Prupe.8G185300_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000036
Physical Location & Seq
Forward (+)
18336603 .. 18342039
5437 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G185300.1

Sequence Viewer

Length: 3672 bp
ATGGCTCTTCCTCCACACAGCTATGACGTGTTCTTGAGTTTCAGAGGCGAAGACACTCGCAAGACTTTTACTGATCATCTCTACACGGCCTTCGTAAATGCAGGCTTTCGCACATTTAGAGATGACGATGAACTCGAAAGAGGAGAAGATATCAAGCCAGAACTGCAGAGAGCCATCCAGCAATCGCGAAGTTCTGTCATTGTGTTTTCAAAAAACTACGCGTCTTCCCGATGGTGCCTTGACGAACTTGTCATGATCCTCGAACGCAAAAGGACGTCAGATCATGTAGTTTTACCAGTCTTCTACGACGTAGACCCATCTCAAGTGAGGAAGCCGACGGCAAGTCTTGCAACAGCTTTTGTAAAAAATGGGTCCTTGAAGGTCAAGGTGGAGAAATGGAGGGCTGCACTTACTGAAGTTGCAGATCTAGCCGGGATGGTCTTGCAAAATCAAGCTGATGGGCACGAGTCAAAATTCATCAAGAAAATTGTTAAGGTGATTGAAGGCAAATTAAGTCGAACTGCATTCAATGTTGCCCCACACCTGATTGGAATCCATTCTCGAGTTCGAGATATTAATTTATGGTTACATGATGGATCAACTAAAGTTAGAGTACTTCTCATTTATGGGATGCGTGGAATAGGGAAGACAACCCTTGCAAAATTCGTTTACAATATAAATTTCAAAAGGTATAAGGGAAGCAGTTTCCTGGAAAATATCAAAGAACATTCAAAGCAAACCAATGGTTTGGTACAAATACAGAAGAAACTTCTTTCTGATGTACTGAATGGCAAAAGAGTGAAAGTAGGGAATATTAGCGAGGGAATAATTAAGATTGAAGATGCTCTTAGCTCTAAGAGGGTTCTTCTCGTTTTCGATGATGTGGATCATGTTGAACAATTAGATGCAGTACTTAGGATGCAAGGCCAGTTTTGTCCAGGAAGCAAAATCATCATCACAACTAGCCACGCAGCTTTGTTAAATGCTAGCCACCAAGCCATTAAGGTGCATAACCTTGAAACTTTTAATAGCAATGAGTCACTGGAGCTCTTCAGTTGGCATGCATTCGGGCAAGACCATCCCGAAAAAGATTACATGGAACTTTCAGAAAGGGTGGTAAACCTTAGCGGAGGACTTCCATTAGCTTTGAAGATTTTGGGTTCTTCTTTGTCAGGGAAGAGTACAGTTGTATGGGAAAGTGCATTAAACAAATTGGAAGCCATTCCAAATGGTGAAATATTAAATAAACTCAGAATAAGCTACGACTCTTTACAAGACCAACATGATCGAAGTTTATTCCTCCATATTGCATGTTTCTTCATCGGAATGGAGAAAGACGTGATTGTTAGAATACTAGATAGTTGTGGCTTCTACACAATTGTTGGCATTCAAAATCTCATTGATAGATGTCTGGTAACAGTTGATGAATATAACAAGGTGCGTATGCATTACATGATCCGGGACATGGGAAGAGGTATTGTTCACCTAGAATCAAAAGAGCCCGGGGAACGTAGTAGATTGTGGAATCATAAAGATTCTTTCAAAGTACTGAAAGAAAAGAATGGTACACAAACAATTGAAGGCCTTGTCCTGAACATGGGAATGCATCCTGCATATTGCACTCCTTCAAGAAATTCGAATGAGGTAACTTTGGAAACCGATGCATTTGCAAGCATGCACAAACTAAGACTACTCCAACTTAGTCATGTACGTCTCATTGGACGCTACAAAGAATTTCCAACAAAACTAAGATGGTTGTGTTGGAATGAGTTTCCATTTGATTATCTACCCAATGATCTTACATTGGAAAGCCTGGTTGTTCTTGAAATGTGCTATAGCAGCTTGAGACAAGTCTGGAAGGGAAAAAAGTATCTTCCATCATTGAAGTTCCTAAACCTCAGTAATTCCCATCGCCTTACCAGTACCCCTGATTTTTCACATGTCCCCAATGTAGAAAGCCTTATTCTTAAAGACTGTACAAACTTGGTTGATGTTGAATCCATTGGAGACCTAAAGAAACTTTTTTATTTGAATATGGAAGATTGCAAAAATATCAGAAAGCTCCCAAAGAACATTTTTATGCTAAAATTCCTCGAAACACTTATCATATCTGGTTGCTCAAGTCTTAATGAGTTTCCAGCAGAGATGGGGAAAATGGAATCTCTGAAAGTGCTTCAAGGAGATGGAGTTCCAATATATCGCTTACTCACTACGATTGTGGAGGTCAAATTACAACCAAGAAAAAATCCAGAGACATATTGGACTAGTTATTTACCATGCAATTTAGTGGAATTAAGTCTTAGTGACTGCAATCTCTCTGATTATGATTTTCCTCGGGGTTTCGGTAACCTGTTCTCATTGCAAAGATTAAATCTAAGCTGTAATCCAATTTCTAGCCTACCAGATTGCATCAGAGGCCTTAAGAGACTCGAGGAACTCTCTTTTTCACAGTGTACGAGACTCGAGTCGCTTCGAGGGCTACCTCCTGTGGCAGAATTGATAGTAAATGGTTGCACATCATTGGAAACAGTAGCATTTCAATCAATGTCGTACCAACCAAAGATCATCTTGGATGAATCCAATTACAAGCTAGTTGAGATTGAGCACTACTTCAAGTTAGAACACATTGAAAGAGTTGATGAAAGAATGATCAACCTTTTGAGCCTGGGCAAGTTGAAATCCACGGAAACCATTATGATGGACAGTACCCTACATGTGTTCAAAACTTGGATGAAGAGTAGGATGCATCCCATCCAGGGACTGAATGAATATGGTATATTCAGCACATTTCTTCCTGGAAATGAGGTTCCAGGCCGTTTCAGCCGTAGAAGTAGTACACAGTCGTCAATATCTTTAACCGTGCCTATACGTGGCCATCTCAAGATTCAGGGCTTGAACGTCTTCTCTGTCTATGCAAAGTCCAACAGTGATTCTCCTAAGAACATCAATGCCAATGTTGAATCAATACCTAACCCACTAGTTACTGCAGTTAAAGTCAGTAATGAAAATGGAAAGAATTTGAAGTGGGTATATGTCCCTTCATTCTTTGCCGTTCCAGGGGATGGGAAAGACATGGTATGGTTAAGCCATTGGAGTTTATTAGGAAGTCAATTATTAGATCGTGGCGATCGGGTGACTGTTTCAGTATTTACGAGATTTGAGTTTCAGGTAAAAGAGTACGGCATTCAGGTTGTGTACGAGCAAGAAAAGAAGATGAGTACTCCAGAAGATAGAATTGACACAAGTCAGTCAAATATGGTGCTCCAAGATGATTATGAGGCGGCAGATGTTATAAGTTTCCCAATAGATGCTCTTTTTTCTCCATCTGTCATTGCTGGAGATTTTTCAGAATCTGATAAGGTGATGTCAGGAACATACTTCCTTAGCAATCGACCTGAAGAAATTGTTGTGTATAGGTGGTTATGGTTCGATGACTTCGTTAGGGACGTTGAGGAAAATATAGGTGAGAGAACAGATGTCGAAAATTTAGAAGAAGAACATGATGGGGACACGAAATTTGGGGACAACGGATCCACCTCCCGTCAAAGAGAGGGAGGGAAGGCATTCAATTTCGGTTGGAATTACGTGGTCAGCATCTTCACGACGACAAAGAAAATTATTTGGAAAGCAACTCCCGACAACTTCCGTTGCGGCGGAACAGAAGGGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1224

Amino Acids

139.25

Weight (kDa)

6.9

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3293
AasI GACNNNNNNGTC 1 cut(s) 248
AatII GACGTC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 234
AccB7I CCANNNNNTGG 1 cut(s) 3062
AccI GTMKAC 1 cut(s) 312
AccII CGCG 2 cut(s) 187, 221
AciI CCGC 4 cut(s) 1128, 3281, 3651, 3654
AclWI GGATC 6 cut(s) 250, 604, 894, 1450, 3525, 3538
AcoI YGGCCR 1 cut(s) 2872
AcsI RAATTY 9 cut(s) 473, 662, 679, 1633, 1733, 2087, 3016, 3484, 3515
AcuI CTGAAG 3 cut(s) 435, 1036, 3417
AcyI GRCGYC 1 cut(s) 275
AfiI CCNNNNNNNGG 6 cut(s) 1465, 1597, 2756, 2870, 3057, 3062
AflII CTTAAG 1 cut(s) 2420
AflIII ACRYGT 4 cut(s) 27, 219, 1939, 2713
AhdI GACNNNNNGTC 1 cut(s) 342
AhlI ACTAGT 2 cut(s) 2264, 2977
AjiI CACGTC 2 cut(s) 28, 1339
AjnI CCWGG 8 cut(s) 708, 937, 1812, 2664, 2754, 2794, 2809, 3055
AloI GAACNNNNNNTCC 4 cut(s) 2172, 2204, 2790, 2822
Alw21I GWGCWC 3 cut(s) 1050, 2607, 3264
Alw26I GTCTC 6 cut(s) 1718, 1840, 2001, 2246, 2419, 2452
AlwI GGATC 6 cut(s) 250, 604, 894, 1450, 3525, 3538
AlwNI CAGNNNCTG 2 cut(s) 2761, 3353
Ama87I CYCGRG 5 cut(s) 561, 1502, 2334, 2429, 2462
AoxI GGCC 6 cut(s) 87, 925, 1582, 2416, 2812, 2872
ApeKI GCWGC 3 cut(s) 404, 971, 1839
ApoI RAATTY 9 cut(s) 473, 662, 679, 1633, 1733, 2087, 3016, 3484, 3515
ArsI GACNNNNNNTTYG 2 cut(s) 3500, 3532
AseI ATTAAT 1 cut(s) 576
Asp700I GAANNNNTTC 4 cut(s) 556, 1221, 2900, 3563
AspS9I GGNCC 1 cut(s) 372
AsuC2I CCSGG 4 cut(s) 433, 1460, 1503, 1504
AsuHPI GGTGA 6 cut(s) 508, 1244, 1475, 3145, 3373, 3476
AsuII TTCGAA 1 cut(s) 1637
AsuNHI GCTAGC 1 cut(s) 986
AvaI CYCGRG 5 cut(s) 561, 1502, 2334, 2429, 2462
AvaII GGWCC 1 cut(s) 372
BaeGI GKGCMC 1 cut(s) 465
BalI TGGCCA 1 cut(s) 2874
BamHI GGATCC 1 cut(s) 3530
BanI GGYRCC 1 cut(s) 234
BanII GRGCYC 2 cut(s) 1050, 1503
BauI CACGAG 1 cut(s) 464
BbsI GAAGAC 5 cut(s) 57, 216, 292, 653, 2893
Bbv12I GWGCWC 3 cut(s) 1050, 2607, 3264
BbvI GCAGC 3 cut(s) 391, 983, 1851
BceAI ACGGC 6 cut(s) 102, 354, 2799, 2808, 3035, 3196
BcgI CGANNNNNNTGC 2 cut(s) 1649, 1683
BciT130I CCWGG 8 cut(s) 710, 939, 1814, 2666, 2756, 2796, 2811, 3057
BclI TGATCA 2 cut(s) 73, 2649
BcnI CCSGG 4 cut(s) 433, 1460, 1503, 1504
BcoDI GTCTC 6 cut(s) 1718, 1840, 2001, 2246, 2419, 2452
BcuI ACTAGT 2 cut(s) 2264, 2977
BfaI CTAG 9 cut(s) 428, 963, 987, 1355, 1487, 2265, 2394, 2591, 2978
BfmI CTRYAG 3 cut(s) 164, 1834, 2985
BfrI CTTAAG 1 cut(s) 2420
BglII AGATCT 1 cut(s) 424
BisI GCNGC 5 cut(s) 405, 972, 1840, 3282, 3652
BlsI GCNGC 5 cut(s) 406, 973, 1841, 3283, 3653
BmcAI AGTACT 4 cut(s) 615, 912, 1548, 3220
Bme18I GGWCC 1 cut(s) 372
BmeRI GACNNNNNGTC 1 cut(s) 342
BmeT110I CYCGRG 5 cut(s) 561, 1502, 2334, 2429, 2462
BmgBI CACGTC 2 cut(s) 28, 1339
BmgT120I GGNCC 1 cut(s) 372
BmiI GGNNCC 4 cut(s) 236, 373, 2808, 3532
BmtI GCTAGC 1 cut(s) 990
BoxI GACNNNNGTC 2 cut(s) 2464, 3243
BpiI GAAGAC 5 cut(s) 57, 216, 292, 653, 2893
BplI GAGNNNNNCTC 4 cut(s) 603, 635, 2423, 2455
BpmI CTGGAG 3 cut(s) 1064, 3207, 3357
Bpu10I CCTNAGC 2 cut(s) 1124, 3383
Bpu14I TTCGAA 1 cut(s) 1637
BpuEI CTTGAG 5 cut(s) 55, 306, 1864, 2104, 2864
BpuMI CCSGG 4 cut(s) 433, 1460, 1503, 1504
BsaAI YACGTR 2 cut(s) 2870, 3586
BsaBI GATNNNNATC 2 cut(s) 551, 885
BsaHI GRCGYC 1 cut(s) 275
BsaI GGTCTC 1 cut(s) 2001
BsaJI CCNNGG 7 cut(s) 1502, 1503, 2333, 2665, 2682, 2755, 3056
BsaXI ACNNNNNCTCC 2 cut(s) 2172, 2202
Bsc4I CCNNNNNNNGG 6 cut(s) 1465, 1597, 2756, 2870, 3057, 3062
Bse1I ACTGG 4 cut(s) 296, 928, 1047, 1920
Bse3DI GCAATG 3 cut(s) 1039, 2357, 3330
Bse8I GATNNNNATC 2 cut(s) 551, 885
BseBI CCWGG 8 cut(s) 710, 939, 1814, 2666, 2756, 2796, 2811, 3057
BseDI CCNNGG 7 cut(s) 1502, 1503, 2333, 2665, 2682, 2755, 3056
BseJI GATNNNNATC 2 cut(s) 551, 885
BseLI CCNNNNNNNGG 6 cut(s) 1465, 1597, 2756, 2870, 3057, 3062
BseMI GCAATG 3 cut(s) 1039, 2357, 3330
BseMII CTCAG 2 cut(s) 1264, 1912
BseNI ACTGG 4 cut(s) 296, 928, 1047, 1920
BseRI GAGGAG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 3 cut(s) 391, 983, 1851
BsgI GTGCAG 1 cut(s) 390
Bsh1236I CGCG 2 cut(s) 187, 221
Bsh1285I CGRYCG 1 cut(s) 3130
BshFI GGCC 6 cut(s) 89, 927, 1584, 2418, 2814, 2874
BshNI GGYRCC 1 cut(s) 234
BsiEI CGRYCG 1 cut(s) 3130
BsiHKAI GWGCWC 3 cut(s) 1050, 2607, 3264
BsiHKCI CYCGRG 5 cut(s) 561, 1502, 2334, 2429, 2462
BsiSI CCGG 3 cut(s) 432, 1459, 1503
BslFI GGGAC 7 cut(s) 1475, 1928, 2772, 3020, 3458, 3521, 3536
BslI CCNNNNNNNGG 6 cut(s) 1465, 1597, 2756, 2870, 3057, 3062
BsmAI GTCTC 6 cut(s) 1718, 1840, 2001, 2246, 2419, 2452
BsmBI CGTCTC 1 cut(s) 1718
BsmFI GGGAC 7 cut(s) 1475, 1928, 2772, 3020, 3458, 3521, 3536
BsmI GAATGC 6 cut(s) 524, 1064, 1386, 1608, 3183, 3563
BsnI GGCC 6 cut(s) 89, 927, 1584, 2418, 2814, 2874
Bso31I GGTCTC 1 cut(s) 2001
BsoBI CYCGRG 5 cut(s) 561, 1502, 2334, 2429, 2462
Bsp119I TTCGAA 1 cut(s) 1637
Bsp1286I GDGCHC 5 cut(s) 465, 1050, 1503, 2607, 3264
Bsp1407I TGTACA 1 cut(s) 1976
Bsp68I TCGCGA 1 cut(s) 187
BspACI CCGC 4 cut(s) 1128, 3281, 3651, 3654
BspANI GGCC 6 cut(s) 89, 927, 1584, 2418, 2814, 2874
BspCNI CTCAG 2 cut(s) 1263, 1911
BspFNI CGCG 2 cut(s) 187, 221
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 236, 373, 2808, 3532
BspMAI CTGCAG 2 cut(s) 168, 2989
BspOI GCTAGC 1 cut(s) 990
BspPI GGATC 6 cut(s) 250, 604, 894, 1450, 3525, 3538
BspQI GCTCTTC 2 cut(s) 12, 1055
BspT104I TTCGAA 1 cut(s) 1637
BspT107I GGYRCC 1 cut(s) 234
BspTI CTTAAG 1 cut(s) 2420
BspTNI GGTCTC 1 cut(s) 2001
BsrDI GCAATG 3 cut(s) 1039, 2357, 3330
BsrGI TGTACA 1 cut(s) 1976
BsrI ACTGG 4 cut(s) 296, 928, 1047, 1920
BssECI CCNNGG 7 cut(s) 1502, 1503, 2333, 2665, 2682, 2755, 3056
BssNI GRCGYC 1 cut(s) 275
BssSI CACGAG 1 cut(s) 464
Bst2BI CACGAG 1 cut(s) 464
Bst2UI CCWGG 8 cut(s) 710, 939, 1814, 2666, 2756, 2796, 2811, 3057
Bst6I CTCTTC 5 cut(s) 12, 1055, 1172, 1465, 2729
BstACI GRCGYC 1 cut(s) 275
BstAFI CTTAAG 1 cut(s) 2420
BstAPI GCANNNNNTGC 1 cut(s) 347
BstAUI TGTACA 1 cut(s) 1976
BstBAI YACGTR 2 cut(s) 2870, 3586
BstBI TTCGAA 1 cut(s) 1637
BstC8I GCNNGC 5 cut(s) 103, 988, 1062, 1672, 1676
BstDSI CCRYGG 1 cut(s) 2682
BstEII GGTNACC 1 cut(s) 2345
BstFNI CGCG 2 cut(s) 187, 221
BstMAI GTCTC 6 cut(s) 1718, 1840, 2001, 2246, 2419, 2452
BstMCI CGRYCG 1 cut(s) 3130
BstMWI GCNNNNNNNGC 7 cut(s) 163, 347, 428, 1839, 2415, 2476, 2820
BstNI CCWGG 8 cut(s) 710, 939, 1814, 2666, 2756, 2796, 2811, 3057
BstNSI RCATGY 5 cut(s) 1064, 1314, 1678, 1943, 2717
BstPAI GACNNNNGTC 2 cut(s) 2464, 3243
BstPI GGTNACC 1 cut(s) 2345
BstSFI CTRYAG 3 cut(s) 164, 1834, 2985
BstSLI GKGCMC 1 cut(s) 465
BstUI CGCG 2 cut(s) 187, 221
BstV1I GCAGC 3 cut(s) 391, 983, 1851
BstV2I GAAGAC 5 cut(s) 57, 216, 292, 653, 2893
BstX2I RGATCY 2 cut(s) 424, 3530
BstXI CCANNNNNNTGG 2 cut(s) 748, 2698
BstYI RGATCY 2 cut(s) 424, 3530
BsuRI GGCC 6 cut(s) 89, 927, 1584, 2418, 2814, 2874
BtgI CCRYGG 1 cut(s) 2682
BtgZI GCGATG 1 cut(s) 1895
BtrI CACGTC 2 cut(s) 28, 1339
BtsIMutI CAGTG 3 cut(s) 1040, 2456, 2932
BtuMI TCGCGA 1 cut(s) 187
Cac8I GCNNGC 5 cut(s) 103, 988, 1062, 1672, 1676
CaiI CAGNNNCTG 2 cut(s) 2761, 3353
CciI TCATGA 1 cut(s) 252
Cfr13I GGNCC 1 cut(s) 372
Cfr9I CCCGGG 1 cut(s) 1502
CseI GACGC 2 cut(s) 210, 1731
DrdI GACNNNNNNGTC 1 cut(s) 248
DriI GACNNNNNGTC 1 cut(s) 342
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 2872
Eam1104I CTCTTC 5 cut(s) 12, 1055, 1172, 1465, 2729
Eam1105I GACNNNNNGTC 1 cut(s) 342
EarI CTCTTC 5 cut(s) 12, 1055, 1172, 1465, 2729
EciI GGCGGA 1 cut(s) 3669
Ecl136II GAGCTC 1 cut(s) 1048
Eco147I AGGCCT 2 cut(s) 1584, 2418
Eco24I GRGCYC 2 cut(s) 1050, 1503
Eco31I GGTCTC 1 cut(s) 2001
Eco32I GATATC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 372
Eco53kI GAGCTC 1 cut(s) 1048
Eco57I CTGAAG 3 cut(s) 435, 1036, 3417
Eco88I CYCGRG 5 cut(s) 561, 1502, 2334, 2429, 2462
Eco91I GGTNACC 1 cut(s) 2345
EcoICRI GAGCTC 1 cut(s) 1048
EcoO109I RGGNCCY 1 cut(s) 372
EcoO65I GGTNACC 1 cut(s) 2345
EcoRII CCWGG 8 cut(s) 708, 937, 1812, 2664, 2754, 2794, 2809, 3055
EcoRV GATATC 1 cut(s) 151
EcoT22I ATGCAT 5 cut(s) 1066, 1449, 1608, 1666, 2748
EcoT38I GRGCYC 2 cut(s) 1050, 1503
Esp3I CGTCTC 1 cut(s) 1718
FalI AAGNNNNNCTT 4 cut(s) 831, 863, 2552, 2584
FaqI GGGAC 7 cut(s) 1475, 1928, 2772, 3020, 3458, 3521, 3536
FbaI TGATCA 2 cut(s) 73, 2649
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 5 cut(s) 405, 972, 1840, 3282, 3652
FriOI GRGCYC 2 cut(s) 1050, 1503
Fsp4HI GCNGC 5 cut(s) 405, 972, 1840, 3282, 3652
FspBI CTAG 9 cut(s) 428, 963, 987, 1355, 1487, 2265, 2394, 2591, 2978
GluI GCNGC 5 cut(s) 405, 972, 1840, 3282, 3652
GsuI CTGGAG 3 cut(s) 1064, 3207, 3357
HaeIII GGCC 6 cut(s) 89, 927, 1584, 2418, 2814, 2874
HapII CCGG 3 cut(s) 432, 1459, 1503
HgaI GACGC 2 cut(s) 210, 1731
Hin1I GRCGYC 1 cut(s) 275
HpaII CCGG 3 cut(s) 432, 1459, 1503
HphI GGTGA 6 cut(s) 508, 1244, 1475, 3145, 3373, 3476
Hpy166II GTNNAC 8 cut(s) 313, 670, 1120, 1483, 1568, 2454, 2837, 3196
Hpy8I GTNNAC 8 cut(s) 313, 670, 1120, 1483, 1568, 2454, 2837, 3196
Hpy99I CGWCG 3 cut(s) 311, 340, 3607
HpyAV CCTTC 9 cut(s) 100, 373, 497, 1574, 1635, 1852, 3048, 3553, 3656
HpyF10VI GCNNNNNNNGC 7 cut(s) 163, 347, 428, 1839, 2415, 2476, 2820
Hsp92I GRCGYC 1 cut(s) 275
Ksp22I TGATCA 2 cut(s) 73, 2649
LguI GCTCTTC 2 cut(s) 12, 1055
LmnI GCTCC 3 cut(s) 1045, 2067, 3267
Lsp1109I GCAGC 3 cut(s) 391, 983, 1851
MaeI CTAG 9 cut(s) 428, 963, 987, 1355, 1487, 2265, 2394, 2591, 2978
MaeIII GTNAC 8 cut(s) 585, 1038, 1414, 1645, 2303, 2345, 2980, 3133
MfeI CAATTG 2 cut(s) 1377, 1575
MflI RGATCY 2 cut(s) 424, 3530
MhlI GDGCHC 5 cut(s) 465, 1050, 1503, 2607, 3264
MlsI TGGCCA 1 cut(s) 2874
MluI ACGCGT 1 cut(s) 219
MluNI TGGCCA 1 cut(s) 2874
MlyI GAGTC 6 cut(s) 476, 1046, 1259, 2421, 2454, 2474
MmeI TCCRAC 5 cut(s) 1720, 1742, 1763, 2946, 3557
Mox20I TGGCCA 1 cut(s) 2874
Mph1103I ATGCAT 5 cut(s) 1066, 1449, 1608, 1666, 2748
MroXI GAANNNNTTC 4 cut(s) 556, 1221, 2900, 3563
MscI TGGCCA 1 cut(s) 2874
MslI CAYNNNNRTG 6 cut(s) 21, 1004, 1325, 1601, 2078, 2696
Msp20I TGGCCA 1 cut(s) 2874
MspCI CTTAAG 1 cut(s) 2420
MspI CCGG 3 cut(s) 432, 1459, 1503
MunI CAATTG 2 cut(s) 1377, 1575
Mva1269I GAATGC 6 cut(s) 524, 1064, 1386, 1608, 3183, 3563
MvaI CCWGG 8 cut(s) 710, 939, 1814, 2666, 2756, 2796, 2811, 3057
MvnI CGCG 2 cut(s) 187, 221
MwoI GCNNNNNNNGC 7 cut(s) 163, 347, 428, 1839, 2415, 2476, 2820
NciI CCSGG 4 cut(s) 433, 1460, 1503, 1504
NheI GCTAGC 1 cut(s) 986
NlaIV GGNNCC 4 cut(s) 236, 373, 2808, 3532
NmuCI GTSAC 3 cut(s) 1038, 2303, 3133
NruI TCGCGA 1 cut(s) 187
NsiI ATGCAT 5 cut(s) 1066, 1449, 1608, 1666, 2748
NspI RCATGY 5 cut(s) 1064, 1314, 1678, 1943, 2717
NspV TTCGAA 1 cut(s) 1637
PaeI GCATGC 2 cut(s) 1064, 1678
PaeR7I CTCGAG 3 cut(s) 561, 2429, 2462
PagI TCATGA 1 cut(s) 252
PceI AGGCCT 2 cut(s) 1584, 2418
PciI ACATGT 2 cut(s) 1939, 2713
PciSI GCTCTTC 2 cut(s) 12, 1055
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 3435, 3444
PctI GAATGC 6 cut(s) 524, 1064, 1386, 1608, 3183, 3563
PdmI GAANNNNTTC 4 cut(s) 556, 1221, 2900, 3563
PflMI CCANNNNNTGG 1 cut(s) 3062
PfoI TCCNGGA 4 cut(s) 708, 937, 1458, 2794
PkrI GCNGC 5 cut(s) 406, 973, 1841, 3283, 3653
Ple19I CGATCG 1 cut(s) 3130
PleI GAGTC 6 cut(s) 475, 1045, 1259, 2421, 2454, 2473
PpsI GAGTC 6 cut(s) 475, 1045, 1259, 2421, 2454, 2473
Ppu21I YACGTR 2 cut(s) 2870, 3586
PpuMI RGGWCCY 1 cut(s) 372
PscI ACATGT 2 cut(s) 1939, 2713
PshAI GACNNNNGTC 2 cut(s) 2464, 3243
PshBI ATTAAT 1 cut(s) 576
PsiI TTATAA 1 cut(s) 3293
Psp124BI GAGCTC 1 cut(s) 1050
Psp5II RGGWCCY 1 cut(s) 372
Psp6I CCWGG 8 cut(s) 708, 937, 1812, 2664, 2754, 2794, 2809, 3055
PspEI GGTNACC 1 cut(s) 2345
PspGI CCWGG 8 cut(s) 708, 937, 1812, 2664, 2754, 2794, 2809, 3055
PspN4I GGNNCC 4 cut(s) 236, 373, 2808, 3532
PspPI GGNCC 1 cut(s) 372
PspPPI RGGWCCY 1 cut(s) 372
PspXI VCTCGAGB 2 cut(s) 2429, 2462
PstI CTGCAG 2 cut(s) 168, 2989
PstNI CAGNNNCTG 2 cut(s) 2761, 3353
PsuI RGATCY 2 cut(s) 424, 3530
PvuI CGATCG 1 cut(s) 3130
RruI TCGCGA 1 cut(s) 187
RseI CAYNNNNRTG 6 cut(s) 21, 1004, 1325, 1601, 2078, 2696
SacI GAGCTC 1 cut(s) 1050
SapI GCTCTTC 2 cut(s) 12, 1055
SatI GCNGC 5 cut(s) 405, 972, 1840, 3282, 3652
Sau96I GGNCC 1 cut(s) 372
ScaI AGTACT 4 cut(s) 615, 912, 1548, 3220
SchI GAGTC 6 cut(s) 476, 1046, 1259, 2421, 2454, 2474
SduI GDGCHC 5 cut(s) 465, 1050, 1503, 2607, 3264
SfcI CTRYAG 3 cut(s) 164, 1834, 2985
Sfr274I CTCGAG 3 cut(s) 561, 2429, 2462
SfuI TTCGAA 1 cut(s) 1637
SinI GGWCC 1 cut(s) 372
SlaI CTCGAG 3 cut(s) 561, 2429, 2462
SmaI CCCGGG 1 cut(s) 1504
SmiMI CAYNNNNRTG 6 cut(s) 21, 1004, 1325, 1601, 2078, 2696
SmlI CTYRAG 9 cut(s) 34, 321, 561, 1843, 2119, 2420, 2429, 2462, 2879
SmoI CTYRAG 9 cut(s) 34, 321, 561, 1843, 2119, 2420, 2429, 2462, 2879
SpeI ACTAGT 2 cut(s) 2264, 2977
SphI GCATGC 2 cut(s) 1064, 1678
SseBI AGGCCT 2 cut(s) 1584, 2418
SsiI CCGC 4 cut(s) 1128, 3281, 3651, 3654
SspI AATATT 2 cut(s) 814, 1239
SspMI CTAG 9 cut(s) 428, 963, 987, 1355, 1487, 2265, 2394, 2591, 2978
SstI GAGCTC 1 cut(s) 1050
StuI AGGCCT 2 cut(s) 1584, 2418
TatI WGTACW 8 cut(s) 613, 781, 910, 1181, 1546, 1976, 2834, 3218
TauI GCSGC 2 cut(s) 3284, 3654
TscAI CASTG 3 cut(s) 1047, 2456, 2932
TseFI GTSAC 3 cut(s) 1038, 2303, 3133
TseI GCWGC 3 cut(s) 404, 971, 1839
Tsp45I GTSAC 3 cut(s) 1038, 2303, 3133
TspGWI ACGGA 3 cut(s) 2699, 3543, 3635
TspMI CCCGGG 1 cut(s) 1502
TspRI CASTG 3 cut(s) 1047, 2456, 2932
Van91I CCANNNNNTGG 1 cut(s) 3062
Vha464I CTTAAG 1 cut(s) 2420
VpaK11BI GGWCC 1 cut(s) 372
VspI ATTAAT 1 cut(s) 576
XapI RAATTY 9 cut(s) 473, 662, 679, 1633, 1733, 2087, 3016, 3484, 3515
XceI RCATGY 5 cut(s) 1064, 1314, 1678, 1943, 2717
XcmI CCANNNNNNNNNTGG 1 cut(s) 2256
XhoI CTCGAG 3 cut(s) 561, 2429, 2462
XmaI CCCGGG 1 cut(s) 1502
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 4 cut(s) 556, 1221, 2900, 3563
XspI CTAG 9 cut(s) 428, 963, 987, 1355, 1487, 2265, 2394, 2591, 2978
ZraI GACGTC 1 cut(s) 276
ZrmI AGTACT 4 cut(s) 615, 912, 1548, 3220
Zsp2I ATGCAT 5 cut(s) 1066, 1449, 1608, 1666, 2748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.