Rh7BG220200

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000126
Physical Location & Seq
Forward (+)
18925797 .. 18929605
3809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG220200.1

Sequence Viewer

Length: 2490 bp
ATGGTGAACAAGTGCAGTGAGGATATGGAATTGGAAGAGAAGAGCAAGAAAGAGTGTGGTTTGGACCAGAACTCATTTGAGCAATGTGAGAAAGAGATGGAGTTGAAAAGAGGGGAGCTTGATTCGAATCGGAAATCGATGGCGGAATGTTCTGATACAGTTGAATTGAGAGGGGGAAAGATGAGGGAAATGTGTGGAGGTTTTAGTTTGCTTAAGAAGTCGATGGAGGAGTGGTGCTGTAAGGTTGAATTGAAAGAGAGGGGGATTGAGGAGTGTGTTGCCAAGCTTGAATTGAAGGAGAAACAAGTTGAATCGAGATTTGAGGAGCTTAATGTGATTGATAAGAGGGTTAATGAGTGCCTCCGTGATGCTCAGTTGAAAGAAAAGCAGTTGGATTCTGTTATGAAATTAATACAAGAAGACAAACAGCATTTGGATTGGCTCCAAAAGTCGGTGGAAGAGCATGAAAAACAGTTGGGTTTGGTATCAAATGGACTTCAAGGAAAGGCGAGGGAACTTGAAGTGCAACAACAACAAATTGATTCAGTCTACCATGCGTTACAGATGAAAGAGAATGAACTTGAACAGCAGGCCCAAGAGCTTGATTTGAAACGCCAGCAATATGAGTCACTTTGCCATCTGTTTCAGATCAAAGAGGGTCAACTTGAAAAGCAATCCAAAGATCTTGAATTGAAACACAAACAAATTGAATCCATCCACCATCTGATTCGAATTAAAGAGTCGCAACTTGAAAATCGAGCCAAGGAGCTTGGATTCAAACAGCAACAAAATGATTCAATCTCTCAACTACTTAAGATGAAAGAAAGGGAACTTGAACAGCAGGAAAAAGAGCTTGAACCCAAACAACAGCAAATTGTTTCGGTTTCGCAGACACTTCAGATGAAAGAGAGGAAACTTGAACAACAGGCAAAAGAGCTTGACTTGAAGCAAAAACAATATGATTCTCAGGTGAAGATGGTCCAGTTGATAGCTGCACCTGCTGCTAACAATGCCACTATTCCTTCATCTGCAACTGATGAATCCAGGTCCAGCATCAATATAGATGGTCGAGGCTTGCAGTTTTTTATGTATGAACAATTGAAGAAAATTGATTCAATGCGTGCCCAAATGTTGGCTGCTTTTCAAGTGTCATCAGACCCAGGAAATTTGGTTTTGGATGCAATGCAAGGATTTTACCCTTCAAATTCAAATATGGGAAAGAGGGAGACTGATTTTGATCTGAGCTTTATTAGAAGAAGTTGTATTCTTCTGTTACAGGAGTTAAAGAGGATGTCACCACAACTTAATCCTGGGGTAAGAGAAAAAGCATTGAAGTTGGCAGCTCATTGGAAGGCCAAGTTGACAGTGGCCACTGATAATTCGTTGGAGGTTTTGGGATTTTTGCGGCTTGTAACTGCGTTTGAATTAACCTCTGCTTATGATGCCAATGAGCTTCAGAGTCTTCTTTCTGTAGTTACTCAGCCCGAACAAGCAGCTCAATTAAGAAAGGCTCTCGGTGTCAAAGATAAGGAACCTTCAACCAACAGCATTTCTCCCCTAGTCAAAGTGGTGGAACCAGCATCTACCAATGCAGCAACCTTTCCTTCTTCAAATATTCAACCAAATGTCACTAAGGATGCAGGGAATGTGTCGGGGTTTGGAAATAAGCCTTTAAGTGGGAATCATTCTATACTGGCTGCTTCTGTTCCATTGTCATCAGACCCAGCTAAACTCGTGTTGGATAGCATCCAAAAATCTCTTGCTGAATACTTGCGAAATGGAGATTGTGAAGAAAGTATCATGTCAAGTAACATTCGCCTATTGGAGGAGCTAATGAGAGTTTCACCAAATGTTGGACCTAATCTGAAAGCAGAAGCAACAAATCTATCAGTCCAGTGGAAAGCAAAGATGGCAGGAAATACTGAGAAGTCATTGGAGGGTGTAGGATTTTTGCTATTTTTAGCTACATATGGATTGCTTTCTACGTTAAATGCTGATGAGATTGTAAAGCATCTTGGGTTGATTTGTCACCATAAACAGGCTCTAGAATTATGCCAGGCATGTGGTTTTGCAGATAAGATTGGTGAATTTATTCGGATCCTTGTTGAAAAGAAGCAACTTATTGAGGCTGTTAGATTTATTTTTGCGTTCAAGTTAACTGACAAGCTTTCCCCAGTCCAACTTTTAAAAGAATATTTGGAGGATACAAAAAAGTGTTCTGAAGCAATTTTCATGTTGCAGATATCACAAGATGAAAAGGAGAAGGTGGCAGATGGTCTGTCAGGTGATCTAAGAGCTGTGCATCAATACATTAAGGATTACAATCTTGAGTCCAAATACCCATCTGCAGACATTGAAATGCAAATAGTTAAGCTGGAAACAGTAAAGTCTTCCCCTGCCTCCAATGTCGAACAGCAAGAGCAGAGAAAAAGGAAGAAACCTAATGCTGGCACTTCGACCCTTGAGATCCAACCACCACAACAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida

Protein Analysis

829

Amino Acids

93.78

Weight (kDa)

5.81

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 353 - 510 6.9e-36 Frigida-like protein
Frigida PF07899 571 - 801 1.2e-50 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1006
Acc36I ACCTGC 1 cut(s) 1006
AccB7I CCANNNNNTGG 2 cut(s) 1132, 1853
AccI GTMKAC 1 cut(s) 549
AciI CCGC 2 cut(s) 143, 1405
AclWI GGATC 3 cut(s) 2092, 2105, 2461
AcoI YGGCCR 1 cut(s) 1368
AcsI RAATTY 3 cut(s) 1165, 1204, 2087
AcuI CTGAAG 3 cut(s) 881, 1439, 2241
AfiI CCNNNNNNNGG 7 cut(s) 451, 1132, 1676, 1825, 1853, 2038, 2447
AflII CTTAAG 2 cut(s) 212, 812
AjnI CCWGG 4 cut(s) 1043, 1159, 1309, 2055
Alw26I GTCTC 1 cut(s) 1220
AlwI GGATC 3 cut(s) 2092, 2105, 2461
AoxI GGCC 4 cut(s) 591, 1353, 1368, 2484
ApeKI GCWGC 7 cut(s) 992, 1001, 1136, 1340, 1493, 1592, 1697
ApoI RAATTY 3 cut(s) 1165, 1204, 2087
AseI ATTAAT 1 cut(s) 410
Asp700I GAANNNNTTC 1 cut(s) 2091
AspS9I GGNCC 5 cut(s) 64, 592, 979, 1047, 1856
AsuHPI GGTGA 7 cut(s) 16, 982, 1287, 1836, 2021, 2096, 2297
AsuII TTCGAA 2 cut(s) 125, 730
AvaII GGWCC 4 cut(s) 64, 979, 1047, 1856
BaeGI GKGCMC 1 cut(s) 1126
BalI TGGCCA 1 cut(s) 1370
BamHI GGATCC 1 cut(s) 2097
BauI CACGAG 1 cut(s) 1733
BbsI GAAGAC 3 cut(s) 426, 1454, 2382
BbvI GCAGC 7 cut(s) 979, 988, 1123, 1352, 1505, 1604, 1684
BciT130I CCWGG 4 cut(s) 1045, 1161, 1311, 2057
BciVI GTATCC 1 cut(s) 2197
BcoDI GTCTC 1 cut(s) 1220
BfaI CTAG 2 cut(s) 1559, 2045
BfmI CTRYAG 2 cut(s) 1470, 2346
BfrI CTTAAG 2 cut(s) 212, 812
BfuAI ACCTGC 1 cut(s) 1006
BfuI GTATCC 1 cut(s) 2197
BglII AGATCT 1 cut(s) 682
BisI GCNGC 8 cut(s) 993, 1002, 1137, 1341, 1406, 1494, 1593, 1698
BlsI GCNGC 8 cut(s) 994, 1003, 1138, 1342, 1407, 1495, 1594, 1699
Bme1390I CCNGG 4 cut(s) 1045, 1161, 1311, 2057
Bme18I GGWCC 4 cut(s) 64, 979, 1047, 1856
BmgT120I GGNCC 5 cut(s) 64, 592, 979, 1047, 1856
BmiI GGNNCC 4 cut(s) 443, 1533, 1575, 2099
BmrFI CCNGG 4 cut(s) 1045, 1161, 1311, 2057
BmrI ACTGGG 1 cut(s) 2168
BmsI GCATC 9 cut(s) 358, 1062, 1168, 1432, 1589, 1627, 1755, 2020, 2311
BmuI ACTGGG 1 cut(s) 2168
BpiI GAAGAC 3 cut(s) 426, 1454, 2382
Bpu14I TTCGAA 2 cut(s) 125, 730
BpuEI CTTGAG 2 cut(s) 2348, 2483
Bsa29I ATCGAT 1 cut(s) 137
BsaBI GATNNNNATC 3 cut(s) 126, 1236, 2322
BsaJI CCNNGG 3 cut(s) 762, 1159, 1310
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 7 cut(s) 451, 1132, 1676, 1825, 1853, 2038, 2447
Bse1I ACTGG 4 cut(s) 982, 1698, 1894, 2174
Bse3DI GCAATG 2 cut(s) 89, 1188
Bse8I GATNNNNATC 3 cut(s) 126, 1236, 2322
BseBI CCWGG 4 cut(s) 1045, 1161, 1311, 2057
BseCI ATCGAT 1 cut(s) 137
BseDI CCNNGG 3 cut(s) 762, 1159, 1310
BseGI GGATG 5 cut(s) 714, 1183, 1296, 1642, 1746
BseJI GATNNNNATC 3 cut(s) 126, 1236, 2322
BseLI CCNNNNNNNGG 7 cut(s) 451, 1132, 1676, 1825, 1853, 2038, 2447
BseMI GCAATG 2 cut(s) 89, 1188
BseMII CTCAG 5 cut(s) 386, 980, 1232, 1493, 1914
BseNI ACTGG 4 cut(s) 982, 1698, 1894, 2174
BseRI GAGGAG 4 cut(s) 242, 284, 338, 1841
BseSI GKGCMC 1 cut(s) 1126
BseXI GCAGC 7 cut(s) 979, 988, 1123, 1352, 1505, 1604, 1684
BseYI CCCAGC 1 cut(s) 1723
BsgI GTGCAG 2 cut(s) 34, 978
BshFI GGCC 4 cut(s) 593, 1355, 1370, 2486
BshVI ATCGAT 1 cut(s) 137
BslI CCNNNNNNNGG 7 cut(s) 451, 1132, 1676, 1825, 1853, 2038, 2447
BsmAI GTCTC 1 cut(s) 1220
BsnI GGCC 4 cut(s) 593, 1355, 1370, 2486
Bsp119I TTCGAA 2 cut(s) 125, 730
Bsp1286I GDGCHC 1 cut(s) 1126
Bsp143I GATC 6 cut(s) 648, 682, 1237, 2097, 2287, 2466
BspACI CCGC 2 cut(s) 143, 1405
BspANI GGCC 4 cut(s) 593, 1355, 1370, 2486
BspCNI CTCAG 5 cut(s) 385, 979, 1233, 1492, 1915
BspDI ATCGAT 1 cut(s) 137
BspLI GGNNCC 4 cut(s) 443, 1533, 1575, 2099
BspMAI CTGCAG 1 cut(s) 2350
BspMI ACCTGC 1 cut(s) 1006
BspPI GGATC 3 cut(s) 2092, 2105, 2461
BspQI GCTCTTC 2 cut(s) 35, 453
BspT104I TTCGAA 2 cut(s) 125, 730
BspTI CTTAAG 2 cut(s) 212, 812
BsrDI GCAATG 2 cut(s) 89, 1188
BsrI ACTGG 4 cut(s) 982, 1698, 1894, 2174
BssECI CCNNGG 3 cut(s) 762, 1159, 1310
BssMI GATC 6 cut(s) 648, 682, 1237, 2097, 2287, 2466
BssSI CACGAG 1 cut(s) 1733
BssT1I CCWWGG 1 cut(s) 762
Bst2BI CACGAG 1 cut(s) 1733
Bst2UI CCWGG 4 cut(s) 1045, 1161, 1311, 2057
Bst4CI ACNGT 4 cut(s) 160, 474, 1366, 2383
Bst6I CTCTTC 3 cut(s) 30, 35, 453
BstAFI CTTAAG 2 cut(s) 212, 812
BstAPI GCANNNNNTGC 1 cut(s) 1001
BstBI TTCGAA 2 cut(s) 125, 730
BstC8I GCNNGC 5 cut(s) 591, 617, 1076, 1122, 2449
BstDEI CTNAG 7 cut(s) 372, 966, 1241, 1479, 1632, 1923, 2291
BstENI CCTNNNNNAGG 1 cut(s) 1823
BstF5I GGATG 5 cut(s) 714, 1183, 1296, 1642, 1746
BstKTI GATC 6 cut(s) 651, 685, 1240, 2100, 2290, 2469
BstMAI GTCTC 1 cut(s) 1220
BstMBI GATC 6 cut(s) 648, 682, 1237, 2097, 2287, 2466
BstMWI GCNNNNNNNGC 5 cut(s) 998, 1001, 1010, 1442, 1910
BstNI CCWGG 4 cut(s) 1045, 1161, 1311, 2057
BstNSI RCATGY 1 cut(s) 2064
BstSCI CCNGG 4 cut(s) 1043, 1159, 1309, 2055
BstSFI CTRYAG 2 cut(s) 1470, 2346
BstSLI GKGCMC 1 cut(s) 1126
BstV1I GCAGC 7 cut(s) 979, 988, 1123, 1352, 1505, 1604, 1684
BstV2I GAAGAC 3 cut(s) 426, 1454, 2382
BstX2I RGATCY 3 cut(s) 682, 2097, 2466
BstXI CCANNNNNNTGG 1 cut(s) 2063
BstYI RGATCY 3 cut(s) 682, 2097, 2466
Bsu15I ATCGAT 1 cut(s) 137
BsuI GTATCC 1 cut(s) 2197
BsuRI GGCC 4 cut(s) 593, 1355, 1370, 2486
BsuTUI ATCGAT 1 cut(s) 137
BtsCI GGATG 5 cut(s) 714, 1183, 1296, 1642, 1746
BtsI GCAGTG 1 cut(s) 22
BtsIMutI CAGTG 4 cut(s) 22, 1371, 1371, 1901
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 5 cut(s) 591, 617, 1076, 1122, 2449
Cfr13I GGNCC 5 cut(s) 64, 592, 979, 1047, 1856
ClaI ATCGAT 1 cut(s) 137
CviAII CATG 5 cut(s) 464, 554, 1801, 2061, 2233
DdeI CTNAG 7 cut(s) 372, 966, 1241, 1479, 1632, 1923, 2291
DpnI GATC 6 cut(s) 650, 684, 1239, 2099, 2289, 2468
DpnII GATC 6 cut(s) 648, 682, 1237, 2097, 2287, 2466
DraI TTTAAA 1 cut(s) 2187
EaeI YGGCCR 1 cut(s) 1368
Eam1104I CTCTTC 3 cut(s) 30, 35, 453
EarI CTCTTC 3 cut(s) 30, 35, 453
EciI GGCGGA 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 762
Eco147I AGGCCT 1 cut(s) 2486
Eco32I GATATC 1 cut(s) 2244
Eco47I GGWCC 4 cut(s) 64, 979, 1047, 1856
Eco57I CTGAAG 3 cut(s) 881, 1439, 2241
EcoNI CCTNNNNNAGG 1 cut(s) 1823
EcoRII CCWGG 4 cut(s) 1043, 1159, 1309, 2055
EcoRV GATATC 1 cut(s) 2244
EcoT14I CCWWGG 1 cut(s) 762
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 5 cut(s) 467, 557, 1804, 2064, 2236
FatI CATG 5 cut(s) 463, 553, 1800, 2060, 2232
FauNDI CATATG 1 cut(s) 1969
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 8 cut(s) 993, 1002, 1137, 1341, 1406, 1494, 1593, 1698
FokI GGATG 5 cut(s) 701, 1190, 1303, 1649, 1733
Fsp4HI GCNGC 8 cut(s) 993, 1002, 1137, 1341, 1406, 1494, 1593, 1698
FspBI CTAG 2 cut(s) 1559, 2045
GluI GCNGC 8 cut(s) 993, 1002, 1137, 1341, 1406, 1494, 1593, 1698
GsaI CCCAGC 1 cut(s) 1727
HaeIII GGCC 4 cut(s) 593, 1355, 1370, 2486
Hin1II CATG 5 cut(s) 467, 557, 1804, 2064, 2236
HincII GTYRAC 3 cut(s) 662, 1362, 2157
HindII GTYRAC 3 cut(s) 662, 1362, 2157
HindIII AAGCTT 2 cut(s) 284, 2165
HpaI GTTAAC 1 cut(s) 2157
HphI GGTGA 7 cut(s) 16, 982, 1287, 1836, 2021, 2096, 2297
Hpy166II GTNNAC 5 cut(s) 7, 550, 662, 1362, 2157
Hpy188III TCNNGA 4 cut(s) 315, 686, 2045, 2327
Hpy8I GTNNAC 5 cut(s) 7, 550, 662, 1362, 2157
HpyAV CCTTC 7 cut(s) 289, 1032, 1209, 1345, 1545, 1614, 2257
HpyCH4III ACNGT 4 cut(s) 160, 474, 1366, 2383
HpyCH4IV ACGT 1 cut(s) 1985
HpyF10VI GCNNNNNNNGC 5 cut(s) 998, 1001, 1010, 1442, 1910
HpyF3I CTNAG 7 cut(s) 372, 966, 1241, 1479, 1632, 1923, 2291
HpySE526I ACGT 1 cut(s) 1985
Hsp92II CATG 5 cut(s) 467, 557, 1804, 2064, 2236
KspAI GTTAAC 1 cut(s) 2157
Kzo9I GATC 6 cut(s) 648, 682, 1237, 2097, 2287, 2466
LguI GCTCTTC 2 cut(s) 35, 453
LmnI GCTCC 5 cut(s) 115, 325, 447, 766, 1828
Lsp1109I GCAGC 7 cut(s) 979, 988, 1123, 1352, 1505, 1604, 1684
LweI GCATC 9 cut(s) 358, 1062, 1168, 1432, 1589, 1627, 1755, 2020, 2311
MaeI CTAG 2 cut(s) 1559, 2045
MaeII ACGT 1 cut(s) 1985
MaeIII GTNAC 9 cut(s) 558, 627, 1272, 1293, 1411, 1474, 1627, 1808, 2027
MalI GATC 6 cut(s) 650, 684, 1239, 2099, 2289, 2468
MboI GATC 6 cut(s) 648, 682, 1237, 2097, 2287, 2466
MfeI CAATTG 1 cut(s) 1097
MflI RGATCY 3 cut(s) 682, 2097, 2466
MhlI GDGCHC 1 cut(s) 1126
MlsI TGGCCA 1 cut(s) 1370
MluNI TGGCCA 1 cut(s) 1370
MlyI GAGTC 4 cut(s) 635, 749, 1468, 2339
MmeI TCCRAC 5 cut(s) 372, 1365, 1719, 1834, 2203
Mox20I TGGCCA 1 cut(s) 1370
MroXI GAANNNNTTC 1 cut(s) 2091
MscI TGGCCA 1 cut(s) 1370
MslI CAYNNNNRTG 1 cut(s) 2357
Msp20I TGGCCA 1 cut(s) 1370
MspCI CTTAAG 2 cut(s) 212, 812
MspR9I CCNGG 4 cut(s) 1045, 1161, 1311, 2057
MunI CAATTG 1 cut(s) 1097
MvaI CCWGG 4 cut(s) 1045, 1161, 1311, 2057
MwoI GCNNNNNNNGC 5 cut(s) 998, 1001, 1010, 1442, 1910
NdeI CATATG 1 cut(s) 1969
NdeII GATC 6 cut(s) 648, 682, 1237, 2097, 2287, 2466
NlaIII CATG 5 cut(s) 467, 557, 1804, 2064, 2236
NlaIV GGNNCC 4 cut(s) 443, 1533, 1575, 2099
NmuCI GTSAC 4 cut(s) 627, 1293, 1627, 2027
NspI RCATGY 1 cut(s) 2064
NspV TTCGAA 2 cut(s) 125, 730
PaqCI CACCTGC 1 cut(s) 1006
PceI AGGCCT 1 cut(s) 2486
PciSI GCTCTTC 2 cut(s) 35, 453
PdmI GAANNNNTTC 1 cut(s) 2091
PflMI CCANNNNNTGG 2 cut(s) 1132, 1853
PkrI GCNGC 8 cut(s) 994, 1003, 1138, 1342, 1407, 1495, 1594, 1699
PleI GAGTC 4 cut(s) 634, 748, 1467, 2338
PpsI GAGTC 4 cut(s) 634, 748, 1467, 2338
PshBI ATTAAT 1 cut(s) 410
Psp6I CCWGG 4 cut(s) 1043, 1159, 1309, 2055
PspFI CCCAGC 1 cut(s) 1723
PspGI CCWGG 4 cut(s) 1043, 1159, 1309, 2055
PspN4I GGNNCC 4 cut(s) 443, 1533, 1575, 2099
PspPI GGNCC 5 cut(s) 64, 592, 979, 1047, 1856
PstI CTGCAG 1 cut(s) 2350
PsuI RGATCY 3 cut(s) 682, 2097, 2466
RseI CAYNNNNRTG 1 cut(s) 2357
SapI GCTCTTC 2 cut(s) 35, 453
SatI GCNGC 8 cut(s) 993, 1002, 1137, 1341, 1406, 1494, 1593, 1698
Sau3AI GATC 6 cut(s) 648, 682, 1237, 2097, 2287, 2466
Sau96I GGNCC 5 cut(s) 64, 592, 979, 1047, 1856
SchI GAGTC 4 cut(s) 635, 749, 1468, 2339
ScrFI CCNGG 4 cut(s) 1045, 1161, 1311, 2057
SduI GDGCHC 1 cut(s) 1126
SfaNI GCATC 9 cut(s) 358, 1062, 1168, 1432, 1589, 1627, 1755, 2020, 2311
SfcI CTRYAG 2 cut(s) 1470, 2346
SfuI TTCGAA 2 cut(s) 125, 730
SinI GGWCC 4 cut(s) 64, 979, 1047, 1856
SmiMI CAYNNNNRTG 1 cut(s) 2357
SmlI CTYRAG 4 cut(s) 212, 812, 2327, 2462
SmoI CTYRAG 4 cut(s) 212, 812, 2327, 2462
SseBI AGGCCT 1 cut(s) 2486
SsiI CCGC 2 cut(s) 143, 1405
SspI AATATT 2 cut(s) 1615, 2195
SspMI CTAG 2 cut(s) 1559, 2045
StuI AGGCCT 1 cut(s) 2486
StyD4I CCNGG 4 cut(s) 1043, 1159, 1309, 2055
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 4 cut(s) 160, 474, 1366, 2383
TaiI ACGT 1 cut(s) 1988
TaqI TCGA 9 cut(s) 125, 137, 221, 314, 730, 757, 1069, 2409, 2456
TauI GCSGC 1 cut(s) 1408
TscAI CASTG 4 cut(s) 22, 1371, 1378, 1901
TseFI GTSAC 4 cut(s) 627, 1293, 1627, 2027
TseI GCWGC 7 cut(s) 992, 1001, 1136, 1340, 1493, 1592, 1697
Tsp45I GTSAC 4 cut(s) 627, 1293, 1627, 2027
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 4 cut(s) 22, 1371, 1378, 1901
Van91I CCANNNNNTGG 2 cut(s) 1132, 1853
Vha464I CTTAAG 2 cut(s) 212, 812
VpaK11BI GGWCC 4 cut(s) 64, 979, 1047, 1856
VspI ATTAAT 1 cut(s) 410
XagI CCTNNNNNAGG 1 cut(s) 1823
XapI RAATTY 3 cut(s) 1165, 1204, 2087
XbaI TCTAGA 1 cut(s) 2044
XceI RCATGY 1 cut(s) 2064
XcmI CCANNNNNNNNNTGG 1 cut(s) 1363
XmiI GTMKAC 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 2091
XspI CTAG 2 cut(s) 1559, 2045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.