Rw1G016900

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000036
Physical Location & Seq
Forward (+)
37533385 .. 37558133
24749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G016900.1

Sequence Viewer

Length: 3573 bp
ATGGCTGTTGTGAGAGCAGCTTTTCATAGAACTTCCGCTTCCAATAATACTACATCTCCTTTTCCCAGTAGGTATCATGTGTTCTTGAGTTTCAGAGGCCAAGATACTCGCAAAACTTTTACCGACCACCTCCATACAGCTTTCAAGGACCAAGGCTTTCGCACCTTTCTAGATGACGATGAACTTGAGAGAGGAGAAGATATTAACCCGGGACTTGGGAAAGCCATTCGAGACTCGCGAAGTTCTGTCATTGTGTTCTCAAAAGATTACGCTTCTTCCCGGTGGTGCCTTAACGAGCTGGTGATGATCCTTGAACGCAAGAGGACCTCAGATCATGTTGTTTTACCAGTCTTCTACAACGTGGAACCATCCGACGTGAGGAAGCAGACGGGAAGTTTTGGGGAATCGTTTGCTAGACACCAAAAGACTCAACCGCCACACAAGGTGCAGGAATGGAGGGACGCACTTAAAAAAGTTGCAGATCTAGCTGGGATGGTCTTACAGAAACAAACGAATGGGTATGAGGCAACGTTTATTAAAAAAGTTGTTAAAGTGATCGGAGACAAGATAAGTCGCACACCATTAAGCGTTGCCCACAACATAATTGGAATGGAGTCTCGAGTTGAAAGCATTAATTTATGGTTACAAGATCGGTCAACTGATGTTGGCATGCTTTTAATCTACGGCATGTCTGGAATTGGGAAGACAACCATTGCAAAATTTGTTTATAATTTGAACTTCCGTAGGTTTGAAGGAAGCAGCTTCATTGAAAGTATCAGAAAAATTTCAGAAGAACCCAAAGGGTTAGTTGGGATACAATTACAGCTCATTTCCGATATTATGAATGGAAGAGAAGTGAAAATACACAACGTTAGTGAGGGTACCATCATGGTTGGAGATGTCATAAGCTCTAAAAAGATTCTTCTTGTTCTTGATGATGTGGATCATATGGATCAATTAGATGCAGTTCTTAGAATGCAAGATCAGTTTCATCCAGGAAGTAAAATCATCATAACAACTCGGTACAAAAGCCTGTTAAAGGCTCATCACCCTACTAAGTTGCATTATGTAGAAACATTGGGTTACAATGAATCATTAGAGTTGTTCAGTTGGCATGCTTTTGGACAAGACCATCCCATTGAAGGTTATATGGAACTTTCAGAAAAGGTAGTACGTCATGCTGGAGGACTTCCTTTAGCTCTTCAAGTTTTGGGTGCTTCTTTGTCGGGGAAAATTATAGAAGTTTGGGAAAGTGCATTAGAGAAATTACAAGCTATTCCCCATAGAGAAATCGTGCAGAAACTGAGAATAAGCTATGACTCTTTACAAGATGACCATGATCAAGAATTACTCCTCCATATTGCTTGCTTCTTAAGTGGAAAAGACAAAGATGCCATTGTGAAAATACTAAATGGATGTGATTTCTACACAATTGTTGGCATTCAGAATCTCATTGAAAGATGTTTATTAACAATTGACAAACACGACAAGGTGCAGATGCATGACATGATTCGTGACATGGGAAGAGAAATTGTTCGTCAAGAATCAAAAGAGCCCGAGAAACGTAGCAGACTATGGAATCATAAGGATTCTCTCCGAGTGTTGAGAGAAAAGAATGGTACACAAACGATTGAGGGTCTTGTCCTGAACATGCATATGCACACTGCAGACACTCCATCAAAGAATCTCCTCGAGACCCATGCTTTCACAAGGATGTACAAACTAAGACTACTCCATCTTATTCGTGTACAACTCAGTGGATGTTATGAAGAATTCCCAACAGAACTGATATGGTTATGCTGGAATGAATTTCCTTTGGATTCAATACCAATTGATTTTTCTCTAGAGAGTCTGGTTGTTCTTGAAATGCAGCATAGTAACTTGAGACAAGTCTGGAAGGGAACTAAATGTCTTCCATCATTGAAGATCCTTGATCTCAGCTTCTCTCATGGCCTTACCAAAACAGCCGATTTTTCGCTTTGCCCTAATATAGAGAAACTAATTCTTATAGATTGCCGAAGCTTGATTGATGTCAGTTCCATTGGAAACATAAAGAGACTTGTTTACTTGAATATGAGAGACTGCAAAAATCTCCGGGTGCTCCCGGAAAACATTTTTGTCCATTTAGAATTACTTGAAACACTCATCATATCTGGATGCTCAAATCTGAATGAGTTTCCAGTGGAGATGATGAGGAACATGGAATGTTTGAAAGTGCTTGAAACAGATGGAATTCCAATAGATCATATACTGGCTACTCGTGAGGAGTTGAAGTCATGCCCAGGAAAAAGTCCCCTCAGTTTTTGGACCTCTTTACCACCCTCTTTAGTAGCTTTAAGTCTTCGTGGATGCAATCTCTTCGATGATTCGTTTCCTATGGATTTTGGTGGCCTGTCCTCATTGCAAAGATTAGATCTAGGTGACAATCCAATATGTAGCCTACCAAATTGCGTCAAAGGACTTACTGACCTCAAAGAACTCACCTTTTATGACTGCTCGAGACTCATATCTCTTGTAGAGCTACCAAAACTAGATTGTGATTTGAACGTAGAAGGTTGCCTTGCATTGGAAAGGATAACATTTGAATCAGTGAGGAGCCAAGCAAAAATGACCTCAGTGCGTGCCAACAACAGCCTGGTGGAGATTGAGTACAACTTCAGGTTAGTTCCCATTGTCAGAGTTGATGTAGAAATGCTCAACCTCTTGGCCTTGTCCAGCTTGGACTTGGAATCCATGTCAACCATTCGGGTGCACAACCCTTGTGCAGCTCCCTGGCAATATCAGAGTCGTATCCAGGGTGTGTATCAATATGGTATATTCAGCACATATCTTCCTGGGAATGAGGTTCCGGGACAGTTCAGCCACAGAAGAAAAGGGTCATCCTTGATATCAATTACTGTTCCCTTGCCTCTACTTCCTGATCTCAGAATTCGAGGGTTGAACATCTTCATAGTCTATGCAAATGCCGACGGTGAATACTACGCCGGTGATTATGAACCTGATGTGACGATCATTAAAGTCAGAAACAAGAGCAAAGGTTTGAAGTGGATGTATGGCGCAACAATTTACGGCATTCCAGCTGAGGGAGAAGACATGATGTGGTTAAGCCATTGGAATATGGAGAAACAAATACAAGGTGGGGATCAAGTGGTTGTTTCCGTATTTCTACGACGACCCGATTGGTTTAAGATTAAGGAGTGGGGGATCCAGGTTGTGCAGGAAGACCTACACCAACCCAACACCGTCAAAGATCCTCATTATCCACGTGTCATTGGTGGAGATTTGTCAAGACATGAGATGATGCCGGGAACGTACTTGCTGTGTTCTTCAATTGATGAACCGGATCAACTGGAAGAAATCGTTTCGGGTAGGGCGACTTGGTTCAATAACCTCAATAGGGACTCTGATGATGAAGATACAGAAAAAGAAGAGCAAGAGGATGAACCCATGCTTGTACCAACAGGGGGCAGCAGTAATTGTGATTTCAGAGGCTGGAAGCGGCTCATCATTGCTGCTGGATTCTTTCTTTCTCTTTCTCTATTTGTTCGCTTTCCCCTCCCTAGGCGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1190

Amino Acids

135.6

Weight (kDa)

6.15

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 194 4.3e-51 TIR domain
TIR_2 PF13676 27 - 123 7e-16 TIR domain
NB-ARC PF00931 205 - 375 5.8e-18 NB-ARC domain
WHD_ROQ1 PF23282 445 - 516 1.6e-19 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
Acc65I GGTACC 1 cut(s) 881
AccB1I GGYRCC 2 cut(s) 285, 881
AccII CGCG 1 cut(s) 238
AciI CCGC 3 cut(s) 36, 434, 3499
AclI AACGTT 2 cut(s) 530, 870
AclWI GGATC 9 cut(s) 301, 951, 960, 1921, 3150, 3199, 3212, 3245, 3351
AcsI RAATTY 6 cut(s) 719, 783, 1772, 1808, 2232, 2928
AcuI CTGAAG 1 cut(s) 2641
AcvI CACGTG 1 cut(s) 3266
AdeI CACNNNGTG 1 cut(s) 445
AfaI GTAC 9 cut(s) 883, 1025, 1173, 1621, 1718, 1749, 2651, 3314, 3456
AfiI CCNNNNNNNGG 9 cut(s) 215, 378, 1039, 1142, 2566, 3083, 3397, 3464, 3561
AflII CTTAAG 1 cut(s) 1372
AflIII ACRYGT 1 cut(s) 3265
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 7 cut(s) 994, 2281, 2634, 2771, 2793, 2833, 3207
AjuI GAANNNNNNNTTGG 2 cut(s) 1822, 1854
Alw21I GWGCWC 2 cut(s) 2103, 2754
Alw26I GTCTC 8 cut(s) 225, 555, 621, 1688, 1879, 2050, 2073, 2494
Alw44I GTGCAC 1 cut(s) 2750
AlwI GGATC 9 cut(s) 301, 951, 960, 1921, 3150, 3199, 3212, 3245, 3351
AlwNI CAGNNNCTG 2 cut(s) 1303, 3492
Ama87I CYCGRG 5 cut(s) 208, 618, 1556, 1691, 2497
AoxI GGCC 4 cut(s) 97, 1951, 2389, 2706
ApaLI GTGCAC 1 cut(s) 2750
ApeKI GCWGC 6 cut(s) 17, 759, 1870, 2765, 3468, 3512
ApoI RAATTY 6 cut(s) 719, 783, 1772, 1808, 2232, 2928
AseI ATTAAT 1 cut(s) 633
Asp700I GAANNNNTTC 3 cut(s) 784, 1533, 2945
Asp718I GGTACC 1 cut(s) 881
AspA2I CCTAGG 1 cut(s) 3560
AspLEI GCGC 1 cut(s) 3059
AspS9I GGNCC 3 cut(s) 148, 324, 2307
AsuC2I CCSGG 7 cut(s) 209, 210, 280, 2096, 2105, 2850, 3306
AsuHPI GGTGA 6 cut(s) 313, 1040, 2432, 2473, 2984, 2999
AvaI CYCGRG 5 cut(s) 208, 618, 1556, 1691, 2497
AvaII GGWCC 3 cut(s) 148, 324, 2307
AvrII CCTAGG 1 cut(s) 3560
BaeGI GKGCMC 1 cut(s) 2754
BamHI GGATCC 1 cut(s) 3204
BanI GGYRCC 2 cut(s) 285, 881
BanII GRGCYC 1 cut(s) 1557
BarI GAAGNNNNNNTAC 2 cut(s) 846, 878
BauI CACGAG 1 cut(s) 2259
BbrPI CACGTG 1 cut(s) 3266
BbsI GAAGAC 6 cut(s) 343, 710, 1904, 2333, 3096, 3228
Bbv12I GWGCWC 2 cut(s) 2103, 2754
BbvCI CCTCAGC 1 cut(s) 3081
BbvI GCAGC 6 cut(s) 29, 771, 1882, 2777, 3480, 3499
BccI CCATC 8 cut(s) 376, 487, 893, 1140, 1684, 1743, 1924, 2222
BceAI ACGGC 2 cut(s) 700, 3085
BcgI CGANNNNNNTGC 4 cut(s) 1682, 1716, 2341, 2375
BciT130I CCWGG 7 cut(s) 996, 2283, 2636, 2773, 2795, 2835, 3209
BciVI GTATCC 2 cut(s) 807, 2801
BclI TGATCA 1 cut(s) 1339
BcnI CCSGG 7 cut(s) 209, 210, 280, 2096, 2105, 2850, 3306
BcoDI GTCTC 8 cut(s) 225, 555, 621, 1688, 1879, 2050, 2073, 2494
BfaI CTAG 7 cut(s) 170, 414, 485, 1844, 2417, 2531, 3561
BfmI CTRYAG 1 cut(s) 1665
BfrI CTTAAG 1 cut(s) 1372
BfuI GTATCC 2 cut(s) 807, 2801
BglII AGATCT 2 cut(s) 481, 2413
BisI GCNGC 7 cut(s) 18, 760, 1871, 2766, 3469, 3500, 3513
BlnI CCTAGG 1 cut(s) 3560
BlsI GCNGC 7 cut(s) 19, 761, 1872, 2767, 3470, 3501, 3514
Bme18I GGWCC 3 cut(s) 148, 324, 2307
BmeT110I CYCGRG 5 cut(s) 208, 618, 1556, 1691, 2497
BmgBI CACGTC 1 cut(s) 376
BmgT120I GGNCC 3 cut(s) 148, 324, 2307
BmiI GGNNCC 6 cut(s) 287, 366, 883, 2597, 2847, 3206
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 6 cut(s) 952, 1381, 1488, 2147, 2339, 3291
BmuI ACTGGG 1 cut(s) 60
BpiI GAAGAC 6 cut(s) 343, 710, 1904, 2333, 3096, 3228
BpmI CTGGAG 1 cut(s) 1203
Bpu10I CCTNAGC 1 cut(s) 3081
BpuEI CTTGAG 3 cut(s) 106, 206, 1903
BpuMI CCSGG 7 cut(s) 209, 210, 280, 2096, 2105, 2850, 3306
BsaAI YACGTR 1 cut(s) 3266
BsaBI GATNNNNATC 1 cut(s) 942
BsaI GGTCTC 1 cut(s) 1688
BsaJI CCNNGG 7 cut(s) 151, 208, 2281, 2771, 2794, 2834, 3560
BsaWI WCCGGW 1 cut(s) 3340
BsaXI ACNNNNNCTCC 6 cut(s) 40, 70, 2258, 2288, 2633, 2663
Bsc4I CCNNNNNNNGG 9 cut(s) 215, 378, 1039, 1142, 2566, 3083, 3397, 3464, 3561
Bse118I RCCGGY 1 cut(s) 2984
Bse1I ACTGG 5 cut(s) 66, 347, 2180, 2256, 3354
Bse3DI GCAATG 3 cut(s) 711, 2399, 3507
Bse8I GATNNNNATC 1 cut(s) 942
BseBI CCWGG 7 cut(s) 996, 2283, 2636, 2773, 2795, 2835, 3209
BseDI CCNNGG 7 cut(s) 151, 208, 2281, 2771, 2794, 2834, 3560
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 9 cut(s) 215, 378, 1039, 1142, 2566, 3083, 3397, 3464, 3561
BseMI GCAATG 3 cut(s) 711, 2399, 3507
BseMII CTCAG 8 cut(s) 342, 1295, 1768, 1951, 2311, 2628, 2938, 3072
BseNI ACTGG 5 cut(s) 66, 347, 2180, 2256, 3354
BseRI GAGGAG 5 cut(s) 207, 1343, 1679, 2279, 2608
BseSI GKGCMC 1 cut(s) 2754
BseXI GCAGC 6 cut(s) 29, 771, 1882, 2777, 3480, 3499
BseYI CCCAGC 1 cut(s) 488
BsgI GTGCAG 5 cut(s) 467, 1316, 1514, 2784, 3236
Bsh1236I CGCG 1 cut(s) 238
BshFI GGCC 4 cut(s) 99, 1953, 2391, 2708
BshNI GGYRCC 2 cut(s) 285, 881
BsiHKAI GWGCWC 2 cut(s) 2103, 2754
BsiHKCI CYCGRG 5 cut(s) 208, 618, 1556, 1691, 2497
BsiSI CCGG 8 cut(s) 209, 280, 2095, 2105, 2849, 2985, 3305, 3341
BslFI GGGAC 5 cut(s) 225, 473, 2277, 2865, 3413
BslI CCNNNNNNNGG 9 cut(s) 215, 378, 1039, 1142, 2566, 3083, 3397, 3464, 3561
BsmAI GTCTC 8 cut(s) 225, 555, 621, 1688, 1879, 2050, 2073, 2494
BsmFI GGGAC 5 cut(s) 225, 473, 2277, 2865, 3413
BsmI GAATGC 3 cut(s) 981, 1440, 3072
BsnI GGCC 4 cut(s) 99, 1953, 2391, 2708
Bso31I GGTCTC 1 cut(s) 1688
BsoBI CYCGRG 5 cut(s) 208, 618, 1556, 1691, 2497
Bsp1286I GDGCHC 3 cut(s) 1557, 2103, 2754
Bsp1407I TGTACA 2 cut(s) 1716, 1747
Bsp68I TCGCGA 1 cut(s) 238
BspACI CCGC 3 cut(s) 36, 434, 3499
BspANI GGCC 4 cut(s) 99, 1953, 2391, 2708
BspCNI CTCAG 8 cut(s) 341, 1296, 1767, 1950, 2310, 2627, 2937, 3073
BspFNI CGCG 1 cut(s) 238
BspLI GGNNCC 6 cut(s) 287, 366, 883, 2597, 2847, 3206
BspMAI CTGCAG 1 cut(s) 1669
BspPI GGATC 9 cut(s) 301, 951, 960, 1921, 3150, 3199, 3212, 3245, 3351
BspQI GCTCTTC 2 cut(s) 1206, 3423
BspT107I GGYRCC 2 cut(s) 285, 881
BspTI CTTAAG 1 cut(s) 1372
BspTNI GGTCTC 1 cut(s) 1688
BsrDI GCAATG 3 cut(s) 711, 2399, 3507
BsrFI RCCGGY 1 cut(s) 2984
BsrGI TGTACA 2 cut(s) 1716, 1747
BsrI ACTGG 5 cut(s) 66, 347, 2180, 2256, 3354
BssAI RCCGGY 1 cut(s) 2984
BssECI CCNNGG 7 cut(s) 151, 208, 2281, 2771, 2794, 2834, 3560
BssSI CACGAG 1 cut(s) 2259
BssT1I CCWWGG 2 cut(s) 151, 3560
Bst2BI CACGAG 1 cut(s) 2259
Bst2UI CCWGG 7 cut(s) 996, 2283, 2636, 2773, 2795, 2835, 3209
Bst4CI ACNGT 4 cut(s) 2856, 2899, 2972, 3244
Bst6I CTCTTC 5 cut(s) 844, 1206, 1519, 2363, 3423
BstAFI CTTAAG 1 cut(s) 1372
BstAUI TGTACA 2 cut(s) 1716, 1747
BstBAI YACGTR 1 cut(s) 3266
BstC8I GCNNGC 4 cut(s) 671, 1116, 1366, 2622
BstENI CCTNNNNNAGG 1 cut(s) 1037
BstFNI CGCG 1 cut(s) 238
BstHHI GCGC 1 cut(s) 3059
BstMAI GTCTC 8 cut(s) 225, 555, 621, 1688, 1879, 2050, 2073, 2494
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNI CCWGG 7 cut(s) 996, 2283, 2636, 2773, 2795, 2835, 3209
BstNSI RCATGY 4 cut(s) 673, 691, 1118, 1654
BstSFI CTRYAG 1 cut(s) 1665
BstSLI GKGCMC 1 cut(s) 2754
BstUI CGCG 1 cut(s) 238
BstV1I GCAGC 6 cut(s) 29, 771, 1882, 2777, 3480, 3499
BstV2I GAAGAC 6 cut(s) 343, 710, 1904, 2333, 3096, 3228
BstX2I RGATCY 5 cut(s) 481, 1926, 2413, 3204, 3250
BstYI RGATCY 5 cut(s) 481, 1926, 2413, 3204, 3250
BsuI GTATCC 2 cut(s) 807, 2801
BsuRI GGCC 4 cut(s) 99, 1953, 2391, 2708
BtrI CACGTC 1 cut(s) 376
BtsI GCAGTG 1 cut(s) 1662
BtsIMutI CAGTG 5 cut(s) 1662, 1762, 2187, 2595, 2622
BtuMI TCGCGA 1 cut(s) 238
Cac8I GCNNGC 4 cut(s) 671, 1116, 1366, 2622
CaiI CAGNNNCTG 2 cut(s) 1303, 3492
CfoI GCGC 1 cut(s) 3059
Cfr10I RCCGGY 1 cut(s) 2984
Cfr13I GGNCC 3 cut(s) 148, 324, 2307
Cfr9I CCCGGG 1 cut(s) 208
CseI GACGC 2 cut(s) 470, 2440
Csp6I GTAC 9 cut(s) 882, 1024, 1172, 1620, 1717, 1748, 2650, 3313, 3455
CviQI GTAC 9 cut(s) 882, 1024, 1172, 1620, 1717, 1748, 2650, 3313, 3455
DraIII CACNNNGTG 1 cut(s) 445
Eam1104I CTCTTC 5 cut(s) 844, 1206, 1519, 2363, 3423
EarI CTCTTC 5 cut(s) 844, 1206, 1519, 2363, 3423
Eco130I CCWWGG 2 cut(s) 151, 3560
Eco24I GRGCYC 1 cut(s) 1557
Eco31I GGTCTC 1 cut(s) 1688
Eco32I GATATC 1 cut(s) 2889
Eco47I GGWCC 3 cut(s) 148, 324, 2307
Eco57I CTGAAG 1 cut(s) 2641
Eco72I CACGTG 1 cut(s) 3266
Eco88I CYCGRG 5 cut(s) 208, 618, 1556, 1691, 2497
EcoNI CCTNNNNNAGG 1 cut(s) 1037
EcoO109I RGGNCCY 1 cut(s) 324
EcoRI GAATTC 3 cut(s) 1772, 2232, 2928
EcoRII CCWGG 7 cut(s) 994, 2281, 2634, 2771, 2793, 2833, 3207
EcoRV GATATC 1 cut(s) 2889
EcoT14I CCWWGG 2 cut(s) 151, 3560
EcoT22I ATGCAT 2 cut(s) 1503, 1656
EcoT38I GRGCYC 1 cut(s) 1557
ErhI CCWWGG 2 cut(s) 151, 3560
FalI AAGNNNNNCTT 2 cut(s) 2544, 2576
FaqI GGGAC 5 cut(s) 225, 473, 2277, 2865, 3413
FauNDI CATATG 2 cut(s) 948, 1656
FbaI TGATCA 1 cut(s) 1339
Fnu4HI GCNGC 7 cut(s) 18, 760, 1871, 2766, 3469, 3500, 3513
FriOI GRGCYC 1 cut(s) 1557
Fsp4HI GCNGC 7 cut(s) 18, 760, 1871, 2766, 3469, 3500, 3513
FspBI CTAG 7 cut(s) 170, 414, 485, 1844, 2417, 2531, 3561
GlaI GCGC 1 cut(s) 3058
GluI GCNGC 7 cut(s) 18, 760, 1871, 2766, 3469, 3500, 3513
GsaI CCCAGC 1 cut(s) 492
GsuI CTGGAG 1 cut(s) 1203
HaeIII GGCC 4 cut(s) 99, 1953, 2391, 2708
HapII CCGG 8 cut(s) 209, 280, 2095, 2105, 2849, 2985, 3305, 3341
HgaI GACGC 2 cut(s) 470, 2440
HhaI GCGC 1 cut(s) 3059
Hin6I GCGC 1 cut(s) 3057
HinP1I GCGC 1 cut(s) 3057
HincII GTYRAC 2 cut(s) 657, 2739
HindII GTYRAC 2 cut(s) 657, 2739
HindIII AAGCTT 1 cut(s) 2020
HpaII CCGG 8 cut(s) 209, 280, 2095, 2105, 2849, 2985, 3305, 3341
HphI GGTGA 6 cut(s) 313, 1040, 2432, 2473, 2984, 2999
Hpy166II GTNNAC 6 cut(s) 657, 1622, 1748, 2065, 2739, 2752
Hpy8I GTNNAC 6 cut(s) 657, 1622, 1748, 2065, 2739, 2752
Hpy99I CGWCG 3 cut(s) 377, 2972, 3174
HpyAV CCTTC 4 cut(s) 746, 1136, 1891, 2546
HpyCH4III ACNGT 4 cut(s) 2856, 2899, 2972, 3244
HpyCH4IV ACGT 9 cut(s) 360, 375, 530, 870, 1174, 1564, 2547, 3265, 3311
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpySE526I ACGT 9 cut(s) 360, 375, 530, 870, 1174, 1564, 2547, 3265, 3311
HspAI GCGC 1 cut(s) 3057
KpnI GGTACC 1 cut(s) 885
Ksp22I TGATCA 1 cut(s) 1339
LguI GCTCTTC 2 cut(s) 1206, 3423
LmnI GCTCC 3 cut(s) 2106, 2595, 2773
Lsp1109I GCAGC 6 cut(s) 29, 771, 1882, 2777, 3480, 3499
LweI GCATC 6 cut(s) 952, 1381, 1488, 2147, 2339, 3291
MaeI CTAG 7 cut(s) 170, 414, 485, 1844, 2417, 2531, 3561
MaeII ACGT 9 cut(s) 360, 375, 530, 870, 1174, 1564, 2547, 3265, 3311
MaeIII GTNAC 6 cut(s) 642, 1082, 1514, 1877, 2420, 3004
MfeI CAATTG 4 cut(s) 1431, 1473, 1830, 3330
MflI RGATCY 5 cut(s) 481, 1926, 2413, 3204, 3250
MhlI GDGCHC 3 cut(s) 1557, 2103, 2754
MlyI GAGTC 8 cut(s) 227, 421, 623, 1313, 1858, 2496, 2794, 3395
MmeI TCCRAC 2 cut(s) 396, 874
Mph1103I ATGCAT 2 cut(s) 1503, 1656
MroXI GAANNNNTTC 3 cut(s) 784, 1533, 2945
MslI CAYNNNNRTG 3 cut(s) 1655, 1712, 2747
MspA1I CMGCKG 1 cut(s) 3080
MspCI CTTAAG 1 cut(s) 1372
MspI CCGG 8 cut(s) 209, 280, 2095, 2105, 2849, 2985, 3305, 3341
MunI CAATTG 4 cut(s) 1431, 1473, 1830, 3330
Mva1269I GAATGC 3 cut(s) 981, 1440, 3072
MvaI CCWGG 7 cut(s) 996, 2283, 2636, 2773, 2795, 2835, 3209
MvnI CGCG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 485
NciI CCSGG 7 cut(s) 209, 210, 280, 2096, 2105, 2850, 3306
NdeI CATATG 2 cut(s) 948, 1656
NlaIV GGNNCC 6 cut(s) 287, 366, 883, 2597, 2847, 3206
NmuCI GTSAC 3 cut(s) 1514, 2420, 3004
NruI TCGCGA 1 cut(s) 238
NsiI ATGCAT 2 cut(s) 1503, 1656
NspI RCATGY 4 cut(s) 673, 691, 1118, 1654
PaeI GCATGC 2 cut(s) 673, 1118
PaeR7I CTCGAG 3 cut(s) 618, 1691, 2497
PciSI GCTCTTC 2 cut(s) 1206, 3423
PcsI WCGNNNNNNNCGW 2 cut(s) 235, 3371
PctI GAATGC 3 cut(s) 981, 1440, 3072
PdmI GAANNNNTTC 3 cut(s) 784, 1533, 2945
PfoI TCCNGGA 3 cut(s) 994, 2103, 2848
PkrI GCNGC 7 cut(s) 19, 761, 1872, 2767, 3470, 3501, 3514
PleI GAGTC 8 cut(s) 227, 421, 622, 1313, 1857, 2496, 2793, 3395
PmaCI CACGTG 1 cut(s) 3266
PmlI CACGTG 1 cut(s) 3266
PpsI GAGTC 8 cut(s) 227, 421, 622, 1313, 1857, 2496, 2793, 3395
Ppu21I YACGTR 1 cut(s) 3266
PpuMI RGGWCCY 1 cut(s) 324
PshBI ATTAAT 1 cut(s) 633
PsiI TTATAA 1 cut(s) 729
Psp1406I AACGTT 2 cut(s) 530, 870
Psp5II RGGWCCY 1 cut(s) 324
Psp6I CCWGG 7 cut(s) 994, 2281, 2634, 2771, 2793, 2833, 3207
PspCI CACGTG 1 cut(s) 3266
PspFI CCCAGC 1 cut(s) 488
PspGI CCWGG 7 cut(s) 994, 2281, 2634, 2771, 2793, 2833, 3207
PspN4I GGNNCC 6 cut(s) 287, 366, 883, 2597, 2847, 3206
PspPI GGNCC 3 cut(s) 148, 324, 2307
PspPPI RGGWCCY 1 cut(s) 324
PsrI GAACNNNNNNTAC 2 cut(s) 65, 97
PstI CTGCAG 1 cut(s) 1669
PstNI CAGNNNCTG 2 cut(s) 1303, 3492
PsuI RGATCY 5 cut(s) 481, 1926, 2413, 3204, 3250
PvuII CAGCTG 1 cut(s) 3080
RruI TCGCGA 1 cut(s) 238
RsaI GTAC 9 cut(s) 883, 1025, 1173, 1621, 1718, 1749, 2651, 3314, 3456
RsaNI GTAC 9 cut(s) 882, 1024, 1172, 1620, 1717, 1748, 2650, 3313, 3455
RseI CAYNNNNRTG 3 cut(s) 1655, 1712, 2747
SapI GCTCTTC 2 cut(s) 1206, 3423
SatI GCNGC 7 cut(s) 18, 760, 1871, 2766, 3469, 3500, 3513
Sau96I GGNCC 3 cut(s) 148, 324, 2307
SchI GAGTC 8 cut(s) 227, 421, 623, 1313, 1858, 2496, 2794, 3395
SduI GDGCHC 3 cut(s) 1557, 2103, 2754
SfaNI GCATC 6 cut(s) 952, 1381, 1488, 2147, 2339, 3291
SfcI CTRYAG 1 cut(s) 1665
Sfr274I CTCGAG 3 cut(s) 618, 1691, 2497
SgrAI CRCCGGYG 1 cut(s) 2984
SinI GGWCC 3 cut(s) 148, 324, 2307
SlaI CTCGAG 3 cut(s) 618, 1691, 2497
SmaI CCCGGG 1 cut(s) 210
SmiMI CAYNNNNRTG 3 cut(s) 1655, 1712, 2747
SmlI CTYRAG 7 cut(s) 85, 185, 618, 1372, 1691, 1882, 2497
SmoI CTYRAG 7 cut(s) 85, 185, 618, 1372, 1691, 1882, 2497
SphI GCATGC 2 cut(s) 673, 1118
SsiI CCGC 3 cut(s) 36, 434, 3499
SspMI CTAG 7 cut(s) 170, 414, 485, 1844, 2417, 2531, 3561
StyI CCWWGG 2 cut(s) 151, 3560
TaaI ACNGT 4 cut(s) 2856, 2899, 2972, 3244
TaiI ACGT 9 cut(s) 363, 378, 533, 873, 1177, 1567, 2550, 3268, 3314
TaqI TCGA 6 cut(s) 229, 619, 1692, 2361, 2498, 2932
TaqII GACCGA 1 cut(s) 642
TatI WGTACW 3 cut(s) 1716, 1747, 2649
TauI GCSGC 1 cut(s) 3502
TscAI CASTG 5 cut(s) 1669, 1762, 2187, 2595, 2622
TseFI GTSAC 3 cut(s) 1514, 2420, 3004
TseI GCWGC 6 cut(s) 17, 759, 1870, 2765, 3468, 3512
Tsp45I GTSAC 3 cut(s) 1514, 2420, 3004
TspGWI ACGGA 2 cut(s) 731, 3148
TspMI CCCGGG 1 cut(s) 208
TspRI CASTG 5 cut(s) 1669, 1762, 2187, 2595, 2622
Vha464I CTTAAG 1 cut(s) 1372
VneI GTGCAC 1 cut(s) 2750
VpaK11BI GGWCC 3 cut(s) 148, 324, 2307
VspI ATTAAT 1 cut(s) 633
XagI CCTNNNNNAGG 1 cut(s) 1037
XapI RAATTY 6 cut(s) 719, 783, 1772, 1808, 2232, 2928
XbaI TCTAGA 2 cut(s) 169, 1843
XceI RCATGY 4 cut(s) 673, 691, 1118, 1654
XcmI CCANNNNNNNNNTGG 3 cut(s) 602, 2632, 2722
XhoI CTCGAG 3 cut(s) 618, 1691, 2497
XmaI CCCGGG 1 cut(s) 208
XmaJI CCTAGG 1 cut(s) 3560
XmnI GAANNNNTTC 3 cut(s) 784, 1533, 2945
XspI CTAG 7 cut(s) 170, 414, 485, 1844, 2417, 2531, 3561
Zsp2I ATGCAT 2 cut(s) 1503, 1656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.