MD08G1018200.v1.1

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000036
Physical Location & Seq
Forward (+)
1380760 .. 1388009
7250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1018200.v1.1.491

Sequence Viewer

Length: 3543 bp
ATGGCTCTTGTGAGAACATCTCCAGGAACCTCCTCTGATTCCAACACTTTTCGGAGTTATCGCTACGACGTGTTCTTGAGCTTCAGAGGAGAAACTCGCAAGACTTTTACCGACCACCTCTACACAGCCTTGAACAACGCAGGATTTCTTACGTTCCGAGACGACGACGAACTTGAGAGAGGAGAAGATATAAAGCCAGGACTGCAGAACGCGATCCAGCAGTCACGAACTTCTGTTGTCGTGTTTTCGAAAGATTACGCTTCATCCAGGTGGTGTCTTGATGAGCTTGTGCTGATCCTTGAACGCAAGAGGACCACCTCGGACCATGTTGTTTTACCAGTCTTCTACGATGTCGATCCATCCCACGTGAGGAAGCAGACAGGAAGTGTTGGAAAAGCATTTGCTAGAAGCCAGAAAAATCAGTCGTCGGAAAAGGTGGAGGGATGGAGGAAGGCACTTGCAGAGATTGCAGACCTAGCAGGCATGGTCTTAAAAAATCAAGCTGATGGGTATGAGTCAACGTTTATAAAAAACATTGTTAAAGTGATAGGAGACAAGTTAAGTCGCATGCCATTGAGTGTTGAACCAAAATTGATTGGAATTCAATCTCAAGCCGAACGCATCAATTTGTGGTTACAAGATGGATCAAGTCATGTTGGTATATTTGTTGTGTATGGCATGTCTGGAATAGGGAAGACAACCATTGCAAAACATGTTTACAATTCAAACTTTGGAAGCTTTCAAGGAAGTAGTTTCATTGAAAATATCAAAGAAACAGCAGATCGACCAAATGGCTTAGTTCAAATACAAATGCAACTTCTTTGTGATATTTTGAACGGCAGAGAAGTGAAAATACATAGTGTTAGTGAGGGAATAATTAAGATTGAAAGGGCCATAAGCACTAGAAGAGTTCTACTTGTTCTTGATGATGTTGACCAAATGGACCAATTCGATGCAATCCTAAGGATGAAGGATCAGTTTTATCTTGGAAGTAAGATACTAATAACAACTAGGCGTGAAAGGTTGCTAAAGGCAGATCAAGTTACAGAGGTGCACAGAGTTCGGACTTTGTATTACAATGAATCATTAAAGCTTTTCAGTTGGCATGCTTTTGGCCAGGACCATCCCATAGAAGAATACATGGAGCACTCAAAACAACTAGTGCAACATAGCAGCGGACTTCCCTTAGCTCTAAAAGTTTTGGGTTCTTCTTTATCAGGGGAAAGTATAGCTGTATGGGAAAGTGCATTGGAGAAGCTAAAAGTTATTCCTGATGGTGAAATCATGAGTAAACTAAGAATAAGCTATGAAAGTTTAAAAGATGACCATGACCAGAAATTATTCCTCCACATTGCTTGTTTCCTAATAGGAAGGAACAAAAGCTACATTGTTAGAATACTCGATGGATGTGATTTCTATACAATCATTGGCATTCAAAATCTCATTGATAGATGCTTGGTGACACTTGATAAATACGGCAAGGTGGAAATGCATGACATGATTCGTGCTATGGGAAGAGAAATTGTTCGCCAAGAATCAGAAGAGCCTGAGGAACGGAGTAGATTATGGCGCCATAAGGATTCTTTCCAAGTATTGAGGAAAAAGAATGGTACAAAAAAAATCCAAGGTCTTGTCCTGGATATGCGTAATCATCCTGCAAACAGTCCAATAAACACAAATGAGATAGTCTTGGAAACCAATGCATTTGCAAGGATGCGTAATTTACAACTACTCCATCTTAGTCATGTACGACTGGATGGATGTTATGCACTTTTCCCTACAGGATTAAGATGGTTGTGTTGGCTTGAATTTCCTTTGGATTCTATACCAATTGATTTTCCTTTGGAGAACGTAATTGTTCTTGAAATGCAATACAGTAGCTTGAGACAAGTATGCAAAGGAACAAAATTTCTTCCATCGTTGAAGATCCTTGACGTCAGCCATTCTCATGGCCTTAGTGAAACCATTGACTTCTCACTTTTCCCCAATCTAGAGGAACTGATTCTTGTAGGTTGCACAAGCCTGAAGAATGTTCATGAATCCATTGGAAACTTGGAGAGACTCGTGTACTTGAATATGAAGGATTGCAAGAATCTTAGGAAGCTTCCAAAGAACATGTGCATGCTTAAATCACTTGAAACACTCATTTTATCTGATTGCTCAAATCTTGTTGAGTTTCCAGTAGAGATGATGAAGAACATGGAGTCTCTGAAAGTTCTTGAGACAGATGGAATTCTAATAGGTGAATTGAGGCCAGAAAGAAGTTCAACCATCTTGAGTTCTTTTCCATGCTCTTTAGTAGAGTTAAGTATGAAGAGGTGCAATCTTTCTGATGATGTCCTTCCTACGGATTTAAGTAATCTATCCTACTTGCGAAGACTACATTTAGATGAGAATCCAATTTGCAGTCTGCCAGTTTTCATCAAAGGTTTGAGGAGGATCGATGAACTCTCTTTCGAGGGTTGTGATAGGCTCGAATCGCTTGTGGGGTTGCCGGAATTACACCAAGGGCTAAGTATAGTCGGATGCATATCATTAAAAAAAATAACTTTTCATTCCCCTGAGCGGTTGTGTCCAATTTTTTTTTGTGACAACTGGAATCTTGTTGAGTGGGAGCGCTGTTACAAGTTAGAGCCTATTGATAGAGTTGATGTGGAAATGATCAAACTTTTGGGCTTGTGCAACTTGGAATCCATGCCAGCTGTTCGAATGAATTATCTACACTCATACAAGGATCGAAAAGAGGTCCCCGTCCAGGAACTGTGTCAACGTGGTATATTCAGAACGTTTTTTGTTGGGAATGAGGTTCCAGGCCAGTTCAGCTATATAAGTACCAAGTCCTCGATATCTTTTATTGTCCCTTTACTACATGCTAGTCACAGAATTCGAGGCTTGAACATCTTTGCTACCTATGCAAAGAAAAATTCAAATAATTATTCTACTATACCTATTATTGGTCCAATAATGATCAAAGTGAGAAACAAGAGTAAGGGTCTGAAGTGGATCTATAGCCCAGAGTTCCGTGGAATTCCAGGTGACGGAGAAGATATGATATGGTTGAGCCATTGTAAGATGGAGAATAAAACAATATTACAATGTGGAGATGAAGTGGTGGTTTCGGTAATATCCGGACGACGTGACTTGTTTTGGGTAAAGGAGTTTGGTGTCCAGCTTGTGCAAGAGCACCAAGACAGGATGAGTACCCAACACAACAAATCAGATCCTAATTATGCATTTGTTATCGGTGGAGATTTGAAGATGTGGGAGTGCAGTTTGGGATTATACCTCATTGGCGGTTATGAAGGAGGAGATATTGACCGCATTGATATCCTCACCATGGACTCTGATGAAGAAGACACAAACAAAGAAGGGCAAGAGGATGAACCTGATTACACAATTGCAAAGACGAGGGCTGCTAGCAATAACTGCAGCCTGAGAGGCTGGAAGGTGCGCCTCACAGCTGTCGGCTTCTTTTTCGCGCTGGCTCTAGTAGGTTGGCCCTCCCAAAAAAAGAAGCGACACTCGTCCACAAGCCCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1181

Amino Acids

134.53

Weight (kDa)

7.02

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 193 2.9e-50 TIR domain
TIR_2 PF13676 24 - 120 1.3e-15 TIR domain
NB-ARC PF00931 203 - 372 5.3e-20 NB-ARC domain
WHD_ROQ1 PF23282 442 - 513 1.5e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 656 - 743 3.2e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
AatII GACGTC 1 cut(s) 1938
AccB1I GGYRCC 1 cut(s) 1569
AccBSI CCGCTC 1 cut(s) 2566
AccII CGCG 2 cut(s) 212, 3479
AccIII TCCGGA 1 cut(s) 3128
AciI CCGC 4 cut(s) 1176, 2566, 3294, 3319
AclI AACGTT 2 cut(s) 521, 2786
AcoI YGGCCR 1 cut(s) 1114
AcsI RAATTY 7 cut(s) 600, 1808, 1907, 2232, 2883, 2923, 3027
AcuI CTGAAG 3 cut(s) 67, 2045, 3017
AcvI CACGTG 1 cut(s) 367
AcyI GRCGYC 2 cut(s) 1570, 1935
AfaI GTAC 5 cut(s) 1612, 1749, 2069, 2833, 3202
AfeI AGCGCT 1 cut(s) 2618
AfiI CCNNNNNNNGG 7 cut(s) 369, 2347, 2565, 2755, 2954, 3038, 3337
AflIII ACRYGT 3 cut(s) 69, 712, 2115
AhlI ACTAGT 1 cut(s) 1159
AjiI CACGTC 2 cut(s) 70, 3137
AjnI CCWGG 8 cut(s) 22, 196, 266, 1116, 1635, 2754, 2809, 3031
Alw21I GWGCWC 3 cut(s) 1056, 1149, 3186
Alw26I GTCTC 6 cut(s) 153, 546, 1879, 2053, 2211, 2216
Alw44I GTGCAC 1 cut(s) 1052
AlwNI CAGNNNCTG 1 cut(s) 2761
Aor13HI TCCGGA 1 cut(s) 3128
Aor51HI AGCGCT 1 cut(s) 2618
AoxI GGCC 6 cut(s) 891, 1114, 1951, 2252, 2812, 3497
ApaLI GTGCAC 1 cut(s) 1052
ApeKI GCWGC 3 cut(s) 1173, 3413, 3429
ApoI RAATTY 7 cut(s) 600, 1808, 1907, 2232, 2883, 2923, 3027
Asp700I GAANNNNTTC 3 cut(s) 1340, 1524, 2001
AspLEI GCGC 4 cut(s) 1572, 2619, 3453, 3481
AspS9I GGNCC 8 cut(s) 312, 322, 891, 943, 1120, 2746, 2957, 3498
AsuHPI GGTGA 5 cut(s) 1289, 1471, 2255, 3047, 3325
AsuII TTCGAA 2 cut(s) 248, 2707
AsuNHI GCTAGC 1 cut(s) 3416
AvaII GGWCC 6 cut(s) 312, 322, 943, 1120, 2746, 2957
AxyI CCTNAGG 2 cut(s) 962, 1548
BaeGI GKGCMC 1 cut(s) 1056
BalI TGGCCA 1 cut(s) 1116
BanI GGYRCC 1 cut(s) 1569
BarI GAAGNNNNNNTAC 2 cut(s) 837, 869
BauI CACGAG 1 cut(s) 2063
BbrPI CACGTG 1 cut(s) 367
BbsI GAAGAC 4 cut(s) 334, 701, 2383, 3360
Bbv12I GWGCWC 3 cut(s) 1056, 1149, 3186
BbvI GCAGC 3 cut(s) 1185, 3400, 3441
BceAI ACGGC 2 cut(s) 853, 1492
BcgI CGANNNNNNTGC 2 cut(s) 1043, 1077
BciT130I CCWGG 8 cut(s) 24, 198, 268, 1118, 1637, 2756, 2811, 3033
BclI TGATCA 2 cut(s) 2661, 2967
BcoDI GTCTC 6 cut(s) 153, 546, 1879, 2053, 2211, 2216
BcuI ACTAGT 1 cut(s) 1159
BfaI CTAG 9 cut(s) 405, 476, 903, 1011, 1160, 1991, 2874, 3417, 3488
BfmI CTRYAG 4 cut(s) 203, 1779, 3007, 3427
BfoI RGCGCY 2 cut(s) 1573, 2620
BglI GCCNNNNNGGC 1 cut(s) 3438
BisI GCNGC 3 cut(s) 1174, 3414, 3430
BlsI GCNGC 3 cut(s) 1175, 3415, 3431
Bme1390I CCNGG 8 cut(s) 24, 198, 268, 1118, 1637, 2756, 2811, 3033
Bme18I GGWCC 6 cut(s) 312, 322, 943, 1120, 2746, 2957
BmgBI CACGTC 2 cut(s) 70, 3137
BmgT120I GGNCC 8 cut(s) 312, 322, 891, 943, 1120, 2746, 2957, 3498
BmiI GGNNCC 4 cut(s) 28, 1571, 2748, 2808
BmrFI CCNGG 8 cut(s) 24, 198, 268, 1118, 1637, 2756, 2811, 3033
BmsI GCATC 5 cut(s) 630, 943, 1442, 1704, 2516
BmtI GCTAGC 1 cut(s) 3420
BoxI GACNNNNGTC 1 cut(s) 3523
BpiI GAAGAC 4 cut(s) 334, 701, 2383, 3360
BplI GAGNNNNNCTC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 6
Bpu10I CCTNAGC 2 cut(s) 1186, 2562
Bpu14I TTCGAA 2 cut(s) 248, 2707
BpuEI CTTGAG 6 cut(s) 97, 194, 594, 1903, 2240, 2296
Bsa29I ATCGAT 1 cut(s) 2442
BsaAI YACGTR 1 cut(s) 367
BsaBI GATNNNNATC 3 cut(s) 354, 2394, 2530
BsaHI GRCGYC 2 cut(s) 1570, 1935
BsaJI CCNNGG 5 cut(s) 318, 1624, 2506, 3022, 3336
BsaWI WCCGGW 1 cut(s) 3128
BsaXI ACNNNNNCTCC 4 cut(s) 1716, 1746, 3093, 3123
Bsc4I CCNNNNNNNGG 7 cut(s) 369, 2347, 2565, 2755, 2954, 3038, 3337
Bse1I ACTGG 6 cut(s) 338, 1758, 2180, 2414, 2600, 2815
Bse21I CCTNAGG 2 cut(s) 962, 1548
Bse3DI GCAATG 2 cut(s) 702, 1350
Bse8I GATNNNNATC 3 cut(s) 354, 2394, 2530
BseAI TCCGGA 1 cut(s) 3128
BseBI CCWGG 8 cut(s) 24, 198, 268, 1118, 1637, 2756, 2811, 3033
BseCI ATCGAT 1 cut(s) 2442
BseDI CCNNGG 5 cut(s) 318, 1624, 2506, 3022, 3336
BseJI GATNNNNATC 3 cut(s) 354, 2394, 2530
BseLI CCNNNNNNNGG 7 cut(s) 369, 2347, 2565, 2755, 2954, 3038, 3337
BseMI GCAATG 2 cut(s) 702, 1350
BseMII CTCAG 3 cut(s) 1539, 2553, 3425
BseNI ACTGG 6 cut(s) 338, 1758, 2180, 2414, 2600, 2815
BseRI GAGGAG 5 cut(s) 22, 102, 195, 2449, 3321
BseSI GKGCMC 1 cut(s) 1056
BseXI GCAGC 3 cut(s) 1185, 3400, 3441
BsgI GTGCAG 1 cut(s) 3289
Bsh1236I CGCG 2 cut(s) 212, 3479
BshFI GGCC 6 cut(s) 893, 1116, 1953, 2254, 2814, 3499
BshNI GGYRCC 1 cut(s) 1569
BshVI ATCGAT 1 cut(s) 2442
BsiHKAI GWGCWC 3 cut(s) 1056, 1149, 3186
BsiSI CCGG 2 cut(s) 2495, 3129
BslFI GGGAC 2 cut(s) 2732, 2843
BslI CCNNNNNNNGG 7 cut(s) 369, 2347, 2565, 2755, 2954, 3038, 3337
BsmAI GTCTC 6 cut(s) 153, 546, 1879, 2053, 2211, 2216
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 2 cut(s) 2732, 2843
BsmI GAATGC 1 cut(s) 1431
BsnI GGCC 6 cut(s) 893, 1116, 1953, 2254, 2814, 3499
Bsp119I TTCGAA 2 cut(s) 248, 2707
Bsp1286I GDGCHC 3 cut(s) 1056, 1149, 3186
Bsp13I TCCGGA 1 cut(s) 3128
Bsp19I CCATGG 1 cut(s) 3336
BspACI CCGC 4 cut(s) 1176, 2566, 3294, 3319
BspANI GGCC 6 cut(s) 893, 1116, 1953, 2254, 2814, 3499
BspCNI CTCAG 3 cut(s) 1540, 2554, 3426
BspDI ATCGAT 1 cut(s) 2442
BspEI TCCGGA 1 cut(s) 3128
BspFNI CGCG 2 cut(s) 212, 3479
BspHI TCATGA 2 cut(s) 1284, 2035
BspLI GGNNCC 4 cut(s) 28, 1571, 2748, 2808
BspMAI CTGCAG 2 cut(s) 207, 3431
BspOI GCTAGC 1 cut(s) 3420
BspQI GCTCTTC 1 cut(s) 1536
BspT104I TTCGAA 2 cut(s) 248, 2707
BspT107I GGYRCC 1 cut(s) 1569
BsrBI CCGCTC 1 cut(s) 2566
BsrDI GCAATG 2 cut(s) 702, 1350
BsrI ACTGG 6 cut(s) 338, 1758, 2180, 2414, 2600, 2815
BssECI CCNNGG 5 cut(s) 318, 1624, 2506, 3022, 3336
BssNI GRCGYC 2 cut(s) 1570, 1935
BssSI CACGAG 1 cut(s) 2063
BssT1I CCWWGG 3 cut(s) 1624, 2506, 3336
Bst2BI CACGAG 1 cut(s) 2063
Bst2UI CCWGG 8 cut(s) 24, 198, 268, 1118, 1637, 2756, 2811, 3033
Bst4CI ACNGT 3 cut(s) 1664, 1877, 2763
Bst6I CTCTTC 4 cut(s) 901, 1510, 1536, 2309
BstACI GRCGYC 2 cut(s) 1570, 1935
BstAPI GCANNNNNTGC 2 cut(s) 467, 3426
BstBAI YACGTR 1 cut(s) 367
BstBI TTCGAA 2 cut(s) 248, 2707
BstC8I GCNNGC 7 cut(s) 481, 569, 1107, 2123, 2700, 3418, 3483
BstDSI CCRYGG 2 cut(s) 3022, 3336
BstFNI CGCG 2 cut(s) 212, 3479
BstH2I RGCGCY 2 cut(s) 1573, 2620
BstHHI GCGC 4 cut(s) 1572, 2619, 3453, 3481
BstMAI GTCTC 6 cut(s) 153, 546, 1879, 2053, 2211, 2216
BstMWI GCNNNNNNNGC 8 cut(s) 202, 467, 476, 2479, 2820, 2912, 3426, 3438
BstNI CCWGG 8 cut(s) 24, 198, 268, 1118, 1637, 2756, 2811, 3033
BstNSI RCATGY 7 cut(s) 571, 682, 716, 1109, 2119, 2125, 2873
BstPAI GACNNNNGTC 1 cut(s) 3523
BstSCI CCNGG 8 cut(s) 22, 196, 266, 1116, 1635, 2754, 2809, 3031
BstSFI CTRYAG 4 cut(s) 203, 1779, 3007, 3427
BstSLI GKGCMC 1 cut(s) 1056
BstUI CGCG 2 cut(s) 212, 3479
BstV1I GCAGC 3 cut(s) 1185, 3400, 3441
BstV2I GAAGAC 4 cut(s) 334, 701, 2383, 3360
BstX2I RGATCY 3 cut(s) 1926, 3003, 3220
BstXI CCANNNNNNTGG 1 cut(s) 1949
BstYI RGATCY 3 cut(s) 1926, 3003, 3220
Bsu15I ATCGAT 1 cut(s) 2442
Bsu36I CCTNAGG 2 cut(s) 962, 1548
BsuRI GGCC 6 cut(s) 893, 1116, 1953, 2254, 2814, 3499
BsuTUI ATCGAT 1 cut(s) 2442
BtgI CCRYGG 2 cut(s) 3022, 3336
BtrI CACGTC 2 cut(s) 70, 3137
Cac8I GCNNGC 7 cut(s) 481, 569, 1107, 2123, 2700, 3418, 3483
CaiI CAGNNNCTG 1 cut(s) 2761
CciI TCATGA 2 cut(s) 1284, 2035
CfoI GCGC 4 cut(s) 1572, 2619, 3453, 3481
Cfr13I GGNCC 8 cut(s) 312, 322, 891, 943, 1120, 2746, 2957, 3498
ClaI ATCGAT 1 cut(s) 2442
Csp6I GTAC 5 cut(s) 1611, 1748, 2068, 2832, 3201
CspCI CAANNNNNGTGG 2 cut(s) 1337, 1372
CviQI GTAC 5 cut(s) 1611, 1748, 2068, 2832, 3201
DinI GGCGCC 1 cut(s) 1571
DraI TTTAAA 1 cut(s) 1317
EaeI YGGCCR 1 cut(s) 1114
Eam1104I CTCTTC 4 cut(s) 901, 1510, 1536, 2309
EarI CTCTTC 4 cut(s) 901, 1510, 1536, 2309
Eco130I CCWWGG 3 cut(s) 1624, 2506, 3336
Eco32I GATATC 2 cut(s) 2847, 3328
Eco47I GGWCC 6 cut(s) 312, 322, 943, 1120, 2746, 2957
Eco47III AGCGCT 1 cut(s) 2618
Eco57I CTGAAG 3 cut(s) 67, 2045, 3017
Eco72I CACGTG 1 cut(s) 367
Eco81I CCTNAGG 2 cut(s) 962, 1548
EcoO109I RGGNCCY 1 cut(s) 2746
EcoRI GAATTC 4 cut(s) 600, 2232, 2883, 3027
EcoRII CCWGG 8 cut(s) 22, 196, 266, 1116, 1635, 2754, 2809, 3031
EcoRV GATATC 2 cut(s) 2847, 3328
EcoT14I CCWWGG 3 cut(s) 1624, 2506, 3336
EcoT22I ATGCAT 4 cut(s) 1494, 1705, 2531, 3235
EgeI GGCGCC 1 cut(s) 1571
EheI GGCGCC 1 cut(s) 1571
ErhI CCWWGG 3 cut(s) 1624, 2506, 3336
Esp3I CGTCTC 1 cut(s) 153
FaqI GGGAC 2 cut(s) 2732, 2843
FbaI TGATCA 2 cut(s) 2661, 2967
Fnu4HI GCNGC 3 cut(s) 1174, 3414, 3430
Fsp4HI GCNGC 3 cut(s) 1174, 3414, 3430
FspBI CTAG 9 cut(s) 405, 476, 903, 1011, 1160, 1991, 2874, 3417, 3488
GlaI GCGC 4 cut(s) 1571, 2618, 3452, 3480
GluI GCNGC 3 cut(s) 1174, 3414, 3430
GsuI CTGGAG 1 cut(s) 6
HaeII RGCGCY 2 cut(s) 1573, 2620
HaeIII GGCC 6 cut(s) 893, 1116, 1953, 2254, 2814, 3499
HapII CCGG 2 cut(s) 2495, 3129
HhaI GCGC 4 cut(s) 1572, 2619, 3453, 3481
Hin1I GRCGYC 2 cut(s) 1570, 1935
Hin6I GCGC 4 cut(s) 1570, 2617, 3451, 3479
HinP1I GCGC 4 cut(s) 1570, 2617, 3451, 3479
HincII GTYRAC 3 cut(s) 519, 934, 2768
HindII GTYRAC 3 cut(s) 519, 934, 2768
HindIII AAGCTT 3 cut(s) 736, 1091, 2102
HpaII CCGG 2 cut(s) 2495, 3129
HphI GGTGA 5 cut(s) 1289, 1471, 2255, 3047, 3325
Hpy166II GTNNAC 8 cut(s) 519, 718, 934, 1054, 1292, 2068, 2768, 3528
Hpy8I GTNNAC 8 cut(s) 519, 718, 934, 1054, 1292, 2068, 2768, 3528
Hpy99I CGWCG 5 cut(s) 71, 167, 170, 430, 3138
HpyAV CCTTC 8 cut(s) 445, 964, 1365, 2074, 2351, 3296, 3362, 3439
HpyCH4III ACNGT 3 cut(s) 1664, 1877, 2763
HpyCH4IV ACGT 9 cut(s) 69, 152, 366, 521, 1851, 1935, 2770, 2786, 3136
HpyF10VI GCNNNNNNNGC 8 cut(s) 202, 467, 476, 2479, 2820, 2912, 3426, 3438
HpySE526I ACGT 9 cut(s) 69, 152, 366, 521, 1851, 1935, 2770, 2786, 3136
Hsp92I GRCGYC 2 cut(s) 1570, 1935
HspAI GCGC 4 cut(s) 1570, 2617, 3451, 3479
KasI GGCGCC 1 cut(s) 1569
Kpn2I TCCGGA 1 cut(s) 3128
Ksp22I TGATCA 2 cut(s) 2661, 2967
LguI GCTCTTC 1 cut(s) 1536
LmnI GCTCC 2 cut(s) 1144, 2614
Lsp1109I GCAGC 3 cut(s) 1185, 3400, 3441
LweI GCATC 5 cut(s) 630, 943, 1442, 1704, 2516
MaeI CTAG 9 cut(s) 405, 476, 903, 1011, 1160, 1991, 2874, 3417, 3488
MaeII ACGT 9 cut(s) 69, 152, 366, 521, 1851, 1935, 2770, 2786, 3136
MaeIII GTNAC 9 cut(s) 222, 633, 1042, 1459, 2588, 2621, 2876, 3035, 3137
MbiI CCGCTC 1 cut(s) 2566
MfeI CAATTG 2 cut(s) 1830, 3396
MflI RGATCY 3 cut(s) 1926, 3003, 3220
MhlI GDGCHC 3 cut(s) 1056, 1149, 3186
MlsI TGGCCA 1 cut(s) 1116
MluNI TGGCCA 1 cut(s) 1116
Mly113I GGCGCC 1 cut(s) 1570
MlyI GAGTC 4 cut(s) 524, 2055, 2213, 3335
MmeI TCCRAC 4 cut(s) 66, 370, 408, 2503
Mox20I TGGCCA 1 cut(s) 1116
Mph1103I ATGCAT 4 cut(s) 1494, 1705, 2531, 3235
MroI TCCGGA 1 cut(s) 3128
MroXI GAANNNNTTC 3 cut(s) 1340, 1524, 2001
MscI TGGCCA 1 cut(s) 1116
MslI CAYNNNNRTG 4 cut(s) 268, 1947, 2120, 2388
Msp20I TGGCCA 1 cut(s) 1116
MspA1I CMGCKG 3 cut(s) 1176, 2702, 3461
MspI CCGG 2 cut(s) 2495, 3129
MspR9I CCNGG 8 cut(s) 24, 198, 268, 1118, 1637, 2756, 2811, 3033
MunI CAATTG 2 cut(s) 1830, 3396
Mva1269I GAATGC 1 cut(s) 1431
MvaI CCWGG 8 cut(s) 24, 198, 268, 1118, 1637, 2756, 2811, 3033
MvnI CGCG 2 cut(s) 212, 3479
MwoI GCNNNNNNNGC 8 cut(s) 202, 467, 476, 2479, 2820, 2912, 3426, 3438
NarI GGCGCC 1 cut(s) 1570
NcoI CCATGG 1 cut(s) 3336
NheI GCTAGC 1 cut(s) 3416
NlaIV GGNNCC 4 cut(s) 28, 1571, 2748, 2808
NmuCI GTSAC 6 cut(s) 222, 1459, 2588, 2876, 3035, 3137
NsiI ATGCAT 4 cut(s) 1494, 1705, 2531, 3235
NspI RCATGY 7 cut(s) 571, 682, 716, 1109, 2119, 2125, 2873
NspV TTCGAA 2 cut(s) 248, 2707
PaeI GCATGC 3 cut(s) 571, 1109, 2125
PagI TCATGA 2 cut(s) 1284, 2035
PciI ACATGT 2 cut(s) 712, 2115
PciSI GCTCTTC 1 cut(s) 1536
PctI GAATGC 1 cut(s) 1431
PdmI GAANNNNTTC 3 cut(s) 1340, 1524, 2001
PfoI TCCNGGA 3 cut(s) 22, 1635, 2754
PkrI GCNGC 3 cut(s) 1175, 3415, 3431
PleI GAGTC 4 cut(s) 523, 2055, 2212, 3335
PluTI GGCGCC 1 cut(s) 1573
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
PpsI GAGTC 4 cut(s) 523, 2055, 2212, 3335
Ppu21I YACGTR 1 cut(s) 367
PpuMI RGGWCCY 1 cut(s) 2746
PscI ACATGT 2 cut(s) 712, 2115
PshAI GACNNNNGTC 1 cut(s) 3523
PsiI TTATAA 1 cut(s) 527
Psp1406I AACGTT 2 cut(s) 521, 2786
Psp5II RGGWCCY 1 cut(s) 2746
Psp6I CCWGG 8 cut(s) 22, 196, 266, 1116, 1635, 2754, 2809, 3031
PspCI CACGTG 1 cut(s) 367
PspGI CCWGG 8 cut(s) 22, 196, 266, 1116, 1635, 2754, 2809, 3031
PspN4I GGNNCC 4 cut(s) 28, 1571, 2748, 2808
PspPI GGNCC 8 cut(s) 312, 322, 891, 943, 1120, 2746, 2957, 3498
PspPPI RGGWCCY 1 cut(s) 2746
PsrI GAACNNNNNNTAC 2 cut(s) 1367, 1399
PstI CTGCAG 2 cut(s) 207, 3431
PstNI CAGNNNCTG 1 cut(s) 2761
PsuI RGATCY 3 cut(s) 1926, 3003, 3220
PvuII CAGCTG 2 cut(s) 2702, 3461
RsaI GTAC 5 cut(s) 1612, 1749, 2069, 2833, 3202
RsaNI GTAC 5 cut(s) 1611, 1748, 2068, 2832, 3201
RseI CAYNNNNRTG 4 cut(s) 268, 1947, 2120, 2388
SapI GCTCTTC 1 cut(s) 1536
SatI GCNGC 3 cut(s) 1174, 3414, 3430
Sau96I GGNCC 8 cut(s) 312, 322, 891, 943, 1120, 2746, 2957, 3498
SchI GAGTC 4 cut(s) 524, 2055, 2213, 3335
ScrFI CCNGG 8 cut(s) 24, 198, 268, 1118, 1637, 2756, 2811, 3033
SduI GDGCHC 3 cut(s) 1056, 1149, 3186
SfaNI GCATC 5 cut(s) 630, 943, 1442, 1704, 2516
SfcI CTRYAG 4 cut(s) 203, 1779, 3007, 3427
SfoI GGCGCC 1 cut(s) 1571
SfuI TTCGAA 2 cut(s) 248, 2707
SinI GGWCC 6 cut(s) 312, 322, 943, 1120, 2746, 2957
SmiMI CAYNNNNRTG 4 cut(s) 268, 1947, 2120, 2388
SmlI CTYRAG 6 cut(s) 76, 173, 609, 1882, 2219, 2275
SmoI CTYRAG 6 cut(s) 76, 173, 609, 1882, 2219, 2275
SpeI ACTAGT 1 cut(s) 1159
SphI GCATGC 3 cut(s) 571, 1109, 2125
SsiI CCGC 4 cut(s) 1176, 2566, 3294, 3319
SspDI GGCGCC 1 cut(s) 1569
SspI AATATT 1 cut(s) 3090
SspMI CTAG 9 cut(s) 405, 476, 903, 1011, 1160, 1991, 2874, 3417, 3488
StyD4I CCNGG 8 cut(s) 22, 196, 266, 1116, 1635, 2754, 2809, 3031
StyI CCWWGG 3 cut(s) 1624, 2506, 3336
TaaI ACNGT 3 cut(s) 1664, 1877, 2763
TaiI ACGT 9 cut(s) 72, 155, 369, 524, 1854, 1938, 2773, 2789, 3139
TatI WGTACW 1 cut(s) 2067
TseFI GTSAC 6 cut(s) 222, 1459, 2588, 2876, 3035, 3137
TseI GCWGC 3 cut(s) 1173, 3413, 3429
Tsp45I GTSAC 6 cut(s) 222, 1459, 2588, 2876, 3035, 3137
TspGWI ACGGA 4 cut(s) 1570, 2363, 3011, 3054
VneI GTGCAC 1 cut(s) 1052
VpaK11BI GGWCC 6 cut(s) 312, 322, 943, 1120, 2746, 2957
XapI RAATTY 7 cut(s) 600, 1808, 1907, 2232, 2883, 2923, 3027
XbaI TCTAGA 1 cut(s) 1990
XceI RCATGY 7 cut(s) 571, 682, 716, 1109, 2119, 2125, 2873
XcmI CCANNNNNNNNNTGG 1 cut(s) 2050
XmnI GAANNNNTTC 3 cut(s) 1340, 1524, 2001
XspI CTAG 9 cut(s) 405, 476, 903, 1011, 1160, 1991, 2874, 3417, 3488
ZraI GACGTC 1 cut(s) 1936
Zsp2I ATGCAT 4 cut(s) 1494, 1705, 2531, 3235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.