MD07G1112300.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000036
Physical Location & Seq
Reverse (-)
13044214 .. 13048883
4670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1112300.v1.1.491

Sequence Viewer

Length: 3519 bp
ATGGCTGTCGTTAGAGATCGAGAAGCTTCGTCTTCCGATTCTAGTACTTATCACGTGTTCTTGAGTTTTAGAGGCAAAGACACTCGCAAGACTTTTACTGACCACCTCTATGAAGCCTTTGTCAGTGCAGGCCTTCGAACTTTCCGAGATGATGATGAACTTGAGAGAGGGGAAGATATTAAGTCGGAGCTGCCGATAGCAATCCAACACTCTCGAAGTTCAGTCATAGTGTTTTCGAAAGACTACGCTTCTTCCAAGTGGTGCCTTGACGAGCTTGTGATGATCCTTGAACGGAAAAAGGCCTCTAAGCATGTAGTTATACCAGTGTTTTATGACATTGATCCATCGGAGGTGAGGAAGCAGACTGGAACCTTTGCGAAAGCATTTTCTAGACACCAAAAAAATCTATCTTTGAGCAAGGACAGGGTAAATGGATGGAGGGCAGCACTTGCAGAAGTTGCAGATCTAGCAGGGATGGTCTTACAAAATGAAAATGACGGGCACGAGGCGCAGTTTATCAAGAAAATTGTTAAAGTGATTGAAGCCAAATTAAGTCGCACACCATTAAGTGTTGCCCCTTACCTGGTTGGTATCAATTCTCGAGTAGAAGAGATTACTTTATGGTTACAAGATGGATCATCCGATGTTGGCATATTTGTAATTTGTGGTATTGGTGGAATAGGGAAGACAACCATTGCGCAAGTTGTTTACAATTCAAATTTTTCAAGATTTGAAGGAAGAAGCTTCCTTGAAAATATCAGGGAAATTTCAGAAAGACCTAATGGCTTGGTACAAGTTCAAATGCAACTTCTCTATGATATATTGGGTGGGAGAGAAGTGAAGATACATAGTATAAGCGAGGGAATAATTAAGATTAAAGATGTCATTAGCTGTAAAAAAGTTTTTCTCGTTCTTGATGATGTAGATCATACGAGCCAATTAGATGCAATACTTAGAATGCGAGATTGGTTTTATCCAGGTAGTAAAATCATTATAACAACTAGGCGTGCAGGGTTGTTAAAGTCTCAGAAGGCTGTTAAGGTACATAATGTTAAAACTCTAAATCATGTTGAATCATTGGAGCTCTTTAGTTGGCATGCTTTTGGACAAGACCATCCAATTGATGGTTACATCGAGCTTTCAGAAAGAGTGGTAAGCAATAGCGGAGGGCTTCCATTAGCTCTTCAAGTTTTGGGTTCCTCTCTATCAGGGCAAAATTTAGATGTATGGGAAAGTGCATTGAAGAAATTGGAAGCTATTCCAAATAGTGACATCGTGAATAAGCTTAGAATAAGCTACGATTCTTTACAAGATGACCATGACAAAAATTTATTCCTCCACATTGCATGTTTCTTTATTGGAAAGGATAAGGATGTCATTGTCAAAATACTAGATGGATGCGGCTTCTTTACAACTGTTGGCATTCAAAATCTCAGTGACCGATGTTTGGTAACAGTTGATGAATATAATAAAGTGAAGATGCATCAAATGATTCGGGACATGGGAAGAGAAATTGTTCGCCAAGAATCGAAAGAGCTTGAGAAACGTAGCAGATTATGGAATCATAACGATTCTCTCAATGTCTTGAGAGAAAAGAATGGTTCAAAAAAAATTGAAGGTCTTGCCCTGAACATGCATCTTGTAGACCACCCTTCAAGAAAATCGAATATGGTTGTCTTGGAAACCAAGGCATTTAAAAGGATGGTCAAGTTGAGATTGCTCCAGCTTAGTTATGTACAACTTAATGGATGCTATGAAGAATTTCCTAATGGATTGAGATGGTTGTATTGGCTTAAATTCCCCTTGGACTCTATACCTAGTGATCTTCTTTTAGAGAGCTTGGTTGTTCTTGAAATGCACTACAGCAGCTTAAGACAAATATGGAAGGGAACAAAGTATCTTCCAGCATTAAAGATCCTTGATCTCATCCATTCCCATGATCTCATTGAAACCCCTGACTTCTCATCGGTCCCCAATCTAGAAAGATTTAGTCTTGAAGATTGTGTAAGTTTGGTTTATGTCCATGAATCCATTGGAAATCTAGAGAAGCTTGTTTACTTGAATATGAAAGATTGCAGAAATATTACGAAGCTTCCGAAGAGCATTTCTATGCTTAAGTCACTGGAAACGCTTATTGTATCTGGTTGTTTAAGTCTTATTGATTTTTCCATGGAAATAGGAAAGATGGAATCTCTAAAAGTGTTTCTAGGAGATCAAATTCCAATTATTAGATTACACACTACCACTTTACCCTGCACCTTAGTGGTTTTAAGTTTAACGGAATGCAATCTTTCTGATGATGCTTTTCCAAGGGATTTTGGTAACTTACCCTCCTTGCAGAGATTGGATCTAAGTAGCAATCCAATTTGTAACCTACCAGATTGCATCAGAGGCCTTACGGGGCTCGATCACCTTGCATTTTCCCAGTGTTCGAAGCTCAAATCGCTTGTGGGACTACCGAGAGTTGCAGAATTAGTTACTGTCCATTCTGAATCCTTGGAAAAAATAACATTTCAAACAATTTCGTGCATCCCGGAGAGGTTTATGCATGGTTACAACTTTAAACTTGCTGAGGTTGAGTACTGGTATAAGTTAGAACCCATTGAAAGAGTTGATGTAGAAATGATCAAACTTTTGGGATTGTGCAACTTGGAATCCATGAAAGCCATCAGGATGTTCATCCCAGATATGCTATATCGGAATGGCATGATCCGTCCCATTGAGGGATTGTATGAATATGGTATATTCAGCACATTTCTTCCTGGAAACGAGGTTCCAGGCCGGTTCAGTCATACAAGTAAAGGGTCCTCAATATCTTTTACTTTGCCTTCACTTCCTAATCTCAAGATCCAAGGCTTGAATATCTTCTCCGTCTATGCAAATTCTGGTGTGTATCATTTTTCTAGTATGCCTAATCCAGTAATGATCAAAATAAGTAACCAAAGCAAGGACCTTAAGTGGATCTATGGCCCCTCCTGCTACGGCATTCCAGATGATGGAAACGACATGACATGGTTAAGCCACTGGAAGTTGGGGAATCGATTAGAATGTGGCGATGAAGTGACTGTTTCCGTATTTACACGTCCTCCATTTCTAGTGAAAGAGTGCGGCATCCAACTTGTGCACGAGCGAGAAGTCGAGATCATGAGCACCCAAGACTACAACATAGACCCTTGCCATTCCTTTTATAAGGATTTGGAAGAATTTGTTCCAGGAACGTACTTCCTGTGGGGTGGACCCATGGTGAAAGATAAAGATTATGTTTTTAGTTGGCAAAAGGAAGATTGGTTCCGCGGCATCTTTGGAGACTCTAATCAAGAGAATGACCAAGAAGAAGGGCAGCAGGTGGGCGATAAGCCGCTTGCGATTGCAGTTGCAGCAGCAGAGGCATTGGCAGAAGCAGAGAGAGCAAGCAATAGCGGCGGTCGAAGAGGCTGCAAGATTTTCGTTATATTCACCGCTGTCTTCTTTCTCTTCGTTTCTTTAGTATTCTCTCGCTTTCCTCTCTCTCAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1173

Amino Acids

132.88

Weight (kDa)

6.26

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 17 - 190 3.8e-50 TIR domain
TIR_2 PF13676 19 - 115 6e-16 TIR domain
NB-ARC PF00931 200 - 369 9.3e-17 NB-ARC domain
WHD_ROQ1 PF23282 439 - 510 1.4e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 634 - 837 6.2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 648 - 738 3.8e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 995, 3189
AarI CACCTGC 1 cut(s) 3334
Acc16I TGCGCA 1 cut(s) 699
Acc36I ACCTGC 1 cut(s) 3334
AccB1I GGYRCC 1 cut(s) 261
AccB7I CCANNNNNTGG 2 cut(s) 1124, 2996
AccI GTMKAC 1 cut(s) 1644
AccII CGCG 1 cut(s) 3294
AciI CCGC 9 cut(s) 1164, 1401, 3108, 3292, 3294, 3359, 3420, 3423, 3459
AclWI GGATC 8 cut(s) 277, 335, 643, 1909, 2355, 2704, 2842, 2969
AcsI RAATTY 9 cut(s) 718, 765, 1216, 1327, 1760, 1796, 2217, 2881, 3203
AcvI CACGTG 1 cut(s) 55
AfaI GTAC 6 cut(s) 46, 792, 1044, 1737, 2582, 3221
AfiI CCNNNNNNNGG 6 cut(s) 583, 1124, 1447, 2723, 2947, 2996
AflII CTTAAG 3 cut(s) 1870, 2114, 2954
AflIII ACRYGT 2 cut(s) 54, 3080
AjiI CACGTC 1 cut(s) 3083
AjnI CCWGG 5 cut(s) 582, 976, 2761, 2776, 3211
AjuI GAANNNNNNNTTGG 2 cut(s) 949, 981
AleI CACNNNNGTG 1 cut(s) 2261
AloI GAACNNNNNNTCC 4 cut(s) 2757, 2789, 3272, 3304
Alw21I GWGCWC 3 cut(s) 1086, 3126, 3152
Alw26I GTCTC 2 cut(s) 1029, 3300
Alw44I GTGCAC 1 cut(s) 3122
AlwI GGATC 8 cut(s) 277, 335, 643, 1909, 2355, 2704, 2842, 2969
Ama87I CYCGRG 1 cut(s) 600
AoxI GGCC 5 cut(s) 130, 300, 2392, 2779, 2968
ApaLI GTGCAC 1 cut(s) 3122
ApeKI GCWGC 7 cut(s) 190, 443, 1866, 3340, 3377, 3380, 3435
ApoI RAATTY 9 cut(s) 718, 765, 1216, 1327, 1760, 1796, 2217, 2881, 3203
Asp700I GAANNNNTTC 5 cut(s) 1257, 1515, 1761, 2864, 3207
AspLEI GCGC 2 cut(s) 511, 700
AspS9I GGNCC 5 cut(s) 1969, 2805, 2950, 2969, 3236
AsuC2I CCSGG 1 cut(s) 2534
AsuHPI GGTGA 4 cut(s) 364, 2402, 3256, 3448
AsuII TTCGAA 3 cut(s) 136, 236, 2432
AvaI CYCGRG 1 cut(s) 600
AvaII GGWCC 4 cut(s) 1969, 2805, 2950, 3236
BaeGI GKGCMC 2 cut(s) 504, 3126
BanI GGYRCC 1 cut(s) 261
BanII GRGCYC 2 cut(s) 1086, 2406
BarI GAAGNNNNNNTAC 4 cut(s) 828, 860, 2039, 2071
BauI CACGAG 2 cut(s) 503, 3125
BbrPI CACGTG 1 cut(s) 55
BbsI GAAGAC 3 cut(s) 24, 692, 3457
Bbv12I GWGCWC 3 cut(s) 1086, 3126, 3152
BbvCI CCTCAGC 1 cut(s) 2571
BbvI GCAGC 7 cut(s) 177, 455, 1878, 3352, 3389, 3392, 3422
BceAI ACGGC 1 cut(s) 2998
BcgI CGANNNNNNTGC 6 cut(s) 2396, 2430, 3417, 3427, 3451, 3461
BciT130I CCWGG 5 cut(s) 584, 978, 2763, 2778, 3213
BclI TGATCA 2 cut(s) 2625, 2925
BcnI CCSGG 1 cut(s) 2534
BcoDI GTCTC 2 cut(s) 1029, 3300
BfmI CTRYAG 1 cut(s) 1861
BfrI CTTAAG 3 cut(s) 1870, 2114, 2954
BfuAI ACCTGC 1 cut(s) 3334
BglII AGATCT 1 cut(s) 463
BmcAI AGTACT 2 cut(s) 46, 2582
Bme1390I CCNGG 6 cut(s) 584, 978, 2534, 2763, 2778, 3213
Bme18I GGWCC 4 cut(s) 1969, 2805, 2950, 3236
BmeT110I CYCGRG 1 cut(s) 600
BmgBI CACGTC 1 cut(s) 3083
BmgT120I GGNCC 5 cut(s) 1969, 2805, 2950, 2969, 3236
BmiI GGNNCC 9 cut(s) 263, 370, 1198, 1971, 2775, 2806, 2971, 3238, 3290
BmrFI CCNGG 6 cut(s) 584, 978, 2534, 2763, 2778, 3213
BmrI ACTGGG 1 cut(s) 2419
BmuI ACTGGG 1 cut(s) 2419
BpiI GAAGAC 3 cut(s) 24, 692, 3457
BpmI CTGGAG 1 cut(s) 1706
Bpu10I CCTNAGC 1 cut(s) 2571
Bpu14I TTCGAA 3 cut(s) 136, 236, 2432
BpuEI CTTGAG 5 cut(s) 82, 182, 1559, 1606, 2828
BpuMI CCSGG 1 cut(s) 2534
Bsa29I ATCGAT 1 cut(s) 3040
BsaAI YACGTR 1 cut(s) 55
BsaBI GATNNNNATC 3 cut(s) 200, 924, 2678
BsaJI CCNNGG 8 cut(s) 1686, 1803, 2169, 2309, 2496, 2851, 3240, 3292
BsaXI ACNNNNNCTCC 4 cut(s) 2315, 2345, 3070, 3100
Bsc4I CCNNNNNNNGG 6 cut(s) 583, 1124, 1447, 2723, 2947, 2996
Bse118I RCCGGY 1 cut(s) 2781
Bse1I ACTGG 7 cut(s) 323, 370, 2127, 2425, 2588, 2918, 3029
Bse3DI GCAATG 2 cut(s) 693, 1341
Bse8I GATNNNNATC 3 cut(s) 200, 924, 2678
BseBI CCWGG 5 cut(s) 584, 978, 2763, 2778, 3213
BseCI ATCGAT 1 cut(s) 3040
BseDI CCNNGG 8 cut(s) 1686, 1803, 2169, 2309, 2496, 2851, 3240, 3292
BseJI GATNNNNATC 3 cut(s) 200, 924, 2678
BseLI CCNNNNNNNGG 6 cut(s) 583, 1124, 1447, 2723, 2947, 2996
BseMI GCAATG 2 cut(s) 693, 1341
BseMII CTCAG 3 cut(s) 1040, 1447, 2562
BseNI ACTGG 7 cut(s) 323, 370, 2127, 2425, 2588, 2918, 3029
BseSI GKGCMC 2 cut(s) 504, 3126
BseXI GCAGC 7 cut(s) 177, 455, 1878, 3352, 3389, 3392, 3422
BsgI GTGCAG 3 cut(s) 147, 1029, 2239
Bsh1236I CGCG 1 cut(s) 3294
Bsh1285I CGRYCG 1 cut(s) 3427
BshFI GGCC 5 cut(s) 132, 302, 2394, 2781, 2970
BshNI GGYRCC 1 cut(s) 261
BshVI ATCGAT 1 cut(s) 3040
BsiEI CGRYCG 1 cut(s) 3427
BsiHKAI GWGCWC 3 cut(s) 1086, 3126, 3152
BsiHKCI CYCGRG 1 cut(s) 600
BsiSI CCGG 2 cut(s) 2534, 2782
BslFI GGGAC 4 cut(s) 1511, 1955, 2466, 2700
BslI CCNNNNNNNGG 6 cut(s) 583, 1124, 1447, 2723, 2947, 2996
BsmAI GTCTC 2 cut(s) 1029, 3300
BsmFI GGGAC 4 cut(s) 1511, 1955, 2466, 2700
BsmI GAATGC 4 cut(s) 963, 1422, 2288, 2985
BsnI GGCC 5 cut(s) 132, 302, 2394, 2781, 2970
BsoBI CYCGRG 1 cut(s) 600
Bsp119I TTCGAA 3 cut(s) 136, 236, 2432
Bsp1286I GDGCHC 5 cut(s) 504, 1086, 2406, 3126, 3152
Bsp1407I TGTACA 1 cut(s) 1735
Bsp19I CCATGG 2 cut(s) 2169, 3240
BspACI CCGC 9 cut(s) 1164, 1401, 3108, 3292, 3294, 3359, 3420, 3423, 3459
BspANI GGCC 5 cut(s) 132, 302, 2394, 2781, 2970
BspCNI CTCAG 3 cut(s) 1039, 1446, 2563
BspDI ATCGAT 1 cut(s) 3040
BspFNI CGCG 1 cut(s) 3294
BspHI TCATGA 2 cut(s) 3144, 3515
BspLI GGNNCC 9 cut(s) 263, 370, 1198, 1971, 2775, 2806, 2971, 3238, 3290
BspMI ACCTGC 1 cut(s) 3334
BspPI GGATC 8 cut(s) 277, 335, 643, 1909, 2355, 2704, 2842, 2969
BspQI GCTCTTC 2 cut(s) 1188, 2093
BspT104I TTCGAA 3 cut(s) 136, 236, 2432
BspT107I GGYRCC 1 cut(s) 261
BspTI CTTAAG 3 cut(s) 1870, 2114, 2954
BsrDI GCAATG 2 cut(s) 693, 1341
BsrFI RCCGGY 1 cut(s) 2781
BsrGI TGTACA 1 cut(s) 1735
BsrI ACTGG 7 cut(s) 323, 370, 2127, 2425, 2588, 2918, 3029
BssAI RCCGGY 1 cut(s) 2781
BssECI CCNNGG 8 cut(s) 1686, 1803, 2169, 2309, 2496, 2851, 3240, 3292
BssSI CACGAG 2 cut(s) 503, 3125
BssT1I CCWWGG 7 cut(s) 1686, 1803, 2169, 2309, 2496, 2851, 3240
Bst2BI CACGAG 2 cut(s) 503, 3125
Bst2UI CCWGG 5 cut(s) 584, 978, 2763, 2778, 3213
Bst4CI ACNGT 4 cut(s) 1417, 1456, 2482, 3067
Bst6I CTCTTC 6 cut(s) 603, 1188, 1501, 2093, 3424, 3479
BstAFI CTTAAG 3 cut(s) 1870, 2114, 2954
BstAPI GCANNNNNTGC 2 cut(s) 449, 458
BstAUI TGTACA 1 cut(s) 1735
BstBAI YACGTR 1 cut(s) 55
BstBI TTCGAA 3 cut(s) 136, 236, 2432
BstC8I GCNNGC 5 cut(s) 130, 1008, 1098, 3363, 3412
BstDSI CCRYGG 3 cut(s) 2169, 3240, 3292
BstFNI CGCG 1 cut(s) 3294
BstHHI GCGC 2 cut(s) 511, 700
BstMAI GTCTC 2 cut(s) 1029, 3300
BstMCI CGRYCG 1 cut(s) 3427
BstNI CCWGG 5 cut(s) 584, 978, 2763, 2778, 3213
BstNSI RCATGY 4 cut(s) 314, 1100, 1350, 1636
BstSCI CCNGG 6 cut(s) 582, 976, 2532, 2761, 2776, 3211
BstSFI CTRYAG 1 cut(s) 1861
BstSLI GKGCMC 2 cut(s) 504, 3126
BstUI CGCG 1 cut(s) 3294
BstV1I GCAGC 7 cut(s) 177, 455, 1878, 3352, 3389, 3392, 3422
BstV2I GAAGAC 3 cut(s) 24, 692, 3457
BstX2I RGATCY 5 cut(s) 463, 1914, 2347, 2847, 2961
BstYI RGATCY 5 cut(s) 463, 1914, 2347, 2847, 2961
Bsu15I ATCGAT 1 cut(s) 3040
BsuRI GGCC 5 cut(s) 132, 302, 2394, 2781, 2970
BsuTUI ATCGAT 1 cut(s) 3040
BtgI CCRYGG 3 cut(s) 2169, 3240, 3292
BtgZI GCGATG 1 cut(s) 3069
BtrI CACGTC 1 cut(s) 3083
BtsIMutI CAGTG 6 cut(s) 130, 330, 1441, 2120, 2432, 3022
BveI ACCTGC 1 cut(s) 3334
Cac8I GCNNGC 5 cut(s) 130, 1008, 1098, 3363, 3412
CciI TCATGA 2 cut(s) 3144, 3515
CfoI GCGC 2 cut(s) 511, 700
Cfr10I RCCGGY 1 cut(s) 2781
Cfr13I GGNCC 5 cut(s) 1969, 2805, 2950, 2969, 3236
Cfr42I CCGCGG 1 cut(s) 3295
ClaI ATCGAT 1 cut(s) 3040
CsiI ACCWGGT 1 cut(s) 582
Csp6I GTAC 6 cut(s) 45, 791, 1043, 1736, 2581, 3220
CviQI GTAC 6 cut(s) 45, 791, 1043, 1736, 2581, 3220
DraI TTTAAA 2 cut(s) 1696, 2563
Eam1104I CTCTTC 6 cut(s) 603, 1188, 1501, 2093, 3424, 3479
EarI CTCTTC 6 cut(s) 603, 1188, 1501, 2093, 3424, 3479
Ecl136II GAGCTC 1 cut(s) 1084
Eco130I CCWWGG 7 cut(s) 1686, 1803, 2169, 2309, 2496, 2851, 3240
Eco147I AGGCCT 3 cut(s) 132, 302, 2394
Eco24I GRGCYC 2 cut(s) 1086, 2406
Eco47I GGWCC 4 cut(s) 1969, 2805, 2950, 3236
Eco53kI GAGCTC 1 cut(s) 1084
Eco72I CACGTG 1 cut(s) 55
Eco88I CYCGRG 1 cut(s) 600
EcoICRI GAGCTC 1 cut(s) 1084
EcoO109I RGGNCCY 2 cut(s) 2805, 2950
EcoRII CCWGG 5 cut(s) 582, 976, 2761, 2776, 3211
EcoT14I CCWWGG 7 cut(s) 1686, 1803, 2169, 2309, 2496, 2851, 3240
EcoT22I ATGCAT 3 cut(s) 1485, 1638, 2550
EcoT38I GRGCYC 2 cut(s) 1086, 2406
ErhI CCWWGG 7 cut(s) 1686, 1803, 2169, 2309, 2496, 2851, 3240
FaqI GGGAC 4 cut(s) 1511, 1955, 2466, 2700
FbaI TGATCA 2 cut(s) 2625, 2925
FblI GTMKAC 1 cut(s) 1644
FriOI GRGCYC 2 cut(s) 1086, 2406
FspI TGCGCA 1 cut(s) 699
GlaI GCGC 2 cut(s) 510, 699
GsuI CTGGAG 1 cut(s) 1706
HaeIII GGCC 5 cut(s) 132, 302, 2394, 2781, 2970
HapII CCGG 2 cut(s) 2534, 2782
HhaI GCGC 2 cut(s) 511, 700
Hin6I GCGC 2 cut(s) 509, 698
HinP1I GCGC 2 cut(s) 509, 698
HindIII AAGCTT 5 cut(s) 24, 742, 1283, 2048, 2090
HpaII CCGG 2 cut(s) 2534, 2782
HphI GGTGA 4 cut(s) 364, 2402, 3256, 3448
Hpy166II GTNNAC 5 cut(s) 709, 1645, 2056, 3124, 3236
Hpy8I GTNNAC 5 cut(s) 709, 1645, 2056, 3124, 3236
HpyAV CCTTC 8 cut(s) 143, 728, 1024, 1610, 1662, 1879, 2838, 3329
HpyCH4III ACNGT 4 cut(s) 1417, 1456, 2482, 3067
HpyCH4IV ACGT 4 cut(s) 54, 1546, 3082, 3218
HpySE526I ACGT 4 cut(s) 54, 1546, 3082, 3218
HspAI GCGC 2 cut(s) 509, 698
Ksp22I TGATCA 2 cut(s) 2625, 2925
KspI CCGCGG 1 cut(s) 3295
LguI GCTCTTC 2 cut(s) 1188, 2093
LmnI GCTCC 3 cut(s) 187, 1081, 1725
Lsp1109I GCAGC 7 cut(s) 177, 455, 1878, 3352, 3389, 3392, 3422
MabI ACCWGGT 1 cut(s) 582
MaeII ACGT 4 cut(s) 54, 1546, 3082, 3218
MfeI CAATTG 1 cut(s) 1119
MflI RGATCY 5 cut(s) 463, 1914, 2347, 2847, 2961
MhlI GDGCHC 5 cut(s) 504, 1086, 2406, 3126, 3152
MlyI GAGTC 2 cut(s) 1802, 3302
MmeI TCCRAC 3 cut(s) 165, 229, 3139
Mph1103I ATGCAT 3 cut(s) 1485, 1638, 2550
MroXI GAANNNNTTC 5 cut(s) 1257, 1515, 1761, 2864, 3207
MslI CAYNNNNRTG 5 cut(s) 108, 1935, 2108, 2261, 2672
MspA1I CMGCKG 2 cut(s) 3294, 3461
MspCI CTTAAG 3 cut(s) 1870, 2114, 2954
MspI CCGG 2 cut(s) 2534, 2782
MspR9I CCNGG 6 cut(s) 584, 978, 2534, 2763, 2778, 3213
MunI CAATTG 1 cut(s) 1119
Mva1269I GAATGC 4 cut(s) 963, 1422, 2288, 2985
MvaI CCWGG 5 cut(s) 584, 978, 2763, 2778, 3213
MvnI CGCG 1 cut(s) 3294
NciI CCSGG 1 cut(s) 2534
NcoI CCATGG 2 cut(s) 2169, 3240
NlaIV GGNNCC 9 cut(s) 263, 370, 1198, 1971, 2775, 2806, 2971, 3238, 3290
NmuCI GTSAC 4 cut(s) 1268, 1436, 2118, 3061
NsbI TGCGCA 1 cut(s) 699
NsiI ATGCAT 3 cut(s) 1485, 1638, 2550
NspI RCATGY 4 cut(s) 314, 1100, 1350, 1636
NspV TTCGAA 3 cut(s) 136, 236, 2432
OliI CACNNNNGTG 1 cut(s) 2261
PaeI GCATGC 1 cut(s) 1100
PaeR7I CTCGAG 1 cut(s) 600
PagI TCATGA 2 cut(s) 3144, 3515
PaqCI CACCTGC 1 cut(s) 3334
PceI AGGCCT 3 cut(s) 132, 302, 2394
PciSI GCTCTTC 2 cut(s) 1188, 2093
PcsI WCGNNNNNNNCGW 3 cut(s) 142, 191, 2093
PctI GAATGC 4 cut(s) 963, 1422, 2288, 2985
PdmI GAANNNNTTC 5 cut(s) 1257, 1515, 1761, 2864, 3207
PflMI CCANNNNNTGG 2 cut(s) 1124, 2996
PfoI TCCNGGA 3 cut(s) 2532, 2761, 3211
PleI GAGTC 2 cut(s) 1802, 3302
PmaCI CACGTG 1 cut(s) 55
PmlI CACGTG 1 cut(s) 55
PpsI GAGTC 2 cut(s) 1802, 3302
Ppu21I YACGTR 1 cut(s) 55
PpuMI RGGWCCY 2 cut(s) 2805, 2950
PsiI TTATAA 2 cut(s) 995, 3189
Psp124BI GAGCTC 1 cut(s) 1086
Psp5II RGGWCCY 2 cut(s) 2805, 2950
Psp6I CCWGG 5 cut(s) 582, 976, 2761, 2776, 3211
PspCI CACGTG 1 cut(s) 55
PspGI CCWGG 5 cut(s) 582, 976, 2761, 2776, 3211
PspN4I GGNNCC 9 cut(s) 263, 370, 1198, 1971, 2775, 2806, 2971, 3238, 3290
PspPI GGNCC 5 cut(s) 1969, 2805, 2950, 2969, 3236
PspPPI RGGWCCY 2 cut(s) 2805, 2950
PsuI RGATCY 5 cut(s) 463, 1914, 2347, 2847, 2961
RsaI GTAC 6 cut(s) 46, 792, 1044, 1737, 2582, 3221
RsaNI GTAC 6 cut(s) 45, 791, 1043, 1736, 2581, 3220
RseI CAYNNNNRTG 5 cut(s) 108, 1935, 2108, 2261, 2672
SacI GAGCTC 1 cut(s) 1086
SacII CCGCGG 1 cut(s) 3295
SapI GCTCTTC 2 cut(s) 1188, 2093
Sau96I GGNCC 5 cut(s) 1969, 2805, 2950, 2969, 3236
ScaI AGTACT 2 cut(s) 46, 2582
SchI GAGTC 2 cut(s) 1802, 3302
ScrFI CCNGG 6 cut(s) 584, 978, 2534, 2763, 2778, 3213
SduI GDGCHC 5 cut(s) 504, 1086, 2406, 3126, 3152
SexAI ACCWGGT 1 cut(s) 582
SfcI CTRYAG 1 cut(s) 1861
Sfr274I CTCGAG 1 cut(s) 600
Sfr303I CCGCGG 1 cut(s) 3295
SfuI TTCGAA 3 cut(s) 136, 236, 2432
SgrBI CCGCGG 1 cut(s) 3295
SinI GGWCC 4 cut(s) 1969, 2805, 2950, 3236
SlaI CTCGAG 1 cut(s) 600
SmiMI CAYNNNNRTG 5 cut(s) 108, 1935, 2108, 2261, 2672
SmlI CTYRAG 9 cut(s) 61, 161, 600, 1538, 1585, 1870, 2114, 2843, 2954
SmoI CTYRAG 9 cut(s) 61, 161, 600, 1538, 1585, 1870, 2114, 2843, 2954
SphI GCATGC 1 cut(s) 1100
SseBI AGGCCT 3 cut(s) 132, 302, 2394
SsiI CCGC 9 cut(s) 1164, 1401, 3108, 3292, 3294, 3359, 3420, 3423, 3459
SspI AATATT 1 cut(s) 2083
SstI GAGCTC 1 cut(s) 1086
StuI AGGCCT 3 cut(s) 132, 302, 2394
StyD4I CCNGG 6 cut(s) 582, 976, 2532, 2761, 2776, 3211
StyI CCWWGG 7 cut(s) 1686, 1803, 2169, 2309, 2496, 2851, 3240
TaaI ACNGT 4 cut(s) 1417, 1456, 2482, 3067
TaiI ACGT 4 cut(s) 57, 1549, 3085, 3221
TaqII GACCGA 2 cut(s) 1455, 1957
TatI WGTACW 3 cut(s) 44, 1735, 2580
TauI GCSGC 5 cut(s) 1404, 3111, 3297, 3361, 3423
TscAI CASTG 6 cut(s) 130, 330, 1441, 2127, 2432, 3029
TseFI GTSAC 4 cut(s) 1268, 1436, 2118, 3061
TseI GCWGC 7 cut(s) 190, 443, 1866, 3340, 3377, 3380, 3435
Tsp45I GTSAC 4 cut(s) 1268, 1436, 2118, 3061
TspGWI ACGGA 5 cut(s) 307, 2294, 2702, 2860, 3061
TspRI CASTG 6 cut(s) 130, 330, 1441, 2127, 2432, 3029
Van91I CCANNNNNTGG 2 cut(s) 1124, 2996
Vha464I CTTAAG 3 cut(s) 1870, 2114, 2954
VneI GTGCAC 1 cut(s) 3122
VpaK11BI GGWCC 4 cut(s) 1969, 2805, 2950, 3236
XapI RAATTY 9 cut(s) 718, 765, 1216, 1327, 1760, 1796, 2217, 2881, 3203
XbaI TCTAGA 3 cut(s) 389, 1978, 2041
XceI RCATGY 4 cut(s) 314, 1100, 1350, 1636
XcmI CCANNNNNNNNNTGG 2 cut(s) 1121, 2030
XhoI CTCGAG 1 cut(s) 600
XmiI GTMKAC 1 cut(s) 1644
XmnI GAANNNNTTC 5 cut(s) 1257, 1515, 1761, 2864, 3207
ZrmI AGTACT 2 cut(s) 46, 2582
Zsp2I ATGCAT 3 cut(s) 1485, 1638, 2550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.