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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 809 |
| AccB1I | GGYRCC | 1 cut(s) | 291 |
| AccI | GTMKAC | 1 cut(s) | 747 |
| AccII | CGCG | 1 cut(s) | 52 |
| AciI | CCGC | 2 cut(s) | 401, 907 |
| AclI | AACGTT | 1 cut(s) | 1166 |
| AclWI | GGATC | 4 cut(s) | 307, 368, 381, 747 |
| AcoI | YGGCCR | 1 cut(s) | 919 |
| AcsI | RAATTY | 1 cut(s) | 579 |
| AfaI | GTAC | 4 cut(s) | 169, 461, 765, 971 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 142, 711, 938 |
| AflIII | ACRYGT | 1 cut(s) | 84 |
| AgsI | TTSAA | 5 cut(s) | 566, 825, 869, 938, 1085 |
| AjnI | CCWGG | 3 cut(s) | 147, 790, 945 |
| AluBI | AGCT | 8 cut(s) | 220, 304, 452, 558, 898, 995, 1070, 1110 |
| AluI | AGCT | 8 cut(s) | 220, 304, 452, 558, 898, 995, 1070, 1110 |
| Alw21I | GWGCWC | 1 cut(s) | 900 |
| AlwI | GGATC | 4 cut(s) | 307, 368, 381, 747 |
| AoxI | GGCC | 3 cut(s) | 30, 144, 919 |
| ApeKI | GCWGC | 2 cut(s) | 220, 321 |
| ApoI | RAATTY | 1 cut(s) | 579 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1071 |
| AspS9I | GGNCC | 1 cut(s) | 145 |
| AsuHPI | GGTGA | 3 cut(s) | 116, 397, 1037 |
| AsuII | TTCGAA | 1 cut(s) | 266 |
| BalI | TGGCCA | 1 cut(s) | 921 |
| BamHI | GGATCC | 1 cut(s) | 373 |
| BanI | GGYRCC | 1 cut(s) | 291 |
| BanII | GRGCYC | 1 cut(s) | 900 |
| BbsI | GAAGAC | 2 cut(s) | 114, 352 |
| Bbv12I | GWGCWC | 1 cut(s) | 900 |
| BbvI | GCAGC | 2 cut(s) | 207, 308 |
| BccI | CCATC | 4 cut(s) | 282, 387, 427, 449 |
| BceAI | ACGGC | 1 cut(s) | 159 |
| BciT130I | CCWGG | 3 cut(s) | 149, 792, 947 |
| BclI | TGATCA | 1 cut(s) | 340 |
| BfaI | CTAG | 3 cut(s) | 285, 728, 816 |
| BisI | GCNGC | 3 cut(s) | 221, 322, 402 |
| BlsI | GCNGC | 3 cut(s) | 222, 323, 403 |
| BmcAI | AGTACT | 1 cut(s) | 765 |
| Bme1390I | CCNGG | 3 cut(s) | 149, 792, 947 |
| BmgT120I | GGNCC | 1 cut(s) | 145 |
| BmiI | GGNNCC | 2 cut(s) | 293, 375 |
| BmrFI | CCNGG | 3 cut(s) | 149, 792, 947 |
| BmsI | GCATC | 2 cut(s) | 59, 748 |
| BpiI | GAAGAC | 2 cut(s) | 114, 352 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 20, 52 |
| Bpu14I | TTCGAA | 1 cut(s) | 266 |
| BpuEI | CTTGAG | 1 cut(s) | 6 |
| Bsa29I | ATCGAT | 2 cut(s) | 67, 781 |
| BsaBI | GATNNNNATC | 2 cut(s) | 372, 738 |
| BsaJI | CCNNGG | 4 cut(s) | 147, 316, 595, 790 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 142, 711, 938 |
| Bse1I | ACTGG | 1 cut(s) | 360 |
| Bse3DI | GCAATG | 2 cut(s) | 972, 1143 |
| Bse8I | GATNNNNATC | 2 cut(s) | 372, 738 |
| BseBI | CCWGG | 3 cut(s) | 149, 792, 947 |
| BseCI | ATCGAT | 2 cut(s) | 67, 781 |
| BseDI | CCNNGG | 4 cut(s) | 147, 316, 595, 790 |
| BseGI | GGATG | 2 cut(s) | 438, 777 |
| BseJI | GATNNNNATC | 2 cut(s) | 372, 738 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 142, 711, 938 |
| BseMI | GCAATG | 2 cut(s) | 972, 1143 |
| BseMII | CTCAG | 1 cut(s) | 1091 |
| BseNI | ACTGG | 1 cut(s) | 360 |
| BseRI | GAGGAG | 2 cut(s) | 25, 213 |
| BseXI | GCAGC | 2 cut(s) | 207, 308 |
| BseYI | CCCAGC | 1 cut(s) | 428 |
| Bsh1236I | CGCG | 1 cut(s) | 52 |
| BshFI | GGCC | 3 cut(s) | 32, 146, 921 |
| BshNI | GGYRCC | 1 cut(s) | 291 |
| BshVI | ATCGAT | 2 cut(s) | 67, 781 |
| BsiHKAI | GWGCWC | 1 cut(s) | 900 |
| BsiSI | CCGG | 1 cut(s) | 215 |
| BslFI | GGGAC | 1 cut(s) | 191 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 142, 711, 938 |
| BsmFI | GGGAC | 1 cut(s) | 191 |
| BsmI | GAATGC | 1 cut(s) | 555 |
| BsnI | GGCC | 3 cut(s) | 32, 146, 921 |
| Bsp119I | TTCGAA | 1 cut(s) | 266 |
| Bsp1286I | GDGCHC | 1 cut(s) | 900 |
| Bsp143I | GATC | 4 cut(s) | 312, 340, 373, 739 |
| Bsp19I | CCATGG | 1 cut(s) | 595 |
| BspACI | CCGC | 2 cut(s) | 401, 907 |
| BspANI | GGCC | 3 cut(s) | 32, 146, 921 |
| BspCNI | CTCAG | 1 cut(s) | 1092 |
| BspDI | ATCGAT | 2 cut(s) | 67, 781 |
| BspFNI | CGCG | 1 cut(s) | 52 |
| BspHI | TCATGA | 2 cut(s) | 90, 337 |
| BspLI | GGNNCC | 2 cut(s) | 293, 375 |
| BspPI | GGATC | 4 cut(s) | 307, 368, 381, 747 |
| BspQI | GCTCTTC | 1 cut(s) | 905 |
| BspT104I | TTCGAA | 1 cut(s) | 266 |
| BspT107I | GGYRCC | 1 cut(s) | 291 |
| BsrDI | GCAATG | 2 cut(s) | 972, 1143 |
| BsrI | ACTGG | 1 cut(s) | 360 |
| BssECI | CCNNGG | 4 cut(s) | 147, 316, 595, 790 |
| BssMI | GATC | 4 cut(s) | 312, 340, 373, 739 |
| BssT1I | CCWWGG | 2 cut(s) | 316, 595 |
| Bst2UI | CCWGG | 3 cut(s) | 149, 792, 947 |
| Bst6I | CTCTTC | 2 cut(s) | 43, 905 |
| BstBI | TTCGAA | 1 cut(s) | 266 |
| BstDEI | CTNAG | 7 cut(s) | 509, 601, 684, 767, 852, 892, 1100 |
| BstDSI | CCRYGG | 1 cut(s) | 595 |
| BstEII | GGTNACC | 1 cut(s) | 941 |
| BstF5I | GGATG | 2 cut(s) | 438, 777 |
| BstFNI | CGCG | 1 cut(s) | 52 |
| BstKTI | GATC | 4 cut(s) | 315, 343, 376, 742 |
| BstMBI | GATC | 4 cut(s) | 312, 340, 373, 739 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 220, 425, 904 |
| BstNI | CCWGG | 3 cut(s) | 149, 792, 947 |
| BstNSI | RCATGY | 1 cut(s) | 88 |
| BstPI | GGTNACC | 1 cut(s) | 941 |
| BstSCI | CCNGG | 3 cut(s) | 147, 790, 945 |
| BstUI | CGCG | 1 cut(s) | 52 |
| BstV1I | GCAGC | 2 cut(s) | 207, 308 |
| BstV2I | GAAGAC | 2 cut(s) | 114, 352 |
| BstX2I | RGATCY | 1 cut(s) | 373 |
| BstYI | RGATCY | 1 cut(s) | 373 |
| Bsu15I | ATCGAT | 2 cut(s) | 67, 781 |
| BsuRI | GGCC | 3 cut(s) | 32, 146, 921 |
| BsuTUI | ATCGAT | 2 cut(s) | 67, 781 |
| BtgI | CCRYGG | 1 cut(s) | 595 |
| BtsCI | GGATG | 2 cut(s) | 438, 777 |
| BtsI | GCAGTG | 1 cut(s) | 661 |
| BtsIMutI | CAGTG | 1 cut(s) | 661 |
| CciI | TCATGA | 2 cut(s) | 90, 337 |
| Cfr13I | GGNCC | 1 cut(s) | 145 |
| ClaI | ATCGAT | 2 cut(s) | 67, 781 |
| Csp6I | GTAC | 4 cut(s) | 168, 460, 764, 970 |
| CviAII | CATG | 6 cut(s) | 85, 91, 338, 344, 596, 743 |
| CviQI | GTAC | 4 cut(s) | 168, 460, 764, 970 |
| DdeI | CTNAG | 7 cut(s) | 509, 601, 684, 767, 852, 892, 1100 |
| DpnI | GATC | 4 cut(s) | 314, 342, 375, 741 |
| DpnII | GATC | 4 cut(s) | 312, 340, 373, 739 |
| EaeI | YGGCCR | 1 cut(s) | 919 |
| Eam1104I | CTCTTC | 2 cut(s) | 43, 905 |
| EarI | CTCTTC | 2 cut(s) | 43, 905 |
| Ecl136II | GAGCTC | 1 cut(s) | 898 |
| Eco130I | CCWWGG | 2 cut(s) | 316, 595 |
| Eco147I | AGGCCT | 1 cut(s) | 32 |
| Eco24I | GRGCYC | 1 cut(s) | 900 |
| Eco32I | GATATC | 1 cut(s) | 208 |
| Eco53kI | GAGCTC | 1 cut(s) | 898 |
| Eco91I | GGTNACC | 1 cut(s) | 941 |
| EcoICRI | GAGCTC | 1 cut(s) | 898 |
| EcoO65I | GGTNACC | 1 cut(s) | 941 |
| EcoRII | CCWGG | 3 cut(s) | 147, 790, 945 |
| EcoRV | GATATC | 1 cut(s) | 208 |
| EcoT14I | CCWWGG | 2 cut(s) | 316, 595 |
| EcoT38I | GRGCYC | 1 cut(s) | 900 |
| ErhI | CCWWGG | 2 cut(s) | 316, 595 |
| FaeI | CATG | 6 cut(s) | 88, 94, 341, 347, 599, 746 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 702, 734 |
| FaqI | GGGAC | 1 cut(s) | 191 |
| FatI | CATG | 6 cut(s) | 84, 90, 337, 343, 595, 742 |
| FbaI | TGATCA | 1 cut(s) | 340 |
| FblI | GTMKAC | 1 cut(s) | 747 |
| Fnu4HI | GCNGC | 3 cut(s) | 221, 322, 402 |
| FokI | GGATG | 2 cut(s) | 445, 784 |
| FriOI | GRGCYC | 1 cut(s) | 900 |
| Fsp4HI | GCNGC | 3 cut(s) | 221, 322, 402 |
| FspBI | CTAG | 3 cut(s) | 285, 728, 816 |
| GluI | GCNGC | 3 cut(s) | 221, 322, 402 |
| GsaI | CCCAGC | 1 cut(s) | 432 |
| HaeIII | GGCC | 3 cut(s) | 32, 146, 921 |
| HapII | CCGG | 1 cut(s) | 215 |
| Hin1II | CATG | 6 cut(s) | 88, 94, 341, 347, 599, 746 |
| HinfI | GANTC | 2 cut(s) | 464, 887 |
| HpaII | CCGG | 1 cut(s) | 215 |
| HphI | GGTGA | 3 cut(s) | 116, 397, 1037 |
| Hpy166II | GTNNAC | 2 cut(s) | 388, 748 |
| Hpy188I | TCNGA | 3 cut(s) | 101, 575, 958 |
| Hpy188III | TCNNGA | 7 cut(s) | 91, 285, 338, 478, 728, 825, 860 |
| Hpy8I | GTNNAC | 2 cut(s) | 388, 748 |
| HpyAV | CCTTC | 1 cut(s) | 932 |
| HpyCH4IV | ACGT | 5 cut(s) | 170, 820, 864, 1009, 1166 |
| HpyCH4V | TGCA | 9 cut(s) | 72, 158, 223, 324, 419, 516, 553, 761, 1052 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 220, 425, 904 |
| HpyF3I | CTNAG | 7 cut(s) | 509, 601, 684, 767, 852, 892, 1100 |
| HpySE526I | ACGT | 5 cut(s) | 170, 820, 864, 1009, 1166 |
| Hsp92II | CATG | 6 cut(s) | 88, 94, 341, 347, 599, 746 |
| Ksp22I | TGATCA | 1 cut(s) | 340 |
| Kzo9I | GATC | 4 cut(s) | 312, 340, 373, 739 |
| LguI | GCTCTTC | 1 cut(s) | 905 |
| LmnI | GCTCC | 2 cut(s) | 217, 538 |
| Lsp1109I | GCAGC | 2 cut(s) | 207, 308 |
| LweI | GCATC | 2 cut(s) | 59, 748 |
| MaeI | CTAG | 3 cut(s) | 285, 728, 816 |
| MaeII | ACGT | 5 cut(s) | 170, 820, 864, 1009, 1166 |
| MaeIII | GTNAC | 3 cut(s) | 438, 847, 941 |
| MalI | GATC | 4 cut(s) | 314, 342, 375, 741 |
| MboI | GATC | 4 cut(s) | 312, 340, 373, 739 |
| MboII | GAAGA | 9 cut(s) | 30, 51, 119, 215, 352, 704, 710, 892, 1005 |
| MflI | RGATCY | 1 cut(s) | 373 |
| MhlI | GDGCHC | 1 cut(s) | 900 |
| MlsI | TGGCCA | 1 cut(s) | 921 |
| MluCI | AATT | 5 cut(s) | 483, 579, 752, 1086, 1130 |
| MluNI | TGGCCA | 1 cut(s) | 921 |
| MlyI | GAGTC | 1 cut(s) | 473 |
| MmeI | TCCRAC | 2 cut(s) | 259, 1044 |
| Mox20I | TGGCCA | 1 cut(s) | 921 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1071 |
| MscI | TGGCCA | 1 cut(s) | 921 |
| MseI | TTAA | 3 cut(s) | 524, 833, 999 |
| MslI | CAYNNNNRTG | 3 cut(s) | 89, 342, 594 |
| Msp20I | TGGCCA | 1 cut(s) | 921 |
| MspI | CCGG | 1 cut(s) | 215 |
| MspR9I | CCNGG | 3 cut(s) | 149, 792, 947 |
| Mva1269I | GAATGC | 1 cut(s) | 555 |
| MvaI | CCWGG | 3 cut(s) | 149, 792, 947 |
| MvnI | CGCG | 1 cut(s) | 52 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 220, 425, 904 |
| NcoI | CCATGG | 1 cut(s) | 595 |
| NdeII | GATC | 4 cut(s) | 312, 340, 373, 739 |
| NlaIII | CATG | 6 cut(s) | 88, 94, 341, 347, 599, 746 |
| NlaIV | GGNNCC | 2 cut(s) | 293, 375 |
| NspI | RCATGY | 1 cut(s) | 88 |
| NspV | TTCGAA | 1 cut(s) | 266 |
| PagI | TCATGA | 2 cut(s) | 90, 337 |
| PceI | AGGCCT | 1 cut(s) | 32 |
| PciI | ACATGT | 1 cut(s) | 84 |
| PciSI | GCTCTTC | 1 cut(s) | 905 |
| PctI | GAATGC | 1 cut(s) | 555 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1071 |
| PfeI | GAWTC | 1 cut(s) | 887 |
| PkrI | GCNGC | 3 cut(s) | 222, 323, 403 |
| PleI | GAGTC | 1 cut(s) | 472 |
| PpsI | GAGTC | 1 cut(s) | 472 |
| PscI | ACATGT | 1 cut(s) | 84 |
| PsiI | TTATAA | 1 cut(s) | 809 |
| Psp124BI | GAGCTC | 1 cut(s) | 900 |
| Psp1406I | AACGTT | 1 cut(s) | 1166 |
| Psp6I | CCWGG | 3 cut(s) | 147, 790, 945 |
| PspEI | GGTNACC | 1 cut(s) | 941 |
| PspFI | CCCAGC | 1 cut(s) | 428 |
| PspGI | CCWGG | 3 cut(s) | 147, 790, 945 |
| PspN4I | GGNNCC | 2 cut(s) | 293, 375 |
| PspPI | GGNCC | 1 cut(s) | 145 |
| PsuI | RGATCY | 1 cut(s) | 373 |
| RsaI | GTAC | 4 cut(s) | 169, 461, 765, 971 |
| RsaNI | GTAC | 4 cut(s) | 168, 460, 764, 970 |
| RseI | CAYNNNNRTG | 3 cut(s) | 89, 342, 594 |
| SacI | GAGCTC | 1 cut(s) | 900 |
| SapI | GCTCTTC | 1 cut(s) | 905 |
| SaqAI | TTAA | 3 cut(s) | 524, 833, 999 |
| SatI | GCNGC | 3 cut(s) | 221, 322, 402 |
| Sau3AI | GATC | 4 cut(s) | 312, 340, 373, 739 |
| Sau96I | GGNCC | 1 cut(s) | 145 |
| ScaI | AGTACT | 1 cut(s) | 765 |
| SchI | GAGTC | 1 cut(s) | 473 |
| ScrFI | CCNGG | 3 cut(s) | 149, 792, 947 |
| SduI | GDGCHC | 1 cut(s) | 900 |
| SfaNI | GCATC | 2 cut(s) | 59, 748 |
| SfuI | TTCGAA | 1 cut(s) | 266 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 89, 342, 594 |
| SmlI | CTYRAG | 1 cut(s) | 21 |
| SmoI | CTYRAG | 1 cut(s) | 21 |
| Sse9I | AATT | 5 cut(s) | 483, 579, 752, 1086, 1130 |
| SseBI | AGGCCT | 1 cut(s) | 32 |
| SsiI | CCGC | 2 cut(s) | 401, 907 |
| SspI | AATATT | 1 cut(s) | 953 |
| SspMI | CTAG | 3 cut(s) | 285, 728, 816 |
| SstI | GAGCTC | 1 cut(s) | 900 |
| StuI | AGGCCT | 1 cut(s) | 32 |
| StyD4I | CCNGG | 3 cut(s) | 147, 790, 945 |
| StyI | CCWWGG | 2 cut(s) | 316, 595 |
| TaiI | ACGT | 5 cut(s) | 173, 823, 867, 1012, 1169 |
| TaqI | TCGA | 5 cut(s) | 67, 192, 266, 781, 844 |
| TaqII | GACCGA | 1 cut(s) | 1140 |
| TasI | AATT | 5 cut(s) | 483, 579, 752, 1086, 1130 |
| TatI | WGTACW | 1 cut(s) | 763 |
| TauI | GCSGC | 1 cut(s) | 404 |
| TfiI | GAWTC | 1 cut(s) | 887 |
| Tru1I | TTAA | 3 cut(s) | 524, 833, 999 |
| Tru9I | TTAA | 3 cut(s) | 524, 833, 999 |
| TscAI | CASTG | 1 cut(s) | 668 |
| TseI | GCWGC | 2 cut(s) | 220, 321 |
| TspDTI | ATGAA | 5 cut(s) | 79, 201, 788, 900, 1106 |
| TspRI | CASTG | 1 cut(s) | 668 |
| XapI | RAATTY | 1 cut(s) | 579 |
| XbaI | TCTAGA | 2 cut(s) | 284, 727 |
| XceI | RCATGY | 1 cut(s) | 88 |
| XmiI | GTMKAC | 1 cut(s) | 747 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1071 |
| XspI | CTAG | 3 cut(s) | 285, 728, 816 |
| ZrmI | AGTACT | 1 cut(s) | 765 |