FvH4_6g04320

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000126
Physical Location & Seq
Forward (+)
2340197 .. 2346741
6545 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04320.t1

Sequence Viewer

Length: 3024 bp
ATGGAGCAAACGGCGGCGGAATTGGAGGAAATTGCGGAGGCGAAACAGAGGAGCCTCGGCGCGGCGTTCGAGGCCGCGAAATCCCAAGCTTCGTCGATTATTAAGTTCACCTTGGAGTGGAAGGAGCTCGAGGATCACTTCGAGTCGACCCGGACCGCGCTCAAAACCGGGTTCGAGGAGCTCGACGGACGCGTCAGGGAGGCGGCGGAGATGGAGGCCGAGGCGGAGAGGTTGATGGCGGTGGCGGAGCGGAGATGGGAGGAAGCGGAAGACGACCGCGTCAGGGAGGTGGCGGAGATGGAGGCCAAGGCGGAGAGGCTGACGGCGGAGATGGAGCGGAGGCGGAAGGAAGCGGAAGAAGGTGAGGGATATTTGGAGTCGGTGAGGGATTTGATTAGGGAGAATGATGAGGAGCTTAGGGTTAGGGAGGAGAGGTATGGGAGTTTGGAGAGGTTGATTAGGGAGAAGGAGAGGGAGGTGGAGGAGGTGGATAGGCATGTGAAGGAGAAGACTAGGAAGTTGAATTGGGTGGAGAAGAGGACAGAGGTGAAGGGGAAGGAGGTAGAGGATGAGGTGAAGAGGTTGAGTGGTGTGAAGAAGTTGGTTGAGGAGAGGAAGAAGGAGCTGATAATGGTGAATAAGGAATTGGAGGGGAAAGTGGAGATGCTGAGGTTTGTGGAGGACTCGATAGCGGAATGCTGTAGAGAGCTTGAGATTAAGGAGGGAGCGATTCGTGCGGCGGATGCGAAAGGGGAGGAGTTTTGTGTGTTGAAGAGAGCAATGGAGGAGTGGTGCTGTAATGTGGAAGTGAAGGAGAGGGAGATGGAAGGGTTGGTTGCGAGGATGGAGTCGAAGGAGAAGGAAGTGGAATCGAAGGTGGAGGAGCTTGGTTTGATTCGTAAGGAGTTTCTTGATGAGGTTCAGTTGAAGGAGAGGCATTTGGATTTGCTTGAGAAATCCTTACTAGAAAGGGAGGAGAAGTGGCATTTGGTGCAGAAGTCTGTAGAAGAATGCTCTCGGGGAAGTGTGATCACTAGGGATGGGATAGGATTGCAGCTGTTCATGGACGAGCATCTGAAGAGAATTGATTCCATGGGTACTGAACTCTCGGCTATTCTTAAAGAGTCTTCAGACCCGGTGAAATTGGTTTTGGATGCAATGCAGGGGTTTTACCCTGAGAATAGGGGGTCGGATTTTGAATTGAGAGTTAGAAGACGAAGTTGTTGCCTTTTGTTAGAGGAGTTGATGAGGATGTCCCCTCAAATGAATCCTCAAGTGAAAGAGGAGGCGACAAAAGTGGCTGCTGATTGGAAAGCCAAGATGACAGTGGCGAGTGACAATGACTATGAGGTTTTAGGTTTCTTGCGGCTTGTTGCTGCTTATGGCTTAACTTCTACCTATGATGCAAAGGAGCTTAAGAGGCTTCTTTCTAATGTTTCACATGGTCAACAAGCAGCTGAATTAGGCCTTGCGCTTGGTATTACGGCGCATGGGAGCCACATTTGTACCCCTGTCAAAATTGAGGAACCAGAATCTTCTGCAGCCAACAATGCAGCAACTGTTTCTTCTCCTAATCCTCAATCAAATGCCACCAGAGATGCAAGGAATTCACAGTTACCAGTATCTTCTGCAGCCAACAATACAGTACCTATTTCATCTCCTAATCCTCAGTCAAGTGCCACCACAGATGCAAGGAATTTTCAGGGATCTATAAATGAGCATTTGAGTTGGAATTTAGATGAAATACATAATGAATTAGTGGCTTCTGTTCGAAAGGCTCCAGACCCAGGAAAACTTGTGTTGATGCTGATGCAAAACTCTCTTGTCAAATACTGGACAAGCGCAGAAGCTAGTCTGAAAAAGGGTGCCATGTCATGTAATATTTCTCTATTAAAGATGCTGATGAGAGTCTCACCACAAGTAGGATCTCATGTAAAAGAAGATGCCAAGAAGCTAGGACTCCAATGGAAAGAAAATATTAGAGCTGCTGAACATCTGGAAAATCATTTGGAGATTGCGGGGTTTTTGCTGTTTGTTGCTACATATGGATTGATTTCTACACTGAATGGAGATGAGATTGTAAAGTTGCTCGAAAGGCTTTCTCAACATAAAGAGGCTTTTGAATCATTGCTGATGCATGGTTTTCTAGATAAGATCCTTGTGGTCTTTATTCAGACACTAGTGGAAGGGAAGCAACTCTTTCAGGCTCTTGGATTTGTCTTTAAGTTCAAGTTACTTGGCAACTTCACTCCAGTACGAGTCTTAAAAGACTATGTGAAGGATGTAAATTTGTCTGAAACTTTGAAGAGAAAGAACTCCGCTGATGAAAAGTTTGAGTTTATAGACAGCAGAATAGCTGATCTTAGAGCAGTGCTTCGATGCATCAAAGAATACAACCTCGAGTCCGAATACCCATCTAGGGAAATTGAAGTACAAATAGTTGAGTTGGAAAAACTAAAGGAGATTTGTAGAGGTCCGGAGAAATGTCTTGCCTCTGTCATTAGACAACAAGAGCAGAGTCAAGGGCAGAAACGTAGTAGCAGCAGTTCCCCCGCGGTTCAACAAGGACAACAGAAAAGTAAATTCCATCGGACAGCTGAAGCATCCCCAAGTCCCTATAAATCACCAAGTTTCACCCCTGTGCAACTGCAGTCAAGAGTCACAAGACCACCATCATCTTTGGCGTATGGAAATTATGGACAGCAAGGGCAGGTTGGTAATATGGCTGCAAACTCTAGTGAAGTTGATGGATCTTCATCTTTGGCATATGGAAATTATGGACCGCATGGGCGGGTCGGTGATATGGCCGCCAACTCCGTTCAAATTGATAGATTTTCATCTTTGGCGAGTGGAATTCATAGACAGCGTGGGCAGTTTGGTAATATGGCTGCCAACTTACCCGAAGTTAATCCCCATTTTGGTATCATAGATGAAGTTGATCCTCTTTTTGGGGTACCTAATCTTCCTAACCCTCATCCACTCATGGATTTCACTCCCTTTCCTCGCCGTTATGGCCCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

1008

Amino Acids

113.77

Weight (kDa)

5.53

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 347 - 495 1e-33 Frigida-like protein
Frigida PF07899 578 - 821 2e-44 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 191, 278
Acc36I ACCTGC 1 cut(s) 2701
Acc65I GGTACC 1 cut(s) 2953
AccB1I GGYRCC 2 cut(s) 1865, 2953
AccBSI CCGCTC 2 cut(s) 250, 337
AccI GTMKAC 1 cut(s) 146
AccII CGCG 6 cut(s) 62, 77, 158, 192, 279, 2555
AccIII TCCGGA 1 cut(s) 2476
AclWI GGATC 6 cut(s) 141, 1714, 1933, 2149, 2758, 2933
AcoI YGGCCR 1 cut(s) 2805
AcsI RAATTY 6 cut(s) 1606, 1696, 1732, 2287, 2582, 2853
AcuI CTGAAG 3 cut(s) 1097, 1113, 2619
AfaI GTAC 6 cut(s) 1099, 1507, 1647, 2256, 2433, 2955
AfiI CCNNNNNNNGG 8 cut(s) 61, 117, 283, 1787, 1922, 2419, 2918, 2948
AflII CTTAAG 1 cut(s) 1415
AflIII ACRYGT 1 cut(s) 190
AhlI ACTAGT 1 cut(s) 2179
AjnI CCWGG 1 cut(s) 1786
AjuI GAANNNNNNNTTGG 6 cut(s) 629, 661, 971, 1003, 1133, 1165
AleI CACNNNNGTG 1 cut(s) 2639
Alw21I GWGCWC 2 cut(s) 129, 183
Alw26I GTCTC 1 cut(s) 1915
AlwI GGATC 6 cut(s) 141, 1714, 1933, 2149, 2758, 2933
AlwNI CAGNNNCTG 1 cut(s) 1559
Ama87I CYCGRG 3 cut(s) 128, 1017, 2399
Aor13HI TCCGGA 1 cut(s) 2476
AoxI GGCC 6 cut(s) 72, 216, 303, 1465, 2805, 3013
ApoI RAATTY 6 cut(s) 1606, 1696, 1732, 2287, 2582, 2853
Asp700I GAANNNNTTC 1 cut(s) 1087
Asp718I GGTACC 1 cut(s) 2953
AspLEI GCGC 5 cut(s) 62, 160, 1474, 1489, 1844
AspS9I GGNCC 4 cut(s) 153, 2474, 2780, 3014
AsuC2I CCSGG 3 cut(s) 151, 169, 1136
AsuII TTCGAA 1 cut(s) 1771
AvaI CYCGRG 3 cut(s) 128, 1017, 2399
AvaII GGWCC 3 cut(s) 153, 2474, 2780
BaeI ACNNNNGTAYC 2 cut(s) 1489, 1522
BanI GGYRCC 2 cut(s) 1865, 2953
BanII GRGCYC 2 cut(s) 129, 183
BarI GAAGNNNNNNTAC 2 cut(s) 2946, 2978
BbsI GAAGAC 4 cut(s) 276, 515, 1119, 1219
Bbv12I GWGCWC 2 cut(s) 129, 183
BbvCI CCTCAGC 1 cut(s) 668
BceAI ACGGC 4 cut(s) 27, 339, 1500, 2991
BcgI CGANNNNNNTGC 2 cut(s) 1206, 1240
BciT130I CCWGG 1 cut(s) 1788
BclI TGATCA 1 cut(s) 1029
BcnI CCSGG 3 cut(s) 151, 169, 1136
BcoDI GTCTC 1 cut(s) 1915
BcuI ACTAGT 1 cut(s) 2179
BfaI CTAG 9 cut(s) 513, 965, 1035, 1851, 1955, 2147, 2180, 2418, 2736
BfmI CTRYAG 5 cut(s) 700, 1002, 1539, 1629, 2648
BfrI CTTAAG 1 cut(s) 1415
BfuAI ACCTGC 1 cut(s) 2701
BglI GCCNNNNNGGC 1 cut(s) 3012
Bme1390I CCNGG 4 cut(s) 151, 169, 1136, 1788
Bme18I GGWCC 3 cut(s) 153, 2474, 2780
BmeT110I CYCGRG 3 cut(s) 128, 1017, 2399
BmgT120I GGNCC 4 cut(s) 153, 2474, 2780, 3014
BmiI GGNNCC 6 cut(s) 53, 1496, 1527, 1779, 1867, 2955
BmrFI CCNGG 4 cut(s) 151, 169, 1136, 1788
BpiI GAAGAC 4 cut(s) 276, 515, 1119, 1219
BpmI CTGGAG 2 cut(s) 1764, 2235
Bpu10I CCTNAGC 2 cut(s) 416, 668
Bpu14I TTCGAA 1 cut(s) 1771
BpuEI CTTGAG 3 cut(s) 731, 971, 1257
BpuMI CCSGG 3 cut(s) 151, 169, 1136
BsaBI GATNNNNATC 2 cut(s) 2755, 2836
BsaJI CCNNGG 7 cut(s) 55, 111, 219, 306, 1092, 1786, 2553
BsaWI WCCGGW 1 cut(s) 2476
BsaXI ACNNNNNCTCC 6 cut(s) 419, 449, 776, 806, 965, 995
Bsc4I CCNNNNNNNGG 8 cut(s) 61, 117, 283, 1787, 1922, 2419, 2918, 2948
Bse1I ACTGG 3 cut(s) 1619, 1838, 2252
Bse3DI GCAATG 3 cut(s) 786, 1164, 2126
Bse8I GATNNNNATC 2 cut(s) 2755, 2836
BseAI TCCGGA 1 cut(s) 2476
BseBI CCWGG 1 cut(s) 1788
BseDI CCNNGG 7 cut(s) 55, 111, 219, 306, 1092, 1786, 2553
BseGI GGATG 9 cut(s) 574, 748, 849, 1045, 1159, 1257, 2287, 2603, 2974
BseJI GATNNNNATC 2 cut(s) 2755, 2836
BseLI CCNNNNNNNGG 8 cut(s) 61, 117, 283, 1787, 1922, 2419, 2918, 2948
BseMI GCAATG 3 cut(s) 786, 1164, 2126
BseMII CTCAG 3 cut(s) 659, 1167, 1682
BseNI ACTGG 3 cut(s) 1619, 1838, 2252
BsgI GTGCAG 1 cut(s) 1013
Bsh1236I CGCG 6 cut(s) 62, 77, 158, 192, 279, 2555
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 6 cut(s) 74, 218, 305, 1467, 2807, 3015
BshNI GGYRCC 2 cut(s) 1865, 2953
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 2 cut(s) 129, 183
BsiHKCI CYCGRG 3 cut(s) 128, 1017, 2399
BsiSI CCGG 4 cut(s) 151, 168, 1136, 2477
BslFI GGGAC 2 cut(s) 1240, 2598
BslI CCNNNNNNNGG 8 cut(s) 61, 117, 283, 1787, 1922, 2419, 2918, 2948
BsmAI GTCTC 1 cut(s) 1915
BsmFI GGGAC 2 cut(s) 1240, 2598
BsmI GAATGC 2 cut(s) 701, 1016
BsnI GGCC 6 cut(s) 74, 218, 305, 1467, 2807, 3015
BsoBI CYCGRG 3 cut(s) 128, 1017, 2399
Bsp119I TTCGAA 1 cut(s) 1771
Bsp1286I GDGCHC 2 cut(s) 129, 183
Bsp13I TCCGGA 1 cut(s) 2476
Bsp143I GATC 8 cut(s) 133, 1029, 1706, 1925, 2154, 2359, 2750, 2938
Bsp19I CCATGG 1 cut(s) 1092
BspANI GGCC 6 cut(s) 74, 218, 305, 1467, 2807, 3015
BspCNI CTCAG 3 cut(s) 660, 1168, 1681
BspEI TCCGGA 1 cut(s) 2476
BspFNI CGCG 6 cut(s) 62, 77, 158, 192, 279, 2555
BspLI GGNNCC 6 cut(s) 53, 1496, 1527, 1779, 1867, 2955
BspMAI CTGCAG 3 cut(s) 1543, 1633, 2652
BspMI ACCTGC 1 cut(s) 2701
BspPI GGATC 6 cut(s) 141, 1714, 1933, 2149, 2758, 2933
BspT104I TTCGAA 1 cut(s) 1771
BspT107I GGYRCC 2 cut(s) 1865, 2953
BspTI CTTAAG 1 cut(s) 1415
BsrBI CCGCTC 2 cut(s) 250, 337
BsrDI GCAATG 3 cut(s) 786, 1164, 2126
BsrI ACTGG 3 cut(s) 1619, 1838, 2252
BssECI CCNNGG 7 cut(s) 55, 111, 219, 306, 1092, 1786, 2553
BssMI GATC 8 cut(s) 133, 1029, 1706, 1925, 2154, 2359, 2750, 2938
BssT1I CCWWGG 3 cut(s) 111, 306, 1092
Bst2UI CCWGG 1 cut(s) 1788
Bst4CI ACNGT 4 cut(s) 1327, 1561, 1614, 1645
Bst6I CTCTTC 5 cut(s) 530, 572, 767, 1073, 2300
BstAFI CTTAAG 1 cut(s) 1415
BstAPI GCANNNNNTGC 1 cut(s) 991
BstBI TTCGAA 1 cut(s) 1771
BstDEI CTNAG 5 cut(s) 416, 668, 1176, 1668, 2363
BstDSI CCRYGG 2 cut(s) 1092, 2553
BstF5I GGATG 9 cut(s) 574, 748, 849, 1045, 1159, 1257, 2287, 2603, 2974
BstFNI CGCG 6 cut(s) 62, 77, 158, 192, 279, 2555
BstHHI GCGC 5 cut(s) 62, 160, 1474, 1489, 1844
BstKTI GATC 8 cut(s) 136, 1032, 1709, 1928, 2157, 2362, 2753, 2941
BstMAI GTCTC 1 cut(s) 1915
BstMBI GATC 8 cut(s) 133, 1029, 1706, 1925, 2154, 2359, 2750, 2938
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 8 cut(s) 71, 734, 743, 991, 1420, 1550, 2095, 3012
BstNI CCWGG 1 cut(s) 1788
BstNSI RCATGY 1 cut(s) 500
BstSCI CCNGG 4 cut(s) 149, 167, 1134, 1786
BstSFI CTRYAG 5 cut(s) 700, 1002, 1539, 1629, 2648
BstUI CGCG 6 cut(s) 62, 77, 158, 192, 279, 2555
BstV2I GAAGAC 4 cut(s) 276, 515, 1119, 1219
BstX2I RGATCY 4 cut(s) 1706, 1925, 2154, 2750
BstYI RGATCY 4 cut(s) 1706, 1925, 2154, 2750
BsuRI GGCC 6 cut(s) 74, 218, 305, 1467, 2807, 3015
BtgI CCRYGG 2 cut(s) 1092, 2553
BtsCI GGATG 9 cut(s) 574, 748, 849, 1045, 1159, 1257, 2287, 2603, 2974
BtsI GCAGTG 1 cut(s) 2376
BtsIMutI CAGTG 3 cut(s) 1332, 2060, 2376
BveI ACCTGC 1 cut(s) 2701
CaiI CAGNNNCTG 1 cut(s) 1559
CfoI GCGC 5 cut(s) 62, 160, 1474, 1489, 1844
Cfr13I GGNCC 4 cut(s) 153, 2474, 2780, 3014
Cfr42I CCGCGG 1 cut(s) 2556
CpoI CGGWCCG 1 cut(s) 153
CseI GACGC 3 cut(s) 181, 198, 268
Csp6I GTAC 6 cut(s) 1098, 1506, 1646, 2255, 2432, 2954
CspI CGGWCCG 1 cut(s) 153
CviQI GTAC 6 cut(s) 1098, 1506, 1646, 2255, 2432, 2954
DdeI CTNAG 5 cut(s) 416, 668, 1176, 1668, 2363
DpnI GATC 8 cut(s) 135, 1031, 1708, 1927, 2156, 2361, 2752, 2940
DpnII GATC 8 cut(s) 133, 1029, 1706, 1925, 2154, 2359, 2750, 2938
DrdI GACNNNNNNGTC 2 cut(s) 191, 278
DseDI GACNNNNNNGTC 2 cut(s) 191, 278
EaeI YGGCCR 1 cut(s) 2805
Eam1104I CTCTTC 5 cut(s) 530, 572, 767, 1073, 2300
EarI CTCTTC 5 cut(s) 530, 572, 767, 1073, 2300
EciI GGCGGA 9 cut(s) 32, 221, 239, 260, 308, 326, 341, 358, 755
Ecl136II GAGCTC 2 cut(s) 127, 181
Eco130I CCWWGG 3 cut(s) 111, 306, 1092
Eco147I AGGCCT 1 cut(s) 1467
Eco24I GRGCYC 2 cut(s) 129, 183
Eco47I GGWCC 3 cut(s) 153, 2474, 2780
Eco53kI GAGCTC 2 cut(s) 127, 181
Eco57I CTGAAG 3 cut(s) 1097, 1113, 2619
Eco88I CYCGRG 3 cut(s) 128, 1017, 2399
EcoICRI GAGCTC 2 cut(s) 127, 181
EcoRI GAATTC 2 cut(s) 1606, 2853
EcoRII CCWGG 1 cut(s) 1786
EcoT14I CCWWGG 3 cut(s) 111, 306, 1092
EcoT22I ATGCAT 2 cut(s) 2139, 2384
EcoT38I GRGCYC 2 cut(s) 129, 183
ErhI CCWWGG 3 cut(s) 111, 306, 1092
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 2183, 2215
FaqI GGGAC 2 cut(s) 1240, 2598
FauI CCCGC 3 cut(s) 2011, 2560, 2784
FauNDI CATATG 2 cut(s) 2044, 2767
FbaI TGATCA 1 cut(s) 1029
FblI GTMKAC 1 cut(s) 146
FokI GGATG 9 cut(s) 581, 755, 856, 1052, 1166, 1264, 2294, 2590, 2961
FriOI GRGCYC 2 cut(s) 129, 183
FspBI CTAG 9 cut(s) 513, 965, 1035, 1851, 1955, 2147, 2180, 2418, 2736
GlaI GCGC 5 cut(s) 61, 159, 1473, 1488, 1843
GsuI CTGGAG 2 cut(s) 1764, 2235
HaeIII GGCC 6 cut(s) 74, 218, 305, 1467, 2807, 3015
HapII CCGG 4 cut(s) 151, 168, 1136, 2477
HgaI GACGC 3 cut(s) 181, 198, 268
HhaI GCGC 5 cut(s) 62, 160, 1474, 1489, 1844
Hin6I GCGC 5 cut(s) 60, 158, 1472, 1487, 1842
HinP1I GCGC 5 cut(s) 60, 158, 1472, 1487, 1842
HincII GTYRAC 2 cut(s) 147, 1448
HindII GTYRAC 2 cut(s) 147, 1448
HindIII AAGCTT 1 cut(s) 87
HpaII CCGG 4 cut(s) 151, 168, 1136, 2477
Hpy166II GTNNAC 3 cut(s) 108, 147, 1448
Hpy188I TCNGA 8 cut(s) 1077, 1132, 1192, 1857, 2175, 2296, 2407, 2592
Hpy188III TCNNGA 6 cut(s) 911, 1781, 1997, 2147, 2477, 2655
Hpy8I GTNNAC 3 cut(s) 108, 147, 1448
Hpy99I CGWCG 2 cut(s) 97, 188
HpyCH4III ACNGT 4 cut(s) 1327, 1561, 1614, 1645
HpyCH4IV ACGT 1 cut(s) 2533
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 734, 743, 991, 1420, 1550, 2095, 3012
HpyF3I CTNAG 5 cut(s) 416, 668, 1176, 1668, 2363
HpySE526I ACGT 1 cut(s) 2533
HspAI GCGC 5 cut(s) 60, 158, 1472, 1487, 1842
Kpn2I TCCGGA 1 cut(s) 2476
KpnI GGTACC 1 cut(s) 2957
Ksp22I TGATCA 1 cut(s) 1029
KspI CCGCGG 1 cut(s) 2556
Kzo9I GATC 8 cut(s) 133, 1029, 1706, 1925, 2154, 2359, 2750, 2938
MaeI CTAG 9 cut(s) 513, 965, 1035, 1851, 1955, 2147, 2180, 2418, 2736
MaeII ACGT 1 cut(s) 2533
MaeIII GTNAC 4 cut(s) 1334, 1614, 2232, 2659
MalI GATC 8 cut(s) 135, 1031, 1708, 1927, 2156, 2361, 2752, 2940
MbiI CCGCTC 2 cut(s) 250, 337
MboI GATC 8 cut(s) 133, 1029, 1706, 1925, 2154, 2359, 2750, 2938
MflI RGATCY 4 cut(s) 1706, 1925, 2154, 2750
MhlI GDGCHC 2 cut(s) 129, 183
MluI ACGCGT 1 cut(s) 190
MmeI TCCRAC 3 cut(s) 1170, 1709, 2427
Mph1103I ATGCAT 2 cut(s) 2139, 2384
MroI TCCGGA 1 cut(s) 2476
MroXI GAANNNNTTC 1 cut(s) 1087
MslI CAYNNNNRTG 1 cut(s) 2639
MspA1I CMGCKG 5 cut(s) 1057, 1457, 2321, 2555, 2597
MspCI CTTAAG 1 cut(s) 1415
MspI CCGG 4 cut(s) 151, 168, 1136, 2477
MspR9I CCNGG 4 cut(s) 151, 169, 1136, 1788
Mva1269I GAATGC 2 cut(s) 701, 1016
MvaI CCWGG 1 cut(s) 1788
MvnI CGCG 6 cut(s) 62, 77, 158, 192, 279, 2555
MwoI GCNNNNNNNGC 8 cut(s) 71, 734, 743, 991, 1420, 1550, 2095, 3012
NciI CCSGG 3 cut(s) 151, 169, 1136
NcoI CCATGG 1 cut(s) 1092
NdeI CATATG 2 cut(s) 2044, 2767
NdeII GATC 8 cut(s) 133, 1029, 1706, 1925, 2154, 2359, 2750, 2938
NlaIV GGNNCC 6 cut(s) 53, 1496, 1527, 1779, 1867, 2955
NmeAIII GCCGAG 3 cut(s) 36, 244, 1088
NmuCI GTSAC 2 cut(s) 1334, 2659
NsiI ATGCAT 2 cut(s) 2139, 2384
NspI RCATGY 1 cut(s) 500
NspV TTCGAA 1 cut(s) 1771
OliI CACNNNNGTG 1 cut(s) 2639
PaeR7I CTCGAG 2 cut(s) 128, 2399
PceI AGGCCT 1 cut(s) 1467
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 189
PctI GAATGC 2 cut(s) 701, 1016
PdmI GAANNNNTTC 1 cut(s) 1087
PfeI GAWTC 7 cut(s) 730, 869, 895, 1088, 1267, 1532, 2123
PflFI GACNNNGTC 1 cut(s) 278
Psp124BI GAGCTC 2 cut(s) 129, 183
Psp6I CCWGG 1 cut(s) 1786
PspGI CCWGG 1 cut(s) 1786
PspN4I GGNNCC 6 cut(s) 53, 1496, 1527, 1779, 1867, 2955
PspPI GGNCC 4 cut(s) 153, 2474, 2780, 3014
PspXI VCTCGAGB 2 cut(s) 128, 2399
PsrI GAACNNNNNNTAC 2 cut(s) 2530, 2562
PstI CTGCAG 3 cut(s) 1543, 1633, 2652
PstNI CAGNNNCTG 1 cut(s) 1559
PsuI RGATCY 4 cut(s) 1706, 1925, 2154, 2750
PsyI GACNNNGTC 1 cut(s) 278
PvuII CAGCTG 3 cut(s) 1057, 1457, 2597
RsaI GTAC 6 cut(s) 1099, 1507, 1647, 2256, 2433, 2955
RsaNI GTAC 6 cut(s) 1098, 1506, 1646, 2255, 2432, 2954
RseI CAYNNNNRTG 1 cut(s) 2639
Rsr2I CGGWCCG 1 cut(s) 153
RsrII CGGWCCG 1 cut(s) 153
SacI GAGCTC 2 cut(s) 129, 183
SacII CCGCGG 1 cut(s) 2556
SalI GTCGAC 1 cut(s) 145
Sau3AI GATC 8 cut(s) 133, 1029, 1706, 1925, 2154, 2359, 2750, 2938
Sau96I GGNCC 4 cut(s) 153, 2474, 2780, 3014
ScrFI CCNGG 4 cut(s) 151, 169, 1136, 1788
SduI GDGCHC 2 cut(s) 129, 183
SfcI CTRYAG 5 cut(s) 700, 1002, 1539, 1629, 2648
Sfr274I CTCGAG 2 cut(s) 128, 2399
Sfr303I CCGCGG 1 cut(s) 2556
SfuI TTCGAA 1 cut(s) 1771
SgrBI CCGCGG 1 cut(s) 2556
SinI GGWCC 3 cut(s) 153, 2474, 2780
SlaI CTCGAG 2 cut(s) 128, 2399
SmiMI CAYNNNNRTG 1 cut(s) 2639
SmlI CTYRAG 6 cut(s) 128, 710, 950, 1272, 1415, 2399
SmoI CTYRAG 6 cut(s) 128, 710, 950, 1272, 1415, 2399
SpeI ACTAGT 1 cut(s) 2179
SseBI AGGCCT 1 cut(s) 1467
SspI AATATT 2 cut(s) 1882, 1978
SspMI CTAG 9 cut(s) 513, 965, 1035, 1851, 1955, 2147, 2180, 2418, 2736
SstI GAGCTC 2 cut(s) 129, 183
StuI AGGCCT 1 cut(s) 1467
StyD4I CCNGG 4 cut(s) 149, 167, 1134, 1786
StyI CCWWGG 3 cut(s) 111, 306, 1092
TaaI ACNGT 4 cut(s) 1327, 1561, 1614, 1645
TaiI ACGT 1 cut(s) 2536
TatI WGTACW 1 cut(s) 2431
TauI GCSGC 7 cut(s) 17, 65, 77, 206, 740, 1369, 2810
TfiI GAWTC 7 cut(s) 730, 869, 895, 1088, 1267, 1532, 2123
TscAI CASTG 3 cut(s) 1332, 2067, 2376
TseFI GTSAC 2 cut(s) 1334, 2659
Tsp45I GTSAC 2 cut(s) 1334, 2659
TspGWI ACGGA 2 cut(s) 201, 2806
TspRI CASTG 3 cut(s) 1332, 2067, 2376
Tth111I GACNNNGTC 1 cut(s) 278
Vha464I CTTAAG 1 cut(s) 1415
VpaK11BI GGWCC 3 cut(s) 153, 2474, 2780
XapI RAATTY 6 cut(s) 1606, 1696, 1732, 2287, 2582, 2853
XbaI TCTAGA 1 cut(s) 2146
XceI RCATGY 1 cut(s) 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 1324
XhoI CTCGAG 2 cut(s) 128, 2399
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 1087
XspI CTAG 9 cut(s) 513, 965, 1035, 1851, 1955, 2147, 2180, 2418, 2736
Zsp2I ATGCAT 2 cut(s) 2139, 2384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.