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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 276 |
| AccI | GTMKAC | 1 cut(s) | 357 |
| AciI | CCGC | 1 cut(s) | 100 |
| AclI | AACGTT | 1 cut(s) | 107 |
| AclWI | GGATC | 2 cut(s) | 211, 292 |
| AcsI | RAATTY | 1 cut(s) | 47 |
| AcuI | CTGAAG | 1 cut(s) | 67 |
| AcvI | CACGTG | 1 cut(s) | 370 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 372 |
| AflIII | ACRYGT | 1 cut(s) | 69 |
| AgsI | TTSAA | 2 cut(s) | 136, 305 |
| AjiI | CACGTC | 1 cut(s) | 70 |
| AluBI | AGCT | 4 cut(s) | 81, 223, 233, 506 |
| AluI | AGCT | 4 cut(s) | 81, 223, 233, 506 |
| AlwI | GGATC | 2 cut(s) | 211, 292 |
| AoxI | GGCC | 2 cut(s) | 197, 314 |
| ApoI | RAATTY | 1 cut(s) | 47 |
| AspS9I | GGNCC | 1 cut(s) | 325 |
| AsuC2I | CCSGG | 1 cut(s) | 201 |
| AsuHPI | GGTGA | 1 cut(s) | 451 |
| AvaII | GGWCC | 1 cut(s) | 325 |
| BanI | GGYRCC | 1 cut(s) | 276 |
| BauI | CACGAG | 2 cut(s) | 8, 228 |
| BbrPI | CACGTG | 1 cut(s) | 370 |
| BbsI | GAAGAC | 2 cut(s) | 99, 337 |
| BccI | CCATC | 3 cut(s) | 267, 441, 503 |
| BcnI | CCSGG | 1 cut(s) | 201 |
| BfaI | CTAG | 1 cut(s) | 479 |
| BfmI | CTRYAG | 1 cut(s) | 206 |
| BglII | AGATCT | 1 cut(s) | 475 |
| Bme1390I | CCNGG | 1 cut(s) | 201 |
| Bme18I | GGWCC | 1 cut(s) | 325 |
| BmgBI | CACGTC | 1 cut(s) | 70 |
| BmgT120I | GGNCC | 1 cut(s) | 325 |
| BmiI | GGNNCC | 2 cut(s) | 28, 278 |
| BmrFI | CCNGG | 1 cut(s) | 201 |
| BmsI | GCATC | 1 cut(s) | 272 |
| BpiI | GAAGAC | 2 cut(s) | 99, 337 |
| BpuEI | CTTGAG | 3 cut(s) | 6, 97, 197 |
| BpuMI | CCSGG | 1 cut(s) | 201 |
| BsaAI | YACGTR | 2 cut(s) | 331, 370 |
| BsaJI | CCNNGG | 1 cut(s) | 321 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 372 |
| Bse1I | ACTGG | 1 cut(s) | 341 |
| BseDI | CCNNGG | 1 cut(s) | 321 |
| BseGI | GGATG | 2 cut(s) | 266, 452 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 372 |
| BseNI | ACTGG | 1 cut(s) | 341 |
| BseRI | GAGGAG | 2 cut(s) | 22, 198 |
| BshFI | GGCC | 2 cut(s) | 199, 316 |
| BshNI | GGYRCC | 1 cut(s) | 276 |
| BsiSI | CCGG | 2 cut(s) | 200, 431 |
| BslFI | GGGAC | 2 cut(s) | 216, 349 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 372 |
| BsmFI | GGGAC | 2 cut(s) | 216, 349 |
| BsnI | GGCC | 2 cut(s) | 199, 316 |
| Bsp143I | GATC | 3 cut(s) | 216, 297, 475 |
| BspACI | CCGC | 1 cut(s) | 100 |
| BspANI | GGCC | 2 cut(s) | 199, 316 |
| BspLI | GGNNCC | 2 cut(s) | 28, 278 |
| BspMAI | CTGCAG | 1 cut(s) | 210 |
| BspPI | GGATC | 2 cut(s) | 211, 292 |
| BspQI | GCTCTTC | 1 cut(s) | 391 |
| BspT107I | GGYRCC | 1 cut(s) | 276 |
| BsrI | ACTGG | 1 cut(s) | 341 |
| BssECI | CCNNGG | 1 cut(s) | 321 |
| BssMI | GATC | 3 cut(s) | 216, 297, 475 |
| BssSI | CACGAG | 2 cut(s) | 8, 228 |
| Bst2BI | CACGAG | 2 cut(s) | 8, 228 |
| Bst6I | CTCTTC | 1 cut(s) | 391 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 470 |
| BstBAI | YACGTR | 2 cut(s) | 331, 370 |
| BstC8I | GCNNGC | 1 cut(s) | 484 |
| BstF5I | GGATG | 2 cut(s) | 266, 452 |
| BstKTI | GATC | 3 cut(s) | 219, 300, 478 |
| BstMBI | GATC | 3 cut(s) | 216, 297, 475 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 87, 205, 470, 479 |
| BstSCI | CCNGG | 1 cut(s) | 199 |
| BstSFI | CTRYAG | 1 cut(s) | 206 |
| BstV2I | GAAGAC | 2 cut(s) | 99, 337 |
| BstX2I | RGATCY | 1 cut(s) | 475 |
| BstYI | RGATCY | 1 cut(s) | 475 |
| BsuRI | GGCC | 2 cut(s) | 199, 316 |
| BtrI | CACGTC | 1 cut(s) | 70 |
| BtsCI | GGATG | 2 cut(s) | 266, 452 |
| Cac8I | GCNNGC | 1 cut(s) | 484 |
| Cfr13I | GGNCC | 1 cut(s) | 325 |
| CviAII | CATG | 2 cut(s) | 353, 487 |
| CviJI | RGCY | 8 cut(s) | 5, 81, 131, 199, 223, 233, 316, 506 |
| CviKI_1 | RGCY | 8 cut(s) | 5, 81, 131, 199, 223, 233, 316, 506 |
| DpnI | GATC | 3 cut(s) | 218, 299, 477 |
| DpnII | GATC | 3 cut(s) | 216, 297, 475 |
| Eam1104I | CTCTTC | 1 cut(s) | 391 |
| EarI | CTCTTC | 1 cut(s) | 391 |
| Eco147I | AGGCCT | 1 cut(s) | 316 |
| Eco47I | GGWCC | 1 cut(s) | 325 |
| Eco57I | CTGAAG | 1 cut(s) | 67 |
| Eco72I | CACGTG | 1 cut(s) | 370 |
| FaeI | CATG | 2 cut(s) | 356, 490 |
| FaiI | YATR | 4 cut(s) | 192, 194, 354, 488 |
| FaqI | GGGAC | 2 cut(s) | 216, 349 |
| FatI | CATG | 2 cut(s) | 352, 486 |
| FauI | CCCGC | 1 cut(s) | 107 |
| FblI | GTMKAC | 1 cut(s) | 357 |
| FokI | GGATG | 2 cut(s) | 253, 459 |
| FspBI | CTAG | 1 cut(s) | 479 |
| HaeIII | GGCC | 2 cut(s) | 199, 316 |
| HapII | CCGG | 2 cut(s) | 200, 431 |
| Hin1II | CATG | 2 cut(s) | 356, 490 |
| HincII | GTYRAC | 1 cut(s) | 358 |
| HindII | GTYRAC | 1 cut(s) | 358 |
| HinfI | GANTC | 1 cut(s) | 38 |
| HpaII | CCGG | 2 cut(s) | 200, 431 |
| HphI | GGTGA | 1 cut(s) | 451 |
| Hpy166II | GTNNAC | 2 cut(s) | 111, 358 |
| Hpy188I | TCNGA | 4 cut(s) | 37, 86, 253, 325 |
| Hpy188III | TCNNGA | 4 cut(s) | 10, 76, 228, 270 |
| Hpy8I | GTNNAC | 2 cut(s) | 111, 358 |
| Hpy99I | CGWCG | 1 cut(s) | 71 |
| HpyAV | CCTTC | 2 cut(s) | 436, 448 |
| HpyCH4IV | ACGT | 5 cut(s) | 69, 107, 155, 330, 369 |
| HpyCH4V | TGCA | 4 cut(s) | 208, 407, 464, 473 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 87, 205, 470, 479 |
| HpySE526I | ACGT | 5 cut(s) | 69, 107, 155, 330, 369 |
| Hsp92II | CATG | 2 cut(s) | 356, 490 |
| Kzo9I | GATC | 3 cut(s) | 216, 297, 475 |
| LguI | GCTCTTC | 1 cut(s) | 391 |
| LpnPI | CCDG | 8 cut(s) | 129, 213, 233, 283, 354, 428, 444, 468 |
| LweI | GCATC | 1 cut(s) | 272 |
| MaeI | CTAG | 1 cut(s) | 479 |
| MaeII | ACGT | 5 cut(s) | 69, 107, 155, 330, 369 |
| MaeIII | GTNAC | 3 cut(s) | 10, 161, 225 |
| MalI | GATC | 3 cut(s) | 218, 299, 477 |
| MboI | GATC | 3 cut(s) | 216, 297, 475 |
| MboII | GAAGA | 4 cut(s) | 104, 266, 337, 408 |
| MflI | RGATCY | 1 cut(s) | 475 |
| MluCI | AATT | 1 cut(s) | 47 |
| MslI | CAYNNNNRTG | 1 cut(s) | 271 |
| MspA1I | CMGCKG | 1 cut(s) | 223 |
| MspI | CCGG | 2 cut(s) | 200, 431 |
| MspR9I | CCNGG | 1 cut(s) | 201 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 87, 205, 470, 479 |
| NciI | CCSGG | 1 cut(s) | 201 |
| NdeII | GATC | 3 cut(s) | 216, 297, 475 |
| NlaIII | CATG | 2 cut(s) | 356, 490 |
| NlaIV | GGNNCC | 2 cut(s) | 28, 278 |
| NmuCI | GTSAC | 3 cut(s) | 10, 161, 225 |
| PceI | AGGCCT | 1 cut(s) | 316 |
| PciSI | GCTCTTC | 1 cut(s) | 391 |
| PfeI | GAWTC | 1 cut(s) | 38 |
| PmaCI | CACGTG | 1 cut(s) | 370 |
| PmlI | CACGTG | 1 cut(s) | 370 |
| Ppu21I | YACGTR | 2 cut(s) | 331, 370 |
| Psp1406I | AACGTT | 1 cut(s) | 107 |
| PspCI | CACGTG | 1 cut(s) | 370 |
| PspN4I | GGNNCC | 2 cut(s) | 28, 278 |
| PspPI | GGNCC | 1 cut(s) | 325 |
| PstI | CTGCAG | 1 cut(s) | 210 |
| PsuI | RGATCY | 1 cut(s) | 475 |
| PvuII | CAGCTG | 1 cut(s) | 223 |
| RseI | CAYNNNNRTG | 1 cut(s) | 271 |
| SalI | GTCGAC | 1 cut(s) | 356 |
| SapI | GCTCTTC | 1 cut(s) | 391 |
| Sau3AI | GATC | 3 cut(s) | 216, 297, 475 |
| Sau96I | GGNCC | 1 cut(s) | 325 |
| ScrFI | CCNGG | 1 cut(s) | 201 |
| SfaNI | GCATC | 1 cut(s) | 272 |
| SfcI | CTRYAG | 1 cut(s) | 206 |
| SinI | GGWCC | 1 cut(s) | 325 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 271 |
| SmlI | CTYRAG | 3 cut(s) | 21, 76, 176 |
| SmoI | CTYRAG | 3 cut(s) | 21, 76, 176 |
| Sse9I | AATT | 1 cut(s) | 47 |
| SseBI | AGGCCT | 1 cut(s) | 316 |
| SsiI | CCGC | 1 cut(s) | 100 |
| SspMI | CTAG | 1 cut(s) | 479 |
| StuI | AGGCCT | 1 cut(s) | 316 |
| StyD4I | CCNGG | 1 cut(s) | 199 |
| TaiI | ACGT | 5 cut(s) | 72, 110, 158, 333, 372 |
| TaqI | TCGA | 1 cut(s) | 357 |
| TasI | AATT | 1 cut(s) | 47 |
| TfiI | GAWTC | 1 cut(s) | 38 |
| TseFI | GTSAC | 3 cut(s) | 10, 161, 225 |
| Tsp45I | GTSAC | 3 cut(s) | 10, 161, 225 |
| TspDTI | ATGAA | 2 cut(s) | 186, 300 |
| TspGWI | ACGGA | 1 cut(s) | 149 |
| VpaK11BI | GGWCC | 1 cut(s) | 325 |
| XapI | RAATTY | 1 cut(s) | 47 |
| XmiI | GTMKAC | 1 cut(s) | 357 |
| XspI | CTAG | 1 cut(s) | 479 |