Rmu_sc0002705.1_g000016

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002705.1
OG0000036
Physical Location & Seq
Forward (+)
49571 .. 55148
5578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002705.1_g000016.1.cds

Sequence Viewer

Length: 3930 bp
atggccgatgttagagcagctcatcacaaaacttcatcttccaatactgcatctccttttcccagtaggtatcatgtgttcttgagtttcaggggccaagacactcgcaagacttttaccgatcacctctatacagccttcaaggaccaaggctttcgcaccttcctagatgatgatgaactcgagagaggagaagatattaacccgggacttgagaaagcgattcaacagtcacgaagttctgtcattgtgttctcaaaagattacgcgtcttccagatggtgccttaacgagctgttgatgatccttgaacgcaagaggacctcagatcatgtagttttaccagtcttctacaacgtggaaccatcccaagtgaggaagcagacgggaagttttggggaggcattcgccagacaccaaaagactcaaccgtcggacaaggtacaagaatggaaggacgcaattacgaaagttgcagatcttggagggatggtcttgcagaaccaagatgacgggtacgagtcaaagtttattaaaacaattgttaaagagataggtgacaagcttagtcgtacaccactaagtgttggcttcaacctgattggaatccattctcgagcctacgaaattaatcaatggatacaagatggatcaaatggtgtcggtatacatgtaatttttgggatgtctggaatagggaagacaacagttgcaaaacatgtctataatgcaaacttcagaagattcgaaggaagtagttttatcgaaaatattagagaaacctcagaacaaaccaatggcctagttaaaatacaaaaacaactcctttatgatattttgaatggaagagaagtgaaactacgaaatgctagcgaggggaaaattaagattgaggatgccataagctgtaaaagagttcttcttgttcttgatgacgtggatcatatggaccaatttgatgcagtacttgcgatgcaagatcgattttatccaggaagtaaaatcatcataacaactagccgtgcaacgttattaaaggctcagggagttagtggggtgcattatgttcaagctttggatgatagtgaatcattgaagctctttagttggcatgcttttcgacaagatcatcctattgaaggttacatggaacatactaagaagtttgtacgccacagcggagggcttccattagctcttcaagttttgggttcttctctattcagagaaaatatagatgtttgggagagtgcattaagaaagttggaagcaattccgaatagtgaaatcatgcataaactcagaattagttacgatgcagtagataaacatgatcaggaagtgttcctccacattgcttgtttctttattggaaatgacaaagattacactgttggaatactagatgaatgtgacttgcacacaactattggcattataaatctcattgatagatgtttagtagcaattgacgagaataacaaggtagtgatgcatgacatggttcgtgatatgggaagaggaattgttcgcttagcagcagaggagccccggaaacgtagtagattatggcatcaccaggactctatcaaagtattgagagaaaagaatggtaccaaaagaattgaaggttttgaattaaacttgcaaaggcaccttggagtcactactccttcaactatcccaaacaattttgtcgtcgaaaccagtgcatttgcaaggatgcacaaactaaaactgctccggctcagtcatgtacacctcactggatgctatgaagaatttcctacagaattgagaatgttgtgttggattgcatttcctttggattctattcctattaattttcatttggagaacctagttgttctggaaatgcagtatagtagcttgagacaaatcttgaatggaacaaagtgttttccatccttgaagatcctcgatctcagccattctcatgaccttattgcagctgttgacttctctctatgtctgaatctagagaaactgatacttatagagtgtgaaggcttgattgatgtccatgaatccattggaaagctaggcagacttgtttatttgaatatgaaagattgcagaagtcttaggagactcccaaagaccattggtacgctaaagcgacttgacacacttattctatctggttgtttaaatcttaacaactcttcaattgagatgattaggggcatggagtctctgaaagtgcttgaattagatagaattccaataagtcaattattcactctctgtggggaggtcaaattagagagttacttaccattctctttactatctttaagtctcaaggggtgcaatctttctgatgattcttttcctgtggattttggtagcctatccttactgagaagtttagatctaagtgataatccaatttgtggcctaccaaattgcgtcaagggactgacaatgctagataaactctctttttccaactgtttgagcctcaaatcccttgtagagctaccaaaagtacattttatgttgagtttgggtgattgcaaagcattggtaaaaataacatatcagtcaaggtttgccgccgatacgtcaccataccaatgggataagaaccacagcctagttgagtgggaatacaaatataagttagaacccattggaagagttgatgtagaaatgcttaaccttttgggcttgagaaacttggacttcgaaatcatgccaaccattcggatatgtaaaccatttcaaggttttgaaatatttcatcatggtggaatccagggactgcatgagtatgattgtacagactcttacttcaaaggaaatgcaagaaagatagatatgttcagcacatttcttcctgggaatgtggttccaggccatttcacccataaaagtaaagaaccctcctcaatatcatttatagtgcctttgccttcacttcccggtcttagaattcgaggctttaacatcttcattgtctatgcaatttctgatgatgtcgatgacaaaaattattactggcctagtaatgaacgtgacaagggtgcagtatctattaaagtcgaaaatgagagcaacgatgacaactggcattatacaccagtattccatgctattccagggaagggagaagacatgacatggttaagccattggaatatgaagaatcaagtcaaaggtggggatcaaatggctgtttcattccgggtacaatttagtacatttttgtttctggtaaaagagtggggaatccaggttgtacaggagaagcaagaggataagacgtataccgacctcaacagtaccaccaatgctaattgtaatccgtgtgccactggcggagatatgtcaagctctgatgagccattgccaggaccatacttgctctcctatgaacctcatacagtggaagcagataattgtaatccctgtggcactgatggagatatgtcaagctctgatgagccattgccaggaccatacttgctctcctatgaacctcatacagtggaagcagataaagataaaaaaggagatgaggaagaggcagagggtgatctttttcctgcatcagcaatgaggaccggcagcaataaccgcgagcagggtttcggaaagtggaaggtgcttatcattgccgctgtcatccttgctgctgccatcagtgccgctcatcattttgaggaccctgatctcgacaccaatctatctaaaactgtcactaatccagaagaagaggatggagatcaaacacttggagcagcagctagaactgatggcagtaacaacggcacgcagggactcagaatgcggaagatgctcatcattgccgctgtcttctttattctctctttatctgttcactgcctcttctctttctccggtagagagagatccatgtcgtccattttggtacctacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1309

Amino Acids

147.55

Weight (kDa)

5.94

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1439
AasI GACNNNNNNGTC 1 cut(s) 430
Acc65I GGTACC 2 cut(s) 1613, 3919
AccB1I GGYRCC 4 cut(s) 282, 1613, 1653, 3919
AccB7I CCANNNNNTGG 1 cut(s) 2417
AccBSI CCGCTC 1 cut(s) 3677
AccI GTMKAC 2 cut(s) 667, 3293
AccII CGCG 2 cut(s) 269, 3606
AciI CCGC 8 cut(s) 1179, 2580, 3345, 3604, 3645, 3675, 3817, 3837
AclI AACGTT 1 cut(s) 1028
AclWI GGATC 6 cut(s) 298, 658, 948, 1930, 3198, 3894
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 1781, 2241, 2958
AcuI CTGAAG 1 cut(s) 721
AdeI CACNNNGTG 1 cut(s) 584
AfiI CCNNNNNNNGG 8 cut(s) 375, 1139, 2417, 2750, 3131, 3377, 3479, 3610
AflIII ACRYGT 3 cut(s) 267, 670, 718
AjiI CACGTC 1 cut(s) 937
AjnI CCWGG 9 cut(s) 991, 1578, 2781, 2863, 2878, 3124, 3258, 3376, 3478
AjuI GAANNNNNNNTTGG 2 cut(s) 1792, 1824
Alw26I GTCTC 4 cut(s) 1888, 2104, 2220, 2327
AlwI GGATC 6 cut(s) 298, 658, 948, 1930, 3198, 3894
AlwNI CAGNNNCTG 1 cut(s) 2788
Ama87I CYCGRG 3 cut(s) 182, 205, 615
AoxI GGCC 6 cut(s) 3, 94, 799, 2419, 2881, 3026
ApeKI GCWGC 8 cut(s) 17, 1538, 1970, 3594, 3659, 3662, 3767, 3770
ApoI RAATTY 3 cut(s) 1781, 2241, 2958
AseI ATTAAT 2 cut(s) 630, 1842
Asp700I GAANNNNTTC 4 cut(s) 610, 1272, 1782, 2763
Asp718I GGTACC 2 cut(s) 1613, 3919
AspS9I GGNCC 8 cut(s) 94, 145, 321, 949, 3380, 3482, 3588, 3691
AsuC2I CCSGG 5 cut(s) 206, 207, 1552, 2949, 3212
AsuHPI GGTGA 7 cut(s) 116, 569, 1568, 2546, 2583, 2881, 3572
AsuII TTCGAA 2 cut(s) 747, 2712
AsuNHI GCTAGC 1 cut(s) 869
AvaI CYCGRG 3 cut(s) 182, 205, 615
AvaII GGWCC 7 cut(s) 145, 321, 949, 3380, 3482, 3588, 3691
BanI GGYRCC 4 cut(s) 282, 1613, 1653, 3919
BanII GRGCYC 1 cut(s) 1551
BarI GAAGNNNNNNTAC 2 cut(s) 843, 875
BbsI GAAGAC 5 cut(s) 264, 340, 707, 3144, 3835
BbvI GCAGC 8 cut(s) 29, 1550, 1982, 3606, 3646, 3649, 3779, 3782
BccI CCATC 9 cut(s) 273, 373, 484, 641, 1933, 3440, 3674, 3740, 3776
BceAI ACGGC 2 cut(s) 1005, 3811
BcgI CGANNNNNNTGC 4 cut(s) 1100, 1134, 1691, 1725
BciT130I CCWGG 9 cut(s) 993, 1580, 2783, 2865, 2880, 3126, 3260, 3378, 3480
BciVI GTATCC 1 cut(s) 633
BclI TGATCA 1 cut(s) 1333
BcnI CCSGG 5 cut(s) 206, 207, 1552, 2949, 3212
BcoDI GTCTC 4 cut(s) 1888, 2104, 2220, 2327
BfmI CTRYAG 1 cut(s) 1788
BfuI GTATCC 1 cut(s) 633
BglII AGATCT 2 cut(s) 478, 2395
BlpI GCTNAGC 1 cut(s) 1534
BmcAI AGTACT 1 cut(s) 966
Bme18I GGWCC 7 cut(s) 145, 321, 949, 3380, 3482, 3588, 3691
BmeT110I CYCGRG 3 cut(s) 182, 205, 615
BmgBI CACGTC 1 cut(s) 937
BmgT120I GGNCC 8 cut(s) 94, 145, 321, 949, 3380, 3482, 3588, 3691
BmiI GGNNCC 9 cut(s) 95, 284, 363, 1548, 1615, 1655, 2877, 3693, 3921
BmrI ACTGGG 1 cut(s) 57
BmtI GCTAGC 1 cut(s) 873
BmuI ACTGGG 1 cut(s) 57
BpiI GAAGAC 5 cut(s) 264, 340, 707, 3144, 3835
Bpu10I CCTNAGC 1 cut(s) 1041
Bpu1102I GCTNAGC 1 cut(s) 1534
Bpu14I TTCGAA 2 cut(s) 747, 2712
BpuEI CTTGAG 5 cut(s) 103, 233, 1912, 2309, 2716
BpuMI CCSGG 5 cut(s) 206, 207, 1552, 2949, 3212
Bsa29I ATCGAT 1 cut(s) 982
BsaBI GATNNNNATC 3 cut(s) 605, 3750, 3827
BsaJI CCNNGG 7 cut(s) 148, 205, 1550, 1657, 2782, 2864, 3125
BsaWI WCCGGW 1 cut(s) 3887
BsaXI ACNNNNNCTCC 8 cut(s) 37, 67, 477, 507, 3377, 3407, 3479, 3509
Bsc4I CCNNNNNNNGG 8 cut(s) 375, 1139, 2417, 2750, 3131, 3377, 3479, 3610
Bse118I RCCGGY 1 cut(s) 3590
Bse1I ACTGG 8 cut(s) 63, 344, 1707, 1771, 3029, 3098, 3107, 3346
Bse3DI GCAATG 6 cut(s) 1353, 3371, 3473, 3588, 3639, 3831
Bse8I GATNNNNATC 3 cut(s) 605, 3750, 3827
BseBI CCWGG 9 cut(s) 993, 1580, 2783, 2865, 2880, 3126, 3260, 3378, 3480
BseCI ATCGAT 1 cut(s) 982
BseDI CCNNGG 7 cut(s) 148, 205, 1550, 1657, 2782, 2864, 3125
BseJI GATNNNNATC 3 cut(s) 605, 3750, 3827
BseLI CCNNNNNNNGG 8 cut(s) 375, 1139, 2417, 2750, 3131, 3377, 3479, 3610
BseMI GCAATG 6 cut(s) 1353, 3371, 3473, 3588, 3639, 3831
BseMII CTCAG 8 cut(s) 339, 798, 1055, 1315, 1762, 1960, 2375, 3823
BseNI ACTGG 8 cut(s) 63, 344, 1707, 1771, 3029, 3098, 3107, 3346
BseRI GAGGAG 3 cut(s) 204, 1559, 2902
BseXI GCAGC 8 cut(s) 29, 1550, 1982, 3606, 3646, 3649, 3779, 3782
BsgI GTGCAG 1 cut(s) 3072
Bsh1236I CGCG 2 cut(s) 269, 3606
BshFI GGCC 6 cut(s) 5, 96, 801, 2421, 2883, 3028
BshNI GGYRCC 4 cut(s) 282, 1613, 1653, 3919
BshVI ATCGAT 1 cut(s) 982
BsiHKCI CYCGRG 3 cut(s) 182, 205, 615
BsiSI CCGG 7 cut(s) 206, 1552, 1744, 2949, 3211, 3591, 3888
BslFI GGGAC 4 cut(s) 222, 2454, 2799, 3819
BslI CCNNNNNNNGG 8 cut(s) 375, 1139, 2417, 2750, 3131, 3377, 3479, 3610
BsmAI GTCTC 4 cut(s) 1888, 2104, 2220, 2327
BsmFI GGGAC 4 cut(s) 222, 2454, 2799, 3819
BsmI GAATGC 2 cut(s) 404, 3819
BsnI GGCC 6 cut(s) 5, 96, 801, 2421, 2883, 3028
BsoBI CYCGRG 3 cut(s) 182, 205, 615
Bsp119I TTCGAA 2 cut(s) 747, 2712
Bsp1286I GDGCHC 1 cut(s) 1551
Bsp1407I TGTACA 3 cut(s) 1756, 2804, 3265
Bsp1720I GCTNAGC 1 cut(s) 1534
BspACI CCGC 8 cut(s) 1179, 2580, 3345, 3604, 3645, 3675, 3817, 3837
BspANI GGCC 6 cut(s) 5, 96, 801, 2421, 2883, 3028
BspCNI CTCAG 8 cut(s) 338, 797, 1054, 1314, 1761, 1959, 2376, 3822
BspDI ATCGAT 1 cut(s) 982
BspFNI CGCG 2 cut(s) 269, 3606
BspHI TCATGA 1 cut(s) 1957
BspLI GGNNCC 9 cut(s) 95, 284, 363, 1548, 1615, 1655, 2877, 3693, 3921
BspOI GCTAGC 1 cut(s) 873
BspPI GGATC 6 cut(s) 298, 658, 948, 1930, 3198, 3894
BspQI GCTCTTC 1 cut(s) 1203
BspT104I TTCGAA 2 cut(s) 747, 2712
BspT107I GGYRCC 4 cut(s) 282, 1613, 1653, 3919
BsrBI CCGCTC 1 cut(s) 3677
BsrDI GCAATG 6 cut(s) 1353, 3371, 3473, 3588, 3639, 3831
BsrFI RCCGGY 1 cut(s) 3590
BsrGI TGTACA 3 cut(s) 1756, 2804, 3265
BsrI ACTGG 8 cut(s) 63, 344, 1707, 1771, 3029, 3098, 3107, 3346
BssAI RCCGGY 1 cut(s) 3590
BssECI CCNNGG 7 cut(s) 148, 205, 1550, 1657, 2782, 2864, 3125
BssNAI GTATAC 2 cut(s) 668, 3294
BssT1I CCWWGG 2 cut(s) 148, 1657
Bst1107I GTATAC 2 cut(s) 668, 3294
Bst2UI CCWGG 9 cut(s) 993, 1580, 2783, 2865, 2880, 3126, 3260, 3378, 3480
Bst4CI ACNGT 9 cut(s) 231, 432, 709, 1393, 2477, 3308, 3412, 3514, 3724
Bst6I CTCTTC 8 cut(s) 841, 1203, 1513, 2191, 2656, 3543, 3735, 3881
BstAPI GCANNNNNTGC 1 cut(s) 968
BstAUI TGTACA 3 cut(s) 1756, 2804, 3265
BstBI TTCGAA 2 cut(s) 747, 2712
BstC8I GCNNGC 4 cut(s) 871, 1113, 3608, 3800
BstENI CCTNNNNNAGG 1 cut(s) 1137
BstFNI CGCG 2 cut(s) 269, 3606
BstMAI GTCTC 4 cut(s) 1888, 2104, 2220, 2327
BstMWI GCNNNNNNNGC 4 cut(s) 968, 3603, 3671, 3823
BstNI CCWGG 9 cut(s) 993, 1580, 2783, 2865, 2880, 3126, 3260, 3378, 3480
BstNSI RCATGY 3 cut(s) 674, 722, 1115
BstSFI CTRYAG 1 cut(s) 1788
BstUI CGCG 2 cut(s) 269, 3606
BstV1I GCAGC 8 cut(s) 29, 1550, 1982, 3606, 3646, 3649, 3779, 3782
BstV2I GAAGAC 5 cut(s) 264, 340, 707, 3144, 3835
BstX2I RGATCY 4 cut(s) 478, 1935, 2395, 3899
BstXI CCANNNNNNTGG 1 cut(s) 2601
BstYI RGATCY 4 cut(s) 478, 1935, 2395, 3899
BstZ17I GTATAC 2 cut(s) 668, 3294
Bsu15I ATCGAT 1 cut(s) 982
BsuI GTATCC 1 cut(s) 633
BsuRI GGCC 6 cut(s) 5, 96, 801, 2421, 2883, 3028
BsuTUI ATCGAT 1 cut(s) 982
BtgZI GCGATG 1 cut(s) 986
BtrI CACGTC 1 cut(s) 937
BtsI GCAGTG 1 cut(s) 3868
BtsIMutI CAGTG 9 cut(s) 1389, 1714, 1764, 3339, 3417, 3441, 3519, 3676, 3868
Cac8I GCNNGC 4 cut(s) 871, 1113, 3608, 3800
CaiI CAGNNNCTG 1 cut(s) 2788
CciI TCATGA 1 cut(s) 1957
Cfr10I RCCGGY 1 cut(s) 3590
Cfr13I GGNCC 8 cut(s) 94, 145, 321, 949, 3380, 3482, 3588, 3691
Cfr9I CCCGGG 1 cut(s) 205
ClaI ATCGAT 1 cut(s) 982
CseI GACGC 3 cut(s) 258, 467, 2422
CspCI CAANNNNNGTGG 2 cut(s) 1340, 1375
DraI TTTAAA 1 cut(s) 2172
DraIII CACNNNGTG 1 cut(s) 584
DrdI GACNNNNNNGTC 1 cut(s) 430
DseDI GACNNNNNNGTC 1 cut(s) 430
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 8 cut(s) 841, 1203, 1513, 2191, 2656, 3543, 3735, 3881
EarI CTCTTC 8 cut(s) 841, 1203, 1513, 2191, 2656, 3543, 3735, 3881
EciI GGCGGA 1 cut(s) 3360
Eco130I CCWWGG 2 cut(s) 148, 1657
Eco24I GRGCYC 1 cut(s) 1551
Eco47I GGWCC 7 cut(s) 145, 321, 949, 3380, 3482, 3588, 3691
Eco57I CTGAAG 1 cut(s) 721
Eco88I CYCGRG 3 cut(s) 182, 205, 615
EcoNI CCTNNNNNAGG 1 cut(s) 1137
EcoO109I RGGNCCY 2 cut(s) 321, 3691
EcoRI GAATTC 2 cut(s) 2241, 2958
EcoRII CCWGG 9 cut(s) 991, 1578, 2781, 2863, 2878, 3124, 3258, 3376, 3478
EcoT14I CCWWGG 2 cut(s) 148, 1657
EcoT22I ATGCAT 2 cut(s) 1296, 1497
EcoT38I GRGCYC 1 cut(s) 1551
ErhI CCWWGG 2 cut(s) 148, 1657
FaqI GGGAC 4 cut(s) 222, 2454, 2799, 3819
FauNDI CATATG 1 cut(s) 945
FbaI TGATCA 1 cut(s) 1333
FblI GTMKAC 2 cut(s) 667, 3293
FriOI GRGCYC 1 cut(s) 1551
HaeIII GGCC 6 cut(s) 5, 96, 801, 2421, 2883, 3028
HapII CCGG 7 cut(s) 206, 1552, 1744, 2949, 3211, 3591, 3888
HgaI GACGC 3 cut(s) 258, 467, 2422
HincII GTYRAC 1 cut(s) 1978
HindII GTYRAC 1 cut(s) 1978
HindIII AAGCTT 2 cut(s) 563, 1071
HpaII CCGG 7 cut(s) 206, 1552, 1744, 2949, 3211, 3591, 3888
HphI GGTGA 7 cut(s) 116, 569, 1568, 2546, 2583, 2881, 3572
Hpy166II GTNNAC 7 cut(s) 575, 668, 1759, 1978, 2741, 3294, 3868
Hpy8I GTNNAC 7 cut(s) 575, 668, 1759, 1978, 2741, 3294, 3868
Hpy99I CGWCG 2 cut(s) 436, 1703
HpyCH4III ACNGT 9 cut(s) 231, 432, 709, 1393, 2477, 3308, 3412, 3514, 3724
HpyCH4IV ACGT 7 cut(s) 357, 936, 1028, 1558, 2588, 3040, 3290
HpyF10VI GCNNNNNNNGC 4 cut(s) 968, 3603, 3671, 3823
HpySE526I ACGT 7 cut(s) 357, 936, 1028, 1558, 2588, 3040, 3290
KpnI GGTACC 2 cut(s) 1617, 3923
Ksp22I TGATCA 1 cut(s) 1333
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 3 cut(s) 1546, 1746, 3764
Lsp1109I GCAGC 8 cut(s) 29, 1550, 1982, 3606, 3646, 3649, 3779, 3782
MaeII ACGT 7 cut(s) 357, 936, 1028, 1558, 2588, 3040, 3290
MbiI CCGCTC 1 cut(s) 3677
MfeI CAATTG 3 cut(s) 540, 1467, 2190
MflI RGATCY 4 cut(s) 478, 1935, 2395, 3899
MhlI GDGCHC 1 cut(s) 1551
MluI ACGCGT 1 cut(s) 267
MlyI GAGTC 8 cut(s) 418, 530, 1577, 1671, 2106, 2222, 2804, 3801
MmeI TCCRAC 5 cut(s) 414, 1245, 1375, 1790, 2496
Mph1103I ATGCAT 2 cut(s) 1296, 1497
MroXI GAANNNNTTC 4 cut(s) 610, 1272, 1782, 2763
MslI CAYNNNNRTG 4 cut(s) 1956, 2599, 2772, 2796
MspA1I CMGCKG 4 cut(s) 1179, 1973, 3647, 3839
MspI CCGG 7 cut(s) 206, 1552, 1744, 2949, 3211, 3591, 3888
MunI CAATTG 3 cut(s) 540, 1467, 2190
Mva1269I GAATGC 2 cut(s) 404, 3819
MvaI CCWGG 9 cut(s) 993, 1580, 2783, 2865, 2880, 3126, 3260, 3378, 3480
MvnI CGCG 2 cut(s) 269, 3606
MwoI GCNNNNNNNGC 4 cut(s) 968, 3603, 3671, 3823
NciI CCSGG 5 cut(s) 206, 207, 1552, 2949, 3212
NdeI CATATG 1 cut(s) 945
NheI GCTAGC 1 cut(s) 869
NlaIV GGNNCC 9 cut(s) 95, 284, 363, 1548, 1615, 1655, 2877, 3693, 3921
NmuCI GTSAC 7 cut(s) 231, 557, 1412, 1663, 2589, 3041, 3724
NsiI ATGCAT 2 cut(s) 1296, 1497
NspI RCATGY 3 cut(s) 674, 722, 1115
NspV TTCGAA 2 cut(s) 747, 2712
PaeI GCATGC 1 cut(s) 1115
PaeR7I CTCGAG 2 cut(s) 182, 615
PagI TCATGA 1 cut(s) 1957
PciI ACATGT 2 cut(s) 670, 718
PciSI GCTCTTC 1 cut(s) 1203
PctI GAATGC 2 cut(s) 404, 3819
PdmI GAANNNNTTC 4 cut(s) 610, 1272, 1782, 2763
PflMI CCANNNNNTGG 1 cut(s) 2417
PfoI TCCNGGA 1 cut(s) 991
PleI GAGTC 8 cut(s) 418, 529, 1577, 1670, 2106, 2221, 2804, 3801
PpsI GAGTC 8 cut(s) 418, 529, 1577, 1670, 2106, 2221, 2804, 3801
PpuMI RGGWCCY 2 cut(s) 321, 3691
PscI ACATGT 2 cut(s) 670, 718
PshBI ATTAAT 2 cut(s) 630, 1842
PsiI TTATAA 1 cut(s) 1439
Psp1406I AACGTT 1 cut(s) 1028
Psp5II RGGWCCY 2 cut(s) 321, 3691
Psp6I CCWGG 9 cut(s) 991, 1578, 2781, 2863, 2878, 3124, 3258, 3376, 3478
PspGI CCWGG 9 cut(s) 991, 1578, 2781, 2863, 2878, 3124, 3258, 3376, 3478
PspN4I GGNNCC 9 cut(s) 95, 284, 363, 1548, 1615, 1655, 2877, 3693, 3921
PspPI GGNCC 8 cut(s) 94, 145, 321, 949, 3380, 3482, 3588, 3691
PspPPI RGGWCCY 2 cut(s) 321, 3691
PsrI GAACNNNNNNTAC 2 cut(s) 62, 94
PstNI CAGNNNCTG 1 cut(s) 2788
PsuI RGATCY 4 cut(s) 478, 1935, 2395, 3899
PvuII CAGCTG 1 cut(s) 1973
RseI CAYNNNNRTG 4 cut(s) 1956, 2599, 2772, 2796
SapI GCTCTTC 1 cut(s) 1203
Sau96I GGNCC 8 cut(s) 94, 145, 321, 949, 3380, 3482, 3588, 3691
ScaI AGTACT 1 cut(s) 966
SchI GAGTC 8 cut(s) 418, 530, 1577, 1671, 2106, 2222, 2804, 3801
SduI GDGCHC 1 cut(s) 1551
SfcI CTRYAG 1 cut(s) 1788
Sfr274I CTCGAG 2 cut(s) 182, 615
SfuI TTCGAA 2 cut(s) 747, 2712
SinI GGWCC 7 cut(s) 145, 321, 949, 3380, 3482, 3588, 3691
SlaI CTCGAG 2 cut(s) 182, 615
SmaI CCCGGG 1 cut(s) 207
SmiMI CAYNNNNRTG 4 cut(s) 1956, 2599, 2772, 2796
SmlI CTYRAG 7 cut(s) 82, 182, 212, 615, 1891, 2324, 2695
SmoI CTYRAG 7 cut(s) 82, 182, 212, 615, 1891, 2324, 2695
SphI GCATGC 1 cut(s) 1115
SsiI CCGC 8 cut(s) 1179, 2580, 3345, 3604, 3645, 3675, 3817, 3837
SspI AATATT 2 cut(s) 772, 2763
StyI CCWWGG 2 cut(s) 148, 1657
TaaI ACNGT 9 cut(s) 231, 432, 709, 1393, 2477, 3308, 3412, 3514, 3724
TaiI ACGT 7 cut(s) 360, 939, 1031, 1561, 2591, 3043, 3293
TatI WGTACW 6 cut(s) 964, 1756, 2512, 2804, 3224, 3265
TauI GCSGC 4 cut(s) 2582, 3647, 3677, 3839
TscAI CASTG 9 cut(s) 1396, 1714, 1771, 3346, 3417, 3448, 3519, 3676, 3875
TseFI GTSAC 7 cut(s) 231, 557, 1412, 1663, 2589, 3041, 3724
TseI GCWGC 8 cut(s) 17, 1538, 1970, 3594, 3659, 3662, 3767, 3770
Tsp45I GTSAC 7 cut(s) 231, 557, 1412, 1663, 2589, 3041, 3724
TspGWI ACGGA 1 cut(s) 3321
TspMI CCCGGG 1 cut(s) 205
TspRI CASTG 9 cut(s) 1396, 1714, 1771, 3346, 3417, 3448, 3519, 3676, 3875
Van91I CCANNNNNTGG 1 cut(s) 2417
VpaK11BI GGWCC 7 cut(s) 145, 321, 949, 3380, 3482, 3588, 3691
VspI ATTAAT 2 cut(s) 630, 1842
XagI CCTNNNNNAGG 1 cut(s) 1137
XapI RAATTY 3 cut(s) 1781, 2241, 2958
XbaI TCTAGA 1 cut(s) 1999
XceI RCATGY 3 cut(s) 674, 722, 1115
XcmI CCANNNNNNNNNTGG 1 cut(s) 2051
XhoI CTCGAG 2 cut(s) 182, 615
XmaI CCCGGG 1 cut(s) 205
XmiI GTMKAC 2 cut(s) 667, 3293
XmnI GAANNNNTTC 4 cut(s) 610, 1272, 1782, 2763
ZrmI AGTACT 1 cut(s) 966
Zsp2I ATGCAT 2 cut(s) 1296, 1497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.