MD02G1302100.v1.1

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000036
Physical Location & Seq
Reverse (-)
35645835 .. 35650550
4716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1302100.v1.1.491

Sequence Viewer

Length: 3591 bp
ATGGCTCTTGTGAGAACATCTGAAGGAACCTCTTCTGATTCCAAAACTTTTCGGGGTTATCGCCACGACGTGTTCTTGAGCTTCAGAGGCGACACTCGCAAGACTTTTACCGACCACCTCTACACAGCCTTGAACAACGCAGGATTTAACACGTTCCGAGACGACGATGAACTTGAGAGAGGAGAAGATATAAAGCCAGGACTGCAGAAAGCGATCTGGCAGTCACGAACTTCTGTTGTCGTGTTTTCGAAAGATTACGCGTCATCCAAATGGTGCCTTGATGAGCTTGTGTTGATCCTTGAACGCAAGAGGACTACCTCGGACCATGTAGTTTTACCAGTCTTCTACCATGTCGATCCGTCCGACATGAGGAAGCAGACGGGAAGTATTGGAAAAGCAATTGCTAGACACCAGAAAACTCAGTCGCCGGAAAAGGTGAAGGGATGGAGGGAGGCAGTTGCAGAGGTTGCAGATCTAGCAGGCATGGTCTTACAAAATCAAGCAGATGGGTATGAATCAAAGTTTATCGAAAAAATTGTTAAAGTGGTTGGAGACAAGTTAAGTCGTACATCATTGAGTGTTGAACCAAAATTGATTGGAATCGAATCTCAAGTCGAAGAGATCAATTTGTGGTTACAAGATGGATCAACCGATGTCGGTATACTGGTTGTGTATGGCATGTCTGGAATAGGGAAGACAACCATTGCAAAACATGTTTACAATTCAAACTTTAGAAGCTTTAAAGGAAGTAGTTTCATTGAAAATATCAGAGAAACAGCAGATCGACCAAATGGCTTAGTTCAAATACAAAAGCAACTTCTTTTTGATATTTTGAACGGCAGAGAAGTGAAAATACATGGTGTTAGTGAGGGATTAATAAAGATTGAAAGTGCCATAAGCTCTAGAAGAGTGCTTCTTGTTCTTGATGATGTGGACCATATGGACCAATTAGATGCAGTGCTAAAGATGAAAGATCGGTTTTATCCGGGAAGTAAGATACTTATAACAACTAGGCGTGAAAGGTTGCTAAAGGCGCATCAAGTTACGAAGGTGCACAAAGTTGGAACTTTGCATGACAATGAGTCATTAGAGCTTTTCAGTTGGCATGCTTTTCGCCAGGACCATCCCCTTAGAGGTTACATGGAATATTCAGAAAAGGTTGTACACTACTGCGACGGACTTCCACTAGCTCTAAAAGTTTTGGGCTCTTCTTTATCAGGGGAAAGTATAGATGTATGGGAAAGTGCATTGGAGAAGCTACAAGTTTTCCCTAATGGTGAAATCATGAATAAACTAAGAATAAGCTATGACAGTTTACAAGATGACCATGACCGGAAATTATTCCTCCACATTGCTTGTTTTCTAATAGGAAGGAACAAAAGCTACATTGTTAGAAGACTCAATGGATGTGATTTCTATACAATCGTTGGGATTCAAAATCTGCTTGATAGATGCTTGGTGACAATTGATGGATGCAACAACGTGCAAATGCATGACATGATTCGTGACATGGGAAGAGAAATTGTTCGCCACGAATCAGAAGAGCCTGGAAAACGCAGTAGACTATGGTGCTACAAGGATTCTTTCGAAGTATTGAAGGAAAAGAATGGTACAGAAAAAATCCAAGGTCTTGTCCTGAATATGCATATGCACCCTGCAAACAGTTCAATAAACACAAATGAGACAGTCTTGGAAACCAATGCATTTGAAAGGATGCGCAATTTACAACTACTGCATCTTAGTCATGTACAACTGGATGGATGTTATGCAGATTTCCCTACAGGATTAAGATGGTTATGTTGGCTTCAATTTCCTTTGGATTCTATACCTATAGATTTTTCTTTGGAGAAAGTGGTATTTCTTGAAATGCAATATAGCAGCTTGAGACAAGTCTTCAAAGGAACAAAATTTCTTCCATCGTTGAAGATCCTTGATGTGAGCCATTCTCATGGCCTCACTGAAATCATGGACTTCTCACAATGCCCCAGTCTAGAAGAATTGATTCTTGTAGATTGCACAAGCCTGATTGTTGTTCATGAATCCATTGGAAACCTGGAGAGACTCGTGTACTTGAATATGAAGAATTGCAAGAATCTTAGGATGCTTCCGAAGAGCATGTGTATGCTTAAATCACTTGAAACACTCATTTTATCTGGTTGCTCAAATCTTGATGAGTTTCCATTCGAGATGATGCGGAGGATGGAGTCTCTGAAAGTGCTTAAAATCGATGGAATTCCAATACGTGATGTATGGCCAGAAACAAGTTCAAGTATCTTGAGTTATTTTCCATGCTCTTTGGTAGAGTTGAATCTCAGTAGCTGCAATCTTTCTGATGATGCATTTCGTGGGGATTTAAGTAGTCTATCCTCAATACAAAGACTATATTTAAATGAGAATCCAATTTGTACTCTGCCAGTTTTCATCAAAGGTTTGATGAAGCTCAATCATCTCAATTTCTGTGGTTGTACGAAGCTCGAATCGCTTGTGGGGTTACCGAAAGTACACAAAGCGATGGATTTAGCAGTGATGAATTTAAACGGATGCATATCATTAAAAAAAATAACATATCAATCCGGGTTTCAAGAAAATGTTTCTTTGTGGGACAACTGCAATCTAGTTGAGTGGGAGTGCTGGTACAAGATAGAGCCTATTGATAGAGTTGATGTGGAAATGATCAAACTTTTGAGCTTGTTCAACATAGGATCCATGCCAGCCATTAGAATGAACCTCCCTCCATTCGAAAGTTCAAGGCCATATCAGGGTAACTGGAATCCCGTCCAGGGACTGTATCAATTTGGTATATTTAGCACATTTTTTGTTGGGAATGAGGTTCCACGCCAGTTCAGCTATAAAAGTACCAAGTCTTCTATATCTTTTATGGTCCCTTTACTCCCTGCTACTCACAGAATCCGAGGCTTGAACATCTTTGCTACCTATGCAACCAAAGAGAATTCTAATAATCACCATTATAGTATGAATCGTCCAATAATGACTAAAGTGAGTAACAAGAGCAAGGGTCTGAAGTGGATCTATGCCCCACTGTTCTTTGGAATTCCAGGAGATAGTGAAGATATGATATGGTTAAGCCATTGGAGGATGGAGAGTGAAACAATATTACAATGTGGAGATCAAGTGGTTGTTTCGGTAATGGCCGGATTTTCTGAGCTGTATCGGGTAAAGGAGTTTGGTGTCGAGCTTGTGCAAGAGCACCAGAATAATATGATGATAAGTACTCAACATAATACCAAATCAGATCCTCATTATCCATTTGTCATCGGTGGAGATTTGTCGATGTGGGAGCATAAACCAGGAATATACATCCTTGGTTTCTCTAAAGAATATGCTGAAAAAAATTTTGATTCCCGTAAACAAGGAATGCTTAATCGCCTCATCATGGACACTGATGAAGAAGACACAGGCAAAGAAGAAGGGCAAGAGGGTGAACCTGATTACACAATTGTAAGGATGAGGGCTGCCAGCAACAACTGCACCCTCGGAGGCTGGAAGCTGCTCCTCACAGCTGCCGGCTTATTTTTCACGCTTGCTCTTGTAGTTTGGTCCTCCATCTGTCAGAAAAAGAAGCGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1197

Amino Acids

136.52

Weight (kDa)

6.68

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 193 1.8e-50 TIR domain
TIR_2 PF13676 24 - 120 2.6e-15 TIR domain
NB-ARC PF00931 202 - 373 3.6e-21 NB-ARC domain
WHD_ROQ1 PF23282 443 - 513 6.4e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 637 - 725 7.9e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1004
Acc16I TGCGCA 1 cut(s) 1718
AccB1I GGYRCC 1 cut(s) 273
AccI GTMKAC 2 cut(s) 661, 1561
AccII CGCG 1 cut(s) 260
AciI CCGC 1 cut(s) 2194
AclWI GGATC 8 cut(s) 289, 350, 652, 1921, 2697, 2710, 3035, 3248
AcoI YGGCCR 2 cut(s) 2252, 3150
AcsI RAATTY 6 cut(s) 1907, 2232, 2530, 2950, 3051, 3352
AcuI CTGAAG 3 cut(s) 42, 67, 3041
AdeI CACNNNGTG 1 cut(s) 70
AfiI CCNNNNNNNGG 5 cut(s) 369, 1133, 2759, 2780, 3394
AflIII ACRYGT 4 cut(s) 69, 150, 258, 712
AhdI GACNNNNNGTC 1 cut(s) 1081
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 7 cut(s) 196, 1116, 1546, 2052, 2778, 3055, 3307
Alw21I GWGCWC 2 cut(s) 1056, 3210
Alw26I GTCTC 6 cut(s) 153, 546, 1676, 1879, 2053, 2211
Alw44I GTGCAC 1 cut(s) 1052
AlwI GGATC 8 cut(s) 289, 350, 652, 1921, 2697, 2710, 3035, 3248
AlwNI CAGNNNCTG 2 cut(s) 2319, 2785
AoxI GGCC 4 cut(s) 1951, 2252, 2750, 3150
ApaLI GTGCAC 1 cut(s) 1052
ApeKI GCWGC 5 cut(s) 1878, 2319, 3473, 3508, 3521
ApoI RAATTY 6 cut(s) 1907, 2232, 2530, 2950, 3051, 3352
ArsI GACNNNNNNTTYG 2 cut(s) 597, 629
AseI ATTAAT 1 cut(s) 875
Asp700I GAANNNNTTC 4 cut(s) 31, 1340, 1524, 2001
AspLEI GCGC 2 cut(s) 1036, 1719
AspS9I GGNCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
AsuC2I CCSGG 2 cut(s) 987, 2575
AsuHPI GGTGA 5 cut(s) 448, 1289, 1471, 2954, 3452
AsuII TTCGAA 3 cut(s) 248, 1587, 2739
AvaII GGWCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
BaeGI GKGCMC 1 cut(s) 1056
BalI TGGCCA 1 cut(s) 2254
BamHI GGATCC 1 cut(s) 2702
BanI GGYRCC 1 cut(s) 273
BanII GRGCYC 1 cut(s) 1208
BarI GAAGNNNNNNTAC 4 cut(s) 837, 869, 2848, 2880
BauI CACGAG 1 cut(s) 2063
BbsI GAAGAC 6 cut(s) 334, 701, 1402, 1885, 2856, 3417
Bbv12I GWGCWC 2 cut(s) 1056, 3210
BbvI GCAGC 5 cut(s) 1890, 2306, 3460, 3495, 3508
BceAI ACGGC 1 cut(s) 853
BcgI CGANNNNNNTGC 2 cut(s) 1094, 1128
BciT130I CCWGG 7 cut(s) 198, 1118, 1548, 2054, 2780, 3057, 3309
BclI TGATCA 1 cut(s) 2673
BcnI CCSGG 2 cut(s) 987, 2575
BcoDI GTCTC 6 cut(s) 153, 546, 1676, 1879, 2053, 2211
BfaI CTAG 7 cut(s) 405, 476, 903, 1011, 1187, 1991, 2615
BfmI CTRYAG 3 cut(s) 203, 1779, 1830
BglII AGATCT 1 cut(s) 472
BisI GCNGC 5 cut(s) 1879, 2320, 3474, 3509, 3522
BlsI GCNGC 5 cut(s) 1880, 2321, 3475, 3510, 3523
BmcAI AGTACT 1 cut(s) 3232
Bme1390I CCNGG 9 cut(s) 198, 987, 1118, 1548, 2054, 2575, 2780, 3057, 3309
Bme18I GGWCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
BmeRI GACNNNNNGTC 1 cut(s) 1081
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
BmiI GGNNCC 5 cut(s) 28, 275, 2704, 2832, 2883
BmrFI CCNGG 9 cut(s) 198, 987, 1118, 1548, 2054, 2575, 2780, 3057, 3309
BmrI ACTGGG 1 cut(s) 1980
BmuI ACTGGG 1 cut(s) 1980
BpiI GAAGAC 6 cut(s) 334, 701, 1402, 1885, 2856, 3417
BplI GAGNNNNNCTC 2 cut(s) 1930, 1962
BpmI CTGGAG 1 cut(s) 2075
Bpu14I TTCGAA 3 cut(s) 248, 1587, 2739
BpuEI CTTGAG 5 cut(s) 97, 194, 594, 1903, 2296
BpuMI CCSGG 2 cut(s) 987, 2575
Bsa29I ATCGAT 1 cut(s) 2226
BsaAI YACGTR 1 cut(s) 2243
BsaBI GATNNNNATC 2 cut(s) 599, 2545
BsaJI CCNNGG 6 cut(s) 318, 1624, 2779, 2911, 3322, 3493
BsaWI WCCGGW 1 cut(s) 1332
BsaXI ACNNNNNCTCC 4 cut(s) 2618, 2648, 3117, 3147
Bsc4I CCNNNNNNNGG 5 cut(s) 369, 1133, 2759, 2780, 3394
Bse118I RCCGGY 1 cut(s) 3524
Bse1I ACTGG 7 cut(s) 338, 669, 1758, 1986, 2414, 2771, 2839
Bse3DI GCAATG 2 cut(s) 702, 1350
Bse8I GATNNNNATC 2 cut(s) 599, 2545
BseBI CCWGG 7 cut(s) 198, 1118, 1548, 2054, 2780, 3057, 3309
BseCI ATCGAT 1 cut(s) 2226
BseDI CCNNGG 6 cut(s) 318, 1624, 2779, 2911, 3322, 3493
BseJI GATNNNNATC 2 cut(s) 599, 2545
BseLI CCNNNNNNNGG 5 cut(s) 369, 1133, 2759, 2780, 3394
BseMI GCAATG 2 cut(s) 702, 1350
BseMII CTCAG 3 cut(s) 434, 2326, 3153
BseNI ACTGG 7 cut(s) 338, 669, 1758, 1986, 2414, 2771, 2839
BseRI GAGGAG 2 cut(s) 195, 3503
BseSI GKGCMC 1 cut(s) 1056
BseXI GCAGC 5 cut(s) 1890, 2306, 3460, 3495, 3508
BsgI GTGCAG 1 cut(s) 3472
Bsh1236I CGCG 1 cut(s) 260
BshFI GGCC 4 cut(s) 1953, 2254, 2752, 3152
BshNI GGYRCC 1 cut(s) 273
BshVI ATCGAT 1 cut(s) 2226
BsiHKAI GWGCWC 2 cut(s) 1056, 3210
BsiSI CCGG 6 cut(s) 428, 986, 1333, 2574, 3153, 3525
BslFI GGGAC 3 cut(s) 2615, 2796, 2867
BslI CCNNNNNNNGG 5 cut(s) 369, 1133, 2759, 2780, 3394
BsmAI GTCTC 6 cut(s) 153, 546, 1676, 1879, 2053, 2211
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 3 cut(s) 2615, 2796, 2867
BsmI GAATGC 1 cut(s) 3381
BsnI GGCC 4 cut(s) 1953, 2254, 2752, 3152
Bsp119I TTCGAA 3 cut(s) 248, 1587, 2739
Bsp1286I GDGCHC 3 cut(s) 1056, 1208, 3210
Bsp1407I TGTACA 2 cut(s) 1162, 1747
BspACI CCGC 1 cut(s) 2194
BspANI GGCC 4 cut(s) 1953, 2254, 2752, 3152
BspCNI CTCAG 3 cut(s) 433, 2325, 3154
BspDI ATCGAT 1 cut(s) 2226
BspFNI CGCG 1 cut(s) 260
BspHI TCATGA 2 cut(s) 1284, 2035
BspLI GGNNCC 5 cut(s) 28, 275, 2704, 2832, 2883
BspMAI CTGCAG 1 cut(s) 207
BspPI GGATC 8 cut(s) 289, 350, 652, 1921, 2697, 2710, 3035, 3248
BspQI GCTCTTC 3 cut(s) 1213, 1536, 2105
BspT104I TTCGAA 3 cut(s) 248, 1587, 2739
BspT107I GGYRCC 1 cut(s) 273
BsrDI GCAATG 2 cut(s) 702, 1350
BsrFI RCCGGY 1 cut(s) 3524
BsrGI TGTACA 2 cut(s) 1162, 1747
BsrI ACTGG 7 cut(s) 338, 669, 1758, 1986, 2414, 2771, 2839
BssAI RCCGGY 1 cut(s) 3524
BssECI CCNNGG 6 cut(s) 318, 1624, 2779, 2911, 3322, 3493
BssNAI GTATAC 1 cut(s) 662
BssSI CACGAG 1 cut(s) 2063
BssT1I CCWWGG 2 cut(s) 1624, 3322
Bst1107I GTATAC 1 cut(s) 662
Bst2BI CACGAG 1 cut(s) 2063
Bst2UI CCWGG 7 cut(s) 198, 1118, 1548, 2054, 2780, 3057, 3309
Bst4CI ACNGT 5 cut(s) 1313, 1664, 1687, 2787, 3042
Bst6I CTCTTC 7 cut(s) 37, 612, 901, 1213, 1510, 1536, 2105
BstAPI GCANNNNNTGC 2 cut(s) 467, 3486
BstAUI TGTACA 2 cut(s) 1162, 1747
BstBAI YACGTR 1 cut(s) 2243
BstBI TTCGAA 3 cut(s) 248, 1587, 2739
BstC8I GCNNGC 6 cut(s) 481, 1107, 2712, 3478, 3526, 3543
BstDEI CTNAG 8 cut(s) 420, 796, 1130, 1295, 1739, 2096, 2312, 3162
BstEII GGTNACC 1 cut(s) 2490
BstFNI CGCG 1 cut(s) 260
BstHHI GCGC 2 cut(s) 1036, 1719
BstMAI GTCTC 6 cut(s) 153, 546, 1676, 1879, 2053, 2211
BstNI CCWGG 7 cut(s) 198, 1118, 1548, 2054, 2780, 3057, 3309
BstNSI RCATGY 4 cut(s) 682, 716, 1109, 2119
BstPI GGTNACC 1 cut(s) 2490
BstSCI CCNGG 9 cut(s) 196, 985, 1116, 1546, 2052, 2573, 2778, 3055, 3307
BstSFI CTRYAG 3 cut(s) 203, 1779, 1830
BstSLI GKGCMC 1 cut(s) 1056
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 5 cut(s) 1890, 2306, 3460, 3495, 3508
BstV2I GAAGAC 6 cut(s) 334, 701, 1402, 1885, 2856, 3417
BstX2I RGATCY 5 cut(s) 472, 1926, 2702, 3027, 3253
BstXI CCANNNNNNTGG 1 cut(s) 1949
BstYI RGATCY 5 cut(s) 472, 1926, 2702, 3027, 3253
BstZ17I GTATAC 1 cut(s) 662
Bsu15I ATCGAT 1 cut(s) 2226
BsuRI GGCC 4 cut(s) 1953, 2254, 2752, 3152
BsuTUI ATCGAT 1 cut(s) 2226
BtgZI GCGATG 1 cut(s) 2525
BtrI CACGTC 1 cut(s) 70
BtsI GCAGTG 2 cut(s) 963, 2529
BtsIMutI CAGTG 5 cut(s) 963, 1956, 2529, 3038, 3399
Cac8I GCNNGC 6 cut(s) 481, 1107, 2712, 3478, 3526, 3543
CaiI CAGNNNCTG 2 cut(s) 2319, 2785
CciI TCATGA 2 cut(s) 1284, 2035
CfoI GCGC 2 cut(s) 1036, 1719
Cfr10I RCCGGY 1 cut(s) 3524
Cfr13I GGNCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
ClaI ATCGAT 1 cut(s) 2226
CseI GACGC 1 cut(s) 249
CspCI CAANNNNNGTGG 4 cut(s) 1337, 1372, 2440, 2475
DdeI CTNAG 8 cut(s) 420, 796, 1130, 1295, 1739, 2096, 2312, 3162
DraI TTTAAA 3 cut(s) 742, 2388, 2535
DraIII CACNNNGTG 1 cut(s) 70
DriI GACNNNNNGTC 1 cut(s) 1081
EaeI YGGCCR 2 cut(s) 2252, 3150
Eam1104I CTCTTC 7 cut(s) 37, 612, 901, 1213, 1510, 1536, 2105
Eam1105I GACNNNNNGTC 1 cut(s) 1081
EarI CTCTTC 7 cut(s) 37, 612, 901, 1213, 1510, 1536, 2105
Eco130I CCWWGG 2 cut(s) 1624, 3322
Eco24I GRGCYC 1 cut(s) 1208
Eco47I GGWCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
Eco57I CTGAAG 3 cut(s) 42, 67, 3041
Eco91I GGTNACC 1 cut(s) 2490
EcoO65I GGTNACC 1 cut(s) 2490
EcoRI GAATTC 3 cut(s) 2232, 2950, 3051
EcoRII CCWGG 7 cut(s) 196, 1116, 1546, 2052, 2778, 3055, 3307
EcoT14I CCWWGG 2 cut(s) 1624, 3322
EcoT22I ATGCAT 5 cut(s) 1494, 1647, 1705, 2341, 2546
EcoT38I GRGCYC 1 cut(s) 1208
ErhI CCWWGG 2 cut(s) 1624, 3322
Esp3I CGTCTC 1 cut(s) 153
FalI AAGNNNNNCTT 2 cut(s) 3363, 3395
FaqI GGGAC 3 cut(s) 2615, 2796, 2867
FauNDI CATATG 2 cut(s) 939, 1647
FbaI TGATCA 1 cut(s) 2673
FblI GTMKAC 2 cut(s) 661, 1561
Fnu4HI GCNGC 5 cut(s) 1879, 2320, 3474, 3509, 3522
FriOI GRGCYC 1 cut(s) 1208
Fsp4HI GCNGC 5 cut(s) 1879, 2320, 3474, 3509, 3522
FspBI CTAG 7 cut(s) 405, 476, 903, 1011, 1187, 1991, 2615
FspI TGCGCA 1 cut(s) 1718
GlaI GCGC 2 cut(s) 1035, 1718
GluI GCNGC 5 cut(s) 1879, 2320, 3474, 3509, 3522
GsuI CTGGAG 1 cut(s) 2075
HaeIII GGCC 4 cut(s) 1953, 2254, 2752, 3152
HapII CCGG 6 cut(s) 428, 986, 1333, 2574, 3153, 3525
HgaI GACGC 1 cut(s) 249
HhaI GCGC 2 cut(s) 1036, 1719
Hin6I GCGC 2 cut(s) 1034, 1717
HinP1I GCGC 2 cut(s) 1034, 1717
HindIII AAGCTT 1 cut(s) 736
HpaII CCGG 6 cut(s) 428, 986, 1333, 2574, 3153, 3525
HphI GGTGA 5 cut(s) 448, 1289, 1471, 2954, 3452
Hpy99I CGWCG 3 cut(s) 71, 167, 1178
HpyAV CCTTC 6 cut(s) 17, 433, 1042, 1365, 1591, 3422
HpyCH4III ACNGT 5 cut(s) 1313, 1664, 1687, 2787, 3042
HpyCH4IV ACGT 4 cut(s) 69, 152, 1482, 2242
HpyF3I CTNAG 8 cut(s) 420, 796, 1130, 1295, 1739, 2096, 2312, 3162
HpySE526I ACGT 4 cut(s) 69, 152, 1482, 2242
HspAI GCGC 2 cut(s) 1034, 1717
KroI GCCGGC 1 cut(s) 3524
KroNI GCCGGC 1 cut(s) 3526
Ksp22I TGATCA 1 cut(s) 2673
LguI GCTCTTC 3 cut(s) 1213, 1536, 2105
LmnI GCTCC 2 cut(s) 3298, 3516
Lsp1109I GCAGC 5 cut(s) 1890, 2306, 3460, 3495, 3508
MaeI CTAG 7 cut(s) 405, 476, 903, 1011, 1187, 1991, 2615
MaeII ACGT 4 cut(s) 69, 152, 1482, 2242
MaeIII GTNAC 9 cut(s) 222, 633, 1042, 1136, 1459, 1505, 2490, 2762, 3002
MfeI CAATTG 3 cut(s) 399, 1464, 3456
MflI RGATCY 5 cut(s) 472, 1926, 2702, 3027, 3253
MhlI GDGCHC 3 cut(s) 1056, 1208, 3210
MlsI TGGCCA 1 cut(s) 2254
MluI ACGCGT 1 cut(s) 258
MluNI TGGCCA 1 cut(s) 2254
MlyI GAGTC 4 cut(s) 1091, 1392, 2055, 2213
MmeI TCCRAC 3 cut(s) 387, 529, 1042
Mox20I TGGCCA 1 cut(s) 2254
Mph1103I ATGCAT 5 cut(s) 1494, 1647, 1705, 2341, 2546
MroNI GCCGGC 1 cut(s) 3524
MroXI GAANNNNTTC 4 cut(s) 31, 1340, 1524, 2001
MscI TGGCCA 1 cut(s) 2254
MslI CAYNNNNRTG 5 cut(s) 268, 1077, 1947, 2120, 2720
Msp20I TGGCCA 1 cut(s) 2254
MspA1I CMGCKG 1 cut(s) 3521
MspI CCGG 6 cut(s) 428, 986, 1333, 2574, 3153, 3525
MspR9I CCNGG 9 cut(s) 198, 987, 1118, 1548, 2054, 2575, 2780, 3057, 3309
MunI CAATTG 3 cut(s) 399, 1464, 3456
Mva1269I GAATGC 1 cut(s) 3381
MvaI CCWGG 7 cut(s) 198, 1118, 1548, 2054, 2780, 3057, 3309
MvnI CGCG 1 cut(s) 260
NaeI GCCGGC 1 cut(s) 3526
NciI CCSGG 2 cut(s) 987, 2575
NdeI CATATG 2 cut(s) 939, 1647
NgoMIV GCCGGC 1 cut(s) 3524
NlaIV GGNNCC 5 cut(s) 28, 275, 2704, 2832, 2883
NmuCI GTSAC 3 cut(s) 222, 1459, 1505
NsbI TGCGCA 1 cut(s) 1718
NsiI ATGCAT 5 cut(s) 1494, 1647, 1705, 2341, 2546
NspI RCATGY 4 cut(s) 682, 716, 1109, 2119
NspV TTCGAA 3 cut(s) 248, 1587, 2739
PaeI GCATGC 1 cut(s) 1109
PagI TCATGA 2 cut(s) 1284, 2035
PciI ACATGT 1 cut(s) 712
PciSI GCTCTTC 3 cut(s) 1213, 1536, 2105
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 1 cut(s) 3381
PdiI GCCGGC 1 cut(s) 3526
PdmI GAANNNNTTC 4 cut(s) 31, 1340, 1524, 2001
PfoI TCCNGGA 2 cut(s) 985, 3055
PkrI GCNGC 5 cut(s) 1880, 2321, 3475, 3510, 3523
PleI GAGTC 4 cut(s) 1090, 1392, 2055, 2212
PpsI GAGTC 4 cut(s) 1090, 1392, 2055, 2212
Ppu21I YACGTR 1 cut(s) 2243
PscI ACATGT 1 cut(s) 712
PshBI ATTAAT 1 cut(s) 875
PsiI TTATAA 1 cut(s) 1004
Psp6I CCWGG 7 cut(s) 196, 1116, 1546, 2052, 2778, 3055, 3307
PspEI GGTNACC 1 cut(s) 2490
PspGI CCWGG 7 cut(s) 196, 1116, 1546, 2052, 2778, 3055, 3307
PspN4I GGNNCC 5 cut(s) 28, 275, 2704, 2832, 2883
PspPI GGNCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
PsrI GAACNNNNNNTAC 2 cut(s) 1367, 1399
PstI CTGCAG 1 cut(s) 207
PstNI CAGNNNCTG 2 cut(s) 2319, 2785
PsuI RGATCY 5 cut(s) 472, 1926, 2702, 3027, 3253
PvuII CAGCTG 1 cut(s) 3521
RseI CAYNNNNRTG 5 cut(s) 268, 1077, 1947, 2120, 2720
SapI GCTCTTC 3 cut(s) 1213, 1536, 2105
SatI GCNGC 5 cut(s) 1879, 2320, 3474, 3509, 3522
Sau96I GGNCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
ScaI AGTACT 1 cut(s) 3232
SchI GAGTC 4 cut(s) 1091, 1392, 2055, 2213
ScrFI CCNGG 9 cut(s) 198, 987, 1118, 1548, 2054, 2575, 2780, 3057, 3309
SduI GDGCHC 3 cut(s) 1056, 1208, 3210
SfcI CTRYAG 3 cut(s) 203, 1779, 1830
SfuI TTCGAA 3 cut(s) 248, 1587, 2739
SinI GGWCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
SmiI ATTTAAAT 1 cut(s) 2388
SmiMI CAYNNNNRTG 5 cut(s) 268, 1077, 1947, 2120, 2720
SmlI CTYRAG 5 cut(s) 76, 173, 609, 1882, 2275
SmoI CTYRAG 5 cut(s) 76, 173, 609, 1882, 2275
SphI GCATGC 1 cut(s) 1109
SsiI CCGC 1 cut(s) 2194
SspI AATATT 2 cut(s) 1148, 3114
SspMI CTAG 7 cut(s) 405, 476, 903, 1011, 1187, 1991, 2615
StyD4I CCNGG 9 cut(s) 196, 985, 1116, 1546, 2052, 2573, 2778, 3055, 3307
StyI CCWWGG 2 cut(s) 1624, 3322
SwaI ATTTAAAT 1 cut(s) 2388
TaaI ACNGT 5 cut(s) 1313, 1664, 1687, 2787, 3042
TaiI ACGT 4 cut(s) 72, 155, 1485, 2245
TatI WGTACW 6 cut(s) 1162, 1747, 2067, 2405, 2500, 3230
TscAI CASTG 5 cut(s) 963, 1963, 2529, 3045, 3406
TseFI GTSAC 3 cut(s) 222, 1459, 1505
TseI GCWGC 5 cut(s) 1878, 2319, 3473, 3508, 3521
Tsp45I GTSAC 3 cut(s) 222, 1459, 1505
TspGWI ACGGA 3 cut(s) 348, 1191, 2553
TspRI CASTG 5 cut(s) 963, 1963, 2529, 3045, 3406
VneI GTGCAC 1 cut(s) 1052
VpaK11BI GGWCC 6 cut(s) 322, 934, 943, 1120, 2881, 3558
VspI ATTAAT 1 cut(s) 875
XapI RAATTY 6 cut(s) 1907, 2232, 2530, 2950, 3051, 3352
XbaI TCTAGA 2 cut(s) 902, 1990
XceI RCATGY 4 cut(s) 682, 716, 1109, 2119
XcmI CCANNNNNNNNNTGG 1 cut(s) 2050
XmiI GTMKAC 2 cut(s) 661, 1561
XmnI GAANNNNTTC 4 cut(s) 31, 1340, 1524, 2001
XspI CTAG 7 cut(s) 405, 476, 903, 1011, 1187, 1991, 2615
ZrmI AGTACT 1 cut(s) 3232
Zsp2I ATGCAT 5 cut(s) 1494, 1647, 1705, 2341, 2546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.