Rmu_sc0001992.1_g000022

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001992.1
OG0000036
Physical Location & Seq
Forward (+)
96195 .. 102053
5859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001992.1_g000022.1.cds

Sequence Viewer

Length: 3183 bp
atggctgatagtcgtctattggggggcaatagacctctattgctccgggcaatagatgtctattgggggcaaaaaaacatttccggtgagatttttagcaaattccggtgggcggcggcgggtgaccaggttcaggcggccgatgaccggaatccggcgaccggtgaccaggctccggcgaagtctcctatgtttgtcagttctaggatggctcttgcacttgcaacaactatctctccgaatacttgtccaactagttatcatgtgttcttgagtttcaggggtgatactcgcaagacttttactgaccacctctacacagcctttgtcaattcaggctttcgtacgtttcgagatgacgacgaacttccaaggggacatgatatcaaaccagaactgcggaaagccatcgagcagtcacgaagttctgttattgtgttttcacaagactatgcgtcttccggatggtgccttgatgagcttgtgatgatcatggaacgcaagaggacccgcgatcacatagttttacctgtgttttacgatgtcgatccatcccaagtaaggaagcaaacaggaagtcttgcaaaagcatttgctagacacgaaagaaatcattccttgaaggacaaggtgaagcgatggagggcggcactcacagaagttgcagatctagcaggaatggtcttgcaaaatgaagccgacgggcacgagtctaggtttatcaagaaaattgtaagggtaatgaaagataaactaaggcgcattcccttaagtgttgaaccccacctaattggagtagattctcaaacagaaggcatcaatttatggttacaggatggatcagctgatgttggcatacttttaattcatgggatgcgtgggataggaaaatcaaccatagcgaaatttgtttacaactcaaactttcaaaaatttgagagatgcagtttccttgaaaatgtcagagaagtttcagaacaatcaaatggcttgcttcggctacaaaaacaacttcttagtgatattttgggtgcaaatgaagtgaaaatacacaatattagtgaagggatggctaagattgaagattctattagctctgaaagagtttttcttgttctcgatgatgttgatcatgtggaccaattagctgcattcctgaggatgcaaaatcggtttcatccaggaagtaagatcatcataacaagtacctgtgcgggattgttgggagctcactgccaatttgctaagttgaatgaggttagaattctgggtgaggatgattcattagcgctctttagttggcatgcttttggacaggaccatcccattggaagttatactgattactcgaaaagggtaatagatcattgtggaggacttccactagctcttaaagttttaggttcttctttatcaggaaagaatctagttgtctgggaaagtgcattgaacaaactggaagctattccaaatggtgaaataatgaagaaactcaaaataagctatgattccctacaagatgatcatgacagaaacttattccttcatattgcattctttttcatcggaatggagaaaggtatcattgttacaatcttagatgactgtgatttcttcacagaggttggcattcaaaatctcattgatagatgtttggtaaccactgataaagataacaagcttgtgcaaatgcatgacatgattcgtgacatgggaagagaaattgttcgtctagaatccaaggagccagaacgacgtagtcgaggcacccaaacaattgaaggacttgttctcaacatgcaaatgtaccattctagaaattcaagtaaggtagtattggaaaccaatgcatttacaaggatgcacaaactaagactcctccaactcagtcatgtgcagctccgtggacgctacgaagaatttcctgcaggactaagatggctttgttggattaaatttcctttagcttccttgcccgctgattttccgttggagagccttgttgctcttgaaatgtgttatagtagtttgagacgactttggaggggaacaaagtatctcccatcacttaaaatcttaaatctcagccactctcatgactttactgaaatatttgacttctcatgtatccctaatctagagagactcattcttgaagattgtgcaagattgataaatgttcatgaatccatcggagtcctgaagaggcttatttatttgagtatgaaagattgcaaaaatcttaggaagcttccggagaacatttctatcctaaccttccttgaaacacttaatatctctggttgctcaaatcttagagagtttccgacagaaatgatgaagatacagtcgttgaaagtgtttcaagcagatggagttccaatacatcggttactagtcactaccaccagggaggtcgtactgtgtccgactgtaaaactttcttgggcttcttatctaccacggaatttagtaaacttgagtctaaaaaactgcaatcttttggatgatgattttcctagtgatgttgcaaatctagtgtcgttgcaaaaattggatctcagcaggaacccaatttgtaggatgccggatttcatcacaggtgttaccggcctccaatatcttaatttggagcattgtacgaagctcaaatcactttcatggcaatcaacaaaattagttgaattgaagctgttcggctgcgcctcattggaaaatataagatatcaacctttaccgatgagacttgctcatggttcgaactctaatcttgttaagtttcaggatgtttttatgttggaacccattgaaagagttgataaagaaatgctaaaagttttgagcttaggaaacctggaatccctggaaaactttgtgatggacacagtatctgatgaaacctctatatggaaaggaagaatgcagcctctgcagggactgtacgaagatggtgcattcaccacattccttcctggaaacgaaattccaggccagttcagctatagaagcagagggtcctctgtatcctttactgtgcctttacttcccaatctcaatatacgagggctgaatatcttctcggtcatagcacaatccaacaccaatgattctgatcctatgaccaataatgtcaatattgatggttcatattatacgacattgaccgttgtgattagtgattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1060

Amino Acids

120.04

Weight (kDa)

7.34

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 468, 1766
AccII CGCG 1 cut(s) 513
AccIII TCCGGA 2 cut(s) 461, 2254
AciI CCGC 9 cut(s) 113, 116, 119, 137, 400, 511, 647, 1214, 1977
AclWI GGATC 4 cut(s) 542, 847, 2565, 3107
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 9 cut(s) 101, 905, 932, 1263, 1819, 1919, 1955, 2467, 2982
AcuI CTGAAG 1 cut(s) 2222
AfaI GTAC 6 cut(s) 346, 1207, 1808, 2421, 2641, 2942
AfeI AGCGCT 1 cut(s) 1290
AfiI CCNNNNNNNGG 9 cut(s) 112, 133, 147, 154, 161, 175, 561, 2907, 2933
AflII CTTAAG 1 cut(s) 769
AgeI ACCGGT 1 cut(s) 161
AhdI GACNNNNNGTC 1 cut(s) 454
AhlI ACTAGT 2 cut(s) 254, 2395
AjnI CCWGG 8 cut(s) 126, 168, 1180, 2408, 2853, 2862, 2971, 2986
Alw21I GWGCWC 1 cut(s) 1231
Alw26I GTCTC 4 cut(s) 189, 2026, 2137, 2737
AlwI GGATC 4 cut(s) 542, 847, 2565, 3107
AlwNI CAGNNNCTG 3 cut(s) 2891, 2929, 2938
Aor13HI TCCGGA 2 cut(s) 461, 2254
Aor51HI AGCGCT 1 cut(s) 1290
AoxI GGCC 3 cut(s) 138, 2611, 2989
ApeKI GCWGC 4 cut(s) 1148, 1897, 2700, 2923
ApoI RAATTY 9 cut(s) 101, 905, 932, 1263, 1819, 1919, 1955, 2467, 2982
ArsI GACNNNNNNTTYG 4 cut(s) 562, 594, 2525, 2557
AsiGI ACCGGT 1 cut(s) 161
Asp700I GAANNNNTTC 6 cut(s) 613, 1464, 1725, 1920, 2693, 3074
AspLEI GCGC 3 cut(s) 762, 1291, 2705
AspS9I GGNCC 4 cut(s) 507, 1138, 1318, 3015
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 8 cut(s) 98, 134, 176, 296, 643, 1283, 1487, 2950
AsuII TTCGAA 1 cut(s) 2759
AvaII GGWCC 4 cut(s) 507, 1138, 1318, 3015
AxyI CCTNAGG 1 cut(s) 1157
BaeGI GKGCMC 1 cut(s) 708
BaeI ACNNNNGTAYC 2 cut(s) 2336, 2369
BanI GGYRCC 2 cut(s) 468, 1766
BanII GRGCYC 1 cut(s) 1231
BarI GAAGNNNNNNTAC 2 cut(s) 1032, 1064
BauI CACGAG 1 cut(s) 707
BbsI GAAGAC 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 1231
BbvI GCAGC 4 cut(s) 1135, 1909, 2687, 2935
BcgI CGANNNNNNTGC 2 cut(s) 2933, 2967
BciT130I CCWGG 8 cut(s) 128, 170, 1182, 2410, 2855, 2864, 2973, 2988
BciVI GTATCC 2 cut(s) 2138, 3034
BclI TGATCA 3 cut(s) 489, 1129, 1522
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 4 cut(s) 189, 2026, 2137, 2737
BcuI ACTAGT 2 cut(s) 254, 2395
BfmI CTRYAG 3 cut(s) 1926, 2930, 3001
BfoI RGCGCY 1 cut(s) 1292
BfrI CTTAAG 1 cut(s) 769
BfuI GTATCC 2 cut(s) 2138, 3034
BglII AGATCT 1 cut(s) 667
BisI GCNGC 8 cut(s) 114, 117, 138, 648, 1149, 1898, 2701, 2924
BlsI GCNGC 8 cut(s) 115, 118, 139, 649, 1150, 1899, 2702, 2925
Bme1390I CCNGG 9 cut(s) 47, 128, 170, 1182, 2410, 2855, 2864, 2973, 2988
Bme18I GGWCC 4 cut(s) 507, 1138, 1318, 3015
BmeRI GACNNNNNGTC 1 cut(s) 454
BmgT120I GGNCC 4 cut(s) 507, 1138, 1318, 3015
BmiI GGNNCC 8 cut(s) 174, 470, 509, 1746, 1768, 2570, 2802, 3016
BmrFI CCNGG 9 cut(s) 47, 128, 170, 1182, 2410, 2855, 2864, 2973, 2988
BmsI GCATC 6 cut(s) 825, 864, 932, 1152, 1851, 2574
BpiI GAAGAC 1 cut(s) 450
BplI GAGNNNNNCTC 2 cut(s) 2734, 2766
Bpu10I CCTNAGC 1 cut(s) 2845
Bpu14I TTCGAA 1 cut(s) 2759
BpuEI CTTGAG 2 cut(s) 292, 2500
BpuMI CCSGG 1 cut(s) 47
BsaBI GATNNNNATC 3 cut(s) 546, 1128, 3111
BsaJI CCNNGG 6 cut(s) 371, 1740, 1903, 2409, 2462, 2862
BsaWI WCCGGW 6 cut(s) 83, 105, 147, 161, 461, 2254
BsaXI ACNNNNNCTCC 4 cut(s) 220, 250, 1860, 1890
Bsc4I CCNNNNNNNGG 9 cut(s) 112, 133, 147, 154, 161, 175, 561, 2907, 2933
Bse118I RCCGGY 2 cut(s) 161, 2609
Bse1I ACTGG 2 cut(s) 1461, 2992
Bse21I CCTNAGG 1 cut(s) 1157
Bse8I GATNNNNATC 3 cut(s) 546, 1128, 3111
BseAI TCCGGA 2 cut(s) 461, 2254
BseBI CCWGG 8 cut(s) 128, 170, 1182, 2410, 2855, 2864, 2973, 2988
BseDI CCNNGG 6 cut(s) 371, 1740, 1903, 2409, 2462, 2862
BseJI GATNNNNATC 3 cut(s) 546, 1128, 3111
BseLI CCNNNNNNNGG 9 cut(s) 112, 133, 147, 154, 161, 175, 561, 2907, 2933
BseMII CTCAG 4 cut(s) 1148, 1900, 2098, 2575
BseNI ACTGG 2 cut(s) 1461, 2992
BseRI GAGGAG 1 cut(s) 1868
BseSI GKGCMC 1 cut(s) 708
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 4 cut(s) 1135, 1909, 2687, 2935
BsgI GTGCAG 1 cut(s) 1916
Bsh1236I CGCG 1 cut(s) 513
Bsh1285I CGRYCG 2 cut(s) 141, 162
BshFI GGCC 3 cut(s) 140, 2613, 2991
BshNI GGYRCC 2 cut(s) 468, 1766
BshTI ACCGGT 1 cut(s) 161
BsiEI CGRYCG 2 cut(s) 141, 162
BsiHKAI GWGCWC 1 cut(s) 1231
BsiWI CGTACG 1 cut(s) 344
BslFI GGGAC 2 cut(s) 390, 2949
BslI CCNNNNNNNGG 9 cut(s) 112, 133, 147, 154, 161, 175, 561, 2907, 2933
BsmAI GTCTC 4 cut(s) 189, 2026, 2137, 2737
BsmBI CGTCTC 1 cut(s) 2026
BsmFI GGGAC 2 cut(s) 390, 2949
BsmI GAATGC 6 cut(s) 762, 1151, 1553, 1629, 2925, 2954
BsnI GGCC 3 cut(s) 140, 2613, 2991
Bsp119I TTCGAA 1 cut(s) 2759
Bsp1286I GDGCHC 2 cut(s) 708, 1231
Bsp13I TCCGGA 2 cut(s) 461, 2254
BspACI CCGC 9 cut(s) 113, 116, 119, 137, 400, 511, 647, 1214, 1977
BspANI GGCC 3 cut(s) 140, 2613, 2991
BspCNI CTCAG 4 cut(s) 1149, 1899, 2097, 2574
BspEI TCCGGA 2 cut(s) 461, 2254
BspFNI CGCG 1 cut(s) 513
BspHI TCATGA 3 cut(s) 1525, 2095, 2182
BspLI GGNNCC 8 cut(s) 174, 470, 509, 1746, 1768, 2570, 2802, 3016
BspMAI CTGCAG 2 cut(s) 1930, 2934
BspPI GGATC 4 cut(s) 542, 847, 2565, 3107
BspT104I TTCGAA 1 cut(s) 2759
BspT107I GGYRCC 2 cut(s) 468, 1766
BspTI CTTAAG 1 cut(s) 769
BsrFI RCCGGY 2 cut(s) 161, 2609
BsrI ACTGG 2 cut(s) 1461, 2992
BssAI RCCGGY 2 cut(s) 161, 2609
BssECI CCNNGG 6 cut(s) 371, 1740, 1903, 2409, 2462, 2862
BssSI CACGAG 1 cut(s) 707
BssT1I CCWWGG 2 cut(s) 371, 1740
Bst2BI CACGAG 1 cut(s) 707
Bst2UI CCWGG 8 cut(s) 128, 170, 1182, 2410, 2855, 2864, 2973, 2988
Bst4CI ACNGT 8 cut(s) 1607, 2349, 2424, 2434, 2887, 2940, 3034, 3166
Bst6I CTCTTC 2 cut(s) 1711, 2198
BstAFI CTTAAG 1 cut(s) 769
BstAPI GCANNNNNTGC 1 cut(s) 2929
BstBI TTCGAA 1 cut(s) 2759
BstC8I GCNNGC 3 cut(s) 992, 1305, 1977
BstDSI CCRYGG 2 cut(s) 1903, 2462
BstEII GGTNACC 3 cut(s) 122, 164, 1657
BstFNI CGCG 1 cut(s) 513
BstH2I RGCGCY 1 cut(s) 1292
BstHHI GCGC 3 cut(s) 762, 1291, 2705
BstMAI GTCTC 4 cut(s) 189, 2026, 2137, 2737
BstMCI CGRYCG 2 cut(s) 141, 162
BstMWI GCNNNNNNNGC 4 cut(s) 671, 2929, 2997, 3006
BstNI CCWGG 8 cut(s) 128, 170, 1182, 2410, 2855, 2864, 2973, 2988
BstNSI RCATGY 2 cut(s) 1307, 1801
BstPI GGTNACC 3 cut(s) 122, 164, 1657
BstSCI CCNGG 9 cut(s) 45, 126, 168, 1180, 2408, 2853, 2862, 2971, 2986
BstSFI CTRYAG 3 cut(s) 1926, 2930, 3001
BstSLI GKGCMC 1 cut(s) 708
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 4 cut(s) 1135, 1909, 2687, 2935
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 2 cut(s) 667, 2557
BstXI CCANNNNNNTGG 2 cut(s) 791, 1328
BstYI RGATCY 2 cut(s) 667, 2557
BstZI CGGCCG 1 cut(s) 138
Bsu36I CCTNAGG 1 cut(s) 1157
BsuI GTATCC 2 cut(s) 2138, 3034
BsuRI GGCC 3 cut(s) 140, 2613, 2991
BtgI CCRYGG 2 cut(s) 1903, 2462
BtgZI GCGATG 1 cut(s) 652
BtsI GCAGTG 1 cut(s) 1231
BtsIMutI CAGTG 2 cut(s) 1231, 1662
Cac8I GCNNGC 3 cut(s) 992, 1305, 1977
CaiI CAGNNNCTG 3 cut(s) 2891, 2929, 2938
CciI TCATGA 3 cut(s) 1525, 2095, 2182
CfoI GCGC 3 cut(s) 762, 1291, 2705
Cfr10I RCCGGY 2 cut(s) 161, 2609
Cfr13I GGNCC 4 cut(s) 507, 1138, 1318, 3015
CseI GACGC 2 cut(s) 444, 1917
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 6 cut(s) 345, 1206, 1807, 2420, 2640, 2941
CspAI ACCGGT 1 cut(s) 161
CviQI GTAC 6 cut(s) 345, 1206, 1807, 2420, 2640, 2941
DriI GACNNNNNGTC 1 cut(s) 454
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
Eam1104I CTCTTC 2 cut(s) 1711, 2198
Eam1105I GACNNNNNGTC 1 cut(s) 454
EarI CTCTTC 2 cut(s) 1711, 2198
Ecl136II GAGCTC 1 cut(s) 1229
EclXI CGGCCG 1 cut(s) 138
Eco130I CCWWGG 2 cut(s) 371, 1740
Eco24I GRGCYC 1 cut(s) 1231
Eco32I GATATC 2 cut(s) 385, 2726
Eco47I GGWCC 4 cut(s) 507, 1138, 1318, 3015
Eco47III AGCGCT 1 cut(s) 1290
Eco52I CGGCCG 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 1229
Eco57I CTGAAG 1 cut(s) 2222
Eco81I CCTNAGG 1 cut(s) 1157
Eco91I GGTNACC 3 cut(s) 122, 164, 1657
EcoICRI GAGCTC 1 cut(s) 1229
EcoO109I RGGNCCY 2 cut(s) 507, 3015
EcoO65I GGTNACC 3 cut(s) 122, 164, 1657
EcoRI GAATTC 1 cut(s) 1263
EcoRII CCWGG 8 cut(s) 126, 168, 1180, 2408, 2853, 2862, 2971, 2986
EcoRV GATATC 2 cut(s) 385, 2726
EcoT14I CCWWGG 2 cut(s) 371, 1740
EcoT22I ATGCAT 2 cut(s) 1695, 1852
EcoT38I GRGCYC 1 cut(s) 1231
ErhI CCWWGG 2 cut(s) 371, 1740
Esp3I CGTCTC 1 cut(s) 2026
FaqI GGGAC 2 cut(s) 390, 2949
FauI CCCGC 4 cut(s) 112, 518, 1207, 1984
FbaI TGATCA 3 cut(s) 489, 1129, 1522
Fnu4HI GCNGC 8 cut(s) 114, 117, 138, 648, 1149, 1898, 2701, 2924
FriOI GRGCYC 1 cut(s) 1231
Fsp4HI GCNGC 8 cut(s) 114, 117, 138, 648, 1149, 1898, 2701, 2924
GlaI GCGC 3 cut(s) 761, 1290, 2704
GluI GCNGC 8 cut(s) 114, 117, 138, 648, 1149, 1898, 2701, 2924
HaeII RGCGCY 1 cut(s) 1292
HaeIII GGCC 3 cut(s) 140, 2613, 2991
HgaI GACGC 2 cut(s) 444, 1917
HhaI GCGC 3 cut(s) 762, 1291, 2705
Hin6I GCGC 3 cut(s) 760, 1289, 2703
HinP1I GCGC 3 cut(s) 760, 1289, 2703
HindIII AAGCTT 2 cut(s) 1679, 2249
HphI GGTGA 8 cut(s) 98, 134, 176, 296, 643, 1283, 1487, 2950
Hpy166II GTNNAC 4 cut(s) 913, 1138, 1907, 2476
Hpy8I GTNNAC 4 cut(s) 913, 1138, 1907, 2476
Hpy99I CGWCG 3 cut(s) 365, 704, 1758
HpyAV CCTTC 7 cut(s) 616, 806, 1058, 1553, 1775, 2287, 2978
HpyCH4III ACNGT 8 cut(s) 1607, 2349, 2424, 2434, 2887, 2940, 3034, 3166
HpyCH4IV ACGT 2 cut(s) 347, 1756
HpyF10VI GCNNNNNNNGC 4 cut(s) 671, 2929, 2997, 3006
HpySE526I ACGT 2 cut(s) 347, 1756
HspAI GCGC 3 cut(s) 760, 1289, 2703
Kpn2I TCCGGA 2 cut(s) 461, 2254
Ksp22I TGATCA 3 cut(s) 489, 1129, 1522
LmnI GCTCC 6 cut(s) 48, 178, 1226, 1744, 1905, 2632
Lsp1109I GCAGC 4 cut(s) 1135, 1909, 2687, 2935
LweI GCATC 6 cut(s) 825, 864, 932, 1152, 1851, 2574
MabI ACCWGGT 1 cut(s) 126
MaeII ACGT 2 cut(s) 347, 1756
MfeI CAATTG 1 cut(s) 1776
MflI RGATCY 2 cut(s) 667, 2557
MhlI GDGCHC 2 cut(s) 708, 1231
MlyI GAGTC 5 cut(s) 719, 1869, 2139, 2205, 2491
MmeI TCCRAC 8 cut(s) 275, 1906, 1928, 1971, 2351, 2453, 2778, 3120
Mph1103I ATGCAT 2 cut(s) 1695, 1852
MroI TCCGGA 2 cut(s) 461, 2254
MroXI GAANNNNTTC 6 cut(s) 613, 1464, 1725, 1920, 2693, 3074
MslI CAYNNNNRTG 4 cut(s) 1568, 1802, 2094, 2659
MspA1I CMGCKG 2 cut(s) 845, 1979
MspCI CTTAAG 1 cut(s) 769
MspR9I CCNGG 9 cut(s) 47, 128, 170, 1182, 2410, 2855, 2864, 2973, 2988
MunI CAATTG 1 cut(s) 1776
Mva1269I GAATGC 6 cut(s) 762, 1151, 1553, 1629, 2925, 2954
MvaI CCWGG 8 cut(s) 128, 170, 1182, 2410, 2855, 2864, 2973, 2988
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 671, 2929, 2997, 3006
NciI CCSGG 1 cut(s) 47
NlaIV GGNNCC 8 cut(s) 174, 470, 509, 1746, 1768, 2570, 2802, 3016
NmuCI GTSAC 5 cut(s) 122, 164, 417, 1706, 2398
NsiI ATGCAT 2 cut(s) 1695, 1852
NspI RCATGY 2 cut(s) 1307, 1801
NspV TTCGAA 1 cut(s) 2759
PaeI GCATGC 1 cut(s) 1307
PagI TCATGA 3 cut(s) 1525, 2095, 2182
PcsI WCGNNNNNNNCGW 2 cut(s) 349, 1759
PctI GAATGC 6 cut(s) 762, 1151, 1553, 1629, 2925, 2954
PdmI GAANNNNTTC 6 cut(s) 613, 1464, 1725, 1920, 2693, 3074
Pfl23II CGTACG 1 cut(s) 344
PflFI GACNNNGTC 1 cut(s) 1758
PfoI TCCNGGA 2 cut(s) 1180, 2971
PinAI ACCGGT 1 cut(s) 161
PkrI GCNGC 8 cut(s) 115, 118, 139, 649, 1150, 1899, 2702, 2925
PleI GAGTC 5 cut(s) 718, 1869, 2139, 2204, 2490
PpsI GAGTC 5 cut(s) 718, 1869, 2139, 2204, 2490
PpuMI RGGWCCY 2 cut(s) 507, 3015
Psp124BI GAGCTC 1 cut(s) 1231
Psp5II RGGWCCY 2 cut(s) 507, 3015
Psp6I CCWGG 8 cut(s) 126, 168, 1180, 2408, 2853, 2862, 2971, 2986
PspEI GGTNACC 3 cut(s) 122, 164, 1657
PspGI CCWGG 8 cut(s) 126, 168, 1180, 2408, 2853, 2862, 2971, 2986
PspLI CGTACG 1 cut(s) 344
PspN4I GGNNCC 8 cut(s) 174, 470, 509, 1746, 1768, 2570, 2802, 3016
PspPI GGNCC 4 cut(s) 507, 1138, 1318, 3015
PspPPI RGGWCCY 2 cut(s) 507, 3015
PstI CTGCAG 2 cut(s) 1930, 2934
PstNI CAGNNNCTG 3 cut(s) 2891, 2929, 2938
PsuI RGATCY 2 cut(s) 667, 2557
PsyI GACNNNGTC 1 cut(s) 1758
PvuII CAGCTG 1 cut(s) 845
RsaI GTAC 6 cut(s) 346, 1207, 1808, 2421, 2641, 2942
RsaNI GTAC 6 cut(s) 345, 1206, 1807, 2420, 2640, 2941
RseI CAYNNNNRTG 4 cut(s) 1568, 1802, 2094, 2659
SacI GAGCTC 1 cut(s) 1231
SatI GCNGC 8 cut(s) 114, 117, 138, 648, 1149, 1898, 2701, 2924
Sau96I GGNCC 4 cut(s) 507, 1138, 1318, 3015
SbfI CCTGCAGG 1 cut(s) 1930
SchI GAGTC 5 cut(s) 719, 1869, 2139, 2205, 2491
ScrFI CCNGG 9 cut(s) 47, 128, 170, 1182, 2410, 2855, 2864, 2973, 2988
SdaI CCTGCAGG 1 cut(s) 1930
SduI GDGCHC 2 cut(s) 708, 1231
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 6 cut(s) 825, 864, 932, 1152, 1851, 2574
SfcI CTRYAG 3 cut(s) 1926, 2930, 3001
SfuI TTCGAA 1 cut(s) 2759
SinI GGWCC 4 cut(s) 507, 1138, 1318, 3015
SmiMI CAYNNNNRTG 4 cut(s) 1568, 1802, 2094, 2659
SmlI CTYRAG 3 cut(s) 271, 769, 2479
SmoI CTYRAG 3 cut(s) 271, 769, 2479
SpeI ACTAGT 2 cut(s) 254, 2395
SphI GCATGC 1 cut(s) 1307
Sse8387I CCTGCAGG 1 cut(s) 1930
SsiI CCGC 9 cut(s) 113, 116, 119, 137, 400, 511, 647, 1214, 1977
SspI AATATT 3 cut(s) 1057, 2112, 3136
SstI GAGCTC 1 cut(s) 1231
StyD4I CCNGG 9 cut(s) 45, 126, 168, 1180, 2408, 2853, 2862, 2971, 2986
StyI CCWWGG 2 cut(s) 371, 1740
TaaI ACNGT 8 cut(s) 1607, 2349, 2424, 2434, 2887, 2940, 3034, 3166
TaiI ACGT 2 cut(s) 350, 1759
TaqI TCGA 7 cut(s) 352, 411, 546, 1119, 1349, 1762, 2759
TaqII GACCGA 1 cut(s) 3070
TauI GCSGC 4 cut(s) 116, 119, 140, 650
TscAI CASTG 2 cut(s) 1238, 1669
TseFI GTSAC 5 cut(s) 122, 164, 417, 1706, 2398
TseI GCWGC 4 cut(s) 1148, 1897, 2700, 2923
Tsp45I GTSAC 5 cut(s) 122, 164, 417, 1706, 2398
TspGWI ACGGA 3 cut(s) 1892, 1977, 2479
TspRI CASTG 2 cut(s) 1238, 1669
Tth111I GACNNNGTC 1 cut(s) 1758
Vha464I CTTAAG 1 cut(s) 769
VpaK11BI GGWCC 4 cut(s) 507, 1138, 1318, 3015
XapI RAATTY 9 cut(s) 101, 905, 932, 1263, 1819, 1919, 1955, 2467, 2982
XbaI TCTAGA 3 cut(s) 1732, 1814, 2137
XceI RCATGY 2 cut(s) 1307, 1801
XmnI GAANNNNTTC 6 cut(s) 613, 1464, 1725, 1920, 2693, 3074
Zsp2I ATGCAT 2 cut(s) 1695, 1852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.