Rw1G017980

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000036
Physical Location & Seq
Forward (+)
39662982 .. 39667867
4886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G017980.1

Sequence Viewer

Length: 3630 bp
ATGGATGCTGCTAGCTCATCATCATCAGCTCAACAAGCTTCGTCTTCCAGTATTCCTCCTACTCAATTCACATATCAAGTGTTCTTGAGTTTCAGAGGCAGAAACACTCGCAAGAATTTCACCGATCATCTCTACACAGCCCTGACCACTGCAGGAATCTGCACTTTCCGAGACGACGATGAGCTCCCTGCCGGGGAAGCCATCAAGCCAGAACTAGAGAGAGCCATCCAACACTCGCGATGTTCTGTTATTGTGTTTTCCAAAGACTACGCGTCGTCCGGATGGTGCCTGGACGAGCTTGTGACGATCCTTCGATGCAAGAAGAGGTCCGGTCAGTTCGTTTTACCGATCTACTACGACGTCAGCCCATCCCAGGTGAGGAAGCGGACGGGGAGTCTCGCAAAGGCGTTCGTCACACACCAGAAGAATCAAACGGCGGAAAAGCTGATTCTGTGGAAGGAAGCACTTACAGAAGTTGCGGCCCTAGGAGGCATGGTGTTGAAGGATCAAGTTGACGGGCACGAGTCAAAATTTATTGAAAATATTGTTAAGGTGATTGAAGGCAAGTTATGTCGAGTTCCCTTATGTGTGGCCCTTTACCTGGTTGGAATTGATTCTCGAGTGAAAAGCATCAATTTATGGTTACAGGATGGATCGTCCGATATTGGTATATTAGGAATTTGTGGAATTGGTGGAATAGGAAAGACAACTATTGCACAAATTGTTTATAATTCAAATTTTGAGAGATTTGAAAGAAGCTGCTTTCTTGAAAATATCAGAGAGATTTTCGAACGGCCAAGTGGTTTTGTTCAAGTACAGAAACAGCTTCTTTCGGATATTTTGAATGGGAGAAAAGTGAATATAAGCAGTCCTAGCGAGGGAATGAGTAAAATAAAAGATGCCATAAGCTCTAAAAAAGTTCTTCTTGTTCTCGATGATGTGGATCACAAGGACCTAGTTGATGCAATACTTGAGATGAAAGGCTGCTTTTGTTCTGAAAGTAAAATTATAATAACAACAAGGAATGCAGGATTGCTCAAGGGTCATCGAGATATTAAGGTGCATAATGTCGAAGCTTTGAACCATATTGAATCATTGGACCTCTTCAGCAGGCATGCTTTTGGACACAACCACCCTATTGAAAGTTACGTGAAACTTTCAGACAGGGTAGTGCACCACTGTGAAGGACTTCCATTAGCCCTTCAAACATTGGGTTCTTCTCTCTCAGGGAAGACTATAGATGTATGGGAAAGTACATTAAAGAAATCCAAAACTATTCCAAACAGTGTAGTGATGAGTAAACTGAGGATGAGCTTCAACTCTTTACAAGATGACCATGATCAGAGTTTATTCCTCCATATTGCATGTTTTTTTATTGGAAAGGACAAGGATATCATTGTCAAAATTTTAGATGCATGTGATTTCTGCACAACCGTTGGCATTCAAAATCTCATTGATAGATATCTGGTTAGAATTGAAGAATGTAATAAGGTGAATATGCATCACATGATTTGTGACATGGGAAGAGAAATGGTTCGTCTAGAATCAAAAGATCCTAAGAAACGTAGTAGGCTATGGCATCACAAGGACTCTCTTAATGCATTAAGAGAAAAGACTGGTTCTAATGCAATTCAAGGCCTTGTCCTGAACATGCCTGTGAACCCTGCACATAGTCCTTCAAGAAATTCAGATATGGTGGTCTTGGATACCAATGCATTTTCAATGATGAACAAACTCAGATTCCTTCATCTTGGTCATGTACAACTCAATGGAAGTTATGAAAAATTTCCTAAAGGATTGAGATGGTTGTGTTGGCACGAATGTCCTTTGAATTTAATACCGAGTGATTTTCCTATGGAGATGATGGTTGTTATTGAAATGTCCTATAGTAGATTGAGACAAGTGTGGAAGGGAGCAAAGTGTCTTCCATCATTGAAGATCTTTGATCTCAGCCATTCCTCAAACCTTACTGAAACTGGTGACTTCTCATTGGTCCAGAATCTGGAGAGACTTATACTTGAACATTGTATAAGCTTAGTTGATGTCCATGAATCCATAGGAAAGCTAGAGAAACTTGTTTACTTGAATTTGAAAGATTGCATAAATATCATGCAGCTTCCAGAGAACATTCATATGTTAAAATCACTTGAAACACTTATTATATCTGGTTGTTCAAATCTTAGTGAGTTTCCGATGGAGATTAGAAACATGGAAACTCTTAAAGTGTTTCAAGCATTTGGAGTTCCAATAGATCAGCTAATCACTATCCCTGGGCAGGTAAATTCATGGCCAAGATTAAGTGTAGATGGTTTTTGGGGTTCCTTTTCTGGCTCTATTGTTGAGTTAAATCTTGGAAGCTGCAATCTTTCTGATGATGCTTTTCCTAGTGATTTTGGTAACTTATCTTCATTGGAGAGTTTGGATCTCAGCTACAATAACTTTTGTACCTTGCCAGATTCAATCAGAGGCCTTTGTAAACTCAAGATGCTTAATCTTCAATCCTGTAGAAGTCTCATGTCAATTACAGGTCTACCACGAGTAAGAGATCTACTGAGTGCAGACCATTGTGTATCCTTGGAAAAAATAACATTTCAATCACTTTCATGCATACCGAAGAGAGTATCCATATATGGTGACTCCAAACTAGTTGAGATACAGCACTGGTACAAGCTAAAACCCATTGGAACAGTTGATGTAGAAATGATCAGGCTTTTGGGTTTGTCCAACTTGGAATCCATGGAACCCATTCTGATGCACACCGCAGATGCAAACTCCCCGTCGGAAGGGGAATCCATGGAACCCATTCGCATCCAGGGACTACATGAATATGGTATCTTCAGCACATTCTTTCCGGGAAACGGAAATGAGGTTCCAGGACAGTTCAGCCATACAAGTAAAGGGTGTTCCTCAATATCTTTTCCTGTGCCTATACTTCTTAATCACAAGATCCGAGGCTTCAACATATATTCTGTGTATGCATGGATGACAGATGTTACTACTCTATTGCCACCATTAATCATTGAAGTCAATAATAAGAGCACAGGTCTGAAGTGGATCTATGTGCCGTCATGTTACGGCATTCCAAGTGATGGAAAAGACATGATATGGTTAAGCCATTGGAGTTTGGGGAACCAATTCAAAAATGGCGATGTAGTCACTGTCTCAGTATTTACACCAGTTAACTGGCTTCAGGTAAAAGAGTTCGGTGTCCAAGTGGTGCATGAGGAAGAAGAGAAGATGAGTAAGCAAAAGTACAGCAGCAATAGTAGAGATCCTAGTTATGATGATCCCGATGCTTGTGGTGTTAATGAGGTGATTGGTAATAGTGGTGGAGATTTGTCAGAGTTATATCAGGTCAGGCCAGGGACATACTTCCTCTGCGGTGGACCCTTTTTAGAAAATAATAGATACACATATAACAGATGGAAAAAGGATTGTTGCCTCAGTGGCATCATAAGAGAGTATGATGAAGAAACAGACAACGAAGAAATGAAACAGGGGGATAGGACACTAGCAGCAACACAAGAAGCTGAAGCAGAAAGAGGCGGCAGCAATGAATTCTTTGGTTGCAAGAACTTTGTTATCCATGTGCTTATGTCATTGGTTGAGATTTGTCAAAATATCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1209

Amino Acids

135.72

Weight (kDa)

6.2

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 189 3.7e-50 TIR domain
TIR_2 PF13676 27 - 138 1.8e-15 TIR domain
NB-ARC PF00931 207 - 375 2.9e-19 NB-ARC domain
WHD_ROQ1 PF23282 445 - 515 1.3e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 666 - 743 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 781 - 836 1.3e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 729, 1010
AatII GACGTC 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 2266
AccB1I GGYRCC 1 cut(s) 285
AccB7I CCANNNNNTGG 2 cut(s) 2002, 3089
AccI GTMKAC 1 cut(s) 2530
AccII CGCG 2 cut(s) 238, 272
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 6 cut(s) 385, 437, 479, 2760, 3381, 3546
AcoI YGGCCR 2 cut(s) 794, 2288
AcuI CTGAAG 5 cut(s) 1090, 2821, 3068, 3173, 3552
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 6 cut(s) 816, 1255, 1761, 2446, 2666, 3254
AflIII ACRYGT 1 cut(s) 270
AhdI GACNNNNNGTC 2 cut(s) 271, 393
AhlI ACTAGT 1 cut(s) 2644
AjnI CCWGG 7 cut(s) 288, 372, 600, 2269, 2811, 2872, 3361
AleI CACNNNNGTG 1 cut(s) 1179
AloI GAACNNNNNNTCC 2 cut(s) 2853, 2885
Alw21I GWGCWC 3 cut(s) 186, 1176, 3041
Alw26I GTCTC 6 cut(s) 165, 401, 1891, 2002, 2516, 3166
Alw44I GTGCAC 1 cut(s) 1172
AlwNI CAGNNNCTG 1 cut(s) 2002
Ama87I CYCGRG 1 cut(s) 618
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 7 cut(s) 480, 591, 794, 1636, 2288, 2467, 3359
ApaLI GTGCAC 1 cut(s) 1172
ApeKI GCWGC 8 cut(s) 8, 759, 984, 2113, 2358, 3258, 3515, 3549
ArsI GACNNNNNNTTYG 2 cut(s) 2762, 2794
AseI ATTAAT 1 cut(s) 3014
Asp700I GAANNNNTTC 7 cut(s) 613, 1188, 1533, 1785, 2745, 2802, 3134
AspA2I CCTAGG 1 cut(s) 484
AspS9I GGNCC 7 cut(s) 327, 481, 592, 952, 1099, 1993, 3386
AsuC2I CCSGG 2 cut(s) 193, 2853
AsuHPI GGTGA 7 cut(s) 112, 388, 565, 1504, 1991, 2645, 3325
AsuII TTCGAA 1 cut(s) 789
AsuNHI GCTAGC 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 618
AvaII GGWCC 5 cut(s) 327, 952, 1099, 1993, 3386
AvrII CCTAGG 1 cut(s) 484
BaeGI GKGCMC 2 cut(s) 522, 1176
BaeI ACNNNNGTAYC 2 cut(s) 2428, 2461
BalI TGGCCA 1 cut(s) 2290
BanI GGYRCC 1 cut(s) 285
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 2389, 2421
BauI CACGAG 2 cut(s) 521, 2535
BbsI GAAGAC 3 cut(s) 36, 1238, 1916
Bbv12I GWGCWC 3 cut(s) 186, 1176, 3041
BbvI GCAGC 7 cut(s) 746, 971, 2125, 2345, 3270, 3527, 3561
BceAI ACGGC 4 cut(s) 450, 809, 3049, 3091
BciT130I CCWGG 7 cut(s) 290, 374, 602, 2271, 2813, 2874, 3363
BciVI GTATCC 3 cut(s) 1699, 2581, 2632
BclI TGATCA 2 cut(s) 1339, 2703
BcnI CCSGG 2 cut(s) 193, 2853
BcoDI GTCTC 6 cut(s) 165, 401, 1891, 2002, 2516, 3166
BcuI ACTAGT 1 cut(s) 2644
BfmI CTRYAG 4 cut(s) 150, 1236, 1885, 2503
BfuAI ACCTGC 1 cut(s) 2266
BfuI GTATCC 3 cut(s) 1699, 2581, 2632
BglI GCCNNNNNGGC 1 cut(s) 3447
BglII AGATCT 2 cut(s) 1938, 2545
BlnI CCTAGG 1 cut(s) 484
Bme1390I CCNGG 9 cut(s) 193, 290, 374, 602, 2271, 2813, 2853, 2874, 3363
Bme18I GGWCC 5 cut(s) 327, 952, 1099, 1993, 3386
BmeRI GACNNNNNGTC 2 cut(s) 271, 393
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 7 cut(s) 327, 481, 592, 952, 1099, 1993, 3386
BmiI GGNNCC 7 cut(s) 287, 2320, 2742, 2799, 2871, 3131, 3388
BmrFI CCNGG 9 cut(s) 193, 290, 374, 602, 2271, 2813, 2853, 2874, 3363
BmtI GCTAGC 1 cut(s) 15
BpiI GAAGAC 3 cut(s) 36, 1238, 1916
BpmI CTGGAG 1 cut(s) 2024
Bpu14I TTCGAA 1 cut(s) 789
BpuEI CTTGAG 4 cut(s) 106, 992, 1022, 2465
BpuMI CCSGG 2 cut(s) 193, 2853
BsaAI YACGTR 1 cut(s) 1150
BsaBI GATNNNNATC 2 cut(s) 942, 1461
BsaHI GRCGYC 1 cut(s) 360
BsaWI WCCGGW 2 cut(s) 278, 329
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bse1I ACTGG 6 cut(s) 48, 1621, 1981, 2666, 3176, 3188
Bse3DI GCAATG 1 cut(s) 3559
Bse8I GATNNNNATC 2 cut(s) 942, 1461
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 7 cut(s) 290, 374, 602, 2271, 2813, 2874, 3363
BseGI GGATG 8 cut(s) 10, 225, 287, 368, 655, 1314, 2808, 2988
BseJI GATNNNNATC 2 cut(s) 942, 1461
BseMI GCAATG 1 cut(s) 3559
BseMII CTCAG 8 cut(s) 1239, 1295, 1750, 1963, 2440, 2543, 3177, 3457
BseNI ACTGG 6 cut(s) 48, 1621, 1981, 2666, 3176, 3188
BseSI GKGCMC 2 cut(s) 522, 1176
BseXI GCAGC 7 cut(s) 746, 971, 2125, 2345, 3270, 3527, 3561
BsgI GTGCAG 4 cut(s) 145, 1411, 1650, 2577
Bsh1236I CGCG 2 cut(s) 238, 272
BshFI GGCC 7 cut(s) 482, 593, 796, 1638, 2290, 2469, 3361
BshNI GGYRCC 1 cut(s) 285
BsiHKAI GWGCWC 3 cut(s) 186, 1176, 3041
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 4 cut(s) 192, 279, 330, 2852
BslFI GGGAC 2 cut(s) 2829, 3379
BsmAI GTCTC 6 cut(s) 165, 401, 1891, 2002, 2516, 3166
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 2 cut(s) 2829, 3379
BsmI GAATGC 3 cut(s) 1030, 1440, 3078
BsnI GGCC 7 cut(s) 482, 593, 796, 1638, 2290, 2469, 3361
BsoBI CYCGRG 1 cut(s) 618
Bsp119I TTCGAA 1 cut(s) 789
Bsp1286I GDGCHC 4 cut(s) 186, 522, 1176, 3041
Bsp13I TCCGGA 1 cut(s) 278
Bsp1407I TGTACA 1 cut(s) 1759
Bsp19I CCATGG 2 cut(s) 2736, 2793
Bsp68I TCGCGA 1 cut(s) 238
BspACI CCGC 6 cut(s) 385, 437, 479, 2760, 3381, 3546
BspANI GGCC 7 cut(s) 482, 593, 796, 1638, 2290, 2469, 3361
BspCNI CTCAG 8 cut(s) 1238, 1296, 1749, 1962, 2439, 2544, 3176, 3456
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 2 cut(s) 238, 272
BspLI GGNNCC 7 cut(s) 287, 2320, 2742, 2799, 2871, 3131, 3388
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 2266
BspOI GCTAGC 1 cut(s) 15
BspT104I TTCGAA 1 cut(s) 789
BspT107I GGYRCC 1 cut(s) 285
BsrDI GCAATG 1 cut(s) 3559
BsrGI TGTACA 1 cut(s) 1759
BsrI ACTGG 6 cut(s) 48, 1621, 1981, 2666, 3176, 3188
BssNI GRCGYC 1 cut(s) 360
BssSI CACGAG 2 cut(s) 521, 2535
BssT1I CCWWGG 4 cut(s) 484, 2574, 2736, 2793
Bst2BI CACGAG 2 cut(s) 521, 2535
Bst2UI CCWGG 7 cut(s) 290, 374, 602, 2271, 2813, 2874, 3363
Bst4CI ACNGT 6 cut(s) 1181, 1286, 1435, 2689, 2880, 3160
Bst6I CTCTTC 5 cut(s) 317, 1109, 1519, 2609, 3225
BstACI GRCGYC 1 cut(s) 360
BstAUI TGTACA 1 cut(s) 1759
BstBAI YACGTR 1 cut(s) 1150
BstBI TTCGAA 1 cut(s) 789
BstC8I GCNNGC 3 cut(s) 13, 1112, 1116
BstDSI CCRYGG 2 cut(s) 2736, 2793
BstF5I GGATG 8 cut(s) 10, 225, 287, 368, 655, 1314, 2808, 2988
BstFNI CGCG 2 cut(s) 238, 272
BstMAI GTCTC 6 cut(s) 165, 401, 1891, 2002, 2516, 3166
BstMWI GCNNNNNNNGC 4 cut(s) 35, 197, 873, 3447
BstNI CCWGG 7 cut(s) 290, 374, 602, 2271, 2813, 2874, 3363
BstNSI RCATGY 4 cut(s) 1118, 1368, 1419, 1654
BstSCI CCNGG 9 cut(s) 191, 288, 372, 600, 2269, 2811, 2851, 2872, 3361
BstSFI CTRYAG 4 cut(s) 150, 1236, 1885, 2503
BstSLI GKGCMC 2 cut(s) 522, 1176
BstUI CGCG 2 cut(s) 238, 272
BstV1I GCAGC 7 cut(s) 746, 971, 2125, 2345, 3270, 3527, 3561
BstV2I GAAGAC 3 cut(s) 36, 1238, 1916
BstX2I RGATCY 7 cut(s) 1552, 1938, 2422, 2545, 2946, 3054, 3271
BstXI CCANNNNNNTGG 1 cut(s) 3183
BstYI RGATCY 7 cut(s) 1552, 1938, 2422, 2545, 2946, 3054, 3271
BsuI GTATCC 3 cut(s) 1699, 2581, 2632
BsuRI GGCC 7 cut(s) 482, 593, 796, 1638, 2290, 2469, 3361
BtgI CCRYGG 2 cut(s) 2736, 2793
BtgZI GCGATG 2 cut(s) 253, 3162
BtsCI GGATG 8 cut(s) 10, 225, 287, 368, 655, 1314, 2808, 2988
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 6 cut(s) 147, 1177, 1291, 2659, 3156, 3451
BtuMI TCGCGA 1 cut(s) 238
BveI ACCTGC 1 cut(s) 2266
Cac8I GCNNGC 3 cut(s) 13, 1112, 1116
CaiI CAGNNNCTG 1 cut(s) 2002
Cfr13I GGNCC 7 cut(s) 327, 481, 592, 952, 1099, 1993, 3386
CseI GACGC 1 cut(s) 261
CsiI ACCWGGT 1 cut(s) 600
Csp6I GTAC 6 cut(s) 815, 1254, 1760, 2445, 2665, 3253
CviQI GTAC 6 cut(s) 815, 1254, 1760, 2445, 2665, 3253
DriI GACNNNNNGTC 2 cut(s) 271, 393
EaeI YGGCCR 2 cut(s) 794, 2288
Eam1104I CTCTTC 5 cut(s) 317, 1109, 1519, 2609, 3225
Eam1105I GACNNNNNGTC 2 cut(s) 271, 393
EarI CTCTTC 5 cut(s) 317, 1109, 1519, 2609, 3225
EciI GGCGGA 1 cut(s) 452
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 4 cut(s) 484, 2574, 2736, 2793
Eco147I AGGCCT 2 cut(s) 1638, 2469
Eco24I GRGCYC 1 cut(s) 186
Eco32I GATATC 2 cut(s) 1393, 1463
Eco47I GGWCC 5 cut(s) 327, 952, 1099, 1993, 3386
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 5 cut(s) 1090, 2821, 3068, 3173, 3552
Eco88I CYCGRG 1 cut(s) 618
EcoICRI GAGCTC 1 cut(s) 184
EcoO109I RGGNCCY 1 cut(s) 952
EcoRI GAATTC 1 cut(s) 3557
EcoRII CCWGG 7 cut(s) 288, 372, 600, 2269, 2811, 2872, 3361
EcoRV GATATC 2 cut(s) 1393, 1463
EcoT14I CCWWGG 4 cut(s) 484, 2574, 2736, 2793
EcoT22I ATGCAT 6 cut(s) 1417, 1503, 1603, 1717, 2609, 2980
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 4 cut(s) 484, 2574, 2736, 2793
Esp3I CGTCTC 1 cut(s) 165
FalI AAGNNNNNCTT 2 cut(s) 909, 941
FaqI GGGAC 2 cut(s) 2829, 3379
FauNDI CATATG 1 cut(s) 2133
FbaI TGATCA 2 cut(s) 1339, 2703
FblI GTMKAC 1 cut(s) 2530
FokI GGATG 8 cut(s) 17, 212, 294, 355, 662, 1321, 2795, 2995
FriOI GRGCYC 1 cut(s) 186
GsuI CTGGAG 1 cut(s) 2024
HaeIII GGCC 7 cut(s) 482, 593, 796, 1638, 2290, 2469, 3361
HapII CCGG 4 cut(s) 192, 279, 330, 2852
HgaI GACGC 1 cut(s) 261
Hin1I GRCGYC 1 cut(s) 360
HincII GTYRAC 2 cut(s) 514, 3181
HindII GTYRAC 2 cut(s) 514, 3181
HindIII AAGCTT 3 cut(s) 36, 1074, 2032
HpaI GTTAAC 1 cut(s) 3181
HpaII CCGG 4 cut(s) 192, 279, 330, 2852
HphI GGTGA 7 cut(s) 112, 388, 565, 1504, 1991, 2645, 3325
Hpy166II GTNNAC 9 cut(s) 514, 1174, 1301, 1660, 2080, 2477, 2531, 3181, 3386
Hpy8I GTNNAC 9 cut(s) 514, 1174, 1301, 1660, 2080, 2477, 2531, 3181, 3386
Hpy99I CGWCG 4 cut(s) 179, 277, 362, 2782
HpyCH4III ACNGT 6 cut(s) 1181, 1286, 1435, 2689, 2880, 3160
HpyCH4IV ACGT 3 cut(s) 360, 1149, 1564
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 197, 873, 3447
HpySE526I ACGT 3 cut(s) 360, 1149, 1564
Hsp92I GRCGYC 1 cut(s) 360
Kpn2I TCCGGA 1 cut(s) 278
Ksp22I TGATCA 2 cut(s) 1339, 2703
KspAI GTTAAC 1 cut(s) 3181
LmnI GCTCC 2 cut(s) 189, 1913
Lsp1109I GCAGC 7 cut(s) 746, 971, 2125, 2345, 3270, 3527, 3561
MabI ACCWGGT 1 cut(s) 600
MaeII ACGT 3 cut(s) 360, 1149, 1564
MflI RGATCY 7 cut(s) 1552, 1938, 2422, 2545, 2946, 3054, 3271
MhlI GDGCHC 4 cut(s) 186, 522, 1176, 3041
MlsI TGGCCA 1 cut(s) 2290
MluI ACGCGT 1 cut(s) 270
MluNI TGGCCA 1 cut(s) 2290
MlyI GAGTC 4 cut(s) 403, 533, 1583, 2630
MmeI TCCRAC 4 cut(s) 253, 586, 2748, 2760
Mox20I TGGCCA 1 cut(s) 2290
Mph1103I ATGCAT 6 cut(s) 1417, 1503, 1603, 1717, 2609, 2980
MroI TCCGGA 1 cut(s) 278
MroXI GAANNNNTTC 7 cut(s) 613, 1188, 1533, 1785, 2745, 2802, 3134
MscI TGGCCA 1 cut(s) 2290
MslI CAYNNNNRTG 6 cut(s) 1179, 1655, 2132, 2602, 2750, 2826
Msp20I TGGCCA 1 cut(s) 2290
MspI CCGG 4 cut(s) 192, 279, 330, 2852
MspR9I CCNGG 9 cut(s) 193, 290, 374, 602, 2271, 2813, 2853, 2874, 3363
Mva1269I GAATGC 3 cut(s) 1030, 1440, 3078
MvaI CCWGG 7 cut(s) 290, 374, 602, 2271, 2813, 2874, 3363
MvnI CGCG 2 cut(s) 238, 272
MwoI GCNNNNNNNGC 4 cut(s) 35, 197, 873, 3447
NciI CCSGG 2 cut(s) 193, 2853
NcoI CCATGG 2 cut(s) 2736, 2793
NdeI CATATG 1 cut(s) 2133
NheI GCTAGC 1 cut(s) 11
NlaIV GGNNCC 7 cut(s) 287, 2320, 2742, 2799, 2871, 3131, 3388
NmuCI GTSAC 6 cut(s) 301, 412, 1514, 1979, 2633, 3154
NruI TCGCGA 1 cut(s) 238
NsiI ATGCAT 6 cut(s) 1417, 1503, 1603, 1717, 2609, 2980
NspI RCATGY 4 cut(s) 1118, 1368, 1419, 1654
NspV TTCGAA 1 cut(s) 789
OliI CACNNNNGTG 1 cut(s) 1179
PaeI GCATGC 1 cut(s) 1118
PaeR7I CTCGAG 1 cut(s) 618
PasI CCCWGGG 1 cut(s) 2270
PceI AGGCCT 2 cut(s) 1638, 2469
PctI GAATGC 3 cut(s) 1030, 1440, 3078
PdmI GAANNNNTTC 7 cut(s) 613, 1188, 1533, 1785, 2745, 2802, 3134
PflMI CCANNNNNTGG 2 cut(s) 2002, 3089
PfoI TCCNGGA 2 cut(s) 2851, 2872
PleI GAGTC 4 cut(s) 402, 532, 1583, 2630
PpsI GAGTC 4 cut(s) 402, 532, 1583, 2630
Ppu21I YACGTR 1 cut(s) 1150
PpuMI RGGWCCY 1 cut(s) 952
PshBI ATTAAT 1 cut(s) 3014
PsiI TTATAA 2 cut(s) 729, 1010
Psp124BI GAGCTC 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 952
Psp6I CCWGG 7 cut(s) 288, 372, 600, 2269, 2811, 2872, 3361
PspGI CCWGG 7 cut(s) 288, 372, 600, 2269, 2811, 2872, 3361
PspN4I GGNNCC 7 cut(s) 287, 2320, 2742, 2799, 2871, 3131, 3388
PspPI GGNCC 7 cut(s) 327, 481, 592, 952, 1099, 1993, 3386
PspPPI RGGWCCY 1 cut(s) 952
PsrI GAACNNNNNNTAC 2 cut(s) 2887, 2919
PstI CTGCAG 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 2002
PsuI RGATCY 7 cut(s) 1552, 1938, 2422, 2545, 2946, 3054, 3271
RruI TCGCGA 1 cut(s) 238
RsaI GTAC 6 cut(s) 816, 1255, 1761, 2446, 2666, 3254
RsaNI GTAC 6 cut(s) 815, 1254, 1760, 2445, 2665, 3253
RseI CAYNNNNRTG 6 cut(s) 1179, 1655, 2132, 2602, 2750, 2826
SacI GAGCTC 1 cut(s) 186
Sau96I GGNCC 7 cut(s) 327, 481, 592, 952, 1099, 1993, 3386
SchI GAGTC 4 cut(s) 403, 533, 1583, 2630
ScrFI CCNGG 9 cut(s) 193, 290, 374, 602, 2271, 2813, 2853, 2874, 3363
SduI GDGCHC 4 cut(s) 186, 522, 1176, 3041
SexAI ACCWGGT 1 cut(s) 600
SfcI CTRYAG 4 cut(s) 150, 1236, 1885, 2503
Sfr274I CTCGAG 1 cut(s) 618
SfuI TTCGAA 1 cut(s) 789
SinI GGWCC 5 cut(s) 327, 952, 1099, 1993, 3386
SlaI CTCGAG 1 cut(s) 618
SmiMI CAYNNNNRTG 6 cut(s) 1179, 1655, 2132, 2602, 2750, 2826
SmlI CTYRAG 5 cut(s) 85, 618, 971, 1037, 2480
SmoI CTYRAG 5 cut(s) 85, 618, 971, 1037, 2480
SpeI ACTAGT 1 cut(s) 2644
SphI GCATGC 1 cut(s) 1118
SseBI AGGCCT 2 cut(s) 1638, 2469
SsiI CCGC 6 cut(s) 385, 437, 479, 2760, 3381, 3546
SspI AATATT 1 cut(s) 544
SstI GAGCTC 1 cut(s) 186
StuI AGGCCT 2 cut(s) 1638, 2469
StyD4I CCNGG 9 cut(s) 191, 288, 372, 600, 2269, 2811, 2851, 2872, 3361
StyI CCWWGG 4 cut(s) 484, 2574, 2736, 2793
TaaI ACNGT 6 cut(s) 1181, 1286, 1435, 2689, 2880, 3160
TaiI ACGT 3 cut(s) 363, 1152, 1567
TaqI TCGA 7 cut(s) 313, 574, 619, 789, 933, 1048, 1071
TatI WGTACW 4 cut(s) 814, 1253, 1759, 3252
TauI GCSGC 2 cut(s) 482, 3549
TscAI CASTG 6 cut(s) 154, 1184, 1291, 2666, 3163, 3451
TseFI GTSAC 6 cut(s) 301, 412, 1514, 1979, 2633, 3154
TseI GCWGC 8 cut(s) 8, 759, 984, 2113, 2358, 3258, 3515, 3549
Tsp45I GTSAC 6 cut(s) 301, 412, 1514, 1979, 2633, 3154
TspGWI ACGGA 1 cut(s) 2874
TspRI CASTG 6 cut(s) 154, 1184, 1291, 2666, 3163, 3451
Van91I CCANNNNNTGG 2 cut(s) 2002, 3089
VneI GTGCAC 1 cut(s) 1172
VpaK11BI GGWCC 5 cut(s) 327, 952, 1099, 1993, 3386
VspI ATTAAT 1 cut(s) 3014
XbaI TCTAGA 1 cut(s) 1540
XceI RCATGY 4 cut(s) 1118, 1368, 1419, 1654
XcmI CCANNNNNNNNNTGG 1 cut(s) 3140
XhoI CTCGAG 1 cut(s) 618
XmaJI CCTAGG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 2530
XmnI GAANNNNTTC 7 cut(s) 613, 1188, 1533, 1785, 2745, 2802, 3134
ZraI GACGTC 1 cut(s) 361
Zsp2I ATGCAT 6 cut(s) 1417, 1503, 1603, 1717, 2609, 2980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.