RLG00000025552

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000126
Physical Location & Seq
Reverse (-)
46632607 .. 46637064
4458 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025552

Sequence Viewer

Length: 3000 bp
ATGGAGAAGATAGACTCCAATTTGAAGGAATTTGAGTCGAAAGAGAGGAGTGTACGCAAACAACTGGAGACTTTACAGTCTCAATCTTCGTCTATGCTTCGGTTTTCTCTGCAGTGGAAGGAGTTACAGGACCGTTTGAAGTCGACACAGGCCCAAATCCAGACCCAGTTGGAGGAGGTAAAGAGGTGCGAGGCTGAGAAGGTGAGGCTGATGACGGAGGTGGAGACGAAAGCCAAGGAGTTGCGGAAAGTGGAGGAGTTGATTGATCAAGGTAAGACTCATCTGAATTATCTTGAGTCATTCATTGAGCAAAAGGGTCATGAGGTTGGTGAATGTGGTGAAAAAGATAGGGACAGGGATAGGGAGTCGAGTGTGGAGGAAGATAGGGTTGTGGATGGTGGGAATATAATTGCTTTGGTGATGAAGAGGTTGAAGGAGGCTGGGTTTGATGAGGTGAGCGAAGTGGAAGTGGCGAAGGGAGTGTTGGATAAATATTGTGGGGAAATCGAGTCGAAGAAGAGGGAGTTGAGTGTGATTTTGGGGGAGATTGAGAAGAAGAGGGAGGAGTTGGGTTTGCAAGAAGAGCAAATTAGGGTGGTGAAGAGTGCTATTAAGGGATGTGATAAGGAAATGAAGCAGAAAGAGGAGAAGCTGAATTTGATTCAGAAATCGATAGAGGAATGCGCGAATATGTTGGATGAGAAGGGGGAGATTGTTAGGGAAATGAGTGAGGAAGTGGAGGAATTTCGTGTGCGAAGGAAGGAAATGGATGAGTTGAAATGTAAACTTGAGGCGAAGGAGAGTGAGCTGGAGAGATGGGCAGTGGCCCTTGCATTGAAAGAGAAACAAGTTAAATCAAGATGTAAGGAACTTAGTTTGACTGAAAAGAGGGTCGTGGAAAGCCTCAGTGAGGTTCAGATGAAAGAAAAGGGTTTTGATGAGCAGAAAAAATTGATAGAAGAAGGTAGAAAGCATTTGGATGTAGTCTCCAATAAACTACAAATGAAAGAGTGGAGACTTCGTAAACAAGGAAAAGACCTTAACTTGGAAAAACAAGTATTGGAGTCGAATAAAAAATCCACTAAAGAACACAAACAGGCTCCAAAATCGCAAGAGGAGCCTAACGCCCTCCGTTCTGAATCTGAAATAACTTGGGATGGTATAGGCTTGCAGAGGTTCATGAGTGGGCAGTTGCGGAGAATAGATTTAATGGGTAGCAAAATGTCAGCTGTTCTTCAAGGCTCATCAGACCCGGCTAAATTAGTGTTGGCTGCAATGCAAGGATTTCACAGGTCAAATTCAAATACGGACAGCAGGGAGATTGAATTTGATTTGGAAATTACTAGAAGGAGTTGCATTCTTTTGCTGGAGGAGCTGAAGAGTATGTCAGCACAAATCAGTCCTCCAGTGAAAGAAGAAGCCATGAAGTTGGCCACTGAGTGGAAGTCTAAGATAGCAGAGACGACAATTAATAGATTAGAGATATTGGGGTTTCTGCAGCTTGTTACTACGTATGAGTTGACCTCTCTTTATGATGCAAATGAACTTCAAAGTCTTTTAGCTTTAATTGCTTCGCTTAAACAAGCAACTGAATGCCAGGCCCTTGCTATCTCTGATAAGCCATCTGGAATCAGTATGTGTCCTCTTGTGAAAGATGAGGAACAAGAATCTTCACTGGCCAGAAATGCAATAACTTCTTCGAACCTTCAACCAAGTGCCAGCGTACATGCAAGGAATTCTCCAGGGTTCGTAAATGATCAATTGGCTTGGAAACATTCAATACTGAACAAAAATTTTGTTGCTCTTGAAAAGTCATCAAATTTAAACCCAGCAAAACTTTTGTTGGATTTGATGCAAGTATCCTATGCTGGATACTGGACACAAGGAAATGCTGGTTTTGAAGCAACTGCCATGAAGAGCTTCATTTCCCTATTGGAGACTGTAAGAAGAGCCCAACCGCATGTTGGAACTCAGTTGAAAGAGGATGCAGCACAGTTAGCTGTACAATGGAAAAAGAAACTGACTGATGCTGAAAATCCCCTAGATATCTTGGGTTTCTTGTTGTTTATAGCTACGTATGGGTTACTTTCTACGTTCACGACAGATGAGCTTGCAAAGCTTTTTGGGCTGATTTCTCGACATACACATGCTTTAGAACTATGTCAGACAATTGGTTATGCACAGAAATTCCCTGATTATATTCTGGATCTTGTTCAAAGCAAACAACTAGTTGAGGCTGTCAGATTAATTTGCACCTCCAAATTAACAGACAAATTCCCCCCAGCACCACTGTTACGTGAATATGTGGAGGATTCATTGAATCGTTGCAGTAAAATTTGCACGACAAAGATACCACTTGATGAAAAGGATAAGGTTGTAGACGGCCTAATAGCTGATCTAAGATCTGTGATCCAATGCATCAACAAATACAATCTCGAGTCTGAATACCAATCCGCACATGTTAAAATACAAGTAGATGAGCTGAAAAAACTAAAGGAGAATTGGAGACTGTCGGCACAATCTCTTGCCTCCCAGGTTGAACCACAAAAGAGTAAAGGGAAGAAGCGTAGTTGTCCTCGCACTCCTACACCCATGTTTCAACCACAACAGCAGCTTGGACATAATGGTCAGCTGACAGCCATATCAGCTGCTAGACCGCCATCCTATCCATTGCCTACTTGTATCCCAAGCTATCCGCAGTCACATTCGTCATCTGTGCTGAAAACTAATTTTGGAATTCCCGGGCAGTTTACTATGCCTGCCAAGAGCCACCCATTTGCTGCTAATATTGGAGGGCCGTTCATGCCAACCATGAAGCGCCCGTGTATGATGACTACCAGATACCCAGCCTCAGGCACAGGCTCAGGCTCAGCCCCAGCCCCAGCCCCAGCTCCGGCTCCAGCTCCGGCCCCGGCTCCAACTCCAGTGCCAATGCACAGTGCTAATTTCCGTATACCATGCAAGTTTCCTCACCCTAATATGATGCAGTTTCGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

1000

Amino Acids

112.66

Weight (kDa)

8.18

Isoelectric Point (pI)

57.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 385 - 540 1.7e-31 Frigida-like protein
Frigida PF07899 580 - 835 7.4e-51 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 76, 2625
AccB7I CCANNNNNTGG 1 cut(s) 1440
AccI GTMKAC 3 cut(s) 143, 2379, 2953
AccII CGCG 1 cut(s) 686
AciI CCGC 6 cut(s) 244, 1195, 1958, 2454, 2657, 2696
AclWI GGATC 2 cut(s) 2214, 2404
AcoI YGGCCR 2 cut(s) 1431, 1677
AcuI CTGAAG 1 cut(s) 1397
AdeI CACNNNGTG 1 cut(s) 1440
AfaI GTAC 3 cut(s) 54, 1725, 2004
AfiI CCNNNNNNNGG 7 cut(s) 172, 910, 1045, 1440, 1964, 2852, 2893
AflIII ACRYGT 1 cut(s) 2458
AhlI ACTAGT 1 cut(s) 2227
AjnI CCWGG 3 cut(s) 1596, 1741, 2532
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
Alw26I GTCTC 8 cut(s) 62, 84, 218, 991, 1009, 1454, 1931, 2500
AlwI GGATC 2 cut(s) 2214, 2404
Ama87I CYCGRG 2 cut(s) 2435, 2741
AoxI GGCC 8 cut(s) 150, 825, 1431, 1599, 1677, 2383, 2795, 2907
ApeKI GCWGC 6 cut(s) 1271, 1498, 1988, 2611, 2648, 2780
AseI ATTAAT 2 cut(s) 1470, 2246
Asp700I GAANNNNTTC 1 cut(s) 1919
AspLEI GCGC 2 cut(s) 686, 2820
AspS9I GGNCC 6 cut(s) 130, 151, 826, 1600, 2795, 2908
AsuC2I CCSGG 4 cut(s) 1253, 2742, 2743, 2912
AsuHPI GGTGA 7 cut(s) 214, 341, 350, 430, 466, 610, 2963
AsuII TTCGAA 1 cut(s) 1700
AvaI CYCGRG 2 cut(s) 2435, 2741
AvaII GGWCC 1 cut(s) 130
AxyI CCTNAGG 1 cut(s) 2851
BalI TGGCCA 2 cut(s) 1433, 1679
BanII GRGCYC 1 cut(s) 1954
BbvI GCAGC 6 cut(s) 1258, 1510, 2000, 2623, 2635, 2767
BccI CCATC 5 cut(s) 389, 810, 1151, 1630, 2668
BceAI ACGGC 2 cut(s) 2398, 2782
BciT130I CCWGG 3 cut(s) 1598, 1743, 2534
BciVI GTATCC 3 cut(s) 1865, 1870, 2693
BclI TGATCA 2 cut(s) 265, 1756
BcnI CCSGG 4 cut(s) 1253, 2742, 2743, 2912
BcoDI GTCTC 8 cut(s) 62, 84, 218, 991, 1009, 1454, 1931, 2500
BcuI ACTAGT 1 cut(s) 2227
BfaI CTAG 4 cut(s) 1344, 2042, 2228, 2652
BfmI CTRYAG 2 cut(s) 110, 1496
BfoI RGCGCY 1 cut(s) 2821
BfuI GTATCC 3 cut(s) 1865, 1870, 2693
BglII AGATCT 1 cut(s) 2402
BisI GCNGC 6 cut(s) 1272, 1499, 1989, 2612, 2649, 2781
BlpI GCTNAGC 1 cut(s) 2869
BlsI GCNGC 6 cut(s) 1273, 1500, 1990, 2613, 2650, 2782
Bme1390I CCNGG 7 cut(s) 1253, 1598, 1743, 2534, 2742, 2743, 2912
Bme18I GGWCC 1 cut(s) 130
BmeT110I CYCGRG 2 cut(s) 2435, 2741
BmgT120I GGNCC 6 cut(s) 130, 151, 826, 1600, 2795, 2908
BmiI GGNNCC 5 cut(s) 1101, 1119, 2898, 2910, 2916
BmrFI CCNGG 7 cut(s) 1253, 1598, 1743, 2534, 2742, 2743, 2912
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 6 cut(s) 1525, 1842, 1975, 2017, 2427, 2973
BmuI ACTGGG 1 cut(s) 160
BplI GAGNNNNNCTC 2 cut(s) 1508, 1540
BpmI CTGGAG 7 cut(s) 86, 830, 1388, 1389, 1725, 2883, 2907
Bpu10I CCTNAGC 1 cut(s) 2863
Bpu1102I GCTNAGC 1 cut(s) 2869
Bpu14I TTCGAA 1 cut(s) 1700
BpuEI CTTGAG 2 cut(s) 314, 809
BpuMI CCSGG 4 cut(s) 1253, 2742, 2743, 2912
Bsa29I ATCGAT 1 cut(s) 671
BsaAI YACGTR 3 cut(s) 1512, 2076, 2297
BsaJI CCNNGG 5 cut(s) 234, 1742, 2532, 2741, 2910
BsaXI ACNNNNNCTCC 4 cut(s) 428, 458, 1006, 1036
Bsc4I CCNNNNNNNGG 7 cut(s) 172, 910, 1045, 1440, 1964, 2852, 2893
Bse1I ACTGG 6 cut(s) 69, 166, 1406, 1680, 1880, 2924
Bse21I CCTNAGG 1 cut(s) 2851
Bse3DI GCAATG 2 cut(s) 1281, 2669
BseBI CCWGG 3 cut(s) 1598, 1743, 2534
BseCI ATCGAT 1 cut(s) 671
BseDI CCNNGG 5 cut(s) 234, 1742, 2532, 2741, 2910
BseGI GGATG 8 cut(s) 400, 623, 703, 775, 985, 1162, 1990, 2660
BseLI CCNNNNNNNGG 7 cut(s) 172, 910, 1045, 1440, 1964, 2852, 2893
BseMI GCAATG 2 cut(s) 1281, 2669
BseMII CTCAG 7 cut(s) 186, 919, 1428, 1985, 2865, 2877, 2883
BseNI ACTGG 6 cut(s) 69, 166, 1406, 1680, 1880, 2924
BseRI GAGGAG 7 cut(s) 61, 188, 269, 578, 659, 1130, 1385
BseXI GCAGC 6 cut(s) 1258, 1510, 2000, 2623, 2635, 2767
BseYI CCCAGC 7 cut(s) 440, 1828, 2281, 2845, 2875, 2881, 2887
Bsh1236I CGCG 1 cut(s) 686
BshFI GGCC 8 cut(s) 152, 827, 1433, 1601, 1679, 2385, 2797, 2909
BshVI ATCGAT 1 cut(s) 671
BsiHKCI CYCGRG 2 cut(s) 2435, 2741
BsiSI CCGG 5 cut(s) 1253, 2742, 2894, 2906, 2912
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 7 cut(s) 172, 910, 1045, 1440, 1964, 2852, 2893
BsmAI GTCTC 8 cut(s) 62, 84, 218, 991, 1009, 1454, 1931, 2500
BsmBI CGTCTC 2 cut(s) 218, 1454
BsmFI GGGAC 1 cut(s) 365
BsmI GAATGC 3 cut(s) 686, 1356, 1598
BsnI GGCC 8 cut(s) 152, 827, 1433, 1601, 1679, 2385, 2797, 2909
BsoBI CYCGRG 2 cut(s) 2435, 2741
Bsp119I TTCGAA 1 cut(s) 1700
Bsp1286I GDGCHC 1 cut(s) 1954
Bsp1407I TGTACA 1 cut(s) 2002
Bsp143I GATC 6 cut(s) 265, 1756, 2206, 2395, 2402, 2409
Bsp1720I GCTNAGC 1 cut(s) 2869
BspACI CCGC 6 cut(s) 244, 1195, 1958, 2454, 2657, 2696
BspANI GGCC 8 cut(s) 152, 827, 1433, 1601, 1679, 2385, 2797, 2909
BspCNI CTCAG 7 cut(s) 187, 918, 1429, 1984, 2864, 2876, 2882
BspDI ATCGAT 1 cut(s) 671
BspFNI CGCG 1 cut(s) 686
BspHI TCATGA 2 cut(s) 319, 1179
BspLI GGNNCC 5 cut(s) 1101, 1119, 2898, 2910, 2916
BspMAI CTGCAG 2 cut(s) 114, 1500
BspPI GGATC 2 cut(s) 2214, 2404
BspQI GCTCTTC 3 cut(s) 576, 1910, 1942
BspT104I TTCGAA 1 cut(s) 1700
BsrDI GCAATG 2 cut(s) 1281, 2669
BsrGI TGTACA 1 cut(s) 2002
BsrI ACTGG 6 cut(s) 69, 166, 1406, 1680, 1880, 2924
BssECI CCNNGG 5 cut(s) 234, 1742, 2532, 2741, 2910
BssMI GATC 6 cut(s) 265, 1756, 2206, 2395, 2402, 2409
BssNAI GTATAC 1 cut(s) 2954
BssT1I CCWWGG 1 cut(s) 234
Bst1107I GTATAC 1 cut(s) 2954
Bst2UI CCWGG 3 cut(s) 1598, 1743, 2534
Bst4CI ACNGT 7 cut(s) 78, 134, 1942, 1995, 2292, 2511, 2939
Bst6I CTCTTC 8 cut(s) 419, 512, 551, 576, 596, 1373, 1910, 1942
BstAUI TGTACA 1 cut(s) 2002
BstBAI YACGTR 3 cut(s) 1512, 2076, 2297
BstBI TTCGAA 1 cut(s) 1700
BstC8I GCNNGC 4 cut(s) 1169, 1720, 2112, 2760
BstENI CCTNNNNNAGG 1 cut(s) 908
BstF5I GGATG 8 cut(s) 400, 623, 703, 775, 985, 1162, 1990, 2660
BstFNI CGCG 1 cut(s) 686
BstH2I RGCGCY 1 cut(s) 2821
BstHHI GCGC 2 cut(s) 686, 2820
BstKTI GATC 6 cut(s) 268, 1759, 2209, 2398, 2405, 2412
BstMAI GTCTC 8 cut(s) 62, 84, 218, 991, 1009, 1454, 1931, 2500
BstMBI GATC 6 cut(s) 265, 1756, 2206, 2395, 2402, 2409
BstNI CCWGG 3 cut(s) 1598, 1743, 2534
BstNSI RCATGY 4 cut(s) 1730, 1964, 2150, 2462
BstSCI CCNGG 7 cut(s) 1251, 1596, 1741, 2532, 2740, 2741, 2910
BstSFI CTRYAG 2 cut(s) 110, 1496
BstSNI TACGTA 2 cut(s) 1512, 2076
BstUI CGCG 1 cut(s) 686
BstV1I GCAGC 6 cut(s) 1258, 1510, 2000, 2623, 2635, 2767
BstX2I RGATCY 2 cut(s) 2206, 2402
BstXI CCANNNNNNTGG 1 cut(s) 1429
BstYI RGATCY 2 cut(s) 2206, 2402
BstZ17I GTATAC 1 cut(s) 2954
Bsu15I ATCGAT 1 cut(s) 671
Bsu36I CCTNAGG 1 cut(s) 2851
BsuI GTATCC 3 cut(s) 1865, 1870, 2693
BsuRI GGCC 8 cut(s) 152, 827, 1433, 1601, 1679, 2385, 2797, 2909
BsuTUI ATCGAT 1 cut(s) 671
BtsCI GGATG 8 cut(s) 400, 623, 703, 775, 985, 1162, 1990, 2660
BtsI GCAGTG 2 cut(s) 119, 828
BtsIMutI CAGTG 9 cut(s) 119, 828, 913, 1413, 1434, 1673, 2288, 2931, 2944
Cac8I GCNNGC 4 cut(s) 1169, 1720, 2112, 2760
CciI TCATGA 2 cut(s) 319, 1179
CfoI GCGC 2 cut(s) 686, 2820
Cfr13I GGNCC 6 cut(s) 130, 151, 826, 1600, 2795, 2908
Cfr9I CCCGGG 1 cut(s) 2741
ClaI ATCGAT 1 cut(s) 671
Csp6I GTAC 3 cut(s) 53, 1724, 2003
CviQI GTAC 3 cut(s) 53, 1724, 2003
DpnI GATC 6 cut(s) 267, 1758, 2208, 2397, 2404, 2411
DpnII GATC 6 cut(s) 265, 1756, 2206, 2395, 2402, 2409
DraI TTTAAA 1 cut(s) 1824
DraIII CACNNNGTG 1 cut(s) 1440
DrdI GACNNNNNNGTC 2 cut(s) 76, 2625
DseDI GACNNNNNNGTC 2 cut(s) 76, 2625
EaeI YGGCCR 2 cut(s) 1431, 1677
Eam1104I CTCTTC 8 cut(s) 419, 512, 551, 576, 596, 1373, 1910, 1942
EarI CTCTTC 8 cut(s) 419, 512, 551, 576, 596, 1373, 1910, 1942
Eco105I TACGTA 2 cut(s) 1512, 2076
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 1954
Eco32I GATATC 1 cut(s) 2047
Eco47I GGWCC 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 1397
Eco81I CCTNAGG 1 cut(s) 2851
Eco88I CYCGRG 2 cut(s) 2435, 2741
EcoNI CCTNNNNNAGG 1 cut(s) 908
EcoO109I RGGNCCY 1 cut(s) 1600
EcoRI GAATTC 2 cut(s) 1735, 2736
EcoRII CCWGG 3 cut(s) 1596, 1741, 2532
EcoRV GATATC 1 cut(s) 2047
EcoT14I CCWWGG 1 cut(s) 234
EcoT22I ATGCAT 1 cut(s) 2420
EcoT38I GRGCYC 1 cut(s) 1954
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 2 cut(s) 218, 1454
FaqI GGGAC 1 cut(s) 365
FbaI TGATCA 2 cut(s) 265, 1756
FblI GTMKAC 3 cut(s) 143, 2379, 2953
Fnu4HI GCNGC 6 cut(s) 1272, 1499, 1989, 2612, 2649, 2781
FokI GGATG 8 cut(s) 407, 630, 710, 782, 992, 1169, 1997, 2647
FriOI GRGCYC 1 cut(s) 1954
Fsp4HI GCNGC 6 cut(s) 1272, 1499, 1989, 2612, 2649, 2781
FspBI CTAG 4 cut(s) 1344, 2042, 2228, 2652
GlaI GCGC 2 cut(s) 685, 2819
GluI GCNGC 6 cut(s) 1272, 1499, 1989, 2612, 2649, 2781
GsaI CCCAGC 7 cut(s) 444, 1832, 2285, 2849, 2879, 2885, 2891
GsuI CTGGAG 7 cut(s) 86, 830, 1388, 1389, 1725, 2883, 2907
HaeII RGCGCY 1 cut(s) 2821
HaeIII GGCC 8 cut(s) 152, 827, 1433, 1601, 1679, 2385, 2797, 2909
HapII CCGG 5 cut(s) 1253, 2742, 2894, 2906, 2912
HhaI GCGC 2 cut(s) 686, 2820
Hin6I GCGC 2 cut(s) 684, 2818
HinP1I GCGC 2 cut(s) 684, 2818
HincII GTYRAC 2 cut(s) 144, 1521
HindII GTYRAC 2 cut(s) 144, 1521
HindIII AAGCTT 1 cut(s) 2117
HpaII CCGG 5 cut(s) 1253, 2742, 2894, 2906, 2912
HphI GGTGA 7 cut(s) 214, 341, 350, 430, 466, 610, 2963
Hpy166II GTNNAC 9 cut(s) 53, 144, 785, 1025, 1521, 2097, 2380, 2751, 2954
Hpy8I GTNNAC 9 cut(s) 53, 144, 785, 1025, 1521, 2097, 2380, 2751, 2954
HpyCH4III ACNGT 7 cut(s) 78, 134, 1942, 1995, 2292, 2511, 2939
HpyCH4IV ACGT 4 cut(s) 1511, 2075, 2093, 2296
HpySE526I ACGT 4 cut(s) 1511, 2075, 2093, 2296
HspAI GCGC 2 cut(s) 684, 2818
Ksp22I TGATCA 2 cut(s) 265, 1756
Kzo9I GATC 6 cut(s) 265, 1756, 2206, 2395, 2402, 2409
LguI GCTCTTC 3 cut(s) 576, 1910, 1942
LmnI GCTCC 7 cut(s) 1105, 1117, 1372, 2896, 2902, 2908, 2920
Lsp1109I GCAGC 6 cut(s) 1258, 1510, 2000, 2623, 2635, 2767
LweI GCATC 6 cut(s) 1525, 1842, 1975, 2017, 2427, 2973
MaeI CTAG 4 cut(s) 1344, 2042, 2228, 2652
MaeII ACGT 4 cut(s) 1511, 2075, 2093, 2296
MaeIII GTNAC 5 cut(s) 123, 1504, 2082, 2292, 2700
MalI GATC 6 cut(s) 267, 1758, 2208, 2397, 2404, 2411
MboI GATC 6 cut(s) 265, 1756, 2206, 2395, 2402, 2409
MfeI CAATTG 2 cut(s) 1760, 2169
MflI RGATCY 2 cut(s) 2206, 2402
MhlI GDGCHC 1 cut(s) 1954
MlsI TGGCCA 2 cut(s) 1433, 1679
MluNI TGGCCA 2 cut(s) 1433, 1679
MlyI GAGTC 8 cut(s) 8, 44, 271, 305, 374, 518, 1073, 2447
MmeI TCCRAC 6 cut(s) 150, 465, 675, 1824, 1945, 2942
Mox20I TGGCCA 2 cut(s) 1433, 1679
Mph1103I ATGCAT 1 cut(s) 2420
MroXI GAANNNNTTC 1 cut(s) 1919
MscI TGGCCA 2 cut(s) 1433, 1679
MslI CAYNNNNRTG 2 cut(s) 978, 2145
Msp20I TGGCCA 2 cut(s) 1433, 1679
MspA1I CMGCKG 3 cut(s) 1229, 2632, 2648
MspI CCGG 5 cut(s) 1253, 2742, 2894, 2906, 2912
MspR9I CCNGG 7 cut(s) 1253, 1598, 1743, 2534, 2742, 2743, 2912
MunI CAATTG 2 cut(s) 1760, 2169
Mva1269I GAATGC 3 cut(s) 686, 1356, 1598
MvaI CCWGG 3 cut(s) 1598, 1743, 2534
MvnI CGCG 1 cut(s) 686
NciI CCSGG 4 cut(s) 1253, 2742, 2743, 2912
NdeII GATC 6 cut(s) 265, 1756, 2206, 2395, 2402, 2409
NlaIV GGNNCC 5 cut(s) 1101, 1119, 2898, 2910, 2916
NmuCI GTSAC 1 cut(s) 2700
NsiI ATGCAT 1 cut(s) 2420
NspI RCATGY 4 cut(s) 1730, 1964, 2150, 2462
NspV TTCGAA 1 cut(s) 1700
PaeR7I CTCGAG 1 cut(s) 2435
PagI TCATGA 2 cut(s) 319, 1179
PciI ACATGT 1 cut(s) 2458
PciSI GCTCTTC 3 cut(s) 576, 1910, 1942
PctI GAATGC 3 cut(s) 686, 1356, 1598
PdmI GAANNNNTTC 1 cut(s) 1919
PfeI GAWTC 6 cut(s) 661, 1139, 1629, 1667, 2312, 2320
PflMI CCANNNNNTGG 1 cut(s) 1440
PkrI GCNGC 6 cut(s) 1273, 1500, 1990, 2613, 2650, 2782
PleI GAGTC 8 cut(s) 8, 43, 271, 304, 373, 517, 1072, 2446
PpsI GAGTC 8 cut(s) 8, 43, 271, 304, 373, 517, 1072, 2446
Ppu21I YACGTR 3 cut(s) 1512, 2076, 2297
PscI ACATGT 1 cut(s) 2458
PshBI ATTAAT 2 cut(s) 1470, 2246
Psp6I CCWGG 3 cut(s) 1596, 1741, 2532
PspFI CCCAGC 7 cut(s) 440, 1828, 2281, 2845, 2875, 2881, 2887
PspGI CCWGG 3 cut(s) 1596, 1741, 2532
PspN4I GGNNCC 5 cut(s) 1101, 1119, 2898, 2910, 2916
PspPI GGNCC 6 cut(s) 130, 151, 826, 1600, 2795, 2908
PstI CTGCAG 2 cut(s) 114, 1500
PsuI RGATCY 2 cut(s) 2206, 2402
PvuII CAGCTG 3 cut(s) 1229, 2632, 2648
RsaI GTAC 3 cut(s) 54, 1725, 2004
RsaNI GTAC 3 cut(s) 53, 1724, 2003
RseI CAYNNNNRTG 2 cut(s) 978, 2145
SalI GTCGAC 1 cut(s) 142
SapI GCTCTTC 3 cut(s) 576, 1910, 1942
SatI GCNGC 6 cut(s) 1272, 1499, 1989, 2612, 2649, 2781
Sau3AI GATC 6 cut(s) 265, 1756, 2206, 2395, 2402, 2409
Sau96I GGNCC 6 cut(s) 130, 151, 826, 1600, 2795, 2908
SchI GAGTC 8 cut(s) 8, 44, 271, 305, 374, 518, 1073, 2447
ScrFI CCNGG 7 cut(s) 1253, 1598, 1743, 2534, 2742, 2743, 2912
SduI GDGCHC 1 cut(s) 1954
SfaNI GCATC 6 cut(s) 1525, 1842, 1975, 2017, 2427, 2973
SfcI CTRYAG 2 cut(s) 110, 1496
Sfr274I CTCGAG 1 cut(s) 2435
SfuI TTCGAA 1 cut(s) 1700
SinI GGWCC 1 cut(s) 130
SlaI CTCGAG 1 cut(s) 2435
SmaI CCCGGG 1 cut(s) 2743
SmiMI CAYNNNNRTG 2 cut(s) 978, 2145
SmlI CTYRAG 3 cut(s) 293, 788, 2435
SmoI CTYRAG 3 cut(s) 293, 788, 2435
SnaBI TACGTA 2 cut(s) 1512, 2076
SpeI ACTAGT 1 cut(s) 2227
SsiI CCGC 6 cut(s) 244, 1195, 1958, 2454, 2657, 2696
SspI AATATT 2 cut(s) 494, 2788
SspMI CTAG 4 cut(s) 1344, 2042, 2228, 2652
StyD4I CCNGG 7 cut(s) 1251, 1596, 1741, 2532, 2740, 2741, 2910
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 7 cut(s) 78, 134, 1942, 1995, 2292, 2511, 2939
TaiI ACGT 4 cut(s) 1514, 2078, 2096, 2299
TatI WGTACW 1 cut(s) 2002
TfiI GAWTC 6 cut(s) 661, 1139, 1629, 1667, 2312, 2320
TscAI CASTG 9 cut(s) 119, 828, 913, 1413, 1441, 1680, 2295, 2931, 2944
TseFI GTSAC 1 cut(s) 2700
TseI GCWGC 6 cut(s) 1271, 1498, 1988, 2611, 2648, 2780
Tsp45I GTSAC 1 cut(s) 2700
TspGWI ACGGA 4 cut(s) 230, 1121, 1322, 2939
TspMI CCCGGG 1 cut(s) 2741
TspRI CASTG 9 cut(s) 119, 828, 913, 1413, 1441, 1680, 2295, 2931, 2944
Van91I CCANNNNNTGG 1 cut(s) 1440
VpaK11BI GGWCC 1 cut(s) 130
VspI ATTAAT 2 cut(s) 1470, 2246
XagI CCTNNNNNAGG 1 cut(s) 908
XceI RCATGY 4 cut(s) 1730, 1964, 2150, 2462
XcmI CCANNNNNNNNNTGG 1 cut(s) 1961
XhoI CTCGAG 1 cut(s) 2435
XmaI CCCGGG 1 cut(s) 2741
XmiI GTMKAC 3 cut(s) 143, 2379, 2953
XmnI GAANNNNTTC 1 cut(s) 1919
XspI CTAG 4 cut(s) 1344, 2042, 2228, 2652
Zsp2I ATGCAT 1 cut(s) 2420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.