Rw6G018440

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000172
Physical Location & Seq
Reverse (-)
36629970 .. 36630530
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G018440.1

Sequence Viewer

Length: 561 bp
ATGACTGCCAGGCTAGCGACCAAGTTGTCGCTCAGAGAGGGTGAAGAACAGGCGGACCTGGGGAACCTTAGGGTTCCGGGAAAGGAATTTCTTGCTCGCCGGTTTTACTTGGTGGGGAAACTCAACACTTGTAGGGCGGTGGTTTTGGATTCGTTCCGGAGTGTGGTTCGATCCATGTGGAGGCTGACGAGGACGGTGGAGGTCCAACCCTGGGGTGATCAATTTCTTTTTACGTTCACTCATGAACGTGATGTTGCGAGTGTGAAAAAGGGAGGTCCATGGGGATTTCAACGGGCTATGATTTTGCTGAATGACTATGATGGCTTTTCGGAGATTGCTGAGGTGAAGCTCAATTTCGTCTGGATCTGGGTTGTGCTTCAGGGCATCCCACCGGGTATCTTGACTGAACCCACAGTCAGTTTGGTGGGAGGGACGATTGGAGAGGTCTTGGAAGTGGATCGTTTGGCTCTTCGTCATGGGGAAGCTCGGGTCAGGCTTGTTCTCTCCATCAATGATCCGGTGCGTCTGGATCGAAAGGTTAGGGTTTCTCCCACTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

186

Amino Acids

21.05

Weight (kDa)

9.71

Isoelectric Point (pI)

31.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 35 - 107 5.2e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59650 AT3G59650
fragaria_vesca FvH4_1g19981 FvH4_1g22221 FvH4_2g13530 FvH4_2g13791 FvH4_2g14332 FvH4_2g14911 FvH4_2g16332 FvH4_3g10980 FvH4_3g11141 FvH4_3g14171 FvH4_3g26311 FvH4_3g28254 FvH4_3g29741 FvH4_4g05261 FvH4_4g12002 FvH4_4g13371 FvH4_4g15541 FvH4_4g18531 FvH4_5g02661 FvH4_5g02662 FvH4_5g16791 FvH4_5g29700 FvH4_5g35881 FvH4_5g37061 FvH4_6g01571 FvH4_6g12761 FvH4_6g14961 FvH4_6g20381 FvH4_6g22001 FvH4_6g23673 FvH4_6g23960 FvH4_6g28051 FvH4_6g31094 FvH4_6g36541 FvH4_7g00911 FvH4_7g06200 FvH4_7g06200 FvH4_7g09551
malus_domestica MD08G1230600.v1.1
prunus_persica Prupe.1G206900_v2.0.a1 Prupe.1G206900_v2.0.a1 Prupe.1G206900_v2.0.a1
pyrus_communis pycom08g20050 pycom15g37590
rosa_chinensis RchiOBHm_Chr1g0333831 RchiOBHm_Chr1g0344871 RchiOBHm_Chr3g0473241 RchiOBHm_Chr5g0018871 RchiOBHm_Chr6g0265791
rosa_laevigata RLG00000005720 RLG00000012718 RLG00000027174 RLG00000029225 RLG00000029527
rosa_multiflora Rmu_co8002714.1_g000001 Rmu_co8072270.1_g000001 Rmu_co8095594.1_g000001 Rmu_co8308871.1_g000001 Rmu_co8398699.1_g000001 Rmu_co8404287.1_g000001 Rmu_sc0000035.1_g000010 Rmu_sc0000133.1_g000007 Rmu_sc0000253.1_g000023 Rmu_sc0000308.1_g000010 Rmu_sc0000309.1_g000030 Rmu_sc0000515.1_g000015 Rmu_sc0000865.1_g000001 Rmu_sc0000889.1_g000005 Rmu_sc0001262.1_g000011 Rmu_sc0001518.1_g000013 Rmu_sc0001533.1_g000006 Rmu_sc0001547.1_g000007 Rmu_sc0001826.1_g000013 Rmu_sc0002060.1_g000017 Rmu_sc0002129.1_g000005 Rmu_sc0002449.1_g000016 Rmu_sc0002460.1_g000036 Rmu_sc0002475.1_g000014 Rmu_sc0002553.1_g000017 Rmu_sc0002759.1_g000033 Rmu_sc0002918.1_g000009 Rmu_sc0002955.1_g000020 Rmu_sc0003094.1_g000010 Rmu_sc0003669.1_g000009 Rmu_sc0004109.1_g000008 Rmu_sc0004350.1_g000007 Rmu_sc0004542.1_g000018 Rmu_sc0004967.1_g000005 Rmu_sc0005026.1_g000005 Rmu_sc0005369.1_g000013 Rmu_sc0005400.1_g000025 Rmu_sc0005420.1_g000006 Rmu_sc0005467.1_g000013 Rmu_sc0005497.1_g000003 Rmu_sc0005607.1_g000023 Rmu_sc0005949.1_g000008 Rmu_sc0005970.1_g000002 Rmu_sc0006452.1_g000001 Rmu_sc0006720.1_g000008 Rmu_sc0006867.1_g000004 Rmu_sc0007177.1_g000006 Rmu_sc0007199.1_g000011 Rmu_sc0007783.1_g000004 Rmu_sc0007885.1_g000006 Rmu_sc0008619.1_g000001 Rmu_sc0008709.1_g000004 Rmu_sc0008709.1_g000005 Rmu_sc0009185.1_g000029 Rmu_sc0010244.1_g000010 Rmu_sc0011610.1_g000005 Rmu_sc0011680.1_g000013 Rmu_sc0012075.1_g000003 Rmu_sc0012278.1_g000001 Rmu_sc0012628.1_g000001 Rmu_sc0018485.1_g000006 Rmu_sc0019960.1_g000001 Rmu_sc0020177.1_g000002 Rmu_sc0020656.1_g000001 Rmu_sc0021333.1_g000003 Rmu_sc0023170.1_g000002 Rmu_sc0023989.1_g000005 Rmu_sc0025259.1_g000001 Rmu_sc0025350.1_g000013 Rmu_sc0029678.1_g000002 Rmu_sc0035939.1_g000001 Rmu_sc0039260.1_g000001 Rmu_sc0039886.1_g000001 Rmu_ssc0000323.1_g000030 Rmu_ssc0000333.1_g000005 Rmu_ssc0000471.1_g000017 Rmu_ssc0000480.1_g000025
rosa_roxburghii Rroxscaffold_1G00006060 Rroxscaffold_1G00048570 Rroxscaffold_1G00057650 Rroxscaffold_1G00072720 Rroxscaffold_4G00281190 Rroxscaffold_4G00317160 Rroxscaffold_6G00411570
rosa_rugosa Rorug01G0004800 Rorug01G0112100 Rorug01G0206100 Rorug02G0324000 Rorug05G0305600 Rorug05G0326800 Rorug06G0018900 Rorug06G0167000 Rorug07G0031200 Rorug07G0071900
rosa_samantha Rh1AG134700 Rh1BG103000 Rh1CG007300 Rh1CG128100 Rh1DG140100 Rh2DG579500 Rh3DG199000 Rh4BG252500 Rh5AG374900 Rh5BG146800 Rh5BG151500 Rh5DG399700
rosa_wichuraiana Rw0G001820 Rw1G011230 Rw1G011240 Rw1G027290 Rw2G011670 Rw2G039270 Rw6G018440 Rw6G023120 Rw7G025180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AccIII TCCGGA 1 cut(s) 156
AciI CCGC 2 cut(s) 53, 137
AclWI GGATC 5 cut(s) 165, 371, 465, 509, 537
AcsI RAATTY 1 cut(s) 86
AcuI CTGAAG 1 cut(s) 362
AfiI CCNNNNNNNGG 3 cut(s) 163, 180, 211
AgsI TTSAA 1 cut(s) 290
AjnI CCWGG 3 cut(s) 8, 57, 209
AluBI AGCT 2 cut(s) 349, 485
AluI AGCT 2 cut(s) 349, 485
AlwI GGATC 5 cut(s) 165, 371, 465, 509, 537
Ama87I CYCGRG 1 cut(s) 486
Aor13HI TCCGGA 1 cut(s) 156
ApoI RAATTY 1 cut(s) 86
AspS9I GGNCC 3 cut(s) 55, 202, 275
AsuC2I CCSGG 2 cut(s) 78, 393
AsuHPI GGTGA 3 cut(s) 53, 227, 355
AsuNHI GCTAGC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 486
AvaII GGWCC 3 cut(s) 55, 202, 275
AxyI CCTNAGG 1 cut(s) 68
BbvCI CCTCAGC 1 cut(s) 339
BccI CCATC 2 cut(s) 314, 515
BciT130I CCWGG 3 cut(s) 10, 59, 211
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 2 cut(s) 78, 393
BfaI CTAG 1 cut(s) 14
Bme1390I CCNGG 5 cut(s) 10, 59, 78, 211, 393
Bme18I GGWCC 3 cut(s) 55, 202, 275
BmeT110I CYCGRG 1 cut(s) 486
BmgT120I GGNCC 3 cut(s) 55, 202, 275
BmiI GGNNCC 2 cut(s) 65, 75
BmrFI CCNGG 5 cut(s) 10, 59, 78, 211, 393
BmsI GCATC 1 cut(s) 393
BmtI GCTAGC 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 339
BpuMI CCSGG 2 cut(s) 78, 393
BsaJI CCNNGG 4 cut(s) 58, 209, 210, 278
BsaWI WCCGGW 2 cut(s) 156, 517
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 180, 211
Bse118I RCCGGY 1 cut(s) 99
Bse21I CCTNAGG 1 cut(s) 68
BseAI TCCGGA 1 cut(s) 156
BseBI CCWGG 3 cut(s) 10, 59, 211
BseDI CCNNGG 4 cut(s) 58, 209, 210, 278
BseGI GGATG 1 cut(s) 384
BseLI CCNNNNNNNGG 3 cut(s) 163, 180, 211
BseMII CTCAG 2 cut(s) 46, 330
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 5 cut(s) 77, 100, 157, 392, 518
BslFI GGGAC 1 cut(s) 445
BslI CCNNNNNNNGG 3 cut(s) 163, 180, 211
BsmFI GGGAC 1 cut(s) 445
BsoBI CYCGRG 1 cut(s) 486
Bsp13I TCCGGA 1 cut(s) 156
Bsp143I GATC 6 cut(s) 170, 217, 363, 457, 514, 529
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 2 cut(s) 53, 137
BspCNI CTCAG 2 cut(s) 45, 331
BspEI TCCGGA 1 cut(s) 156
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 65, 75
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 5 cut(s) 165, 371, 465, 509, 537
BspQI GCTCTTC 1 cut(s) 474
BsrFI RCCGGY 1 cut(s) 99
BssAI RCCGGY 1 cut(s) 99
BssECI CCNNGG 4 cut(s) 58, 209, 210, 278
BssMI GATC 6 cut(s) 170, 217, 363, 457, 514, 529
BssT1I CCWWGG 1 cut(s) 278
Bst2UI CCWGG 3 cut(s) 10, 59, 211
Bst4CI ACNGT 3 cut(s) 196, 415, 556
Bst6I CTCTTC 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 15, 97
BstDEI CTNAG 3 cut(s) 32, 68, 339
BstDSI CCRYGG 1 cut(s) 278
BstF5I GGATG 1 cut(s) 384
BstKTI GATC 6 cut(s) 173, 220, 366, 460, 517, 532
BstMBI GATC 6 cut(s) 170, 217, 363, 457, 514, 529
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNI CCWGG 3 cut(s) 10, 59, 211
BstSCI CCNGG 5 cut(s) 8, 57, 76, 209, 391
BstX2I RGATCY 1 cut(s) 363
BstYI RGATCY 1 cut(s) 363
Bsu36I CCTNAGG 1 cut(s) 68
BtgI CCRYGG 1 cut(s) 278
BtsCI GGATG 1 cut(s) 384
BtsIMutI CAGTG 1 cut(s) 552
Cac8I GCNNGC 2 cut(s) 15, 97
CciI TCATGA 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 99
Cfr13I GGNCC 3 cut(s) 55, 202, 275
CseI GACGC 1 cut(s) 512
CviAII CATG 4 cut(s) 175, 242, 279, 476
CviJI RGCY 8 cut(s) 13, 184, 296, 324, 349, 467, 485, 496
CviKI_1 RGCY 8 cut(s) 13, 184, 296, 324, 349, 467, 485, 496
DdeI CTNAG 3 cut(s) 32, 68, 339
DpnI GATC 6 cut(s) 172, 219, 365, 459, 516, 531
DpnII GATC 6 cut(s) 170, 217, 363, 457, 514, 529
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
EciI GGCGGA 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 278
Eco47I GGWCC 3 cut(s) 55, 202, 275
Eco57I CTGAAG 1 cut(s) 362
Eco81I CCTNAGG 1 cut(s) 68
Eco88I CYCGRG 1 cut(s) 486
EcoRII CCWGG 3 cut(s) 8, 57, 209
EcoT14I CCWWGG 1 cut(s) 278
ErhI CCWWGG 1 cut(s) 278
FaeI CATG 4 cut(s) 178, 245, 282, 479
FaiI YATR 6 cut(s) 176, 243, 280, 299, 318, 477
FaqI GGGAC 1 cut(s) 445
FatI CATG 4 cut(s) 174, 241, 278, 475
FbaI TGATCA 1 cut(s) 217
FokI GGATG 1 cut(s) 371
FspBI CTAG 1 cut(s) 14
HapII CCGG 5 cut(s) 77, 100, 157, 392, 518
HgaI GACGC 1 cut(s) 512
Hin1II CATG 4 cut(s) 178, 245, 282, 479
HinfI GANTC 1 cut(s) 149
HpaII CCGG 5 cut(s) 77, 100, 157, 392, 518
HphI GGTGA 3 cut(s) 53, 227, 355
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 2 cut(s) 35, 331
Hpy188III TCNNGA 5 cut(s) 157, 242, 361, 400, 527
Hpy8I GTNNAC 1 cut(s) 237
HpyCH4III ACNGT 3 cut(s) 196, 415, 556
HpyCH4IV ACGT 2 cut(s) 233, 247
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpyF3I CTNAG 3 cut(s) 32, 68, 339
HpySE526I ACGT 2 cut(s) 233, 247
Hsp92II CATG 4 cut(s) 178, 245, 282, 479
Kpn2I TCCGGA 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 6 cut(s) 170, 217, 363, 457, 514, 529
LguI GCTCTTC 1 cut(s) 474
LweI GCATC 1 cut(s) 393
MaeI CTAG 1 cut(s) 14
MaeII ACGT 2 cut(s) 233, 247
MalI GATC 6 cut(s) 172, 219, 365, 459, 516, 531
MboI GATC 6 cut(s) 170, 217, 363, 457, 514, 529
MboII GAAGA 2 cut(s) 56, 461
MflI RGATCY 1 cut(s) 363
MluCI AATT 3 cut(s) 86, 221, 352
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 8 cut(s) 31, 174, 183, 193, 266, 334, 422, 436
MroI TCCGGA 1 cut(s) 156
MslI CAYNNNNRTG 1 cut(s) 246
MspI CCGG 5 cut(s) 77, 100, 157, 392, 518
MspR9I CCNGG 5 cut(s) 10, 59, 78, 211, 393
MvaI CCWGG 3 cut(s) 10, 59, 211
MwoI GCNNNNNNNGC 1 cut(s) 14
NciI CCSGG 2 cut(s) 78, 393
NcoI CCATGG 1 cut(s) 278
NdeII GATC 6 cut(s) 170, 217, 363, 457, 514, 529
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 4 cut(s) 178, 245, 282, 479
NlaIV GGNNCC 2 cut(s) 65, 75
PagI TCATGA 1 cut(s) 241
PasI CCCWGGG 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 474
PfeI GAWTC 1 cut(s) 149
PfoI TCCNGGA 1 cut(s) 76
Psp6I CCWGG 3 cut(s) 8, 57, 209
PspGI CCWGG 3 cut(s) 8, 57, 209
PspN4I GGNNCC 2 cut(s) 65, 75
PspPI GGNCC 3 cut(s) 55, 202, 275
PsuI RGATCY 1 cut(s) 363
RseI CAYNNNNRTG 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 474
Sau3AI GATC 6 cut(s) 170, 217, 363, 457, 514, 529
Sau96I GGNCC 3 cut(s) 55, 202, 275
ScrFI CCNGG 5 cut(s) 10, 59, 78, 211, 393
SfaNI GCATC 1 cut(s) 393
SinI GGWCC 3 cut(s) 55, 202, 275
SmiMI CAYNNNNRTG 1 cut(s) 246
Sse9I AATT 3 cut(s) 86, 221, 352
SsiI CCGC 2 cut(s) 53, 137
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 5 cut(s) 8, 57, 76, 209, 391
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 3 cut(s) 196, 415, 556
TaiI ACGT 2 cut(s) 236, 250
TaqI TCGA 2 cut(s) 169, 532
TasI AATT 3 cut(s) 86, 221, 352
TfiI GAWTC 1 cut(s) 149
TscAI CASTG 1 cut(s) 559
TspDTI ATGAA 1 cut(s) 258
TspRI CASTG 1 cut(s) 559
VpaK11BI GGWCC 3 cut(s) 55, 202, 275
XapI RAATTY 1 cut(s) 86
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.