MD07G1103200.v1.1

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000036
Physical Location & Seq
Forward (+)
11693272 .. 11697556
4285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1103200.v1.1.491

Sequence Viewer

Length: 3045 bp
ATGGTCTTACAAAATCAAGCTGATGGGTATGAGTCAAAGTTTATCGAAAAAATTGTTAAAGTGATTGGAGGCAAGTTAGGTCGCACACCATTGAGCGTTGAACCAAAATTGATTGGAGTCAAATCTCAAGTCAAGGACATCAATTTGTGGTTACAAGATGGATCAACCGATGTCGGTATACTGGTTGTGTATGGCATGTCTGGAATAGGGAAGACAACCATTGCAAAACATGTTTACAATTCAAACTTTAGAAGCTTTGAACGAAGTAGTTTCATTGAAAATATCAGAGAAACAGCAAATCAACCAAATGGCTTGGTTCAAATACAAAAGCAACTTCTTTTTGATATTTTGAACGACATAGAAGTGAAAATACATGGTGTTAGTGAGGGACTAAGAAAGATTGAAAGAGCCATAAGCTCTAGAAGAGTGCTTCTTGTTCTTGATGATGTGGACCATATGGACCAGTTAGATGCAGTACTAGAGATGAAAGATCGATTTTATTCAGGAAGTAAGATACTTATAACAACTAGGCGTGAAAGGTTGCTAAAGGCACATCAAGTTACAAAGGTGCACAAAGTTGGAACTTTGTATTACAGTGAGTCATTAGAGCTTTTCAGTTGGCATGCTTTTCGCCAGGATCATCCCCTTAGAGGTTACATGGAATATTCAGAAAAGGTTGTACACTACTGCGACGGACTTCCATTAGCTCTAAAAGTTTTGGGCTCTTCTTTATCAGGGGAAAGTATAGATGTATGGAAAAGTGCATTGGAGAAGCTAAAAGTTATCCCTAATGGTGAAATCATGAATAAACTACGAATAAGCTATGACAGTTTACAAGATGACCATGACCGGGAATTATTCCTCCACATTGCTTGTTTCCTAATAGGAAGGAACAAAAGCCACATTGTTAGAATATTCGATGGATGTGATTTCTATACAATTGTTGGCATTCAAAATCTCATTGATAGATGCTTGGTGACAATTGATGGATGCAACAACGTGCAAATGCATGACATGATCCGTGACATGGGAAGAGAAATTGTTCGTCGCGAATCAGAAGATCCTGGAAAACGTAGTAGATTATGGCGCCATAAGGATTCTTTCGAAGTAATGAGGGAAAAGAATGGTACAAAAAAAATCCAAGGTCTTGTCCTGAATATGCGTATGCATCCTGCAAACAGTCCAATAAACACAACTGAGACAGTCTTGGAAACCAATGCATTTGAAAAGATGCGCAAATTACAACTACTACATCTTAGTCATGTACGACTGGATGGATGTTATGCAGATTTGCCTACAGGATTAAGATGGTTGTGTTGGCTTCAATTTCCTTTGGATTCTATACCTATTGAGTTTCCTTTGGGGTGTCTAATTGTTCTTGAAATGCAATACAGTAGATTGAGACAAGTCTGGAAAGGAAAAAAATTTCTTCCATCGTTGAAGATCCTTGACCTCAGCCATTCCCATGCCCTCACTGAAATCATGGACTTCTCACTTTGCCCCAGTCTAGAAGAATTGATTCTTGTTGATTGCACCAGCCTGATTGATGTTCATGAATCCATTGGAAACCTGGAGAGACTCGTGTACTTGAATATGAAGGATTGCAAGAATCTTAGGATGCTTCCGAAGAACGTGTGTTTTCTTAAATCACTTGAAACACTCATTTTATCTGGTTGCTCAAATCTTGATGAGTTTCCAGTGGAGATGATGAAGAAGATGGAGTCTCTAAAAGTTCTTGAAATAGATAAAATTCCAATAAGAGAATTATGGCCAGAAAGAAGTTCAACTATCTTGAGTTCTTTTCCATGCTCTTTAGTAGAGTTGAATCTTAGTAGGTGCAATCTTTCTGAAGATGCCTTTCCTACGGATTTAAGTAATCTATCCTCAATACGAAGACTATATCTAGATAAGAATCCAATTTGTAGCCTACCAGTTTTCATCAAAGGTTTGAGGAGGCTCGATTCTCTCTCTTTCCAGAGTTGTACGAGGCTCAAATCGCTTGTGGGGTTACCGAGAATACACAAAAATATGAATGTATCCTTTTGCAAATTATTAAAAAAAGTAACATATCAATCGTTATGGTTCCAAGAAGAGGCCTTTGTGGAAGACAACTACAACATGGTTGAGTGGGAGTACTATTTCAAATTAGAGCTTATTGATAGAGTTGATGTGGAAATGATCAAACTTTTGGGCTTGTGCAACTTGGAATCCATGCCTGCAATACGAATGAACAGCGATCTATTCTCATGTTCAAAAGTGAGTCCTGTCCAGGGACTGTATCAATTTGGTATATTCAGCACATTTTTTGTTGGGAAGGAGGTTCCAGGCCGGTTCAGCTATAAAAGTACCAAGTCCTCCATATCTTTTAGTGTCCCTTTACTCCCTTCTAGTCATAGAATCCGAGGCTTGAACATCTTTGCTACCTATGCAAACGAGGAGGATTCTGATAATGATGATTTTCCAATAATAATCAAAGTGAGTAACAAGAGCAAGGGTTTGAAGTGGATCTATTTCCCATTATTCTATGGTATCCCAGGTGACGGAGAAGATATGATATGGTTGAGCCATTGGAAGATGGAGAACGAAACAATATTACAATGTGGAGATCAAGTGGTGGTTTCAGTAATGGCCGGACCAGAGAAGTTGTTTCGTATAAAGGAGTTTGGTGTCGAGCTTATGCAAGAGCACCAGAATAATATGATGATAAGTACTCAACATAACACCAAATCAGATCCTAATTATCCAAATGTCTTCGGTGGAGATTTGTCCATGTGGGAGCATATACCAGGAATATACTTCCTTGGTTTCGATGACAAAATTGTTGAAGTGAAATGGTTTAATCACCTCATCATGGACGTTGATGAAGGAGACACAGAGAAAGAAGGGCAGGAGGATGAACCTGATTACACAGTTTTAAGGACAAGGGCTGCCAGTAACAACTGTGGCCTCAGAGGCTGGAAGGTGCTCGTCACAGCTGCCGGATTATTTTTCACGCTTGCTCTTGTAGTTCGGTCCTCCATCTCCCAGAAAGAGAAGCGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1015

Amino Acids

116.45

Weight (kDa)

6.62

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 42 - 212 2.6e-21 NB-ARC domain
AAA_22 PF13401 59 - 161 1.5e-07 AAA domain
WHD_ROQ1 PF23282 281 - 352 2.1e-17 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 521
Acc16I TGCGCA 1 cut(s) 1235
AccB1I GGYRCC 1 cut(s) 1086
AccI GTMKAC 1 cut(s) 178
AccII CGCG 1 cut(s) 1050
AclWI GGATC 7 cut(s) 169, 645, 1012, 1055, 1438, 2513, 2726
AcoI YGGCCR 2 cut(s) 1769, 2628
AcsI RAATTY 2 cut(s) 1424, 1749
AcuI CTGAAG 1 cut(s) 1869
AcyI GRCGYC 1 cut(s) 1087
AfaI GTAC 9 cut(s) 477, 681, 1129, 1266, 1586, 1984, 2135, 2347, 2710
AfiI CCNNNNNNNGG 7 cut(s) 650, 850, 1027, 2092, 2270, 2540, 2851
AflIII ACRYGT 2 cut(s) 229, 1632
AjnI CCWGG 7 cut(s) 633, 1063, 1569, 2268, 2323, 2533, 2785
AjuI GAANNNNNNNTTGG 2 cut(s) 749, 781
Alw21I GWGCWC 3 cut(s) 573, 2688, 2967
Alw26I GTCTC 5 cut(s) 1193, 1396, 1570, 1728, 2862
Alw44I GTGCAC 1 cut(s) 569
AlwI GGATC 7 cut(s) 169, 645, 1012, 1055, 1438, 2513, 2726
AlwNI CAGNNNCTG 2 cut(s) 2275, 2955
AoxI GGCC 5 cut(s) 1769, 2094, 2326, 2628, 2944
ApaLI GTGCAC 1 cut(s) 569
ApeKI GCWGC 2 cut(s) 2927, 2975
ApoI RAATTY 2 cut(s) 1424, 1749
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
Asp700I GAANNNNTTC 2 cut(s) 1041, 1518
AspLEI GCGC 2 cut(s) 1089, 1236
AspS9I GGNCC 4 cut(s) 451, 460, 2633, 3012
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 4 cut(s) 806, 988, 2549, 2834
AsuII TTCGAA 1 cut(s) 1104
AvaII GGWCC 4 cut(s) 451, 460, 2633, 3012
BaeGI GKGCMC 1 cut(s) 573
BalI TGGCCA 1 cut(s) 1771
BanI GGYRCC 1 cut(s) 1086
BanII GRGCYC 1 cut(s) 725
BarI GAAGNNNNNNTAC 2 cut(s) 354, 386
BauI CACGAG 1 cut(s) 1580
BbsI GAAGAC 4 cut(s) 218, 1900, 2112, 2743
Bbv12I GWGCWC 3 cut(s) 573, 2688, 2967
BbvCI CCTCAGC 1 cut(s) 1454
BbvI GCAGC 2 cut(s) 2914, 2962
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 7 cut(s) 635, 1065, 1571, 2270, 2325, 2535, 2787
BciVI GTATCC 2 cut(s) 2047, 2540
BclI TGATCA 1 cut(s) 2178
BcnI CCSGG 1 cut(s) 851
BcoDI GTCTC 5 cut(s) 1193, 1396, 1570, 1728, 2862
BfaI CTAG 6 cut(s) 420, 479, 528, 1508, 1904, 2388
BfmI CTRYAG 1 cut(s) 1296
BfoI RGCGCY 1 cut(s) 1090
BfuI GTATCC 2 cut(s) 2047, 2540
BglI GCCNNNNNGGC 1 cut(s) 2952
BisI GCNGC 2 cut(s) 2928, 2976
BlsI GCNGC 2 cut(s) 2929, 2977
BmcAI AGTACT 3 cut(s) 477, 2135, 2710
Bme1390I CCNGG 8 cut(s) 635, 851, 1065, 1571, 2270, 2325, 2535, 2787
Bme18I GGWCC 4 cut(s) 451, 460, 2633, 3012
BmgT120I GGNCC 4 cut(s) 451, 460, 2633, 3012
BmiI GGNNCC 3 cut(s) 1088, 2084, 2322
BmrFI CCNGG 8 cut(s) 635, 851, 1065, 1571, 2270, 2325, 2535, 2787
BmrI ACTGGG 1 cut(s) 1497
BmsI GCATC 7 cut(s) 460, 959, 980, 1177, 1221, 1608, 1843
BmuI ACTGGG 1 cut(s) 1497
BpiI GAAGAC 4 cut(s) 218, 1900, 2112, 2743
BpmI CTGGAG 1 cut(s) 1592
Bpu10I CCTNAGC 1 cut(s) 1454
Bpu14I TTCGAA 1 cut(s) 1104
BpuEI CTTGAG 2 cut(s) 111, 1813
BpuMI CCSGG 1 cut(s) 851
Bsa29I ATCGAT 1 cut(s) 493
BsaBI GATNNNNATC 1 cut(s) 1911
BsaHI GRCGYC 1 cut(s) 1087
BsaJI CCNNGG 5 cut(s) 1141, 2269, 2401, 2533, 2800
BsaXI ACNNNNNCTCC 2 cut(s) 2595, 2625
Bsc4I CCNNNNNNNGG 7 cut(s) 650, 850, 1027, 2092, 2270, 2540, 2851
Bse118I RCCGGY 1 cut(s) 2328
Bse1I ACTGG 7 cut(s) 186, 463, 1275, 1503, 1697, 1931, 2931
Bse3DI GCAATG 2 cut(s) 219, 867
Bse8I GATNNNNATC 1 cut(s) 1911
BseBI CCWGG 7 cut(s) 635, 1065, 1571, 2270, 2325, 2535, 2787
BseCI ATCGAT 1 cut(s) 493
BseDI CCNNGG 5 cut(s) 1141, 2269, 2401, 2533, 2800
BseGI GGATG 8 cut(s) 640, 929, 995, 1168, 1279, 1283, 1623, 2899
BseJI GATNNNNATC 1 cut(s) 1911
BseLI CCNNNNNNNGG 7 cut(s) 650, 850, 1027, 2092, 2270, 2540, 2851
BseMI GCAATG 2 cut(s) 219, 867
BseMII CTCAG 3 cut(s) 1188, 1468, 2962
BseNI ACTGG 7 cut(s) 186, 463, 1275, 1503, 1697, 1931, 2931
BseRI GAGGAG 2 cut(s) 1966, 2450
BseSI GKGCMC 1 cut(s) 573
BseXI GCAGC 2 cut(s) 2914, 2962
Bsh1236I CGCG 1 cut(s) 1050
BshFI GGCC 5 cut(s) 1771, 2096, 2328, 2630, 2946
BshNI GGYRCC 1 cut(s) 1086
BshVI ATCGAT 1 cut(s) 493
BsiHKAI GWGCWC 3 cut(s) 573, 2688, 2967
BsiSI CCGG 4 cut(s) 850, 2329, 2631, 2979
BslFI GGGAC 3 cut(s) 402, 2286, 2357
BslI CCNNNNNNNGG 7 cut(s) 650, 850, 1027, 2092, 2270, 2540, 2851
BsmAI GTCTC 5 cut(s) 1193, 1396, 1570, 1728, 2862
BsmFI GGGAC 3 cut(s) 402, 2286, 2357
BsmI GAATGC 1 cut(s) 948
BsnI GGCC 5 cut(s) 1771, 2096, 2328, 2630, 2946
Bsp119I TTCGAA 1 cut(s) 1104
Bsp1286I GDGCHC 4 cut(s) 573, 725, 2688, 2967
Bsp1407I TGTACA 1 cut(s) 679
Bsp68I TCGCGA 1 cut(s) 1050
BspANI GGCC 5 cut(s) 1771, 2096, 2328, 2630, 2946
BspCNI CTCAG 3 cut(s) 1189, 1467, 2961
BspDI ATCGAT 1 cut(s) 493
BspFNI CGCG 1 cut(s) 1050
BspHI TCATGA 2 cut(s) 801, 1552
BspLI GGNNCC 3 cut(s) 1088, 2084, 2322
BspPI GGATC 7 cut(s) 169, 645, 1012, 1055, 1438, 2513, 2726
BspQI GCTCTTC 1 cut(s) 730
BspT104I TTCGAA 1 cut(s) 1104
BspT107I GGYRCC 1 cut(s) 1086
BsrDI GCAATG 2 cut(s) 219, 867
BsrFI RCCGGY 1 cut(s) 2328
BsrGI TGTACA 1 cut(s) 679
BsrI ACTGG 7 cut(s) 186, 463, 1275, 1503, 1697, 1931, 2931
BssAI RCCGGY 1 cut(s) 2328
BssECI CCNNGG 5 cut(s) 1141, 2269, 2401, 2533, 2800
BssNAI GTATAC 1 cut(s) 179
BssNI GRCGYC 1 cut(s) 1087
BssSI CACGAG 1 cut(s) 1580
BssT1I CCWWGG 2 cut(s) 1141, 2800
Bst1107I GTATAC 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 1580
Bst2UI CCWGG 7 cut(s) 635, 1065, 1571, 2270, 2325, 2535, 2787
Bst4CI ACNGT 8 cut(s) 596, 830, 1181, 1204, 1394, 2277, 2911, 2942
Bst6I CTCTTC 4 cut(s) 418, 730, 1027, 2085
BstACI GRCGYC 1 cut(s) 1087
BstAUI TGTACA 1 cut(s) 679
BstBI TTCGAA 1 cut(s) 1104
BstC8I GCNNGC 3 cut(s) 624, 2217, 2997
BstDEI CTNAG 8 cut(s) 392, 647, 1197, 1256, 1454, 1613, 1829, 2948
BstEII GGTNACC 1 cut(s) 2007
BstF5I GGATG 8 cut(s) 640, 929, 995, 1168, 1279, 1283, 1623, 2899
BstFNI CGCG 1 cut(s) 1050
BstH2I RGCGCY 1 cut(s) 1090
BstHHI GCGC 2 cut(s) 1089, 1236
BstMAI GTCTC 5 cut(s) 1193, 1396, 1570, 1728, 2862
BstMWI GCNNNNNNNGC 4 cut(s) 1996, 2334, 2426, 2952
BstNI CCWGG 7 cut(s) 635, 1065, 1571, 2270, 2325, 2535, 2787
BstNSI RCATGY 3 cut(s) 199, 233, 626
BstPI GGTNACC 1 cut(s) 2007
BstSCI CCNGG 8 cut(s) 633, 849, 1063, 1569, 2268, 2323, 2533, 2785
BstSFI CTRYAG 1 cut(s) 1296
BstSLI GKGCMC 1 cut(s) 573
BstUI CGCG 1 cut(s) 1050
BstV1I GCAGC 2 cut(s) 2914, 2962
BstV2I GAAGAC 4 cut(s) 218, 1900, 2112, 2743
BstX2I RGATCY 4 cut(s) 1060, 1443, 2505, 2731
BstYI RGATCY 4 cut(s) 1060, 1443, 2505, 2731
BstZ17I GTATAC 1 cut(s) 179
Bsu15I ATCGAT 1 cut(s) 493
BsuI GTATCC 2 cut(s) 2047, 2540
BsuRI GGCC 5 cut(s) 1771, 2096, 2328, 2630, 2946
BsuTUI ATCGAT 1 cut(s) 493
BtsCI GGATG 8 cut(s) 640, 929, 995, 1168, 1279, 1283, 1623, 2899
BtsIMutI CAGTG 3 cut(s) 601, 1473, 1704
BtuMI TCGCGA 1 cut(s) 1050
Cac8I GCNNGC 3 cut(s) 624, 2217, 2997
CaiI CAGNNNCTG 2 cut(s) 2275, 2955
CciI TCATGA 2 cut(s) 801, 1552
CfoI GCGC 2 cut(s) 1089, 1236
Cfr10I RCCGGY 1 cut(s) 2328
Cfr13I GGNCC 4 cut(s) 451, 460, 2633, 3012
ClaI ATCGAT 1 cut(s) 493
Csp6I GTAC 9 cut(s) 476, 680, 1128, 1265, 1585, 1983, 2134, 2346, 2709
CspCI CAANNNNNGTGG 2 cut(s) 854, 889
CviQI GTAC 9 cut(s) 476, 680, 1128, 1265, 1585, 1983, 2134, 2346, 2709
DdeI CTNAG 8 cut(s) 392, 647, 1197, 1256, 1454, 1613, 1829, 2948
DinI GGCGCC 1 cut(s) 1088
EaeI YGGCCR 2 cut(s) 1769, 2628
Eam1104I CTCTTC 4 cut(s) 418, 730, 1027, 2085
EarI CTCTTC 4 cut(s) 418, 730, 1027, 2085
Eco130I CCWWGG 2 cut(s) 1141, 2800
Eco147I AGGCCT 1 cut(s) 2096
Eco24I GRGCYC 1 cut(s) 725
Eco47I GGWCC 4 cut(s) 451, 460, 2633, 3012
Eco57I CTGAAG 1 cut(s) 1869
Eco91I GGTNACC 1 cut(s) 2007
EcoO65I GGTNACC 1 cut(s) 2007
EcoRII CCWGG 7 cut(s) 633, 1063, 1569, 2268, 2323, 2533, 2785
EcoT14I CCWWGG 2 cut(s) 1141, 2800
EcoT22I ATGCAT 3 cut(s) 1011, 1170, 1222
EcoT38I GRGCYC 1 cut(s) 725
EgeI GGCGCC 1 cut(s) 1088
EheI GGCGCC 1 cut(s) 1088
ErhI CCWWGG 2 cut(s) 1141, 2800
FaqI GGGAC 3 cut(s) 402, 2286, 2357
FauNDI CATATG 1 cut(s) 456
FbaI TGATCA 1 cut(s) 2178
FblI GTMKAC 1 cut(s) 178
Fnu4HI GCNGC 2 cut(s) 2928, 2976
FokI GGATG 8 cut(s) 627, 936, 1002, 1155, 1286, 1290, 1630, 2906
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 2 cut(s) 2928, 2976
FspBI CTAG 6 cut(s) 420, 479, 528, 1508, 1904, 2388
FspI TGCGCA 1 cut(s) 1235
GlaI GCGC 2 cut(s) 1088, 1235
GluI GCNGC 2 cut(s) 2928, 2976
GsuI CTGGAG 1 cut(s) 1592
HaeII RGCGCY 1 cut(s) 1090
HaeIII GGCC 5 cut(s) 1771, 2096, 2328, 2630, 2946
HapII CCGG 4 cut(s) 850, 2329, 2631, 2979
HhaI GCGC 2 cut(s) 1089, 1236
Hin1I GRCGYC 1 cut(s) 1087
Hin6I GCGC 2 cut(s) 1087, 1234
HinP1I GCGC 2 cut(s) 1087, 1234
HindIII AAGCTT 1 cut(s) 253
HpaII CCGG 4 cut(s) 850, 2329, 2631, 2979
HphI GGTGA 4 cut(s) 806, 988, 2549, 2834
Hpy166II GTNNAC 7 cut(s) 179, 235, 451, 571, 682, 833, 1585
Hpy188I TCNGA 9 cut(s) 287, 670, 1057, 1626, 1849, 2402, 2446, 2731, 2951
Hpy8I GTNNAC 7 cut(s) 179, 235, 451, 571, 682, 833, 1585
Hpy99I CGWCG 2 cut(s) 695, 1050
HpyAV CCTTC 7 cut(s) 882, 1591, 2308, 2394, 2858, 2876, 2953
HpyCH4III ACNGT 8 cut(s) 596, 830, 1181, 1204, 1394, 2277, 2911, 2942
HpyCH4IV ACGT 4 cut(s) 999, 1072, 1632, 2856
HpyF10VI GCNNNNNNNGC 4 cut(s) 1996, 2334, 2426, 2952
HpyF3I CTNAG 8 cut(s) 392, 647, 1197, 1256, 1454, 1613, 1829, 2948
HpySE526I ACGT 4 cut(s) 999, 1072, 1632, 2856
Hsp92I GRCGYC 1 cut(s) 1087
HspAI GCGC 2 cut(s) 1087, 1234
KasI GGCGCC 1 cut(s) 1086
Ksp22I TGATCA 1 cut(s) 2178
LguI GCTCTTC 1 cut(s) 730
LmnI GCTCC 1 cut(s) 2776
Lsp1109I GCAGC 2 cut(s) 2914, 2962
LweI GCATC 7 cut(s) 460, 959, 980, 1177, 1221, 1608, 1843
MaeI CTAG 6 cut(s) 420, 479, 528, 1508, 1904, 2388
MaeII ACGT 4 cut(s) 999, 1072, 1632, 2856
MfeI CAATTG 2 cut(s) 939, 981
MflI RGATCY 4 cut(s) 1060, 1443, 2505, 2731
MhlI GDGCHC 4 cut(s) 573, 725, 2688, 2967
MlsI TGGCCA 1 cut(s) 1771
MluNI TGGCCA 1 cut(s) 1771
Mly113I GGCGCC 1 cut(s) 1087
MlyI GAGTC 6 cut(s) 41, 126, 608, 1572, 1730, 2269
MmeI TCCRAC 1 cut(s) 559
Mox20I TGGCCA 1 cut(s) 1771
Mph1103I ATGCAT 3 cut(s) 1011, 1170, 1222
MroXI GAANNNNTTC 2 cut(s) 1041, 1518
MscI TGGCCA 1 cut(s) 1771
MseI TTAA 7 cut(s) 57, 1304, 1644, 1871, 2054, 2838, 2915
MslI CAYNNNNRTG 2 cut(s) 362, 1464
Msp20I TGGCCA 1 cut(s) 1771
MspA1I CMGCKG 1 cut(s) 2975
MspI CCGG 4 cut(s) 850, 2329, 2631, 2979
MspR9I CCNGG 8 cut(s) 635, 851, 1065, 1571, 2270, 2325, 2535, 2787
MunI CAATTG 2 cut(s) 939, 981
Mva1269I GAATGC 1 cut(s) 948
MvaI CCWGG 7 cut(s) 635, 1065, 1571, 2270, 2325, 2535, 2787
MvnI CGCG 1 cut(s) 1050
MwoI GCNNNNNNNGC 4 cut(s) 1996, 2334, 2426, 2952
NarI GGCGCC 1 cut(s) 1087
NciI CCSGG 1 cut(s) 851
NdeI CATATG 1 cut(s) 456
NlaIV GGNNCC 3 cut(s) 1088, 2084, 2322
NmuCI GTSAC 4 cut(s) 976, 1022, 2537, 2968
NruI TCGCGA 1 cut(s) 1050
NsbI TGCGCA 1 cut(s) 1235
NsiI ATGCAT 3 cut(s) 1011, 1170, 1222
NspI RCATGY 3 cut(s) 199, 233, 626
NspV TTCGAA 1 cut(s) 1104
PaeI GCATGC 1 cut(s) 626
PagI TCATGA 2 cut(s) 801, 1552
PceI AGGCCT 1 cut(s) 2096
PciI ACATGT 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 730
PctI GAATGC 1 cut(s) 948
PdmI GAANNNNTTC 2 cut(s) 1041, 1518
PfoI TCCNGGA 1 cut(s) 1063
PkrI GCNGC 2 cut(s) 2929, 2977
PleI GAGTC 6 cut(s) 40, 125, 607, 1572, 1729, 2268
PluTI GGCGCC 1 cut(s) 1090
PpsI GAGTC 6 cut(s) 40, 125, 607, 1572, 1729, 2268
PscI ACATGT 1 cut(s) 229
PsiI TTATAA 1 cut(s) 521
Psp6I CCWGG 7 cut(s) 633, 1063, 1569, 2268, 2323, 2533, 2785
PspEI GGTNACC 1 cut(s) 2007
PspGI CCWGG 7 cut(s) 633, 1063, 1569, 2268, 2323, 2533, 2785
PspN4I GGNNCC 3 cut(s) 1088, 2084, 2322
PspPI GGNCC 4 cut(s) 451, 460, 2633, 3012
PstNI CAGNNNCTG 2 cut(s) 2275, 2955
PsuI RGATCY 4 cut(s) 1060, 1443, 2505, 2731
PvuII CAGCTG 1 cut(s) 2975
RruI TCGCGA 1 cut(s) 1050
RsaI GTAC 9 cut(s) 477, 681, 1129, 1266, 1586, 1984, 2135, 2347, 2710
RsaNI GTAC 9 cut(s) 476, 680, 1128, 1265, 1585, 1983, 2134, 2346, 2709
RseI CAYNNNNRTG 2 cut(s) 362, 1464
SapI GCTCTTC 1 cut(s) 730
SaqAI TTAA 7 cut(s) 57, 1304, 1644, 1871, 2054, 2838, 2915
SatI GCNGC 2 cut(s) 2928, 2976
Sau96I GGNCC 4 cut(s) 451, 460, 2633, 3012
ScaI AGTACT 3 cut(s) 477, 2135, 2710
SchI GAGTC 6 cut(s) 41, 126, 608, 1572, 1730, 2269
ScrFI CCNGG 8 cut(s) 635, 851, 1065, 1571, 2270, 2325, 2535, 2787
SduI GDGCHC 4 cut(s) 573, 725, 2688, 2967
SfaNI GCATC 7 cut(s) 460, 959, 980, 1177, 1221, 1608, 1843
SfcI CTRYAG 1 cut(s) 1296
SfoI GGCGCC 1 cut(s) 1088
SfuI TTCGAA 1 cut(s) 1104
SinI GGWCC 4 cut(s) 451, 460, 2633, 3012
SmiMI CAYNNNNRTG 2 cut(s) 362, 1464
SmlI CTYRAG 2 cut(s) 126, 1792
SmoI CTYRAG 2 cut(s) 126, 1792
SphI GCATGC 1 cut(s) 626
SseBI AGGCCT 1 cut(s) 2096
SspDI GGCGCC 1 cut(s) 1086
SspI AATATT 3 cut(s) 665, 915, 2592
SspMI CTAG 6 cut(s) 420, 479, 528, 1508, 1904, 2388
StuI AGGCCT 1 cut(s) 2096
StyD4I CCNGG 8 cut(s) 633, 849, 1063, 1569, 2268, 2323, 2533, 2785
StyI CCWWGG 2 cut(s) 1141, 2800
TaaI ACNGT 8 cut(s) 596, 830, 1181, 1204, 1394, 2277, 2911, 2942
TaiI ACGT 4 cut(s) 1002, 1075, 1635, 2859
TaqI TCGA 7 cut(s) 45, 493, 918, 1104, 1959, 2670, 2808
TaqII GACCGA 1 cut(s) 3000
TatI WGTACW 5 cut(s) 475, 679, 1584, 2133, 2708
Tru1I TTAA 7 cut(s) 57, 1304, 1644, 1871, 2054, 2838, 2915
Tru9I TTAA 7 cut(s) 57, 1304, 1644, 1871, 2054, 2838, 2915
TscAI CASTG 3 cut(s) 601, 1480, 1704
TseFI GTSAC 4 cut(s) 976, 1022, 2537, 2968
TseI GCWGC 2 cut(s) 2927, 2975
Tsp45I GTSAC 4 cut(s) 976, 1022, 2537, 2968
TspGWI ACGGA 4 cut(s) 708, 1010, 1880, 2556
TspRI CASTG 3 cut(s) 601, 1480, 1704
VneI GTGCAC 1 cut(s) 569
VpaK11BI GGWCC 4 cut(s) 451, 460, 2633, 3012
XapI RAATTY 2 cut(s) 1424, 1749
XbaI TCTAGA 3 cut(s) 419, 1507, 1903
XceI RCATGY 3 cut(s) 199, 233, 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 1567
XmiI GTMKAC 1 cut(s) 178
XmnI GAANNNNTTC 2 cut(s) 1041, 1518
XspI CTAG 6 cut(s) 420, 479, 528, 1508, 1904, 2388
ZrmI AGTACT 3 cut(s) 477, 2135, 2710
Zsp2I ATGCAT 3 cut(s) 1011, 1170, 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.