MD05G1140100.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000036
Physical Location & Seq
Reverse (-)
27077454 .. 27080669
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1140100.v1.1.491

Sequence Viewer

Length: 2496 bp
ATGGCAGGAAAGAATATAGCTGTATGGGAAAGTGCAATAAGCAAACTGGAAGTTATTCCGGACAGTCAAATCTTGAAAAAACTCAAAATAAGCTACGATTCCTTGCAAGATAGCCATGACCAAGATTTATTCCTTCATATTGCCTGCTTCTTTATCGGAATGGAGAAAGATATCATCGTTAGAATACTAGATGCCTGTGATTTTTTCACAGTTGTTGGAATTCAAAATCTCATTGATAGAAATTTGGTAAGACTCGATGGAGATAATATGGTGCAGATGCATCACATGATCCGTGACATGGCAAGAGGAATTGTTCGTCTGGAATCAAAGGAGCCTGGGGAACGTAGTAGATTATGGCATCATAAGGATTCTTTTGCAGTATTGGCAGAAAAGAATGGAACGCAAACAATTGAAGGTCTTGCTCTCAACGTGCAAAACCACCCAGAATACTCTTCTTCAAGAAATTCAAATCAGGTAGTTTTTGAAACGGATGCGTTTTCAATGATGCACAAATTAAGACTTCTGCAGCTTACTTATGTAGAACTCAATGGATCCTATGAAGAATTTCCGACAAGCTTAAGATGGTTGTGTTGGCATAAATTTTTGAAGGGTTCTATACCCTCTGATTTTTCTTTGAAGAATTTGGTTGTTCTGGAAATGTGTTACAGCAGCATGAGGCAAGTCTGGAAGGGAACTAAGTTTCTCCCATCACTAAAGATTCTAAATCTTAGCCATTCCAATGACCTCACCGAAACCCCGGACTTTTCTCTGGTCCCCAATCTAGAATGGCTGATTCTTAAAGATTGTTCAAGCTTGGTTGATGTCCATGAATCCATTGGAAACTTAGAGGGACTTGTGCATTTGAATATGGAATATTGCAAAAGTATTAAGAAGCTTCCAGAGAATATTTCTAAGCTAACCTCCCTTGAAACACTTGTTATATCTGGTTGCTCAAGCCTCAGCGTTTTCCCCATAGACACGAGGAAGATGGAATCTCTGAAAGTGTTTCAAGCTGACGGTGTTCCAATACATCAGTTACTCACTGCTACCGAGGAGGTCACATTATGGCCAAGACGAAATGTAGAAATTACTTGGGCTTCTTATCTACCATGCAATTTAGTAGACTTGAGTCTTAGAAAGTGCAATCTTGGTGATGATGATTTTCCCAGAGAATTTGGCAACTTATCCACGTTGTGTAGCTTAGATCTGGGGGGTAATCCAATTCATAGCCTACCAGATTGCATTAGAGGTGTTACGGGGCTTCGTAGACTCTCTTTTGAGGATTGTAAAAGGCTCAAATCGCTTGTAAGGCTACCAACTGTAGGGCACTTGATAATCGCTGATTGTGAAAAGTTGGAAACGGTAACATTTCAATCATTAAGTGAGGTGTACTCTAGAATGCGCTCCATCGAATGCCGTGGCAACCATAAGCTAGTTGAGTTTGAGTCAGTGTACAAGATGGAGCCTATTGAAAGAGTTGATATAGAAATGATCACCATTTTGGGTTTGAGCAACTTGAAATATTCAACAAAATCCATTATGAAGTACAAGATACCCGCACGGTTCCACAGATGGATAGAAAAGCGTCTTCCCATCCAGGGACTGCATGAATTTGGAATATTCAGCACATTTCTTCCTGGGAATTATGAGGTTCCTCAATATGACTCATTTAGCCATAAAAGTAGAGGGCCCTCAATATCTTTTACTGTGCCTGTACTTCCCTATTGCAGGATCCGAGGCTTGAACATCTTCTCTGTCTATGAAAAATCCAAAAGCGATGAATCTCCTAACATCAGAGTAAAAAATATGAATGGTTTATATTATACAATAATGATAGATGTTAGAAATAAGAGTAAAGGTCTGCAGTGGATCTACGGGCCAGCATTATTCGGAATTCCAAGTGATGATCAAGATGTGATATGGTTAAGCCATTGGAAATTCGGGAATAAATTGGAAGGTGGCGATGAGCTGACTGTTTCAATACTTACATCATCATTTATGATATATGATGAGTTTCAGGTAAAGGAGTTCGGTGTCCAGGTTGTGTACTACGAGCAAGAAGAGAAGGTGACAGTCACCGCCCAAGAAAATTTCAACACAGATAATCCTTTTTATCCACGTGTCATCGCCGGAGATTTGTCACATTATCTGCTGCCAGGATCATCAGGAACATACCTTCTCTGCCATAATCCAGTATTAGAAAGAAGGGATGTGCTAAGTTATGTTTGGTTCAAGCACAATAGTAATGAAATAACAGGCCATGGATCTTTGGGACTTAAATATCAGCTGGTAGAGCAAGAAGAAGATCGAGAAAGTGAATGCACGCTTGTAGAACATGAGGAGAGCAGCAGTAGCAATTGCAATAGTATCGATAGCAGTGGTGCATGCAGAGGTTGGAAAGTGGTACCGAGTATAATGACCATGGCCACTATCTTCTTGAGATGCTTTCAGTTGCACAAATGTTTGTCGCTGGAAGATGGTAACAATGGCGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

832

Amino Acids

94.86

Weight (kDa)

5.94

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 39 - 108 2.6e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 253 - 339 7.9e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2404
AccB1I GGYRCC 1 cut(s) 2404
AccI GTMKAC 2 cut(s) 1122, 1267
AccII CGCG 1 cut(s) 2492
AccIII TCCGGA 1 cut(s) 58
AciI CCGC 2 cut(s) 1557, 2079
AclWI GGATC 8 cut(s) 283, 546, 559, 1726, 1739, 1877, 2167, 2272
AcoI YGGCCR 2 cut(s) 1067, 2424
AcvI CACGTG 1 cut(s) 2120
AdeI CACNNNGTG 1 cut(s) 1194
AfaI GTAC 6 cut(s) 1391, 1454, 1547, 1716, 2048, 2406
AfiI CCNNNNNNNGG 4 cut(s) 298, 1322, 1598, 1728
AflII CTTAAG 1 cut(s) 577
AflIII ACRYGT 1 cut(s) 2119
AjnI CCWGG 5 cut(s) 334, 1596, 1636, 2037, 2155
AlwI GGATC 8 cut(s) 283, 546, 559, 1726, 1739, 1877, 2167, 2272
AlwNI CAGNNNCTG 1 cut(s) 1603
Aor13HI TCCGGA 1 cut(s) 58
AoxI GGCC 5 cut(s) 1067, 1688, 1877, 2257, 2424
ApaI GGGCCC 1 cut(s) 1692
ApeKI GCWGC 4 cut(s) 526, 669, 2152, 2346
Asp700I GAANNNNTTC 3 cut(s) 54, 564, 1748
Asp718I GGTACC 1 cut(s) 2404
AspLEI GCGC 2 cut(s) 1404, 2492
AspS9I GGNCC 4 cut(s) 772, 1688, 1689, 1877
AsuC2I CCSGG 1 cut(s) 758
AsuHPI GGTGA 5 cut(s) 739, 1163, 1486, 2068, 2080
AvaII GGWCC 1 cut(s) 772
BaeGI GKGCMC 2 cut(s) 1329, 1692
BalI TGGCCA 2 cut(s) 1069, 2426
BamHI GGATCC 2 cut(s) 551, 1731
BanI GGYRCC 1 cut(s) 2404
BanII GRGCYC 1 cut(s) 1692
BauI CACGAG 1 cut(s) 979
BbrPI CACGTG 1 cut(s) 2120
BbsI GAAGAC 1 cut(s) 1580
BbvCI CCTCAGC 1 cut(s) 959
BbvI GCAGC 4 cut(s) 538, 681, 2139, 2358
BccI CCATC 9 cut(s) 251, 576, 715, 982, 1415, 1453, 1566, 1601, 2471
BceAI ACGGC 1 cut(s) 1401
BcgI CGANNNNNNTGC 2 cut(s) 2350, 2384
BciT130I CCWGG 5 cut(s) 336, 1598, 1638, 2039, 2157
BclI TGATCA 2 cut(s) 1491, 1906
BcnI CCSGG 1 cut(s) 758
BfaI CTAG 4 cut(s) 188, 782, 1395, 1433
BfmI CTRYAG 3 cut(s) 524, 1320, 1862
BfrI CTTAAG 1 cut(s) 577
BglII AGATCT 1 cut(s) 1204
BisI GCNGC 4 cut(s) 527, 670, 2153, 2347
BlsI GCNGC 4 cut(s) 528, 671, 2154, 2348
Bme1390I CCNGG 6 cut(s) 336, 758, 1598, 1638, 2039, 2157
Bme18I GGWCC 1 cut(s) 772
BmgT120I GGNCC 4 cut(s) 772, 1688, 1689, 1877
BmiI GGNNCC 9 cut(s) 333, 553, 774, 1464, 1565, 1653, 1690, 1733, 2406
BmrFI CCNGG 6 cut(s) 336, 758, 1598, 1638, 2039, 2157
BmsI GCATC 7 cut(s) 181, 267, 289, 367, 481, 495, 2432
BoxI GACNNNNGTC 1 cut(s) 1128
BpiI GAAGAC 1 cut(s) 1580
BplI GAGNNNNNCTC 2 cut(s) 1376, 1408
Bpu10I CCTNAGC 1 cut(s) 959
BpuEI CTTGAG 3 cut(s) 937, 1147, 2458
BpuMI CCSGG 1 cut(s) 758
Bsa29I ATCGAT 1 cut(s) 2370
BsaAI YACGTR 1 cut(s) 2120
BsaJI CCNNGG 9 cut(s) 335, 756, 1050, 1417, 1597, 1637, 1735, 2260, 2421
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 4 cut(s) 298, 1322, 1598, 1728
Bse1I ACTGG 2 cut(s) 51, 2192
BseAI TCCGGA 1 cut(s) 58
BseBI CCWGG 5 cut(s) 336, 1598, 1638, 2039, 2157
BseCI ATCGAT 1 cut(s) 2370
BseDI CCNNGG 9 cut(s) 335, 756, 1050, 1417, 1597, 1637, 1735, 2260, 2421
BseGI GGATG 3 cut(s) 496, 1593, 2215
BseLI CCNNNNNNNGG 4 cut(s) 298, 1322, 1598, 1728
BseMII CTCAG 1 cut(s) 973
BseNI ACTGG 2 cut(s) 51, 2192
BseRI GAGGAG 2 cut(s) 1067, 2354
BseSI GKGCMC 2 cut(s) 1329, 1692
BseXI GCAGC 4 cut(s) 538, 681, 2139, 2358
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 2492
BshFI GGCC 5 cut(s) 1069, 1690, 1879, 2259, 2426
BshNI GGYRCC 1 cut(s) 2404
BshVI ATCGAT 1 cut(s) 2370
BsiSI CCGG 3 cut(s) 59, 758, 2130
BslFI GGGAC 4 cut(s) 758, 864, 1614, 2286
BslI CCNNNNNNNGG 4 cut(s) 298, 1322, 1598, 1728
BsmFI GGGAC 4 cut(s) 758, 864, 1614, 2286
BsmI GAATGC 3 cut(s) 1404, 1418, 2324
BsnI GGCC 5 cut(s) 1069, 1690, 1879, 2259, 2426
Bsp120I GGGCCC 1 cut(s) 1688
Bsp1286I GDGCHC 2 cut(s) 1329, 1692
Bsp13I TCCGGA 1 cut(s) 58
Bsp1407I TGTACA 1 cut(s) 1452
Bsp19I CCATGG 2 cut(s) 2260, 2421
BspACI CCGC 2 cut(s) 1557, 2079
BspANI GGCC 5 cut(s) 1069, 1690, 1879, 2259, 2426
BspCNI CTCAG 1 cut(s) 972
BspDI ATCGAT 1 cut(s) 2370
BspEI TCCGGA 1 cut(s) 58
BspFNI CGCG 1 cut(s) 2492
BspLI GGNNCC 9 cut(s) 333, 553, 774, 1464, 1565, 1653, 1690, 1733, 2406
BspMAI CTGCAG 2 cut(s) 528, 1866
BspPI GGATC 8 cut(s) 283, 546, 559, 1726, 1739, 1877, 2167, 2272
BspT107I GGYRCC 1 cut(s) 2404
BspTI CTTAAG 1 cut(s) 577
BsrGI TGTACA 1 cut(s) 1452
BsrI ACTGG 2 cut(s) 51, 2192
BssECI CCNNGG 9 cut(s) 335, 756, 1050, 1417, 1597, 1637, 1735, 2260, 2421
BssSI CACGAG 1 cut(s) 979
BssT1I CCWWGG 2 cut(s) 2260, 2421
Bst2BI CACGAG 1 cut(s) 979
Bst2UI CCWGG 5 cut(s) 336, 1598, 1638, 2039, 2157
Bst4CI ACNGT 9 cut(s) 65, 211, 1019, 1321, 1363, 1563, 1708, 1975, 2074
Bst6I CTCTTC 2 cut(s) 457, 2055
BstAFI CTTAAG 1 cut(s) 577
BstAUI TGTACA 1 cut(s) 1452
BstBAI YACGTR 1 cut(s) 2120
BstC8I GCNNGC 4 cut(s) 145, 1881, 2324, 2386
BstDEI CTNAG 8 cut(s) 696, 728, 844, 912, 959, 1133, 1201, 2216
BstDSI CCRYGG 3 cut(s) 1417, 2260, 2421
BstENI CCTNNNNNAGG 1 cut(s) 1726
BstF5I GGATG 3 cut(s) 496, 1593, 2215
BstFNI CGCG 1 cut(s) 2492
BstHHI GCGC 2 cut(s) 1404, 2492
BstMWI GCNNNNNNNGC 5 cut(s) 383, 1300, 1309, 2293, 2352
BstNI CCWGG 5 cut(s) 336, 1598, 1638, 2039, 2157
BstNSI RCATGY 1 cut(s) 2388
BstPAI GACNNNNGTC 1 cut(s) 1128
BstSCI CCNGG 6 cut(s) 334, 756, 1596, 1636, 2037, 2155
BstSFI CTRYAG 3 cut(s) 524, 1320, 1862
BstSLI GKGCMC 2 cut(s) 1329, 1692
BstUI CGCG 1 cut(s) 2492
BstV1I GCAGC 4 cut(s) 538, 681, 2139, 2358
BstV2I GAAGAC 1 cut(s) 1580
BstX2I RGATCY 5 cut(s) 551, 1204, 1731, 1869, 2264
BstYI RGATCY 5 cut(s) 551, 1204, 1731, 1869, 2264
Bsu15I ATCGAT 1 cut(s) 2370
BsuRI GGCC 5 cut(s) 1069, 1690, 1879, 2259, 2426
BsuTUI ATCGAT 1 cut(s) 2370
BtgI CCRYGG 3 cut(s) 1417, 2260, 2421
BtgZI GCGATG 3 cut(s) 1791, 1977, 2110
BtsCI GGATG 3 cut(s) 496, 1593, 2215
BtsI GCAGTG 3 cut(s) 1041, 1871, 2383
BtsIMutI CAGTG 4 cut(s) 1041, 1455, 1871, 2383
Cac8I GCNNGC 4 cut(s) 145, 1881, 2324, 2386
CaiI CAGNNNCTG 1 cut(s) 1603
CfoI GCGC 2 cut(s) 1404, 2492
Cfr13I GGNCC 4 cut(s) 772, 1688, 1689, 1877
ClaI ATCGAT 1 cut(s) 2370
CseI GACGC 1 cut(s) 1574
Csp6I GTAC 6 cut(s) 1390, 1453, 1546, 1715, 2047, 2405
CviQI GTAC 6 cut(s) 1390, 1453, 1546, 1715, 2047, 2405
DdeI CTNAG 8 cut(s) 696, 728, 844, 912, 959, 1133, 1201, 2216
DraIII CACNNNGTG 1 cut(s) 1194
EaeI YGGCCR 2 cut(s) 1067, 2424
Eam1104I CTCTTC 2 cut(s) 457, 2055
EarI CTCTTC 2 cut(s) 457, 2055
Eco130I CCWWGG 2 cut(s) 2260, 2421
Eco24I GRGCYC 1 cut(s) 1692
Eco32I GATATC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 772
Eco72I CACGTG 1 cut(s) 2120
EcoNI CCTNNNNNAGG 1 cut(s) 1726
EcoO109I RGGNCCY 2 cut(s) 1688, 1689
EcoRI GAATTC 2 cut(s) 219, 1893
EcoRII CCWGG 5 cut(s) 334, 1596, 1636, 2037, 2155
EcoRV GATATC 1 cut(s) 172
EcoT14I CCWWGG 2 cut(s) 2260, 2421
EcoT22I ATGCAT 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 1692
ErhI CCWWGG 2 cut(s) 2260, 2421
FaqI GGGAC 4 cut(s) 758, 864, 1614, 2286
FauI CCCGC 1 cut(s) 1564
FbaI TGATCA 2 cut(s) 1491, 1906
FblI GTMKAC 2 cut(s) 1122, 1267
Fnu4HI GCNGC 4 cut(s) 527, 670, 2153, 2347
FokI GGATG 3 cut(s) 503, 1580, 2222
FriOI GRGCYC 1 cut(s) 1692
Fsp4HI GCNGC 4 cut(s) 527, 670, 2153, 2347
FspBI CTAG 4 cut(s) 188, 782, 1395, 1433
GlaI GCGC 2 cut(s) 1403, 2491
GluI GCNGC 4 cut(s) 527, 670, 2153, 2347
HaeIII GGCC 5 cut(s) 1069, 1690, 1879, 2259, 2426
HapII CCGG 3 cut(s) 59, 758, 2130
HgaI GACGC 1 cut(s) 1574
HhaI GCGC 2 cut(s) 1404, 2492
Hin6I GCGC 2 cut(s) 1402, 2490
HinP1I GCGC 2 cut(s) 1402, 2490
HindIII AAGCTT 3 cut(s) 574, 811, 893
HpaII CCGG 3 cut(s) 59, 758, 2130
HphI GGTGA 5 cut(s) 739, 1163, 1486, 2068, 2080
Hpy166II GTNNAC 5 cut(s) 1123, 1268, 1390, 1453, 2047
Hpy188I TCNGA 7 cut(s) 158, 570, 625, 999, 1736, 1796, 1892
Hpy8I GTNNAC 5 cut(s) 1123, 1268, 1390, 1453, 2047
HpyAV CCTTC 8 cut(s) 143, 407, 601, 682, 1949, 2059, 2186, 2199
HpyCH4III ACNGT 9 cut(s) 65, 211, 1019, 1321, 1363, 1563, 1708, 1975, 2074
HpyCH4IV ACGT 4 cut(s) 343, 429, 1190, 2119
HpyF10VI GCNNNNNNNGC 5 cut(s) 383, 1300, 1309, 2293, 2352
HpyF3I CTNAG 8 cut(s) 696, 728, 844, 912, 959, 1133, 1201, 2216
HpySE526I ACGT 4 cut(s) 343, 429, 1190, 2119
HspAI GCGC 2 cut(s) 1402, 2490
Kpn2I TCCGGA 1 cut(s) 58
KpnI GGTACC 1 cut(s) 2408
Ksp22I TGATCA 2 cut(s) 1491, 1906
LmnI GCTCC 3 cut(s) 331, 1409, 1462
Lsp1109I GCAGC 4 cut(s) 538, 681, 2139, 2358
LweI GCATC 7 cut(s) 181, 267, 289, 367, 481, 495, 2432
MaeI CTAG 4 cut(s) 188, 782, 1395, 1433
MaeII ACGT 4 cut(s) 343, 429, 1190, 2119
MfeI CAATTG 2 cut(s) 408, 2356
MflI RGATCY 5 cut(s) 551, 1204, 1731, 1869, 2264
MhlI GDGCHC 2 cut(s) 1329, 1692
MlsI TGGCCA 2 cut(s) 1069, 2426
MluNI TGGCCA 2 cut(s) 1069, 2426
MlyI GAGTC 5 cut(s) 246, 1138, 1263, 1454, 1658
MmeI TCCRAC 4 cut(s) 196, 593, 1335, 2375
Mox20I TGGCCA 2 cut(s) 1069, 2426
Mph1103I ATGCAT 1 cut(s) 282
MroI TCCGGA 1 cut(s) 58
MroXI GAANNNNTTC 3 cut(s) 54, 564, 1748
MscI TGGCCA 2 cut(s) 1069, 2426
MseI TTAA 7 cut(s) 515, 578, 798, 888, 1379, 1925, 2277
MslI CAYNNNNRTG 1 cut(s) 738
Msp20I TGGCCA 2 cut(s) 1069, 2426
MspA1I CMGCKG 1 cut(s) 2287
MspCI CTTAAG 1 cut(s) 577
MspI CCGG 3 cut(s) 59, 758, 2130
MspR9I CCNGG 6 cut(s) 336, 758, 1598, 1638, 2039, 2157
MunI CAATTG 2 cut(s) 408, 2356
Mva1269I GAATGC 3 cut(s) 1404, 1418, 2324
MvaI CCWGG 5 cut(s) 336, 1598, 1638, 2039, 2157
MvnI CGCG 1 cut(s) 2492
MwoI GCNNNNNNNGC 5 cut(s) 383, 1300, 1309, 2293, 2352
NciI CCSGG 1 cut(s) 758
NcoI CCATGG 2 cut(s) 2260, 2421
NlaIV GGNNCC 9 cut(s) 333, 553, 774, 1464, 1565, 1653, 1690, 1733, 2406
NmuCI GTSAC 5 cut(s) 293, 1057, 2068, 2074, 2139
NsiI ATGCAT 1 cut(s) 282
NspI RCATGY 1 cut(s) 2388
PaeI GCATGC 1 cut(s) 2388
PctI GAATGC 3 cut(s) 1404, 1418, 2324
PdmI GAANNNNTTC 3 cut(s) 54, 564, 1748
PfeI GAWTC 8 cut(s) 98, 323, 368, 718, 793, 830, 992, 1781
PkrI GCNGC 4 cut(s) 528, 671, 2154, 2348
PleI GAGTC 5 cut(s) 246, 1137, 1263, 1453, 1658
PmaCI CACGTG 1 cut(s) 2120
PmlI CACGTG 1 cut(s) 2120
PpsI GAGTC 5 cut(s) 246, 1137, 1263, 1453, 1658
Ppu21I YACGTR 1 cut(s) 2120
PshAI GACNNNNGTC 1 cut(s) 1128
Psp6I CCWGG 5 cut(s) 334, 1596, 1636, 2037, 2155
PspCI CACGTG 1 cut(s) 2120
PspGI CCWGG 5 cut(s) 334, 1596, 1636, 2037, 2155
PspN4I GGNNCC 9 cut(s) 333, 553, 774, 1464, 1565, 1653, 1690, 1733, 2406
PspOMI GGGCCC 1 cut(s) 1688
PspPI GGNCC 4 cut(s) 772, 1688, 1689, 1877
PstI CTGCAG 2 cut(s) 528, 1866
PstNI CAGNNNCTG 1 cut(s) 1603
PsuI RGATCY 5 cut(s) 551, 1204, 1731, 1869, 2264
PvuII CAGCTG 1 cut(s) 2287
RsaI GTAC 6 cut(s) 1391, 1454, 1547, 1716, 2048, 2406
RsaNI GTAC 6 cut(s) 1390, 1453, 1546, 1715, 2047, 2405
RseI CAYNNNNRTG 1 cut(s) 738
SaqAI TTAA 7 cut(s) 515, 578, 798, 888, 1379, 1925, 2277
SatI GCNGC 4 cut(s) 527, 670, 2153, 2347
Sau96I GGNCC 4 cut(s) 772, 1688, 1689, 1877
SchI GAGTC 5 cut(s) 246, 1138, 1263, 1454, 1658
ScrFI CCNGG 6 cut(s) 336, 758, 1598, 1638, 2039, 2157
SduI GDGCHC 2 cut(s) 1329, 1692
SfaNI GCATC 7 cut(s) 181, 267, 289, 367, 481, 495, 2432
SfcI CTRYAG 3 cut(s) 524, 1320, 1862
SinI GGWCC 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 738
SmlI CTYRAG 4 cut(s) 577, 952, 1126, 2437
SmoI CTYRAG 4 cut(s) 577, 952, 1126, 2437
SphI GCATGC 1 cut(s) 2388
SsiI CCGC 2 cut(s) 1557, 2079
SspI AATATT 4 cut(s) 875, 907, 1523, 1620
SspMI CTAG 4 cut(s) 188, 782, 1395, 1433
StyD4I CCNGG 6 cut(s) 334, 756, 1596, 1636, 2037, 2155
StyI CCWWGG 2 cut(s) 2260, 2421
TaaI ACNGT 9 cut(s) 65, 211, 1019, 1321, 1363, 1563, 1708, 1975, 2074
TaiI ACGT 4 cut(s) 346, 432, 1193, 2122
TaqI TCGA 4 cut(s) 255, 1410, 2308, 2370
TatI WGTACW 5 cut(s) 1389, 1452, 1545, 1714, 2046
TfiI GAWTC 8 cut(s) 98, 323, 368, 718, 793, 830, 992, 1781
Tru1I TTAA 7 cut(s) 515, 578, 798, 888, 1379, 1925, 2277
Tru9I TTAA 7 cut(s) 515, 578, 798, 888, 1379, 1925, 2277
TscAI CASTG 4 cut(s) 1048, 1455, 1871, 2383
TseFI GTSAC 5 cut(s) 293, 1057, 2068, 2074, 2139
TseI GCWGC 4 cut(s) 526, 669, 2152, 2346
Tsp45I GTSAC 5 cut(s) 293, 1057, 2068, 2074, 2139
TspGWI ACGGA 2 cut(s) 281, 503
TspRI CASTG 4 cut(s) 1048, 1455, 1871, 2383
Vha464I CTTAAG 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 772
XagI CCTNNNNNAGG 1 cut(s) 1726
XbaI TCTAGA 2 cut(s) 781, 1394
XceI RCATGY 1 cut(s) 2388
XcmI CCANNNNNNNNNTGG 1 cut(s) 833
XmiI GTMKAC 2 cut(s) 1122, 1267
XmnI GAANNNNTTC 3 cut(s) 54, 564, 1748
XspI CTAG 4 cut(s) 188, 782, 1395, 1433
Zsp2I ATGCAT 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.