MD05G1152200.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000036
Physical Location & Seq
Forward (+)
28280382 .. 28287965
7584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1152200.v1.1.491

Sequence Viewer

Length: 3582 bp
ATGGCTCAACAAGCTTCCTCATCTCATAGCACTTCTAGTTCTTCTTGGTGCAGTTATGATGTGTTCTTGAGTTTCAGAGGCGAGGACACTCGCAAGACTTTTACTGACCACCTCTATACAGCCTTTGTTAACGCAAACTTTCGAACGTTCCGAGATAATGATGAACTTGAGAGAGGGGAAGATATTAAACCAGGATTCAAGAGAGCCATCCAACAGTCACGGAGTTCTGTCGTTGTTTTTTCTAAAGACTATGCGTCTTCCAAATGGTGCCTTAACGAGCTTGTGATGATCCTCAAACTCAAGAAGACCTCCGACCATGTAGTTTTACCAGTCTTCTACGATGTTGATCCGTCTCATTTGAGGAAGCAAACAGGAAGCCTTGCAGAAGCATTTGCTAAACATCAAAAGGTTCAATCGTTTGACACGGTGAAGGAATGGAGGGAAGCACTTGCTGAAGCTGCAGATCTGGCAGGAATGGTCTTACAGAATGAAGAAGATGGGCACGAGGCAAAGTTTATCCAGAAAATTGTTAAAGTGATTGAAGACAAACTAAGGCGGACGCACGTAAGTTTTGATCCGTACCTGATTGGAATCCATTCTCGAGTACAAAGCATCAACTCATGGTTACAAGATCGATCAAAAGAGGTTGGCATACTTGTCATTTATGGAATGCATGGAATGGGAAAGACAACCATGGCAAAATTTGTTTACAAATCAAATTTTGAAATATTTGAAAGACACAGTTTCCTTGAAAATATCAGAGAAACTGCAGAAAAACCAAACGGCTCGATTCAAATACAAAAGCAACTTCTTCATGATATTACGAATGGGAGAAAAATAAAAATATACAGCACAAGCCAGGGAATGTCTGCAATTGAAGATGTCATTAGCTCTAAAAGAGTTCTTCTCGTTCTAGATGATGTTGACGATATGGATCAACTATTAGGTGAGGTAATTGGGATGCAAGATCGGTTATATCCAGGAAGTAAGATCATTGTAACAACTACCTATGCTGGGTTGTTAAATGCTCACCAACAAGTCTTGTCTCACAATGCTGAAACTTTGAATGACGATGAATCATTGGAGCTCTTCAGTTTACATGCTTTTGGGCAGGACATACCCACTGAAAGATACATGGATCATTCAAGAAGGGTGGTACAACATTGTGGAGGACTTCCACTGGCTCTTAAAGTTTTGGGTTCTTCTCTATCAGGAAAGAGGGTAGCTATATGGGAAAGTACACTGAACAAACTGGAAACTATTCCAAATGGTCAAATCTCGCAAAAACTCAAACTAAGCTACGACTCCTTACAAGACACCCATGATCAAGATTTGTTCCTCCATATTGCATGCTTCTTTATTGGAATGGACAGAGATGACATCGTCAGAATTCTAGATGAGTGTGATTTCTTCACAGATGTTGGCATCCAAAATCTAATGGATAGATATTTAGTCACTCTTAATGGATATAATCAGGTGCAGATGCATCACATGATCCGCGACATGGGAAGAGGAATTGTACGCCTTGAATCAAAGGAACCTGGGGAACGTAGTAGATTATGGCATCATAAGGATTCTTTCAATGTATTGACAGAAAAGAATGGTACGAAAAAAATTGAAGGTCTAATTCTCAACATGCAAATGCACCCAGCATACGCTCGTTTGAGAAACTCAAATGATCAGACAGTTTTGGAGACCAATGGGTTTGCACAGATGCACAAATTAAAACTCCTCCAGCTTAGTTACGTACAACTCAATGGATGCTACAAAGAATTTTCCACAGGATTAAGATGGTTATGTTGGTTTAAATTTCCCTTTGATTCTTTGCCCAGTAATTTTCCATTGGAGAGCCTTGTTGCTCTTGAAATGTGCTGTAGCAGCTTGAGACAAGTTTGGAAGGGAACTGAGCTTCTCCCATCACTAAAGATCCTAAATCTTAGCCATTCCCGTGACCTTACAAAAACGCCCGACTTTTCGTTCGTCCCCAAGCTAGAACGGCTCATTCTCAAAAATTGTGAAAGTTTGATTCATGTTGACGAATCCATTGGAAACCTAGAGGGACTTGTTCATTTAAATATGGAAGATTGCAAAAGTATTACGAAGCTCCCGCGTAATATTTCTATGCTAACCTCCCTTGAAACACTTATTATATCTGGTTGCTCAAATCTCAGCATGTTTTCCCTGGACATGAGGGAGATGGAATCTCTGAAAGTGTTTGAAGCTGATGGTGTTCCGGTACATCGATTACTCACTGCCGCCGAGGAGGTCACATTATGTCCAAGACCAAATGTAGAAATTTCTCGGGCTTCTTATCTTCCACGCAATTTAGTAGACTTAAGACTTAGTAACTGCAATCTTTCAAATGATGATTTTCCAAGAGAGCTTGGTAGTCTATCTTCGTTGAAAATTTTAGATCTGGGTAGTAATCCAATTTGTAGCCTACCGGATTGTGTCAGAGGTATTACAGGGCTTGATAGTCTCTCTTTTTATAATTGTACGAGGCTCAAAAGTCTTGTAAGGCTACCAACTGTAGGAAATTTGTCAATCCTTGGTTGCAAAGCATTGGGAAAGGTGACATTTCAATCATTCAGTCAGGTGAACTCCAGAATGAGGTCTGTCAGATACACGGGAATCCGCAATGTAGATGAGGTATTGGAGTATGAGTACCGGTACAAGATAGAGCCGATTGAAATTGTTGATATGGAAATGATCAACCTGTTGGGTTTGGGAAACTTAAAATATTTCACAGAATCCTTTGTAATGTCCCAGTATTTACGCCTGGCCTTTGGTTGGAAGGCGGTTCGTCGTCCCATCCAGGGACTGCAAGAATGTGGCATATTCAGCACGTTTCTTCCGGGTAATGAGGTTCCTCAATATGGCTTGTTCAGCCATAAAAGTAATGGGTCCTTAATATCTTTTACTGTGCCTGTACTTCCCAATCGCAGGTTCCGAGGCTTGAACATCTTCTCTGTCTATGCAAAATCCATCAGCAATGATTCTCCTAACATTAGTGCCAGTTTAGAGAGTTTACATTGTGCAATATTTACTAAAGTTAGTAATAAGAGTAAGGGTTTGGAGTGGATCTATGGGCCCTCACTATTTGGTGATCCCGGTGAGGGACAAGACGTGATATGGTTAAGCCATTGGAAGTTTCGAAACGATTTGGAAGGTGGCGATGAGGTGACTGTTTCAGTTATGACAATATCTGAGTTTCAGGTAAAGGAGTTTGGCATCCAGATTGTGTACCACGAGCAAGAAGACACGATGTTGAGCACCCAGGACAACACCACAGATGACTTTTTCTATCCAGTTGTCATTGGTGGAGATTTGTCACGTTTTCTGCGGTCAGGATCAGGAACATACCTTCTTCGCCATGGGCTAAATTGGCTAACAGAGGAAGAAAAGGATTGTGTGTTCAGTTCAGTTTTGTTCCAGGACGACATTGGAGACCATAATAAACTAACAGGCAGATGTTGGAAGGTGGTAATGATCGTAGCCGCTATCTTCTTTCTCTCTCTTCTTCTGGTCTCTTGCACCTCTATTTCAGTGAAAGATCAGTTAATCCAGTTATTCAGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1194

Amino Acids

135.48

Weight (kDa)

6.22

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 187 1.4e-51 TIR domain
TIR_2 PF13676 21 - 132 1.5e-14 TIR domain
NB-ARC PF00931 201 - 370 2.1e-19 NB-ARC domain
WHD_ROQ1 PF23282 440 - 510 1.1e-17 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2490
Acc36I ACCTGC 1 cut(s) 2934
AccB1I GGYRCC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 2331
AccII CGCG 2 cut(s) 1500, 2110
AciI CCGC 8 cut(s) 556, 1498, 2108, 2256, 2635, 2798, 3343, 3498
AclI AACGTT 1 cut(s) 146
AcsI RAATTY 8 cut(s) 701, 718, 1389, 1772, 1808, 2295, 2406, 2536
AcuI CTGAAG 2 cut(s) 474, 1075
AfiI CCNNNNNNNGG 9 cut(s) 1014, 1504, 2531, 2610, 2790, 2816, 2876, 2943, 3116
AflII CTTAAG 1 cut(s) 2335
AgeI ACCGGT 1 cut(s) 2667
AhdI GACNNNNNGTC 1 cut(s) 253
AjiI CACGTC 1 cut(s) 3127
AjnI CCWGG 9 cut(s) 190, 858, 979, 1540, 2181, 2778, 2814, 3276, 3432
AjuI GAANNNNNNNTTGG 2 cut(s) 2930, 2962
AloI GAACNNNNNNTCC 2 cut(s) 3398, 3430
Alw21I GWGCWC 2 cut(s) 1089, 3275
Alw26I GTCTC 7 cut(s) 357, 1050, 1688, 1879, 2483, 3441, 3532
AlwNI CAGNNNCTG 1 cut(s) 2821
Ama87I CYCGRG 2 cut(s) 600, 2301
AoxI GGCC 2 cut(s) 2781, 3089
ApaI GGGCCC 1 cut(s) 3093
ApeKI GCWGC 2 cut(s) 458, 1878
ApoI RAATTY 8 cut(s) 701, 718, 1389, 1772, 1808, 2295, 2406, 2536
ArsI GACNNNNNNTTYG 2 cut(s) 1961, 1993
AsiGI ACCGGT 1 cut(s) 2667
Asp700I GAANNNNTTC 3 cut(s) 595, 1260, 2963
AspS9I GGNCC 3 cut(s) 2904, 3089, 3090
AsuC2I CCSGG 2 cut(s) 2856, 3111
AsuHPI GGTGA 8 cut(s) 439, 959, 1022, 2584, 2608, 3116, 3125, 3193
AsuII TTCGAA 2 cut(s) 142, 3154
AvaI CYCGRG 2 cut(s) 600, 2301
AvaII GGWCC 1 cut(s) 2904
BaeGI GKGCMC 2 cut(s) 504, 3093
BanI GGYRCC 1 cut(s) 267
BanII GRGCYC 2 cut(s) 1089, 3093
BarI GAAGNNNNNNTAC 2 cut(s) 2380, 2412
BauI CACGAG 2 cut(s) 503, 3248
BbsI GAAGAC 5 cut(s) 249, 311, 325, 549, 3264
Bbv12I GWGCWC 2 cut(s) 1089, 3275
BbvI GCAGC 2 cut(s) 445, 1890
BccI CCATC 8 cut(s) 215, 491, 1785, 1924, 2191, 2219, 2819, 2993
BceAI ACGGC 2 cut(s) 799, 2012
BciT130I CCWGG 9 cut(s) 192, 860, 981, 1542, 2183, 2780, 2816, 3278, 3434
BclI TGATCA 3 cut(s) 1324, 1678, 2709
BcnI CCSGG 2 cut(s) 2856, 3111
BcoDI GTCTC 7 cut(s) 357, 1050, 1688, 1879, 2483, 3441, 3532
BfaI CTAG 5 cut(s) 36, 914, 1394, 1991, 2054
BfmI CTRYAG 4 cut(s) 459, 768, 1873, 2529
BfrI CTTAAG 1 cut(s) 2335
BfuAI ACCTGC 1 cut(s) 2934
BglII AGATCT 2 cut(s) 463, 2413
BisI GCNGC 4 cut(s) 459, 1879, 2256, 3498
BlsI GCNGC 4 cut(s) 460, 1880, 2257, 3499
Bme18I GGWCC 1 cut(s) 2904
BmeRI GACNNNNNGTC 1 cut(s) 253
BmeT110I CYCGRG 2 cut(s) 600, 2301
BmgBI CACGTC 1 cut(s) 3127
BmgT120I GGNCC 3 cut(s) 2904, 3089, 3090
BmiI GGNNCC 6 cut(s) 269, 1539, 2868, 2905, 2948, 3091
BmrI ACTGGG 2 cut(s) 1824, 2761
BmsI GCATC 9 cut(s) 621, 951, 1434, 1473, 1495, 1573, 1704, 1751, 3240
BmuI ACTGGG 2 cut(s) 1824, 2761
BpiI GAAGAC 5 cut(s) 249, 311, 325, 549, 3264
BplI GAGNNNNNCTC 2 cut(s) 891, 923
BpmI CTGGAG 2 cut(s) 1718, 2587
Bpu14I TTCGAA 2 cut(s) 142, 3154
BpuEI CTTGAG 4 cut(s) 88, 188, 284, 1903
BpuMI CCSGG 2 cut(s) 2856, 3111
Bsa29I ATCGAT 2 cut(s) 634, 2242
BsaAI YACGTR 2 cut(s) 565, 1747
BsaBI GATNNNNATC 3 cut(s) 345, 590, 933
BsaI GGTCTC 3 cut(s) 1688, 3441, 3532
BsaWI WCCGGW 3 cut(s) 2233, 2443, 2667
BsaXI ACNNNNNCTCC 2 cut(s) 2648, 2678
Bsc4I CCNNNNNNNGG 9 cut(s) 1014, 1504, 2531, 2610, 2790, 2816, 2876, 2943, 3116
Bse118I RCCGGY 1 cut(s) 2667
Bse1I ACTGG 8 cut(s) 329, 1185, 1257, 1830, 2767, 3015, 3308, 3565
Bse3DI GCAATG 2 cut(s) 2644, 2998
Bse8I GATNNNNATC 3 cut(s) 345, 590, 933
BseBI CCWGG 9 cut(s) 192, 860, 981, 1542, 2183, 2780, 2816, 3278, 3434
BseCI ATCGAT 2 cut(s) 634, 2242
BseGI GGATG 6 cut(s) 207, 966, 1425, 1766, 2811, 3231
BseJI GATNNNNATC 3 cut(s) 345, 590, 933
BseLI CCNNNNNNNGG 9 cut(s) 1014, 1504, 2531, 2610, 2790, 2816, 2876, 2943, 3116
BseMI GCAATG 2 cut(s) 2644, 2998
BseMII CTCAG 3 cut(s) 1896, 2182, 3198
BseNI ACTGG 8 cut(s) 329, 1185, 1257, 1830, 2767, 3015, 3308, 3565
BseRI GAGGAG 2 cut(s) 1721, 2276
BseSI GKGCMC 2 cut(s) 504, 3093
BseXI GCAGC 2 cut(s) 445, 1890
BseYI CCCAGC 2 cut(s) 1013, 1648
BsgI GTGCAG 2 cut(s) 70, 1499
Bsh1236I CGCG 2 cut(s) 1500, 2110
BshFI GGCC 2 cut(s) 2783, 3091
BshNI GGYRCC 1 cut(s) 267
BshTI ACCGGT 1 cut(s) 2667
BshVI ATCGAT 2 cut(s) 634, 2242
BsiHKAI GWGCWC 2 cut(s) 1089, 3275
BsiHKCI CYCGRG 2 cut(s) 600, 2301
BsiSI CCGG 5 cut(s) 2234, 2444, 2668, 2855, 3111
BslFI GGGAC 6 cut(s) 1967, 2073, 2749, 2793, 2832, 3132
BslI CCNNNNNNNGG 9 cut(s) 1014, 1504, 2531, 2610, 2790, 2816, 2876, 2943, 3116
BsmAI GTCTC 7 cut(s) 357, 1050, 1688, 1879, 2483, 3441, 3532
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 6 cut(s) 1967, 2073, 2749, 2793, 2832, 3132
BsmI GAATGC 1 cut(s) 675
BsnI GGCC 2 cut(s) 2783, 3091
Bso31I GGTCTC 3 cut(s) 1688, 3441, 3532
BsoBI CYCGRG 2 cut(s) 600, 2301
Bsp119I TTCGAA 2 cut(s) 142, 3154
Bsp120I GGGCCC 1 cut(s) 3089
Bsp1286I GDGCHC 4 cut(s) 504, 1089, 3093, 3275
Bsp19I CCATGG 2 cut(s) 693, 3373
BspACI CCGC 8 cut(s) 556, 1498, 2108, 2256, 2635, 2798, 3343, 3498
BspANI GGCC 2 cut(s) 2783, 3091
BspCNI CTCAG 3 cut(s) 1897, 2181, 3199
BspDI ATCGAT 2 cut(s) 634, 2242
BspFNI CGCG 2 cut(s) 1500, 2110
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 6 cut(s) 269, 1539, 2868, 2905, 2948, 3091
BspMAI CTGCAG 2 cut(s) 463, 772
BspMI ACCTGC 1 cut(s) 2934
BspQI GCTCTTC 1 cut(s) 1094
BspT104I TTCGAA 2 cut(s) 142, 3154
BspT107I GGYRCC 1 cut(s) 267
BspTI CTTAAG 1 cut(s) 2335
BspTNI GGTCTC 3 cut(s) 1688, 3441, 3532
BsrDI GCAATG 2 cut(s) 2644, 2998
BsrFI RCCGGY 1 cut(s) 2667
BsrI ACTGG 8 cut(s) 329, 1185, 1257, 1830, 2767, 3015, 3308, 3565
BssAI RCCGGY 1 cut(s) 2667
BssSI CACGAG 2 cut(s) 503, 3248
BssT1I CCWWGG 3 cut(s) 693, 2548, 3373
Bst2BI CACGAG 2 cut(s) 503, 3248
Bst2UI CCWGG 9 cut(s) 192, 860, 981, 1542, 2183, 2780, 2816, 3278, 3434
Bst4CI ACNGT 7 cut(s) 216, 427, 743, 1687, 2530, 2923, 3187
Bst6I CTCTTC 3 cut(s) 1094, 1504, 3522
BstAFI CTTAAG 1 cut(s) 2335
BstBAI YACGTR 2 cut(s) 565, 1747
BstBI TTCGAA 2 cut(s) 142, 3154
BstC8I GCNNGC 1 cut(s) 1351
BstDEI CTNAG 8 cut(s) 551, 1295, 1739, 1905, 1937, 2168, 2342, 3207
BstDSI CCRYGG 2 cut(s) 693, 3373
BstF5I GGATG 6 cut(s) 207, 966, 1425, 1766, 2811, 3231
BstFNI CGCG 2 cut(s) 1500, 2110
BstMAI GTCTC 7 cut(s) 357, 1050, 1688, 1879, 2483, 3441, 3532
BstMWI GCNNNNNNNGC 8 cut(s) 11, 458, 467, 1878, 1996, 2841, 2886, 3385
BstNI CCWGG 9 cut(s) 192, 860, 981, 1542, 2183, 2780, 2816, 3278, 3434
BstNSI RCATGY 4 cut(s) 1103, 1353, 1639, 2176
BstSFI CTRYAG 4 cut(s) 459, 768, 1873, 2529
BstSLI GKGCMC 2 cut(s) 504, 3093
BstSNI TACGTA 1 cut(s) 1747
BstUI CGCG 2 cut(s) 1500, 2110
BstV1I GCAGC 2 cut(s) 445, 1890
BstV2I GAAGAC 5 cut(s) 249, 311, 325, 549, 3264
BstX2I RGATCY 4 cut(s) 463, 1926, 2413, 3081
BstYI RGATCY 4 cut(s) 463, 1926, 2413, 3081
Bsu15I ATCGAT 2 cut(s) 634, 2242
BsuRI GGCC 2 cut(s) 2783, 3091
BsuTUI ATCGAT 2 cut(s) 634, 2242
BtgI CCRYGG 2 cut(s) 693, 3373
BtgZI GCGATG 1 cut(s) 3189
BtrI CACGTC 1 cut(s) 3127
BtsCI GGATG 6 cut(s) 207, 966, 1425, 1766, 2811, 3231
BtsI GCAGTG 1 cut(s) 2250
BtsIMutI CAGTG 5 cut(s) 1122, 1178, 1241, 2250, 3552
BveI ACCTGC 1 cut(s) 2934
Cac8I GCNNGC 1 cut(s) 1351
CaiI CAGNNNCTG 1 cut(s) 2821
CciI TCATGA 1 cut(s) 814
Cfr10I RCCGGY 1 cut(s) 2667
Cfr13I GGNCC 3 cut(s) 2904, 3089, 3090
ClaI ATCGAT 2 cut(s) 634, 2242
CseI GACGC 2 cut(s) 243, 568
CspAI ACCGGT 1 cut(s) 2667
CspCI CAANNNNNGTGG 2 cut(s) 1134, 1169
DdeI CTNAG 8 cut(s) 551, 1295, 1739, 1905, 1937, 2168, 2342, 3207
DraI TTTAAA 2 cut(s) 1807, 2073
DriI GACNNNNNGTC 1 cut(s) 253
Eam1104I CTCTTC 3 cut(s) 1094, 1504, 3522
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 3 cut(s) 1094, 1504, 3522
EciI GGCGGA 1 cut(s) 571
Ecl136II GAGCTC 1 cut(s) 1087
Eco105I TACGTA 1 cut(s) 1747
Eco130I CCWWGG 3 cut(s) 693, 2548, 3373
Eco24I GRGCYC 2 cut(s) 1089, 3093
Eco31I GGTCTC 3 cut(s) 1688, 3441, 3532
Eco47I GGWCC 1 cut(s) 2904
Eco53kI GAGCTC 1 cut(s) 1087
Eco57I CTGAAG 2 cut(s) 474, 1075
Eco88I CYCGRG 2 cut(s) 600, 2301
EcoICRI GAGCTC 1 cut(s) 1087
EcoO109I RGGNCCY 2 cut(s) 2904, 3090
EcoRI GAATTC 1 cut(s) 1389
EcoRII CCWGG 9 cut(s) 190, 858, 979, 1540, 2181, 2778, 2814, 3276, 3432
EcoT14I CCWWGG 3 cut(s) 693, 2548, 3373
EcoT22I ATGCAT 2 cut(s) 675, 1488
EcoT38I GRGCYC 2 cut(s) 1089, 3093
ErhI CCWWGG 3 cut(s) 693, 2548, 3373
Esp3I CGTCTC 1 cut(s) 357
FaqI GGGAC 6 cut(s) 1967, 2073, 2749, 2793, 2832, 3132
FauI CCCGC 1 cut(s) 2115
FbaI TGATCA 3 cut(s) 1324, 1678, 2709
FblI GTMKAC 1 cut(s) 2331
Fnu4HI GCNGC 4 cut(s) 459, 1879, 2256, 3498
FokI GGATG 6 cut(s) 194, 973, 1412, 1773, 2798, 3218
FriOI GRGCYC 2 cut(s) 1089, 3093
Fsp4HI GCNGC 4 cut(s) 459, 1879, 2256, 3498
FspBI CTAG 5 cut(s) 36, 914, 1394, 1991, 2054
GluI GCNGC 4 cut(s) 459, 1879, 2256, 3498
GsaI CCCAGC 2 cut(s) 1017, 1652
GsuI CTGGAG 2 cut(s) 1718, 2587
HaeIII GGCC 2 cut(s) 2783, 3091
HapII CCGG 5 cut(s) 2234, 2444, 2668, 2855, 3111
HgaI GACGC 2 cut(s) 243, 568
HincII GTYRAC 3 cut(s) 130, 925, 2035
HindII GTYRAC 3 cut(s) 130, 925, 2035
HindIII AAGCTT 1 cut(s) 12
HpaI GTTAAC 1 cut(s) 130
HpaII CCGG 5 cut(s) 2234, 2444, 2668, 2855, 3111
HphI GGTGA 8 cut(s) 439, 959, 1022, 2584, 2608, 3116, 3125, 3193
Hpy99I CGWCG 1 cut(s) 2808
HpyAV CCTTC 8 cut(s) 424, 1143, 1613, 1891, 2788, 3161, 3374, 3472
HpyCH4III ACNGT 7 cut(s) 216, 427, 743, 1687, 2530, 2923, 3187
HpyCH4IV ACGT 7 cut(s) 146, 564, 1549, 1746, 2846, 3126, 3334
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 458, 467, 1878, 1996, 2841, 2886, 3385
HpyF3I CTNAG 8 cut(s) 551, 1295, 1739, 1905, 1937, 2168, 2342, 3207
HpySE526I ACGT 7 cut(s) 146, 564, 1549, 1746, 2846, 3126, 3334
Ksp22I TGATCA 3 cut(s) 1324, 1678, 2709
KspAI GTTAAC 1 cut(s) 130
LguI GCTCTTC 1 cut(s) 1094
LmnI GCTCC 2 cut(s) 1084, 2109
Lsp1109I GCAGC 2 cut(s) 445, 1890
LweI GCATC 9 cut(s) 621, 951, 1434, 1473, 1495, 1573, 1704, 1751, 3240
MaeI CTAG 5 cut(s) 36, 914, 1394, 1991, 2054
MaeII ACGT 7 cut(s) 146, 564, 1549, 1746, 2846, 3126, 3334
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 4 cut(s) 463, 1926, 2413, 3081
MhlI GDGCHC 4 cut(s) 504, 1089, 3093, 3275
MlyI GAGTC 1 cut(s) 1298
MmeI TCCRAC 4 cut(s) 235, 336, 2771, 3455
Mph1103I ATGCAT 2 cut(s) 675, 1488
MroXI GAANNNNTTC 3 cut(s) 595, 1260, 2963
MslI CAYNNNNRTG 2 cut(s) 1640, 1947
MspCI CTTAAG 1 cut(s) 2335
MspI CCGG 5 cut(s) 2234, 2444, 2668, 2855, 3111
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 675
MvaI CCWGG 9 cut(s) 192, 860, 981, 1542, 2183, 2780, 2816, 3278, 3434
MvnI CGCG 2 cut(s) 1500, 2110
MwoI GCNNNNNNNGC 8 cut(s) 11, 458, 467, 1878, 1996, 2841, 2886, 3385
NciI CCSGG 2 cut(s) 2856, 3111
NcoI CCATGG 2 cut(s) 693, 3373
NlaIV GGNNCC 6 cut(s) 269, 1539, 2868, 2905, 2948, 3091
NmeAIII GCCGAG 1 cut(s) 2284
NmuCI GTSAC 7 cut(s) 216, 1453, 1949, 2266, 2572, 3181, 3330
NsiI ATGCAT 2 cut(s) 675, 1488
NspI RCATGY 4 cut(s) 1103, 1353, 1639, 2176
NspV TTCGAA 2 cut(s) 142, 3154
PaeI GCATGC 1 cut(s) 1353
PaeR7I CTCGAG 1 cut(s) 600
PagI TCATGA 1 cut(s) 814
PciSI GCTCTTC 1 cut(s) 1094
PcsI WCGNNNNNNNCGW 2 cut(s) 148, 2947
PctI GAATGC 1 cut(s) 675
PdmI GAANNNNTTC 3 cut(s) 595, 1260, 2963
PflFI GACNNNGTC 1 cut(s) 1382
PfoI TCCNGGA 2 cut(s) 979, 3432
PinAI ACCGGT 1 cut(s) 2667
PkrI GCNGC 4 cut(s) 460, 1880, 2257, 3499
PleI GAGTC 1 cut(s) 1298
PpsI GAGTC 1 cut(s) 1298
Ppu21I YACGTR 2 cut(s) 565, 1747
PpuMI RGGWCCY 1 cut(s) 2904
PsiI TTATAA 1 cut(s) 2490
Psp124BI GAGCTC 1 cut(s) 1089
Psp1406I AACGTT 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 2904
Psp6I CCWGG 9 cut(s) 190, 858, 979, 1540, 2181, 2778, 2814, 3276, 3432
PspFI CCCAGC 2 cut(s) 1013, 1648
PspGI CCWGG 9 cut(s) 190, 858, 979, 1540, 2181, 2778, 2814, 3276, 3432
PspN4I GGNNCC 6 cut(s) 269, 1539, 2868, 2905, 2948, 3091
PspOMI GGGCCC 1 cut(s) 3089
PspPI GGNCC 3 cut(s) 2904, 3089, 3090
PspPPI RGGWCCY 1 cut(s) 2904
PstI CTGCAG 2 cut(s) 463, 772
PstNI CAGNNNCTG 1 cut(s) 2821
PsuI RGATCY 4 cut(s) 463, 1926, 2413, 3081
PsyI GACNNNGTC 1 cut(s) 1382
RseI CAYNNNNRTG 2 cut(s) 1640, 1947
SacI GAGCTC 1 cut(s) 1089
SapI GCTCTTC 1 cut(s) 1094
SatI GCNGC 4 cut(s) 459, 1879, 2256, 3498
Sau96I GGNCC 3 cut(s) 2904, 3089, 3090
SchI GAGTC 1 cut(s) 1298
SduI GDGCHC 4 cut(s) 504, 1089, 3093, 3275
SfaNI GCATC 9 cut(s) 621, 951, 1434, 1473, 1495, 1573, 1704, 1751, 3240
SfcI CTRYAG 4 cut(s) 459, 768, 1873, 2529
Sfr274I CTCGAG 1 cut(s) 600
SfuI TTCGAA 2 cut(s) 142, 3154
SinI GGWCC 1 cut(s) 2904
SlaI CTCGAG 1 cut(s) 600
SmiI ATTTAAAT 1 cut(s) 2073
SmiMI CAYNNNNRTG 2 cut(s) 1640, 1947
SmlI CTYRAG 6 cut(s) 67, 167, 299, 600, 1882, 2335
SmoI CTYRAG 6 cut(s) 67, 167, 299, 600, 1882, 2335
SnaBI TACGTA 1 cut(s) 1747
SphI GCATGC 1 cut(s) 1353
SsiI CCGC 8 cut(s) 556, 1498, 2108, 2256, 2635, 2798, 3343, 3498
SspI AATATT 4 cut(s) 729, 2116, 2741, 3042
SspMI CTAG 5 cut(s) 36, 914, 1394, 1991, 2054
SstI GAGCTC 1 cut(s) 1089
StyI CCWWGG 3 cut(s) 693, 2548, 3373
SwaI ATTTAAAT 1 cut(s) 2073
TaaI ACNGT 7 cut(s) 216, 427, 743, 1687, 2530, 2923, 3187
TaiI ACGT 7 cut(s) 149, 567, 1552, 1749, 2849, 3129, 3337
TaqI TCGA 6 cut(s) 142, 601, 634, 788, 2242, 3154
TatI WGTACW 3 cut(s) 604, 1238, 2929
TauI GCSGC 2 cut(s) 2258, 3500
TscAI CASTG 5 cut(s) 1129, 1185, 1248, 2257, 3552
TseFI GTSAC 7 cut(s) 216, 1453, 1949, 2266, 2572, 3181, 3330
TseI GCWGC 2 cut(s) 458, 1878
Tsp45I GTSAC 7 cut(s) 216, 1453, 1949, 2266, 2572, 3181, 3330
TspDTI ATGAA 6 cut(s) 177, 504, 803, 1089, 2018, 2057
TspGWI ACGGA 3 cut(s) 235, 339, 567
TspRI CASTG 5 cut(s) 1129, 1185, 1248, 2257, 3552
Tth111I GACNNNGTC 1 cut(s) 1382
Vha464I CTTAAG 1 cut(s) 2335
VpaK11BI GGWCC 1 cut(s) 2904
XapI RAATTY 8 cut(s) 701, 718, 1389, 1772, 1808, 2295, 2406, 2536
XbaI TCTAGA 2 cut(s) 913, 1393
XceI RCATGY 4 cut(s) 1103, 1353, 1639, 2176
XcmI CCANNNNNNNNNTGG 2 cut(s) 2897, 3440
XhoI CTCGAG 1 cut(s) 600
XmiI GTMKAC 1 cut(s) 2331
XmnI GAANNNNTTC 3 cut(s) 595, 1260, 2963
XspI CTAG 5 cut(s) 36, 914, 1394, 1991, 2054
Zsp2I ATGCAT 2 cut(s) 675, 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.