Prupe.2G126100_v2.0.a1

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000036
Physical Location & Seq
Reverse (-)
18253334 .. 18260122
6789 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G126100.1

Sequence Viewer

Length: 3519 bp
ATGGCACTGGTGAGAGCAGCTCAGCAAACCTCCTCTTCTAATGCTCGTCGACATTGTCTTTATCACGTGTTCCTGAGTTTCAGAGGCGAAGACACTCGCAAGACTTTCACTGACCACCTCTACACAGCCTTGGTCAACGCCCGATTTCACACTTTCCGAGACGACGATGAGCTTGAGAGAGGAGAAGAGATTAAGCCCGAACTGGAGAAAGCAATCAAAAACTCTCGAAGTTCTGTTATTGTGTTTTCGAAAGACTATGCGTCGTCCAGATGGTGCCTCGACGAGCTTGTGGTGATCCTTGAACACAAGAGGACCTCAGATGACCATGTCGTTTTACCTGTTTTCTACGACGTGGATCCATCCCATGTAAGGAAGCAGACAGGAAGTCTTGCAAAGGCATTTGCTAGACACGAAAAAACTCAACCGCTGGAGAAGTTGAAGGAATGGAGGGATGCACTTGCAAAAGTTGCGGATCTGGCAGGGATGGTCTTGCAAAATCAAGCTCATGGGTACGAGGCAAAGTTTATCAAGAAAATAGTTAAGGTAATCGGAGAAAAACTGAGTCGCACACCCTTAAATGTTGACCGCAACATGATTGGAATGCAATATCGAGTGCAAAATATCAACTTATGGTTACAGCATGGATCAACTGATGATGTTGGCATACTTGTAATATATGGGATAAGTGGAATAGGGAAGACAACCATTGCAAAGCATGTTTATAACTCAAACTTCCAAAAATTTGAAGGAAGCAGCTTCCTTGAAAACATCAAAGAAATTTCACAGCAACCCAATGGCTTAGTTCAAATACAAACGCAACTTCTTTATGATATTTTGAATGGGACAGAAGTGAAAATACACGGTGTTAGTCAAGGAATAACTGAGATTGAAAAAGCCATAAGTTCTAAACGGGTTCTCCTTGTTCTCGATGATGTAGATCATGTGGACCAATTAAATGCAGTACATCTGATGAAAGATCGATTTTGTCCAGGAAGTAAAATCATCGTAACAACTAGGCATCGAGGATTGTTAGAGGCTCATCAATTTATTACTGAGGTGCATGCTGTTAAAACTTTGGATCATATAGAATCTTTGGAGCTCTTAAGTTGGCATGCTTTTGGCCAGGACCATCCGCTTGAAGATTACACAGAGTATTCAAAAAAGCTAGTGGACCATTGCGGAGGACTTCCGTTAGCTCTTAAAGTTTTAGGGTCTTCTCTATTTGGGAAAAGTATATATATATGGAAAAGTGCACTGAAGAAGTTGGAAGATATTCCAAATGGTGAAATAATAAGGAAACTAAGAGTAAGCTACGACTCTTTGCAAGATGACCATGACCAAAAATTATTCCTCCACATTGCTTGTTTCTTTATTGGAAAGGACAAAGATTGCATTGTTACAATACTAGATGGATGTGATTTCCATACACTTGTCACTATTGAATATCTCATCCATAGGTGTTTGGTAACAATCGATGAACATGACAAGGTGCAGATGCATGACTTGATTCGTGGAATGGGAAGAGAAATTGTTCGTCTAGAATCAGAGAAGCTTTGCAAGCGTAGTAGAGTATGGCGTCATCGAGATTCCTTTGAAATCCTGACAAAAAAGAATGGTACAAGAAAAATTGAAGGTCTTGTCCTGGACATGCATATGCTCCCCACACAGAGTCTCATAAACTCAAATGAGGAAGTCATTGAAACCAACGCATTTGCAAGGATGCCAGAACTGAAACTACTCCATCTTAGTCACGTACAACTCGACGGATGTTATGCAGAATTTTGTACAGGAATAAGATGGATGTGTTGGACAAAATTTTCTTTGGATTCTATACCCTTTGATTTTCCTTTGGGGAGCCTAATTGTTCTTGAAATGCAATACAGTGGCTTGAGACAAATCTGTGAAGGAGCTAAACGTCTTCCGTTATTGAAGATCCTTGATCTCAGCCACTCTCATAGCCTTACTAATACTACTGACTTCTCATGTTGCCCCAATCTTGAGAAATTGGTTCTTGTAGATTGTGAAAGCCTGATTGGTGTCAATGAATCCATTGGAAGCCTAGAGAGACTTGTTTACTTGAGTTTGAGGGACTGCAAAAATCTTAAGATGCTTCCTAAGAATATCGTAATGCTAAAGTCACTTGAAACACTTATTGTATCTGGTTGCACAAATCTTAATCAGTTATCAATTGAGATGTTGAGGAACATGGCTCTGAAAGTCCTTGGGATAGATGGAATACCATTAGGTGAATTCTGGCCGGGAAGAAGTTTATCTATCTTGAGTTGTTTACCATGCTCTTTAGTAGACTTAAGTCTTGAGGGGTGCAGTCTTTTAGATGGTGTCTTTCCTAGGGATTTTAGTAGTCTATCCTCATTGCGAAGACTAAATCTAGCGAACAATCCAATTTGTAGTCTACCAAATTGCATCGGAGGTTTAGAGGGGCTCATTGATCTCTCTTTTTCCTGCTGTGCAAATCTCGAATCGCTGGTGGGGTTACCAAAAGTACATCATCTGGATCTGGGGTGTTGTGTATCATTAAAAAAAATAACATATAAATCCTCTGAGTTCAAATCTCACATTACCAGTGGTTTTGACAACCACAAACTAGTTGAGTGGGAATATAACTACAAGTTAGAACCCATTGGAAGAGTTGATGTAGACATGATCAACCTTTTGGGCTTGTGCAACTTGGAATCCATGGCGCCAATTTGGATTCGAAAACCGTACAATTCTGAAATTGTTGCTGAATTGAGTCCTGTCCAGGGACTGTACGAGCGTGGTATATTCACCACATTTTTTGCTGGGAATGAGGTTCCAGGCCAATTCATCCATAAAACTAAAGGGTCCTCATTATCTTTTACGGTTCCTTCACTTGATAATCACAGAATTGGAGGCTTGAAGGTCTTTAGTGTCTATACAAAACGTGCCAATGATTCTCCTTGGGCTTTACCCGGGCCAATGATTACTAGAGTCACAAATAAGAGTAAGGATATCAAGTTAATCTATGCCCCATCACACTGCGGCATTCCAGCTGAAGGAGAAGACATGATATGGTTAAGCCATTGGAAGTTGGAGGAGGAGGTTCACGTAGATGTCGGCGATAAACTGGTTGTTTCTGTAATTATGGAACCTTGGCTTCAGGTAAAGGAGTTTGGCATCCAGCTTGTGCAACCGCTGCAAAGTACCCAACACCAAACCATTGCTCCTTATCAGCAAGGAGTGCCAGGAGTATACTTGCTCTCACGCAAAAATGTACCCATACAAGATCCGATTTGGTTCAGTAAGATCCTTGGGGACTCTGATGAAGAAGATAGGACAGACAAAGAAGAAGAGAAAGTACAGGATGAGCAAACAATTGCAGCAGCCATGACAGCCGGCAATATTAACTCCAGAGGCCTCCTCCGTGGCTGGAAAGTGCTCATCACCGCTGCTTGCTTCTTTCTCGTACTTCTCATATCAGTCGCTCTTCTTTACTCTCTCACAGAAAGAAGGGACAGTCTAAAAGCTGTGGATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1173

Amino Acids

132.33

Weight (kDa)

6.43

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
AccB1I GGYRCC 2 cut(s) 273, 2701
AccI GTMKAC 5 cut(s) 49, 2304, 2413, 2658, 3231
AciI CCGC 8 cut(s) 425, 470, 586, 1133, 1179, 3021, 3173, 3426
AcoI YGGCCR 2 cut(s) 1120, 2255
AcsI RAATTY 5 cut(s) 740, 777, 1778, 1814, 2249
AcuI CTGAAG 3 cut(s) 1277, 3054, 3122
AcvI CACGTG 1 cut(s) 67
AcyI GRCGYC 2 cut(s) 1576, 2702
AfiI CCNNNNNNNGG 3 cut(s) 369, 2762, 3035
AflII CTTAAG 3 cut(s) 1102, 2102, 2308
AflIII ACRYGT 1 cut(s) 66
AhdI GACNNNNNGTC 2 cut(s) 259, 384
AhlI ACTAGT 1 cut(s) 2605
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 6 cut(s) 988, 1122, 1641, 2760, 2815, 3223
AjuI GAANNNNNNNTTGG 2 cut(s) 2016, 2048
Alw21I GWGCWC 3 cut(s) 1101, 1255, 3420
Alw26I GTCTC 4 cut(s) 153, 1676, 1885, 2059
Alw44I GTGCAC 1 cut(s) 1251
AlwNI CAGNNNCTG 1 cut(s) 2767
Ama87I CYCGRG 1 cut(s) 2951
AoxI GGCC 5 cut(s) 1120, 2255, 2818, 2954, 3394
ApaLI GTGCAC 1 cut(s) 1251
ApeKI GCWGC 6 cut(s) 17, 753, 3175, 3359, 3362, 3428
ApoI RAATTY 5 cut(s) 740, 777, 1778, 1814, 2249
Asp700I GAANNNNTTC 2 cut(s) 1272, 1530
AspA2I CCTAGG 1 cut(s) 2348
AspLEI GCGC 1 cut(s) 2704
AspS9I GGNCC 6 cut(s) 312, 946, 1126, 1171, 2844, 2954
AsuC2I CCSGG 3 cut(s) 2259, 2952, 2953
AsuHPI GGTGA 6 cut(s) 22, 304, 1295, 2258, 2779, 3415
AsuII TTCGAA 2 cut(s) 248, 2716
AvaI CYCGRG 1 cut(s) 2951
AvaII GGWCC 5 cut(s) 312, 946, 1126, 1171, 2844
AvrII CCTAGG 1 cut(s) 2348
BaeGI GKGCMC 1 cut(s) 1255
BalI TGGCCA 1 cut(s) 1122
BamHI GGATCC 1 cut(s) 355
BanI GGYRCC 2 cut(s) 273, 2701
BanII GRGCYC 2 cut(s) 1101, 2445
BarI GAAGNNNNNNTAC 4 cut(s) 840, 872, 3321, 3353
BbrPI CACGTG 1 cut(s) 67
BbsI GAAGAC 6 cut(s) 96, 704, 1206, 1910, 2386, 3048
Bbv12I GWGCWC 3 cut(s) 1101, 1255, 3420
BbvI GCAGC 6 cut(s) 29, 765, 3162, 3371, 3374, 3415
BciT130I CCWGG 6 cut(s) 990, 1124, 1643, 2762, 2817, 3225
BclI TGATCA 1 cut(s) 2664
BcnI CCSGG 3 cut(s) 2259, 2952, 2953
BcoDI GTCTC 4 cut(s) 153, 1676, 1885, 2059
BcuI ACTAGT 1 cut(s) 2605
BfoI RGCGCY 1 cut(s) 2705
BfrI CTTAAG 3 cut(s) 1102, 2102, 2308
BisI GCNGC 7 cut(s) 18, 754, 3022, 3176, 3360, 3363, 3429
BlnI CCTAGG 1 cut(s) 2348
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 7 cut(s) 19, 755, 3023, 3177, 3361, 3364, 3430
Bme1390I CCNGG 9 cut(s) 990, 1124, 1643, 2259, 2762, 2817, 2952, 2953, 3225
Bme18I GGWCC 5 cut(s) 312, 946, 1126, 1171, 2844
BmeRI GACNNNNNGTC 2 cut(s) 259, 384
BmeT110I CYCGRG 1 cut(s) 2951
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 6 cut(s) 312, 946, 1126, 1171, 2844, 2954
BmiI GGNNCC 8 cut(s) 275, 357, 1856, 2703, 2814, 2845, 2865, 3129
BmrFI CCNGG 9 cut(s) 990, 1124, 1643, 2259, 2762, 2817, 2952, 2953, 3225
BmsI GCATC 7 cut(s) 442, 1027, 1485, 1710, 2097, 2433, 3165
BoxI GACNNNNGTC 1 cut(s) 2310
BpiI GAAGAC 6 cut(s) 96, 704, 1206, 1910, 2386, 3048
BplI GAGNNNNNCTC 3 cut(s) 36, 3384, 3416
BpmI CTGGAG 3 cut(s) 224, 449, 3373
Bpu1102I GCTNAGC 1 cut(s) 21
Bpu14I TTCGAA 2 cut(s) 248, 2716
BpuEI CTTGAG 6 cut(s) 194, 1909, 2018, 2098, 2299, 2336
BpuMI CCSGG 3 cut(s) 2259, 2952, 2953
Bsa29I ATCGAT 2 cut(s) 979, 1473
BsaAI YACGTR 3 cut(s) 67, 1753, 3088
BsaBI GATNNNNATC 1 cut(s) 936
BsaHI GRCGYC 2 cut(s) 1576, 2702
BsaXI ACNNNNNCTCC 4 cut(s) 900, 930, 3187, 3217
Bsc4I CCNNNNNNNGG 3 cut(s) 369, 2762, 3035
Bse118I RCCGGY 1 cut(s) 3374
Bse1I ACTGG 4 cut(s) 12, 207, 2583, 3111
Bse3DI GCAATG 5 cut(s) 705, 1174, 1356, 2372, 3198
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 6 cut(s) 990, 1124, 1643, 2762, 2817, 3225
BseCI ATCGAT 2 cut(s) 979, 1473
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 3 cut(s) 369, 2762, 3035
BseMI GCAATG 5 cut(s) 705, 1174, 1356, 2372, 3198
BseMII CTCAG 8 cut(s) 35, 65, 330, 551, 873, 1044, 1957, 2553
BseNI ACTGG 4 cut(s) 12, 207, 2583, 3111
BseRI GAGGAG 5 cut(s) 22, 195, 3089, 3092, 3389
BseSI GKGCMC 1 cut(s) 1255
BseXI GCAGC 6 cut(s) 29, 765, 3162, 3371, 3374, 3415
BseYI CCCAGC 1 cut(s) 2801
BsgI GTGCAG 2 cut(s) 1511, 2344
BshFI GGCC 5 cut(s) 1122, 2257, 2820, 2956, 3396
BshNI GGYRCC 2 cut(s) 273, 2701
BshVI ATCGAT 2 cut(s) 979, 1473
BsiHKAI GWGCWC 3 cut(s) 1101, 1255, 3420
BsiHKCI CYCGRG 1 cut(s) 2951
BsiSI CCGG 3 cut(s) 2258, 2952, 3375
BslFI GGGAC 5 cut(s) 856, 2102, 2778, 3308, 3506
BslI CCNNNNNNNGG 3 cut(s) 369, 2762, 3035
BsmAI GTCTC 4 cut(s) 153, 1676, 1885, 2059
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 5 cut(s) 856, 2102, 2778, 3308, 3506
BsmI GAATGC 2 cut(s) 606, 3024
BsnI GGCC 5 cut(s) 1122, 2257, 2820, 2956, 3396
BsoBI CYCGRG 1 cut(s) 2951
Bsp119I TTCGAA 2 cut(s) 248, 2716
Bsp1286I GDGCHC 4 cut(s) 1101, 1255, 2445, 3420
Bsp1407I TGTACA 1 cut(s) 1784
Bsp1720I GCTNAGC 1 cut(s) 21
Bsp19I CCATGG 1 cut(s) 2697
BspACI CCGC 8 cut(s) 425, 470, 586, 1133, 1179, 3021, 3173, 3426
BspANI GGCC 5 cut(s) 1122, 2257, 2820, 2956, 3396
BspCNI CTCAG 8 cut(s) 34, 66, 329, 552, 874, 1045, 1956, 2554
BspDI ATCGAT 2 cut(s) 979, 1473
BspLI GGNNCC 8 cut(s) 275, 357, 1856, 2703, 2814, 2845, 2865, 3129
BspQI GCTCTTC 1 cut(s) 3471
BspT104I TTCGAA 2 cut(s) 248, 2716
BspT107I GGYRCC 2 cut(s) 273, 2701
BspTI CTTAAG 3 cut(s) 1102, 2102, 2308
BsrDI GCAATG 5 cut(s) 705, 1174, 1356, 2372, 3198
BsrFI RCCGGY 1 cut(s) 3374
BsrGI TGTACA 1 cut(s) 1784
BsrI ACTGG 4 cut(s) 12, 207, 2583, 3111
BssAI RCCGGY 1 cut(s) 3374
BssNAI GTATAC 1 cut(s) 3232
BssNI GRCGYC 2 cut(s) 1576, 2702
BssT1I CCWWGG 7 cut(s) 129, 2221, 2348, 2697, 2939, 3131, 3289
Bst1107I GTATAC 1 cut(s) 3232
Bst2UI CCWGG 6 cut(s) 990, 1124, 1643, 2762, 2817, 3225
Bst4CI ACNGT 6 cut(s) 863, 1883, 2724, 2769, 2863, 3497
Bst6I CTCTTC 6 cut(s) 40, 180, 1516, 2641, 3324, 3471
BstACI GRCGYC 2 cut(s) 1576, 2702
BstAFI CTTAAG 3 cut(s) 1102, 2102, 2308
BstAPI GCANNNNNTGC 3 cut(s) 467, 3175, 3220
BstAUI TGTACA 1 cut(s) 1784
BstBAI YACGTR 3 cut(s) 67, 1753, 3088
BstBI TTCGAA 2 cut(s) 248, 2716
BstC8I GCNNGC 5 cut(s) 1062, 1113, 1559, 3376, 3433
BstDSI CCRYGG 2 cut(s) 2697, 3403
BstEII GGTNACC 1 cut(s) 2493
BstH2I RGCGCY 1 cut(s) 2705
BstHHI GCGC 1 cut(s) 2704
BstMAI GTCTC 4 cut(s) 153, 1676, 1885, 2059
BstMWI GCNNNNNNNGC 6 cut(s) 467, 476, 1558, 3175, 3220, 3371
BstNI CCWGG 6 cut(s) 990, 1124, 1643, 2762, 2817, 3225
BstNSI RCATGY 4 cut(s) 719, 1064, 1115, 1651
BstPAI GACNNNNGTC 1 cut(s) 2310
BstPI GGTNACC 1 cut(s) 2493
BstSCI CCNGG 9 cut(s) 988, 1122, 1641, 2257, 2760, 2815, 2950, 2951, 3223
BstSLI GKGCMC 1 cut(s) 1255
BstV1I GCAGC 6 cut(s) 29, 765, 3162, 3371, 3374, 3415
BstV2I GAAGAC 6 cut(s) 96, 704, 1206, 1910, 2386, 3048
BstX2I RGATCY 6 cut(s) 355, 472, 1932, 2515, 3265, 3285
BstYI RGATCY 6 cut(s) 355, 472, 1932, 2515, 3265, 3285
BstZ17I GTATAC 1 cut(s) 3232
Bsu15I ATCGAT 2 cut(s) 979, 1473
BsuRI GGCC 5 cut(s) 1122, 2257, 2820, 2956, 3396
BsuTUI ATCGAT 2 cut(s) 979, 1473
BtgI CCRYGG 2 cut(s) 2697, 3403
BtrI CACGTC 1 cut(s) 352
BtsI GCAGTG 1 cut(s) 3016
BtsIMutI CAGTG 6 cut(s) 5, 108, 1253, 1888, 2590, 3016
Cac8I GCNNGC 5 cut(s) 1062, 1113, 1559, 3376, 3433
CaiI CAGNNNCTG 1 cut(s) 2767
CfoI GCGC 1 cut(s) 2704
Cfr10I RCCGGY 1 cut(s) 3374
Cfr13I GGNCC 6 cut(s) 312, 946, 1126, 1171, 2844, 2954
Cfr9I CCCGGG 1 cut(s) 2951
ClaI ATCGAT 2 cut(s) 979, 1473
CseI GACGC 2 cut(s) 249, 1565
CspCI CAANNNNNGTGG 4 cut(s) 1343, 1378, 2779, 2814
DinI GGCGCC 1 cut(s) 2703
DriI GACNNNNNGTC 2 cut(s) 259, 384
EaeI YGGCCR 2 cut(s) 1120, 2255
Eam1104I CTCTTC 6 cut(s) 40, 180, 1516, 2641, 3324, 3471
Eam1105I GACNNNNNGTC 2 cut(s) 259, 384
EarI CTCTTC 6 cut(s) 40, 180, 1516, 2641, 3324, 3471
Ecl136II GAGCTC 1 cut(s) 1099
Eco130I CCWWGG 7 cut(s) 129, 2221, 2348, 2697, 2939, 3131, 3289
Eco147I AGGCCT 1 cut(s) 3396
Eco24I GRGCYC 2 cut(s) 1101, 2445
Eco32I GATATC 1 cut(s) 2992
Eco47I GGWCC 5 cut(s) 312, 946, 1126, 1171, 2844
Eco53kI GAGCTC 1 cut(s) 1099
Eco57I CTGAAG 3 cut(s) 1277, 3054, 3122
Eco72I CACGTG 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 2951
Eco91I GGTNACC 1 cut(s) 2493
EcoICRI GAGCTC 1 cut(s) 1099
EcoO109I RGGNCCY 2 cut(s) 312, 2844
EcoO65I GGTNACC 1 cut(s) 2493
EcoRI GAATTC 1 cut(s) 2249
EcoRII CCWGG 6 cut(s) 988, 1122, 1641, 2760, 2815, 3223
EcoRV GATATC 1 cut(s) 2992
EcoT14I CCWWGG 7 cut(s) 129, 2221, 2348, 2697, 2939, 3131, 3289
EcoT22I ATGCAT 2 cut(s) 1500, 1653
EcoT38I GRGCYC 2 cut(s) 1101, 2445
EgeI GGCGCC 1 cut(s) 2703
EheI GGCGCC 1 cut(s) 2703
ErhI CCWWGG 7 cut(s) 129, 2221, 2348, 2697, 2939, 3131, 3289
Esp3I CGTCTC 1 cut(s) 153
FaqI GGGAC 5 cut(s) 856, 2102, 2778, 3308, 3506
FauNDI CATATG 1 cut(s) 1653
FbaI TGATCA 1 cut(s) 2664
FblI GTMKAC 5 cut(s) 49, 2304, 2413, 2658, 3231
Fnu4HI GCNGC 7 cut(s) 18, 754, 3022, 3176, 3360, 3363, 3429
FriOI GRGCYC 2 cut(s) 1101, 2445
Fsp4HI GCNGC 7 cut(s) 18, 754, 3022, 3176, 3360, 3363, 3429
GlaI GCGC 1 cut(s) 2703
GluI GCNGC 7 cut(s) 18, 754, 3022, 3176, 3360, 3363, 3429
GsaI CCCAGC 1 cut(s) 2805
GsuI CTGGAG 3 cut(s) 224, 449, 3373
HaeII RGCGCY 1 cut(s) 2705
HaeIII GGCC 5 cut(s) 1122, 2257, 2820, 2956, 3396
HapII CCGG 3 cut(s) 2258, 2952, 3375
HgaI GACGC 2 cut(s) 249, 1565
HhaI GCGC 1 cut(s) 2704
Hin1I GRCGYC 2 cut(s) 1576, 2702
Hin6I GCGC 1 cut(s) 2702
HinP1I GCGC 1 cut(s) 2702
HincII GTYRAC 3 cut(s) 50, 136, 583
HindII GTYRAC 3 cut(s) 50, 136, 583
HindIII AAGCTT 1 cut(s) 1550
HpaII CCGG 3 cut(s) 2258, 2952, 3375
HphI GGTGA 6 cut(s) 22, 304, 1295, 2258, 2779, 3415
Hpy99I CGWCG 6 cut(s) 51, 167, 265, 284, 353, 1766
HpyAV CCTTC 8 cut(s) 433, 740, 1625, 1898, 2877, 2893, 3029, 3483
HpyCH4III ACNGT 6 cut(s) 863, 1883, 2724, 2769, 2863, 3497
HpyCH4IV ACGT 6 cut(s) 66, 351, 1752, 1915, 2923, 3087
HpyF10VI GCNNNNNNNGC 6 cut(s) 467, 476, 1558, 3175, 3220, 3371
HpySE526I ACGT 6 cut(s) 66, 351, 1752, 1915, 2923, 3087
Hsp92I GRCGYC 2 cut(s) 1576, 2702
HspAI GCGC 1 cut(s) 2702
KasI GGCGCC 1 cut(s) 2701
KroI GCCGGC 1 cut(s) 3374
KroNI GCCGGC 1 cut(s) 3376
Ksp22I TGATCA 1 cut(s) 2664
LguI GCTCTTC 1 cut(s) 3471
LmnI GCTCC 5 cut(s) 1096, 1662, 1854, 1907, 3208
Lsp1109I GCAGC 6 cut(s) 29, 765, 3162, 3371, 3374, 3415
LweI GCATC 7 cut(s) 442, 1027, 1485, 1710, 2097, 2433, 3165
MaeII ACGT 6 cut(s) 66, 351, 1752, 1915, 2923, 3087
MaeIII GTNAC 9 cut(s) 633, 1006, 1396, 1432, 1465, 1748, 2136, 2493, 2971
MfeI CAATTG 2 cut(s) 2187, 3354
MflI RGATCY 6 cut(s) 355, 472, 1932, 2515, 3265, 3285
MhlI GDGCHC 4 cut(s) 1101, 1255, 2445, 3420
MlsI TGGCCA 1 cut(s) 1122
MluNI TGGCCA 1 cut(s) 1122
Mly113I GGCGCC 1 cut(s) 2702
MlyI GAGTC 6 cut(s) 571, 1310, 1678, 2761, 2979, 3290
MmeI TCCRAC 3 cut(s) 1245, 1787, 3051
Mox20I TGGCCA 1 cut(s) 1122
Mph1103I ATGCAT 2 cut(s) 1500, 1653
MroNI GCCGGC 1 cut(s) 3374
MroXI GAANNNNTTC 2 cut(s) 1272, 1530
MscI TGGCCA 1 cut(s) 1122
MslI CAYNNNNRTG 2 cut(s) 1652, 3090
Msp20I TGGCCA 1 cut(s) 1122
MspA1I CMGCKG 4 cut(s) 427, 3032, 3175, 3428
MspCI CTTAAG 3 cut(s) 1102, 2102, 2308
MspI CCGG 3 cut(s) 2258, 2952, 3375
MspR9I CCNGG 9 cut(s) 990, 1124, 1643, 2259, 2762, 2817, 2952, 2953, 3225
MunI CAATTG 2 cut(s) 2187, 3354
Mva1269I GAATGC 2 cut(s) 606, 3024
MvaI CCWGG 6 cut(s) 990, 1124, 1643, 2762, 2817, 3225
MwoI GCNNNNNNNGC 6 cut(s) 467, 476, 1558, 3175, 3220, 3371
NaeI GCCGGC 1 cut(s) 3376
NarI GGCGCC 1 cut(s) 2702
NciI CCSGG 3 cut(s) 2259, 2952, 2953
NcoI CCATGG 1 cut(s) 2697
NdeI CATATG 1 cut(s) 1653
NgoMIV GCCGGC 1 cut(s) 3374
NlaIV GGNNCC 8 cut(s) 275, 357, 1856, 2703, 2814, 2845, 2865, 3129
NmuCI GTSAC 4 cut(s) 1432, 1748, 2136, 2971
NsiI ATGCAT 2 cut(s) 1500, 1653
NspI RCATGY 4 cut(s) 719, 1064, 1115, 1651
NspV TTCGAA 2 cut(s) 248, 2716
PaeI GCATGC 2 cut(s) 1064, 1115
PceI AGGCCT 1 cut(s) 3396
PciSI GCTCTTC 1 cut(s) 3471
PcsI WCGNNNNNNNCGW 1 cut(s) 1758
PctI GAATGC 2 cut(s) 606, 3024
PdiI GCCGGC 1 cut(s) 3376
PdmI GAANNNNTTC 2 cut(s) 1272, 1530
PflFI GACNNNGTC 2 cut(s) 54, 326
PfoI TCCNGGA 2 cut(s) 988, 1641
PkrI GCNGC 7 cut(s) 19, 755, 3023, 3177, 3361, 3364, 3430
PleI GAGTC 6 cut(s) 570, 1310, 1677, 2760, 2978, 3290
PluTI GGCGCC 1 cut(s) 2705
PmaCI CACGTG 1 cut(s) 67
PmlI CACGTG 1 cut(s) 67
PpsI GAGTC 6 cut(s) 570, 1310, 1677, 2760, 2978, 3290
Ppu21I YACGTR 3 cut(s) 67, 1753, 3088
PpuMI RGGWCCY 2 cut(s) 312, 2844
PshAI GACNNNNGTC 1 cut(s) 2310
PsiI TTATAA 1 cut(s) 723
Psp124BI GAGCTC 1 cut(s) 1101
Psp5II RGGWCCY 2 cut(s) 312, 2844
Psp6I CCWGG 6 cut(s) 988, 1122, 1641, 2760, 2815, 3223
PspCI CACGTG 1 cut(s) 67
PspEI GGTNACC 1 cut(s) 2493
PspFI CCCAGC 1 cut(s) 2801
PspGI CCWGG 6 cut(s) 988, 1122, 1641, 2760, 2815, 3223
PspN4I GGNNCC 8 cut(s) 275, 357, 1856, 2703, 2814, 2845, 2865, 3129
PspPI GGNCC 6 cut(s) 312, 946, 1126, 1171, 2844, 2954
PspPPI RGGWCCY 2 cut(s) 312, 2844
PsrI GAACNNNNNNTAC 2 cut(s) 1719, 1751
PstNI CAGNNNCTG 1 cut(s) 2767
PsuI RGATCY 6 cut(s) 355, 472, 1932, 2515, 3265, 3285
PsyI GACNNNGTC 2 cut(s) 54, 326
PvuII CAGCTG 1 cut(s) 3032
RseI CAYNNNNRTG 2 cut(s) 1652, 3090
SacI GAGCTC 1 cut(s) 1101
SalI GTCGAC 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 3471
SatI GCNGC 7 cut(s) 18, 754, 3022, 3176, 3360, 3363, 3429
Sau96I GGNCC 6 cut(s) 312, 946, 1126, 1171, 2844, 2954
SchI GAGTC 6 cut(s) 571, 1310, 1678, 2761, 2979, 3290
ScrFI CCNGG 9 cut(s) 990, 1124, 1643, 2259, 2762, 2817, 2952, 2953, 3225
SduI GDGCHC 4 cut(s) 1101, 1255, 2445, 3420
SfaNI GCATC 7 cut(s) 442, 1027, 1485, 1710, 2097, 2433, 3165
SfoI GGCGCC 1 cut(s) 2703
SfuI TTCGAA 2 cut(s) 248, 2716
SinI GGWCC 5 cut(s) 312, 946, 1126, 1171, 2844
SmaI CCCGGG 1 cut(s) 2953
SmiMI CAYNNNNRTG 2 cut(s) 1652, 3090
SmlI CTYRAG 9 cut(s) 173, 1102, 1888, 1997, 2077, 2102, 2278, 2308, 2315
SmoI CTYRAG 9 cut(s) 173, 1102, 1888, 1997, 2077, 2102, 2278, 2308, 2315
SpeI ACTAGT 1 cut(s) 2605
SphI GCATGC 2 cut(s) 1064, 1115
SseBI AGGCCT 1 cut(s) 3396
SsiI CCGC 8 cut(s) 425, 470, 586, 1133, 1179, 3021, 3173, 3426
SspDI GGCGCC 1 cut(s) 2701
SspI AATATT 1 cut(s) 3382
SstI GAGCTC 1 cut(s) 1101
StuI AGGCCT 1 cut(s) 3396
StyD4I CCNGG 9 cut(s) 988, 1122, 1641, 2257, 2760, 2815, 2950, 2951, 3223
StyI CCWWGG 7 cut(s) 129, 2221, 2348, 2697, 2939, 3131, 3289
TaaI ACNGT 6 cut(s) 863, 1883, 2724, 2769, 2863, 3497
TaiI ACGT 6 cut(s) 69, 354, 1755, 1918, 2926, 3090
TatI WGTACW 4 cut(s) 961, 1784, 2503, 3337
TauI GCSGC 1 cut(s) 3024
TscAI CASTG 6 cut(s) 12, 115, 1260, 1888, 2590, 3023
TseFI GTSAC 4 cut(s) 1432, 1748, 2136, 2971
TseI GCWGC 6 cut(s) 17, 753, 3175, 3359, 3362, 3428
Tsp45I GTSAC 4 cut(s) 1432, 1748, 2136, 2971
TspDTI ATGAA 5 cut(s) 986, 1491, 2058, 2815, 3318
TspGWI ACGGA 4 cut(s) 1179, 1779, 1911, 3392
TspMI CCCGGG 1 cut(s) 2951
TspRI CASTG 6 cut(s) 12, 115, 1260, 1888, 2590, 3023
Tth111I GACNNNGTC 2 cut(s) 54, 326
Vha464I CTTAAG 3 cut(s) 1102, 2102, 2308
VneI GTGCAC 1 cut(s) 1251
VpaK11BI GGWCC 5 cut(s) 312, 946, 1126, 1171, 2844
XapI RAATTY 5 cut(s) 740, 777, 1778, 1814, 2249
XbaI TCTAGA 1 cut(s) 1537
XceI RCATGY 4 cut(s) 719, 1064, 1115, 1651
XmaI CCCGGG 1 cut(s) 2951
XmaJI CCTAGG 1 cut(s) 2348
XmiI GTMKAC 5 cut(s) 49, 2304, 2413, 2658, 3231
XmnI GAANNNNTTC 2 cut(s) 1272, 1530
Zsp2I ATGCAT 2 cut(s) 1500, 1653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.