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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 411 |
| Acc36I | ACCTGC | 1 cut(s) | 411 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 157 |
| AccI | GTMKAC | 2 cut(s) | 368, 852 |
| AccII | CGCG | 2 cut(s) | 105, 930 |
| AccIII | TCCGGA | 1 cut(s) | 767 |
| AciI | CCGC | 4 cut(s) | 103, 429, 921, 1136 |
| AclWI | GGATC | 4 cut(s) | 335, 697, 778, 932 |
| AcsI | RAATTY | 1 cut(s) | 864 |
| AcuI | CTGAAG | 2 cut(s) | 45, 915 |
| AfaI | GTAC | 3 cut(s) | 224, 670, 1011 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 157, 435, 540, 947 |
| AflII | CTTAAG | 1 cut(s) | 1160 |
| AgeI | ACCGGT | 1 cut(s) | 253 |
| AgsI | TTSAA | 4 cut(s) | 265, 396, 742, 1183 |
| AjnI | CCWGG | 3 cut(s) | 186, 981, 1125 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 65, 97, 888, 920 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 762, 794 |
| AluBI | AGCT | 1 cut(s) | 530 |
| AluI | AGCT | 1 cut(s) | 530 |
| Alw21I | GWGCWC | 1 cut(s) | 428 |
| Alw26I | GTCTC | 3 cut(s) | 52, 70, 495 |
| AlwI | GGATC | 4 cut(s) | 335, 697, 778, 932 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 68, 893 |
| Aor13HI | TCCGGA | 1 cut(s) | 767 |
| AoxI | GGCC | 3 cut(s) | 189, 267, 1128 |
| ApeKI | GCWGC | 2 cut(s) | 5, 1042 |
| ApoI | RAATTY | 1 cut(s) | 864 |
| AsiGI | ACCGGT | 1 cut(s) | 253 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 550, 688 |
| AspLEI | GCGC | 3 cut(s) | 930, 1025, 1102 |
| AspS9I | GGNCC | 2 cut(s) | 994, 1129 |
| AsuC2I | CCSGG | 1 cut(s) | 541 |
| AvaII | GGWCC | 1 cut(s) | 994 |
| Bbv12I | GWGCWC | 1 cut(s) | 428 |
| BbvCI | CCTCAGC | 1 cut(s) | 177 |
| BbvI | GCAGC | 2 cut(s) | 17, 1054 |
| BccI | CCATC | 3 cut(s) | 154, 587, 674 |
| BceAI | ACGGC | 1 cut(s) | 494 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 898, 932 |
| BciT130I | CCWGG | 3 cut(s) | 188, 983, 1127 |
| BcnI | CCSGG | 1 cut(s) | 541 |
| BcoDI | GTCTC | 3 cut(s) | 52, 70, 495 |
| BfaI | CTAG | 1 cut(s) | 527 |
| BfmI | CTRYAG | 2 cut(s) | 135, 675 |
| BfoI | RGCGCY | 1 cut(s) | 1026 |
| BfrI | CTTAAG | 1 cut(s) | 1160 |
| BfuAI | ACCTGC | 1 cut(s) | 411 |
| BisI | GCNGC | 2 cut(s) | 6, 1043 |
| BlsI | GCNGC | 2 cut(s) | 7, 1044 |
| Bme1390I | CCNGG | 4 cut(s) | 188, 541, 983, 1127 |
| Bme18I | GGWCC | 1 cut(s) | 994 |
| BmgT120I | GGNCC | 2 cut(s) | 994, 1129 |
| BmiI | GGNNCC | 3 cut(s) | 598, 996, 1124 |
| BmrFI | CCNGG | 4 cut(s) | 188, 541, 983, 1127 |
| Bpu10I | CCTNAGC | 2 cut(s) | 10, 177 |
| BpuMI | CCSGG | 1 cut(s) | 541 |
| Bsa29I | ATCGAT | 1 cut(s) | 936 |
| BsaJI | CCNNGG | 5 cut(s) | 214, 432, 510, 714, 981 |
| BsaWI | WCCGGW | 2 cut(s) | 253, 767 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 157, 435, 540, 947 |
| Bse118I | RCCGGY | 3 cut(s) | 253, 281, 1019 |
| Bse3DI | GCAATG | 2 cut(s) | 270, 504 |
| BseAI | TCCGGA | 1 cut(s) | 767 |
| BseBI | CCWGG | 3 cut(s) | 188, 983, 1127 |
| BseCI | ATCGAT | 1 cut(s) | 936 |
| BseDI | CCNNGG | 5 cut(s) | 214, 432, 510, 714, 981 |
| BseGI | GGATG | 2 cut(s) | 146, 875 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 157, 435, 540, 947 |
| BseMI | GCAATG | 2 cut(s) | 270, 504 |
| BseMII | CTCAG | 1 cut(s) | 191 |
| BseXI | GCAGC | 2 cut(s) | 17, 1054 |
| BsgI | GTGCAG | 1 cut(s) | 1180 |
| Bsh1236I | CGCG | 2 cut(s) | 105, 930 |
| BshFI | GGCC | 3 cut(s) | 191, 269, 1130 |
| BshTI | ACCGGT | 1 cut(s) | 253 |
| BshVI | ATCGAT | 1 cut(s) | 936 |
| BsiHKAI | GWGCWC | 1 cut(s) | 428 |
| BsiSI | CCGG | 6 cut(s) | 254, 282, 540, 768, 1020, 1097 |
| BslFI | GGGAC | 1 cut(s) | 980 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 157, 435, 540, 947 |
| BsmAI | GTCTC | 3 cut(s) | 52, 70, 495 |
| BsmFI | GGGAC | 1 cut(s) | 980 |
| BsmI | GAATGC | 1 cut(s) | 169 |
| BsnI | GGCC | 3 cut(s) | 191, 269, 1130 |
| Bsp1286I | GDGCHC | 1 cut(s) | 428 |
| Bsp13I | TCCGGA | 1 cut(s) | 767 |
| Bsp1407I | TGTACA | 1 cut(s) | 1009 |
| Bsp143I | GATC | 8 cut(s) | 340, 481, 702, 770, 802, 933, 937, 1113 |
| BspACI | CCGC | 4 cut(s) | 103, 429, 921, 1136 |
| BspANI | GGCC | 3 cut(s) | 191, 269, 1130 |
| BspCNI | CTCAG | 1 cut(s) | 190 |
| BspDI | ATCGAT | 1 cut(s) | 936 |
| BspEI | TCCGGA | 1 cut(s) | 767 |
| BspFNI | CGCG | 2 cut(s) | 105, 930 |
| BspLI | GGNNCC | 3 cut(s) | 598, 996, 1124 |
| BspMAI | CTGCAG | 1 cut(s) | 139 |
| BspMI | ACCTGC | 1 cut(s) | 411 |
| BspPI | GGATC | 4 cut(s) | 335, 697, 778, 932 |
| BspQI | GCTCTTC | 1 cut(s) | 397 |
| BspTI | CTTAAG | 1 cut(s) | 1160 |
| BsrDI | GCAATG | 2 cut(s) | 270, 504 |
| BsrFI | RCCGGY | 3 cut(s) | 253, 281, 1019 |
| BsrGI | TGTACA | 1 cut(s) | 1009 |
| BssAI | RCCGGY | 3 cut(s) | 253, 281, 1019 |
| BssECI | CCNNGG | 5 cut(s) | 214, 432, 510, 714, 981 |
| BssMI | GATC | 8 cut(s) | 340, 481, 702, 770, 802, 933, 937, 1113 |
| Bst2UI | CCWGG | 3 cut(s) | 188, 983, 1127 |
| Bst4CI | ACNGT | 4 cut(s) | 215, 382, 1009, 1078 |
| Bst6I | CTCTTC | 2 cut(s) | 64, 397 |
| BstAFI | CTTAAG | 1 cut(s) | 1160 |
| BstAUI | TGTACA | 1 cut(s) | 1009 |
| BstC8I | GCNNGC | 2 cut(s) | 311, 457 |
| BstDEI | CTNAG | 2 cut(s) | 10, 177 |
| BstDSI | CCRYGG | 2 cut(s) | 214, 510 |
| BstEII | GGTNACC | 1 cut(s) | 722 |
| BstF5I | GGATG | 2 cut(s) | 146, 875 |
| BstFNI | CGCG | 2 cut(s) | 105, 930 |
| BstH2I | RGCGCY | 1 cut(s) | 1026 |
| BstHHI | GCGC | 3 cut(s) | 930, 1025, 1102 |
| BstKTI | GATC | 8 cut(s) | 343, 484, 705, 773, 805, 936, 940, 1116 |
| BstMAI | GTCTC | 3 cut(s) | 52, 70, 495 |
| BstMBI | GATC | 8 cut(s) | 340, 481, 702, 770, 802, 933, 937, 1113 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 11, 20, 310, 927 |
| BstNI | CCWGG | 3 cut(s) | 188, 983, 1127 |
| BstPI | GGTNACC | 1 cut(s) | 722 |
| BstSCI | CCNGG | 4 cut(s) | 186, 539, 981, 1125 |
| BstSFI | CTRYAG | 2 cut(s) | 135, 675 |
| BstUI | CGCG | 2 cut(s) | 105, 930 |
| BstV1I | GCAGC | 2 cut(s) | 17, 1054 |
| BstX2I | RGATCY | 1 cut(s) | 770 |
| BstYI | RGATCY | 1 cut(s) | 770 |
| Bsu15I | ATCGAT | 1 cut(s) | 936 |
| BsuRI | GGCC | 3 cut(s) | 191, 269, 1130 |
| BsuTUI | ATCGAT | 1 cut(s) | 936 |
| BtgI | CCRYGG | 2 cut(s) | 214, 510 |
| BtgZI | GCGATG | 1 cut(s) | 1083 |
| BtsCI | GGATG | 2 cut(s) | 146, 875 |
| BtsI | GCAGTG | 2 cut(s) | 292, 311 |
| BtsIMutI | CAGTG | 2 cut(s) | 292, 311 |
| BveI | ACCTGC | 1 cut(s) | 411 |
| Cac8I | GCNNGC | 2 cut(s) | 311, 457 |
| CaiI | CAGNNNCTG | 2 cut(s) | 68, 893 |
| CfoI | GCGC | 3 cut(s) | 930, 1025, 1102 |
| Cfr10I | RCCGGY | 3 cut(s) | 253, 281, 1019 |
| Cfr13I | GGNCC | 2 cut(s) | 994, 1129 |
| ClaI | ATCGAT | 1 cut(s) | 936 |
| CseI | GACGC | 3 cut(s) | 310, 838, 848 |
| Csp6I | GTAC | 3 cut(s) | 223, 669, 1010 |
| CspAI | ACCGGT | 1 cut(s) | 253 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 196, 231, 775, 810 |
| CviAII | CATG | 1 cut(s) | 229 |
| CviQI | GTAC | 3 cut(s) | 223, 669, 1010 |
| DdeI | CTNAG | 2 cut(s) | 10, 177 |
| DpnI | GATC | 8 cut(s) | 342, 483, 704, 772, 804, 935, 939, 1115 |
| DpnII | GATC | 8 cut(s) | 340, 481, 702, 770, 802, 933, 937, 1113 |
| Eam1104I | CTCTTC | 2 cut(s) | 64, 397 |
| EarI | CTCTTC | 2 cut(s) | 64, 397 |
| EciI | GGCGGA | 1 cut(s) | 418 |
| Eco47I | GGWCC | 1 cut(s) | 994 |
| Eco57I | CTGAAG | 2 cut(s) | 45, 915 |
| Eco91I | GGTNACC | 1 cut(s) | 722 |
| EcoO109I | RGGNCCY | 1 cut(s) | 994 |
| EcoO65I | GGTNACC | 1 cut(s) | 722 |
| EcoRII | CCWGG | 3 cut(s) | 186, 981, 1125 |
| FaeI | CATG | 1 cut(s) | 232 |
| FaiI | YATR | 9 cut(s) | 203, 230, 477, 623, 677, 694, 845, 1119, 1155 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 728, 760 |
| FaqI | GGGAC | 1 cut(s) | 980 |
| FatI | CATG | 1 cut(s) | 228 |
| FauI | CCCGC | 2 cut(s) | 914, 1129 |
| FblI | GTMKAC | 2 cut(s) | 368, 852 |
| Fnu4HI | GCNGC | 2 cut(s) | 6, 1043 |
| FokI | GGATG | 2 cut(s) | 133, 862 |
| Fsp4HI | GCNGC | 2 cut(s) | 6, 1043 |
| FspBI | CTAG | 1 cut(s) | 527 |
| GlaI | GCGC | 3 cut(s) | 929, 1024, 1101 |
| GluI | GCNGC | 2 cut(s) | 6, 1043 |
| HaeII | RGCGCY | 1 cut(s) | 1026 |
| HaeIII | GGCC | 3 cut(s) | 191, 269, 1130 |
| HapII | CCGG | 6 cut(s) | 254, 282, 540, 768, 1020, 1097 |
| HgaI | GACGC | 3 cut(s) | 310, 838, 848 |
| HhaI | GCGC | 3 cut(s) | 930, 1025, 1102 |
| Hin1II | CATG | 1 cut(s) | 232 |
| Hin6I | GCGC | 3 cut(s) | 928, 1023, 1100 |
| HinP1I | GCGC | 3 cut(s) | 928, 1023, 1100 |
| HincII | GTYRAC | 1 cut(s) | 853 |
| HindII | GTYRAC | 1 cut(s) | 853 |
| HinfI | GANTC | 5 cut(s) | 107, 194, 647, 973, 1105 |
| HpaII | CCGG | 6 cut(s) | 254, 282, 540, 768, 1020, 1097 |
| Hpy166II | GTNNAC | 5 cut(s) | 198, 369, 472, 514, 853 |
| Hpy188I | TCNGA | 4 cut(s) | 500, 718, 895, 945 |
| Hpy188III | TCNNGA | 4 cut(s) | 298, 559, 768, 829 |
| Hpy8I | GTNNAC | 5 cut(s) | 198, 369, 472, 514, 853 |
| Hpy99I | CGWCG | 1 cut(s) | 854 |
| HpyAV | CCTTC | 7 cut(s) | 47, 133, 366, 713, 829, 880, 1189 |
| HpyCH4III | ACNGT | 4 cut(s) | 215, 382, 1009, 1078 |
| HpyCH4V | TGCA | 4 cut(s) | 29, 137, 309, 1197 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 11, 20, 310, 927 |
| HpyF3I | CTNAG | 2 cut(s) | 10, 177 |
| Hsp92II | CATG | 1 cut(s) | 232 |
| HspAI | GCGC | 3 cut(s) | 928, 1023, 1100 |
| Kpn2I | TCCGGA | 1 cut(s) | 767 |
| Kzo9I | GATC | 8 cut(s) | 340, 481, 702, 770, 802, 933, 937, 1113 |
| LguI | GCTCTTC | 1 cut(s) | 397 |
| LmnI | GCTCC | 3 cut(s) | 431, 1039, 1099 |
| Lsp1109I | GCAGC | 2 cut(s) | 17, 1054 |
| MaeI | CTAG | 1 cut(s) | 527 |
| MaeIII | GTNAC | 7 cut(s) | 287, 581, 641, 648, 722, 749, 1190 |
| MalI | GATC | 8 cut(s) | 342, 483, 704, 772, 804, 935, 939, 1115 |
| MboI | GATC | 8 cut(s) | 340, 481, 702, 770, 802, 933, 937, 1113 |
| MboII | GAAGA | 5 cut(s) | 51, 384, 814, 817, 908 |
| MfeI | CAATTG | 2 cut(s) | 240, 662 |
| MflI | RGATCY | 1 cut(s) | 770 |
| MhlI | GDGCHC | 1 cut(s) | 428 |
| MluCI | AATT | 6 cut(s) | 52, 96, 240, 662, 864, 882 |
| MlyI | GAGTC | 4 cut(s) | 203, 656, 982, 1114 |
| MmeI | TCCRAC | 1 cut(s) | 1017 |
| MroI | TCCGGA | 1 cut(s) | 767 |
| MroXI | GAANNNNTTC | 2 cut(s) | 550, 688 |
| MseI | TTAA | 5 cut(s) | 413, 521, 966, 990, 1161 |
| MslI | CAYNNNNRTG | 2 cut(s) | 509, 588 |
| MspCI | CTTAAG | 1 cut(s) | 1160 |
| MspI | CCGG | 6 cut(s) | 254, 282, 540, 768, 1020, 1097 |
| MspR9I | CCNGG | 4 cut(s) | 188, 541, 983, 1127 |
| MunI | CAATTG | 2 cut(s) | 240, 662 |
| Mva1269I | GAATGC | 1 cut(s) | 169 |
| MvaI | CCWGG | 3 cut(s) | 188, 983, 1127 |
| MvnI | CGCG | 2 cut(s) | 105, 930 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 11, 20, 310, 927 |
| NciI | CCSGG | 1 cut(s) | 541 |
| NdeII | GATC | 8 cut(s) | 340, 481, 702, 770, 802, 933, 937, 1113 |
| NlaIII | CATG | 1 cut(s) | 232 |
| NlaIV | GGNNCC | 3 cut(s) | 598, 996, 1124 |
| NmeAIII | GCCGAG | 2 cut(s) | 217, 457 |
| NmuCI | GTSAC | 7 cut(s) | 287, 581, 641, 648, 722, 749, 1190 |
| PaqCI | CACCTGC | 1 cut(s) | 411 |
| PciSI | GCTCTTC | 1 cut(s) | 397 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 846 |
| PctI | GAATGC | 1 cut(s) | 169 |
| PdmI | GAANNNNTTC | 2 cut(s) | 550, 688 |
| PfeI | GAWTC | 1 cut(s) | 107 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 157 |
| PinAI | ACCGGT | 1 cut(s) | 253 |
| PkrI | GCNGC | 2 cut(s) | 7, 1044 |
| PleI | GAGTC | 4 cut(s) | 202, 655, 981, 1113 |
| PpsI | GAGTC | 4 cut(s) | 202, 655, 981, 1113 |
| PpuMI | RGGWCCY | 1 cut(s) | 994 |
| Psp5II | RGGWCCY | 1 cut(s) | 994 |
| Psp6I | CCWGG | 3 cut(s) | 186, 981, 1125 |
| PspEI | GGTNACC | 1 cut(s) | 722 |
| PspGI | CCWGG | 3 cut(s) | 186, 981, 1125 |
| PspN4I | GGNNCC | 3 cut(s) | 598, 996, 1124 |
| PspPI | GGNCC | 2 cut(s) | 994, 1129 |
| PspPPI | RGGWCCY | 1 cut(s) | 994 |
| PstI | CTGCAG | 1 cut(s) | 139 |
| PstNI | CAGNNNCTG | 2 cut(s) | 68, 893 |
| PsuI | RGATCY | 1 cut(s) | 770 |
| RsaI | GTAC | 3 cut(s) | 224, 670, 1011 |
| RsaNI | GTAC | 3 cut(s) | 223, 669, 1010 |
| RseI | CAYNNNNRTG | 2 cut(s) | 509, 588 |
| SalI | GTCGAC | 1 cut(s) | 851 |
| SapI | GCTCTTC | 1 cut(s) | 397 |
| SaqAI | TTAA | 5 cut(s) | 413, 521, 966, 990, 1161 |
| SatI | GCNGC | 2 cut(s) | 6, 1043 |
| Sau3AI | GATC | 8 cut(s) | 340, 481, 702, 770, 802, 933, 937, 1113 |
| Sau96I | GGNCC | 2 cut(s) | 994, 1129 |
| SchI | GAGTC | 4 cut(s) | 203, 656, 982, 1114 |
| ScrFI | CCNGG | 4 cut(s) | 188, 541, 983, 1127 |
| SduI | GDGCHC | 1 cut(s) | 428 |
| SfcI | CTRYAG | 2 cut(s) | 135, 675 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1019 |
| SinI | GGWCC | 1 cut(s) | 994 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 509, 588 |
| SmlI | CTYRAG | 1 cut(s) | 1160 |
| SmoI | CTYRAG | 1 cut(s) | 1160 |
| Sse9I | AATT | 6 cut(s) | 52, 96, 240, 662, 864, 882 |
| SsiI | CCGC | 4 cut(s) | 103, 429, 921, 1136 |
| SspI | AATATT | 1 cut(s) | 1003 |
| SspMI | CTAG | 1 cut(s) | 527 |
| StyD4I | CCNGG | 4 cut(s) | 186, 539, 981, 1125 |
| TaaI | ACNGT | 4 cut(s) | 215, 382, 1009, 1078 |
| TaqI | TCGA | 4 cut(s) | 351, 852, 936, 1112 |
| TasI | AATT | 6 cut(s) | 52, 96, 240, 662, 864, 882 |
| TatI | WGTACW | 1 cut(s) | 1009 |
| TfiI | GAWTC | 1 cut(s) | 107 |
| Tru1I | TTAA | 5 cut(s) | 413, 521, 966, 990, 1161 |
| Tru9I | TTAA | 5 cut(s) | 413, 521, 966, 990, 1161 |
| TscAI | CASTG | 2 cut(s) | 292, 311 |
| TseFI | GTSAC | 7 cut(s) | 287, 581, 641, 648, 722, 749, 1190 |
| TseI | GCWGC | 2 cut(s) | 5, 1042 |
| Tsp45I | GTSAC | 7 cut(s) | 287, 581, 641, 648, 722, 749, 1190 |
| TspDTI | ATGAA | 1 cut(s) | 681 |
| TspRI | CASTG | 2 cut(s) | 292, 311 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 157 |
| Vha464I | CTTAAG | 1 cut(s) | 1160 |
| VpaK11BI | GGWCC | 1 cut(s) | 994 |
| XapI | RAATTY | 1 cut(s) | 864 |
| XmiI | GTMKAC | 2 cut(s) | 368, 852 |
| XmnI | GAANNNNTTC | 2 cut(s) | 550, 688 |
| XspI | CTAG | 1 cut(s) | 527 |