MD14G1011100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000126
Physical Location & Seq
Reverse (-)
1070094 .. 1074495
4402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1011100.v1.1.491

Sequence Viewer

Length: 2700 bp
ATGGTGGTGAAAGGCAGGGAATTGCAGGACATTGTGCGGGAGGTTGAAGAAGGTAAGTCCCAGTTGCAGTCTATTAAGTTATCTTTCGAAAAGTATTCGGTTGAAGAAGGTAAGCTAGGAGAATTACAGAAATTGGTTAAGGGTAAGGAGATGGAGTATTATAATATGCAACAACGGTTGAAACAATTGTCGAAGGAGATTGAGTTAAAGGAGCGGGAGAAGGAGATCCGGTTCGCGGTCTTGGAAGCCAAGGACTCGGAATTGGACTCGGGTCTGGCGGCCAAAGGCAGGGAATTTGAGGACCTTGTGCAGGAGGTTGAAGAAGGTACGTCCCTATTGCAGTCTATTAAGTTATCTTTCGACAAGTATTCGGTTGAAGAAGGTAGATTAGGAGAATTACAGAAATTGGTTAAGGGTAAGGAGATGGAGTATTATAAGACACAACATCGGATGAAAGAACTGTCGAAAGAGATTGAGTCAAAGGAGCGGCAGTTAAATGCGGTTAGGGGTAAGGTTGCGGATCAATGGAAAGAGTTTGATTTGAGAGACGACGAAATTAGAGCTAAGCAAATATTGATTGAGGAATACGACAAAGAGGTGAAGTCAAGAGAGCATAAACTCGGTTTGATTGAGAAATTAATAGTAGAAAAAACGAATGTAGTTCAATCGAAAATGAAGGATTTGTGTGAGCTTCAGAAGTCAGTGGGGGTGTGGGACTGTAAACTTGAATTGGAACTTAAAAGACGTGTTGTGAAGGTCGAAAAACAATTTGAGTCAAAAGCCGACGAACTTAATTTGATTGACAGGAGGGTCAATGATTGCCTCAATGAGTCTCAGTTGAAAGAAAAGAATTTGGATTCCCACGAAAAGCATTTGGACTCAATCGACAAGTTGATACAGGAATCTGTGAGGCGGCTTGAACAAAAGACCAAAGAGCTTGTATTGAAGCAACAAGAATTTGAAAAGTCCAAAGAAGAACATATTCGCACAACTAAATCGAAGGAGAAGACTGATATACATCATACTCAAGTGAAGATTGAACAATCAGGAGATATCCCTGATAACATTGCCGCCCCTCCTTTTTGTGCAACCAATAATTCAAGAATCATTAGGGATGGTAGGAGCTTGCAGTTTTTCATGAATGATTATTTTAGGAGAACGGATTCAGTTTCTATCCAAGTTTCAGCCTGTCTTCATTTGTCATCAGACCCGGCAAAATTAGTTCTTGATGCTATGCAAGGGTTTTACCCTTCAAATTCAACTGTGGAAAACAGGGAATTTGATTTTGATTTGAGTGTAATTAGAAGGAGCTGTATTCATTTGTTAAAGGAGGTAAAGAGAGTCTCACCACAAATTAGTGAAAAGGTGAGAGCAGAAGCAAAGAAGTTGGCGGGTGAATGGAGAGCTAAGATGACTGTGGCGGCTGAGAACTGGTTGGAGGTTTTGGGTTTTTTGTGGCTTGTTATGGCATATGACTTGAACTCTATTTCTGGCAGGGAGGAGCTTCAGTCTCTTATCAATATAGCTGCTGATCACGAAAAACTGTCAGATGAATTGTACGGGATCCTTGACACCACAGATAACACACCTGCGAGCAGCAACCTTTGTTCCTTTATCAAAACTGATAAACCAGAATGTCTGCTGGGAAAGAATGCCGCAACTTGTTCTTCTCCAAATCTTGAACTAACTGCCACCACAGATGCTAGGAATTTGCAGGGGGGTCCAACTTGTTCTTCTTCAAATCTTCATCAAACTGCCACCACAGACGCTAGGAATTTCCAGGGGTTATCAAATGAGCATTCTAGTGGGAATCACTCAATACAGAATGAATTTTTACAGATTAACAAGTTGTTTTCTGTGTTAAATGGAGATGAGTTAGTAAATTATCTTGGGTTGATTTCGCAGAATAAACAGGCCCTAGAATATATTTGGAGTTCTGGTTGTGGAGATAAGATCCCTGATTGTATTCCGACTCTTATTGAATGGGGGCAACGGATTGAGGCTGTTAGAGTTATTTGCACCTTCAACTTAATTGACAAGTTTCCCCCGGGACCCCTCTTGAAAGAACATGTGGAGATTGCGAGGAAGTGTTCCCAGTCACTTATCTCGAAAACAAAACTACTTGGTGACGAGGTTGTAGACGAAGAAATAGCTGATCTAAGAGCTGTGATTGAATGCATTAAAGATTACGACCTAGAGTCGGAATACTCCCCCCGGGCGATTGAAACAAAAATAGTTAAGCTGCAGAAACAGAAGGACGAGTTGATACACAAGCGTCTTGCACCATGTCACAAGGTGAAACAACCGCGGAAAAAGAAGAATAAACGCACTGCCAAGTTTCAAGCACAACAGCTTAAATCTAAACGTCAGCGGGCATCAATATCAGCTTCCCCACCATATCCAATACCAAATCCCACCCCTGGATATGTATCGCCGTCATATTTGCCGTATGAAAACAGCGGACACCCCTGGAAACGTCAGCGGGCAGAAATATCATCTGCCGCACCACATCCAGTACCGCCGCTGTCATATTTGCCGCTGTCATATTTGCCGTATGAAAACATTCGACACCCCCGGCAATTCGGTATGGCTGCCAACGATGTAGGAATTTCGACAGGAACTCGTTACCATTGCGACGAGTTAGAGCATTTGGAACGAGTTGTCCCAAGTATTCACCCGAGAAGAAGAACGTGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida TEX13

Protein Analysis

900

Amino Acids

103.08

Weight (kDa)

7.08

Isoelectric Point (pI)

53.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 372 - 517 3.9e-25 Frigida-like protein
Frigida PF07899 616 - 754 4.4e-29 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 162, 435
AarI CACCTGC 1 cut(s) 1597
AasI GACNNNNNNGTC 1 cut(s) 809
Acc36I ACCTGC 1 cut(s) 1597
AccBSI CCGCTC 2 cut(s) 214, 487
AccI GTMKAC 1 cut(s) 2139
AccII CGCG 2 cut(s) 236, 2308
AclWI GGATC 5 cut(s) 220, 528, 1558, 1571, 1948
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 9 cut(s) 293, 850, 956, 1255, 1277, 1708, 1774, 1829, 2607
AcuI CTGAAG 2 cut(s) 677, 1490
AdeI CACNNNGTG 1 cut(s) 2296
AfaI GTAC 3 cut(s) 328, 1559, 2517
AfiI CCNNNNNNNGG 5 cut(s) 235, 288, 310, 2200, 2420
AflIII ACRYGT 2 cut(s) 745, 2068
AhdI GACNNNNNGTC 1 cut(s) 2197
AjiI CACGTC 1 cut(s) 746
AjnI CCWGG 3 cut(s) 1779, 2419, 2468
AjuI GAANNNNNNNTTGG 2 cut(s) 1717, 1749
AloI GAACNNNNNNTCC 4 cut(s) 215, 247, 2646, 2678
Alw26I GTCTC 4 cut(s) 540, 837, 1348, 1515
AlwI GGATC 5 cut(s) 220, 528, 1558, 1571, 1948
Ama87I CYCGRG 4 cut(s) 268, 2047, 2214, 2677
AoxI GGCC 2 cut(s) 279, 1914
ApeKI GCWGC 4 cut(s) 1526, 1596, 2242, 2591
ApoI RAATTY 9 cut(s) 293, 850, 956, 1255, 1277, 1708, 1774, 1829, 2607
AseI ATTAAT 1 cut(s) 638
Asp700I GAANNNNTTC 3 cut(s) 981, 1162, 2562
AspS9I GGNCC 4 cut(s) 301, 1721, 1915, 2051
AsuC2I CCSGG 6 cut(s) 1211, 2048, 2049, 2215, 2216, 2575
AsuHPI GGTGA 8 cut(s) 19, 610, 1338, 1378, 1406, 2138, 2308, 2666
AsuII TTCGAA 1 cut(s) 87
AvaI CYCGRG 4 cut(s) 268, 2047, 2214, 2677
AvaII GGWCC 3 cut(s) 301, 1721, 2051
BamHI GGATCC 1 cut(s) 1563
BbsI GAAGAC 2 cut(s) 1013, 1184
BbvI GCAGC 4 cut(s) 1513, 1608, 2229, 2578
BccI CCATC 3 cut(s) 145, 418, 1109
BceAI ACGGC 3 cut(s) 2419, 2431, 2536
BciT130I CCWGG 3 cut(s) 1781, 2421, 2470
BclI TGATCA 1 cut(s) 1531
BcnI CCSGG 6 cut(s) 1211, 2048, 2049, 2215, 2216, 2575
BcoDI GTCTC 4 cut(s) 540, 837, 1348, 1515
BfaI CTAG 6 cut(s) 116, 1704, 1770, 1803, 1919, 2195
BfmI CTRYAG 1 cut(s) 2243
BfuAI ACCTGC 1 cut(s) 1597
BlpI GCTNAGC 1 cut(s) 564
Bme1390I CCNGG 9 cut(s) 1211, 1781, 2048, 2049, 2215, 2216, 2421, 2470, 2575
Bme18I GGWCC 3 cut(s) 301, 1721, 2051
BmeRI GACNNNNNGTC 1 cut(s) 2197
BmeT110I CYCGRG 4 cut(s) 268, 2047, 2214, 2677
BmgBI CACGTC 1 cut(s) 746
BmgT120I GGNCC 4 cut(s) 301, 1721, 1915, 2051
BmiI GGNNCC 4 cut(s) 1565, 1722, 2052, 2053
BmrFI CCNGG 9 cut(s) 1211, 1781, 2048, 2049, 2215, 2216, 2421, 2470, 2575
BmrI ACTGGG 2 cut(s) 55, 2089
BmsI GCATC 3 cut(s) 1219, 1690, 2384
BmuI ACTGGG 2 cut(s) 55, 2089
BoxI GACNNNNGTC 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 1013, 1184
Bpu1102I GCTNAGC 1 cut(s) 564
Bpu14I TTCGAA 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 1011
BpuMI CCSGG 6 cut(s) 1211, 2048, 2049, 2215, 2216, 2575
BsaBI GATNNNNATC 2 cut(s) 1017, 2428
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 5 cut(s) 235, 288, 310, 2200, 2420
Bse1I ACTGG 4 cut(s) 61, 1436, 2095, 2513
Bse3DI GCAATG 2 cut(s) 1065, 2629
Bse8I GATNNNNATC 2 cut(s) 1017, 2428
BseBI CCWGG 3 cut(s) 1781, 2421, 2470
BseGI GGATG 4 cut(s) 456, 1120, 2509, 2700
BseJI GATNNNNATC 2 cut(s) 1017, 2428
BseLI CCNNNNNNNGG 5 cut(s) 235, 288, 310, 2200, 2420
BseMI GCAATG 2 cut(s) 1065, 2629
BseMII CTCAG 2 cut(s) 848, 1416
BseNI ACTGG 4 cut(s) 61, 1436, 2095, 2513
BseRI GAGGAG 1 cut(s) 1514
BseXI GCAGC 4 cut(s) 1513, 1608, 2229, 2578
BseYI CCCAGC 1 cut(s) 1642
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 2 cut(s) 236, 2308
BshFI GGCC 2 cut(s) 281, 1916
BsiHKCI CYCGRG 4 cut(s) 268, 2047, 2214, 2677
BsiSI CCGG 5 cut(s) 229, 1211, 2048, 2215, 2575
BslFI GGGAC 5 cut(s) 43, 316, 728, 2064, 2648
BslI CCNNNNNNNGG 5 cut(s) 235, 288, 310, 2200, 2420
BsmAI GTCTC 4 cut(s) 540, 837, 1348, 1515
BsmBI CGTCTC 1 cut(s) 540
BsmFI GGGAC 5 cut(s) 43, 316, 728, 2064, 2648
BsmI GAATGC 3 cut(s) 1657, 1798, 2180
BsnI GGCC 2 cut(s) 281, 1916
BsoBI CYCGRG 4 cut(s) 268, 2047, 2214, 2677
Bsp119I TTCGAA 1 cut(s) 87
Bsp143I GATC 6 cut(s) 225, 520, 1531, 1563, 1953, 2155
Bsp1720I GCTNAGC 1 cut(s) 564
BspANI GGCC 2 cut(s) 281, 1916
BspCNI CTCAG 2 cut(s) 847, 1417
BspFNI CGCG 2 cut(s) 236, 2308
BspHI TCATGA 1 cut(s) 1137
BspLI GGNNCC 4 cut(s) 1565, 1722, 2052, 2053
BspMAI CTGCAG 1 cut(s) 2247
BspMI ACCTGC 1 cut(s) 1597
BspPI GGATC 5 cut(s) 220, 528, 1558, 1571, 1948
BspT104I TTCGAA 1 cut(s) 87
BsrBI CCGCTC 2 cut(s) 214, 487
BsrDI GCAATG 2 cut(s) 1065, 2629
BsrI ACTGG 4 cut(s) 61, 1436, 2095, 2513
BssMI GATC 6 cut(s) 225, 520, 1531, 1563, 1953, 2155
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 3 cut(s) 1781, 2421, 2470
Bst4CI ACNGT 6 cut(s) 177, 462, 719, 1264, 1417, 1545
BstBI TTCGAA 1 cut(s) 87
BstC8I GCNNGC 4 cut(s) 1127, 1594, 2373, 2484
BstDEI CTNAG 5 cut(s) 564, 834, 1407, 1425, 2159
BstDSI CCRYGG 1 cut(s) 2306
BstENI CCTNNNNNAGG 1 cut(s) 308
BstF5I GGATG 4 cut(s) 456, 1120, 2509, 2700
BstFNI CGCG 2 cut(s) 236, 2308
BstKTI GATC 6 cut(s) 228, 523, 1534, 1566, 1956, 2158
BstMAI GTCTC 4 cut(s) 540, 837, 1348, 1515
BstMBI GATC 6 cut(s) 225, 520, 1531, 1563, 1953, 2155
BstNI CCWGG 3 cut(s) 1781, 2421, 2470
BstNSI RCATGY 1 cut(s) 2072
BstPAI GACNNNNGTC 1 cut(s) 270
BstSCI CCNGG 9 cut(s) 1209, 1779, 2046, 2047, 2213, 2214, 2419, 2468, 2573
BstSFI CTRYAG 1 cut(s) 2243
BstUI CGCG 2 cut(s) 236, 2308
BstV1I GCAGC 4 cut(s) 1513, 1608, 2229, 2578
BstV2I GAAGAC 2 cut(s) 1013, 1184
BstX2I RGATCY 3 cut(s) 225, 1563, 1953
BstYI RGATCY 3 cut(s) 225, 1563, 1953
BsuRI GGCC 2 cut(s) 281, 1916
BtgI CCRYGG 1 cut(s) 2306
BtrI CACGTC 1 cut(s) 746
BtsCI GGATG 4 cut(s) 456, 1120, 2509, 2700
BtsI GCAGTG 1 cut(s) 2328
BtsIMutI CAGTG 2 cut(s) 708, 2328
BveI ACCTGC 1 cut(s) 1597
Cac8I GCNNGC 4 cut(s) 1127, 1594, 2373, 2484
CciI TCATGA 1 cut(s) 1137
Cfr13I GGNCC 4 cut(s) 301, 1721, 1915, 2051
Cfr42I CCGCGG 1 cut(s) 2309
Cfr9I CCCGGG 2 cut(s) 2047, 2214
CseI GACGC 2 cut(s) 1775, 2264
Csp6I GTAC 3 cut(s) 327, 1558, 2516
CviAII CATG 3 cut(s) 1138, 2069, 2286
CviQI GTAC 3 cut(s) 327, 1558, 2516
DdeI CTNAG 5 cut(s) 564, 834, 1407, 1425, 2159
DpnI GATC 6 cut(s) 227, 522, 1533, 1565, 1955, 2157
DpnII GATC 6 cut(s) 225, 520, 1531, 1563, 1953, 2155
DraIII CACNNNGTG 1 cut(s) 2296
DrdI GACNNNNNNGTC 1 cut(s) 809
DriI GACNNNNNGTC 1 cut(s) 2197
DseDI GACNNNNNNGTC 1 cut(s) 809
EaeI YGGCCR 1 cut(s) 279
Eam1105I GACNNNNNGTC 1 cut(s) 2197
Eco130I CCWWGG 1 cut(s) 249
Eco32I GATATC 1 cut(s) 1054
Eco47I GGWCC 3 cut(s) 301, 1721, 2051
Eco57I CTGAAG 2 cut(s) 677, 1490
Eco88I CYCGRG 4 cut(s) 268, 2047, 2214, 2677
EcoNI CCTNNNNNAGG 1 cut(s) 308
EcoO109I RGGNCCY 3 cut(s) 301, 1915, 2051
EcoRII CCWGG 3 cut(s) 1779, 2419, 2468
EcoRV GATATC 1 cut(s) 1054
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 2180
ErhI CCWWGG 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 540
FaeI CATG 3 cut(s) 1141, 2072, 2289
FaqI GGGAC 5 cut(s) 43, 316, 728, 2064, 2648
FatI CATG 3 cut(s) 1137, 2068, 2285
FauI CCCGC 5 cut(s) 30, 207, 1384, 2364, 2475
FauNDI CATATG 1 cut(s) 1471
FbaI TGATCA 1 cut(s) 1531
FblI GTMKAC 1 cut(s) 2139
FokI GGATG 3 cut(s) 463, 1127, 2496
FspBI CTAG 6 cut(s) 116, 1704, 1770, 1803, 1919, 2195
GsaI CCCAGC 1 cut(s) 1646
HaeIII GGCC 2 cut(s) 281, 1916
HapII CCGG 5 cut(s) 229, 1211, 2048, 2215, 2575
HgaI GACGC 2 cut(s) 1775, 2264
Hin1II CATG 3 cut(s) 1141, 2072, 2289
HpaII CCGG 5 cut(s) 229, 1211, 2048, 2215, 2575
HphI GGTGA 8 cut(s) 19, 610, 1338, 1378, 1406, 2138, 2308, 2666
Hpy166II GTNNAC 2 cut(s) 722, 2140
Hpy188I TCNGA 7 cut(s) 259, 450, 696, 1207, 1549, 1971, 2203
Hpy188III TCNNGA 9 cut(s) 606, 1047, 1101, 1138, 1226, 1535, 1679, 2059, 2107
Hpy8I GTNNAC 2 cut(s) 722, 2140
Hpy99I CGWCG 3 cut(s) 554, 788, 2639
HpyCH4III ACNGT 6 cut(s) 177, 462, 719, 1264, 1417, 1545
HpyCH4IV ACGT 5 cut(s) 329, 745, 2365, 2476, 2690
HpyF3I CTNAG 5 cut(s) 564, 834, 1407, 1425, 2159
HpySE526I ACGT 5 cut(s) 329, 745, 2365, 2476, 2690
Hsp92II CATG 3 cut(s) 1141, 2072, 2289
KflI GGGWCCC 1 cut(s) 2051
Ksp22I TGATCA 1 cut(s) 1531
KspI CCGCGG 1 cut(s) 2309
Kzo9I GATC 6 cut(s) 225, 520, 1531, 1563, 1953, 2155
LmnI GCTCC 5 cut(s) 211, 484, 1122, 1308, 1501
Lsp1109I GCAGC 4 cut(s) 1513, 1608, 2229, 2578
LweI GCATC 3 cut(s) 1219, 1690, 2384
MaeI CTAG 6 cut(s) 116, 1704, 1770, 1803, 1919, 2195
MaeII ACGT 5 cut(s) 329, 745, 2365, 2476, 2690
MaeIII GTNAC 4 cut(s) 2097, 2126, 2288, 2624
MalI GATC 6 cut(s) 227, 522, 1533, 1565, 1955, 2157
MbiI CCGCTC 2 cut(s) 214, 487
MboI GATC 6 cut(s) 225, 520, 1531, 1563, 1953, 2155
MfeI CAATTG 1 cut(s) 185
MflI RGATCY 3 cut(s) 225, 1563, 1953
MlyI GAGTC 9 cut(s) 248, 260, 485, 782, 839, 872, 1350, 1966, 2207
MmeI TCCRAC 4 cut(s) 1416, 1748, 1994, 2181
Mph1103I ATGCAT 1 cut(s) 2180
MroXI GAANNNNTTC 3 cut(s) 981, 1162, 2562
MslI CAYNNNNRTG 1 cut(s) 1803
MspA1I CMGCKG 6 cut(s) 2308, 2371, 2460, 2482, 2524, 2539
MspI CCGG 5 cut(s) 229, 1211, 2048, 2215, 2575
MspR9I CCNGG 9 cut(s) 1211, 1781, 2048, 2049, 2215, 2216, 2421, 2470, 2575
MunI CAATTG 1 cut(s) 185
Mva1269I GAATGC 3 cut(s) 1657, 1798, 2180
MvaI CCWGG 3 cut(s) 1781, 2421, 2470
MvnI CGCG 2 cut(s) 236, 2308
NciI CCSGG 6 cut(s) 1211, 2048, 2049, 2215, 2216, 2575
NdeI CATATG 1 cut(s) 1471
NdeII GATC 6 cut(s) 225, 520, 1531, 1563, 1953, 2155
NlaIII CATG 3 cut(s) 1141, 2072, 2289
NlaIV GGNNCC 4 cut(s) 1565, 1722, 2052, 2053
NmuCI GTSAC 3 cut(s) 2097, 2126, 2288
NsiI ATGCAT 1 cut(s) 2180
NspI RCATGY 1 cut(s) 2072
NspV TTCGAA 1 cut(s) 87
PagI TCATGA 1 cut(s) 1137
PaqCI CACCTGC 1 cut(s) 1597
PciI ACATGT 1 cut(s) 2068
PctI GAATGC 3 cut(s) 1657, 1798, 2180
PdmI GAANNNNTTC 3 cut(s) 981, 1162, 2562
PfeI GAWTC 5 cut(s) 857, 902, 1104, 1163, 1810
PleI GAGTC 9 cut(s) 248, 260, 484, 781, 838, 872, 1349, 1966, 2206
PpsI GAGTC 9 cut(s) 248, 260, 484, 781, 838, 872, 1349, 1966, 2206
PpuMI RGGWCCY 2 cut(s) 301, 2051
PscI ACATGT 1 cut(s) 2068
PshAI GACNNNNGTC 1 cut(s) 270
PshBI ATTAAT 1 cut(s) 638
PsiI TTATAA 2 cut(s) 162, 435
Psp5II RGGWCCY 2 cut(s) 301, 2051
Psp6I CCWGG 3 cut(s) 1779, 2419, 2468
PspFI CCCAGC 1 cut(s) 1642
PspGI CCWGG 3 cut(s) 1779, 2419, 2468
PspN4I GGNNCC 4 cut(s) 1565, 1722, 2052, 2053
PspPI GGNCC 4 cut(s) 301, 1721, 1915, 2051
PspPPI RGGWCCY 2 cut(s) 301, 2051
PstI CTGCAG 1 cut(s) 2247
PsuI RGATCY 3 cut(s) 225, 1563, 1953
RsaI GTAC 3 cut(s) 328, 1559, 2517
RsaNI GTAC 3 cut(s) 327, 1558, 2516
RseI CAYNNNNRTG 1 cut(s) 1803
SacII CCGCGG 1 cut(s) 2309
Sau3AI GATC 6 cut(s) 225, 520, 1531, 1563, 1953, 2155
Sau96I GGNCC 4 cut(s) 301, 1721, 1915, 2051
SchI GAGTC 9 cut(s) 248, 260, 485, 782, 839, 872, 1350, 1966, 2207
ScrFI CCNGG 9 cut(s) 1211, 1781, 2048, 2049, 2215, 2216, 2421, 2470, 2575
SfaNI GCATC 3 cut(s) 1219, 1690, 2384
SfcI CTRYAG 1 cut(s) 2243
Sfr303I CCGCGG 1 cut(s) 2309
SfuI TTCGAA 1 cut(s) 87
SgrBI CCGCGG 1 cut(s) 2309
SinI GGWCC 3 cut(s) 301, 1721, 2051
SmaI CCCGGG 2 cut(s) 2049, 2216
SmiMI CAYNNNNRTG 1 cut(s) 1803
SmlI CTYRAG 1 cut(s) 1026
SmoI CTYRAG 1 cut(s) 1026
SspI AATATT 1 cut(s) 573
SspMI CTAG 6 cut(s) 116, 1704, 1770, 1803, 1919, 2195
StyD4I CCNGG 9 cut(s) 1209, 1779, 2046, 2047, 2213, 2214, 2419, 2468, 2573
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 6 cut(s) 177, 462, 719, 1264, 1417, 1545
TaiI ACGT 5 cut(s) 332, 748, 2368, 2479, 2693
TauI GCSGC 9 cut(s) 281, 490, 916, 1073, 1424, 1658, 2504, 2524, 2539
TfiI GAWTC 5 cut(s) 857, 902, 1104, 1163, 1810
TscAI CASTG 2 cut(s) 708, 2335
TseFI GTSAC 3 cut(s) 2097, 2126, 2288
TseI GCWGC 4 cut(s) 1526, 1596, 2242, 2591
Tsp45I GTSAC 3 cut(s) 2097, 2126, 2288
TspGWI ACGGA 2 cut(s) 1175, 2008
TspMI CCCGGG 2 cut(s) 2047, 2214
TspRI CASTG 2 cut(s) 708, 2335
VpaK11BI GGWCC 3 cut(s) 301, 1721, 2051
VspI ATTAAT 1 cut(s) 638
XagI CCTNNNNNAGG 1 cut(s) 308
XapI RAATTY 9 cut(s) 293, 850, 956, 1255, 1277, 1708, 1774, 1829, 2607
XceI RCATGY 1 cut(s) 2072
XmaI CCCGGG 2 cut(s) 2047, 2214
XmiI GTMKAC 1 cut(s) 2139
XmnI GAANNNNTTC 3 cut(s) 981, 1162, 2562
XspI CTAG 6 cut(s) 116, 1704, 1770, 1803, 1919, 2195
Zsp2I ATGCAT 1 cut(s) 2180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.