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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 2609 |
| Acc36I | ACCTGC | 1 cut(s) | 2445 |
| Acc65I | GGTACC | 1 cut(s) | 2644 |
| AccB1I | GGYRCC | 3 cut(s) | 1633, 2644, 2725 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 874 |
| AciI | CCGC | 5 cut(s) | 10, 121, 1473, 1507, 2260 |
| AclWI | GGATC | 1 cut(s) | 1163 |
| AcsI | RAATTY | 3 cut(s) | 601, 808, 2738 |
| AcuI | CTGAAG | 2 cut(s) | 2166, 2731 |
| AfaI | GTAC | 3 cut(s) | 963, 2646, 2689 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 790, 874, 916 |
| AflIII | ACRYGT | 2 cut(s) | 2071, 2416 |
| AhlI | ACTAGT | 1 cut(s) | 727 |
| AjnI | CCWGG | 4 cut(s) | 660, 1151, 1636, 2716 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1895, 1927 |
| Alw26I | GTCTC | 4 cut(s) | 547, 718, 2268, 2752 |
| AlwI | GGATC | 1 cut(s) | 1163 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1940 |
| Ama87I | CYCGRG | 1 cut(s) | 2549 |
| AoxI | GGCC | 3 cut(s) | 658, 699, 1035 |
| ApeKI | GCWGC | 8 cut(s) | 7, 623, 878, 1109, 1442, 1595, 1808, 2519 |
| ApoI | RAATTY | 3 cut(s) | 601, 808, 2738 |
| AseI | ATTAAT | 1 cut(s) | 1407 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2245 |
| Asp718I | GGTACC | 1 cut(s) | 2644 |
| AspLEI | GCGC | 1 cut(s) | 2610 |
| AspS9I | GGNCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| AsuHPI | GGTGA | 7 cut(s) | 163, 238, 352, 1389, 1429, 1815, 1875 |
| AsuII | TTCGAA | 2 cut(s) | 616, 1558 |
| AvaI | CYCGRG | 1 cut(s) | 2549 |
| AvaII | GGWCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| AxyI | CCTNAGG | 1 cut(s) | 1413 |
| BaeGI | GKGCMC | 1 cut(s) | 1615 |
| BanI | GGYRCC | 3 cut(s) | 1633, 2644, 2725 |
| BbsI | GAAGAC | 1 cut(s) | 2737 |
| BbvCI | CCTCAGC | 1 cut(s) | 1836 |
| BbvI | GCAGC | 7 cut(s) | 635, 890, 1096, 1454, 1582, 1820, 2506 |
| BccI | CCATC | 8 cut(s) | 28, 136, 992, 1160, 1456, 2338, 2489, 2644 |
| BciT130I | CCWGG | 4 cut(s) | 662, 1153, 1638, 2718 |
| BclI | TGATCA | 1 cut(s) | 1144 |
| BcoDI | GTCTC | 4 cut(s) | 547, 718, 2268, 2752 |
| BcuI | ACTAGT | 1 cut(s) | 727 |
| BfaI | CTAG | 7 cut(s) | 395, 728, 923, 1122, 1695, 1860, 2108 |
| BfmI | CTRYAG | 1 cut(s) | 1376 |
| BfuAI | ACCTGC | 1 cut(s) | 2445 |
| BglII | AGATCT | 1 cut(s) | 2357 |
| BlpI | GCTNAGC | 1 cut(s) | 1086 |
| Bme1390I | CCNGG | 4 cut(s) | 662, 1153, 1638, 2718 |
| Bme18I | GGWCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| BmeT110I | CYCGRG | 1 cut(s) | 2549 |
| BmgT120I | GGNCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| BmiI | GGNNCC | 9 cut(s) | 1098, 1635, 1782, 1783, 1842, 2206, 2634, 2646, 2727 |
| BmrFI | CCNGG | 4 cut(s) | 662, 1153, 1638, 2718 |
| BmrI | ACTGGG | 1 cut(s) | 2390 |
| BmsI | GCATC | 6 cut(s) | 261, 556, 1259, 1270, 1953, 2541 |
| BmuI | ACTGGG | 1 cut(s) | 2390 |
| BpiI | GAAGAC | 1 cut(s) | 2737 |
| BpmI | CTGGAG | 2 cut(s) | 2008, 2288 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1836 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1086 |
| Bpu14I | TTCGAA | 2 cut(s) | 616, 1558 |
| BpuEI | CTTGAG | 3 cut(s) | 155, 479, 731 |
| Bsa29I | ATCGAT | 2 cut(s) | 266, 539 |
| BsaAI | YACGTR | 2 cut(s) | 1521, 2213 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1681 |
| BsaI | GGTCTC | 2 cut(s) | 2268, 2752 |
| BsaJI | CCNNGG | 2 cut(s) | 661, 1637 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 215, 235, 245, 265, 347, 377, 1699, 1729 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 790, 874, 916 |
| Bse1I | ACTGG | 5 cut(s) | 420, 702, 1991, 2116, 2396 |
| Bse21I | CCTNAGG | 1 cut(s) | 1413 |
| Bse3DI | GCAATG | 3 cut(s) | 281, 1290, 1764 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1681 |
| BseBI | CCWGG | 4 cut(s) | 662, 1153, 1638, 2718 |
| BseCI | ATCGAT | 2 cut(s) | 266, 539 |
| BseDI | CCNNGG | 2 cut(s) | 661, 1637 |
| BseGI | GGATG | 5 cut(s) | 571, 1171, 1285, 2428, 2500 |
| BseJI | GATNNNNATC | 1 cut(s) | 1681 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 790, 874, 916 |
| BseMI | GCAATG | 3 cut(s) | 281, 1290, 1764 |
| BseMII | CTCAG | 6 cut(s) | 1427, 1595, 1662, 1767, 1827, 2527 |
| BseNI | ACTGG | 5 cut(s) | 420, 702, 1991, 2116, 2396 |
| BseRI | GAGGAG | 7 cut(s) | 143, 212, 236, 317, 371, 644, 1707 |
| BseSI | GKGCMC | 1 cut(s) | 1615 |
| BseXI | GCAGC | 7 cut(s) | 635, 890, 1096, 1454, 1582, 1820, 2506 |
| BshFI | GGCC | 3 cut(s) | 660, 701, 1037 |
| BshNI | GGYRCC | 3 cut(s) | 1633, 2644, 2725 |
| BshVI | ATCGAT | 2 cut(s) | 266, 539 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2549 |
| BslFI | GGGAC | 5 cut(s) | 678, 1654, 1794, 1854, 2073 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 790, 874, 916 |
| BsmAI | GTCTC | 4 cut(s) | 547, 718, 2268, 2752 |
| BsmFI | GGGAC | 5 cut(s) | 678, 1654, 1794, 1854, 2073 |
| BsmI | GAATGC | 1 cut(s) | 2111 |
| BsnI | GGCC | 3 cut(s) | 660, 701, 1037 |
| Bso31I | GGTCTC | 2 cut(s) | 2268, 2752 |
| BsoBI | CYCGRG | 1 cut(s) | 2549 |
| Bsp119I | TTCGAA | 2 cut(s) | 616, 1558 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1615 |
| Bsp143I | GATC | 7 cut(s) | 309, 925, 1144, 1155, 1676, 2357, 2509 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1086 |
| BspACI | CCGC | 5 cut(s) | 10, 121, 1473, 1507, 2260 |
| BspANI | GGCC | 3 cut(s) | 660, 701, 1037 |
| BspCNI | CTCAG | 6 cut(s) | 1426, 1594, 1663, 1768, 1828, 2526 |
| BspDI | ATCGAT | 2 cut(s) | 266, 539 |
| BspHI | TCATGA | 1 cut(s) | 1188 |
| BspLI | GGNNCC | 9 cut(s) | 1098, 1635, 1782, 1783, 1842, 2206, 2634, 2646, 2727 |
| BspMI | ACCTGC | 1 cut(s) | 2445 |
| BspPI | GGATC | 1 cut(s) | 1163 |
| BspQI | GCTCTTC | 1 cut(s) | 687 |
| BspT104I | TTCGAA | 2 cut(s) | 616, 1558 |
| BspT107I | GGYRCC | 3 cut(s) | 1633, 2644, 2725 |
| BspTNI | GGTCTC | 2 cut(s) | 2268, 2752 |
| BsrDI | GCAATG | 3 cut(s) | 281, 1290, 1764 |
| BsrI | ACTGG | 5 cut(s) | 420, 702, 1991, 2116, 2396 |
| BssECI | CCNNGG | 2 cut(s) | 661, 1637 |
| BssMI | GATC | 7 cut(s) | 309, 925, 1144, 1155, 1676, 2357, 2509 |
| Bst2UI | CCWGG | 4 cut(s) | 662, 1153, 1638, 2718 |
| Bst4CI | ACNGT | 4 cut(s) | 919, 1182, 1315, 2288 |
| Bst6I | CTCTTC | 5 cut(s) | 467, 543, 687, 1761, 2658 |
| BstBAI | YACGTR | 2 cut(s) | 1521, 2213 |
| BstBI | TTCGAA | 2 cut(s) | 616, 1558 |
| BstC8I | GCNNGC | 4 cut(s) | 59, 699, 1035, 1692 |
| BstDEI | CTNAG | 8 cut(s) | 1086, 1413, 1581, 1671, 1776, 1836, 2237, 2513 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 788 |
| BstF5I | GGATG | 5 cut(s) | 571, 1171, 1285, 2428, 2500 |
| BstHHI | GCGC | 1 cut(s) | 2610 |
| BstKTI | GATC | 7 cut(s) | 312, 928, 1147, 1158, 1679, 2360, 2512 |
| BstMAI | GTCTC | 4 cut(s) | 547, 718, 2268, 2752 |
| BstMBI | GATC | 7 cut(s) | 309, 925, 1144, 1155, 1676, 2357, 2509 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 67, 293, 892, 1096, 1118, 1472, 1805 |
| BstNI | CCWGG | 4 cut(s) | 662, 1153, 1638, 2718 |
| BstNSI | RCATGY | 1 cut(s) | 2420 |
| BstSCI | CCNGG | 4 cut(s) | 660, 1151, 1636, 2716 |
| BstSFI | CTRYAG | 1 cut(s) | 1376 |
| BstSLI | GKGCMC | 1 cut(s) | 1615 |
| BstSNI | TACGTA | 1 cut(s) | 1521 |
| BstV1I | GCAGC | 7 cut(s) | 635, 890, 1096, 1454, 1582, 1820, 2506 |
| BstV2I | GAAGAC | 1 cut(s) | 2737 |
| BstX2I | RGATCY | 1 cut(s) | 2357 |
| BstYI | RGATCY | 1 cut(s) | 2357 |
| Bsu15I | ATCGAT | 2 cut(s) | 266, 539 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1413 |
| BsuRI | GGCC | 3 cut(s) | 660, 701, 1037 |
| BsuTUI | ATCGAT | 2 cut(s) | 266, 539 |
| BtgZI | GCGATG | 1 cut(s) | 47 |
| BtsCI | GGATG | 5 cut(s) | 571, 1171, 1285, 2428, 2500 |
| BtsI | GCAGTG | 2 cut(s) | 569, 1437 |
| BtsIMutI | CAGTG | 6 cut(s) | 569, 709, 1437, 2123, 2403, 2755 |
| BveI | ACCTGC | 1 cut(s) | 2445 |
| Cac8I | GCNNGC | 4 cut(s) | 59, 699, 1035, 1692 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1940 |
| CciI | TCATGA | 1 cut(s) | 1188 |
| CfoI | GCGC | 1 cut(s) | 2610 |
| Cfr13I | GGNCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| ClaI | ATCGAT | 2 cut(s) | 266, 539 |
| Csp6I | GTAC | 3 cut(s) | 962, 2645, 2688 |
| CviAII | CATG | 3 cut(s) | 1162, 1189, 2417 |
| CviQI | GTAC | 3 cut(s) | 962, 2645, 2688 |
| DdeI | CTNAG | 8 cut(s) | 1086, 1413, 1581, 1671, 1776, 1836, 2237, 2513 |
| DpnI | GATC | 7 cut(s) | 311, 927, 1146, 1157, 1678, 2359, 2511 |
| DpnII | GATC | 7 cut(s) | 309, 925, 1144, 1155, 1676, 2357, 2509 |
| DraI | TTTAAA | 1 cut(s) | 718 |
| Eam1104I | CTCTTC | 5 cut(s) | 467, 543, 687, 1761, 2658 |
| EarI | CTCTTC | 5 cut(s) | 467, 543, 687, 1761, 2658 |
| Eco105I | TACGTA | 1 cut(s) | 1521 |
| Eco31I | GGTCTC | 2 cut(s) | 2268, 2752 |
| Eco47I | GGWCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| Eco57I | CTGAAG | 2 cut(s) | 2166, 2731 |
| Eco81I | CCTNAGG | 1 cut(s) | 1413 |
| Eco88I | CYCGRG | 1 cut(s) | 2549 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 788 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1781 |
| EcoRII | CCWGG | 4 cut(s) | 660, 1151, 1636, 2716 |
| EcoT22I | ATGCAT | 1 cut(s) | 2534 |
| FaeI | CATG | 3 cut(s) | 1165, 1192, 2420 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1695, 1727 |
| FaqI | GGGAC | 5 cut(s) | 678, 1654, 1794, 1854, 2073 |
| FatI | CATG | 3 cut(s) | 1161, 1188, 2416 |
| FbaI | TGATCA | 1 cut(s) | 1144 |
| FokI | GGATG | 5 cut(s) | 578, 1178, 1292, 2435, 2507 |
| FspBI | CTAG | 7 cut(s) | 395, 728, 923, 1122, 1695, 1860, 2108 |
| FspI | TGCGCA | 1 cut(s) | 2609 |
| GlaI | GCGC | 1 cut(s) | 2609 |
| GsuI | CTGGAG | 2 cut(s) | 2008, 2288 |
| HaeIII | GGCC | 3 cut(s) | 660, 701, 1037 |
| HhaI | GCGC | 1 cut(s) | 2610 |
| Hin1II | CATG | 3 cut(s) | 1165, 1192, 2420 |
| Hin6I | GCGC | 1 cut(s) | 2608 |
| HinP1I | GCGC | 1 cut(s) | 2608 |
| HindIII | AAGCTT | 2 cut(s) | 1174, 2321 |
| HphI | GGTGA | 7 cut(s) | 163, 238, 352, 1389, 1429, 1815, 1875 |
| Hpy188III | TCNNGA | 6 cut(s) | 929, 1189, 1666, 2267, 2492, 2757 |
| HpyCH4III | ACNGT | 4 cut(s) | 919, 1182, 1315, 2288 |
| HpyCH4IV | ACGT | 4 cut(s) | 1520, 1979, 2073, 2212 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 67, 293, 892, 1096, 1118, 1472, 1805 |
| HpyF3I | CTNAG | 8 cut(s) | 1086, 1413, 1581, 1671, 1776, 1836, 2237, 2513 |
| HpySE526I | ACGT | 4 cut(s) | 1520, 1979, 2073, 2212 |
| Hsp92II | CATG | 3 cut(s) | 1165, 1192, 2420 |
| HspAI | GCGC | 1 cut(s) | 2608 |
| KflI | GGGWCCC | 1 cut(s) | 1781 |
| KpnI | GGTACC | 1 cut(s) | 2648 |
| Ksp22I | TGATCA | 1 cut(s) | 1144 |
| Kzo9I | GATC | 7 cut(s) | 309, 925, 1144, 1155, 1676, 2357, 2509 |
| LguI | GCTCTTC | 1 cut(s) | 687 |
| LmnI | GCTCC | 6 cut(s) | 130, 391, 631, 1096, 1552, 2394 |
| Lsp1109I | GCAGC | 7 cut(s) | 635, 890, 1096, 1454, 1582, 1820, 2506 |
| LweI | GCATC | 6 cut(s) | 261, 556, 1259, 1270, 1953, 2541 |
| MaeI | CTAG | 7 cut(s) | 395, 728, 923, 1122, 1695, 1860, 2108 |
| MaeII | ACGT | 4 cut(s) | 1520, 1979, 2073, 2212 |
| MaeIII | GTNAC | 5 cut(s) | 1395, 1468, 1915, 2457, 2709 |
| MalI | GATC | 7 cut(s) | 311, 927, 1146, 1157, 1678, 2359, 2511 |
| MboI | GATC | 7 cut(s) | 309, 925, 1144, 1155, 1676, 2357, 2509 |
| MflI | RGATCY | 1 cut(s) | 2357 |
| MhlI | GDGCHC | 1 cut(s) | 1615 |
| MlyI | GAGTC | 4 cut(s) | 587, 644, 1253, 2561 |
| MmeI | TCCRAC | 5 cut(s) | 270, 543, 963, 1935, 2056 |
| Mph1103I | ATGCAT | 1 cut(s) | 2534 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2245 |
| MslI | CAYNNNNRTG | 2 cut(s) | 2116, 2421 |
| MspA1I | CMGCKG | 4 cut(s) | 61, 1238, 1445, 1475 |
| MspR9I | CCNGG | 4 cut(s) | 662, 1153, 1638, 2718 |
| Mva1269I | GAATGC | 1 cut(s) | 2111 |
| MvaI | CCWGG | 4 cut(s) | 662, 1153, 1638, 2718 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 67, 293, 892, 1096, 1118, 1472, 1805 |
| NdeII | GATC | 7 cut(s) | 309, 925, 1144, 1155, 1676, 2357, 2509 |
| NlaIII | CATG | 3 cut(s) | 1165, 1192, 2420 |
| NlaIV | GGNNCC | 9 cut(s) | 1098, 1635, 1782, 1783, 1842, 2206, 2634, 2646, 2727 |
| NmeAIII | GCCGAG | 1 cut(s) | 1241 |
| NmuCI | GTSAC | 3 cut(s) | 1395, 1915, 2457 |
| NsbI | TGCGCA | 1 cut(s) | 2609 |
| NsiI | ATGCAT | 1 cut(s) | 2534 |
| NspI | RCATGY | 1 cut(s) | 2420 |
| NspV | TTCGAA | 2 cut(s) | 616, 1558 |
| PaeR7I | CTCGAG | 1 cut(s) | 2549 |
| PagI | TCATGA | 1 cut(s) | 1188 |
| PciI | ACATGT | 1 cut(s) | 2416 |
| PciSI | GCTCTTC | 1 cut(s) | 687 |
| PctI | GAATGC | 1 cut(s) | 2111 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2245 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 874 |
| PfoI | TCCNGGA | 1 cut(s) | 1151 |
| PleI | GAGTC | 4 cut(s) | 586, 644, 1253, 2560 |
| PpsI | GAGTC | 4 cut(s) | 586, 644, 1253, 2560 |
| Ppu21I | YACGTR | 2 cut(s) | 1521, 2213 |
| PpuMI | RGGWCCY | 1 cut(s) | 1781 |
| PscI | ACATGT | 1 cut(s) | 2416 |
| PshBI | ATTAAT | 1 cut(s) | 1407 |
| Psp5II | RGGWCCY | 1 cut(s) | 1781 |
| Psp6I | CCWGG | 4 cut(s) | 660, 1151, 1636, 2716 |
| PspGI | CCWGG | 4 cut(s) | 660, 1151, 1636, 2716 |
| PspN4I | GGNNCC | 9 cut(s) | 1098, 1635, 1782, 1783, 1842, 2206, 2634, 2646, 2727 |
| PspPI | GGNCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| PspPPI | RGGWCCY | 1 cut(s) | 1781 |
| PspXI | VCTCGAGB | 1 cut(s) | 2549 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1940 |
| PsuI | RGATCY | 1 cut(s) | 2357 |
| PvuII | CAGCTG | 3 cut(s) | 61, 1238, 1445 |
| RsaI | GTAC | 3 cut(s) | 963, 2646, 2689 |
| RsaNI | GTAC | 3 cut(s) | 962, 2645, 2688 |
| RseI | CAYNNNNRTG | 2 cut(s) | 2116, 2421 |
| SapI | GCTCTTC | 1 cut(s) | 687 |
| Sau3AI | GATC | 7 cut(s) | 309, 925, 1144, 1155, 1676, 2357, 2509 |
| Sau96I | GGNCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| SchI | GAGTC | 4 cut(s) | 587, 644, 1253, 2561 |
| ScrFI | CCNGG | 4 cut(s) | 662, 1153, 1638, 2718 |
| SduI | GDGCHC | 1 cut(s) | 1615 |
| SfaNI | GCATC | 6 cut(s) | 261, 556, 1259, 1270, 1953, 2541 |
| SfcI | CTRYAG | 1 cut(s) | 1376 |
| Sfr274I | CTCGAG | 1 cut(s) | 2549 |
| SfuI | TTCGAA | 2 cut(s) | 616, 1558 |
| SinI | GGWCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| SlaI | CTCGAG | 1 cut(s) | 2549 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 2116, 2421 |
| SmlI | CTYRAG | 4 cut(s) | 134, 458, 710, 2549 |
| SmoI | CTYRAG | 4 cut(s) | 134, 458, 710, 2549 |
| SnaBI | TACGTA | 1 cut(s) | 1521 |
| SpeI | ACTAGT | 1 cut(s) | 727 |
| SsiI | CCGC | 5 cut(s) | 10, 121, 1473, 1507, 2260 |
| SspI | AATATT | 2 cut(s) | 1730, 2035 |
| SspMI | CTAG | 7 cut(s) | 395, 728, 923, 1122, 1695, 1860, 2108 |
| StyD4I | CCNGG | 4 cut(s) | 660, 1151, 1636, 2716 |
| TaaI | ACNGT | 4 cut(s) | 919, 1182, 1315, 2288 |
| TaiI | ACGT | 4 cut(s) | 1523, 1982, 2076, 2215 |
| TaqI | TCGA | 8 cut(s) | 266, 539, 616, 690, 1558, 1961, 2550, 2756 |
| TaqII | GACCGA | 1 cut(s) | 1290 |
| TauI | GCSGC | 2 cut(s) | 13, 1510 |
| TscAI | CASTG | 6 cut(s) | 576, 709, 1437, 2123, 2403, 2755 |
| TseFI | GTSAC | 3 cut(s) | 1395, 1915, 2457 |
| TseI | GCWGC | 8 cut(s) | 7, 623, 878, 1109, 1442, 1595, 1808, 2519 |
| Tsp45I | GTSAC | 3 cut(s) | 1395, 1915, 2457 |
| TspRI | CASTG | 6 cut(s) | 576, 709, 1437, 2123, 2403, 2755 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 874 |
| VpaK11BI | GGWCC | 4 cut(s) | 865, 1781, 1841, 2078 |
| VspI | ATTAAT | 1 cut(s) | 1407 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 788 |
| XapI | RAATTY | 3 cut(s) | 601, 808, 2738 |
| XceI | RCATGY | 1 cut(s) | 2420 |
| XhoI | CTCGAG | 1 cut(s) | 2549 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2245 |
| XspI | CTAG | 7 cut(s) | 395, 728, 923, 1122, 1695, 1860, 2108 |
| Zsp2I | ATGCAT | 1 cut(s) | 2534 |