RchiOBHm_Chr3g0453041

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000126
Physical Location & Seq
Forward (+)
3569865 .. 3573762
3898 bp
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UTR
Exon/CDS
Intron
PRQ42015

Sequence Viewer

Length: 2370 bp
ATGGAAGAGATGATCTCGGATTTGAAAGAATCGGAGGTAGACCAAATTGCTCTGGCCGAAGCATTCGAGAGTTTGCTAGCCCGCTGCAAGCAAATTGGGGACCAGTTCATCTTGACCCGGAGCACAATCTCGACCCGGTTTAAGGAGCTCCAGACCCGCGAGGAGGAAATTGGGCGCCGGGAGAAGCTTCTGGAAGAGAAAGAGTCCGGCTTTAAGTCGAAGGAGGAGGAGTTGGATGCAATTGAGGAGGGCATTACAGAGAAGCTACTGGAGTTTGAAGAAGGTAAGACCCAATTGGAAAGACTTAATTTTTCGATTGATGATAAAGAGAGGAGGGTTTTGGAAGTTGAGGAATTGATTAGGGTAAAAGAGAGGGAGTGTGATTTGATCGAAAATCGTATCGAAAAGGGTACCAATATGATGATTAGGATTGAGGAGAGTATTAAAGAAATGGATTTGAAGGTGAAAGGTTTTGACTTGCATAAGAAAGAAATGGAGGATTGGAGCAGTAAGCTTGAATTGAAAGAGAAGGAACTAGAGAGGTTGTTTGAGAAGCTTAGATTGAAAGAGGCGTTATTAGAACCAAATGTTGTAGAGCTTCATGTTCTTGCTAACAGCGTCGAAGAGTGCCTCAATGAAGCTCAAAGGAGGGAGAGGGAAGTTGAATTGAAAGGGAGACGAGTTGAATCAAGGGTTAAGGAAGTAGAGTTGGCTGAGATGAGGGTCGAAGAGTGCCTCAATGAAGCTCAAAGGAGGGAGAGGGAAGTTGAATTGAAAGAGAGACGAGTTGAATCAAGAGTTAAGGAAGTTGAGTTGGCTGAGATGAGGGTCAATGAGTGCCTCAATGAAGGTCAGAGGAAAGAGAGGGAAGTTGAATTGAAAGAGAGACGAGTTGAATCAAGAGTTAAGGAAGTTGAGTTGGCTGAGATGAGGGTCAATGAGTGCCTCAATGAAGCTCACAGGAAAGAGAAGGAAGTTGAATCGAAAGTTAAGGAACTTGAGTTGACTGAGATGAGGGTCAAGGAACGCCTCAATAAGGTTGAATTGAAAGAAGAGCATCTTTGTTTAATGGAGAAGTTGATGGAAGAAAACAAAAGACGTTTGGATGTACTCATGAAGACTTCAGAAGAGTTGGAAAAACACTTGGATTCACTCCAAAAGTCGGTGGAAGAGCGCGAAAGGAATTTGGATTTGGTATCTGATGAACTTAAAGGGAAAGAGAGGGAATTTCAACAGCGGGCCGAAGCGCTTGAATTGAACTGGGCGCAATTTGATTCGCCAGGGAAGATTCAGCAGTTGGAATATACACCTGGGGTTGACAATGCGGTTGTTTCTTCATCTGTGACCAACAACATTTCTTCCTTTGTCAAAGCGGACGAACCAGAATCTTCACTGTCTAAAAATGCAGCAAGTTTTCTTTCCTCAAATCTTCAACCAAATGCCACCAGTGATGAAGGATATGTCCCAGGGTTTCTAAATGAGAATTTAAGTGGGAATAATTTCATAGATAATGAATTGGCTGCTTCTCTTCGGTTGTTACAGGACCCAGCTAAACTCGTGTTATATAATATGCAAAAATCCCTTGATCAGTACTTAAGAAATGGATATTTTGAAGAAAGTATAATGTCAGGTAACCTTTCCATATTGAAGGAGCTAATGAGTATTTCACCACATGTTGGACCTCATCTGAAAGTAGATGCAACATATCTAGCATCCCAGTGGAAAACAAAGATGAGAGGCAATACCAAAAATTCAGTGGAGAGTTTGGCATTCTTGTTGTTTATAACTCTGTATGGATTGGTTTCTATGTTGAATGTAGATGAGATAGTAAAGCTTCTTGGGTTGATTTCTCAGAATAAAAGGGCTCTCGAATTATGTCAGACACATGCTTTTGCAGTTAAGATCGCTGATCTTATTCACAGTCTTATTGAAAGGAAGCAGCTGCCTGTGGCTGTTAGATGTATTTGCATGCTCAAGTTCACTGACATGCTCCCCCTAGTACCACTCCTAGAAGAGTATGTGCAGGATGCAATGGAAAGTTTCAAAGAAATTTGTGGGTTAAGGATTACTCCTGATGAAAAGGTCAAGATTTTTAAAAAACAAATAGCTGATGCAAGAGCTTTGGTTCAATGCCTCAAAGATTACAACCTTGGATCCGAACACCCGTCCAATGCCATTGAAATACAAATAGTTCAGCTGGAAACACTGGCGGAGAATTGGAAACTTTTGGTGCCCCCTCTTTGTCCCAGTATCATTGATCAACACAAGGATGGAAAAAGAAAGAAGCATAATACTAGCACTTCTGACCCTGAGTTCCAACCACAACAGCAGCGTAAAATGAAGATTGTCTTTCGTGGTATTGGACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida

Protein Analysis

789

Amino Acids

91.6

Weight (kDa)

5.21

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 491 - 737 5.9e-39 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1784
Acc65I GGTACC 1 cut(s) 410
AccB1I GGYRCC 3 cut(s) 174, 410, 2230
AccB7I CCANNNNNTGG 1 cut(s) 1676
AccI GTMKAC 1 cut(s) 39
AccII CGCG 2 cut(s) 159, 1176
AciI CCGC 6 cut(s) 82, 157, 1237, 1325, 1373, 2210
AclWI GGATC 2 cut(s) 2148, 2161
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 5 cut(s) 1183, 1226, 1483, 1750, 2049
AcuI CTGAAG 1 cut(s) 1107
AcyI GRCGYC 1 cut(s) 175
AfaI GTAC 4 cut(s) 412, 1110, 1592, 2001
AfeI AGCGCT 1 cut(s) 1248
AfiI CCNNNNNNNGG 5 cut(s) 142, 163, 1036, 1162, 1676
AflII CTTAAG 1 cut(s) 1594
AflIII ACRYGT 1 cut(s) 1672
AjnI CCWGG 3 cut(s) 1279, 1309, 1465
AjuI GAANNNNNNNTTGG 2 cut(s) 1175, 1207
Alw21I GWGCWC 2 cut(s) 125, 150
Alw26I GTCTC 3 cut(s) 670, 775, 880
AlwI GGATC 2 cut(s) 2148, 2161
Aor51HI AGCGCT 1 cut(s) 1248
AoxI GGCC 2 cut(s) 54, 1239
ApeKI GCWGC 6 cut(s) 84, 1406, 1520, 1939, 1942, 2329
ApoI RAATTY 5 cut(s) 1183, 1226, 1483, 1750, 2049
Asp700I GAANNNNTTC 1 cut(s) 1499
Asp718I GGTACC 1 cut(s) 410
AspLEI GCGC 4 cut(s) 177, 1176, 1249, 1267
AspS9I GGNCC 5 cut(s) 100, 1239, 1543, 1679, 2363
AsuC2I CCSGG 3 cut(s) 118, 136, 179
AsuHPI GGTGA 2 cut(s) 475, 1659
AsuNHI GCTAGC 1 cut(s) 76
AvaII GGWCC 4 cut(s) 100, 1543, 1679, 2363
BaeGI GKGCMC 1 cut(s) 2235
BamHI GGATCC 1 cut(s) 2153
BanI GGYRCC 3 cut(s) 174, 410, 2230
BanII GRGCYC 2 cut(s) 150, 1867
BauI CACGAG 1 cut(s) 1556
BbsI GAAGAC 1 cut(s) 1124
Bbv12I GWGCWC 2 cut(s) 125, 150
BbvI GCAGC 6 cut(s) 71, 1418, 1507, 1929, 1951, 2341
BccI CCATC 2 cut(s) 1075, 2264
BciT130I CCWGG 3 cut(s) 1281, 1311, 1467
BclI TGATCA 2 cut(s) 1585, 2257
BcnI CCSGG 3 cut(s) 118, 136, 179
BcoDI GTCTC 3 cut(s) 670, 775, 880
BfaI CTAG 7 cut(s) 77, 536, 1709, 1997, 2009, 2295, 2368
BfoI RGCGCY 2 cut(s) 178, 1250
BfrI CTTAAG 1 cut(s) 1594
BisI GCNGC 6 cut(s) 85, 1407, 1521, 1940, 1943, 2330
BlsI GCNGC 6 cut(s) 86, 1408, 1522, 1941, 1944, 2331
BmcAI AGTACT 1 cut(s) 1592
Bme1390I CCNGG 6 cut(s) 118, 136, 179, 1281, 1311, 1467
Bme18I GGWCC 4 cut(s) 100, 1543, 1679, 2363
BmgT120I GGNCC 5 cut(s) 100, 1239, 1543, 1679, 2363
BmiI GGNNCC 7 cut(s) 101, 176, 412, 1545, 2155, 2232, 2365
BmrFI CCNGG 6 cut(s) 118, 136, 179, 1281, 1311, 1467
BmrI ACTGGG 3 cut(s) 1270, 1711, 2241
BmsI GCATC 6 cut(s) 226, 1066, 1687, 1721, 2017, 2101
BmtI GCTAGC 1 cut(s) 80
BmuI ACTGGG 3 cut(s) 1270, 1711, 2241
BpiI GAAGAC 1 cut(s) 1124
BplI GAGNNNNNCTC 1 cut(s) 31
BpmI CTGGAG 2 cut(s) 134, 290
BpuEI CTTGAG 2 cut(s) 1019, 1958
BpuMI CCSGG 3 cut(s) 118, 136, 179
BsaHI GRCGYC 1 cut(s) 175
BsaJI CCNNGG 5 cut(s) 1280, 1310, 1465, 1466, 2149
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 5 cut(s) 142, 163, 1036, 1162, 1676
Bse1I ACTGG 7 cut(s) 103, 273, 1265, 1446, 1717, 2211, 2247
Bse3DI GCAATG 1 cut(s) 2037
BseBI CCWGG 3 cut(s) 1281, 1311, 1467
BseDI CCNNGG 5 cut(s) 1280, 1310, 1465, 1466, 2149
BseGI GGATG 5 cut(s) 241, 1111, 1712, 2032, 2275
BseLI CCNNNNNNNGG 5 cut(s) 142, 163, 1036, 1162, 1676
BseMI GCAATG 1 cut(s) 2037
BseMII CTCAG 6 cut(s) 705, 810, 915, 999, 1865, 2301
BseNI ACTGG 7 cut(s) 103, 273, 1265, 1446, 1717, 2211, 2247
BseRI GAGGAG 6 cut(s) 176, 239, 242, 260, 346, 449
BseSI GKGCMC 1 cut(s) 2235
BseXI GCAGC 6 cut(s) 71, 1418, 1507, 1929, 1951, 2341
BseYI CCCAGC 1 cut(s) 1546
BsgI GTGCAG 1 cut(s) 2042
Bsh1236I CGCG 2 cut(s) 159, 1176
BshFI GGCC 2 cut(s) 56, 1241
BshNI GGYRCC 3 cut(s) 174, 410, 2230
BsiHKAI GWGCWC 2 cut(s) 125, 150
BsiSI CCGG 4 cut(s) 118, 136, 178, 207
BslFI GGGAC 3 cut(s) 113, 1448, 2229
BslI CCNNNNNNNGG 5 cut(s) 142, 163, 1036, 1162, 1676
BsmAI GTCTC 3 cut(s) 670, 775, 880
BsmBI CGTCTC 3 cut(s) 670, 775, 880
BsmFI GGGAC 3 cut(s) 113, 1448, 2229
BsmI GAATGC 2 cut(s) 62, 1769
BsnI GGCC 2 cut(s) 56, 1241
Bsp1286I GDGCHC 4 cut(s) 125, 150, 1867, 2235
Bsp143I GATC 7 cut(s) 12, 387, 1585, 1902, 1909, 2153, 2257
BspACI CCGC 6 cut(s) 82, 157, 1237, 1325, 1373, 2210
BspANI GGCC 2 cut(s) 56, 1241
BspCNI CTCAG 6 cut(s) 706, 811, 916, 1000, 1864, 2302
BspFNI CGCG 2 cut(s) 159, 1176
BspHI TCATGA 1 cut(s) 1113
BspLI GGNNCC 7 cut(s) 101, 176, 412, 1545, 2155, 2232, 2365
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 2 cut(s) 2148, 2161
BspQI GCTCTTC 2 cut(s) 1047, 1164
BspT107I GGYRCC 3 cut(s) 174, 410, 2230
BspTI CTTAAG 1 cut(s) 1594
BsrDI GCAATG 1 cut(s) 2037
BsrI ACTGG 7 cut(s) 103, 273, 1265, 1446, 1717, 2211, 2247
BssECI CCNNGG 5 cut(s) 1280, 1310, 1465, 1466, 2149
BssMI GATC 7 cut(s) 12, 387, 1585, 1902, 1909, 2153, 2257
BssNI GRCGYC 1 cut(s) 175
BssSI CACGAG 1 cut(s) 1556
BssT1I CCWWGG 1 cut(s) 2149
Bst2BI CACGAG 1 cut(s) 1556
Bst2UI CCWGG 3 cut(s) 1281, 1311, 1467
Bst4CI ACNGT 2 cut(s) 1395, 1922
Bst6I CTCTTC 8 cut(s) 189, 618, 723, 1047, 1122, 1164, 1533, 2007
BstACI GRCGYC 1 cut(s) 175
BstAFI CTTAAG 1 cut(s) 1594
BstC8I GCNNGC 5 cut(s) 78, 82, 89, 1239, 1970
BstDEI CTNAG 7 cut(s) 557, 714, 819, 924, 1008, 1851, 2310
BstEII GGTNACC 1 cut(s) 1631
BstENI CCTNNNNNAGG 1 cut(s) 1034
BstF5I GGATG 5 cut(s) 241, 1111, 1712, 2032, 2275
BstFNI CGCG 2 cut(s) 159, 1176
BstH2I RGCGCY 2 cut(s) 178, 1250
BstHHI GCGC 4 cut(s) 177, 1176, 1249, 1267
BstKTI GATC 7 cut(s) 15, 390, 1588, 1905, 1912, 2156, 2260
BstMAI GTCTC 3 cut(s) 670, 775, 880
BstMBI GATC 7 cut(s) 12, 387, 1585, 1902, 1909, 2153, 2257
BstNI CCWGG 3 cut(s) 1281, 1311, 1467
BstNSI RCATGY 4 cut(s) 1676, 1889, 1972, 1990
BstPI GGTNACC 1 cut(s) 1631
BstSCI CCNGG 6 cut(s) 116, 134, 177, 1279, 1309, 1465
BstSLI GKGCMC 1 cut(s) 2235
BstUI CGCG 2 cut(s) 159, 1176
BstV1I GCAGC 6 cut(s) 71, 1418, 1507, 1929, 1951, 2341
BstV2I GAAGAC 1 cut(s) 1124
BstX2I RGATCY 1 cut(s) 2153
BstYI RGATCY 1 cut(s) 2153
BsuRI GGCC 2 cut(s) 56, 1241
BtsCI GGATG 5 cut(s) 241, 1111, 1712, 2032, 2275
BtsIMutI CAGTG 6 cut(s) 1391, 1453, 1724, 1761, 1980, 2204
Cac8I GCNNGC 5 cut(s) 78, 82, 89, 1239, 1970
CciI TCATGA 1 cut(s) 1113
CfoI GCGC 4 cut(s) 177, 1176, 1249, 1267
Cfr13I GGNCC 5 cut(s) 100, 1239, 1543, 1679, 2363
CseI GACGC 1 cut(s) 607
Csp6I GTAC 4 cut(s) 411, 1109, 1591, 2000
CviAII CATG 6 cut(s) 602, 1114, 1673, 1886, 1969, 1987
CviQI GTAC 4 cut(s) 411, 1109, 1591, 2000
DdeI CTNAG 7 cut(s) 557, 714, 819, 924, 1008, 1851, 2310
DinI GGCGCC 1 cut(s) 176
DpnI GATC 7 cut(s) 14, 389, 1587, 1904, 1911, 2155, 2259
DpnII GATC 7 cut(s) 12, 387, 1585, 1902, 1909, 2153, 2257
DraI TTTAAA 1 cut(s) 2095
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 8 cut(s) 189, 618, 723, 1047, 1122, 1164, 1533, 2007
EarI CTCTTC 8 cut(s) 189, 618, 723, 1047, 1122, 1164, 1533, 2007
EciI GGCGGA 1 cut(s) 2225
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 2149
Eco24I GRGCYC 2 cut(s) 150, 1867
Eco47I GGWCC 4 cut(s) 100, 1543, 1679, 2363
Eco47III AGCGCT 1 cut(s) 1248
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 1107
Eco91I GGTNACC 1 cut(s) 1631
EcoICRI GAGCTC 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 1034
EcoO109I RGGNCCY 1 cut(s) 1543
EcoO65I GGTNACC 1 cut(s) 1631
EcoRII CCWGG 3 cut(s) 1279, 1309, 1465
EcoT14I CCWWGG 1 cut(s) 2149
EcoT38I GRGCYC 2 cut(s) 150, 1867
EgeI GGCGCC 1 cut(s) 176
EheI GGCGCC 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 2149
Esp3I CGTCTC 3 cut(s) 670, 775, 880
FaeI CATG 6 cut(s) 605, 1117, 1676, 1889, 1972, 1990
FalI AAGNNNNNCTT 4 cut(s) 1044, 1076, 2333, 2365
FaqI GGGAC 3 cut(s) 113, 1448, 2229
FatI CATG 6 cut(s) 601, 1113, 1672, 1885, 1968, 1986
FauI CCCGC 3 cut(s) 89, 164, 1230
FbaI TGATCA 2 cut(s) 1585, 2257
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 6 cut(s) 85, 1407, 1521, 1940, 1943, 2330
FokI GGATG 5 cut(s) 248, 1118, 1699, 2039, 2282
FriOI GRGCYC 2 cut(s) 150, 1867
Fsp4HI GCNGC 6 cut(s) 85, 1407, 1521, 1940, 1943, 2330
FspBI CTAG 7 cut(s) 77, 536, 1709, 1997, 2009, 2295, 2368
GlaI GCGC 4 cut(s) 176, 1175, 1248, 1266
GluI GCNGC 6 cut(s) 85, 1407, 1521, 1940, 1943, 2330
GsaI CCCAGC 1 cut(s) 1550
GsuI CTGGAG 2 cut(s) 134, 290
HaeII RGCGCY 2 cut(s) 178, 1250
HaeIII GGCC 2 cut(s) 56, 1241
HapII CCGG 4 cut(s) 118, 136, 178, 207
HgaI GACGC 1 cut(s) 607
HhaI GCGC 4 cut(s) 177, 1176, 1249, 1267
Hin1I GRCGYC 1 cut(s) 175
Hin1II CATG 6 cut(s) 605, 1117, 1676, 1889, 1972, 1990
Hin6I GCGC 4 cut(s) 175, 1174, 1247, 1265
HinP1I GCGC 4 cut(s) 175, 1174, 1247, 1265
HincII GTYRAC 2 cut(s) 1005, 1318
HindII GTYRAC 2 cut(s) 1005, 1318
HindIII AAGCTT 4 cut(s) 185, 512, 554, 1832
HpaII CCGG 4 cut(s) 118, 136, 178, 207
HphI GGTGA 2 cut(s) 475, 1659
Hpy166II GTNNAC 4 cut(s) 40, 1005, 1318, 1980
Hpy8I GTNNAC 4 cut(s) 40, 1005, 1318, 1980
Hpy99I CGWCG 1 cut(s) 623
HpyAV CCTTC 8 cut(s) 214, 275, 454, 523, 842, 964, 1448, 1642
HpyCH4III ACNGT 2 cut(s) 1395, 1922
HpyCH4IV ACGT 1 cut(s) 1099
HpyF3I CTNAG 7 cut(s) 557, 714, 819, 924, 1008, 1851, 2310
HpySE526I ACGT 1 cut(s) 1099
Hsp92I GRCGYC 1 cut(s) 175
Hsp92II CATG 6 cut(s) 605, 1117, 1676, 1889, 1972, 1990
HspAI GCGC 4 cut(s) 175, 1174, 1247, 1265
KasI GGCGCC 1 cut(s) 174
KpnI GGTACC 1 cut(s) 414
Ksp22I TGATCA 2 cut(s) 1585, 2257
Kzo9I GATC 7 cut(s) 12, 387, 1585, 1902, 1909, 2153, 2257
LguI GCTCTTC 2 cut(s) 1047, 1164
LmnI GCTCC 6 cut(s) 120, 145, 153, 504, 1651, 1995
Lsp1109I GCAGC 6 cut(s) 71, 1418, 1507, 1929, 1951, 2341
LweI GCATC 6 cut(s) 226, 1066, 1687, 1721, 2017, 2101
MaeI CTAG 7 cut(s) 77, 536, 1709, 1997, 2009, 2295, 2368
MaeII ACGT 1 cut(s) 1099
MaeIII GTNAC 3 cut(s) 1342, 1536, 1631
MalI GATC 7 cut(s) 14, 389, 1587, 1904, 1911, 2155, 2259
MboI GATC 7 cut(s) 12, 387, 1585, 1902, 1909, 2153, 2257
MfeI CAATTG 2 cut(s) 240, 293
MflI RGATCY 1 cut(s) 2153
MhlI GDGCHC 4 cut(s) 125, 150, 1867, 2235
Mly113I GGCGCC 1 cut(s) 175
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 5 cut(s) 213, 1113, 1278, 1657, 2341
MroXI GAANNNNTTC 1 cut(s) 1499
MslI CAYNNNNRTG 3 cut(s) 1717, 1985, 2268
MspA1I CMGCKG 4 cut(s) 84, 1237, 1942, 2197
MspCI CTTAAG 1 cut(s) 1594
MspI CCGG 4 cut(s) 118, 136, 178, 207
MspR9I CCNGG 6 cut(s) 118, 136, 179, 1281, 1311, 1467
MunI CAATTG 2 cut(s) 240, 293
Mva1269I GAATGC 2 cut(s) 62, 1769
MvaI CCWGG 3 cut(s) 1281, 1311, 1467
MvnI CGCG 2 cut(s) 159, 1176
NarI GGCGCC 1 cut(s) 175
NciI CCSGG 3 cut(s) 118, 136, 179
NdeII GATC 7 cut(s) 12, 387, 1585, 1902, 1909, 2153, 2257
NheI GCTAGC 1 cut(s) 76
NlaIII CATG 6 cut(s) 605, 1117, 1676, 1889, 1972, 1990
NlaIV GGNNCC 7 cut(s) 101, 176, 412, 1545, 2155, 2232, 2365
NmuCI GTSAC 1 cut(s) 1342
NspI RCATGY 4 cut(s) 1676, 1889, 1972, 1990
PaeI GCATGC 1 cut(s) 1972
PagI TCATGA 1 cut(s) 1113
PasI CCCWGGG 1 cut(s) 1466
PciI ACATGT 1 cut(s) 1672
PciSI GCTCTTC 2 cut(s) 1047, 1164
PctI GAATGC 2 cut(s) 62, 1769
PdmI GAANNNNTTC 1 cut(s) 1499
PfeI GAWTC 9 cut(s) 29, 686, 791, 896, 980, 1148, 1274, 1288, 1385
PflMI CCANNNNNTGG 1 cut(s) 1676
PkrI GCNGC 6 cut(s) 86, 1408, 1522, 1941, 1944, 2331
PleI GAGTC 1 cut(s) 211
PluTI GGCGCC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 211
PpuMI RGGWCCY 1 cut(s) 1543
PscI ACATGT 1 cut(s) 1672
PsiI TTATAA 1 cut(s) 1784
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 1543
Psp6I CCWGG 3 cut(s) 1279, 1309, 1465
PspEI GGTNACC 1 cut(s) 1631
PspFI CCCAGC 1 cut(s) 1546
PspGI CCWGG 3 cut(s) 1279, 1309, 1465
PspN4I GGNNCC 7 cut(s) 101, 176, 412, 1545, 2155, 2232, 2365
PspPI GGNCC 5 cut(s) 100, 1239, 1543, 1679, 2363
PspPPI RGGWCCY 1 cut(s) 1543
PsuI RGATCY 1 cut(s) 2153
PvuII CAGCTG 2 cut(s) 1942, 2197
RsaI GTAC 4 cut(s) 412, 1110, 1592, 2001
RsaNI GTAC 4 cut(s) 411, 1109, 1591, 2000
RseI CAYNNNNRTG 3 cut(s) 1717, 1985, 2268
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 2 cut(s) 1047, 1164
SatI GCNGC 6 cut(s) 85, 1407, 1521, 1940, 1943, 2330
Sau3AI GATC 7 cut(s) 12, 387, 1585, 1902, 1909, 2153, 2257
Sau96I GGNCC 5 cut(s) 100, 1239, 1543, 1679, 2363
ScaI AGTACT 1 cut(s) 1592
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 6 cut(s) 118, 136, 179, 1281, 1311, 1467
SduI GDGCHC 4 cut(s) 125, 150, 1867, 2235
SfaNI GCATC 6 cut(s) 226, 1066, 1687, 1721, 2017, 2101
SfoI GGCGCC 1 cut(s) 176
SinI GGWCC 4 cut(s) 100, 1543, 1679, 2363
SmiMI CAYNNNNRTG 3 cut(s) 1717, 1985, 2268
SmlI CTYRAG 3 cut(s) 998, 1594, 1973
SmoI CTYRAG 3 cut(s) 998, 1594, 1973
SphI GCATGC 1 cut(s) 1972
SsiI CCGC 6 cut(s) 82, 157, 1237, 1325, 1373, 2210
SspDI GGCGCC 1 cut(s) 174
SspMI CTAG 7 cut(s) 77, 536, 1709, 1997, 2009, 2295, 2368
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 6 cut(s) 116, 134, 177, 1279, 1309, 1465
StyI CCWWGG 1 cut(s) 2149
TaaI ACNGT 2 cut(s) 1395, 1922
TaiI ACGT 1 cut(s) 1102
TatI WGTACW 2 cut(s) 1108, 1590
TfiI GAWTC 9 cut(s) 29, 686, 791, 896, 980, 1148, 1274, 1288, 1385
TscAI CASTG 6 cut(s) 1398, 1453, 1724, 1761, 1987, 2211
TseFI GTSAC 1 cut(s) 1342
TseI GCWGC 6 cut(s) 84, 1406, 1520, 1939, 1942, 2329
Tsp45I GTSAC 1 cut(s) 1342
TspRI CASTG 6 cut(s) 1398, 1453, 1724, 1761, 1987, 2211
Van91I CCANNNNNTGG 1 cut(s) 1676
Vha464I CTTAAG 1 cut(s) 1594
VpaK11BI GGWCC 4 cut(s) 100, 1543, 1679, 2363
XagI CCTNNNNNAGG 1 cut(s) 1034
XapI RAATTY 5 cut(s) 1183, 1226, 1483, 1750, 2049
XceI RCATGY 4 cut(s) 1676, 1889, 1972, 1990
XcmI CCANNNNNNNNNTGG 1 cut(s) 1753
XmiI GTMKAC 1 cut(s) 39
XmnI GAANNNNTTC 1 cut(s) 1499
XspI CTAG 7 cut(s) 77, 536, 1709, 1997, 2009, 2295, 2368
ZrmI AGTACT 1 cut(s) 1592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.