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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 500 |
| Acc16I | TGCGCA | 1 cut(s) | 1217 |
| AccB1I | GGYRCC | 1 cut(s) | 1068 |
| AccI | GTMKAC | 1 cut(s) | 157 |
| AclWI | GGATC | 2 cut(s) | 148, 1424 |
| AcsI | RAATTY | 1 cut(s) | 223 |
| AcyI | GRCGYC | 1 cut(s) | 1069 |
| AfaI | GTAC | 5 cut(s) | 8, 456, 551, 1092, 1111 |
| AflII | CTTAAG | 1 cut(s) | 1381 |
| AflIII | ACRYGT | 2 cut(s) | 208, 1327 |
| AjnI | CCWGG | 1 cut(s) | 1134 |
| AluBI | AGCT | 6 cut(s) | 234, 396, 589, 686, 754, 1380 |
| AluI | AGCT | 6 cut(s) | 234, 396, 589, 686, 754, 1380 |
| Alw21I | GWGCWC | 1 cut(s) | 532 |
| Alw26I | GTCTC | 2 cut(s) | 42, 1175 |
| AlwI | GGATC | 2 cut(s) | 148, 1424 |
| AoxI | GGCC | 1 cut(s) | 289 |
| ApoI | RAATTY | 1 cut(s) | 223 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 836, 1023 |
| AspLEI | GCGC | 3 cut(s) | 389, 1071, 1218 |
| AspS9I | GGNCC | 3 cut(s) | 430, 439, 616 |
| AsuC2I | CCSGG | 1 cut(s) | 483 |
| AsuHPI | GGTGA | 3 cut(s) | 374, 785, 967 |
| AsuII | TTCGAA | 1 cut(s) | 1086 |
| AvaII | GGWCC | 3 cut(s) | 430, 439, 616 |
| BanI | GGYRCC | 1 cut(s) | 1068 |
| BbsI | GAAGAC | 1 cut(s) | 197 |
| Bbv12I | GWGCWC | 1 cut(s) | 532 |
| BccI | CCATC | 6 cut(s) | 131, 893, 959, 1242, 1284, 1427 |
| BciT130I | CCWGG | 1 cut(s) | 1136 |
| BciVI | GTATCC | 1 cut(s) | 962 |
| BcnI | CCSGG | 1 cut(s) | 483 |
| BcoDI | GTCTC | 2 cut(s) | 42, 1175 |
| BfaI | CTAG | 4 cut(s) | 293, 399, 507, 584 |
| BfoI | RGCGCY | 2 cut(s) | 390, 1072 |
| BfrI | CTTAAG | 1 cut(s) | 1381 |
| BfuI | GTATCC | 1 cut(s) | 962 |
| BmcAI | AGTACT | 2 cut(s) | 456, 1092 |
| Bme1390I | CCNGG | 2 cut(s) | 483, 1136 |
| Bme18I | GGWCC | 3 cut(s) | 430, 439, 616 |
| BmgT120I | GGNCC | 3 cut(s) | 430, 439, 616 |
| BmiI | GGNNCC | 1 cut(s) | 1070 |
| BmrFI | CCNGG | 2 cut(s) | 483, 1136 |
| BmsI | GCATC | 5 cut(s) | 126, 439, 938, 1159, 1203 |
| BpiI | GAAGAC | 1 cut(s) | 197 |
| BpmI | CTGGAG | 1 cut(s) | 201 |
| Bpu14I | TTCGAA | 1 cut(s) | 1086 |
| BpuMI | CCSGG | 1 cut(s) | 483 |
| Bsa29I | ATCGAT | 1 cut(s) | 999 |
| BsaBI | GATNNNNATC | 1 cut(s) | 95 |
| BsaHI | GRCGYC | 1 cut(s) | 1069 |
| BsaI | GGTCTC | 1 cut(s) | 1175 |
| BsaJI | CCNNGG | 1 cut(s) | 1134 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1215, 1245 |
| Bse3DI | GCAATG | 2 cut(s) | 198, 846 |
| Bse8I | GATNNNNATC | 1 cut(s) | 95 |
| BseBI | CCWGG | 1 cut(s) | 1136 |
| BseCI | ATCGAT | 1 cut(s) | 999 |
| BseDI | CCNNGG | 1 cut(s) | 1134 |
| BseGI | GGATG | 5 cut(s) | 733, 762, 908, 1218, 1265 |
| BseJI | GATNNNNATC | 1 cut(s) | 95 |
| BseMI | GCAATG | 2 cut(s) | 198, 846 |
| BseMII | CTCAG | 1 cut(s) | 1085 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1003 |
| BshFI | GGCC | 1 cut(s) | 291 |
| BshNI | GGYRCC | 1 cut(s) | 1068 |
| BshVI | ATCGAT | 1 cut(s) | 999 |
| BsiEI | CGRYCG | 1 cut(s) | 1003 |
| BsiHKAI | GWGCWC | 1 cut(s) | 532 |
| BsiSI | CCGG | 1 cut(s) | 483 |
| BslFI | GGGAC | 2 cut(s) | 606, 884 |
| BsmAI | GTCTC | 2 cut(s) | 42, 1175 |
| BsmFI | GGGAC | 2 cut(s) | 606, 884 |
| BsmI | GAATGC | 1 cut(s) | 643 |
| BsnI | GGCC | 1 cut(s) | 291 |
| Bso31I | GGTCTC | 1 cut(s) | 1175 |
| Bsp119I | TTCGAA | 1 cut(s) | 1086 |
| Bsp1286I | GDGCHC | 1 cut(s) | 532 |
| Bsp1407I | TGTACA | 1 cut(s) | 549 |
| Bsp143I | GATC | 8 cut(s) | 140, 277, 330, 469, 996, 1000, 1333, 1429 |
| BspANI | GGCC | 1 cut(s) | 291 |
| BspCNI | CTCAG | 1 cut(s) | 1086 |
| BspDI | ATCGAT | 1 cut(s) | 999 |
| BspHI | TCATGA | 1 cut(s) | 780 |
| BspLI | GGNNCC | 1 cut(s) | 1070 |
| BspPI | GGATC | 2 cut(s) | 148, 1424 |
| BspT104I | TTCGAA | 1 cut(s) | 1086 |
| BspT107I | GGYRCC | 1 cut(s) | 1068 |
| BspTI | CTTAAG | 1 cut(s) | 1381 |
| BspTNI | GGTCTC | 1 cut(s) | 1175 |
| BsrDI | GCAATG | 2 cut(s) | 198, 846 |
| BsrGI | TGTACA | 1 cut(s) | 549 |
| BssECI | CCNNGG | 1 cut(s) | 1134 |
| BssMI | GATC | 8 cut(s) | 140, 277, 330, 469, 996, 1000, 1333, 1429 |
| BssNAI | GTATAC | 1 cut(s) | 158 |
| BssNI | GRCGYC | 1 cut(s) | 1069 |
| Bst1107I | GTATAC | 1 cut(s) | 158 |
| Bst2UI | CCWGG | 1 cut(s) | 1136 |
| Bst4CI | ACNGT | 4 cut(s) | 358, 620, 809, 1186 |
| Bst6I | CTCTTC | 2 cut(s) | 1009, 1035 |
| BstACI | GRCGYC | 1 cut(s) | 1069 |
| BstAFI | CTTAAG | 1 cut(s) | 1381 |
| BstAUI | TGTACA | 1 cut(s) | 549 |
| BstBI | TTCGAA | 1 cut(s) | 1086 |
| BstC8I | GCNNGC | 1 cut(s) | 603 |
| BstDEI | CTNAG | 4 cut(s) | 524, 791, 1094, 1238 |
| BstF5I | GGATG | 5 cut(s) | 733, 762, 908, 1218, 1265 |
| BstH2I | RGCGCY | 2 cut(s) | 390, 1072 |
| BstHHI | GCGC | 3 cut(s) | 389, 1071, 1218 |
| BstKTI | GATC | 8 cut(s) | 143, 280, 333, 472, 999, 1003, 1336, 1432 |
| BstMAI | GTCTC | 2 cut(s) | 42, 1175 |
| BstMBI | GATC | 8 cut(s) | 140, 277, 330, 469, 996, 1000, 1333, 1429 |
| BstMCI | CGRYCG | 1 cut(s) | 1003 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 393 |
| BstNI | CCWGG | 1 cut(s) | 1136 |
| BstNSI | RCATGY | 4 cut(s) | 178, 212, 605, 1331 |
| BstSCI | CCNGG | 2 cut(s) | 481, 1134 |
| BstV2I | GAAGAC | 1 cut(s) | 197 |
| BstX2I | RGATCY | 1 cut(s) | 1429 |
| BstYI | RGATCY | 1 cut(s) | 1429 |
| BstZ17I | GTATAC | 1 cut(s) | 158 |
| Bsu15I | ATCGAT | 1 cut(s) | 999 |
| BsuI | GTATCC | 1 cut(s) | 962 |
| BsuRI | GGCC | 1 cut(s) | 291 |
| BsuTUI | ATCGAT | 1 cut(s) | 999 |
| BtsCI | GGATG | 5 cut(s) | 733, 762, 908, 1218, 1265 |
| Cac8I | GCNNGC | 1 cut(s) | 603 |
| CciI | TCATGA | 1 cut(s) | 780 |
| CfoI | GCGC | 3 cut(s) | 389, 1071, 1218 |
| Cfr13I | GGNCC | 3 cut(s) | 430, 439, 616 |
| ClaI | ATCGAT | 1 cut(s) | 999 |
| CseI | GACGC | 1 cut(s) | 658 |
| Csp6I | GTAC | 5 cut(s) | 7, 455, 550, 1091, 1110 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 833, 868 |
| CviJI | RGCY | 8 cut(s) | 234, 291, 396, 589, 686, 754, 1303, 1380 |
| CviKI_1 | RGCY | 8 cut(s) | 234, 291, 396, 589, 686, 754, 1303, 1380 |
| CviQI | GTAC | 5 cut(s) | 7, 455, 550, 1091, 1110 |
| DdeI | CTNAG | 4 cut(s) | 524, 791, 1094, 1238 |
| DinI | GGCGCC | 1 cut(s) | 1070 |
| DpnI | GATC | 8 cut(s) | 142, 279, 332, 471, 998, 1002, 1335, 1431 |
| DpnII | GATC | 8 cut(s) | 140, 277, 330, 469, 996, 1000, 1333, 1429 |
| DraI | TTTAAA | 1 cut(s) | 238 |
| Eam1104I | CTCTTC | 2 cut(s) | 1009, 1035 |
| EarI | CTCTTC | 2 cut(s) | 1009, 1035 |
| Eco31I | GGTCTC | 1 cut(s) | 1175 |
| Eco47I | GGWCC | 3 cut(s) | 430, 439, 616 |
| EcoRII | CCWGG | 1 cut(s) | 1134 |
| EcoT22I | ATGCAT | 5 cut(s) | 607, 990, 1146, 1152, 1204 |
| EgeI | GGCGCC | 1 cut(s) | 1070 |
| EheI | GGCGCC | 1 cut(s) | 1070 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 396, 428 |
| FaqI | GGGAC | 2 cut(s) | 606, 884 |
| FauNDI | CATATG | 2 cut(s) | 435, 1146 |
| FblI | GTMKAC | 1 cut(s) | 157 |
| FokI | GGATG | 5 cut(s) | 740, 749, 915, 1225, 1272 |
| FspBI | CTAG | 4 cut(s) | 293, 399, 507, 584 |
| FspI | TGCGCA | 1 cut(s) | 1217 |
| GlaI | GCGC | 3 cut(s) | 388, 1070, 1217 |
| GsuI | CTGGAG | 1 cut(s) | 201 |
| HaeII | RGCGCY | 2 cut(s) | 390, 1072 |
| HaeIII | GGCC | 1 cut(s) | 291 |
| HapII | CCGG | 1 cut(s) | 483 |
| HgaI | GACGC | 1 cut(s) | 658 |
| HhaI | GCGC | 3 cut(s) | 389, 1071, 1218 |
| Hin1I | GRCGYC | 1 cut(s) | 1069 |
| Hin6I | GCGC | 3 cut(s) | 387, 1069, 1216 |
| HinP1I | GCGC | 3 cut(s) | 387, 1069, 1216 |
| HindIII | AAGCTT | 1 cut(s) | 232 |
| HinfI | GANTC | 7 cut(s) | 11, 96, 578, 1034, 1043, 1079, 1319 |
| HpaII | CCGG | 1 cut(s) | 483 |
| HphI | GGTGA | 3 cut(s) | 374, 785, 967 |
| Hpy166II | GTNNAC | 6 cut(s) | 158, 214, 430, 550, 812, 1112 |
| Hpy188I | TCNGA | 2 cut(s) | 674, 1039 |
| Hpy188III | TCNNGA | 8 cut(s) | 25, 180, 399, 781, 1004, 1047, 1361, 1393 |
| Hpy8I | GTNNAC | 6 cut(s) | 158, 214, 430, 550, 812, 1112 |
| Hpy99I | CGWCG | 1 cut(s) | 674 |
| HpyAV | CCTTC | 2 cut(s) | 861, 1351 |
| HpyCH4III | ACNGT | 4 cut(s) | 358, 620, 809, 1186 |
| HpyCH4IV | ACGT | 1 cut(s) | 1054 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 393 |
| HpyF3I | CTNAG | 4 cut(s) | 524, 791, 1094, 1238 |
| HpySE526I | ACGT | 1 cut(s) | 1054 |
| Hsp92I | GRCGYC | 1 cut(s) | 1069 |
| HspAI | GCGC | 3 cut(s) | 387, 1069, 1216 |
| KasI | GGCGCC | 1 cut(s) | 1068 |
| Kzo9I | GATC | 8 cut(s) | 140, 277, 330, 469, 996, 1000, 1333, 1429 |
| LmnI | GCTCC | 1 cut(s) | 640 |
| LpnPI | CCDG | 9 cut(s) | 165, 496, 599, 699, 842, 1032, 1121, 1148, 1378 |
| LweI | GCATC | 5 cut(s) | 126, 439, 938, 1159, 1203 |
| MaeI | CTAG | 4 cut(s) | 293, 399, 507, 584 |
| MaeII | ACGT | 1 cut(s) | 1054 |
| MaeIII | GTNAC | 4 cut(s) | 129, 538, 955, 1004 |
| MalI | GATC | 8 cut(s) | 142, 279, 332, 471, 998, 1002, 1335, 1431 |
| MboI | GATC | 8 cut(s) | 140, 277, 330, 469, 996, 1000, 1333, 1429 |
| MboII | GAAGA | 6 cut(s) | 202, 696, 1026, 1052, 1328, 1407 |
| MfeI | CAATTG | 2 cut(s) | 918, 960 |
| MflI | RGATCY | 1 cut(s) | 1429 |
| MhlI | GDGCHC | 1 cut(s) | 532 |
| Mly113I | GGCGCC | 1 cut(s) | 1069 |
| MlyI | GAGTC | 1 cut(s) | 1052 |
| MmeI | TCCRAC | 1 cut(s) | 652 |
| MnlI | CCTC | 3 cut(s) | 358, 851, 1089 |
| Mph1103I | ATGCAT | 5 cut(s) | 607, 990, 1146, 1152, 1204 |
| MroXI | GAANNNNTTC | 2 cut(s) | 836, 1023 |
| MseI | TTAA | 6 cut(s) | 36, 56, 237, 375, 1286, 1382 |
| MspCI | CTTAAG | 1 cut(s) | 1381 |
| MspI | CCGG | 1 cut(s) | 483 |
| MspR9I | CCNGG | 2 cut(s) | 483, 1136 |
| MunI | CAATTG | 2 cut(s) | 918, 960 |
| Mva1269I | GAATGC | 1 cut(s) | 643 |
| MvaI | CCWGG | 1 cut(s) | 1136 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 393 |
| NarI | GGCGCC | 1 cut(s) | 1069 |
| NciI | CCSGG | 1 cut(s) | 483 |
| NdeI | CATATG | 2 cut(s) | 435, 1146 |
| NdeII | GATC | 8 cut(s) | 140, 277, 330, 469, 996, 1000, 1333, 1429 |
| NlaIV | GGNNCC | 1 cut(s) | 1070 |
| NmuCI | GTSAC | 2 cut(s) | 955, 1004 |
| NsbI | TGCGCA | 1 cut(s) | 1217 |
| NsiI | ATGCAT | 5 cut(s) | 607, 990, 1146, 1152, 1204 |
| NspI | RCATGY | 4 cut(s) | 178, 212, 605, 1331 |
| NspV | TTCGAA | 1 cut(s) | 1086 |
| PaeI | GCATGC | 1 cut(s) | 605 |
| PagI | TCATGA | 1 cut(s) | 780 |
| PciI | ACATGT | 2 cut(s) | 208, 1327 |
| PctI | GAATGC | 1 cut(s) | 643 |
| PdmI | GAANNNNTTC | 2 cut(s) | 836, 1023 |
| PfeI | GAWTC | 6 cut(s) | 11, 96, 578, 1034, 1079, 1319 |
| PfoI | TCCNGGA | 1 cut(s) | 481 |
| Ple19I | CGATCG | 1 cut(s) | 1003 |
| PleI | GAGTC | 1 cut(s) | 1051 |
| PluTI | GGCGCC | 1 cut(s) | 1072 |
| PpsI | GAGTC | 1 cut(s) | 1051 |
| PscI | ACATGT | 2 cut(s) | 208, 1327 |
| PsiI | TTATAA | 1 cut(s) | 500 |
| Psp6I | CCWGG | 1 cut(s) | 1134 |
| PspGI | CCWGG | 1 cut(s) | 1134 |
| PspN4I | GGNNCC | 1 cut(s) | 1070 |
| PspPI | GGNCC | 3 cut(s) | 430, 439, 616 |
| PsuI | RGATCY | 1 cut(s) | 1429 |
| PvuI | CGATCG | 1 cut(s) | 1003 |
| RsaI | GTAC | 5 cut(s) | 8, 456, 551, 1092, 1111 |
| RsaNI | GTAC | 5 cut(s) | 7, 455, 550, 1091, 1110 |
| SaqAI | TTAA | 6 cut(s) | 36, 56, 237, 375, 1286, 1382 |
| Sau3AI | GATC | 8 cut(s) | 140, 277, 330, 469, 996, 1000, 1333, 1429 |
| Sau96I | GGNCC | 3 cut(s) | 430, 439, 616 |
| ScaI | AGTACT | 2 cut(s) | 456, 1092 |
| SchI | GAGTC | 1 cut(s) | 1052 |
| ScrFI | CCNGG | 2 cut(s) | 483, 1136 |
| SduI | GDGCHC | 1 cut(s) | 532 |
| SetI | ASST | 9 cut(s) | 236, 398, 549, 591, 688, 756, 1057, 1129, 1382 |
| SfaNI | GCATC | 5 cut(s) | 126, 439, 938, 1159, 1203 |
| SfoI | GGCGCC | 1 cut(s) | 1070 |
| SfuI | TTCGAA | 1 cut(s) | 1086 |
| SinI | GGWCC | 3 cut(s) | 430, 439, 616 |
| SmlI | CTYRAG | 1 cut(s) | 1381 |
| SmoI | CTYRAG | 1 cut(s) | 1381 |
| SphI | GCATGC | 1 cut(s) | 605 |
| SspDI | GGCGCC | 1 cut(s) | 1068 |
| SspMI | CTAG | 4 cut(s) | 293, 399, 507, 584 |
| StyD4I | CCNGG | 2 cut(s) | 481, 1134 |
| TaaI | ACNGT | 4 cut(s) | 358, 620, 809, 1186 |
| TaiI | ACGT | 1 cut(s) | 1057 |
| TaqI | TCGA | 4 cut(s) | 280, 897, 999, 1086 |
| TatI | WGTACW | 3 cut(s) | 454, 549, 1090 |
| TfiI | GAWTC | 6 cut(s) | 11, 96, 578, 1034, 1079, 1319 |
| Tru1I | TTAA | 6 cut(s) | 36, 56, 237, 375, 1286, 1382 |
| Tru9I | TTAA | 6 cut(s) | 36, 56, 237, 375, 1286, 1382 |
| TseFI | GTSAC | 2 cut(s) | 955, 1004 |
| Tsp45I | GTSAC | 2 cut(s) | 955, 1004 |
| TspDTI | ATGAA | 5 cut(s) | 241, 479, 591, 797, 1047 |
| Vha464I | CTTAAG | 1 cut(s) | 1381 |
| VpaK11BI | GGWCC | 3 cut(s) | 430, 439, 616 |
| XapI | RAATTY | 1 cut(s) | 223 |
| XbaI | TCTAGA | 1 cut(s) | 398 |
| XceI | RCATGY | 4 cut(s) | 178, 212, 605, 1331 |
| XmiI | GTMKAC | 1 cut(s) | 157 |
| XmnI | GAANNNNTTC | 2 cut(s) | 836, 1023 |
| XspI | CTAG | 4 cut(s) | 293, 399, 507, 584 |
| ZrmI | AGTACT | 2 cut(s) | 456, 1092 |
| Zsp2I | ATGCAT | 5 cut(s) | 607, 990, 1146, 1152, 1204 |