Rw3G003710

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000126
Physical Location & Seq
Forward (+)
3179719 .. 3184567
4849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003710.1

Sequence Viewer

Length: 2853 bp
ATGGAGACTGCGGCGGCGGAGTTGGAACAAAAATCGGAGGCGAAACAGAGGAGCCTAGGCGCGGCGTTTGAGTCGTTGAGTTCGCAAGCTTCGTCGATTCTGAAGTTTACGGTGGAGTGGAAGGAGCTGGAGGACCATTTCGAGTCGACCCGGAGGGTGCTCCGGACCCGGTTCGAGGAGGTAAGGGATCGCGTGAAGGAGGTGGCGGAGATGGAGGCGAAGGAGGAGAGGCTGAAGATGGAGGTGAAGGGGAGGGAGAAGGAGTTGGGGGAAATCGAGGAGGTGGTGGAGCGGAGGAGGAGGGAGGTGGAGGAAAGTGAGAGTTATTTGGAGTCTGTGAGGGCTTTGATTGTGGAGAATGATGAGGAGCTTAGGGTTAGGGAGGAGAGGTATGATAAAGTTGAGAGATTGATTAGGGAGAAGGAGAGGGAGGTGGAGGAGATGGAGAAGCATGTGAGAGAGAGGTCTAGGAAGTTGAGTTGGCTGGACAAGAGGATAGAGGTGAAGAAGAAGGAGGTTGAGAGGAAGGAGAGGGAGCTGAGAGAGGTTAGGGATTTGGAGGAGAAGAAATTGAGTGGTGTTAGGGGGTTGATTGAGGAGAAGAGGAAGGAAGTTGAGATTGTGAGGGAAATGAAGGAGAAAGAGGAGAGGCTGAGTTTGATTGAGAAATCGAAAGAGGAAATGATTGGTGTGGTGGATTTGAAAGGGAAAGAGTTCTGTTTGCTGAAGAAATCAATGGAGGAGTGGTGCTGTAAGATTGAAGTGAAGGAGAGGGAGCTTGAAGGATGGGTTGATAAGTTGGAATCGAAAGAGAAGGAAGTTGAATTGAAGGTTGAGGAGCTTGATTTGATTCGTAGCAAGTTCCTTGATGAGGTTCAGTTCAAAGAAAAGCATTTGGATTCGCTTGAAAAATCGTTACTAGAATGGGAGGAGGATCTGCATTCCCTCCAGAAGTCGGTACAAGAATGTTCCCGGGGAAGTGAGATCATTAGGGATGGAAGAGGATTGCAGCAGTTCATGGACGAGCATCTGCAGAGAATTGATTCCATGGGTACTGAACTGTCAGCGATTCTTAAAGACTCTTCAGACCCCGGGAAGTTGGTTTTGGATGCAATGCAGGGGTTTTATGCTGACAACAGGGAGTTAGATTTTGAATTGAGAGAGAATCTCCCACAAATGAATCCACAAGTGAAAGAGGACGCGACGAAACTGGCTGCTAATTGGAAAGCCAAGATGACAGTGGCGAGTGACAATGAGTTGGAGGTCTTGGGATTTTTGTGGCTTGTTGCTGCTTATGACTTAACTTCTATCTACGATGCGAAGGAGCTTAAAAGGCTTCTTTCTAAAGTTTCGCGGGGTGAACAAGCAGCTCAAATAGGCCTGGCCCTTGGTATTCCGGCACCTGGAAGCCGCAACATTTGTTCCCCTGTCAAAATTGAGGAACCAGAATCTTCTACGGCCAACAATGCAGCAACTATTTCTTCTCCTAATCCTCAAACAAGTGCCACCACAGATGCAAGGGATTCACGGGGGTGTATAAATGAGACTTTGAATTGGAATGTAGATACAATACAGAATGAAATAGTGGCTTCTGTTCAATCGGCATCGGACCCAGAAGAACTTGTGTTGAAGATGATGCAAAATTCTCTTGGCAAATACTGGACAAGCACAGAAGATGGTCTGAAAAAGCATGTCATGTCGTGTAATATTTCTCTATTAAAGATGCTAATGAGAGTCTCACCACAAGTTGGATCTCACGTAAAAGAAGATGCCAAAAAGCTAGGACTCCAATGGAAAGCAAAAATTAGAGCTGATGCTGAACATTTGGAACATCTTTTGGAGATTGTGGGGTTTTTGCTATTTATTGTTGCATATGGATTGCTTCCTACACTAAATGGAGATGAGATTGTAAAGTTTCTTGAGAAGCTTTCTCAATATAAAGAAGCTGTAGAATCATGTCAGATGCATGGTTTTCTAGATAAGATCCTTGCTGTTTTTATTCAGACACTAGTGGAAAGGAAGCAACTCTTTCAGGCTCTTGGATTTGTCTTTAAGTTCAAGTTACGGGACAAGTTCACTCCAGTACGAGTCTTAAAAGACTATGTGAAGGATGCAATGAAGTGTTGGTCAGAAACTTTGAAGAGAAAGAAATCAGTTGATGAAAAGGTTGAGTTTTTAGACAGCAAAATAGCTGCTTTACGAACTGTGCTTCGATGCATCAAAGAATACAACCTCGAGTCTGAATACCCGTCCAGGGAAATTGCAGTACAAATAGGTGAGCTGGAAAAACTAAAGGAGATTTGGAGAAGTTCGGCAAAACATCTTGCCTCTGTCACTAGACAGCAAGAGAGTCAAGGGAAGAAACGTAGTAGCAGCACTTGTTCCCCTGCGGTTCAACAAGGACAACAGCAGAAAAGTAAATTCCATCGGACAGCTGAAGCAGCCCCTAGTCCCTATAAATCACCAACTTTCACCCCTGTGCTTCTGCAGTCAAGACCATCATCATCTTTGGCGTATGGAAATTATGGACAGCATGGGCAGGTTGGTGATATGGCTGCCAACTCTAGTGAAGTTGATAGATCTTCATCTCTGGCATATGGAAATTACGGACAGCTTGGGCAGTTTGGTGATATGGCTGCCAACTCTAGTGAAGTTGATAGATCTTCATCTTTGGTGAGTGAAATTCATGGACAGCATGGGCAGTTTGGTTATATGGCTGCCAACTTAGCTGAAGTTAATCCTCGTTTTGGTACCATAGATGAAGTTGATCCTCATTTTGGTGTACATAATCTTCCTAACCCTTATTCATCCATGGATTTCACCTTCTTCCCTGGCCGTTATGGCCCTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

950

Amino Acids

108.83

Weight (kDa)

5.62

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 332 - 464 6.7e-21 Frigida-like protein
Frigida PF07899 520 - 766 2.5e-47 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2530
Acc65I GGTACC 1 cut(s) 2750
AccB1I GGYRCC 2 cut(s) 1399, 2750
AccB7I CCANNNNNTGG 1 cut(s) 1748
AccBSI CCGCTC 1 cut(s) 292
AccI GTMKAC 1 cut(s) 146
AccII CGCG 4 cut(s) 62, 192, 1202, 1354
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 9 cut(s) 11, 14, 17, 62, 206, 292, 1354, 1411, 2390
AclWI GGATC 5 cut(s) 195, 942, 1759, 1978, 2762
AcoI YGGCCR 2 cut(s) 1458, 2833
AcsI RAATTY 3 cut(s) 1642, 2420, 2682
AcuI CTGAAG 6 cut(s) 122, 254, 746, 1068, 2457, 2751
AfaI GTAC 6 cut(s) 960, 1054, 2085, 2268, 2752, 2784
AfiI CCNNNNNNNGG 9 cut(s) 61, 175, 871, 955, 1403, 1748, 2254, 2747, 2777
AhlI ACTAGT 1 cut(s) 2008
AjnI CCWGG 4 cut(s) 1380, 1402, 2252, 2830
AjuI GAANNNNNNNTTGG 2 cut(s) 1088, 1120
AleI CACNNNNGTG 2 cut(s) 1531, 2477
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 2 cut(s) 1538, 1741
AlwI GGATC 5 cut(s) 195, 942, 1759, 1978, 2762
Ama87I CYCGRG 3 cut(s) 972, 1091, 2234
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 5 cut(s) 1378, 1383, 1458, 2833, 2842
ApoI RAATTY 3 cut(s) 1642, 2420, 2682
Asp700I GAANNNNTTC 2 cut(s) 713, 1042
Asp718I GGTACC 1 cut(s) 2750
AspA2I CCTAGG 1 cut(s) 55
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 5 cut(s) 133, 165, 1384, 1609, 2843
AsuC2I CCSGG 6 cut(s) 151, 169, 973, 974, 1092, 1093
AvaI CYCGRG 3 cut(s) 972, 1091, 2234
AvaII GGWCC 3 cut(s) 133, 165, 1609
AvrII CCTAGG 1 cut(s) 55
BanI GGYRCC 2 cut(s) 1399, 2750
BarI GAAGNNNNNNTAC 2 cut(s) 2775, 2807
Bbv12I GWGCWC 1 cut(s) 162
BccI CCATC 8 cut(s) 205, 232, 436, 780, 989, 1670, 2433, 2506
BceAI ACGGC 2 cut(s) 1473, 2820
BciT130I CCWGG 4 cut(s) 1382, 1404, 2254, 2832
BcnI CCSGG 6 cut(s) 151, 169, 973, 974, 1092, 1093
BcoDI GTCTC 2 cut(s) 1538, 1741
BcuI ACTAGT 1 cut(s) 2008
BfmI CTRYAG 3 cut(s) 1031, 1947, 2486
BfuAI ACCTGC 1 cut(s) 2530
BglI GCCNNNNNGGC 1 cut(s) 2841
BglII AGATCT 2 cut(s) 2579, 2660
BlnI CCTAGG 1 cut(s) 55
Bme18I GGWCC 3 cut(s) 133, 165, 1609
BmeT110I CYCGRG 3 cut(s) 972, 1091, 2234
BmgT120I GGNCC 5 cut(s) 133, 165, 1384, 1609, 2843
BmiI GGNNCC 6 cut(s) 53, 167, 1401, 1443, 1611, 2752
BplI GAGNNNNNCTC 2 cut(s) 1152, 1184
BpmI CTGGAG 3 cut(s) 149, 932, 2064
Bpu10I CCTNAGC 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 1940
BpuMI CCSGG 6 cut(s) 151, 169, 973, 974, 1092, 1093
BsaAI YACGTR 1 cut(s) 1759
BsaBI GATNNNNATC 2 cut(s) 2584, 2665
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 6 cut(s) 248, 278, 374, 404, 731, 761
Bsc4I CCNNNNNNNGG 9 cut(s) 61, 175, 871, 955, 1403, 1748, 2254, 2747, 2777
Bse1I ACTGG 3 cut(s) 1215, 1664, 2081
Bse3DI GCAATG 2 cut(s) 1119, 2121
Bse8I GATNNNNATC 2 cut(s) 2584, 2665
BseAI TCCGGA 1 cut(s) 162
BseBI CCWGG 4 cut(s) 1382, 1404, 2254, 2832
BseGI GGATG 5 cut(s) 791, 1000, 1114, 2116, 2807
BseJI GATNNNNATC 2 cut(s) 2584, 2665
BseLI CCNNNNNNNGG 9 cut(s) 61, 175, 871, 955, 1403, 1748, 2254, 2747, 2777
BseMI GCAATG 2 cut(s) 1119, 2121
BseMII CTCAG 2 cut(s) 530, 644
BseNI ACTGG 3 cut(s) 1215, 1664, 2081
Bsh1236I CGCG 4 cut(s) 62, 192, 1202, 1354
BshFI GGCC 5 cut(s) 1380, 1385, 1460, 2835, 2844
BshNI GGYRCC 2 cut(s) 1399, 2750
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 3 cut(s) 972, 1091, 2234
BsiSI CCGG 6 cut(s) 151, 163, 169, 973, 1092, 1397
BslFI GGGAC 2 cut(s) 2081, 2436
BslI CCNNNNNNNGG 9 cut(s) 61, 175, 871, 955, 1403, 1748, 2254, 2747, 2777
BsmAI GTCTC 2 cut(s) 1538, 1741
BsmFI GGGAC 2 cut(s) 2081, 2436
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 5 cut(s) 1380, 1385, 1460, 2835, 2844
BsoBI CYCGRG 3 cut(s) 972, 1091, 2234
Bsp1286I GDGCHC 1 cut(s) 162
Bsp13I TCCGGA 1 cut(s) 162
Bsp1407I TGTACA 1 cut(s) 2782
Bsp143I GATC 8 cut(s) 187, 934, 984, 1751, 1983, 2579, 2660, 2767
Bsp19I CCATGG 2 cut(s) 1047, 2811
BspACI CCGC 9 cut(s) 11, 14, 17, 62, 206, 292, 1354, 1411, 2390
BspANI GGCC 5 cut(s) 1380, 1385, 1460, 2835, 2844
BspCNI CTCAG 2 cut(s) 531, 645
BspEI TCCGGA 1 cut(s) 162
BspFNI CGCG 4 cut(s) 62, 192, 1202, 1354
BspLI GGNNCC 6 cut(s) 53, 167, 1401, 1443, 1611, 2752
BspMAI CTGCAG 2 cut(s) 1035, 2490
BspMI ACCTGC 1 cut(s) 2530
BspPI GGATC 5 cut(s) 195, 942, 1759, 1978, 2762
BspT107I GGYRCC 2 cut(s) 1399, 2750
BsrBI CCGCTC 1 cut(s) 292
BsrDI GCAATG 2 cut(s) 1119, 2121
BsrGI TGTACA 1 cut(s) 2782
BsrI ACTGG 3 cut(s) 1215, 1664, 2081
BssMI GATC 8 cut(s) 187, 934, 984, 1751, 1983, 2579, 2660, 2767
BssT1I CCWWGG 4 cut(s) 55, 1047, 1387, 2811
Bst2UI CCWGG 4 cut(s) 1382, 1404, 2254, 2832
Bst4CI ACNGT 4 cut(s) 112, 1062, 1240, 2206
Bst6I CTCTTC 4 cut(s) 596, 994, 1087, 2135
BstAUI TGTACA 1 cut(s) 2782
BstBAI YACGTR 1 cut(s) 1759
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 4 cut(s) 371, 539, 653, 2725
BstDSI CCRYGG 2 cut(s) 1047, 2811
BstENI CCTNNNNNAGG 1 cut(s) 869
BstF5I GGATG 5 cut(s) 791, 1000, 1114, 2116, 2807
BstFNI CGCG 4 cut(s) 62, 192, 1202, 1354
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 8 cut(s) 190, 937, 987, 1754, 1986, 2582, 2663, 2770
BstMAI GTCTC 2 cut(s) 1538, 1741
BstMBI GATC 8 cut(s) 187, 934, 984, 1751, 1983, 2579, 2660, 2767
BstMWI GCNNNNNNNGC 5 cut(s) 1333, 1466, 2441, 2726, 2841
BstNI CCWGG 4 cut(s) 1382, 1404, 2254, 2832
BstNSI RCATGY 2 cut(s) 455, 1694
BstSFI CTRYAG 3 cut(s) 1031, 1947, 2486
BstUI CGCG 4 cut(s) 62, 192, 1202, 1354
BstX2I RGATCY 5 cut(s) 934, 1751, 1983, 2579, 2660
BstYI RGATCY 5 cut(s) 934, 1751, 1983, 2579, 2660
BsuRI GGCC 5 cut(s) 1380, 1385, 1460, 2835, 2844
BtgI CCRYGG 2 cut(s) 1047, 2811
BtsCI GGATG 5 cut(s) 791, 1000, 1114, 2116, 2807
BtsIMutI CAGTG 1 cut(s) 1245
BveI ACCTGC 1 cut(s) 2530
Cac8I GCNNGC 1 cut(s) 87
CfoI GCGC 1 cut(s) 62
Cfr13I GGNCC 5 cut(s) 133, 165, 1384, 1609, 2843
Cfr9I CCCGGG 2 cut(s) 972, 1091
CseI GACGC 1 cut(s) 1208
Csp6I GTAC 6 cut(s) 959, 1053, 2084, 2267, 2751, 2783
CviQI GTAC 6 cut(s) 959, 1053, 2084, 2267, 2751, 2783
DdeI CTNAG 4 cut(s) 371, 539, 653, 2725
DpnI GATC 8 cut(s) 189, 936, 986, 1753, 1985, 2581, 2662, 2769
DpnII GATC 8 cut(s) 187, 934, 984, 1751, 1983, 2579, 2660, 2767
EaeI YGGCCR 2 cut(s) 1458, 2833
Eam1104I CTCTTC 4 cut(s) 596, 994, 1087, 2135
EarI CTCTTC 4 cut(s) 596, 994, 1087, 2135
EciI GGCGGA 2 cut(s) 32, 221
Eco130I CCWWGG 4 cut(s) 55, 1047, 1387, 2811
Eco147I AGGCCT 1 cut(s) 1380
Eco47I GGWCC 3 cut(s) 133, 165, 1609
Eco57I CTGAAG 6 cut(s) 122, 254, 746, 1068, 2457, 2751
Eco88I CYCGRG 3 cut(s) 972, 1091, 2234
EcoNI CCTNNNNNAGG 1 cut(s) 869
EcoRII CCWGG 4 cut(s) 1380, 1402, 2252, 2830
EcoT14I CCWWGG 4 cut(s) 55, 1047, 1387, 2811
EcoT22I ATGCAT 2 cut(s) 1968, 2219
ErhI CCWWGG 4 cut(s) 55, 1047, 1387, 2811
FalI AAGNNNNNCTT 2 cut(s) 2012, 2044
FaqI GGGAC 2 cut(s) 2081, 2436
FauI CCCGC 1 cut(s) 1347
FauNDI CATATG 2 cut(s) 1873, 2596
FblI GTMKAC 1 cut(s) 146
FokI GGATG 5 cut(s) 798, 1007, 1121, 2123, 2794
GlaI GCGC 1 cut(s) 61
GsuI CTGGAG 3 cut(s) 149, 932, 2064
HaeIII GGCC 5 cut(s) 1380, 1385, 1460, 2835, 2844
HapII CCGG 6 cut(s) 151, 163, 169, 973, 1092, 1397
HgaI GACGC 1 cut(s) 1208
HhaI GCGC 1 cut(s) 62
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HindIII AAGCTT 2 cut(s) 87, 1925
HpaII CCGG 6 cut(s) 151, 163, 169, 973, 1092, 1397
Hpy166II GTNNAC 5 cut(s) 108, 147, 1361, 2076, 2783
Hpy188III TCNNGA 5 cut(s) 163, 949, 1919, 1976, 2493
Hpy8I GTNNAC 5 cut(s) 108, 147, 1361, 2076, 2783
Hpy99I CGWCG 2 cut(s) 97, 1207
HpyCH4III ACNGT 4 cut(s) 112, 1062, 1240, 2206
HpyCH4IV ACGT 2 cut(s) 1758, 2365
HpyF10VI GCNNNNNNNGC 5 cut(s) 1333, 1466, 2441, 2726, 2841
HpyF3I CTNAG 4 cut(s) 371, 539, 653, 2725
HpySE526I ACGT 2 cut(s) 1758, 2365
HspAI GCGC 1 cut(s) 60
Kpn2I TCCGGA 1 cut(s) 162
KpnI GGTACC 1 cut(s) 2754
Kzo9I GATC 8 cut(s) 187, 934, 984, 1751, 1983, 2579, 2660, 2767
LmnI GCTCC 9 cut(s) 51, 124, 165, 289, 367, 535, 775, 838, 1324
MaeII ACGT 2 cut(s) 1758, 2365
MaeIII GTNAC 4 cut(s) 915, 1247, 2061, 2332
MalI GATC 8 cut(s) 189, 936, 986, 1753, 1985, 2581, 2662, 2769
MbiI CCGCTC 1 cut(s) 292
MboI GATC 8 cut(s) 187, 934, 984, 1751, 1983, 2579, 2660, 2767
MflI RGATCY 5 cut(s) 934, 1751, 1983, 2579, 2660
MhlI GDGCHC 1 cut(s) 162
MlyI GAGTC 9 cut(s) 80, 152, 341, 1073, 1743, 1779, 2097, 2246, 2359
MmeI TCCRAC 3 cut(s) 780, 1239, 1729
Mph1103I ATGCAT 2 cut(s) 1968, 2219
MroI TCCGGA 1 cut(s) 162
MroXI GAANNNNTTC 2 cut(s) 713, 1042
MseI TTAA 8 cut(s) 1074, 1301, 1329, 1718, 2052, 2093, 2736, 2851
MslI CAYNNNNRTG 3 cut(s) 1531, 2477, 2778
MspA1I CMGCKG 1 cut(s) 2435
MspI CCGG 6 cut(s) 151, 163, 169, 973, 1092, 1397
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 4 cut(s) 1382, 1404, 2254, 2832
MvnI CGCG 4 cut(s) 62, 192, 1202, 1354
MwoI GCNNNNNNNGC 5 cut(s) 1333, 1466, 2441, 2726, 2841
NciI CCSGG 6 cut(s) 151, 169, 973, 974, 1092, 1093
NcoI CCATGG 2 cut(s) 1047, 2811
NdeI CATATG 2 cut(s) 1873, 2596
NdeII GATC 8 cut(s) 187, 934, 984, 1751, 1983, 2579, 2660, 2767
NlaIV GGNNCC 6 cut(s) 53, 167, 1401, 1443, 1611, 2752
NmuCI GTSAC 2 cut(s) 1247, 2332
NsiI ATGCAT 2 cut(s) 1968, 2219
NspI RCATGY 2 cut(s) 455, 1694
OliI CACNNNNGTG 2 cut(s) 1531, 2477
PaeR7I CTCGAG 1 cut(s) 2234
PceI AGGCCT 1 cut(s) 1380
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PctI GAATGC 1 cut(s) 940
PdmI GAANNNNTTC 2 cut(s) 713, 1042
PflMI CCANNNNNTGG 1 cut(s) 1748
PleI GAGTC 9 cut(s) 79, 151, 340, 1073, 1742, 1779, 2096, 2245, 2358
PpsI GAGTC 9 cut(s) 79, 151, 340, 1073, 1742, 1779, 2096, 2245, 2358
Ppu21I YACGTR 1 cut(s) 1759
Psp6I CCWGG 4 cut(s) 1380, 1402, 2252, 2830
PspGI CCWGG 4 cut(s) 1380, 1402, 2252, 2830
PspN4I GGNNCC 6 cut(s) 53, 167, 1401, 1443, 1611, 2752
PspPI GGNCC 5 cut(s) 133, 165, 1384, 1609, 2843
PspXI VCTCGAGB 1 cut(s) 2234
PstI CTGCAG 2 cut(s) 1035, 2490
PsuI RGATCY 5 cut(s) 934, 1751, 1983, 2579, 2660
PvuII CAGCTG 1 cut(s) 2435
RsaI GTAC 6 cut(s) 960, 1054, 2085, 2268, 2752, 2784
RsaNI GTAC 6 cut(s) 959, 1053, 2084, 2267, 2751, 2783
RseI CAYNNNNRTG 3 cut(s) 1531, 2477, 2778
SalI GTCGAC 1 cut(s) 145
SaqAI TTAA 8 cut(s) 1074, 1301, 1329, 1718, 2052, 2093, 2736, 2851
Sau3AI GATC 8 cut(s) 187, 934, 984, 1751, 1983, 2579, 2660, 2767
Sau96I GGNCC 5 cut(s) 133, 165, 1384, 1609, 2843
SchI GAGTC 9 cut(s) 80, 152, 341, 1073, 1743, 1779, 2097, 2246, 2359
SduI GDGCHC 1 cut(s) 162
SfcI CTRYAG 3 cut(s) 1031, 1947, 2486
SfiI GGCCNNNNNGGCC 1 cut(s) 2841
Sfr274I CTCGAG 1 cut(s) 2234
SinI GGWCC 3 cut(s) 133, 165, 1609
SlaI CTCGAG 1 cut(s) 2234
SmaI CCCGGG 2 cut(s) 974, 1093
SmiMI CAYNNNNRTG 3 cut(s) 1531, 2477, 2778
SmlI CTYRAG 2 cut(s) 1919, 2234
SmoI CTYRAG 2 cut(s) 1919, 2234
SpeI ACTAGT 1 cut(s) 2008
SseBI AGGCCT 1 cut(s) 1380
SsiI CCGC 9 cut(s) 11, 14, 17, 62, 206, 292, 1354, 1411, 2390
SspI AATATT 1 cut(s) 1708
StuI AGGCCT 1 cut(s) 1380
StyI CCWWGG 4 cut(s) 55, 1047, 1387, 2811
TaaI ACNGT 4 cut(s) 112, 1062, 1240, 2206
TaiI ACGT 2 cut(s) 1761, 2368
TaqI TCGA 9 cut(s) 95, 141, 146, 174, 276, 671, 806, 2212, 2235
TatI WGTACW 2 cut(s) 2266, 2782
TauI GCSGC 4 cut(s) 14, 17, 65, 1413
Tru1I TTAA 8 cut(s) 1074, 1301, 1329, 1718, 2052, 2093, 2736, 2851
Tru9I TTAA 8 cut(s) 1074, 1301, 1329, 1718, 2052, 2093, 2736, 2851
TscAI CASTG 1 cut(s) 1245
TseFI GTSAC 2 cut(s) 1247, 2332
Tsp45I GTSAC 2 cut(s) 1247, 2332
TspGWI ACGGA 1 cut(s) 2622
TspMI CCCGGG 2 cut(s) 972, 1091
TspRI CASTG 1 cut(s) 1245
Van91I CCANNNNNTGG 1 cut(s) 1748
VpaK11BI GGWCC 3 cut(s) 133, 165, 1609
XagI CCTNNNNNAGG 1 cut(s) 869
XapI RAATTY 3 cut(s) 1642, 2420, 2682
XbaI TCTAGA 1 cut(s) 1975
XceI RCATGY 2 cut(s) 455, 1694
XcmI CCANNNNNNNNNTGG 1 cut(s) 1237
XhoI CTCGAG 1 cut(s) 2234
XmaI CCCGGG 2 cut(s) 972, 1091
XmaJI CCTAGG 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 2 cut(s) 713, 1042
Zsp2I ATGCAT 2 cut(s) 1968, 2219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.