MD07G1102200.v1.1

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000036
Physical Location & Seq
Forward (+)
11471896 .. 11476551
4656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1102200.v1.1.491

Sequence Viewer

Length: 3570 bp
ATGGCTCTTGTGAGAACATCTCCAGGAACCTCCTTTGATTCCAACACTTCTCGGGGTTACCGGTACGACGTGTTCTTGAGCTTCAGAGGCGAAGACACTCGCAAGACTTTTACCGACCATCTCTACACAGCCTTGAACAACGCAGGATTTCTCACGGTCCGAGACGACGATGAACTTGAGAGAGGAGAAGATATCAAGCCAGGACTGCAGAAAGCGATCCAGCTGTCACGAGCTTCTGTTGTCGTGTTTTCGAAAGATTACGCGTCATCCAGATGGTGCCTTGATGAGCTTGTGCTGATCCTTGAACGCAGGAGGACCACCTCGGACCATGTAGTTTTACCAGTCTTCTACGATGTCGATCCGTCCCACTTGAGGAAGCAGACGGGAAGTGTTGGAAAAGCATTTGCTAGGCACGAGAAAACTCAGTCGCTGAAAAAGGTGAAGGGATGGAGGGAGGCAGTTGCAGAGGTTGCAGATCTAGCAGGCATCGTCTTACAAAATCAAGCTGATGGGTATGAGTCAAAGTTTATCACAAAAATTGTTAAAGTGGTTGGAGACAAGCTAAGTTGCTCACCATTTAGTGTTGAACCAAAATTGATTGGAATTGAATCTCAAGTCGAAGACATCAATTTGTGGTTACAAGATGGATCAACCAATGTTGGTATACTGGTTGTGTATGGCATGTCTGGAATAGGGAAGACAACCATTGCAAAACATGTTTACAATTCAAACTTTAGAAGCTTTGAAGGAAGTAGTTTCATTGAAAATATCAGAGAAACAGCAGATCAACCAAATGGCTTAGTTCAAATACAAAAGCAACTTCTTGCTGATATTTTGAACGACAGAGAAGTGAAAATACATGGTGTTAGTGAGGGACTAAGAAAGATTGAAAGAGGCATAAGCTCTAGAAGAGTGCTTCTTGTTCTTGATGATGTGGACCATATGGACCAATTAGATGCAGTACTAAAGATAAAAGATCAGTTTTATCCGGGAAGTAAGATACTTATAACAACTAGGCGTGAAAGGTTGTTAAAGGCACATGAAGTTATAAAGGTGCACGAAGTTGAAACTTTGGATTACAATAAATCATTAGAGCTTTTCAGTAGGCATGCTTTTGGCCAGGACCAACCCCTTGAAGGTTACATTGAATATTCAAAAAGGGTTGTAAGTCACTGCGGCGGACTTCCATTAGCTCTTCGAGTTTTAGGTTCTTCTTTATCAGGGGAAAGTATAGATGTATGGGAAAGGGCATTGGAGAAGCTAAAAGTTATCCCTAATGGTGAAATCATGAGTAAACAAAGAATAAGCTATGACAGTTTACAAGATGATCATGACCGGAAATTATTCCTCCACATTGCTTGTTTTCTAATAGGAAGGAACAAAAGCTACATTGTTAGAATACTCGATGGATGTGATTTCTATACAATTGTTGGCATTCAAAATCTCATTGATAGATGCTTGGTGACACTTGATGAATACGGCAAGGTGAAAATGCATGATATGATTCGTGATATGGGAAGAGAAATTGTCCGCTTGGAATCAGAAGAGCTTGAGAAACGTAGTAGATTATGGCACCATAAGGATTCTTTCAGAGTATTGAGGGAAAAGAATGGTACACAAACAATTCAAGGTCTTGCCCTGGATATGCGTATGCATCTTGCAAATAGACCAATAAACACAAATGAGACAGTCTTGGAAACCAATGCATTTGAAAGGATGCGCAATTTACAACTACTCCATCTTAGTCATGTACGGCTGGATGGATGTTATGCAGATTTCCCTACAAGATTAAGATGGTTGTGTTGGCTTCAATTTCCTTTGGATTCTATACCGATTGATTTTCCTTTGGGGTGTCTAATTGTTCTTGAAATGCAATACAGTAGTTTGAGACAAGTTTGGAAAGGAACAAAATTTCTTCCATCATTGAAGTTCCTTGACGTCAGCCATTCCCATGCCCTCACTGAAATCATGGACTTCTCATTTTGCCCCAATCTAGAAGAATTGATTCTTGTTGATTGCACCAGCCTGATCGATGTTCATGAATCCATTGGAAATCTAGAGAGACTCGTGTCCTTGAATATGAAGGATTGCAAGAATCTTAGGATGCTTCCGAAGAATATTTGTTTGCTTAAATCACTTGAAACACTCATTTTATCTGGTTGCTCAAATCTTTATGAGTTTCCATTGGAGATGATAAAGAAGATGGAGTCTCTGAAAGTTCTGAAAACAGATGGTATTCCATTAAGGCCAGAAAGATGTTCAACTAACTTGAGTTCTTTTCCTTCCTCTTTAGTAGAGTTGAATCTCAGTAGGTGCAATCTTTTTGATGATGCCATTCCTACGGATTTAAGTAATCTATCCTCGATACAAAGACTATATCTAGATGAGAATCCAATTTGTAGCCTACCAGTTTTCATCAAAGGTTTGAGGAGGATCGATGAACTCTCTTTTTGTAATTGTAAGAAGCTTGAATCGCTTGTGGGGTTGCCGAGAGTACATGAAAAAATGGACGTATCCGATTGCATATCATTGAAAAAAGTAACATATCAATCGTTTGTGGCGCTCCAACTACAGGCCTTTGGGAATGACAACGACAACATAGTTGAGTGGGAGTACAATTTCAAGTTAGAGCCTATTGATAGAGTTGATGTGGAAATGATCAAACTTTTGGGCTTGTGCAACTTGGGATCCTTGCCACCCATTACAATGAACGCATTTCCATTCTCAAGCTATGGTTCAAAAGTGAGTCCTGTTCAGGGACTGTATCAATATGGTATATTCAGCACATTTTTTGTTGGGAATGAGGTTCCAGGCCGGTTCAGCTATAAAAGTACCAAGTCCTCCATATCTTTTATTGTCCCTTTTCTCCCTTCTAGTCACAGAATCCGAGGCTTGAACATCTTTGCTACCTATGCAAACAAGGAGAATTCTAATTATAATGATGATGATGATGCTGATGATAAACCTCCAATGATAATCAAAGTGAGTAACAAGAGCAAGGGTCTGAAGTGGATCTATTTCCCATTATTCTATGGTATCCCAGGCGACGAAGAAGATATGATATGGTTGAGTCATTGGAAGATGGAGAACGAAACAATATTACAATGTGGAGATGAAGTGGTGGTTTCAATAGTGGCCGGACCACATAAGTTGTTTCGGATAAAGGAGTTTGGTGTTGAGCTTATGCAAGAGTACCAGAATAATATGATTATAAGTACCCAACACAACACCAAATCAGTTCCTAATTATCCAGCTGTCATCGGTGGAGATTTGTCAATGTGGGAAATTATACAAGGAATATACACACTTGGTTTCCTCGGAGAAATTGTAGAAGACACAGATGACTATGAGCAGAGATGGTTCAATCGCCTCATCATGGACAATGATGAAGAAGACACAGACAAAGAAGGGCAAGAGGATGAACCTGATTACACAACTGCAATTACGAAGGCTGCCAGTAACAACTGCGGCCTCAGAGGCTGGAAGGTTCTTGTCACAGCTGCCGGATTAGTGTTCACACTTGCTTTGGTAGTTCGGTCCTCCATCTCCCAGAAAAAGAAGCGACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1190

Amino Acids

135.43

Weight (kDa)

5.92

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 189 2.1e-50 TIR domain
TIR_2 PF13676 24 - 124 3.3e-15 TIR domain
AAA_16 PF13191 199 - 319 6.8e-06 AAA ATPase domain
NB-ARC PF00931 203 - 374 4.6e-22 NB-ARC domain
AAA_22 PF13401 221 - 324 1.2e-06 AAA domain
WHD_ROQ1 PF23282 444 - 513 4.2e-19 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1007, 1049, 2937, 3212
AatII GACGTC 1 cut(s) 1941
Acc16I TGCGCA 1 cut(s) 1721
AccB1I GGYRCC 2 cut(s) 276, 1572
AccI GTMKAC 1 cut(s) 664
AccII CGCG 1 cut(s) 263
AciI CCGC 4 cut(s) 1176, 1179, 1531, 3468
AclWI GGATC 8 cut(s) 211, 292, 353, 655, 2441, 2682, 2695, 3020
AcoI YGGCCR 2 cut(s) 1117, 3135
AcsI RAATTY 2 cut(s) 1910, 2926
AcuI CTGAAG 2 cut(s) 67, 3026
AcyI GRCGYC 1 cut(s) 1938
AfaI GTAC 9 cut(s) 65, 963, 1615, 1752, 2496, 2615, 2833, 3194, 3217
AfiI CCNNNNNNNGG 5 cut(s) 372, 1136, 2572, 2756, 3376
AflIII ACRYGT 3 cut(s) 69, 261, 715
AgeI ACCGGT 1 cut(s) 60
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 6 cut(s) 22, 199, 1119, 1638, 2809, 3040
Alw21I GWGCWC 1 cut(s) 1059
Alw26I GTCTC 6 cut(s) 156, 549, 1679, 1882, 2056, 2214
Alw44I GTGCAC 1 cut(s) 1055
AlwI GGATC 8 cut(s) 211, 292, 353, 655, 2441, 2682, 2695, 3020
AlwNI CAGNNNCTG 3 cut(s) 430, 2761, 3480
Ama87I CYCGRG 1 cut(s) 51
AoxI GGCC 6 cut(s) 1117, 2246, 2574, 2812, 3135, 3469
ApaLI GTGCAC 1 cut(s) 1055
ApeKI GCWGC 2 cut(s) 3452, 3500
ApoI RAATTY 2 cut(s) 1910, 2926
AsiGI ACCGGT 1 cut(s) 60
Asp700I GAANNNNTTC 2 cut(s) 1343, 2004
AspLEI GCGC 2 cut(s) 1722, 2563
AspS9I GGNCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
AsuC2I CCSGG 1 cut(s) 990
AsuHPI GGTGA 5 cut(s) 451, 564, 1292, 1474, 1498
AsuII TTCGAA 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 51
AvaII GGWCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
BaeGI GKGCMC 1 cut(s) 1059
BalI TGGCCA 1 cut(s) 1119
BamHI GGATCC 1 cut(s) 2687
BanI GGYRCC 2 cut(s) 276, 1572
BarI GAAGNNNNNNTAC 2 cut(s) 840, 872
BauI CACGAG 3 cut(s) 228, 413, 2066
BbsI GAAGAC 6 cut(s) 99, 337, 627, 704, 3339, 3399
Bbv12I GWGCWC 1 cut(s) 1059
BbvI GCAGC 2 cut(s) 3439, 3487
BceAI ACGGC 2 cut(s) 1495, 1769
BciT130I CCWGG 6 cut(s) 24, 201, 1121, 1640, 2811, 3042
BciVI GTATCC 2 cut(s) 2524, 3047
BclI TGATCA 2 cut(s) 1327, 2658
BcnI CCSGG 1 cut(s) 990
BcoDI GTCTC 6 cut(s) 156, 549, 1679, 1882, 2056, 2214
BfaI CTAG 8 cut(s) 408, 479, 906, 1014, 1994, 2057, 2381, 2874
BfmI CTRYAG 2 cut(s) 206, 2570
BfoI RGCGCY 1 cut(s) 2564
BfuI GTATCC 2 cut(s) 2524, 3047
BglI GCCNNNNNGGC 1 cut(s) 3477
BglII AGATCT 1 cut(s) 475
BisI GCNGC 4 cut(s) 1177, 3453, 3469, 3501
BlsI GCNGC 4 cut(s) 1178, 3454, 3470, 3502
BmcAI AGTACT 1 cut(s) 963
Bme1390I CCNGG 7 cut(s) 24, 201, 990, 1121, 1640, 2811, 3042
Bme18I GGWCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
BmeT110I CYCGRG 1 cut(s) 51
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
BmiI GGNNCC 5 cut(s) 28, 278, 1574, 2689, 2808
BmrFI CCNGG 7 cut(s) 24, 201, 990, 1121, 1640, 2811, 3042
BmsI GCATC 8 cut(s) 495, 946, 1445, 1663, 1707, 2094, 2320, 2941
BoxI GACNNNNGTC 1 cut(s) 2068
BpiI GAAGAC 6 cut(s) 99, 337, 627, 704, 3339, 3399
BplI GAGNNNNNCTC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 251
BpuEI CTTGAG 7 cut(s) 97, 197, 391, 597, 1571, 2290, 2710
BpuMI CCSGG 1 cut(s) 990
Bsa29I ATCGAT 2 cut(s) 2031, 2436
BsaBI GATNNNNATC 2 cut(s) 357, 2388
BsaHI GRCGYC 1 cut(s) 1938
BsaJI CCNNGG 5 cut(s) 321, 1638, 2887, 3040, 3316
BsaWI WCCGGW 2 cut(s) 60, 1335
BsaXI ACNNNNNCTCC 6 cut(s) 1719, 1749, 3102, 3132, 3312, 3342
Bsc4I CCNNNNNNNGG 5 cut(s) 372, 1136, 2572, 2756, 3376
Bse118I RCCGGY 2 cut(s) 60, 2814
Bse1I ACTGG 4 cut(s) 341, 672, 2408, 3456
Bse3DI GCAATG 2 cut(s) 705, 1353
Bse8I GATNNNNATC 2 cut(s) 357, 2388
BseBI CCWGG 6 cut(s) 24, 201, 1121, 1640, 2811, 3042
BseCI ATCGAT 2 cut(s) 2031, 2436
BseDI CCNNGG 5 cut(s) 321, 1638, 2887, 3040, 3316
BseGI GGATG 8 cut(s) 266, 452, 1415, 1722, 1765, 1769, 2109, 3424
BseJI GATNNNNATC 2 cut(s) 357, 2388
BseLI CCNNNNNNNGG 5 cut(s) 372, 1136, 2572, 2756, 3376
BseMI GCAATG 2 cut(s) 705, 1353
BseMII CTCAG 3 cut(s) 437, 2320, 3487
BseNI ACTGG 4 cut(s) 341, 672, 2408, 3456
BseRI GAGGAG 2 cut(s) 198, 2443
BseSI GKGCMC 1 cut(s) 1059
BseXI GCAGC 2 cut(s) 3439, 3487
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 6 cut(s) 1119, 2248, 2576, 2814, 3137, 3471
BshNI GGYRCC 2 cut(s) 276, 1572
BshTI ACCGGT 1 cut(s) 60
BshVI ATCGAT 2 cut(s) 2031, 2436
BsiHKAI GWGCWC 1 cut(s) 1059
BsiHKCI CYCGRG 1 cut(s) 51
BsiSI CCGG 6 cut(s) 61, 989, 1336, 2815, 3138, 3504
BslFI GGGAC 4 cut(s) 349, 888, 2772, 2843
BslI CCNNNNNNNGG 5 cut(s) 372, 1136, 2572, 2756, 3376
BsmAI GTCTC 6 cut(s) 156, 549, 1679, 1882, 2056, 2214
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 4 cut(s) 349, 888, 2772, 2843
BsmI GAATGC 1 cut(s) 1434
BsnI GGCC 6 cut(s) 1119, 2248, 2576, 2814, 3137, 3471
BsoBI CYCGRG 1 cut(s) 51
Bsp119I TTCGAA 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 1059
BspACI CCGC 4 cut(s) 1176, 1179, 1531, 3468
BspANI GGCC 6 cut(s) 1119, 2248, 2576, 2814, 3137, 3471
BspCNI CTCAG 3 cut(s) 436, 2319, 3486
BspDI ATCGAT 2 cut(s) 2031, 2436
BspFNI CGCG 1 cut(s) 263
BspHI TCATGA 3 cut(s) 1287, 1330, 2038
BspLI GGNNCC 5 cut(s) 28, 278, 1574, 2689, 2808
BspMAI CTGCAG 1 cut(s) 210
BspPI GGATC 8 cut(s) 211, 292, 353, 655, 2441, 2682, 2695, 3020
BspQI GCTCTTC 2 cut(s) 1200, 1539
BspT104I TTCGAA 1 cut(s) 251
BspT107I GGYRCC 2 cut(s) 276, 1572
BsrDI GCAATG 2 cut(s) 705, 1353
BsrFI RCCGGY 2 cut(s) 60, 2814
BsrI ACTGG 4 cut(s) 341, 672, 2408, 3456
BssAI RCCGGY 2 cut(s) 60, 2814
BssECI CCNNGG 5 cut(s) 321, 1638, 2887, 3040, 3316
BssNAI GTATAC 1 cut(s) 665
BssNI GRCGYC 1 cut(s) 1938
BssSI CACGAG 3 cut(s) 228, 413, 2066
Bst1107I GTATAC 1 cut(s) 665
Bst2BI CACGAG 3 cut(s) 228, 413, 2066
Bst2UI CCWGG 6 cut(s) 24, 201, 1121, 1640, 2811, 3042
Bst4CI ACNGT 5 cut(s) 157, 1316, 1690, 1880, 2763
Bst6I CTCTTC 4 cut(s) 904, 1200, 1513, 1539
BstACI GRCGYC 1 cut(s) 1938
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBI TTCGAA 1 cut(s) 251
BstC8I GCNNGC 2 cut(s) 484, 1110
BstDEI CTNAG 8 cut(s) 423, 563, 799, 878, 1742, 2099, 2306, 3473
BstEII GGTNACC 1 cut(s) 56
BstF5I GGATG 8 cut(s) 266, 452, 1415, 1722, 1765, 1769, 2109, 3424
BstFNI CGCG 1 cut(s) 263
BstH2I RGCGCY 1 cut(s) 2564
BstHHI GCGC 2 cut(s) 1722, 2563
BstMAI GTCTC 6 cut(s) 156, 549, 1679, 1882, 2056, 2214
BstMWI GCNNNNNNNGC 8 cut(s) 87, 205, 470, 479, 2473, 2820, 2912, 3477
BstNI CCWGG 6 cut(s) 24, 201, 1121, 1640, 2811, 3042
BstNSI RCATGY 3 cut(s) 685, 719, 1112
BstPAI GACNNNNGTC 1 cut(s) 2068
BstPI GGTNACC 1 cut(s) 56
BstSCI CCNGG 7 cut(s) 22, 199, 988, 1119, 1638, 2809, 3040
BstSFI CTRYAG 2 cut(s) 206, 2570
BstSLI GKGCMC 1 cut(s) 1059
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 2 cut(s) 3439, 3487
BstV2I GAAGAC 6 cut(s) 99, 337, 627, 704, 3339, 3399
BstX2I RGATCY 3 cut(s) 475, 2687, 3012
BstYI RGATCY 3 cut(s) 475, 2687, 3012
BstZ17I GTATAC 1 cut(s) 665
Bsu15I ATCGAT 2 cut(s) 2031, 2436
BsuI GTATCC 2 cut(s) 2524, 3047
BsuRI GGCC 6 cut(s) 1119, 2248, 2576, 2814, 3137, 3471
BsuTUI ATCGAT 2 cut(s) 2031, 2436
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 8 cut(s) 266, 452, 1415, 1722, 1765, 1769, 2109, 3424
BtsI GCAGTG 1 cut(s) 1171
BtsIMutI CAGTG 2 cut(s) 1171, 1959
Cac8I GCNNGC 2 cut(s) 484, 1110
CaiI CAGNNNCTG 3 cut(s) 430, 2761, 3480
CciI TCATGA 3 cut(s) 1287, 1330, 2038
CfoI GCGC 2 cut(s) 1722, 2563
Cfr10I RCCGGY 2 cut(s) 60, 2814
Cfr13I GGNCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
ClaI ATCGAT 2 cut(s) 2031, 2436
CpoI CGGWCCG 1 cut(s) 157
CseI GACGC 1 cut(s) 252
Csp6I GTAC 9 cut(s) 64, 962, 1614, 1751, 2495, 2614, 2832, 3193, 3216
CspAI ACCGGT 1 cut(s) 60
CspCI CAANNNNNGTGG 4 cut(s) 1340, 1375, 3132, 3167
CspI CGGWCCG 1 cut(s) 157
CviQI GTAC 9 cut(s) 64, 962, 1614, 1751, 2495, 2614, 2832, 3193, 3216
DdeI CTNAG 8 cut(s) 423, 563, 799, 878, 1742, 2099, 2306, 3473
EaeI YGGCCR 2 cut(s) 1117, 3135
Eam1104I CTCTTC 4 cut(s) 904, 1200, 1513, 1539
EarI CTCTTC 4 cut(s) 904, 1200, 1513, 1539
EciI GGCGGA 1 cut(s) 1194
Eco147I AGGCCT 1 cut(s) 2576
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
Eco57I CTGAAG 2 cut(s) 67, 3026
Eco88I CYCGRG 1 cut(s) 51
Eco91I GGTNACC 1 cut(s) 56
EcoO65I GGTNACC 1 cut(s) 56
EcoRI GAATTC 1 cut(s) 2926
EcoRII CCWGG 6 cut(s) 22, 199, 1119, 1638, 2809, 3040
EcoRV GATATC 1 cut(s) 193
EcoT22I ATGCAT 3 cut(s) 1497, 1656, 1708
Esp3I CGTCTC 1 cut(s) 156
FaqI GGGAC 4 cut(s) 349, 888, 2772, 2843
FauNDI CATATG 1 cut(s) 942
FbaI TGATCA 2 cut(s) 1327, 2658
FblI GTMKAC 1 cut(s) 664
Fnu4HI GCNGC 4 cut(s) 1177, 3453, 3469, 3501
FokI GGATG 8 cut(s) 253, 459, 1422, 1729, 1772, 1776, 2116, 3431
Fsp4HI GCNGC 4 cut(s) 1177, 3453, 3469, 3501
FspBI CTAG 8 cut(s) 408, 479, 906, 1014, 1994, 2057, 2381, 2874
FspI TGCGCA 1 cut(s) 1721
GlaI GCGC 2 cut(s) 1721, 2562
GluI GCNGC 4 cut(s) 1177, 3453, 3469, 3501
GsuI CTGGAG 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 2564
HaeIII GGCC 6 cut(s) 1119, 2248, 2576, 2814, 3137, 3471
HapII CCGG 6 cut(s) 61, 989, 1336, 2815, 3138, 3504
HgaI GACGC 1 cut(s) 252
HhaI GCGC 2 cut(s) 1722, 2563
Hin1I GRCGYC 1 cut(s) 1938
Hin6I GCGC 2 cut(s) 1720, 2561
HinP1I GCGC 2 cut(s) 1720, 2561
HindIII AAGCTT 2 cut(s) 739, 2465
HpaII CCGG 6 cut(s) 61, 989, 1336, 2815, 3138, 3504
HphI GGTGA 5 cut(s) 451, 564, 1292, 1474, 1498
Hpy166II GTNNAC 8 cut(s) 665, 721, 937, 1057, 1295, 1319, 1616, 3516
Hpy8I GTNNAC 8 cut(s) 665, 721, 937, 1057, 1295, 1319, 1616, 3516
Hpy99I CGWCG 3 cut(s) 71, 170, 3050
HpyCH4III ACNGT 5 cut(s) 157, 1316, 1690, 1880, 2763
HpyCH4IV ACGT 4 cut(s) 69, 1558, 1938, 2511
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 205, 470, 479, 2473, 2820, 2912, 3477
HpyF3I CTNAG 8 cut(s) 423, 563, 799, 878, 1742, 2099, 2306, 3473
HpySE526I ACGT 4 cut(s) 69, 1558, 1938, 2511
Hsp92I GRCGYC 1 cut(s) 1938
HspAI GCGC 2 cut(s) 1720, 2561
Ksp22I TGATCA 2 cut(s) 1327, 2658
LguI GCTCTTC 2 cut(s) 1200, 1539
LmnI GCTCC 1 cut(s) 2568
Lsp1109I GCAGC 2 cut(s) 3439, 3487
LweI GCATC 8 cut(s) 495, 946, 1445, 1663, 1707, 2094, 2320, 2941
MaeI CTAG 8 cut(s) 408, 479, 906, 1014, 1994, 2057, 2381, 2874
MaeII ACGT 4 cut(s) 69, 1558, 1938, 2511
MfeI CAATTG 1 cut(s) 1425
MflI RGATCY 3 cut(s) 475, 2687, 3012
MhlI GDGCHC 1 cut(s) 1059
MlsI TGGCCA 1 cut(s) 1119
MluI ACGCGT 1 cut(s) 261
MluNI TGGCCA 1 cut(s) 1119
MlyI GAGTC 5 cut(s) 527, 2058, 2216, 2755, 3079
MmeI TCCRAC 4 cut(s) 66, 373, 532, 2590
Mox20I TGGCCA 1 cut(s) 1119
Mph1103I ATGCAT 3 cut(s) 1497, 1656, 1708
MroXI GAANNNNTTC 2 cut(s) 1343, 2004
MscI TGGCCA 1 cut(s) 1119
MseI TTAA 6 cut(s) 543, 1031, 1790, 2130, 2243, 2348
MslI CAYNNNNRTG 3 cut(s) 271, 1950, 2705
Msp20I TGGCCA 1 cut(s) 1119
MspA1I CMGCKG 3 cut(s) 223, 3254, 3500
MspI CCGG 6 cut(s) 61, 989, 1336, 2815, 3138, 3504
MspR9I CCNGG 7 cut(s) 24, 201, 990, 1121, 1640, 2811, 3042
MunI CAATTG 1 cut(s) 1425
Mva1269I GAATGC 1 cut(s) 1434
MvaI CCWGG 6 cut(s) 24, 201, 1121, 1640, 2811, 3042
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 8 cut(s) 87, 205, 470, 479, 2473, 2820, 2912, 3477
NciI CCSGG 1 cut(s) 990
NdeI CATATG 1 cut(s) 942
NlaIV GGNNCC 5 cut(s) 28, 278, 1574, 2689, 2808
NmeAIII GCCGAG 1 cut(s) 2514
NmuCI GTSAC 5 cut(s) 225, 1169, 1462, 2876, 3493
NsbI TGCGCA 1 cut(s) 1721
NsiI ATGCAT 3 cut(s) 1497, 1656, 1708
NspI RCATGY 3 cut(s) 685, 719, 1112
NspV TTCGAA 1 cut(s) 251
PaeI GCATGC 1 cut(s) 1112
PagI TCATGA 3 cut(s) 1287, 1330, 2038
PceI AGGCCT 1 cut(s) 2576
PciI ACATGT 1 cut(s) 715
PciSI GCTCTTC 2 cut(s) 1200, 1539
PctI GAATGC 1 cut(s) 1434
PdmI GAANNNNTTC 2 cut(s) 1343, 2004
PfoI TCCNGGA 2 cut(s) 22, 988
PinAI ACCGGT 1 cut(s) 60
PkrI GCNGC 4 cut(s) 1178, 3454, 3470, 3502
PleI GAGTC 5 cut(s) 526, 2058, 2215, 2754, 3078
PpsI GAGTC 5 cut(s) 526, 2058, 2215, 2754, 3078
PscI ACATGT 1 cut(s) 715
PshAI GACNNNNGTC 1 cut(s) 2068
PsiI TTATAA 4 cut(s) 1007, 1049, 2937, 3212
Psp6I CCWGG 6 cut(s) 22, 199, 1119, 1638, 2809, 3040
PspEI GGTNACC 1 cut(s) 56
PspGI CCWGG 6 cut(s) 22, 199, 1119, 1638, 2809, 3040
PspN4I GGNNCC 5 cut(s) 28, 278, 1574, 2689, 2808
PspPI GGNCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
PsrI GAACNNNNNNTAC 4 cut(s) 56, 88, 1370, 1402
PstI CTGCAG 1 cut(s) 210
PstNI CAGNNNCTG 3 cut(s) 430, 2761, 3480
PsuI RGATCY 3 cut(s) 475, 2687, 3012
PvuII CAGCTG 3 cut(s) 223, 3254, 3500
RsaI GTAC 9 cut(s) 65, 963, 1615, 1752, 2496, 2615, 2833, 3194, 3217
RsaNI GTAC 9 cut(s) 64, 962, 1614, 1751, 2495, 2614, 2832, 3193, 3216
RseI CAYNNNNRTG 3 cut(s) 271, 1950, 2705
Rsr2I CGGWCCG 1 cut(s) 157
RsrII CGGWCCG 1 cut(s) 157
SapI GCTCTTC 2 cut(s) 1200, 1539
SaqAI TTAA 6 cut(s) 543, 1031, 1790, 2130, 2243, 2348
SatI GCNGC 4 cut(s) 1177, 3453, 3469, 3501
Sau96I GGNCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
ScaI AGTACT 1 cut(s) 963
SchI GAGTC 5 cut(s) 527, 2058, 2216, 2755, 3079
ScrFI CCNGG 7 cut(s) 24, 201, 990, 1121, 1640, 2811, 3042
SduI GDGCHC 1 cut(s) 1059
SfaNI GCATC 8 cut(s) 495, 946, 1445, 1663, 1707, 2094, 2320, 2941
SfcI CTRYAG 2 cut(s) 206, 2570
SfuI TTCGAA 1 cut(s) 251
SinI GGWCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
SmiMI CAYNNNNRTG 3 cut(s) 271, 1950, 2705
SmlI CTYRAG 7 cut(s) 76, 176, 370, 612, 1550, 2269, 2725
SmoI CTYRAG 7 cut(s) 76, 176, 370, 612, 1550, 2269, 2725
SphI GCATGC 1 cut(s) 1112
SseBI AGGCCT 1 cut(s) 2576
SsiI CCGC 4 cut(s) 1176, 1179, 1531, 3468
SspI AATATT 3 cut(s) 1151, 2119, 3099
SspMI CTAG 8 cut(s) 408, 479, 906, 1014, 1994, 2057, 2381, 2874
StuI AGGCCT 1 cut(s) 2576
StyD4I CCNGG 7 cut(s) 22, 199, 988, 1119, 1638, 2809, 3040
TaaI ACNGT 5 cut(s) 157, 1316, 1690, 1880, 2763
TaiI ACGT 4 cut(s) 72, 1561, 1941, 2514
TaqI TCGA 8 cut(s) 251, 357, 618, 1198, 1404, 2031, 2363, 2436
TaqII GACCGA 1 cut(s) 3525
TatI WGTACW 3 cut(s) 961, 2494, 2613
TauI GCSGC 2 cut(s) 1179, 3471
Tru1I TTAA 6 cut(s) 543, 1031, 1790, 2130, 2243, 2348
Tru9I TTAA 6 cut(s) 543, 1031, 1790, 2130, 2243, 2348
TscAI CASTG 2 cut(s) 1178, 1966
TseFI GTSAC 5 cut(s) 225, 1169, 1462, 2876, 3493
TseI GCWGC 2 cut(s) 3452, 3500
Tsp45I GTSAC 5 cut(s) 225, 1169, 1462, 2876, 3493
TspGWI ACGGA 2 cut(s) 351, 2357
TspRI CASTG 2 cut(s) 1178, 1966
VneI GTGCAC 1 cut(s) 1055
VpaK11BI GGWCC 8 cut(s) 157, 315, 325, 937, 946, 1123, 3140, 3537
XapI RAATTY 2 cut(s) 1910, 2926
XbaI TCTAGA 4 cut(s) 905, 1993, 2056, 2380
XceI RCATGY 3 cut(s) 685, 719, 1112
XmiI GTMKAC 1 cut(s) 664
XmnI GAANNNNTTC 2 cut(s) 1343, 2004
XspI CTAG 8 cut(s) 408, 479, 906, 1014, 1994, 2057, 2381, 2874
ZraI GACGTC 1 cut(s) 1939
ZrmI AGTACT 1 cut(s) 963
Zsp2I ATGCAT 3 cut(s) 1497, 1656, 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.