Rroxscaffold_6G00430040

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000126
Physical Location & Seq
Forward (+)
50246956 .. 50251067
4112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430040.1

Sequence Viewer

Length: 2760 bp
ATGGAGAAGATAGCATCCGATTTGAAAGTATCGGAGTTGAAGCAAAGGAGTCTAGGCAAGGCGTACGAGACCTTGCATTCCCAAGCGTCTTCCATGCTCGTTCTCAGCATTCAATGGAAGGACCTTCAGGAACACTTCGATTCGACCCGGAATGCGATCAGGACTCGTTTCGAAGAGCTTCAGGAACGAGAGAAGCAGTTGGAAGCCAAGGAGTCAAACTTGAAGTCAGAGATGGAATCCAAGAAGGACGAGTTGTTTGGAGTTGAGAAGCTGATTGATGAGAAAGTACAGGAAGTTATAGAAATAAAGAGATCGATTCAAAAGCACAGTGAGGAGATTGAACGTGAAGAGAAGAGAGTCTTGGAAGTACAGAAATTGGTTATAGACAAGGAGAGGGAGTGTAGATTGATCGAAAGACGGATTCAAGAGAGTGGGAAGAAACTGAGTTGGGTTGAGAGAAGAACTGAAGAGAAATTGAACGAGGTTGAGTGTAAGGAGAAGGAACTGAATAAGTACCGTGAGGATGCTAAGTTGAAAGTGGAGCAACTAAGTGTGATTGATGGGGCAATTGTGGAGCGTAACGAAATCATCGAGTTAAAAGAGGAGAAATTGAGTTGGATTGAGAAGAGTATTGGAGAGAAATCCAAGTTGGCCAACTCTAAGGAGGAGGAAGTGAGAGTGATACAACGGTCGTTGAATGTATACCGTGAGAGTATCAAGTCAAAAGAGAGTGACTTAGATGCGATTCTTGAGTCGATTGAAGGGAAGAAGAAAGAATTTGATGTGAAAGAAGAGCAAATTAAAACGTTGCAGAGATTGATTGAGGGATGTGAGAAAGAGCTCAAATTGAAAGAGGAGAAATTGAAGGAGGAAACACTGAAAGTGAAAGAATACAGTTTGCGAAATATACAAATGGAGGAGTGGTCCTGCAAGCTTGAATTTAAAGAGAGGGAAGTAGAATTGAAAGAGAAACGATTAGAGTCAAAAATGGAGGAATTTAAAAAATGGGTTGAAGAACGAGGAAAGGATTTGAATTCACTCTCCTCGGAACTGAAAGTTAAAGAGTGTCAACTTGAACAGGCGGCCAAAGAGCTTGAGTTGACTAAGAAACAATGTGGTGTTGTTGCTCAAGTGAAGATTGGGCCATTGGAAAAGACTCCAGAGAACGATGCCATTGTTTCTTCATCTGCAAGTGATCAATCCAACATCAATATCACCGACGGTAGAAGTTTGCAGTTGTTCATGTATGAGCATATGAAGAGAAATGATTCAATAAGCAACAAGATCTCATCCTTTCTCCAACAAAAGTCATCAGAACCAGCAAAATTGGTTTTGGATGCAATGCAAGGGTTTTACACTTCAAATTTGACTGTCGAGAACAAGGAGTTTGATTTGGTGCTTATTAGAAGGACATGCATTCTTTTGTTGGAAGAATTAAAGAGAGTCTCACCCCAAACTAATCCAGAGGTCAGAGAAGAAGCAATGAAGTTGGCCAGTGATTGGAAGGCTAAGATGAAAGTGGGGAGTGAGAACTGGTTGGAGATTTTGGGTTTTTTGCGGCTTGTTACTACTTATGGATTGACCTCTGCTTATAATGAGAAGGAGCTTCAGAGTCTTCTTGATATAGTTGCTCAGCATGAACAAGCAACAGAATTATCACGGGCCCTTGCTACCACAGATAAGGCACATGCAAATAGCAACATCTGTTCTCCTATGAAAATTGAAAAAACAAAATCTCCAGTAGCCCAAAATGAAGCAACCATCTCTTCTCCAAATCTTAAGCAAGCTGCCACCACTATTGACGCAAGGAACTTGCAGGGGTTTCAAAATGAGCAGTTTCGTGAAGCCTGTGATTCTGCTGATTCTGCTTTGAAAAGTATTATTTATGATTTTCTTGGAAAGAAGAGACTGATTGAGGCTGTTGGGTTGATTTACACCATGAAGTTATTTGACAAGTTTCCCCCAGTACAAATCTTAAAAGAATATGTTGATAATGGAAAGAGGTGCTGCAGGAATAGAACCAAGAGAAAGAAATCGCTGGATGAAAAGGATATGATTACAGACCAGCATATAGCAGATCTACAAGTTGTTATTCAATGTATCAAAGATTACAACCTCGAGTCTGAATGTCCACCTGAAGAAATCGAATCACAAATTGCTGCGCTTGAAATTGTTAAGGAGCGGAGACGTTTAGCTTCATCTTCTCTTGTTTTAAAGGTTGAACAACAGCAAAAAGTGCTGAAAAATGTTGAAGTACAAGAACAACAAGAGCAGAAAGCCTCCAAGGGTGAACAACAAGAACAGCAAAAAGAACAGAAAACCTCTGAGGCTAAACTACAAGAAAAAGTACAGCAGAAAGCCTCCAATAAGGTTGAAGAACAACAACAGCGACAAGAGAAAAACGCCTCCAAGGTTAAACAGCAAGAACAACAAGAGCAGAAAGCCTCCAAAGTTAAACAACTGCAGAAAAAAGGGAATAAACGTCCACACAGCACATTTGCCCCCAGGTTTGATCACCAACAGCAGTGTAAAAATAAATTTCAAAGAACATCTATATTAGCTGACAGACACCCGGACCACCCTGACTATCCGAATCCAAGTTCATCAACTTGGACACATGAAAGTAATGGATACCGTGAACAGTTTGGTACTGCCGTCAACAAGTATGAAGTTCATGCTAATTATGAACATCCTGTGCAGTTTGGTATTCCTGCCAATGGTAGTGCTATTTATGCATACGGTGGACAAATCTTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

919

Amino Acids

106.52

Weight (kDa)

6.52

Isoelectric Point (pI)

58.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 407 - 561 6.3e-32 Frigida-like protein
Frigida PF07899 623 - 730 7e-21 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1593
AccB7I CCANNNNNTGG 1 cut(s) 1500
AccBSI CCGCTC 1 cut(s) 2182
AccI GTMKAC 1 cut(s) 702
AciI CCGC 3 cut(s) 1082, 1558, 2182
AclI AACGTT 1 cut(s) 806
AcoI YGGCCR 3 cut(s) 651, 1083, 1491
AcsI RAATTY 6 cut(s) 776, 938, 995, 1033, 1363, 2537
AcuI CTGAAG 5 cut(s) 110, 164, 486, 1592, 2157
AfaI GTAC 8 cut(s) 65, 288, 369, 515, 1968, 2256, 2349, 2650
AfiI CCNNNNNNNGG 2 cut(s) 1500, 2717
AflII CTTAAG 1 cut(s) 1778
AjnI CCWGG 1 cut(s) 2504
AjuI GAANNNNNNNTTGG 4 cut(s) 344, 376, 632, 664
AluBI AGCT 9 cut(s) 178, 271, 841, 934, 1093, 1606, 1787, 2195, 2561
AluI AGCT 9 cut(s) 178, 271, 841, 934, 1093, 1606, 1787, 2195, 2561
Alw21I GWGCWC 1 cut(s) 843
Alw26I GTCTC 4 cut(s) 62, 1450, 1900, 2179
Ama87I CYCGRG 1 cut(s) 2117
AoxI GGCC 5 cut(s) 651, 1083, 1142, 1491, 1662
ApaI GGGCCC 1 cut(s) 1666
ApeKI GCWGC 3 cut(s) 1787, 2007, 2159
ApoI RAATTY 6 cut(s) 776, 938, 995, 1033, 1363, 2537
ArsI GACNNNNNNTTYG 4 cut(s) 209, 239, 241, 271
Asp700I GAANNNNTTC 2 cut(s) 177, 1267
AspLEI GCGC 1 cut(s) 2164
AspS9I GGNCC 6 cut(s) 121, 924, 1142, 1662, 1663, 2575
AsuC2I CCSGG 2 cut(s) 148, 2573
AsuHPI GGTGA 4 cut(s) 1207, 1440, 2300, 2507
AsuII TTCGAA 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 2117
AvaII GGWCC 3 cut(s) 121, 924, 2575
BaeGI GKGCMC 1 cut(s) 1666
BaeI ACNNNNGTAYC 4 cut(s) 674, 707, 2640, 2673
BalI TGGCCA 2 cut(s) 653, 1493
BanII GRGCYC 2 cut(s) 843, 1666
BbsI GAAGAC 2 cut(s) 81, 1607
Bbv12I GWGCWC 1 cut(s) 843
BbvI GCAGC 3 cut(s) 1774, 1994, 2146
BccI CCATC 3 cut(s) 226, 554, 1769
BceAI ACGGC 1 cut(s) 2639
BciT130I CCWGG 1 cut(s) 2506
BciVI GTATCC 1 cut(s) 2624
BclI TGATCA 2 cut(s) 1195, 2512
BcnI CCSGG 2 cut(s) 148, 2573
BcoDI GTCTC 4 cut(s) 62, 1450, 1900, 2179
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 2 cut(s) 2008, 2462
BfrI CTTAAG 1 cut(s) 1778
BfuI GTATCC 1 cut(s) 2624
BglII AGATCT 2 cut(s) 1284, 2077
BisI GCNGC 5 cut(s) 1083, 1559, 1788, 2008, 2160
BlpI GCTNAGC 1 cut(s) 1632
BlsI GCNGC 5 cut(s) 1084, 1560, 1789, 2009, 2161
Bme1390I CCNGG 3 cut(s) 148, 2506, 2573
Bme18I GGWCC 3 cut(s) 121, 924, 2575
BmeT110I CYCGRG 1 cut(s) 2117
BmgT120I GGNCC 6 cut(s) 121, 924, 1142, 1662, 1663, 2575
BmiI GGNNCC 1 cut(s) 1664
BmrFI CCNGG 3 cut(s) 148, 2506, 2573
BmrI ACTGGG 1 cut(s) 1958
BmsI GCATC 5 cut(s) 23, 514, 730, 1159, 1327
BmuI ACTGGG 1 cut(s) 1958
BpiI GAAGAC 2 cut(s) 81, 1607
BpmI CTGGAG 2 cut(s) 1143, 1722
Bpu1102I GCTNAGC 1 cut(s) 1632
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 3 cut(s) 770, 1113, 1115
BpuMI CCSGG 2 cut(s) 148, 2573
Bsa29I ATCGAT 1 cut(s) 314
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 5 cut(s) 207, 1044, 2283, 2409, 2504
BsaXI ACNNNNNCTCC 6 cut(s) 383, 413, 908, 938, 1720, 1750
Bsc4I CCNNNNNNNGG 2 cut(s) 1500, 2717
Bse1I ACTGG 4 cut(s) 1494, 1538, 1739, 1964
Bse3DI GCAATG 2 cut(s) 1347, 1488
BseBI CCWGG 1 cut(s) 2506
BseCI ATCGAT 1 cut(s) 314
BseDI CCNNGG 5 cut(s) 207, 1044, 2283, 2409, 2504
BseGI GGATG 7 cut(s) 14, 529, 833, 1289, 1342, 2047, 2689
BseLI CCNNNNNNNGG 2 cut(s) 1500, 2717
BseMI GCAATG 2 cut(s) 1347, 1488
BseMII CTCAG 4 cut(s) 118, 434, 1646, 2316
BseNI ACTGG 4 cut(s) 1494, 1538, 1739, 1964
BseRI GAGGAG 6 cut(s) 347, 617, 680, 869, 932, 1033
BseSI GKGCMC 1 cut(s) 1666
BseXI GCAGC 3 cut(s) 1774, 1994, 2146
BsgI GTGCAG 1 cut(s) 2717
Bsh1285I CGRYCG 1 cut(s) 692
BshFI GGCC 5 cut(s) 653, 1085, 1144, 1493, 1664
BshVI ATCGAT 1 cut(s) 314
BsiEI CGRYCG 1 cut(s) 692
BsiHKAI GWGCWC 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 2117
BsiSI CCGG 2 cut(s) 148, 2573
BsiWI CGTACG 1 cut(s) 63
BslI CCNNNNNNNGG 2 cut(s) 1500, 2717
BsmAI GTCTC 4 cut(s) 62, 1450, 1900, 2179
BsmBI CGTCTC 1 cut(s) 2179
BsmI GAATGC 4 cut(s) 76, 108, 157, 1416
BsnI GGCC 5 cut(s) 653, 1085, 1144, 1493, 1664
Bso31I GGTCTC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 2117
Bsp119I TTCGAA 1 cut(s) 171
Bsp120I GGGCCC 1 cut(s) 1662
Bsp1286I GDGCHC 2 cut(s) 843, 1666
Bsp143I GATC 7 cut(s) 156, 311, 408, 1195, 1284, 2077, 2512
Bsp1720I GCTNAGC 1 cut(s) 1632
BspACI CCGC 3 cut(s) 1082, 1558, 2182
BspANI GGCC 5 cut(s) 653, 1085, 1144, 1493, 1664
BspCNI CTCAG 4 cut(s) 117, 435, 1645, 2317
BspDI ATCGAT 1 cut(s) 314
BspLI GGNNCC 1 cut(s) 1664
BspMAI CTGCAG 2 cut(s) 2012, 2466
BspQI GCTCTTC 2 cut(s) 168, 786
BspT104I TTCGAA 1 cut(s) 171
BspTI CTTAAG 1 cut(s) 1778
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 2182
BsrDI GCAATG 2 cut(s) 1347, 1488
BsrI ACTGG 4 cut(s) 1494, 1538, 1739, 1964
BssECI CCNNGG 5 cut(s) 207, 1044, 2283, 2409, 2504
BssMI GATC 7 cut(s) 156, 311, 408, 1195, 1284, 2077, 2512
BssNAI GTATAC 1 cut(s) 703
BssT1I CCWWGG 3 cut(s) 207, 2283, 2409
Bst1107I GTATAC 1 cut(s) 703
Bst2UI CCWGG 1 cut(s) 2506
Bst6I CTCTTC 9 cut(s) 168, 342, 347, 462, 620, 786, 1253, 1771, 1898
BstAFI CTTAAG 1 cut(s) 1778
BstAPI GCANNNNNTGC 1 cut(s) 2236
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 932, 1785
BstF5I GGATG 7 cut(s) 14, 529, 833, 1289, 1342, 2047, 2689
BstHHI GCGC 1 cut(s) 2164
BstKTI GATC 7 cut(s) 159, 314, 411, 1198, 1287, 2080, 2515
BstMAI GTCTC 4 cut(s) 62, 1450, 1900, 2179
BstMBI GATC 7 cut(s) 156, 311, 408, 1195, 1284, 2077, 2512
BstMCI CGRYCG 1 cut(s) 692
BstMWI GCNNNNNNNGC 3 cut(s) 1865, 2236, 2732
BstNI CCWGG 1 cut(s) 2506
BstNSI RCATGY 2 cut(s) 1416, 1691
BstSCI CCNGG 3 cut(s) 146, 2504, 2571
BstSFI CTRYAG 2 cut(s) 2008, 2462
BstSLI GKGCMC 1 cut(s) 1666
BstV1I GCAGC 3 cut(s) 1774, 1994, 2146
BstV2I GAAGAC 2 cut(s) 81, 1607
BstX2I RGATCY 2 cut(s) 1284, 2077
BstYI RGATCY 2 cut(s) 1284, 2077
BstZ17I GTATAC 1 cut(s) 703
Bsu15I ATCGAT 1 cut(s) 314
BsuI GTATCC 1 cut(s) 2624
BsuRI GGCC 5 cut(s) 653, 1085, 1144, 1493, 1664
BsuTUI ATCGAT 1 cut(s) 314
BtsCI GGATG 7 cut(s) 14, 529, 833, 1289, 1342, 2047, 2689
BtsI GCAGTG 1 cut(s) 2531
BtsIMutI CAGTG 4 cut(s) 334, 875, 1501, 2531
Cac8I GCNNGC 2 cut(s) 932, 1785
CfoI GCGC 1 cut(s) 2164
Cfr13I GGNCC 6 cut(s) 121, 924, 1142, 1662, 1663, 2575
ClaI ATCGAT 1 cut(s) 314
CseI GACGC 2 cut(s) 75, 1811
Csp6I GTAC 8 cut(s) 64, 287, 368, 514, 1967, 2255, 2348, 2649
CspCI CAANNNNNGTGG 2 cut(s) 1780, 1815
CviAII CATG 8 cut(s) 94, 1243, 1413, 1637, 1688, 1939, 2618, 2675
CviQI GTAC 8 cut(s) 64, 287, 368, 514, 1967, 2255, 2348, 2649
DpnI GATC 7 cut(s) 158, 313, 410, 1197, 1286, 2079, 2514
DpnII GATC 7 cut(s) 156, 311, 408, 1195, 1284, 2077, 2512
DraI TTTAAA 3 cut(s) 943, 1000, 2214
EaeI YGGCCR 3 cut(s) 651, 1083, 1491
Eam1104I CTCTTC 9 cut(s) 168, 342, 347, 462, 620, 786, 1253, 1771, 1898
EarI CTCTTC 9 cut(s) 168, 342, 347, 462, 620, 786, 1253, 1771, 1898
Ecl136II GAGCTC 1 cut(s) 841
Eco130I CCWWGG 3 cut(s) 207, 2283, 2409
Eco24I GRGCYC 2 cut(s) 843, 1666
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 3 cut(s) 121, 924, 2575
Eco53kI GAGCTC 1 cut(s) 841
Eco57I CTGAAG 5 cut(s) 110, 164, 486, 1592, 2157
Eco88I CYCGRG 1 cut(s) 2117
EcoICRI GAGCTC 1 cut(s) 841
EcoO109I RGGNCCY 2 cut(s) 121, 1663
EcoRI GAATTC 1 cut(s) 1033
EcoRII CCWGG 1 cut(s) 2504
EcoT14I CCWWGG 3 cut(s) 207, 2283, 2409
EcoT22I ATGCAT 2 cut(s) 1418, 2737
EcoT38I GRGCYC 2 cut(s) 843, 1666
ErhI CCWWGG 3 cut(s) 207, 2283, 2409
Esp3I CGTCTC 1 cut(s) 2179
FaeI CATG 8 cut(s) 97, 1246, 1416, 1640, 1691, 1942, 2621, 2678
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FatI CATG 8 cut(s) 93, 1242, 1412, 1636, 1687, 1938, 2617, 2674
FauNDI CATATG 1 cut(s) 1254
FbaI TGATCA 2 cut(s) 1195, 2512
FblI GTMKAC 1 cut(s) 702
Fnu4HI GCNGC 5 cut(s) 1083, 1559, 1788, 2008, 2160
FokI GGATG 6 cut(s) 536, 840, 1276, 1349, 2054, 2676
FriOI GRGCYC 2 cut(s) 843, 1666
Fsp4HI GCNGC 5 cut(s) 1083, 1559, 1788, 2008, 2160
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 2163
GluI GCNGC 5 cut(s) 1083, 1559, 1788, 2008, 2160
GsuI CTGGAG 2 cut(s) 1143, 1722
HaeIII GGCC 5 cut(s) 653, 1085, 1144, 1493, 1664
HapII CCGG 2 cut(s) 148, 2573
HgaI GACGC 2 cut(s) 75, 1811
HhaI GCGC 1 cut(s) 2164
Hin1II CATG 8 cut(s) 97, 1246, 1416, 1640, 1691, 1942, 2621, 2678
Hin6I GCGC 1 cut(s) 2162
HinP1I GCGC 1 cut(s) 2162
HincII GTYRAC 3 cut(s) 1070, 1101, 2659
HindII GTYRAC 3 cut(s) 1070, 1101, 2659
HindIII AAGCTT 1 cut(s) 932
HpaII CCGG 2 cut(s) 148, 2573
HphI GGTGA 4 cut(s) 1207, 1440, 2300, 2507
Hpy166II GTNNAC 9 cut(s) 703, 1070, 1101, 2132, 2291, 2486, 2639, 2659, 2744
Hpy8I GTNNAC 9 cut(s) 703, 1070, 1101, 2132, 2291, 2486, 2639, 2659, 2744
Hpy99I CGWCG 1 cut(s) 1223
HpyAV CCTTC 9 cut(s) 112, 134, 238, 493, 755, 859, 1401, 1498, 1594
HpyCH4IV ACGT 4 cut(s) 343, 806, 2188, 2482
HpyF10VI GCNNNNNNNGC 3 cut(s) 1865, 2236, 2732
HpySE526I ACGT 4 cut(s) 343, 806, 2188, 2482
Hsp92II CATG 8 cut(s) 97, 1246, 1416, 1640, 1691, 1942, 2621, 2678
HspAI GCGC 1 cut(s) 2162
Ksp22I TGATCA 2 cut(s) 1195, 2512
Kzo9I GATC 7 cut(s) 156, 311, 408, 1195, 1284, 2077, 2512
LguI GCTCTTC 2 cut(s) 168, 786
LmnI GCTCC 4 cut(s) 541, 574, 1603, 2179
Lsp1109I GCAGC 3 cut(s) 1774, 1994, 2146
LweI GCATC 5 cut(s) 23, 514, 730, 1159, 1327
MaeI CTAG 1 cut(s) 53
MaeII ACGT 4 cut(s) 343, 806, 2188, 2482
MaeIII GTNAC 3 cut(s) 578, 731, 1564
MalI GATC 7 cut(s) 158, 313, 410, 1197, 1286, 2079, 2514
MbiI CCGCTC 1 cut(s) 2182
MboI GATC 7 cut(s) 156, 311, 408, 1195, 1284, 2077, 2512
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 2 cut(s) 1284, 2077
MhlI GDGCHC 2 cut(s) 843, 1666
MlsI TGGCCA 2 cut(s) 653, 1493
MluNI TGGCCA 2 cut(s) 653, 1493
MmeI TCCRAC 6 cut(s) 180, 596, 1227, 1324, 1407, 1518
Mox20I TGGCCA 2 cut(s) 653, 1493
Mph1103I ATGCAT 2 cut(s) 1418, 2737
MroXI GAANNNNTTC 2 cut(s) 177, 1267
MscI TGGCCA 2 cut(s) 653, 1493
Msp20I TGGCCA 2 cut(s) 653, 1493
MspCI CTTAAG 1 cut(s) 1778
MspI CCGG 2 cut(s) 148, 2573
MspR9I CCNGG 3 cut(s) 148, 2506, 2573
MunI CAATTG 1 cut(s) 567
Mva1269I GAATGC 4 cut(s) 76, 108, 157, 1416
MvaI CCWGG 1 cut(s) 2506
MwoI GCNNNNNNNGC 3 cut(s) 1865, 2236, 2732
NciI CCSGG 2 cut(s) 148, 2573
NdeI CATATG 1 cut(s) 1254
NdeII GATC 7 cut(s) 156, 311, 408, 1195, 1284, 2077, 2512
NlaIII CATG 8 cut(s) 97, 1246, 1416, 1640, 1691, 1942, 2621, 2678
NlaIV GGNNCC 1 cut(s) 1664
NmuCI GTSAC 1 cut(s) 731
NsiI ATGCAT 2 cut(s) 1418, 2737
NspI RCATGY 2 cut(s) 1416, 1691
NspV TTCGAA 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 2117
PciSI GCTCTTC 2 cut(s) 168, 786
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PctI GAATGC 4 cut(s) 76, 108, 157, 1416
PdmI GAANNNNTTC 2 cut(s) 177, 1267
Pfl23II CGTACG 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 1500
PkrI GCNGC 5 cut(s) 1084, 1560, 1789, 2009, 2161
PpuMI RGGWCCY 1 cut(s) 121
PsiI TTATAA 1 cut(s) 1593
Psp124BI GAGCTC 1 cut(s) 843
Psp1406I AACGTT 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 2504
PspGI CCWGG 1 cut(s) 2504
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 1664
PspOMI GGGCCC 1 cut(s) 1662
PspPI GGNCC 6 cut(s) 121, 924, 1142, 1662, 1663, 2575
PspPPI RGGWCCY 1 cut(s) 121
PspXI VCTCGAGB 1 cut(s) 2117
PstI CTGCAG 2 cut(s) 2012, 2466
PsuI RGATCY 2 cut(s) 1284, 2077
RsaI GTAC 8 cut(s) 65, 288, 369, 515, 1968, 2256, 2349, 2650
RsaNI GTAC 8 cut(s) 64, 287, 368, 514, 1967, 2255, 2348, 2649
SacI GAGCTC 1 cut(s) 843
SapI GCTCTTC 2 cut(s) 168, 786
SatI GCNGC 5 cut(s) 1083, 1559, 1788, 2008, 2160
Sau3AI GATC 7 cut(s) 156, 311, 408, 1195, 1284, 2077, 2512
Sau96I GGNCC 6 cut(s) 121, 924, 1142, 1662, 1663, 2575
ScrFI CCNGG 3 cut(s) 148, 2506, 2573
SduI GDGCHC 2 cut(s) 843, 1666
SfaNI GCATC 5 cut(s) 23, 514, 730, 1159, 1327
SfcI CTRYAG 2 cut(s) 2008, 2462
Sfr274I CTCGAG 1 cut(s) 2117
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 3 cut(s) 121, 924, 2575
SlaI CTCGAG 1 cut(s) 2117
SmlI CTYRAG 5 cut(s) 749, 1094, 1128, 1778, 2117
SmoI CTYRAG 5 cut(s) 749, 1094, 1128, 1778, 2117
SsiI CCGC 3 cut(s) 1082, 1558, 2182
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 1 cut(s) 843
StyD4I CCNGG 3 cut(s) 146, 2504, 2571
StyI CCWWGG 3 cut(s) 207, 2283, 2409
TaiI ACGT 4 cut(s) 346, 809, 2191, 2485
TatI WGTACW 5 cut(s) 286, 367, 1966, 2254, 2347
TauI GCSGC 2 cut(s) 1085, 1561
TscAI CASTG 4 cut(s) 334, 882, 1501, 2531
TseFI GTSAC 1 cut(s) 731
TseI GCWGC 3 cut(s) 1787, 2007, 2159
Tsp45I GTSAC 1 cut(s) 731
TspGWI ACGGA 1 cut(s) 433
TspRI CASTG 4 cut(s) 334, 882, 1501, 2531
Van91I CCANNNNNTGG 1 cut(s) 1500
Vha464I CTTAAG 1 cut(s) 1778
VpaK11BI GGWCC 3 cut(s) 121, 924, 2575
XapI RAATTY 6 cut(s) 776, 938, 995, 1033, 1363, 2537
XceI RCATGY 2 cut(s) 1416, 1691
XhoI CTCGAG 1 cut(s) 2117
XmiI GTMKAC 1 cut(s) 702
XmnI GAANNNNTTC 2 cut(s) 177, 1267
XspI CTAG 1 cut(s) 53
Zsp2I ATGCAT 2 cut(s) 1418, 2737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.