RchiOBHm_Chr1g0348431

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000036
Physical Location & Seq
Forward (+)
41312055 .. 41318457
6403 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57447

Sequence Viewer

Length: 3624 bp
ATGGATCTTGTTAGCACATCATCAGCTCAACAAGCTTCGTCTTCCAGTATTTCTCCTAGTCAATTCACATATAAAGTGTTCTTGAGTTTCAGAGGCACAGACACTCGCAAGGGTTTCACCGATCATCTCTACACAGCCTTGACCAACGCAGGAATCTGCACTTTCCGAGACGGCGATGAACTCCCGGTAGGAGAAGAGATCAAACCAGAATTAGAGAGAGCCATCCAGCACTCGCGATGTTCTGTTATTGTGTTTTCAAAAGACTACGCGTCGTCCGGATGGTGCCTGGACGAGCTTGTGACGATCCTTCAATGCAGAAAGAGGTCCAATCAGTTCGTTTTACCGATCTTCTATGACGTCGACCCATCCCAATTGGGGGAGCAGACGGGGAGTCTTGGAGAGGCGTTTATCACACACCAAAAGAATGAAACAATGGAAAAGTTGATTCGATGGAAGGAAGCACTTACAGAAGTTGCGGCTCTAGCAGGCATGGTGTTAAAGAATCAAGCTGACGGGCACGAGTCAAAATTTATTGAGAAAATTGTTAAGGTGATTGAAGGCAAGTTAAGTCGAGTTCCCTTATCTGTTCCCGCTTACCTGGTTGGAATTGATTCTCGAGTAAAAAGCATCAATTTGTGGTTACAGGATGGATCATCCAGTACTGGTATATTTGGAATTTGTGGAATTGGTGGAATAGGAAAGACAACTATTGCACAAATTGTTTATAATTCAAATTCTGAGAGATTTGAAAGAAGATGCTTTCTTGAAAATATCGGAGAGATTTTAAAACAGCCAAATGGCTTTGTTCAAATAGAGAAACAGCTTCTTTCCAGTATCTTGAATGGGAGAAAAGTGGATATAAGCTGTCCTAGCGAGGGAATGAGTAAAATAAAAGATGCCATAAGTTCTAAAAAAGTTCTTCTTGTTCTCGATGATGTGGATCACAAGGGCCTAGTTGATGCAATACTTGAGATGAAAGGCTGCTTTTGTTCTGGAAGTAAAATTATAATAACAACAAGGAATGCAGGATTGCTCAAGGGTCATCGAGATATTAAGGTGCATAATGTTGAAACTTTGAACCACATTGAATCATCAGACCTCTTCAGTAGGCATGCTTTTGGGCTCAACCACCCTGTTGAAAGTTACGTGAAACTTTCAGAGAGGGTAGTGCACCAATGCGAAGGACTTCCGTTAGCTCTTCAAACATTGGGTTCTTCTCTCTCAGGGCAGACTATAGATGTATGGGAAAGTGCATTAAAGAAATCCAAAACTATACCAAACAGTATAGTAATGAGTAAACTGAGGACGAGCTTCAACTCTTTACAAGATGACCATGATCAGAGTTTATTCCTCCATATTGCATGTTTTTTTATTGGAAAGGACAAGGATATCATTGTCAAAATTCTAGATGCATGTGGTTTCTACACAACCGTTGGCATTCAAAATCTCATTGATAGATATCTGGTTAGAATTGAAGAATGTAATAAGGTGAATATGCATCACATGATTCGTGACATGGGGAGAGAAATTGTTCGTCTAGAATCAAAAGATCCTGAGAAACGTAGTAGGCTATGGCATCACAAGGACTCTCTTAATGTACTAAGAGAAAAGACTGGTTCAAATGCAATTGAAGGCCTTGTCCTGAACATGCCTATGAACTCTGCACATGGTCCTTCAAGAAATTCAGATATGGTGGTCTTGGATGCCAATGCATTTTCAATGATGAACAAACTCAGATTCCTTCATCTTGGTCATGTACAACTCAATGAAAGTTATGAAAAGTTTCCTAAAGGATTGAGATGGTTGTGTTGGCGCGAATGTCCTTTGAATTTAATACCGAGTGATTTTCCTATGGAGATGATGGTTGTTATTGAAATGTCCTATAGTAGATTGAGACAAGTGTGGAAGGGAGCAAAGCGTCTTCCATCATTGAAGATCTTTGATCTCAGCCATTCCTCAAACCTTACTGAAACTGGTGACTTCTCATTGGTCCAGAATCTGGAGAGACTTACTCTTAAACATTGTATAAGCTTAGTTGATGTCCATGAATCCGTAGGAAAGCTAGAGAAACTTGTTTACTTGAATTTGAAAGATTGCATCAATATCACGAAGCTTCCAGAGAACATTCATATGTTAAAATCTCTTGAAACACTTATTATATCAGGTTGTTCAAATCTTAGCGAGTTTCCAATGGAGATTAGAAACATGGAATCTCTAAAAGTGTTTCAAGCATTTGGAGTTCCAATAGATCAGCTAATCACTATCCCTGGGCAGGTAAATACACGATCAAGATTAAGTGTAGATGGTTTATGGGGTTCCTTTTCTGGCTCTATTGTTGAGTTAGATCTTGGAAGCTGCAATCTTTCTGATGATGCTTTTCCTAGTGATTTGGGTAACTTAGCTTCATTGAGGTATTTGGATCTCAGCTACAATCCCATTTGTACCTTGCCAGATTCAATCAGAGGCCTTTGGAAACTCGAGACGCTTACTCTGAGTTACTGTACAAGTCTCAGATCAATTACAGGTCTACCACGAGTAAGAGATCTACTGAGCGCAGACCATTGTGTATCCTTGGAAAAAATAACATTTCAATCACTTTCATGCATACCAAAGAGATTGTCCATATATGGTGACTCCAAACTAGTTGAGATTCAGCACTGGTACAAGTTAAAACCTATTGGAACAGTTGATGTAGAAATGATCAGACTTTTGGGTTTGTCCGACTTGGAATCCATGGAACCCATTCTGATGCACACCCCAGATGCAAAATCCCCGTTGGAAGGGGAATCCATGGAACCCATTCGCATCCAGGGACTGCATGAATATGGTATCTTCAGCACATTCTTTTCGGGAAACGGAAATGAGGTTCCAGGACAGTTCAGCCATACAAGTAAAGGGTGTTCCTCAATATCATTTCCTGTGCCTATACTTCTTAATCACAAGATCCGGGGCTTCAACATATATTCTGTGTATGCATGGACGACAGATGTTACTACTCTATTGCCACCATTAATCATTGAAGTCAATAATAAGAGCACGGGTCTGAAGTGGATCTATGTGCCGTCATGTTACGGCATTCCAAGTGATGGAAAAGACATGATATGGTTAAGCCATTGGAGTTTGGGGAACCAATTCAAAGGTGGCGATGTAGTCACTGTCTCAATATTTACGCCAGATAACTGGCTTCAGGTAAAAGAGTTTGGTGTCCAAGTGGTGCATGAGGAAGAAGAGAAGATGAGTAAGCAACAGTACAGCAGCAGTAGTAGAGATCCTATTTATGATGATCCCGATGCTTGTGGTGTTAATGAGGTGATTGGTAATAGTGGTGGAGTTTTGTCAGAGTTATATCAGGTCAGGCCAGGGACATACTTCCTCTGCGGTGGACCCTTTTTAGAAAATAATAGATACACATTTAACAGATGGAAAAAGGATCGTTACCTCAGTGACATCATAGGAGAGTATGATGAAGAAACAGACAAAGAAGAAAGGAAACAGGGGGATAGGACACTAACAGCAACAGAAGAAGCTGAAGCAGAAAGTGGCAGCAATAAATTCTTCGGTTGCAAGAACCTTGTTATCCATGTGCTTATGTCATTGGTTGAGATTTGTCAAAATATCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1207

Amino Acids

135.36

Weight (kDa)

6.08

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 193 5.3e-52 TIR domain
TIR_2 PF13676 26 - 125 8.2e-16 TIR domain
NB-ARC PF00931 206 - 373 3.5e-17 NB-ARC domain
WHD_ROQ1 PF23282 444 - 514 1.7e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 664 - 742 4.4e-08 Leucine-rich repeat region
LRR_14 PF23598 781 - 840 3.4e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 726, 1007
AatII GACGTC 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 2263
AccB1I GGYRCC 1 cut(s) 282
AccB7I CCANNNNNTGG 2 cut(s) 1999, 3086
AccI GTMKAC 2 cut(s) 360, 2527
AccII CGCG 3 cut(s) 235, 269, 1815
AccIII TCCGGA 1 cut(s) 275
AciI CCGC 3 cut(s) 476, 591, 3378
AcsI RAATTY 8 cut(s) 527, 675, 733, 1401, 1681, 1828, 2083, 3551
AcuI CTGAAG 5 cut(s) 1087, 2818, 3065, 3170, 3549
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 7 cut(s) 661, 1599, 1758, 2443, 2503, 2663, 3251
AfiI CCNNNNNNNGG 7 cut(s) 375, 376, 875, 1999, 2272, 2780, 3086
AflIII ACRYGT 1 cut(s) 267
AhdI GACNNNNNGTC 2 cut(s) 268, 390
AhlI ACTAGT 1 cut(s) 2641
AjnI CCWGG 6 cut(s) 285, 597, 2266, 2808, 2869, 3358
AloI GAACNNNNNNTCC 2 cut(s) 2850, 2882
Alw21I GWGCWC 2 cut(s) 1173, 3038
Alw26I GTCTC 6 cut(s) 162, 1888, 1999, 2474, 2513, 3163
Alw44I GTGCAC 1 cut(s) 1169
AlwNI CAGNNNCTG 2 cut(s) 1999, 2815
Ama87I CYCGRG 2 cut(s) 615, 2477
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 4 cut(s) 949, 1633, 2464, 3356
ApaLI GTGCAC 1 cut(s) 1169
ApeKI GCWGC 4 cut(s) 981, 2355, 3255, 3543
ApoI RAATTY 8 cut(s) 527, 675, 733, 1401, 1681, 1828, 2083, 3551
ArsI GACNNNNNNTTYG 2 cut(s) 2603, 2635
AseI ATTAAT 1 cut(s) 3011
Asp700I GAANNNNTTC 7 cut(s) 610, 1185, 1530, 1782, 2742, 2799, 3131
AspLEI GCGC 2 cut(s) 1815, 2555
AspS9I GGNCC 5 cut(s) 324, 949, 1670, 1990, 3383
AsuC2I CCSGG 2 cut(s) 185, 2948
AsuHPI GGTGA 6 cut(s) 109, 562, 1501, 1988, 2642, 3322
AvaI CYCGRG 2 cut(s) 615, 2477
AvaII GGWCC 4 cut(s) 324, 1670, 1990, 3383
BaeGI GKGCMC 2 cut(s) 519, 1173
BanI GGYRCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 1125
BarI GAAGNNNNNNTAC 2 cut(s) 2386, 2418
BauI CACGAG 2 cut(s) 518, 2532
BbsI GAAGAC 2 cut(s) 33, 1913
Bbv12I GWGCWC 2 cut(s) 1173, 3038
BbvI GCAGC 4 cut(s) 968, 2342, 3267, 3555
BceAI ACGGC 3 cut(s) 187, 3046, 3088
BciT130I CCWGG 6 cut(s) 287, 599, 2268, 2810, 2871, 3360
BciVI GTATCC 1 cut(s) 2578
BclI TGATCA 2 cut(s) 1336, 2700
BcnI CCSGG 2 cut(s) 185, 2948
BcoDI GTCTC 6 cut(s) 162, 1888, 1999, 2474, 2513, 3163
BcuI ACTAGT 1 cut(s) 2641
BfaI CTAG 9 cut(s) 57, 482, 870, 953, 1406, 1538, 2063, 2382, 2642
BfmI CTRYAG 2 cut(s) 1233, 1882
BfuAI ACCTGC 1 cut(s) 2263
BfuI GTATCC 1 cut(s) 2578
BglII AGATCT 3 cut(s) 1935, 2344, 2542
BisI GCNGC 5 cut(s) 477, 982, 2356, 3256, 3544
BlsI GCNGC 5 cut(s) 478, 983, 2357, 3257, 3545
BmcAI AGTACT 1 cut(s) 661
Bme1390I CCNGG 8 cut(s) 185, 287, 599, 2268, 2810, 2871, 2948, 3360
Bme18I GGWCC 4 cut(s) 324, 1670, 1990, 3383
BmeRI GACNNNNNGTC 2 cut(s) 268, 390
BmeT110I CYCGRG 2 cut(s) 615, 2477
BmgT120I GGNCC 5 cut(s) 324, 949, 1670, 1990, 3383
BmiI GGNNCC 7 cut(s) 284, 2317, 2739, 2796, 2868, 3128, 3385
BmrFI CCNGG 8 cut(s) 185, 287, 599, 2268, 2810, 2871, 2948, 3360
BpiI GAAGAC 2 cut(s) 33, 1913
BplI GAGNNNNNCTC 2 cut(s) 1996, 2028
BpmI CTGGAG 1 cut(s) 2021
BpuEI CTTGAG 3 cut(s) 103, 989, 1019
BpuMI CCSGG 2 cut(s) 185, 2948
BsaAI YACGTR 1 cut(s) 1147
BsaBI GATNNNNATC 2 cut(s) 939, 1458
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 8 cut(s) 2266, 2267, 2571, 2733, 2790, 2809, 2947, 3359
BsaWI WCCGGW 1 cut(s) 275
Bsc4I CCNNNNNNNGG 7 cut(s) 375, 376, 875, 1999, 2272, 2780, 3086
Bse1I ACTGG 8 cut(s) 45, 657, 667, 831, 1618, 1978, 2663, 3185
Bse8I GATNNNNATC 2 cut(s) 939, 1458
BseAI TCCGGA 1 cut(s) 275
BseBI CCWGG 6 cut(s) 287, 599, 2268, 2810, 2871, 3360
BseDI CCNNGG 8 cut(s) 2266, 2267, 2571, 2733, 2790, 2809, 2947, 3359
BseGI GGATG 7 cut(s) 222, 284, 365, 652, 653, 1708, 2805
BseJI GATNNNNATC 2 cut(s) 939, 1458
BseLI CCNNNNNNNGG 7 cut(s) 375, 376, 875, 1999, 2272, 2780, 3086
BseNI ACTGG 8 cut(s) 45, 657, 667, 831, 1618, 1978, 2663, 3185
BseSI GKGCMC 2 cut(s) 519, 1173
BseXI GCAGC 4 cut(s) 968, 2342, 3267, 3555
BsgI GTGCAG 2 cut(s) 142, 1647
Bsh1236I CGCG 3 cut(s) 235, 269, 1815
BshFI GGCC 4 cut(s) 951, 1635, 2466, 3358
BshNI GGYRCC 1 cut(s) 282
BsiHKAI GWGCWC 2 cut(s) 1173, 3038
BsiHKCI CYCGRG 2 cut(s) 615, 2477
BsiSI CCGG 3 cut(s) 185, 276, 2947
BslFI GGGAC 2 cut(s) 2826, 3376
BslI CCNNNNNNNGG 7 cut(s) 375, 376, 875, 1999, 2272, 2780, 3086
BsmAI GTCTC 6 cut(s) 162, 1888, 1999, 2474, 2513, 3163
BsmBI CGTCTC 2 cut(s) 162, 2474
BsmFI GGGAC 2 cut(s) 2826, 3376
BsmI GAATGC 3 cut(s) 1027, 1437, 3075
BsnI GGCC 4 cut(s) 951, 1635, 2466, 3358
BsoBI CYCGRG 2 cut(s) 615, 2477
Bsp1286I GDGCHC 4 cut(s) 519, 1125, 1173, 3038
Bsp13I TCCGGA 1 cut(s) 275
Bsp1407I TGTACA 2 cut(s) 1756, 2501
Bsp19I CCATGG 2 cut(s) 2733, 2790
Bsp68I TCGCGA 1 cut(s) 235
BspACI CCGC 3 cut(s) 476, 591, 3378
BspANI GGCC 4 cut(s) 951, 1635, 2466, 3358
BspEI TCCGGA 1 cut(s) 275
BspFNI CGCG 3 cut(s) 235, 269, 1815
BspLI GGNNCC 7 cut(s) 284, 2317, 2739, 2796, 2868, 3128, 3385
BspMI ACCTGC 1 cut(s) 2263
BspQI GCTCTTC 1 cut(s) 1203
BspT107I GGYRCC 1 cut(s) 282
BsrGI TGTACA 2 cut(s) 1756, 2501
BsrI ACTGG 8 cut(s) 45, 657, 667, 831, 1618, 1978, 2663, 3185
BssECI CCNNGG 8 cut(s) 2266, 2267, 2571, 2733, 2790, 2809, 2947, 3359
BssNI GRCGYC 1 cut(s) 357
BssSI CACGAG 2 cut(s) 518, 2532
BssT1I CCWWGG 3 cut(s) 2571, 2733, 2790
Bst2BI CACGAG 2 cut(s) 518, 2532
Bst2UI CCWGG 6 cut(s) 287, 599, 2268, 2810, 2871, 3360
Bst4CI ACNGT 7 cut(s) 1283, 1432, 2501, 2686, 2877, 3157, 3249
Bst6I CTCTTC 4 cut(s) 189, 1106, 1203, 3222
BstACI GRCGYC 1 cut(s) 357
BstAUI TGTACA 2 cut(s) 1756, 2501
BstBAI YACGTR 1 cut(s) 1147
BstC8I GCNNGC 2 cut(s) 487, 1113
BstDSI CCRYGG 2 cut(s) 2733, 2790
BstF5I GGATG 7 cut(s) 222, 284, 365, 652, 653, 1708, 2805
BstFNI CGCG 3 cut(s) 235, 269, 1815
BstHHI GCGC 2 cut(s) 1815, 2555
BstMAI GTCTC 6 cut(s) 162, 1888, 1999, 2474, 2513, 3163
BstMWI GCNNNNNNNGC 3 cut(s) 32, 482, 870
BstNI CCWGG 6 cut(s) 287, 599, 2268, 2810, 2871, 3360
BstNSI RCATGY 4 cut(s) 1115, 1365, 1416, 1651
BstSCI CCNGG 8 cut(s) 183, 285, 597, 2266, 2808, 2869, 2946, 3358
BstSFI CTRYAG 2 cut(s) 1233, 1882
BstSLI GKGCMC 2 cut(s) 519, 1173
BstUI CGCG 3 cut(s) 235, 269, 1815
BstV1I GCAGC 4 cut(s) 968, 2342, 3267, 3555
BstV2I GAAGAC 2 cut(s) 33, 1913
BstX2I RGATCY 9 cut(s) 4, 1549, 1935, 2344, 2419, 2542, 2943, 3051, 3268
BstXI CCANNNNNNTGG 1 cut(s) 3180
BstYI RGATCY 9 cut(s) 4, 1549, 1935, 2344, 2419, 2542, 2943, 3051, 3268
BsuI GTATCC 1 cut(s) 2578
BsuRI GGCC 4 cut(s) 951, 1635, 2466, 3358
BtgI CCRYGG 2 cut(s) 2733, 2790
BtgZI GCGATG 3 cut(s) 189, 250, 3159
BtsCI GGATG 7 cut(s) 222, 284, 365, 652, 653, 1708, 2805
BtsIMutI CAGTG 3 cut(s) 2656, 3153, 3448
BtuMI TCGCGA 1 cut(s) 235
BveI ACCTGC 1 cut(s) 2263
Cac8I GCNNGC 2 cut(s) 487, 1113
CaiI CAGNNNCTG 2 cut(s) 1999, 2815
CfoI GCGC 2 cut(s) 1815, 2555
Cfr13I GGNCC 5 cut(s) 324, 949, 1670, 1990, 3383
CseI GACGC 3 cut(s) 258, 1907, 2491
CsiI ACCWGGT 1 cut(s) 597
Csp6I GTAC 7 cut(s) 660, 1598, 1757, 2442, 2502, 2662, 3250
CviQI GTAC 7 cut(s) 660, 1598, 1757, 2442, 2502, 2662, 3250
DraI TTTAAA 1 cut(s) 786
DriI GACNNNNNGTC 2 cut(s) 268, 390
Eam1104I CTCTTC 4 cut(s) 189, 1106, 1203, 3222
Eam1105I GACNNNNNGTC 2 cut(s) 268, 390
EarI CTCTTC 4 cut(s) 189, 1106, 1203, 3222
Eco130I CCWWGG 3 cut(s) 2571, 2733, 2790
Eco147I AGGCCT 2 cut(s) 1635, 2466
Eco24I GRGCYC 1 cut(s) 1125
Eco32I GATATC 2 cut(s) 1390, 1460
Eco47I GGWCC 4 cut(s) 324, 1670, 1990, 3383
Eco57I CTGAAG 5 cut(s) 1087, 2818, 3065, 3170, 3549
Eco88I CYCGRG 2 cut(s) 615, 2477
EcoO109I RGGNCCY 1 cut(s) 949
EcoRII CCWGG 6 cut(s) 285, 597, 2266, 2808, 2869, 3358
EcoRV GATATC 2 cut(s) 1390, 1460
EcoT14I CCWWGG 3 cut(s) 2571, 2733, 2790
EcoT22I ATGCAT 5 cut(s) 1414, 1500, 1714, 2606, 2977
EcoT38I GRGCYC 1 cut(s) 1125
ErhI CCWWGG 3 cut(s) 2571, 2733, 2790
Esp3I CGTCTC 2 cut(s) 162, 2474
FalI AAGNNNNNCTT 2 cut(s) 906, 938
FaqI GGGAC 2 cut(s) 2826, 3376
FauI CCCGC 1 cut(s) 598
FauNDI CATATG 1 cut(s) 2130
FbaI TGATCA 2 cut(s) 1336, 2700
FblI GTMKAC 2 cut(s) 360, 2527
Fnu4HI GCNGC 5 cut(s) 477, 982, 2356, 3256, 3544
FokI GGATG 7 cut(s) 209, 291, 352, 640, 659, 1715, 2792
FriOI GRGCYC 1 cut(s) 1125
Fsp4HI GCNGC 5 cut(s) 477, 982, 2356, 3256, 3544
FspBI CTAG 9 cut(s) 57, 482, 870, 953, 1406, 1538, 2063, 2382, 2642
GlaI GCGC 2 cut(s) 1814, 2554
GluI GCNGC 5 cut(s) 477, 982, 2356, 3256, 3544
GsuI CTGGAG 1 cut(s) 2021
HaeIII GGCC 4 cut(s) 951, 1635, 2466, 3358
HapII CCGG 3 cut(s) 185, 276, 2947
HgaI GACGC 3 cut(s) 258, 1907, 2491
HhaI GCGC 2 cut(s) 1815, 2555
Hin1I GRCGYC 1 cut(s) 357
Hin6I GCGC 2 cut(s) 1813, 2553
HinP1I GCGC 2 cut(s) 1813, 2553
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 3 cut(s) 33, 2029, 2111
HpaII CCGG 3 cut(s) 185, 276, 2947
HphI GGTGA 6 cut(s) 109, 562, 1501, 1988, 2642, 3322
Hpy166II GTNNAC 6 cut(s) 361, 1171, 1298, 2077, 2528, 3383
Hpy8I GTNNAC 6 cut(s) 361, 1171, 1298, 2077, 2528, 3383
Hpy99I CGWCG 2 cut(s) 274, 362
HpyAV CCTTC 9 cut(s) 317, 448, 551, 1175, 1625, 1683, 1751, 1900, 2774
HpyCH4III ACNGT 7 cut(s) 1283, 1432, 2501, 2686, 2877, 3157, 3249
HpyCH4IV ACGT 3 cut(s) 357, 1146, 1561
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 482, 870
HpySE526I ACGT 3 cut(s) 357, 1146, 1561
Hsp92I GRCGYC 1 cut(s) 357
HspAI GCGC 2 cut(s) 1813, 2553
Kpn2I TCCGGA 1 cut(s) 275
Ksp22I TGATCA 2 cut(s) 1336, 2700
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 2 cut(s) 379, 1910
Lsp1109I GCAGC 4 cut(s) 968, 2342, 3267, 3555
MabI ACCWGGT 1 cut(s) 597
MaeI CTAG 9 cut(s) 57, 482, 870, 953, 1406, 1538, 2063, 2382, 2642
MaeII ACGT 3 cut(s) 357, 1146, 1561
MfeI CAATTG 2 cut(s) 371, 1626
MflI RGATCY 9 cut(s) 4, 1549, 1935, 2344, 2419, 2542, 2943, 3051, 3268
MhlI GDGCHC 4 cut(s) 519, 1125, 1173, 3038
MluI ACGCGT 1 cut(s) 267
MlyI GAGTC 4 cut(s) 400, 530, 1580, 2627
MmeI TCCRAC 3 cut(s) 583, 2745, 2757
Mph1103I ATGCAT 5 cut(s) 1414, 1500, 1714, 2606, 2977
MroI TCCGGA 1 cut(s) 275
MroXI GAANNNNTTC 7 cut(s) 610, 1185, 1530, 1782, 2742, 2799, 3131
MslI CAYNNNNRTG 5 cut(s) 1652, 2129, 2599, 2747, 2823
MspI CCGG 3 cut(s) 185, 276, 2947
MspR9I CCNGG 8 cut(s) 185, 287, 599, 2268, 2810, 2871, 2948, 3360
MunI CAATTG 2 cut(s) 371, 1626
Mva1269I GAATGC 3 cut(s) 1027, 1437, 3075
MvaI CCWGG 6 cut(s) 287, 599, 2268, 2810, 2871, 3360
MvnI CGCG 3 cut(s) 235, 269, 1815
MwoI GCNNNNNNNGC 3 cut(s) 32, 482, 870
NciI CCSGG 2 cut(s) 185, 2948
NcoI CCATGG 2 cut(s) 2733, 2790
NdeI CATATG 1 cut(s) 2130
NlaIV GGNNCC 7 cut(s) 284, 2317, 2739, 2796, 2868, 3128, 3385
NmuCI GTSAC 6 cut(s) 298, 1511, 1976, 2630, 3151, 3443
NruI TCGCGA 1 cut(s) 235
NsiI ATGCAT 5 cut(s) 1414, 1500, 1714, 2606, 2977
NspI RCATGY 4 cut(s) 1115, 1365, 1416, 1651
PaeI GCATGC 1 cut(s) 1115
PaeR7I CTCGAG 2 cut(s) 615, 2477
PasI CCCWGGG 1 cut(s) 2267
PceI AGGCCT 2 cut(s) 1635, 2466
PciSI GCTCTTC 1 cut(s) 1203
PctI GAATGC 3 cut(s) 1027, 1437, 3075
PdmI GAANNNNTTC 7 cut(s) 610, 1185, 1530, 1782, 2742, 2799, 3131
PflMI CCANNNNNTGG 2 cut(s) 1999, 3086
PfoI TCCNGGA 1 cut(s) 2869
PkrI GCNGC 5 cut(s) 478, 983, 2357, 3257, 3545
PleI GAGTC 4 cut(s) 399, 529, 1580, 2627
PpsI GAGTC 4 cut(s) 399, 529, 1580, 2627
Ppu21I YACGTR 1 cut(s) 1147
PshBI ATTAAT 1 cut(s) 3011
PsiI TTATAA 2 cut(s) 726, 1007
Psp6I CCWGG 6 cut(s) 285, 597, 2266, 2808, 2869, 3358
PspGI CCWGG 6 cut(s) 285, 597, 2266, 2808, 2869, 3358
PspN4I GGNNCC 7 cut(s) 284, 2317, 2739, 2796, 2868, 3128, 3385
PspPI GGNCC 5 cut(s) 324, 949, 1670, 1990, 3383
PsrI GAACNNNNNNTAC 2 cut(s) 2884, 2916
PstNI CAGNNNCTG 2 cut(s) 1999, 2815
PsuI RGATCY 9 cut(s) 4, 1549, 1935, 2344, 2419, 2542, 2943, 3051, 3268
RruI TCGCGA 1 cut(s) 235
RsaI GTAC 7 cut(s) 661, 1599, 1758, 2443, 2503, 2663, 3251
RsaNI GTAC 7 cut(s) 660, 1598, 1757, 2442, 2502, 2662, 3250
RseI CAYNNNNRTG 5 cut(s) 1652, 2129, 2599, 2747, 2823
SalI GTCGAC 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 1203
SatI GCNGC 5 cut(s) 477, 982, 2356, 3256, 3544
Sau96I GGNCC 5 cut(s) 324, 949, 1670, 1990, 3383
ScaI AGTACT 1 cut(s) 661
SchI GAGTC 4 cut(s) 400, 530, 1580, 2627
ScrFI CCNGG 8 cut(s) 185, 287, 599, 2268, 2810, 2871, 2948, 3360
SduI GDGCHC 4 cut(s) 519, 1125, 1173, 3038
SexAI ACCWGGT 1 cut(s) 597
SfcI CTRYAG 2 cut(s) 1233, 1882
Sfr274I CTCGAG 2 cut(s) 615, 2477
SinI GGWCC 4 cut(s) 324, 1670, 1990, 3383
SlaI CTCGAG 2 cut(s) 615, 2477
SmiMI CAYNNNNRTG 5 cut(s) 1652, 2129, 2599, 2747, 2823
SmlI CTYRAG 5 cut(s) 82, 615, 968, 1034, 2477
SmoI CTYRAG 5 cut(s) 82, 615, 968, 1034, 2477
SpeI ACTAGT 1 cut(s) 2641
SphI GCATGC 1 cut(s) 1115
SseBI AGGCCT 2 cut(s) 1635, 2466
SsiI CCGC 3 cut(s) 476, 591, 3378
SspI AATATT 1 cut(s) 3165
SspMI CTAG 9 cut(s) 57, 482, 870, 953, 1406, 1538, 2063, 2382, 2642
StuI AGGCCT 2 cut(s) 1635, 2466
StyD4I CCNGG 8 cut(s) 183, 285, 597, 2266, 2808, 2869, 2946, 3358
StyI CCWWGG 3 cut(s) 2571, 2733, 2790
TaaI ACNGT 7 cut(s) 1283, 1432, 2501, 2686, 2877, 3157, 3249
TaiI ACGT 3 cut(s) 360, 1149, 1564
TaqI TCGA 7 cut(s) 360, 448, 571, 616, 930, 1045, 2478
TatI WGTACW 5 cut(s) 659, 1597, 1756, 2501, 3249
TauI GCSGC 1 cut(s) 479
TscAI CASTG 3 cut(s) 2663, 3160, 3448
TseFI GTSAC 6 cut(s) 298, 1511, 1976, 2630, 3151, 3443
TseI GCWGC 4 cut(s) 981, 2355, 3255, 3543
Tsp45I GTSAC 6 cut(s) 298, 1511, 1976, 2630, 3151, 3443
TspGWI ACGGA 3 cut(s) 1179, 2041, 2871
TspRI CASTG 3 cut(s) 2663, 3160, 3448
Van91I CCANNNNNTGG 2 cut(s) 1999, 3086
VneI GTGCAC 1 cut(s) 1169
VpaK11BI GGWCC 4 cut(s) 324, 1670, 1990, 3383
VspI ATTAAT 1 cut(s) 3011
XapI RAATTY 8 cut(s) 527, 675, 733, 1401, 1681, 1828, 2083, 3551
XbaI TCTAGA 2 cut(s) 1405, 1537
XceI RCATGY 4 cut(s) 1115, 1365, 1416, 1651
XcmI CCANNNNNNNNNTGG 1 cut(s) 3137
XhoI CTCGAG 2 cut(s) 615, 2477
XmiI GTMKAC 2 cut(s) 360, 2527
XmnI GAANNNNTTC 7 cut(s) 610, 1185, 1530, 1782, 2742, 2799, 3131
XspI CTAG 9 cut(s) 57, 482, 870, 953, 1406, 1538, 2063, 2382, 2642
ZraI GACGTC 1 cut(s) 358
ZrmI AGTACT 1 cut(s) 661
Zsp2I ATGCAT 5 cut(s) 1414, 1500, 1714, 2606, 2977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.