Rroxscaffold_4G00307000

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000036
Physical Location & Seq
Reverse (-)
28012618 .. 28018304
5687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00307000.1

Sequence Viewer

Length: 3615 bp
ATGGATGTTGTTAGCTCATCATCATCAGCTCAACAAGCCTCGTCTTCCGGTCAATTCACATATCAAGTGTCCTTGAGTTTCAGAGGCAGAAACACTCGCAAGACTTTCACCGATCATCTCTACACAGCCCTGACCAATGCAGGAATCTGCACTTTCCGAGACGACGACGAGCTCCCGGCCGGAGAAGCCATCAAGCCAGAACTAGAGAGAGCCATCCAACACTCGCGATGTTCTGTTATCGTGTTTTCAAAAGACTACGCGTCGTCCGGATGGTGCCTGGACGAGCTTGTGACGATCCTTCGATGCAAGAAGAGGTCCGGTCAGTTCGTTTTACCGATCTACTACGACGTCAGCCCATCCCAGGTGAGGAAGCGGACGGGGAGTCTTGCAAAGGCGTTCATTACACACCAGAAGAATCAAACAGCGGAAAAGCTGATTCTGTGGAAGGAAGCACTTACAGAAGTTGCGGCTCTAGCAGGCATGGTGTTAAAGGATCAAGTTGACGGGCACGAGTCCAAATTTATTGAAAATATTGTTAAGGTGATAGAAGGCAAGTTATGTCGAGTTCCCTTATGTGTTGCCCCTTACCTGGTCGGAATTGATTCTCGAGTAAAAAGCATCAATTTATGGTTACAGGATGGATCATCCAGTACTGGTATATTAGGAATTTGTGGAATTGGTGGTATTGGAAAGACAACTATTGCACAAATTGTTTATAATTCAAATTTTGAGAGATTTGAAAGAAGCTGCTTTCTTGAAAATATCAGAGAGATTTTCGAACGGCCAAGTGGTTTTGTTCAAGTACAGAAACAGCTTCTTTCGGATATTTTGAATGGGAGAAAAGTGAATATAAGCAGCCCTAGCGAGGGAATGAGTAAAATAAAAGATGCCTTAAGCTCTAAAAAAGTTCTTCTTGTTCTCGATGATGTGGATCACAAGGACCTAGTTGATGCAATACTTGAGATGAAAGGCTGCTTTTGTTCTGAAAGTAAAATTATAATAACAACAAGGAATGCAGGATTGCTCAAGGGTCATCGAGATATTAAGGTGCATAATGTCGAAACTTTGAACCATACTGAATCATTGGACCTCTTCAGCAGGCATGCTTTTGGACACAACCACCCTATTGAAAGTTATGTGAAACTTTCAGACAGGGTAGTGCACCACTGTGAAGGACTTCCATTAGCCCTTCAAACATTGGGTTCTTCTCTCTCAGGGAAGACTATAGATGTATGGGAAAGTACATTAAAGAAATCCAAAACTATTCCAAACAGTGTAGTGATGAGTAAACTGAGGATGAGCTTCAACTCTTTACAAGATGACCATGATCAGAGTTTATTCCTCCATATTGCATGTTTTTTTATCGGAAAGGACAAGGATATCATTGTCAAAATTTTAGATGCATGTGATTTCTGCACAACCGTTGGCATTCAAAATCTCATTGATAGATATTTGGTTAGAATTGAAGAATGTAATAAGGTGAATATGCATCAAATGATTTGTGACATGGGAAGAGAAATGGTTCGTCTAGAATCAAAAGATCCTAAGAAACGTAGTAGGCTATGGCATCACAAGGACTCTCTCAATGCATTAAGAGAAAAGACTGGTTCTAATGCAATTGAAGGCCTTGTCCTGAACATGCCTGTGAACCCTGCACATGGTCCTTCAAGAAATTCAGATATGGTAGTCTTGGATACCAATGCATTTTCAATGATGAACAATCTCAGATTCCTTCATCTTGGTCATGTACAACTCAATGGAAGTTATGAAAAATTTCCTAAAGGATTGAGATGGTTGTGTTGGCGCGAATGTCCTTTGAATTTAATACCGAGTGATTTTCCTATGGAGATGATGGTTGTTATTGAAATGTCCTGTAGTAGATTGAGACAAGTGTGGAAGGGAGCAAAGTGTCTTCCATCATTGAAGATCTTTGATCTCAGCCATTCCTCAAACCTTACTGAAACTGGTGACTTCTCATTGGTCCAGAATCTGGAGGGACTTACACTTGAACATTGTATAAGCTTAGTTGATGTCCATGAATCCATAGGAAAGCTAGAGAAACTTGTTTACTTGAATTTGAAAGATTGCATAAATATCATGAAGCTTCCAGAGAACATTCATATGTTAAAATCACTTGAAACACTTATTATATCTGGTTGTTCAAATCTTAGTGAGTTTCCAATGGAGATTAGAAACATGGAAACTCTTAAAGTGTTTCAAGCATTTGGAGTTCCAATAGATCAGCTAATCACTATCCCTGGGCAGGTAAATTCACGGCCAAGATTAAGTATAGATGCTTTTTGGGGTTCCTTTTCTGGCTCTATTGTTGAGTTAAATCTTGGAGGCTGCAATCTTTCTGATGATGCTTTTCCTAGTGATTTTGGTAACTTATCTTCATTGGAGAGTTTGGATCTCAGCTACAATAACTTTTGTACCTTGCCAGATTCAATCAGAGGCCTTTGTAAACTCAAGACGCTTAATCTTCAATCCTGTAGAAGTCTCATGTCAATTACAGGTCTACCACGAGTAAGAGATCTACTGAGCACAGACCATTGTGTATCCTTGGAAAAAATAACATTTCAATCACTTTCATGCATACCGAAGAGAGTATCCATATATGGTGACTCCAAACTAGTTGAGATTCAGCACTGGTACAAGCTAAAACCCATTGGAACAGTTGATGTAGAAATGATCAGGCTTTTGGGTTTGTCCAACTTGGAATCCATGGAACCCATTCTGATGCACACCGCAGAAGCAAACTCCCTGTCGGAAGGGGAATCCATGGAACCCATTTGCATCCAGGGACTACATGAATATGGTATCTTCAGCACATTCTTTCCAGGAAACGGAAATGAGGTTCCAGGACAGTTCAGCCATACAAGTAAAGGGTGTTCCTCAATATCTTTTCCTGTGCCTATACTTCTTAATCACAATATCCGAGGCTTCAACATATATTCTGTGTATGCATGGATGACAGATGTTACTATATTGCCACCATTAATCATTGAAGTCAATAATAAGAGCACGGGTCTGAAGTGGATCTATGCGCCGTCATGTTATGGCATTCCAAGTGATGGAAAAGACATGATATGGTTAAGCCATTGGAGTTTGGGGAACCAATTCAAAGGTGGCGATGTAGTCACTGTCTCAGTATTTACGCCACATAACTGGCTTCAGGTAAAACAGTTCGGTGTCCAAGTGGTGCATGAGGAAGAAGAGAAGAAGAGTAAGCTACAGTACAGCAGCAGTAGTAGAGATCCTAGTTATGATGATCCCGATGCTTGTGGTGTTAATGAGGTGATTGGTAATAGTGGTGGAGTTTTGTCAGAGTTATATCAGGTCAGGCCAGGGACATACTTCCTCTGCGGTGGACCCTTTTTAGAAAATTATAGATACTCATATAACAGATGGAAAAAGGATCGTTGCCTCAGTGGCATCATAGGAGAGTATGATGAAGAAACAGACAAAGAAGAAAGGAAACAGGGGGATAGGACACTAGCAGCAACAGAAGAAGCTGAAGCAGAAAGAGGCGGCAGCAATGAATTCTTTGGTTGCAAGAACCTTGTTATCCATGTGCTTATGTCATTGGTTGAGATTTGTCAAAATATCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1204

Amino Acids

134.78

Weight (kDa)

6.28

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 185 1.1e-47 TIR domain
TIR_2 PF13676 24 - 135 2.7e-14 TIR domain
NB-ARC PF00931 203 - 371 3.4e-20 NB-ARC domain
WHD_ROQ1 PF23282 441 - 511 8.4e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 661 - 739 5.4e-08 Leucine-rich repeat region
LRR_8 PF13855 777 - 832 5.6e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 717, 998
AatII GACGTC 1 cut(s) 351
Acc36I ACCTGC 1 cut(s) 2254
AccB1I GGYRCC 1 cut(s) 273
AccB7I CCANNNNNTGG 2 cut(s) 1990, 3074
AccI GTMKAC 1 cut(s) 2518
AccII CGCG 3 cut(s) 226, 260, 1806
AccIII TCCGGA 1 cut(s) 266
AciI CCGC 6 cut(s) 373, 425, 467, 2748, 3366, 3531
AcoI YGGCCR 3 cut(s) 177, 782, 2276
AcuI CTGAAG 5 cut(s) 1078, 2809, 3053, 3158, 3537
AcyI GRCGYC 1 cut(s) 348
AfaI GTAC 7 cut(s) 652, 804, 1243, 1749, 2434, 2654, 3239
AflII CTTAAG 1 cut(s) 892
AflIII ACRYGT 1 cut(s) 258
AhdI GACNNNNNGTC 2 cut(s) 259, 381
AhlI ACTAGT 1 cut(s) 2632
AjnI CCWGG 8 cut(s) 276, 360, 588, 2257, 2799, 2839, 2860, 3346
AleI CACNNNNGTG 1 cut(s) 1167
AloI GAACNNNNNNTCC 2 cut(s) 2841, 2873
Alw21I GWGCWC 4 cut(s) 174, 1164, 2546, 3026
Alw26I GTCTC 4 cut(s) 153, 1879, 2504, 3151
Alw44I GTGCAC 1 cut(s) 1160
AlwNI CAGNNNCTG 1 cut(s) 1990
Ama87I CYCGRG 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 6 cut(s) 177, 782, 1624, 2276, 2455, 3344
ApaLI GTGCAC 1 cut(s) 1160
ApeKI GCWGC 7 cut(s) 747, 855, 972, 2346, 3243, 3500, 3534
ArsI GACNNNNNNTTYG 2 cut(s) 2750, 2782
AseI ATTAAT 1 cut(s) 2999
Asp700I GAANNNNTTC 6 cut(s) 601, 1176, 1521, 1773, 2733, 3119
AspLEI GCGC 2 cut(s) 1806, 3049
AspS9I GGNCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 7 cut(s) 100, 376, 553, 1492, 1979, 2633, 3310
AsuII TTCGAA 1 cut(s) 777
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
BaeGI GKGCMC 2 cut(s) 510, 1164
BaeI ACNNNNGTAYC 2 cut(s) 2416, 2449
BanI GGYRCC 1 cut(s) 273
BanII GRGCYC 1 cut(s) 174
BarI GAAGNNNNNNTAC 2 cut(s) 2377, 2409
BauI CACGAG 2 cut(s) 509, 2523
BbsI GAAGAC 3 cut(s) 36, 1226, 1904
Bbv12I GWGCWC 4 cut(s) 174, 1164, 2546, 3026
BbvI GCAGC 7 cut(s) 734, 867, 959, 2333, 3255, 3512, 3546
BceAI ACGGC 3 cut(s) 797, 2291, 3034
BciT130I CCWGG 8 cut(s) 278, 362, 590, 2259, 2801, 2841, 2862, 3348
BciVI GTATCC 3 cut(s) 1687, 2569, 2620
BclI TGATCA 2 cut(s) 1327, 2691
BcnI CCSGG 1 cut(s) 176
BcoDI GTCTC 4 cut(s) 153, 1879, 2504, 3151
BcuI ACTAGT 1 cut(s) 2632
BfmI CTRYAG 4 cut(s) 1224, 1873, 2491, 3233
BfrI CTTAAG 1 cut(s) 892
BfuAI ACCTGC 1 cut(s) 2254
BfuI GTATCC 3 cut(s) 1687, 2569, 2620
BglI GCCNNNNNGGC 1 cut(s) 3432
BglII AGATCT 2 cut(s) 1926, 2533
BisI GCNGC 9 cut(s) 468, 748, 856, 973, 2347, 3244, 3501, 3532, 3535
BlsI GCNGC 9 cut(s) 469, 749, 857, 974, 2348, 3245, 3502, 3533, 3536
BmcAI AGTACT 1 cut(s) 652
Bme1390I CCNGG 9 cut(s) 176, 278, 362, 590, 2259, 2801, 2841, 2862, 3348
Bme18I GGWCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
BmeRI GACNNNNNGTC 2 cut(s) 259, 381
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
BmiI GGNNCC 7 cut(s) 275, 2308, 2730, 2787, 2859, 3116, 3373
BmrFI CCNGG 9 cut(s) 176, 278, 362, 590, 2259, 2801, 2841, 2862, 3348
BpiI GAAGAC 3 cut(s) 36, 1226, 1904
BpmI CTGGAG 1 cut(s) 2012
Bpu14I TTCGAA 1 cut(s) 777
BpuEI CTTGAG 4 cut(s) 94, 980, 1010, 2453
BpuMI CCSGG 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 930
BsaHI GRCGYC 1 cut(s) 348
BsaJI CCNNGG 9 cut(s) 360, 2257, 2258, 2562, 2724, 2781, 2800, 2938, 3347
BsaWI WCCGGW 3 cut(s) 47, 266, 317
Bse1I ACTGG 6 cut(s) 648, 658, 1609, 1969, 2654, 3173
Bse3DI GCAATG 1 cut(s) 3544
Bse8I GATNNNNATC 1 cut(s) 930
BseAI TCCGGA 1 cut(s) 266
BseBI CCWGG 8 cut(s) 278, 362, 590, 2259, 2801, 2841, 2862, 3348
BseDI CCNNGG 9 cut(s) 360, 2257, 2258, 2562, 2724, 2781, 2800, 2938, 3347
BseGI GGATG 9 cut(s) 10, 213, 275, 356, 643, 644, 1302, 2796, 2976
BseJI GATNNNNATC 1 cut(s) 930
BseMI GCAATG 1 cut(s) 3544
BseMII CTCAG 8 cut(s) 1227, 1283, 1738, 1951, 2428, 2531, 3162, 3442
BseNI ACTGG 6 cut(s) 648, 658, 1609, 1969, 2654, 3173
BseSI GKGCMC 2 cut(s) 510, 1164
BseX3I CGGCCG 1 cut(s) 177
BseXI GCAGC 7 cut(s) 734, 867, 959, 2333, 3255, 3512, 3546
BsgI GTGCAG 3 cut(s) 133, 1399, 1638
Bsh1236I CGCG 3 cut(s) 226, 260, 1806
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 6 cut(s) 179, 784, 1626, 2278, 2457, 3346
BshNI GGYRCC 1 cut(s) 273
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 4 cut(s) 174, 1164, 2546, 3026
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 5 cut(s) 48, 176, 180, 267, 318
BslFI GGGAC 3 cut(s) 2010, 2817, 3364
BsmAI GTCTC 4 cut(s) 153, 1879, 2504, 3151
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 3 cut(s) 2010, 2817, 3364
BsmI GAATGC 3 cut(s) 1018, 1428, 3063
BsnI GGCC 6 cut(s) 179, 784, 1626, 2278, 2457, 3346
BsoBI CYCGRG 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 777
Bsp1286I GDGCHC 5 cut(s) 174, 510, 1164, 2546, 3026
Bsp13I TCCGGA 1 cut(s) 266
Bsp1407I TGTACA 1 cut(s) 1747
Bsp19I CCATGG 2 cut(s) 2724, 2781
Bsp68I TCGCGA 1 cut(s) 226
BspACI CCGC 6 cut(s) 373, 425, 467, 2748, 3366, 3531
BspANI GGCC 6 cut(s) 179, 784, 1626, 2278, 2457, 3346
BspCNI CTCAG 8 cut(s) 1226, 1284, 1737, 1950, 2427, 2532, 3161, 3441
BspEI TCCGGA 1 cut(s) 266
BspFNI CGCG 3 cut(s) 226, 260, 1806
BspHI TCATGA 1 cut(s) 2097
BspLI GGNNCC 7 cut(s) 275, 2308, 2730, 2787, 2859, 3116, 3373
BspMI ACCTGC 1 cut(s) 2254
BspT104I TTCGAA 1 cut(s) 777
BspT107I GGYRCC 1 cut(s) 273
BspTI CTTAAG 1 cut(s) 892
BsrDI GCAATG 1 cut(s) 3544
BsrGI TGTACA 1 cut(s) 1747
BsrI ACTGG 6 cut(s) 648, 658, 1609, 1969, 2654, 3173
BssECI CCNNGG 9 cut(s) 360, 2257, 2258, 2562, 2724, 2781, 2800, 2938, 3347
BssNI GRCGYC 1 cut(s) 348
BssSI CACGAG 2 cut(s) 509, 2523
BssT1I CCWWGG 3 cut(s) 2562, 2724, 2781
Bst2BI CACGAG 2 cut(s) 509, 2523
Bst2UI CCWGG 8 cut(s) 278, 362, 590, 2259, 2801, 2841, 2862, 3348
Bst4CI ACNGT 8 cut(s) 1169, 1274, 1423, 2677, 2868, 3145, 3186, 3237
Bst6I CTCTTC 6 cut(s) 305, 1097, 1507, 2597, 3210, 3218
BstACI GRCGYC 1 cut(s) 348
BstAFI CTTAAG 1 cut(s) 892
BstAUI TGTACA 1 cut(s) 1747
BstBI TTCGAA 1 cut(s) 777
BstC8I GCNNGC 3 cut(s) 478, 1100, 1104
BstDSI CCRYGG 2 cut(s) 2724, 2781
BstF5I GGATG 9 cut(s) 10, 213, 275, 356, 643, 644, 1302, 2796, 2976
BstFNI CGCG 3 cut(s) 226, 260, 1806
BstHHI GCGC 2 cut(s) 1806, 3049
BstMAI GTCTC 4 cut(s) 153, 1879, 2504, 3151
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 5 cut(s) 35, 185, 473, 861, 3432
BstNI CCWGG 8 cut(s) 278, 362, 590, 2259, 2801, 2841, 2862, 3348
BstNSI RCATGY 4 cut(s) 1106, 1356, 1407, 1642
BstSCI CCNGG 9 cut(s) 174, 276, 360, 588, 2257, 2799, 2839, 2860, 3346
BstSFI CTRYAG 4 cut(s) 1224, 1873, 2491, 3233
BstSLI GKGCMC 2 cut(s) 510, 1164
BstUI CGCG 3 cut(s) 226, 260, 1806
BstV1I GCAGC 7 cut(s) 734, 867, 959, 2333, 3255, 3512, 3546
BstV2I GAAGAC 3 cut(s) 36, 1226, 1904
BstX2I RGATCY 6 cut(s) 1540, 1926, 2410, 2533, 3039, 3256
BstXI CCANNNNNNTGG 1 cut(s) 3168
BstYI RGATCY 6 cut(s) 1540, 1926, 2410, 2533, 3039, 3256
BstZI CGGCCG 1 cut(s) 177
BsuI GTATCC 3 cut(s) 1687, 2569, 2620
BsuRI GGCC 6 cut(s) 179, 784, 1626, 2278, 2457, 3346
BtgI CCRYGG 2 cut(s) 2724, 2781
BtgZI GCGATG 2 cut(s) 241, 3147
BtsCI GGATG 9 cut(s) 10, 213, 275, 356, 643, 644, 1302, 2796, 2976
BtsIMutI CAGTG 5 cut(s) 1165, 1279, 2647, 3141, 3436
BtuMI TCGCGA 1 cut(s) 226
BveI ACCTGC 1 cut(s) 2254
Cac8I GCNNGC 3 cut(s) 478, 1100, 1104
CaiI CAGNNNCTG 1 cut(s) 1990
CciI TCATGA 1 cut(s) 2097
CfoI GCGC 2 cut(s) 1806, 3049
Cfr13I GGNCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
CseI GACGC 2 cut(s) 249, 2482
CsiI ACCWGGT 1 cut(s) 588
Csp6I GTAC 7 cut(s) 651, 803, 1242, 1748, 2433, 2653, 3238
CviQI GTAC 7 cut(s) 651, 803, 1242, 1748, 2433, 2653, 3238
DriI GACNNNNNGTC 2 cut(s) 259, 381
EaeI YGGCCR 3 cut(s) 177, 782, 2276
EagI CGGCCG 1 cut(s) 177
Eam1104I CTCTTC 6 cut(s) 305, 1097, 1507, 2597, 3210, 3218
Eam1105I GACNNNNNGTC 2 cut(s) 259, 381
EarI CTCTTC 6 cut(s) 305, 1097, 1507, 2597, 3210, 3218
Ecl136II GAGCTC 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 177
Eco130I CCWWGG 3 cut(s) 2562, 2724, 2781
Eco147I AGGCCT 2 cut(s) 1626, 2457
Eco24I GRGCYC 1 cut(s) 174
Eco32I GATATC 1 cut(s) 1381
Eco47I GGWCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
Eco52I CGGCCG 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 5 cut(s) 1078, 2809, 3053, 3158, 3537
Eco88I CYCGRG 1 cut(s) 606
EcoICRI GAGCTC 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 940
EcoRI GAATTC 1 cut(s) 3542
EcoRII CCWGG 8 cut(s) 276, 360, 588, 2257, 2799, 2839, 2860, 3346
EcoRV GATATC 1 cut(s) 1381
EcoT14I CCWWGG 3 cut(s) 2562, 2724, 2781
EcoT22I ATGCAT 6 cut(s) 1405, 1491, 1591, 1705, 2597, 2968
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 3 cut(s) 2562, 2724, 2781
Esp3I CGTCTC 1 cut(s) 153
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FaqI GGGAC 3 cut(s) 2010, 2817, 3364
FauNDI CATATG 1 cut(s) 2121
FbaI TGATCA 2 cut(s) 1327, 2691
FblI GTMKAC 1 cut(s) 2518
Fnu4HI GCNGC 9 cut(s) 468, 748, 856, 973, 2347, 3244, 3501, 3532, 3535
FokI GGATG 9 cut(s) 17, 200, 282, 343, 631, 650, 1309, 2783, 2983
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 9 cut(s) 468, 748, 856, 973, 2347, 3244, 3501, 3532, 3535
GlaI GCGC 2 cut(s) 1805, 3048
GluI GCNGC 9 cut(s) 468, 748, 856, 973, 2347, 3244, 3501, 3532, 3535
GsuI CTGGAG 1 cut(s) 2012
HaeIII GGCC 6 cut(s) 179, 784, 1626, 2278, 2457, 3346
HapII CCGG 5 cut(s) 48, 176, 180, 267, 318
HgaI GACGC 2 cut(s) 249, 2482
HhaI GCGC 2 cut(s) 1806, 3049
Hin1I GRCGYC 1 cut(s) 348
Hin6I GCGC 2 cut(s) 1804, 3047
HinP1I GCGC 2 cut(s) 1804, 3047
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 2 cut(s) 2020, 2102
HpaII CCGG 5 cut(s) 48, 176, 180, 267, 318
HphI GGTGA 7 cut(s) 100, 376, 553, 1492, 1979, 2633, 3310
Hpy166II GTNNAC 8 cut(s) 502, 1162, 1289, 1648, 2068, 2465, 2519, 3371
Hpy8I GTNNAC 8 cut(s) 502, 1162, 1289, 1648, 2068, 2465, 2519, 3371
Hpy99I CGWCG 4 cut(s) 167, 170, 265, 350
HpyCH4III ACNGT 8 cut(s) 1169, 1274, 1423, 2677, 2868, 3145, 3186, 3237
HpyCH4IV ACGT 2 cut(s) 348, 1552
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 185, 473, 861, 3432
HpySE526I ACGT 2 cut(s) 348, 1552
Hsp92I GRCGYC 1 cut(s) 348
HspAI GCGC 2 cut(s) 1804, 3047
Kpn2I TCCGGA 1 cut(s) 266
Ksp22I TGATCA 2 cut(s) 1327, 2691
LmnI GCTCC 2 cut(s) 177, 1901
Lsp1109I GCAGC 7 cut(s) 734, 867, 959, 2333, 3255, 3512, 3546
MabI ACCWGGT 1 cut(s) 588
MaeII ACGT 2 cut(s) 348, 1552
MaeIII GTNAC 8 cut(s) 289, 630, 1502, 1967, 2384, 2621, 2980, 3139
MfeI CAATTG 1 cut(s) 1617
MflI RGATCY 6 cut(s) 1540, 1926, 2410, 2533, 3039, 3256
MhlI GDGCHC 5 cut(s) 174, 510, 1164, 2546, 3026
MluI ACGCGT 1 cut(s) 258
MlyI GAGTC 4 cut(s) 391, 521, 1571, 2618
MmeI TCCRAC 4 cut(s) 241, 574, 2736, 2748
Mph1103I ATGCAT 6 cut(s) 1405, 1491, 1591, 1705, 2597, 2968
MroI TCCGGA 1 cut(s) 266
MroXI GAANNNNTTC 6 cut(s) 601, 1176, 1521, 1773, 2733, 3119
MslI CAYNNNNRTG 6 cut(s) 1167, 1643, 2120, 2590, 2738, 2814
MspA1I CMGCKG 1 cut(s) 425
MspCI CTTAAG 1 cut(s) 892
MspI CCGG 5 cut(s) 48, 176, 180, 267, 318
MspR9I CCNGG 9 cut(s) 176, 278, 362, 590, 2259, 2801, 2841, 2862, 3348
MunI CAATTG 1 cut(s) 1617
Mva1269I GAATGC 3 cut(s) 1018, 1428, 3063
MvaI CCWGG 8 cut(s) 278, 362, 590, 2259, 2801, 2841, 2862, 3348
MvnI CGCG 3 cut(s) 226, 260, 1806
MwoI GCNNNNNNNGC 5 cut(s) 35, 185, 473, 861, 3432
NciI CCSGG 1 cut(s) 176
NcoI CCATGG 2 cut(s) 2724, 2781
NdeI CATATG 1 cut(s) 2121
NlaIV GGNNCC 7 cut(s) 275, 2308, 2730, 2787, 2859, 3116, 3373
NmuCI GTSAC 5 cut(s) 289, 1502, 1967, 2621, 3139
NruI TCGCGA 1 cut(s) 226
NsiI ATGCAT 6 cut(s) 1405, 1491, 1591, 1705, 2597, 2968
NspI RCATGY 4 cut(s) 1106, 1356, 1407, 1642
NspV TTCGAA 1 cut(s) 777
OliI CACNNNNGTG 1 cut(s) 1167
PaeI GCATGC 1 cut(s) 1106
PaeR7I CTCGAG 1 cut(s) 606
PagI TCATGA 1 cut(s) 2097
PasI CCCWGGG 1 cut(s) 2258
PceI AGGCCT 2 cut(s) 1626, 2457
PctI GAATGC 3 cut(s) 1018, 1428, 3063
PdmI GAANNNNTTC 6 cut(s) 601, 1176, 1521, 1773, 2733, 3119
PflMI CCANNNNNTGG 2 cut(s) 1990, 3074
PfoI TCCNGGA 2 cut(s) 2839, 2860
PkrI GCNGC 9 cut(s) 469, 749, 857, 974, 2348, 3245, 3502, 3533, 3536
PleI GAGTC 4 cut(s) 390, 520, 1571, 2618
PpsI GAGTC 4 cut(s) 390, 520, 1571, 2618
PpuMI RGGWCCY 1 cut(s) 940
PshBI ATTAAT 1 cut(s) 2999
PsiI TTATAA 2 cut(s) 717, 998
Psp124BI GAGCTC 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 940
Psp6I CCWGG 8 cut(s) 276, 360, 588, 2257, 2799, 2839, 2860, 3346
PspGI CCWGG 8 cut(s) 276, 360, 588, 2257, 2799, 2839, 2860, 3346
PspN4I GGNNCC 7 cut(s) 275, 2308, 2730, 2787, 2859, 3116, 3373
PspPI GGNCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
PspPPI RGGWCCY 1 cut(s) 940
PsrI GAACNNNNNNTAC 2 cut(s) 2875, 2907
PstNI CAGNNNCTG 1 cut(s) 1990
PsuI RGATCY 6 cut(s) 1540, 1926, 2410, 2533, 3039, 3256
RruI TCGCGA 1 cut(s) 226
RsaI GTAC 7 cut(s) 652, 804, 1243, 1749, 2434, 2654, 3239
RsaNI GTAC 7 cut(s) 651, 803, 1242, 1748, 2433, 2653, 3238
RseI CAYNNNNRTG 6 cut(s) 1167, 1643, 2120, 2590, 2738, 2814
SacI GAGCTC 1 cut(s) 174
SatI GCNGC 9 cut(s) 468, 748, 856, 973, 2347, 3244, 3501, 3532, 3535
Sau96I GGNCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
ScaI AGTACT 1 cut(s) 652
SchI GAGTC 4 cut(s) 391, 521, 1571, 2618
ScrFI CCNGG 9 cut(s) 176, 278, 362, 590, 2259, 2801, 2841, 2862, 3348
SduI GDGCHC 5 cut(s) 174, 510, 1164, 2546, 3026
SexAI ACCWGGT 1 cut(s) 588
SfcI CTRYAG 4 cut(s) 1224, 1873, 2491, 3233
Sfr274I CTCGAG 1 cut(s) 606
SfuI TTCGAA 1 cut(s) 777
SinI GGWCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
SlaI CTCGAG 1 cut(s) 606
SmiMI CAYNNNNRTG 6 cut(s) 1167, 1643, 2120, 2590, 2738, 2814
SmlI CTYRAG 6 cut(s) 73, 606, 892, 959, 1025, 2468
SmoI CTYRAG 6 cut(s) 73, 606, 892, 959, 1025, 2468
SpeI ACTAGT 1 cut(s) 2632
SphI GCATGC 1 cut(s) 1106
SseBI AGGCCT 2 cut(s) 1626, 2457
SsiI CCGC 6 cut(s) 373, 425, 467, 2748, 3366, 3531
SspI AATATT 1 cut(s) 532
SstI GAGCTC 1 cut(s) 174
StuI AGGCCT 2 cut(s) 1626, 2457
StyD4I CCNGG 9 cut(s) 174, 276, 360, 588, 2257, 2799, 2839, 2860, 3346
StyI CCWWGG 3 cut(s) 2562, 2724, 2781
TaaI ACNGT 8 cut(s) 1169, 1274, 1423, 2677, 2868, 3145, 3186, 3237
TaiI ACGT 2 cut(s) 351, 1555
TaqI TCGA 7 cut(s) 301, 562, 607, 777, 921, 1036, 1059
TatI WGTACW 5 cut(s) 650, 802, 1241, 1747, 3237
TauI GCSGC 2 cut(s) 470, 3534
TscAI CASTG 5 cut(s) 1172, 1279, 2654, 3148, 3436
TseFI GTSAC 5 cut(s) 289, 1502, 1967, 2621, 3139
TseI GCWGC 7 cut(s) 747, 855, 972, 2346, 3243, 3500, 3534
Tsp45I GTSAC 5 cut(s) 289, 1502, 1967, 2621, 3139
TspGWI ACGGA 1 cut(s) 2862
TspRI CASTG 5 cut(s) 1172, 1279, 2654, 3148, 3436
Van91I CCANNNNNTGG 2 cut(s) 1990, 3074
Vha464I CTTAAG 1 cut(s) 892
VneI GTGCAC 1 cut(s) 1160
VpaK11BI GGWCC 6 cut(s) 315, 940, 1087, 1661, 1981, 3371
VspI ATTAAT 1 cut(s) 2999
XbaI TCTAGA 1 cut(s) 1528
XceI RCATGY 4 cut(s) 1106, 1356, 1407, 1642
XcmI CCANNNNNNNNNTGG 1 cut(s) 3125
XhoI CTCGAG 1 cut(s) 606
XmiI GTMKAC 1 cut(s) 2518
XmnI GAANNNNTTC 6 cut(s) 601, 1176, 1521, 1773, 2733, 3119
ZraI GACGTC 1 cut(s) 349
ZrmI AGTACT 1 cut(s) 652
Zsp2I ATGCAT 6 cut(s) 1405, 1491, 1591, 1705, 2597, 2968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.