Belongs to the oxygen-dependent FAD-linked oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 718 |
| AccII | CGCG | 1 cut(s) | 1519 |
| AciI | CCGC | 2 cut(s) | 544, 1337 |
| AclWI | GGATC | 3 cut(s) | 14, 176, 189 |
| AcsI | RAATTY | 2 cut(s) | 536, 748 |
| AcuI | CTGAAG | 3 cut(s) | 343, 666, 1548 |
| AfaI | GTAC | 5 cut(s) | 509, 584, 1203, 1380, 1390 |
| AflIII | ACRYGT | 3 cut(s) | 113, 253, 311 |
| AhlI | ACTAGT | 1 cut(s) | 796 |
| AjnI | CCWGG | 3 cut(s) | 703, 912, 1381 |
| Alw21I | GWGCWC | 2 cut(s) | 37, 924 |
| Alw26I | GTCTC | 2 cut(s) | 844, 1421 |
| Alw44I | GTGCAC | 1 cut(s) | 33 |
| AlwI | GGATC | 3 cut(s) | 14, 176, 189 |
| ApaLI | GTGCAC | 1 cut(s) | 33 |
| ApoI | RAATTY | 2 cut(s) | 536, 748 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 679, 789 |
| AspLEI | GCGC | 1 cut(s) | 1622 |
| AspS9I | GGNCC | 1 cut(s) | 1660 |
| AsuC2I | CCSGG | 1 cut(s) | 1694 |
| AsuHPI | GGTGA | 3 cut(s) | 455, 682, 1544 |
| AvaII | GGWCC | 1 cut(s) | 1660 |
| BaeGI | GKGCMC | 1 cut(s) | 37 |
| BamHI | GGATCC | 1 cut(s) | 181 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1266, 1298 |
| BauI | CACGAG | 1 cut(s) | 1248 |
| Bbv12I | GWGCWC | 2 cut(s) | 37, 924 |
| BccI | CCATC | 6 cut(s) | 53, 280, 1049, 1451, 1600, 1650 |
| BciT130I | CCWGG | 3 cut(s) | 705, 914, 1383 |
| BclI | TGATCA | 2 cut(s) | 405, 832 |
| BcnI | CCSGG | 1 cut(s) | 1694 |
| BcoDI | GTCTC | 2 cut(s) | 844, 1421 |
| BcuI | ACTAGT | 1 cut(s) | 796 |
| BfaI | CTAG | 5 cut(s) | 80, 201, 590, 672, 797 |
| BfmI | CTRYAG | 3 cut(s) | 372, 1593, 1715 |
| Bme1390I | CCNGG | 4 cut(s) | 705, 914, 1383, 1694 |
| Bme18I | GGWCC | 1 cut(s) | 1660 |
| BmgT120I | GGNCC | 1 cut(s) | 1660 |
| BmiI | GGNNCC | 5 cut(s) | 183, 517, 1039, 1661, 1662 |
| BmrFI | CCNGG | 4 cut(s) | 705, 914, 1383, 1694 |
| BmsI | GCATC | 4 cut(s) | 79, 1006, 1586, 1607 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1452, 1484 |
| Bpu10I | CCTNAGC | 1 cut(s) | 627 |
| BpuEI | CTTGAG | 1 cut(s) | 844 |
| BpuMI | CCSGG | 1 cut(s) | 1694 |
| Bsa29I | ATCGAT | 1 cut(s) | 570 |
| BsaBI | GATNNNNATC | 1 cut(s) | 404 |
| BsaJI | CCNNGG | 2 cut(s) | 519, 1778 |
| Bse1I | ACTGG | 1 cut(s) | 13 |
| Bse3DI | GCAATG | 2 cut(s) | 234, 363 |
| Bse8I | GATNNNNATC | 1 cut(s) | 404 |
| BseBI | CCWGG | 3 cut(s) | 705, 914, 1383 |
| BseCI | ATCGAT | 1 cut(s) | 570 |
| BseDI | CCNNGG | 2 cut(s) | 519, 1778 |
| BseGI | GGATG | 5 cut(s) | 862, 1308, 1506, 1598, 1661 |
| BseJI | GATNNNNATC | 1 cut(s) | 404 |
| BseMI | GCAATG | 2 cut(s) | 234, 363 |
| BseNI | ACTGG | 1 cut(s) | 13 |
| BseSI | GKGCMC | 1 cut(s) | 37 |
| Bsh1236I | CGCG | 1 cut(s) | 1519 |
| Bsh1285I | CGRYCG | 1 cut(s) | 544 |
| BshVI | ATCGAT | 1 cut(s) | 570 |
| BsiEI | CGRYCG | 1 cut(s) | 544 |
| BsiHKAI | GWGCWC | 2 cut(s) | 37, 924 |
| BsiSI | CCGG | 1 cut(s) | 1694 |
| BslFI | GGGAC | 3 cut(s) | 495, 1646, 1676 |
| BsmAI | GTCTC | 2 cut(s) | 844, 1421 |
| BsmFI | GGGAC | 3 cut(s) | 495, 1646, 1676 |
| Bsp1286I | GDGCHC | 2 cut(s) | 37, 924 |
| Bsp1407I | TGTACA | 1 cut(s) | 1201 |
| Bsp143I | GATC | 6 cut(s) | 19, 181, 405, 586, 832, 1791 |
| Bsp19I | CCATGG | 1 cut(s) | 1778 |
| BspACI | CCGC | 2 cut(s) | 544, 1337 |
| BspDI | ATCGAT | 1 cut(s) | 570 |
| BspFNI | CGCG | 1 cut(s) | 1519 |
| BspHI | TCATGA | 1 cut(s) | 835 |
| BspLI | GGNNCC | 5 cut(s) | 183, 517, 1039, 1661, 1662 |
| BspMAI | CTGCAG | 2 cut(s) | 376, 1719 |
| BspPI | GGATC | 3 cut(s) | 14, 176, 189 |
| BspQI | GCTCTTC | 1 cut(s) | 1158 |
| BsrDI | GCAATG | 2 cut(s) | 234, 363 |
| BsrGI | TGTACA | 1 cut(s) | 1201 |
| BsrI | ACTGG | 1 cut(s) | 13 |
| BssECI | CCNNGG | 2 cut(s) | 519, 1778 |
| BssMI | GATC | 6 cut(s) | 19, 181, 405, 586, 832, 1791 |
| BssSI | CACGAG | 1 cut(s) | 1248 |
| BssT1I | CCWWGG | 2 cut(s) | 519, 1778 |
| Bst2BI | CACGAG | 1 cut(s) | 1248 |
| Bst2UI | CCWGG | 3 cut(s) | 705, 914, 1383 |
| Bst4CI | ACNGT | 5 cut(s) | 226, 486, 879, 1554, 1640 |
| Bst6I | CTCTTC | 3 cut(s) | 1071, 1158, 1483 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 41 |
| BstAUI | TGTACA | 1 cut(s) | 1201 |
| BstDEI | CTNAG | 3 cut(s) | 129, 605, 627 |
| BstDSI | CCRYGG | 1 cut(s) | 1778 |
| BstF5I | GGATG | 5 cut(s) | 862, 1308, 1506, 1598, 1661 |
| BstFNI | CGCG | 1 cut(s) | 1519 |
| BstHHI | GCGC | 1 cut(s) | 1622 |
| BstKTI | GATC | 6 cut(s) | 22, 184, 408, 589, 835, 1794 |
| BstMAI | GTCTC | 2 cut(s) | 844, 1421 |
| BstMBI | GATC | 6 cut(s) | 19, 181, 405, 586, 832, 1791 |
| BstMCI | CGRYCG | 1 cut(s) | 544 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 41, 245, 374, 638, 1112, 1346, 1619, 1706 |
| BstNI | CCWGG | 3 cut(s) | 705, 914, 1383 |
| BstNSI | RCATGY | 3 cut(s) | 117, 257, 315 |
| BstSCI | CCNGG | 4 cut(s) | 703, 912, 1381, 1692 |
| BstSFI | CTRYAG | 3 cut(s) | 372, 1593, 1715 |
| BstSLI | GKGCMC | 1 cut(s) | 37 |
| BstUI | CGCG | 1 cut(s) | 1519 |
| BstX2I | RGATCY | 2 cut(s) | 19, 181 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 711 |
| BstYI | RGATCY | 2 cut(s) | 19, 181 |
| Bsu15I | ATCGAT | 1 cut(s) | 570 |
| BsuTUI | ATCGAT | 1 cut(s) | 570 |
| BtgI | CCRYGG | 1 cut(s) | 1778 |
| BtgZI | GCGATG | 1 cut(s) | 1500 |
| BtsCI | GGATG | 5 cut(s) | 862, 1308, 1506, 1598, 1661 |
| BtsI | GCAGTG | 3 cut(s) | 351, 924, 1311 |
| BtsIMutI | CAGTG | 5 cut(s) | 222, 329, 351, 924, 1311 |
| CciI | TCATGA | 1 cut(s) | 835 |
| CfoI | GCGC | 1 cut(s) | 1622 |
| Cfr13I | GGNCC | 1 cut(s) | 1660 |
| ClaI | ATCGAT | 1 cut(s) | 570 |
| CseI | GACGC | 1 cut(s) | 893 |
| CsiI | ACCWGGT | 1 cut(s) | 1381 |
| Csp6I | GTAC | 5 cut(s) | 508, 583, 1202, 1379, 1389 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1236, 1271 |
| CviQI | GTAC | 5 cut(s) | 508, 583, 1202, 1379, 1389 |
| DdeI | CTNAG | 3 cut(s) | 129, 605, 627 |
| DpnI | GATC | 6 cut(s) | 21, 183, 407, 588, 834, 1793 |
| DpnII | GATC | 6 cut(s) | 19, 181, 405, 586, 832, 1791 |
| Eam1104I | CTCTTC | 3 cut(s) | 1071, 1158, 1483 |
| EarI | CTCTTC | 3 cut(s) | 1071, 1158, 1483 |
| Eco130I | CCWWGG | 2 cut(s) | 519, 1778 |
| Eco32I | GATATC | 1 cut(s) | 1051 |
| Eco47I | GGWCC | 1 cut(s) | 1660 |
| Eco57I | CTGAAG | 3 cut(s) | 343, 666, 1548 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1660 |
| EcoRI | GAATTC | 1 cut(s) | 536 |
| EcoRII | CCWGG | 3 cut(s) | 703, 912, 1381 |
| EcoRV | GATATC | 1 cut(s) | 1051 |
| EcoT14I | CCWWGG | 2 cut(s) | 519, 1778 |
| EcoT22I | ATGCAT | 1 cut(s) | 1328 |
| ErhI | CCWWGG | 2 cut(s) | 519, 1778 |
| FaqI | GGGAC | 3 cut(s) | 495, 1646, 1676 |
| FauNDI | CATATG | 2 cut(s) | 1098, 1788 |
| FbaI | TGATCA | 2 cut(s) | 405, 832 |
| FblI | GTMKAC | 1 cut(s) | 718 |
| FokI | GGATG | 5 cut(s) | 849, 1315, 1493, 1585, 1668 |
| FspBI | CTAG | 5 cut(s) | 80, 201, 590, 672, 797 |
| GlaI | GCGC | 1 cut(s) | 1621 |
| HapII | CCGG | 1 cut(s) | 1694 |
| HgaI | GACGC | 1 cut(s) | 893 |
| HhaI | GCGC | 1 cut(s) | 1622 |
| Hin6I | GCGC | 1 cut(s) | 1620 |
| HinP1I | GCGC | 1 cut(s) | 1620 |
| HinfI | GANTC | 5 cut(s) | 168, 187, 601, 720, 1572 |
| HpaII | CCGG | 1 cut(s) | 1694 |
| HphI | GGTGA | 3 cut(s) | 455, 682, 1544 |
| Hpy166II | GTNNAC | 4 cut(s) | 35, 317, 719, 1260 |
| Hpy188I | TCNGA | 5 cut(s) | 186, 685, 972, 1396, 1735 |
| Hpy188III | TCNNGA | 9 cut(s) | 80, 172, 409, 513, 823, 836, 870, 938, 1143 |
| Hpy8I | GTNNAC | 4 cut(s) | 35, 317, 719, 1260 |
| Hpy99I | CGWCG | 1 cut(s) | 887 |
| HpyCH4III | ACNGT | 5 cut(s) | 226, 486, 879, 1554, 1640 |
| HpyCH4IV | ACGT | 1 cut(s) | 710 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 41, 245, 374, 638, 1112, 1346, 1619, 1706 |
| HpyF3I | CTNAG | 3 cut(s) | 129, 605, 627 |
| HpySE526I | ACGT | 1 cut(s) | 710 |
| HspAI | GCGC | 1 cut(s) | 1620 |
| KflI | GGGWCCC | 1 cut(s) | 1660 |
| Ksp22I | TGATCA | 2 cut(s) | 405, 832 |
| Kzo9I | GATC | 6 cut(s) | 19, 181, 405, 586, 832, 1791 |
| LguI | GCTCTTC | 1 cut(s) | 1158 |
| LmnI | GCTCC | 2 cut(s) | 245, 1145 |
| LweI | GCATC | 4 cut(s) | 79, 1006, 1586, 1607 |
| MabI | ACCWGGT | 1 cut(s) | 1381 |
| MaeI | CTAG | 5 cut(s) | 80, 201, 590, 672, 797 |
| MaeII | ACGT | 1 cut(s) | 710 |
| MaeIII | GTNAC | 6 cut(s) | 611, 1057, 1281, 1520, 1548, 1640 |
| MalI | GATC | 6 cut(s) | 21, 183, 407, 588, 834, 1793 |
| MboI | GATC | 6 cut(s) | 19, 181, 405, 586, 832, 1791 |
| MboII | GAAGA | 8 cut(s) | 302, 424, 610, 1006, 1088, 1145, 1470, 1737 |
| MfeI | CAATTG | 1 cut(s) | 148 |
| MflI | RGATCY | 2 cut(s) | 19, 181 |
| MhlI | GDGCHC | 2 cut(s) | 37, 924 |
| MlyI | GAGTC | 3 cut(s) | 196, 714, 1566 |
| MmeI | TCCRAC | 1 cut(s) | 510 |
| Mph1103I | ATGCAT | 1 cut(s) | 1328 |
| MroXI | GAANNNNTTC | 2 cut(s) | 679, 789 |
| MseI | TTAA | 3 cut(s) | 108, 927, 944 |
| MslI | CAYNNNNRTG | 3 cut(s) | 840, 1002, 1220 |
| MspA1I | CMGCKG | 4 cut(s) | 371, 377, 1352, 1367 |
| MspI | CCGG | 1 cut(s) | 1694 |
| MspR9I | CCNGG | 4 cut(s) | 705, 914, 1383, 1694 |
| MunI | CAATTG | 1 cut(s) | 148 |
| MvaI | CCWGG | 3 cut(s) | 705, 914, 1383 |
| MvnI | CGCG | 1 cut(s) | 1519 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 41, 245, 374, 638, 1112, 1346, 1619, 1706 |
| NciI | CCSGG | 1 cut(s) | 1694 |
| NcoI | CCATGG | 1 cut(s) | 1778 |
| NdeI | CATATG | 2 cut(s) | 1098, 1788 |
| NdeII | GATC | 6 cut(s) | 19, 181, 405, 586, 832, 1791 |
| NlaIV | GGNNCC | 5 cut(s) | 183, 517, 1039, 1661, 1662 |
| NmuCI | GTSAC | 3 cut(s) | 611, 1057, 1640 |
| NsiI | ATGCAT | 1 cut(s) | 1328 |
| NspI | RCATGY | 3 cut(s) | 117, 257, 315 |
| PagI | TCATGA | 1 cut(s) | 835 |
| PciI | ACATGT | 3 cut(s) | 113, 253, 311 |
| PciSI | GCTCTTC | 1 cut(s) | 1158 |
| PdmI | GAANNNNTTC | 2 cut(s) | 679, 789 |
| PfeI | GAWTC | 2 cut(s) | 168, 601 |
| PflFI | GACNNNGTC | 1 cut(s) | 224 |
| PfoI | TCCNGGA | 1 cut(s) | 703 |
| PleI | GAGTC | 3 cut(s) | 195, 714, 1566 |
| PpsI | GAGTC | 3 cut(s) | 195, 714, 1566 |
| PpuMI | RGGWCCY | 1 cut(s) | 1660 |
| PscI | ACATGT | 3 cut(s) | 113, 253, 311 |
| Psp5II | RGGWCCY | 1 cut(s) | 1660 |
| Psp6I | CCWGG | 3 cut(s) | 703, 912, 1381 |
| PspGI | CCWGG | 3 cut(s) | 703, 912, 1381 |
| PspN4I | GGNNCC | 5 cut(s) | 183, 517, 1039, 1661, 1662 |
| PspPI | GGNCC | 1 cut(s) | 1660 |
| PspPPI | RGGWCCY | 1 cut(s) | 1660 |
| PstI | CTGCAG | 2 cut(s) | 376, 1719 |
| PsuI | RGATCY | 2 cut(s) | 19, 181 |
| PsyI | GACNNNGTC | 1 cut(s) | 224 |
| PvuII | CAGCTG | 4 cut(s) | 371, 377, 1352, 1367 |
| RsaI | GTAC | 5 cut(s) | 509, 584, 1203, 1380, 1390 |
| RsaNI | GTAC | 5 cut(s) | 508, 583, 1202, 1379, 1389 |
| RseI | CAYNNNNRTG | 3 cut(s) | 840, 1002, 1220 |
| SapI | GCTCTTC | 1 cut(s) | 1158 |
| SaqAI | TTAA | 3 cut(s) | 108, 927, 944 |
| Sau3AI | GATC | 6 cut(s) | 19, 181, 405, 586, 832, 1791 |
| Sau96I | GGNCC | 1 cut(s) | 1660 |
| SchI | GAGTC | 3 cut(s) | 196, 714, 1566 |
| ScrFI | CCNGG | 4 cut(s) | 705, 914, 1383, 1694 |
| SduI | GDGCHC | 2 cut(s) | 37, 924 |
| SexAI | ACCWGGT | 1 cut(s) | 1381 |
| SfaNI | GCATC | 4 cut(s) | 79, 1006, 1586, 1607 |
| SfcI | CTRYAG | 3 cut(s) | 372, 1593, 1715 |
| SinI | GGWCC | 1 cut(s) | 1660 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 840, 1002, 1220 |
| SmlI | CTYRAG | 1 cut(s) | 823 |
| SmoI | CTYRAG | 1 cut(s) | 823 |
| SpeI | ACTAGT | 1 cut(s) | 796 |
| SsiI | CCGC | 2 cut(s) | 544, 1337 |
| SspMI | CTAG | 5 cut(s) | 80, 201, 590, 672, 797 |
| StyD4I | CCNGG | 4 cut(s) | 703, 912, 1381, 1692 |
| StyI | CCWWGG | 2 cut(s) | 519, 1778 |
| TaaI | ACNGT | 5 cut(s) | 226, 486, 879, 1554, 1640 |
| TaiI | ACGT | 1 cut(s) | 713 |
| TaqI | TCGA | 7 cut(s) | 59, 279, 436, 540, 570, 882, 1794 |
| TatI | WGTACW | 2 cut(s) | 507, 1201 |
| TauI | GCSGC | 1 cut(s) | 1340 |
| TfiI | GAWTC | 2 cut(s) | 168, 601 |
| Tru1I | TTAA | 3 cut(s) | 108, 927, 944 |
| Tru9I | TTAA | 3 cut(s) | 108, 927, 944 |
| TscAI | CASTG | 5 cut(s) | 229, 336, 358, 924, 1318 |
| TseFI | GTSAC | 3 cut(s) | 611, 1057, 1640 |
| Tsp45I | GTSAC | 3 cut(s) | 611, 1057, 1640 |
| TspDTI | ATGAA | 9 cut(s) | 356, 798, 1089, 1238, 1287, 1311, 1319, 1495, 1576 |
| TspRI | CASTG | 5 cut(s) | 229, 336, 358, 924, 1318 |
| Tth111I | GACNNNGTC | 1 cut(s) | 224 |
| VneI | GTGCAC | 1 cut(s) | 33 |
| VpaK11BI | GGWCC | 1 cut(s) | 1660 |
| XapI | RAATTY | 2 cut(s) | 536, 748 |
| XbaI | TCTAGA | 1 cut(s) | 79 |
| XceI | RCATGY | 3 cut(s) | 117, 257, 315 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 526 |
| XmiI | GTMKAC | 1 cut(s) | 718 |
| XmnI | GAANNNNTTC | 2 cut(s) | 679, 789 |
| XspI | CTAG | 5 cut(s) | 80, 201, 590, 672, 797 |
| Zsp2I | ATGCAT | 1 cut(s) | 1328 |