FvH4_3g28254

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000172
Physical Location & Seq
Reverse (-)
21247920 .. 21249621
1702 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28254.t1

Sequence Viewer

Length: 963 bp
ATGGGAACATGGGTCAATACAAAGGAAGAAATTTCTGATATTTTAGTTAAAGATTTTAAGAAAAGATTTCAGAAGGATACCACACTCAGTGACATTGCTATTACAAATGTCATTAATGTCATTGATCCTTGTATTGAAAATGCTGATAATAATCTTCTTTTAAAGCAGGTTACAGATGAGGAGATCTGGGATGCAGTTAAGTCTATTGGAGCCCTAAAAGCTCTAAGACCAGATGGAATTTTGTTGTGCGTTGCCTCCTCCATGGCAGTAGCTCTTGCCAAGATGACAGCCAGTCTCACTTCCAAACTGTCGCTGAAGGATGCTAAAGAGTCGATGGACTTGGGAAATCTTCGTCTACCACCCGGTTTGATGACGGAGGCGTCTGTGTGCCTCATTGGAGAAACAATCGGCCATGTTCTAGGCGTAGATGGACATGGAGTTCGCTTAGGAAACCCTAGGGTTAGGGTTTTGCTCCCTCTGAACGAACCAGTTAGGTTGTCTTGCCAGTCTCCGGTTAATGTGCTCCAACTCTTCTTCAGGTATGAGAGACTCCTTGGGCGGTGTCGTGATTGTGTTATGATTAATCACGGCGGTATTATGTACCCTAGTTTGGTGACTGCGGAGGCGGTTCTTGTGCCTCCATCACAGTCTGGTCCTAAGATGGTCTTTTGGGCGGCGTCTTCTAGCTTCTGGTTGTTGGATGAGGATGATGAAGCTGATACGGGAAAACATACCAAGCACTCTTTGGCCCTTGTTCCGTTTGTGCTTCAACCGGAGCAACTTGGCTTCAAGGAGAATATAAATGGTCTCGAGTTTACTAGATCTAGTGGTGCCAAGAAACAGGGATGTCCTCGGGGTAGTAAGAGCAAATCGAGAGTCGCTGCTGTTGTTCCTCTACCTTCGGAAGAGGGTGTGAACTTGGAGATTGAGGAGGGTGAGATCATTCCGGTTACTGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

321

Amino Acids

34.87

Weight (kDa)

5.43

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59650 AT3G59650
fragaria_vesca FvH4_1g19981 FvH4_1g22221 FvH4_2g13530 FvH4_2g13791 FvH4_2g14332 FvH4_2g14911 FvH4_2g16332 FvH4_3g10980 FvH4_3g11141 FvH4_3g14171 FvH4_3g26311 FvH4_3g28254 FvH4_3g29741 FvH4_4g05261 FvH4_4g12002 FvH4_4g13371 FvH4_4g15541 FvH4_4g18531 FvH4_5g02661 FvH4_5g02662 FvH4_5g16791 FvH4_5g29700 FvH4_5g35881 FvH4_5g37061 FvH4_6g01571 FvH4_6g12761 FvH4_6g14961 FvH4_6g20381 FvH4_6g22001 FvH4_6g23673 FvH4_6g23960 FvH4_6g28051 FvH4_6g31094 FvH4_6g36541 FvH4_7g00911 FvH4_7g06200 FvH4_7g06200 FvH4_7g09551
malus_domestica MD08G1230600.v1.1
prunus_persica Prupe.1G206900_v2.0.a1 Prupe.1G206900_v2.0.a1 Prupe.1G206900_v2.0.a1
pyrus_communis pycom08g20050 pycom15g37590
rosa_chinensis RchiOBHm_Chr1g0333831 RchiOBHm_Chr1g0344871 RchiOBHm_Chr3g0473241 RchiOBHm_Chr5g0018871 RchiOBHm_Chr6g0265791
rosa_laevigata RLG00000005720 RLG00000012718 RLG00000027174 RLG00000029225 RLG00000029527
rosa_multiflora Rmu_co8002714.1_g000001 Rmu_co8072270.1_g000001 Rmu_co8095594.1_g000001 Rmu_co8308871.1_g000001 Rmu_co8398699.1_g000001 Rmu_co8404287.1_g000001 Rmu_sc0000035.1_g000010 Rmu_sc0000133.1_g000007 Rmu_sc0000253.1_g000023 Rmu_sc0000308.1_g000010 Rmu_sc0000309.1_g000030 Rmu_sc0000515.1_g000015 Rmu_sc0000865.1_g000001 Rmu_sc0000889.1_g000005 Rmu_sc0001262.1_g000011 Rmu_sc0001518.1_g000013 Rmu_sc0001533.1_g000006 Rmu_sc0001547.1_g000007 Rmu_sc0001826.1_g000013 Rmu_sc0002060.1_g000017 Rmu_sc0002129.1_g000005 Rmu_sc0002449.1_g000016 Rmu_sc0002460.1_g000036 Rmu_sc0002475.1_g000014 Rmu_sc0002553.1_g000017 Rmu_sc0002759.1_g000033 Rmu_sc0002918.1_g000009 Rmu_sc0002955.1_g000020 Rmu_sc0003094.1_g000010 Rmu_sc0003669.1_g000009 Rmu_sc0004109.1_g000008 Rmu_sc0004350.1_g000007 Rmu_sc0004542.1_g000018 Rmu_sc0004967.1_g000005 Rmu_sc0005026.1_g000005 Rmu_sc0005369.1_g000013 Rmu_sc0005400.1_g000025 Rmu_sc0005420.1_g000006 Rmu_sc0005467.1_g000013 Rmu_sc0005497.1_g000003 Rmu_sc0005607.1_g000023 Rmu_sc0005949.1_g000008 Rmu_sc0005970.1_g000002 Rmu_sc0006452.1_g000001 Rmu_sc0006720.1_g000008 Rmu_sc0006867.1_g000004 Rmu_sc0007177.1_g000006 Rmu_sc0007199.1_g000011 Rmu_sc0007783.1_g000004 Rmu_sc0007885.1_g000006 Rmu_sc0008619.1_g000001 Rmu_sc0008709.1_g000004 Rmu_sc0008709.1_g000005 Rmu_sc0009185.1_g000029 Rmu_sc0010244.1_g000010 Rmu_sc0011610.1_g000005 Rmu_sc0011680.1_g000013 Rmu_sc0012075.1_g000003 Rmu_sc0012278.1_g000001 Rmu_sc0012628.1_g000001 Rmu_sc0018485.1_g000006 Rmu_sc0019960.1_g000001 Rmu_sc0020177.1_g000002 Rmu_sc0020656.1_g000001 Rmu_sc0021333.1_g000003 Rmu_sc0023170.1_g000002 Rmu_sc0023989.1_g000005 Rmu_sc0025259.1_g000001 Rmu_sc0025350.1_g000013 Rmu_sc0029678.1_g000002 Rmu_sc0035939.1_g000001 Rmu_sc0039260.1_g000001 Rmu_sc0039886.1_g000001 Rmu_ssc0000323.1_g000030 Rmu_ssc0000333.1_g000005 Rmu_ssc0000471.1_g000017 Rmu_ssc0000480.1_g000025
rosa_roxburghii Rroxscaffold_1G00006060 Rroxscaffold_1G00048570 Rroxscaffold_1G00057650 Rroxscaffold_1G00072720 Rroxscaffold_4G00281190 Rroxscaffold_4G00317160 Rroxscaffold_6G00411570
rosa_rugosa Rorug01G0004800 Rorug01G0112100 Rorug01G0206100 Rorug02G0324000 Rorug05G0305600 Rorug05G0326800 Rorug06G0018900 Rorug06G0167000 Rorug07G0031200 Rorug07G0071900
rosa_samantha Rh1AG134700 Rh1BG103000 Rh1CG007300 Rh1CG128100 Rh1DG140100 Rh2DG579500 Rh3DG199000 Rh4BG252500 Rh5AG374900 Rh5BG146800 Rh5BG151500 Rh5DG399700
rosa_wichuraiana Rw0G001820 Rw1G011230 Rw1G011240 Rw1G027290 Rw2G011670 Rw2G039270 Rw6G018440 Rw6G023120 Rw7G025180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 379
Acc36I ACCTGC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 830
AccI GTMKAC 1 cut(s) 355
AciI CCGC 5 cut(s) 559, 591, 620, 626, 674
AclWI GGATC 1 cut(s) 119
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 30, 237
AcuI CTGAAG 2 cut(s) 335, 520
AcyI GRCGYC 2 cut(s) 380, 677
AdeI CACNNNGTG 1 cut(s) 89
AfaI GTAC 1 cut(s) 602
AfiI CCNNNNNNNGG 2 cut(s) 511, 610
AgsI TTSAA 3 cut(s) 137, 770, 790
AhdI GACNNNNNGTC 1 cut(s) 291
AloI GAACNNNNNNTCC 2 cut(s) 423, 455
AluBI AGCT 4 cut(s) 221, 272, 687, 716
AluI AGCT 4 cut(s) 221, 272, 687, 716
Alw21I GWGCWC 1 cut(s) 525
Alw26I GTCTC 4 cut(s) 299, 513, 541, 812
AlwI GGATC 1 cut(s) 119
Ama87I CYCGRG 2 cut(s) 809, 852
AoxI GGCC 2 cut(s) 409, 747
ApeKI GCWGC 1 cut(s) 881
ApoI RAATTY 2 cut(s) 30, 237
ArsI GACNNNNNNTTYG 4 cut(s) 423, 455, 861, 893
AseI ATTAAT 2 cut(s) 114, 582
AspA2I CCTAGG 1 cut(s) 455
AspS9I GGNCC 2 cut(s) 653, 748
AsuC2I CCSGG 1 cut(s) 363
AsuHPI GGTGA 2 cut(s) 625, 947
AvaI CYCGRG 2 cut(s) 809, 852
AvaII GGWCC 1 cut(s) 653
AvrII CCTAGG 1 cut(s) 455
BanI GGYRCC 1 cut(s) 830
BanII GRGCYC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 672
Bbv12I GWGCWC 1 cut(s) 525
BbvI GCAGC 1 cut(s) 868
BccI CCATC 5 cut(s) 227, 328, 422, 649, 655
BceAI ACGGC 1 cut(s) 604
BciVI GTATCC 1 cut(s) 70
BcnI CCSGG 1 cut(s) 363
BcoDI GTCTC 4 cut(s) 299, 513, 541, 812
BfaI CTAG 6 cut(s) 419, 456, 606, 684, 819, 825
BfuAI ACCTGC 1 cut(s) 157
BfuI GTATCC 1 cut(s) 70
BglII AGATCT 2 cut(s) 183, 821
BisI GCNGC 2 cut(s) 675, 882
BlnI CCTAGG 1 cut(s) 455
BlsI GCNGC 2 cut(s) 676, 883
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 1 cut(s) 653
BmeRI GACNNNNNGTC 1 cut(s) 291
BmeT110I CYCGRG 2 cut(s) 809, 852
BmgT120I GGNCC 2 cut(s) 653, 748
BmiI GGNNCC 2 cut(s) 211, 832
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 181, 310
BpiI GAAGAC 1 cut(s) 672
Bpu10I CCTNAGC 1 cut(s) 445
BpuMI CCSGG 1 cut(s) 363
BsaBI GATNNNNATC 1 cut(s) 150
BsaHI GRCGYC 2 cut(s) 380, 677
BsaI GGTCTC 1 cut(s) 812
BsaJI CCNNGG 4 cut(s) 261, 455, 553, 851
BsaWI WCCGGW 3 cut(s) 511, 772, 946
Bsc4I CCNNNNNNNGG 2 cut(s) 511, 610
Bse1I ACTGG 3 cut(s) 291, 488, 505
Bse3DI GCAATG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 150
BseDI CCNNGG 4 cut(s) 261, 455, 553, 851
BseGI GGATG 5 cut(s) 196, 325, 706, 712, 851
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 2 cut(s) 511, 610
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 2 cut(s) 100, 945
BseNI ACTGG 3 cut(s) 291, 488, 505
BseRI GAGGAG 3 cut(s) 194, 247, 944
BseXI GCAGC 1 cut(s) 868
BshFI GGCC 2 cut(s) 411, 749
BshNI GGYRCC 1 cut(s) 830
BsiHKAI GWGCWC 1 cut(s) 525
BsiHKCI CYCGRG 2 cut(s) 809, 852
BsiSI CCGG 4 cut(s) 363, 512, 773, 947
BslI CCNNNNNNNGG 2 cut(s) 511, 610
BsmAI GTCTC 4 cut(s) 299, 513, 541, 812
BsnI GGCC 2 cut(s) 411, 749
Bso31I GGTCTC 1 cut(s) 812
BsoBI CYCGRG 2 cut(s) 809, 852
Bsp1286I GDGCHC 2 cut(s) 214, 525
Bsp143I GATC 4 cut(s) 124, 183, 821, 939
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 5 cut(s) 559, 591, 620, 626, 674
BspANI GGCC 2 cut(s) 411, 749
BspCNI CTCAG 2 cut(s) 99, 946
BspLI GGNNCC 2 cut(s) 211, 832
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 830
BspTNI GGTCTC 1 cut(s) 812
BsrDI GCAATG 1 cut(s) 93
BsrI ACTGG 3 cut(s) 291, 488, 505
BssECI CCNNGG 4 cut(s) 261, 455, 553, 851
BssMI GATC 4 cut(s) 124, 183, 821, 939
BssNI GRCGYC 2 cut(s) 380, 677
BssT1I CCWWGG 3 cut(s) 261, 455, 553
Bst4CI ACNGT 2 cut(s) 309, 648
Bst6I CTCTTC 2 cut(s) 536, 900
BstACI GRCGYC 2 cut(s) 380, 677
BstDEI CTNAG 5 cut(s) 86, 224, 445, 657, 954
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 5 cut(s) 196, 325, 706, 712, 851
BstKTI GATC 4 cut(s) 127, 186, 824, 942
BstMAI GTCTC 4 cut(s) 299, 513, 541, 812
BstMBI GATC 4 cut(s) 124, 183, 821, 939
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 361
BstV1I GCAGC 1 cut(s) 868
BstV2I GAAGAC 1 cut(s) 672
BstX2I RGATCY 2 cut(s) 183, 821
BstYI RGATCY 2 cut(s) 183, 821
BsuI GTATCC 1 cut(s) 70
BsuRI GGCC 2 cut(s) 411, 749
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 5 cut(s) 196, 325, 706, 712, 851
BtsIMutI CAGTG 1 cut(s) 94
BveI ACCTGC 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 653, 748
CseI GACGC 2 cut(s) 369, 666
Csp6I GTAC 1 cut(s) 601
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CviAII CATG 4 cut(s) 9, 262, 413, 434
CviJI RGCY 9 cut(s) 212, 221, 272, 290, 411, 687, 716, 749, 786
CviKI_1 RGCY 9 cut(s) 212, 221, 272, 290, 411, 687, 716, 749, 786
CviQI GTAC 1 cut(s) 601
DdeI CTNAG 5 cut(s) 86, 224, 445, 657, 954
DpnI GATC 4 cut(s) 126, 185, 823, 941
DpnII GATC 4 cut(s) 124, 183, 821, 939
DraI TTTAAA 1 cut(s) 162
DraIII CACNNNGTG 1 cut(s) 89
DrdI GACNNNNNNGTC 1 cut(s) 379
DriI GACNNNNNGTC 1 cut(s) 291
DseDI GACNNNNNNGTC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 409
Eam1104I CTCTTC 2 cut(s) 536, 900
Eam1105I GACNNNNNGTC 1 cut(s) 291
EarI CTCTTC 2 cut(s) 536, 900
Eco130I CCWWGG 3 cut(s) 261, 455, 553
Eco24I GRGCYC 1 cut(s) 214
Eco31I GGTCTC 1 cut(s) 812
Eco47I GGWCC 1 cut(s) 653
Eco57I CTGAAG 2 cut(s) 335, 520
Eco88I CYCGRG 2 cut(s) 809, 852
EcoT14I CCWWGG 3 cut(s) 261, 455, 553
EcoT38I GRGCYC 1 cut(s) 214
ErhI CCWWGG 3 cut(s) 261, 455, 553
FaeI CATG 4 cut(s) 12, 265, 416, 437
FaiI YATR 9 cut(s) 10, 263, 414, 435, 543, 578, 599, 732, 800
FalI AAGNNNNNCTT 2 cut(s) 650, 682
FatI CATG 4 cut(s) 8, 261, 412, 433
FblI GTMKAC 1 cut(s) 355
Fnu4HI GCNGC 2 cut(s) 675, 882
FokI GGATG 5 cut(s) 203, 332, 713, 719, 858
FriOI GRGCYC 1 cut(s) 214
Fsp4HI GCNGC 2 cut(s) 675, 882
FspBI CTAG 6 cut(s) 419, 456, 606, 684, 819, 825
GluI GCNGC 2 cut(s) 675, 882
HaeIII GGCC 2 cut(s) 411, 749
HapII CCGG 4 cut(s) 363, 512, 773, 947
HgaI GACGC 2 cut(s) 369, 666
Hin1I GRCGYC 2 cut(s) 380, 677
Hin1II CATG 4 cut(s) 12, 265, 416, 437
HinfI GANTC 3 cut(s) 329, 549, 876
HpaII CCGG 4 cut(s) 363, 512, 773, 947
HphI GGTGA 2 cut(s) 625, 947
Hpy166II GTNNAC 3 cut(s) 356, 816, 916
Hpy188I TCNGA 4 cut(s) 37, 72, 480, 904
Hpy188III TCNNGA 3 cut(s) 566, 809, 873
Hpy8I GTNNAC 3 cut(s) 356, 816, 916
HpyAV CCTTC 3 cut(s) 67, 310, 909
HpyCH4III ACNGT 2 cut(s) 309, 648
HpyCH4V TGCA 1 cut(s) 194
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 5 cut(s) 86, 224, 445, 657, 954
Hsp92I GRCGYC 2 cut(s) 380, 677
Hsp92II CATG 4 cut(s) 12, 265, 416, 437
Kzo9I GATC 4 cut(s) 124, 183, 821, 939
LmnI GCTCC 4 cut(s) 209, 477, 528, 775
Lsp1109I GCAGC 1 cut(s) 868
LweI GCATC 2 cut(s) 181, 310
MaeI CTAG 6 cut(s) 419, 456, 606, 684, 819, 825
MaeIII GTNAC 4 cut(s) 89, 169, 613, 949
MalI GATC 4 cut(s) 126, 185, 823, 941
MboI GATC 4 cut(s) 124, 183, 821, 939
MboII GAAGA 7 cut(s) 38, 146, 341, 523, 526, 672, 917
MflI RGATCY 2 cut(s) 183, 821
MhlI GDGCHC 2 cut(s) 214, 525
MluCI AATT 2 cut(s) 30, 237
MlyI GAGTC 3 cut(s) 338, 543, 885
MmeI TCCRAC 2 cut(s) 550, 678
MseI TTAA 7 cut(s) 48, 57, 114, 161, 198, 516, 582
MspI CCGG 4 cut(s) 363, 512, 773, 947
MspR9I CCNGG 1 cut(s) 363
MwoI GCNNNNNNNGC 1 cut(s) 218
NciI CCSGG 1 cut(s) 363
NcoI CCATGG 1 cut(s) 261
NdeII GATC 4 cut(s) 124, 183, 821, 939
NlaIII CATG 4 cut(s) 12, 265, 416, 437
NlaIV GGNNCC 2 cut(s) 211, 832
NmuCI GTSAC 2 cut(s) 89, 613
PaeR7I CTCGAG 1 cut(s) 809
PkrI GCNGC 2 cut(s) 676, 883
PleI GAGTC 3 cut(s) 337, 543, 884
PpsI GAGTC 3 cut(s) 337, 543, 884
PshBI ATTAAT 2 cut(s) 114, 582
PspN4I GGNNCC 2 cut(s) 211, 832
PspPI GGNCC 2 cut(s) 653, 748
PsuI RGATCY 2 cut(s) 183, 821
RsaI GTAC 1 cut(s) 602
RsaNI GTAC 1 cut(s) 601
SaqAI TTAA 7 cut(s) 48, 57, 114, 161, 198, 516, 582
SatI GCNGC 2 cut(s) 675, 882
Sau3AI GATC 4 cut(s) 124, 183, 821, 939
Sau96I GGNCC 2 cut(s) 653, 748
SchI GAGTC 3 cut(s) 338, 543, 885
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 2 cut(s) 214, 525
SetI ASST 8 cut(s) 171, 223, 274, 497, 542, 689, 718, 901
SfaNI GCATC 2 cut(s) 181, 310
Sfr274I CTCGAG 1 cut(s) 809
SinI GGWCC 1 cut(s) 653
SlaI CTCGAG 1 cut(s) 809
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
Sse9I AATT 2 cut(s) 30, 237
SsiI CCGC 5 cut(s) 559, 591, 620, 626, 674
SspMI CTAG 6 cut(s) 419, 456, 606, 684, 819, 825
StyD4I CCNGG 1 cut(s) 361
StyI CCWWGG 3 cut(s) 261, 455, 553
TaaI ACNGT 2 cut(s) 309, 648
TaqI TCGA 3 cut(s) 332, 810, 872
TasI AATT 2 cut(s) 30, 237
TauI GCSGC 1 cut(s) 677
Tru1I TTAA 7 cut(s) 48, 57, 114, 161, 198, 516, 582
Tru9I TTAA 7 cut(s) 48, 57, 114, 161, 198, 516, 582
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 2 cut(s) 89, 613
TseI GCWGC 1 cut(s) 881
Tsp45I GTSAC 2 cut(s) 89, 613
TspDTI ATGAA 1 cut(s) 726
TspGWI ACGGA 2 cut(s) 389, 747
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 653
VspI ATTAAT 2 cut(s) 114, 582
XapI RAATTY 2 cut(s) 30, 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 742
XhoI CTCGAG 1 cut(s) 809
XmaJI CCTAGG 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 355
XspI CTAG 6 cut(s) 419, 456, 606, 684, 819, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.