCG | 1 cut(s) | 3729 |
| BsiHKAI | GWGCWC | 1 cut(s) | 854 |
| BsiHKCI | CYCGRG | 4 cut(s) | 125, 179, 3138, 3425 |
| BsiSI | CCGG | 3 cut(s) | 166, 208, 457 |
| BsiWI | CGTACG | 1 cut(s) | 63 |
| BslFI | GGGAC | 2 cut(s) | 571, 1113 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 58, 193, 234, 1207, 1558, 2454, 2948, 3974 |
| BsmAI | GTCTC | 6 cut(s) | 155, 206, 2371, 2941, 3500, 3526 |
| BsmBI | CGTCTC | 1 cut(s) | 206 |
| BsmFI | GGGAC | 2 cut(s) | 571, 1113 |
| BsmI | GAATGC | 3 cut(s) | 340, 791, 3750 |
| Bso31I | GGTCTC | 1 cut(s) | 155 |
| BsoBI | CYCGRG | 4 cut(s) | 125, 179, 3138, 3425 |
| Bsp1286I | GDGCHC | 2 cut(s) | 854, 3673 |
| Bsp1407I | TGTACA | 1 cut(s) | 2740 |
| Bsp19I | CCATGG | 1 cut(s) | 3468 |
| BspACI | CCGC | 7 cut(s) | 187, 279, 2414, 2942, 3729, 3836, 3944 |
| BspCNI | CTCAG | 6 cut(s) | 25, 2219, 2500, 2632, 3136, 3171 |
| BspDI | ATCGAT | 3 cut(s) | 297, 776, 1058 |
| BspFNI | CGCG | 2 cut(s) | 189, 279 |
| BspHI | TCATGA | 1 cut(s) | 2160 |
| BspLI | GGNNCC | 5 cut(s) | 50, 170, 559, 2232, 2811 |
| BspMI | ACCTGC | 1 cut(s) | 2666 |
| BspOI | GCTAGC | 1 cut(s) | 2984 |
| BspPI | GGATC | 3 cut(s) | 2614, 3169, 3730 |
| BspQI | GCTCTTC | 2 cut(s) | 744, 2799 |
| BspTI | CTTAAG | 2 cut(s) | 821, 923 |
| BspTNI | GGTCTC | 1 cut(s) | 155 |
| BsrDI | GCAATG | 2 cut(s) | 2262, 3924 |
| BsrFI | RCCGGY | 1 cut(s) | 456 |
| BsrGI | TGTACA | 1 cut(s) | 2740 |
| BsrI | ACTGG | 7 cut(s) | 1500, 2073, 2426, 2864, 2989, 3317, 3916 |
| BssAI | RCCGGY | 1 cut(s) | 456 |
| BssECI | CCNNGG | 9 cut(s) | 57, 1557, 2580, 2587, 3468, 3814, 3815, 3874, 3875 |
| BssNAI | GTATAC | 1 cut(s) | 3436 |
| BssNI | GRCGYC | 1 cut(s) | 2801 |
| BssT1I | CCWWGG | 4 cut(s) | 57, 1557, 2587, 3468 |
| Bst1107I | GTATAC | 1 cut(s) | 3436 |
| Bst4CI | ACNGT | 4 cut(s) | 109, 326, 2287, 2893 |
| Bst6I | CTCTTC | 6 cut(s) | 504, 588, 680, 744, 2171, 2799 |
| BstACI | GRCGYC | 1 cut(s) | 2801 |
| BstAFI | CTTAAG | 2 cut(s) | 821, 923 |
| BstAUI | TGTACA | 1 cut(s) | 2740 |
| BstBAI | YACGTR | 1 cut(s) | 2493 |
| BstDEI | CTNAG | 9 cut(s) | 33, 618, 1095, 2206, 2508, 2640, 3123, 3158, 3283 |
| BstDSI | CCRYGG | 1 cut(s) | 3468 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 56 |
| BstFNI | CGCG | 2 cut(s) | 189, 279 |
| BstHHI | GCGC | 2 cut(s) | 82, 279 |
| BstMAI | GTCTC | 6 cut(s) | 155, 206, 2371, 2941, 3500, 3526 |
| BstMCI | CGRYCG | 1 cut(s) | 3729 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 276, 1855, 2549, 3790 |
| BstNSI | RCATGY | 2 cut(s) | 80, 2948 |
| BstSFI | CTRYAG | 3 cut(s) | 2131, 2286, 2712 |
| BstSNI | TACGTA | 1 cut(s) | 2493 |
| BstUI | CGCG | 2 cut(s) | 189, 279 |
| BstV1I | GCAGC | 5 cut(s) | 2684, 2776, 2935, 3805, 3820 |
| BstV2I | GAAGAC | 6 cut(s) | 1134, 1239, 1344, 1449, 1554, 1941 |
| BstX2I | RGATCY | 2 cut(s) | 2606, 3174 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2588 |
| BstYI | RGATCY | 2 cut(s) | 2606, 3174 |
| BstZ17I | GTATAC | 1 cut(s) | 3436 |
| BstZI | CGGCCG | 1 cut(s) | 3726 |
| Bsu15I | ATCGAT | 3 cut(s) | 297, 776, 1058 |
| BsuTUI | ATCGAT | 3 cut(s) | 297, 776, 1058 |
| BtgI | CCRYGG | 1 cut(s) | 3468 |
| BtgZI | GCGATG | 2 cut(s) | 387, 3923 |
| BtsI | GCAGTG | 1 cut(s) | 119 |
| BveI | ACCTGC | 1 cut(s) | 2666 |
| CciI | TCATGA | 1 cut(s) | 2160 |
| CfoI | GCGC | 2 cut(s) | 82, 279 |
| Cfr10I | RCCGGY | 1 cut(s) | 456 |
| ClaI | ATCGAT | 3 cut(s) | 297, 776, 1058 |
| CsiI | ACCWGGT | 1 cut(s) | 1131 |
| Csp6I | GTAC | 3 cut(s) | 64, 661, 2741 |
| CviQI | GTAC | 3 cut(s) | 64, 661, 2741 |
| DdeI | CTNAG | 9 cut(s) | 33, 618, 1095, 2206, 2508, 2640, 3123, 3158, 3283 |
| DraI | TTTAAA | 1 cut(s) | 2358 |
| DriI | GACNNNNNGTC | 1 cut(s) | 3756 |
| EaeI | YGGCCR | 3 cut(s) | 609, 3726, 3837 |
| EagI | CGGCCG | 1 cut(s) | 3726 |
| Eam1104I | CTCTTC | 6 cut(s) | 504, 588, 680, 744, 2171, 2799 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 3756 |
| EarI | CTCTTC | 6 cut(s) | 504, 588, 680, 744, 2171, 2799 |
| EclXI | CGGCCG | 1 cut(s) | 3726 |
| Eco105I | TACGTA | 1 cut(s) | 2493 |
| Eco130I | CCWWGG | 4 cut(s) | 57, 1557, 2587, 3468 |
| Eco147I | AGGCCT | 2 cut(s) | 2093, 2222 |
| Eco24I | GRGCYC | 1 cut(s) | 3673 |
| Eco31I | GGTCTC | 1 cut(s) | 155 |
| Eco32I | GATATC | 1 cut(s) | 199 |
| Eco47I | GGWCC | 7 cut(s) | 121, 130, 558, 1654, 2230, 2809, 2951 |
| Eco52I | CGGCCG | 1 cut(s) | 3726 |
| Eco57I | CTGAAG | 7 cut(s) | 1314, 1382, 1419, 1629, 1734, 1839, 2780 |
| Eco88I | CYCGRG | 4 cut(s) | 125, 179, 3138, 3425 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 56 |
| EcoO109I | RGGNCCY | 2 cut(s) | 121, 2584 |
| EcoRI | GAATTC | 2 cut(s) | 928, 3925 |
| EcoRV | GATATC | 1 cut(s) | 199 |
| EcoT14I | CCWWGG | 4 cut(s) | 57, 1557, 2587, 3468 |
| EcoT38I | GRGCYC | 1 cut(s) | 3673 |
| ErhI | CCWWGG | 4 cut(s) | 57, 1557, 2587, 3468 |
| Esp3I | CGTCTC | 1 cut(s) | 206 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2767, 2799 |
| FaqI | GGGAC | 2 cut(s) | 571, 1113 |
| FauI | CCCGC | 3 cut(s) | 194, 2407, 3951 |
| FauNDI | CATATG | 1 cut(s) | 3064 |
| FblI | GTMKAC | 2 cut(s) | 2289, 3435 |
| Fnu4HI | GCNGC | 8 cut(s) | 280, 2673, 2790, 2924, 3729, 3794, 3834, 3837 |
| FriOI | GRGCYC | 1 cut(s) | 3673 |
| Fsp4HI | GCNGC | 8 cut(s) | 280, 2673, 2790, 2924, 3729, 3794, 3834, 3837 |
| FspBI | CTAG | 8 cut(s) | 53, 158, 755, 2981, 3087, 3347, 3732, 3991 |
| FspI | TGCGCA | 1 cut(s) | 81 |
| GlaI | GCGC | 2 cut(s) | 81, 278 |
| GluI | GCNGC | 8 cut(s) | 280, 2673, 2790, 2924, 3729, 3794, 3834, 3837 |
| GsaI | CCCAGC | 3 cut(s) | 2237, 2816, 3963 |
| HapII | CCGG | 3 cut(s) | 166, 208, 457 |
| HhaI | GCGC | 2 cut(s) | 82, 279 |
| Hin1I | GRCGYC | 1 cut(s) | 2801 |
| Hin6I | GCGC | 2 cut(s) | 80, 277 |
| HinP1I | GCGC | 2 cut(s) | 80, 277 |
| HincII | GTYRAC | 1 cut(s) | 1121 |
| HindII | GTYRAC | 1 cut(s) | 1121 |
| HpaII | CCGG | 3 cut(s) | 166, 208, 457 |
| HphI | GGTGA | 6 cut(s) | 2361, 2401, 2429, 2494, 2931, 3946 |
| Hpy166II | GTNNAC | 6 cut(s) | 144, 953, 1121, 2290, 3436, 3869 |
| Hpy8I | GTNNAC | 6 cut(s) | 144, 953, 1121, 2290, 3436, 3869 |
| HpyCH4III | ACNGT | 4 cut(s) | 109, 326, 2287, 2893 |
| HpyCH4IV | ACGT | 3 cut(s) | 663, 2492, 2801 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 276, 1855, 2549, 3790 |
| HpyF3I | CTNAG | 9 cut(s) | 33, 618, 1095, 2206, 2508, 2640, 3123, 3158, 3283 |
| HpySE526I | ACGT | 3 cut(s) | 663, 2492, 2801 |
| Hsp92I | GRCGYC | 1 cut(s) | 2801 |
| HspAI | GCGC | 2 cut(s) | 80, 277 |
| LguI | GCTCTTC | 2 cut(s) | 744, 2799 |
| LmnI | GCTCC | 5 cut(s) | 48, 1561, 2524, 2920, 3008 |
| Lsp1109I | GCAGC | 5 cut(s) | 2684, 2776, 2935, 3805, 3820 |
| LweI | GCATC | 9 cut(s) | 2136, 2242, 2625, 2707, 2826, 2959, 3417, 3703, 3955 |
| MabI | ACCWGGT | 1 cut(s) | 1131 |
| MaeI | CTAG | 8 cut(s) | 53, 158, 755, 2981, 3087, 3347, 3732, 3991 |
| MaeII | ACGT | 3 cut(s) | 663, 2492, 2801 |
| MaeIII | GTNAC | 4 cut(s) | 316, 2417, 2485, 2903 |
| MfeI | CAATTG | 3 cut(s) | 692, 714, 2547 |
| MflI | RGATCY | 2 cut(s) | 2606, 3174 |
| MhlI | GDGCHC | 2 cut(s) | 854, 3673 |
| MlyI | GAGTC | 7 cut(s) | 23, 38, 139, 213, 623, 3130, 3437 |
| MmeI | TCCRAC | 6 cut(s) | 201, 2109, 2439, 2808, 2929, 3733 |
| MslI | CAYNNNNRTG | 2 cut(s) | 372, 3777 |
| MspA1I | CMGCKG | 1 cut(s) | 2210 |
| MspCI | CTTAAG | 2 cut(s) | 821, 923 |
| MspI | CCGG | 3 cut(s) | 166, 208, 457 |
| MunI | CAATTG | 3 cut(s) | 692, 714, 2547 |
| Mva1269I | GAATGC | 3 cut(s) | 340, 791, 3750 |
| MvnI | CGCG | 2 cut(s) | 189, 279 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 276, 1855, 2549, 3790 |
| NciI | CCSGG | 2 cut(s) | 166, 209 |
| NcoI | CCATGG | 1 cut(s) | 3468 |
| NdeI | CATATG | 1 cut(s) | 3064 |
| NheI | GCTAGC | 1 cut(s) | 2980 |
| NlaIV | GGNNCC | 5 cut(s) | 50, 170, 559, 2232, 2811 |
| NmeAIII | GCCGAG | 2 cut(s) | 637, 2578 |
| NmuCI | GTSAC | 1 cut(s) | 2417 |
| NsbI | TGCGCA | 1 cut(s) | 81 |
| NspI | RCATGY | 2 cut(s) | 80, 2948 |
| PaeR7I | CTCGAG | 4 cut(s) | 125, 179, 3138, 3425 |
| PagI | TCATGA | 1 cut(s) | 2160 |
| PaqCI | CACCTGC | 1 cut(s) | 2666 |
| PasI | CCCWGGG | 2 cut(s) | 3815, 3875 |
| PceI | AGGCCT | 2 cut(s) | 2093, 2222 |
| PciSI | GCTCTTC | 2 cut(s) | 744, 2799 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 186, 773 |
| PctI | GAATGC | 3 cut(s) | 340, 791, 3750 |
| Pfl23II | CGTACG | 1 cut(s) | 63 |
| PflFI | GACNNNGTC | 1 cut(s) | 1133 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 234, 1207 |
| PfoI | TCCNGGA | 1 cut(s) | 207 |
| PkrI | GCNGC | 8 cut(s) | 281, 2674, 2791, 2925, 3730, 3795, 3835, 3838 |
| PleI | GAGTC | 7 cut(s) | 22, 37, 139, 212, 622, 3130, 3436 |
| PpsI | GAGTC | 7 cut(s) | 22, 37, 139, 212, 622, 3130, 3436 |
| Ppu21I | YACGTR | 1 cut(s) | 2493 |
| PpuMI | RGGWCCY | 1 cut(s) | 121 |
| PshBI | ATTAAT | 3 cut(s) | 2034, 2115, 2379 |
| Psp5II | RGGWCCY | 1 cut(s) | 121 |
| PspFI | CCCAGC | 3 cut(s) | 2233, 2812, 3959 |
| PspLI | CGTACG | 1 cut(s) | 63 |
| PspN4I | GGNNCC | 5 cut(s) | 50, 170, 559, 2232, 2811 |
| PspPPI | RGGWCCY | 1 cut(s) | 121 |
| PspXI | VCTCGAGB | 2 cut(s) | 125, 179 |
| PsuI | RGATCY | 2 cut(s) | 2606, 3174 |
| PsyI | GACNNNGTC | 1 cut(s) | 1133 |
| PvuII | CAGCTG | 1 cut(s) | 2210 |
| RsaI | GTAC | 3 cut(s) | 65, 662, 2742 |
| RsaNI | GTAC | 3 cut(s) | 64, 661, 2741 |
| RseI | CAYNNNNRTG | 2 cut(s) | 372, 3777 |
| SapI | GCTCTTC | 2 cut(s) | 744, 2799 |
| SatI | GCNGC | 8 cut(s) | 280, 2673, 2790, 2924, 3729, 3794, 3834, 3837 |
| SchI | GAGTC | 7 cut(s) | 23, 38, 139, 213, 623, 3130, 3437 |
| SduI | GDGCHC | 2 cut(s) | 854, 3673 |
| SexAI | ACCWGGT | 1 cut(s) | 1131 |
| SfaNI | GCATC | 9 cut(s) | 2136, 2242, 2625, 2707, 2826, 2959, 3417, 3703, 3955 |
| SfcI | CTRYAG | 3 cut(s) | 2131, 2286, 2712 |
| Sfr274I | CTCGAG | 4 cut(s) | 125, 179, 3138, 3425 |
| SinI | GGWCC | 7 cut(s) | 121, 130, 558, 1654, 2230, 2809, 2951 |
| SlaI | CTCGAG | 4 cut(s) | 125, 179, 3138, 3425 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 372, 3777 |
| SnaBI | TACGTA | 1 cut(s) | 2493 |
| SseBI | AGGCCT | 2 cut(s) | 2093, 2222 |
| SsiI | CCGC | 7 cut(s) | 187, 279, 2414, 2942, 3729, 3836, 3944 |
| SspI | AATATT | 1 cut(s) | 883 |
| SspMI | CTAG | 8 cut(s) | 53, 158, 755, 2981, 3087, 3347, 3732, 3991 |
| StuI | AGGCCT | 2 cut(s) | 2093, 2222 |
| StyI | CCWWGG | 4 cut(s) | 57, 1557, 2587, 3468 |
| TaaI | ACNGT | 4 cut(s) | 109, 326, 2287, 2893 |
| TaiI | ACGT | 3 cut(s) | 666, 2495, 2804 |
| TaqII | GACCGA | 1 cut(s) | 575 |
| TatI | WGTACW | 1 cut(s) | 2740 |
| TauI | GCSGC | 3 cut(s) | 282, 3731, 3839 |
| TseFI | GTSAC | 1 cut(s) | 2417 |
| TseI | GCWGC | 5 cut(s) | 2672, 2789, 2923, 3793, 3833 |
| Tsp45I | GTSAC | 1 cut(s) | 2417 |
| TspGWI | ACGGA | 1 cut(s) | 1425 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1133 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 234, 1207 |
| Vha464I | CTTAAG | 2 cut(s) | 821, 923 |
| VpaK11BI | GGWCC | 7 cut(s) | 121, 130, 558, 1654, 2230, 2809, 2951 |
| VspI | ATTAAT | 3 cut(s) | 2034, 2115, 2379 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 56 |
| XceI | RCATGY | 2 cut(s) | 80, 2948 |
| XhoI | CTCGAG | 4 cut(s) | 125, 179, 3138, 3425 |
| XmiI | GTMKAC | 2 cut(s) | 2289, 3435 |
| XspI | CTAG | 8 cut(s) | 53, 158, 755, 2981, 3087, 3347, 3732, 3991 |
| ZraI | GACGTC | 1 cut(s) | 2802 |