Rw6G023120

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000172
Physical Location & Seq
Reverse (-)
45310626 .. 45313946
3321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G023120.1

Sequence Viewer

Length: 837 bp
ATGTGGAGGCTGGCTGGGACAGTAGAGGTCCAACCAAGAGGTGATCGTTTTCTCTTTACGTTCACCCATGAACGTGATGTTGCTCGTGTGAAGAAGGGGGGTCCCTGGGGCTTTCAGCGAGCCATGCTCCTCCTGAATGACTATGATGGGTTTTCAGAGATCTCCGAGGTGAAGCTAGATTTTGTGTGGATATGGATTATGCTGCAAGGTATTCCCCCAGGTATTCTGACTAAGCCTACGGTCAGGTTGGTGGGCAGGACAATTAGAGAAGTGCTGGAGGTAGATCGTGGTGCGTTGCAGTGGGGAGAGGCCCGTGTCCGGTTTGTGTTACTTATCAATGAGCCGGTGCGATTGGATCGAAGGGTTAGGGTTTCTCCTACTGATGTCCTAACACTTCGTTTTAGATACGAACGTCTGTTGGGGCGCTGCCGGATCTGTACTATGCTTAATCACAGTAGGCAACGTTGTACGAGGGAGACAGAGCCAGCTTTAGAACTGGAAGCGGAGGAGGGGACGCTGTCGGTTGCTGGGCCGATAGTTCTTGCCCTAGTTTTTAGGGCGAGTGCTCAGCCTACTGGTTCACCTTCCTTGCTATCTGCTTTCAGGATCCCTAATATGCTGAAGAAACGATCGGTGCAGATTCGACCGCTGCCTGAGATGGTGCTGACGTCGGGGACTGAAGGGACCTCTTCTCAACAGCTTGTGGGGATGCATCGTCCACGGGAGGAAGAGACCCAGGGAGCGGGGAAGCGTGCCAAACACGGTTTGGATCTTGTCTCAGTTGATTTGCAGCCGGAAAATCTCGGTTTGGAGCTCTCTTCCGAGGGACCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

278

Amino Acids

31.37

Weight (kDa)

9.54

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 112 - 157 3.9e-11 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59650 AT3G59650
fragaria_vesca FvH4_1g19981 FvH4_1g22221 FvH4_2g13530 FvH4_2g13791 FvH4_2g14332 FvH4_2g14911 FvH4_2g16332 FvH4_3g10980 FvH4_3g11141 FvH4_3g14171 FvH4_3g26311 FvH4_3g28254 FvH4_3g29741 FvH4_4g05261 FvH4_4g12002 FvH4_4g13371 FvH4_4g15541 FvH4_4g18531 FvH4_5g02661 FvH4_5g02662 FvH4_5g16791 FvH4_5g29700 FvH4_5g35881 FvH4_5g37061 FvH4_6g01571 FvH4_6g12761 FvH4_6g14961 FvH4_6g20381 FvH4_6g22001 FvH4_6g23673 FvH4_6g23960 FvH4_6g28051 FvH4_6g31094 FvH4_6g36541 FvH4_7g00911 FvH4_7g06200 FvH4_7g06200 FvH4_7g09551
malus_domestica MD08G1230600.v1.1
prunus_persica Prupe.1G206900_v2.0.a1 Prupe.1G206900_v2.0.a1 Prupe.1G206900_v2.0.a1
pyrus_communis pycom08g20050 pycom15g37590
rosa_chinensis RchiOBHm_Chr1g0333831 RchiOBHm_Chr1g0344871 RchiOBHm_Chr3g0473241 RchiOBHm_Chr5g0018871 RchiOBHm_Chr6g0265791
rosa_laevigata RLG00000005720 RLG00000012718 RLG00000027174 RLG00000029225 RLG00000029527
rosa_multiflora Rmu_co8002714.1_g000001 Rmu_co8072270.1_g000001 Rmu_co8095594.1_g000001 Rmu_co8308871.1_g000001 Rmu_co8398699.1_g000001 Rmu_co8404287.1_g000001 Rmu_sc0000035.1_g000010 Rmu_sc0000133.1_g000007 Rmu_sc0000253.1_g000023 Rmu_sc0000308.1_g000010 Rmu_sc0000309.1_g000030 Rmu_sc0000515.1_g000015 Rmu_sc0000865.1_g000001 Rmu_sc0000889.1_g000005 Rmu_sc0001262.1_g000011 Rmu_sc0001518.1_g000013 Rmu_sc0001533.1_g000006 Rmu_sc0001547.1_g000007 Rmu_sc0001826.1_g000013 Rmu_sc0002060.1_g000017 Rmu_sc0002129.1_g000005 Rmu_sc0002449.1_g000016 Rmu_sc0002460.1_g000036 Rmu_sc0002475.1_g000014 Rmu_sc0002553.1_g000017 Rmu_sc0002759.1_g000033 Rmu_sc0002918.1_g000009 Rmu_sc0002955.1_g000020 Rmu_sc0003094.1_g000010 Rmu_sc0003669.1_g000009 Rmu_sc0004109.1_g000008 Rmu_sc0004350.1_g000007 Rmu_sc0004542.1_g000018 Rmu_sc0004967.1_g000005 Rmu_sc0005026.1_g000005 Rmu_sc0005369.1_g000013 Rmu_sc0005400.1_g000025 Rmu_sc0005420.1_g000006 Rmu_sc0005467.1_g000013 Rmu_sc0005497.1_g000003 Rmu_sc0005607.1_g000023 Rmu_sc0005949.1_g000008 Rmu_sc0005970.1_g000002 Rmu_sc0006452.1_g000001 Rmu_sc0006720.1_g000008 Rmu_sc0006867.1_g000004 Rmu_sc0007177.1_g000006 Rmu_sc0007199.1_g000011 Rmu_sc0007783.1_g000004 Rmu_sc0007885.1_g000006 Rmu_sc0008619.1_g000001 Rmu_sc0008709.1_g000004 Rmu_sc0008709.1_g000005 Rmu_sc0009185.1_g000029 Rmu_sc0010244.1_g000010 Rmu_sc0011610.1_g000005 Rmu_sc0011680.1_g000013 Rmu_sc0012075.1_g000003 Rmu_sc0012278.1_g000001 Rmu_sc0012628.1_g000001 Rmu_sc0018485.1_g000006 Rmu_sc0019960.1_g000001 Rmu_sc0020177.1_g000002 Rmu_sc0020656.1_g000001 Rmu_sc0021333.1_g000003 Rmu_sc0023170.1_g000002 Rmu_sc0023989.1_g000005 Rmu_sc0025259.1_g000001 Rmu_sc0025350.1_g000013 Rmu_sc0029678.1_g000002 Rmu_sc0035939.1_g000001 Rmu_sc0039260.1_g000001 Rmu_sc0039886.1_g000001 Rmu_ssc0000323.1_g000030 Rmu_ssc0000333.1_g000005 Rmu_ssc0000471.1_g000017 Rmu_ssc0000480.1_g000025
rosa_roxburghii Rroxscaffold_1G00006060 Rroxscaffold_1G00048570 Rroxscaffold_1G00057650 Rroxscaffold_1G00072720 Rroxscaffold_4G00281190 Rroxscaffold_4G00317160 Rroxscaffold_6G00411570
rosa_rugosa Rorug01G0004800 Rorug01G0112100 Rorug01G0206100 Rorug02G0324000 Rorug05G0305600 Rorug05G0326800 Rorug06G0018900 Rorug06G0167000 Rorug07G0031200 Rorug07G0071900
rosa_samantha Rh1AG134700 Rh1BG103000 Rh1CG007300 Rh1CG128100 Rh1DG140100 Rh2DG579500 Rh3DG199000 Rh4BG252500 Rh5AG374900 Rh5BG146800 Rh5BG151500 Rh5DG399700
rosa_wichuraiana Rw0G001820 Rw1G011230 Rw1G011240 Rw1G027290 Rw2G011670 Rw2G039270 Rw6G018440 Rw6G023120 Rw7G025180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 671
AccBSI CCGCTC 1 cut(s) 743
AciI CCGC 3 cut(s) 503, 647, 743
AclI AACGTT 1 cut(s) 463
AclWI GGATC 5 cut(s) 363, 440, 601, 614, 777
AcuI CTGAAG 2 cut(s) 641, 699
AcyI GRCGYC 1 cut(s) 668
AfaI GTAC 2 cut(s) 439, 469
AfiI CCNNNNNNNGG 2 cut(s) 318, 742
AjnI CCWGG 3 cut(s) 104, 217, 735
AluBI AGCT 4 cut(s) 175, 488, 700, 814
AluI AGCT 4 cut(s) 175, 488, 700, 814
Alw21I GWGCWC 2 cut(s) 568, 816
Alw26I GTCTC 3 cut(s) 470, 725, 781
AlwI GGATC 5 cut(s) 363, 440, 601, 614, 777
AoxI GGCC 2 cut(s) 309, 530
ApeKI GCWGC 4 cut(s) 202, 426, 649, 790
AspLEI GCGC 1 cut(s) 426
AspS9I GGNCC 6 cut(s) 28, 101, 310, 530, 684, 827
AsuHPI GGTGA 4 cut(s) 53, 55, 181, 573
AvaII GGWCC 4 cut(s) 28, 101, 684, 827
BaeI ACNNNNGTAYC 2 cut(s) 397, 430
BamHI GGATCC 1 cut(s) 606
BanII GRGCYC 1 cut(s) 816
BauI CACGAG 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 568, 816
BbvI GCAGC 4 cut(s) 189, 413, 636, 802
BccI CCATC 2 cut(s) 140, 652
BciT130I CCWGG 3 cut(s) 106, 219, 737
BcoDI GTCTC 3 cut(s) 470, 725, 781
BfaI CTAG 2 cut(s) 176, 548
BfoI RGCGCY 1 cut(s) 427
BglII AGATCT 1 cut(s) 159
BisI GCNGC 4 cut(s) 203, 427, 650, 791
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 4 cut(s) 204, 428, 651, 792
Bme1390I CCNGG 3 cut(s) 106, 219, 737
Bme18I GGWCC 4 cut(s) 28, 101, 684, 827
BmgT120I GGNCC 6 cut(s) 28, 101, 310, 530, 684, 827
BmiI GGNNCC 5 cut(s) 102, 103, 608, 685, 828
BmrFI CCNGG 3 cut(s) 106, 219, 737
BmsI GCATC 2 cut(s) 699, 721
BplI GAGNNNNNCTC 2 cut(s) 111, 143
BpmI CTGGAG 1 cut(s) 296
Bpu1102I GCTNAGC 1 cut(s) 567
BsaHI GRCGYC 1 cut(s) 668
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 8 cut(s) 104, 105, 165, 217, 719, 735, 736, 822
BsaWI WCCGGW 1 cut(s) 318
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 742
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 2 cut(s) 501, 580
BseBI CCWGG 3 cut(s) 106, 219, 737
BseDI CCNNGG 8 cut(s) 104, 105, 165, 217, 719, 735, 736, 822
BseGI GGATG 1 cut(s) 714
BseLI CCNNNNNNNGG 2 cut(s) 318, 742
BseMII CTCAG 3 cut(s) 581, 645, 792
BseNI ACTGG 2 cut(s) 501, 580
BseRI GAGGAG 2 cut(s) 119, 521
BseXI GCAGC 4 cut(s) 189, 413, 636, 802
BseYI CCCAGC 2 cut(s) 14, 527
BsgI GTGCAG 1 cut(s) 656
Bsh1285I CGRYCG 2 cut(s) 632, 647
BshFI GGCC 2 cut(s) 311, 532
BsiEI CGRYCG 2 cut(s) 632, 647
BsiHKAI GWGCWC 2 cut(s) 568, 816
BsiSI CCGG 4 cut(s) 319, 344, 430, 794
BslFI GGGAC 5 cut(s) 31, 87, 526, 688, 697
BslI CCNNNNNNNGG 2 cut(s) 318, 742
BsmAI GTCTC 3 cut(s) 470, 725, 781
BsmFI GGGAC 5 cut(s) 31, 87, 526, 688, 697
BsnI GGCC 2 cut(s) 311, 532
Bso31I GGTCTC 1 cut(s) 725
Bsp1286I GDGCHC 2 cut(s) 568, 816
Bsp143I GATC 8 cut(s) 43, 159, 283, 355, 432, 606, 629, 769
Bsp1720I GCTNAGC 1 cut(s) 567
BspACI CCGC 3 cut(s) 503, 647, 743
BspANI GGCC 2 cut(s) 311, 532
BspCNI CTCAG 3 cut(s) 580, 646, 791
BspLI GGNNCC 5 cut(s) 102, 103, 608, 685, 828
BspPI GGATC 5 cut(s) 363, 440, 601, 614, 777
BspTNI GGTCTC 1 cut(s) 725
BsrBI CCGCTC 1 cut(s) 743
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 2 cut(s) 501, 580
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 8 cut(s) 104, 105, 165, 217, 719, 735, 736, 822
BssMI GATC 8 cut(s) 43, 159, 283, 355, 432, 606, 629, 769
BssNI GRCGYC 1 cut(s) 668
BssSI CACGAG 1 cut(s) 84
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 3 cut(s) 106, 219, 737
Bst4CI ACNGT 4 cut(s) 22, 241, 455, 764
Bst6I CTCTTC 3 cut(s) 694, 723, 823
BstACI GRCGYC 1 cut(s) 668
BstC8I GCNNGC 4 cut(s) 12, 120, 486, 753
BstDEI CTNAG 4 cut(s) 231, 567, 654, 778
BstDSI CCRYGG 1 cut(s) 719
BstF5I GGATG 1 cut(s) 714
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 8 cut(s) 46, 162, 286, 358, 435, 609, 632, 772
BstMAI GTCTC 3 cut(s) 470, 725, 781
BstMBI GATC 8 cut(s) 43, 159, 283, 355, 432, 606, 629, 769
BstMCI CGRYCG 2 cut(s) 632, 647
BstMWI GCNNNNNNNGC 1 cut(s) 124
BstNI CCWGG 3 cut(s) 106, 219, 737
BstSCI CCNGG 3 cut(s) 104, 217, 735
BstV1I GCAGC 4 cut(s) 189, 413, 636, 802
BstX2I RGATCY 4 cut(s) 159, 432, 606, 769
BstYI RGATCY 4 cut(s) 159, 432, 606, 769
BsuRI GGCC 2 cut(s) 311, 532
BtgI CCRYGG 1 cut(s) 719
BtsCI GGATG 1 cut(s) 714
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 1 cut(s) 305
Cac8I GCNNGC 4 cut(s) 12, 120, 486, 753
CfoI GCGC 1 cut(s) 426
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 6 cut(s) 28, 101, 310, 530, 684, 827
CseI GACGC 1 cut(s) 523
Csp6I GTAC 2 cut(s) 438, 468
CviAII CATG 2 cut(s) 68, 124
CviQI GTAC 2 cut(s) 438, 468
DdeI CTNAG 4 cut(s) 231, 567, 654, 778
DpnI GATC 8 cut(s) 45, 161, 285, 357, 434, 608, 631, 771
DpnII GATC 8 cut(s) 43, 159, 283, 355, 432, 606, 629, 769
Eam1104I CTCTTC 3 cut(s) 694, 723, 823
EarI CTCTTC 3 cut(s) 694, 723, 823
Ecl136II GAGCTC 1 cut(s) 814
Eco24I GRGCYC 1 cut(s) 816
Eco31I GGTCTC 1 cut(s) 725
Eco47I GGWCC 4 cut(s) 28, 101, 684, 827
Eco53kI GAGCTC 1 cut(s) 814
Eco57I CTGAAG 2 cut(s) 641, 699
EcoICRI GAGCTC 1 cut(s) 814
EcoO109I RGGNCCY 2 cut(s) 101, 684
EcoRII CCWGG 3 cut(s) 104, 217, 735
EcoT22I ATGCAT 1 cut(s) 714
EcoT38I GRGCYC 1 cut(s) 816
FaeI CATG 2 cut(s) 71, 127
FaiI YATR 7 cut(s) 69, 125, 144, 193, 200, 443, 617
FaqI GGGAC 5 cut(s) 31, 87, 526, 688, 697
FatI CATG 2 cut(s) 67, 123
FauI CCCGC 1 cut(s) 736
Fnu4HI GCNGC 4 cut(s) 203, 427, 650, 791
FokI GGATG 1 cut(s) 721
FriOI GRGCYC 1 cut(s) 816
Fsp4HI GCNGC 4 cut(s) 203, 427, 650, 791
FspBI CTAG 2 cut(s) 176, 548
GlaI GCGC 1 cut(s) 425
GluI GCNGC 4 cut(s) 203, 427, 650, 791
GsaI CCCAGC 2 cut(s) 18, 531
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 2 cut(s) 311, 532
HapII CCGG 4 cut(s) 319, 344, 430, 794
HgaI GACGC 1 cut(s) 523
HhaI GCGC 1 cut(s) 426
Hin1I GRCGYC 1 cut(s) 668
Hin1II CATG 2 cut(s) 71, 127
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HinfI GANTC 1 cut(s) 640
HpaII CCGG 4 cut(s) 319, 344, 430, 794
HphI GGTGA 4 cut(s) 53, 55, 181, 573
Hpy166II GTNNAC 3 cut(s) 63, 581, 719
Hpy188I TCNGA 4 cut(s) 157, 166, 228, 823
Hpy188III TCNNGA 2 cut(s) 133, 604
Hpy8I GTNNAC 3 cut(s) 63, 581, 719
Hpy99I CGWCG 1 cut(s) 673
HpyAV CCTTC 4 cut(s) 88, 354, 594, 674
HpyCH4III ACNGT 4 cut(s) 22, 241, 455, 764
HpyCH4IV ACGT 5 cut(s) 59, 73, 412, 463, 668
HpyCH4V TGCA 5 cut(s) 205, 298, 637, 712, 790
HpyF10VI GCNNNNNNNGC 1 cut(s) 124
HpyF3I CTNAG 4 cut(s) 231, 567, 654, 778
HpySE526I ACGT 5 cut(s) 59, 73, 412, 463, 668
Hsp92I GRCGYC 1 cut(s) 668
Hsp92II CATG 2 cut(s) 71, 127
HspAI GCGC 1 cut(s) 424
KflI GGGWCCC 1 cut(s) 101
Kzo9I GATC 8 cut(s) 43, 159, 283, 355, 432, 606, 629, 769
LmnI GCTCC 3 cut(s) 132, 740, 811
Lsp1109I GCAGC 4 cut(s) 189, 413, 636, 802
LweI GCATC 2 cut(s) 699, 721
MaeI CTAG 2 cut(s) 176, 548
MaeII ACGT 5 cut(s) 59, 73, 412, 463, 668
MaeIII GTNAC 1 cut(s) 327
MalI GATC 8 cut(s) 45, 161, 285, 357, 434, 608, 631, 771
MbiI CCGCTC 1 cut(s) 743
MboI GATC 8 cut(s) 43, 159, 283, 355, 432, 606, 629, 769
MboII GAAGA 5 cut(s) 103, 634, 681, 740, 810
MflI RGATCY 4 cut(s) 159, 432, 606, 769
MhlI GDGCHC 2 cut(s) 568, 816
MluCI AATT 2 cut(s) 261, 832
MmeI TCCRAC 1 cut(s) 55
Mph1103I ATGCAT 1 cut(s) 714
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 649
MspI CCGG 4 cut(s) 319, 344, 430, 794
MspR9I CCNGG 3 cut(s) 106, 219, 737
MvaI CCWGG 3 cut(s) 106, 219, 737
MwoI GCNNNNNNNGC 1 cut(s) 124
NdeII GATC 8 cut(s) 43, 159, 283, 355, 432, 606, 629, 769
NlaIII CATG 2 cut(s) 71, 127
NlaIV GGNNCC 5 cut(s) 102, 103, 608, 685, 828
NsiI ATGCAT 1 cut(s) 714
PasI CCCWGGG 2 cut(s) 105, 736
PfeI GAWTC 1 cut(s) 640
PflFI GACNNNGTC 1 cut(s) 517
PkrI GCNGC 4 cut(s) 204, 428, 651, 792
Ple19I CGATCG 1 cut(s) 632
PpuMI RGGWCCY 2 cut(s) 101, 684
Psp124BI GAGCTC 1 cut(s) 816
Psp1406I AACGTT 1 cut(s) 463
Psp5II RGGWCCY 2 cut(s) 101, 684
Psp6I CCWGG 3 cut(s) 104, 217, 735
PspFI CCCAGC 2 cut(s) 14, 527
PspGI CCWGG 3 cut(s) 104, 217, 735
PspN4I GGNNCC 5 cut(s) 102, 103, 608, 685, 828
PspPI GGNCC 6 cut(s) 28, 101, 310, 530, 684, 827
PspPPI RGGWCCY 2 cut(s) 101, 684
PsuI RGATCY 4 cut(s) 159, 432, 606, 769
PsyI GACNNNGTC 1 cut(s) 517
PvuI CGATCG 1 cut(s) 632
RsaI GTAC 2 cut(s) 439, 469
RsaNI GTAC 2 cut(s) 438, 468
RseI CAYNNNNRTG 1 cut(s) 72
SacI GAGCTC 1 cut(s) 816
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 4 cut(s) 203, 427, 650, 791
Sau3AI GATC 8 cut(s) 43, 159, 283, 355, 432, 606, 629, 769
Sau96I GGNCC 6 cut(s) 28, 101, 310, 530, 684, 827
ScrFI CCNGG 3 cut(s) 106, 219, 737
SduI GDGCHC 2 cut(s) 568, 816
SfaNI GCATC 2 cut(s) 699, 721
SinI GGWCC 4 cut(s) 28, 101, 684, 827
SmiMI CAYNNNNRTG 1 cut(s) 72
Sse9I AATT 2 cut(s) 261, 832
SsiI CCGC 3 cut(s) 503, 647, 743
SspMI CTAG 2 cut(s) 176, 548
SstI GAGCTC 1 cut(s) 816
StyD4I CCNGG 3 cut(s) 104, 217, 735
TaaI ACNGT 4 cut(s) 22, 241, 455, 764
TaiI ACGT 5 cut(s) 62, 76, 415, 466, 671
TaqI TCGA 2 cut(s) 358, 643
TasI AATT 2 cut(s) 261, 832
TatI WGTACW 1 cut(s) 437
TfiI GAWTC 1 cut(s) 640
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 1 cut(s) 305
TseI GCWGC 4 cut(s) 202, 426, 649, 790
TspDTI ATGAA 1 cut(s) 84
TspRI CASTG 1 cut(s) 305
Tth111I GACNNNGTC 1 cut(s) 517
VpaK11BI GGWCC 4 cut(s) 28, 101, 684, 827
XcmI CCANNNNNNNNNTGG 1 cut(s) 763
XspI CTAG 2 cut(s) 176, 548
ZraI GACGTC 1 cut(s) 669
Zsp2I ATGCAT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.