MD12G1193200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000036
Physical Location & Seq
Reverse (-)
27490404 .. 27495034
4631 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1193200.v1.1.491

Sequence Viewer

Length: 3522 bp
ATGGCTCTTGTGAGAGCAGCTCAAGGAACATCCTCTGATTCCAACACTCGTCGGGGTTACCGTTACGATGTTTTCTTGAGTTTCAGAGGCGAAGACACTCGCAGGAATTTTACCGACCACCTATACACAGCCTTGAACAACGCAGGATATCTAACGTTTCGAGATAACGACGAGCTTGAGAGAGGAGAAGATATCAAGCCAGGACTACAGAAAGCAATCCGGCTGTCACGAACATCTGTTGTCGTGTTTTCGAAATATTACGCGTCATCCAAATGGTGCCTTGATGAGCTTGTCATGATCCTTGAACGCAAGAGGACTACCTCGGATCATGTAGTATTACCAGTCTTCTACGATATCGATCCATCCCATGTGAGGAAGCAGACGGGAAGCATTGGAAAAGCATTTGCTAGACACGAGAAAACTCAGTTGCCGAATACGGTGAAGCGATGGAGGAAGGCTCTTGCAGAGGTTGCTGATCTAGCAGGGATGGTCTTACCAAATGAAGCTGATGGGTACGAGTCAAAGTTTATCACAAAAATTGTTAAGGTGATTGGTGACAAGCTAATTCGCACACCCTTGAGTGTTCAATCCAACTTGATCGGAATCCAATCTCGAGTCAAACGCATCAATCTATGGTTACAAGATGGATCTGATGATGTTGGTATAGTTACTGTGTATGGGATGTCTGGAATAGGGAAGACAACCATTGCTAAATGTGTATACAATTCAAATTTCACAAGTTTTGAAGGAAGCAGCTTTGTTGAAAATATCAGAGAAACGGCAAATCAACCGAATGGGTTAGTTCAAATACAAATGCAAGTTCTTTATGATATTTTGAAGGGGAAAAAGGAGAAAGTGCACAACGTCAGCGAAGGAATAAGTAAGATTGAAAGAGCCATATGCTCTAGAAGAGTTCTTCTTGTTCTTGATGATGTAGACCGTATGGACCAATTAGATGCATTACTACGGATGAAAGATCGGTTTTATCCCGGAAGTAAAATAATCATAACAACAAGGCGTGAAAGGTTGCTAAAGGCACTTCAAGTTACTATGGTGCATGAAGTTGGAACTTTGGGTTACGATGAATCATTAGAGCTCTTCTGCTGGCATGCTTTTGGCCAGGACCATCCCCGTGAAGGTTACATGGAATATTCAAAAGAGCTAGTGCAACATTGTGGAGGACTTCCATTAGCTCTTCAAGTTTTGGGTTCTTCTCTGTTAGGTGAAAGTGTGGGTGTGTGGAAAAGTGCATTGGAGAAGCTAAAAGTTATTCCTAATGATGAAATCATGAATAAACTGAGAATAAGCTATGATAGTTTACAAGATAACCATGACCGGCAATTATTCCTCCACATTGCTTGTTTCCTAGTAGGAAGGGACAAAAACTACATTGTTAGAATACTAGATGGATGTGATTTCTTTACAAATGTTGGCATTCAAAATCTCATTGATAGATCCTTGGTGAAAATTGAAGCCGACAAGGTGAAAATGCACGATCTGATTCGTGATATGGGAAGAGAAATAGTTCGCCTAGAATCAGAAGAGCCTGAGAAACGTAGTAGACTATGGCGCCATAAGGATTCTTTCGAAGTATTGAGGGAAAAGACGGGTACCCAAACAATCCAAGGTCTTGTCTTGGATATGCATAGGCATCCTGCAAACAGTCCAATAAACACAAATGTGACTGTCTTGGAAACCAATGCATTTGAAAGGATGCACAAATTGCGACTACTCCATCTTAGAAGCGTACGGCTGGAGGGATGTTATGCAGATTTCCCTACAAGATTAAGATGGTTGTGTTGGCTTGAATTTCCTTTGGATTCTATACCAATTGATTTTCCTTTGGAGTGTCTAATTGTTCTTGAAATGCAATATAGTAGCTTGAGAGAAGTCTACAAAGGAACAAAATTTCTTCCATCATTGAAGATCCTTGATGTGAGCCATTCTCATGGCCTTAGTCAAACCATGGACTTCTCATACTGCCCCAATCTAGAGGAATTGATTCTTGAAGATTGTGGAAGCCTGCTTGATGTTCATGAATCCATTGGAAACCTAGAGAGACTTGTGTACTTGAATCTGAAGAATTGCAAGAACCTTAGGATGCTTCCAAAGAACATGTGCATGCTAAAATCACTTGAGACTCTCATTTTATCTGGGTGCTCAAATCTTGTTGAGATGACGAAGAAGATGGAATCTTTAAAAATGCTCGAAACAGATGGAATTCCCAGAAGTGAATTATGGCCTGGAAGAAGTTCAAGTATCTTGACTTATTTGCCATGTTCTTTAGTAGAGTTAAGTCTTCGTAGCTGCAATCTTTCTGATGATGCCTTTGCTACGGATTTAAGTAATCTATCCTCATTGCAAGTACTATATTTAGATGATAATCCAATTTCTAGCCTACCTGTTTTCATCAAAGGTTTGACGAAGCTCTGGTTACTCTCTTTTGATAACTGTGAGAGGCTCGAATCACTTGTGGGGTTGCCAAAAATAACACACATGGATGTATATGGTTGCATATCACTAAAACAAATAACCTATCAATCCTATGGGTTGAGAGCGTTGACCTATATGGGTGAGAACCGCAATGTACTTGAGGTCGAGTACTATGACAACTTAGAACAAATTGAAAGAGTTGACGTGGAAATGAGGAAACTTTTCAGTTTGCACAACTTGGAGATGAGGAAACTTTTCAGCTTGCAAAATTTTGAAGAAGTTGATGGGGTTTGGAGTCCCGTTCAGGGACTTGATCGACATGGTATATATAGCACATTCTTTTCTGGGAATGAGGTTCCTGGCCGGTTCAGCTATAGAAGTACAGGGTCCTCAATATCTTTTATTGTGCCTTCTCTTTCAAATCATAAAATTCGGGGCTTGAATATCTTTGCTACCTATGCAAACGAGAACAATATGAGTTATTCGGGGAATGATCCATTTATGAGTATTGAAGTGAGCAACAAGAGCAAGGGTATGAAGTGGACCTATGGCCCACGGTTCAGTGGTCCTGGTTGGGGAGAAGATGTGACATGGTTGAGCCATTGGAAAATGGACAATGAAACAATAATATTAGAATGTGGAGATGAAGTGGTTGTTTCCGTAATGACGAGGAGACCTGATGAGCATATTCAGGTAAAGGAGTTTGGGGTTGAGCTTGTGCAAGAGCACCAAGACCAGATGAGTACCCAACACAACACCACTCCAGATCCTAATGATCCATTTGTCATCGGTGGAGATTTGTCACCGCTGGAACACATTTCAGGACTATACGACTTTTGCTGGTTCGAGGAAGCAGAAATGAAAAATGTTGACTTATGTAGGCCGAAATGGTTGAATACCCTCATCATGGACTCAGACAAAGAAGAAGAGCAACAGAACGATCTTGATCACAAAATTGCAGCAGCGAGTGCCAGAGGCAATAACTGCCATCTGAGAGGATGGAGGGGGCTAGTCACTGCTGCCATCTTCCTTCTCACGCTAACTCTAATCGTTCCGCCCTCCATCTCTCGAAAAAAGAAGCGACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1174

Amino Acids

134.08

Weight (kDa)

6.27

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 199 1.5e-51 TIR domain
TIR_2 PF13676 24 - 121 8e-15 TIR domain
NB-ARC PF00931 204 - 373 7.9e-22 NB-ARC domain
WHD_ROQ1 PF23282 443 - 512 1.1e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 775 - 921 2.6e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1610
AccB1I GGYRCC 3 cut(s) 276, 1569, 1610
AccI GTMKAC 4 cut(s) 720, 936, 1561, 1893
AccII CGCG 1 cut(s) 263
AciI CCGC 3 cut(s) 2581, 3239, 3488
AclI AACGTT 1 cut(s) 155
AclWI GGATC 9 cut(s) 292, 333, 353, 655, 1449, 1921, 2921, 3194, 3203
AcoI YGGCCR 2 cut(s) 1117, 2794
AcsI RAATTY 7 cut(s) 106, 730, 1808, 1907, 2220, 2701, 2862
AcuI CTGAAG 1 cut(s) 2099
AcyI GRCGYC 1 cut(s) 1570
AfaI GTAC 9 cut(s) 515, 1612, 1749, 2069, 2367, 2589, 2603, 2815, 3178
AfiI CCNNNNNNNGG 5 cut(s) 372, 1136, 2738, 3008, 3340
AflIII ACRYGT 2 cut(s) 261, 2115
AjiI CACGTC 1 cut(s) 2638
AjnI CCWGG 5 cut(s) 199, 1119, 2242, 2791, 3001
AjuI GAANNNNNNNTTGG 2 cut(s) 1235, 1267
AleI CACNNNNGTG 1 cut(s) 1679
Alw21I GWGCWC 4 cut(s) 861, 1098, 2162, 3162
Alw26I GTCTC 3 cut(s) 2053, 2132, 3100
Alw44I GTGCAC 1 cut(s) 857
AlwI GGATC 9 cut(s) 292, 333, 353, 655, 1449, 1921, 2921, 3194, 3203
Ama87I CYCGRG 1 cut(s) 612
AoxI GGCC 6 cut(s) 1117, 1951, 2240, 2794, 2983, 3314
ApaLI GTGCAC 1 cut(s) 857
ApeKI GCWGC 6 cut(s) 17, 753, 2307, 3392, 3395, 3452
ApoI RAATTY 7 cut(s) 106, 730, 1808, 1907, 2220, 2701, 2862
Asp700I GAANNNNTTC 5 cut(s) 1524, 2001, 2251, 2654, 2687
Asp718I GGTACC 1 cut(s) 1610
AspLEI GCGC 1 cut(s) 1572
AspS9I GGNCC 6 cut(s) 946, 1123, 2820, 2976, 2984, 2999
AsuC2I CCSGG 1 cut(s) 990
AsuHPI GGTGA 8 cut(s) 451, 559, 566, 1235, 1474, 1495, 2585, 3228
AsuII TTCGAA 2 cut(s) 251, 1587
AvaI CYCGRG 1 cut(s) 612
AvaII GGWCC 5 cut(s) 946, 1123, 2820, 2976, 2999
AxyI CCTNAGG 1 cut(s) 2096
BaeGI GKGCMC 1 cut(s) 861
BalI TGGCCA 1 cut(s) 1119
BanI GGYRCC 3 cut(s) 276, 1569, 1610
BanII GRGCYC 1 cut(s) 1098
BauI CACGAG 1 cut(s) 413
BbsI GAAGAC 4 cut(s) 99, 337, 704, 2291
Bbv12I GWGCWC 4 cut(s) 861, 1098, 2162, 3162
BbvI GCAGC 6 cut(s) 29, 765, 2294, 3404, 3407, 3439
BceAI ACGGC 2 cut(s) 795, 1766
BciT130I CCWGG 5 cut(s) 201, 1121, 2244, 2793, 3003
BclI TGATCA 1 cut(s) 3379
BcnI CCSGG 1 cut(s) 990
BcoDI GTCTC 3 cut(s) 2053, 2132, 3100
BfmI CTRYAG 2 cut(s) 206, 2806
BfoI RGCGCY 1 cut(s) 1573
BisI GCNGC 6 cut(s) 18, 754, 2308, 3393, 3396, 3453
BlsI GCNGC 6 cut(s) 19, 755, 2309, 3394, 3397, 3454
BmcAI AGTACT 2 cut(s) 2367, 2603
Bme1390I CCNGG 6 cut(s) 201, 990, 1121, 2244, 2793, 3003
Bme18I GGWCC 5 cut(s) 946, 1123, 2820, 2976, 2999
BmeT110I CYCGRG 1 cut(s) 612
BmgBI CACGTC 1 cut(s) 2638
BmgT120I GGNCC 6 cut(s) 946, 1123, 2820, 2976, 2984, 2999
BmiI GGNNCC 5 cut(s) 278, 1571, 1612, 2790, 2821
BmrFI CCNGG 6 cut(s) 201, 990, 1121, 2244, 2793, 3003
BmsI GCATC 6 cut(s) 633, 946, 1660, 1704, 2091, 2314
BpiI GAAGAC 4 cut(s) 99, 337, 704, 2291
BplI GAGNNNNNCTC 5 cut(s) 36, 442, 474, 1930, 1962
BpmI CTGGAG 2 cut(s) 1775, 3180
Bpu14I TTCGAA 2 cut(s) 251, 1587
BpuEI CTTGAG 7 cut(s) 6, 97, 197, 598, 1903, 2156, 2612
BpuMI CCSGG 1 cut(s) 990
Bsa29I ATCGAT 1 cut(s) 357
BsaBI GATNNNNATC 4 cut(s) 357, 602, 2382, 3378
BsaHI GRCGYC 1 cut(s) 1570
BsaI GGTCTC 1 cut(s) 3100
BsaJI CCNNGG 5 cut(s) 321, 1458, 1624, 1965, 2987
BsaXI ACNNNNNCTCC 4 cut(s) 3003, 3033, 3066, 3096
Bsc4I CCNNNNNNNGG 5 cut(s) 372, 1136, 2738, 3008, 3340
Bse118I RCCGGY 2 cut(s) 1335, 2796
Bse1I ACTGG 1 cut(s) 341
Bse21I CCTNAGG 1 cut(s) 2096
Bse3DI GCAATG 4 cut(s) 705, 1353, 2357, 2590
Bse8I GATNNNNATC 4 cut(s) 357, 602, 2382, 3378
BseBI CCWGG 5 cut(s) 201, 1121, 2244, 2793, 3003
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 5 cut(s) 321, 1458, 1624, 1965, 2987
BseJI GATNNNNATC 4 cut(s) 357, 602, 2382, 3378
BseLI CCNNNNNNNGG 5 cut(s) 372, 1136, 2738, 3008, 3340
BseMI GCAATG 4 cut(s) 705, 1353, 2357, 2590
BseMII CTCAG 5 cut(s) 437, 1289, 1539, 3360, 3416
BseNI ACTGG 1 cut(s) 341
BseRI GAGGAG 2 cut(s) 198, 3118
BseSI GKGCMC 1 cut(s) 861
BseXI GCAGC 6 cut(s) 29, 765, 2294, 3404, 3407, 3439
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 6 cut(s) 1119, 1953, 2242, 2796, 2985, 3316
BshNI GGYRCC 3 cut(s) 276, 1569, 1610
BshVI ATCGAT 1 cut(s) 357
BsiHKAI GWGCWC 4 cut(s) 861, 1098, 2162, 3162
BsiHKCI CYCGRG 1 cut(s) 612
BsiSI CCGG 4 cut(s) 220, 990, 1336, 2797
BsiWI CGTACG 1 cut(s) 1747
BslFI GGGAC 3 cut(s) 1391, 2715, 2754
BslI CCNNNNNNNGG 5 cut(s) 372, 1136, 2738, 3008, 3340
BsmAI GTCTC 3 cut(s) 2053, 2132, 3100
BsmFI GGGAC 3 cut(s) 1391, 2715, 2754
BsmI GAATGC 1 cut(s) 1434
BsnI GGCC 6 cut(s) 1119, 1953, 2242, 2796, 2985, 3316
Bso31I GGTCTC 1 cut(s) 3100
BsoBI CYCGRG 1 cut(s) 612
Bsp119I TTCGAA 2 cut(s) 251, 1587
Bsp1286I GDGCHC 4 cut(s) 861, 1098, 2162, 3162
Bsp19I CCATGG 1 cut(s) 1965
BspACI CCGC 3 cut(s) 2581, 3239, 3488
BspANI GGCC 6 cut(s) 1119, 1953, 2242, 2796, 2985, 3316
BspCNI CTCAG 5 cut(s) 436, 1290, 1540, 3359, 3417
BspDI ATCGAT 1 cut(s) 357
BspFNI CGCG 1 cut(s) 263
BspHI TCATGA 3 cut(s) 294, 1287, 2035
BspLI GGNNCC 5 cut(s) 278, 1571, 1612, 2790, 2821
BspPI GGATC 9 cut(s) 292, 333, 353, 655, 1449, 1921, 2921, 3194, 3203
BspQI GCTCTTC 4 cut(s) 1103, 1200, 1536, 3354
BspT104I TTCGAA 2 cut(s) 251, 1587
BspT107I GGYRCC 3 cut(s) 276, 1569, 1610
BspTNI GGTCTC 1 cut(s) 3100
BsrDI GCAATG 4 cut(s) 705, 1353, 2357, 2590
BsrFI RCCGGY 2 cut(s) 1335, 2796
BsrI ACTGG 1 cut(s) 341
BssAI RCCGGY 2 cut(s) 1335, 2796
BssECI CCNNGG 5 cut(s) 321, 1458, 1624, 1965, 2987
BssNAI GTATAC 1 cut(s) 721
BssNI GRCGYC 1 cut(s) 1570
BssSI CACGAG 1 cut(s) 413
BssT1I CCWWGG 3 cut(s) 1458, 1624, 1965
Bst1107I GTATAC 1 cut(s) 721
Bst2BI CACGAG 1 cut(s) 413
Bst2UI CCWGG 5 cut(s) 201, 1121, 2244, 2793, 3003
Bst4CI ACNGT 9 cut(s) 62, 439, 673, 941, 1664, 1687, 2453, 2991, 3519
Bst6I CTCTTC 6 cut(s) 904, 1103, 1200, 1510, 1536, 3354
BstACI GRCGYC 1 cut(s) 1570
BstAPI GCANNNNNTGC 4 cut(s) 470, 1723, 3401, 3417
BstBI TTCGAA 2 cut(s) 251, 1587
BstC8I GCNNGC 5 cut(s) 1106, 1110, 2024, 2123, 2696
BstDEI CTNAG 9 cut(s) 423, 1298, 1548, 1739, 1955, 2096, 2614, 3346, 3425
BstDSI CCRYGG 2 cut(s) 1965, 2987
BstEII GGTNACC 1 cut(s) 56
BstFNI CGCG 1 cut(s) 263
BstH2I RGCGCY 1 cut(s) 1573
BstHHI GCGC 1 cut(s) 1572
BstMAI GTCTC 3 cut(s) 2053, 2132, 3100
BstMWI GCNNNNNNNGC 8 cut(s) 470, 479, 1723, 2802, 2891, 2958, 3401, 3417
BstNI CCWGG 5 cut(s) 201, 1121, 2244, 2793, 3003
BstNSI RCATGY 3 cut(s) 1112, 2119, 2125
BstPI GGTNACC 1 cut(s) 56
BstSCI CCNGG 6 cut(s) 199, 988, 1119, 2242, 2791, 3001
BstSFI CTRYAG 2 cut(s) 206, 2806
BstSLI GKGCMC 1 cut(s) 861
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 6 cut(s) 29, 765, 2294, 3404, 3407, 3439
BstV2I GAAGAC 4 cut(s) 99, 337, 704, 2291
BstX2I RGATCY 4 cut(s) 647, 1454, 1926, 3199
BstXI CCANNNNNNTGG 1 cut(s) 1949
BstYI RGATCY 4 cut(s) 647, 1454, 1926, 3199
BstZ17I GTATAC 1 cut(s) 721
Bsu15I ATCGAT 1 cut(s) 357
Bsu36I CCTNAGG 1 cut(s) 2096
BsuRI GGCC 6 cut(s) 1119, 1953, 2242, 2796, 2985, 3316
BsuTUI ATCGAT 1 cut(s) 357
BtgI CCRYGG 2 cut(s) 1965, 2987
BtgZI GCGATG 1 cut(s) 460
BtrI CACGTC 1 cut(s) 2638
BtsI GCAGTG 1 cut(s) 3447
BtsIMutI CAGTG 2 cut(s) 3001, 3447
Cac8I GCNNGC 5 cut(s) 1106, 1110, 2024, 2123, 2696
CciI TCATGA 3 cut(s) 294, 1287, 2035
CfoI GCGC 1 cut(s) 1572
Cfr10I RCCGGY 2 cut(s) 1335, 2796
Cfr13I GGNCC 6 cut(s) 946, 1123, 2820, 2976, 2984, 2999
ClaI ATCGAT 1 cut(s) 357
CseI GACGC 1 cut(s) 252
Csp6I GTAC 9 cut(s) 514, 1611, 1748, 2068, 2366, 2588, 2602, 2814, 3177
CspCI CAANNNNNGTGG 2 cut(s) 1340, 1375
CviQI GTAC 9 cut(s) 514, 1611, 1748, 2068, 2366, 2588, 2602, 2814, 3177
DdeI CTNAG 9 cut(s) 423, 1298, 1548, 1739, 1955, 2096, 2614, 3346, 3425
DinI GGCGCC 1 cut(s) 1571
DraI TTTAAA 1 cut(s) 2199
EaeI YGGCCR 2 cut(s) 1117, 2794
Eam1104I CTCTTC 6 cut(s) 904, 1103, 1200, 1510, 1536, 3354
EarI CTCTTC 6 cut(s) 904, 1103, 1200, 1510, 1536, 3354
EciI GGCGGA 1 cut(s) 3477
Ecl136II GAGCTC 1 cut(s) 1096
Eco130I CCWWGG 3 cut(s) 1458, 1624, 1965
Eco24I GRGCYC 1 cut(s) 1098
Eco31I GGTCTC 1 cut(s) 3100
Eco32I GATATC 3 cut(s) 149, 193, 355
Eco47I GGWCC 5 cut(s) 946, 1123, 2820, 2976, 2999
Eco53kI GAGCTC 1 cut(s) 1096
Eco57I CTGAAG 1 cut(s) 2099
Eco81I CCTNAGG 1 cut(s) 2096
Eco88I CYCGRG 1 cut(s) 612
Eco91I GGTNACC 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 1096
EcoO109I RGGNCCY 1 cut(s) 2820
EcoO65I GGTNACC 1 cut(s) 56
EcoRI GAATTC 1 cut(s) 2220
EcoRII CCWGG 5 cut(s) 199, 1119, 2242, 2791, 3001
EcoRV GATATC 3 cut(s) 149, 193, 355
EcoT14I CCWWGG 3 cut(s) 1458, 1624, 1965
EcoT22I ATGCAT 3 cut(s) 961, 1647, 1705
EcoT38I GRGCYC 1 cut(s) 1098
EgeI GGCGCC 1 cut(s) 1571
EheI GGCGCC 1 cut(s) 1571
ErhI CCWWGG 3 cut(s) 1458, 1624, 1965
FaqI GGGAC 3 cut(s) 1391, 2715, 2754
FauNDI CATATG 1 cut(s) 899
FbaI TGATCA 1 cut(s) 3379
FblI GTMKAC 4 cut(s) 720, 936, 1561, 1893
Fnu4HI GCNGC 6 cut(s) 18, 754, 2308, 3393, 3396, 3453
FriOI GRGCYC 1 cut(s) 1098
Fsp4HI GCNGC 6 cut(s) 18, 754, 2308, 3393, 3396, 3453
GlaI GCGC 1 cut(s) 1571
GluI GCNGC 6 cut(s) 18, 754, 2308, 3393, 3396, 3453
GsuI CTGGAG 2 cut(s) 1775, 3180
HaeII RGCGCY 1 cut(s) 1573
HaeIII GGCC 6 cut(s) 1119, 1953, 2242, 2796, 2985, 3316
HapII CCGG 4 cut(s) 220, 990, 1336, 2797
HgaI GACGC 1 cut(s) 252
HhaI GCGC 1 cut(s) 1572
Hin1I GRCGYC 1 cut(s) 1570
Hin6I GCGC 1 cut(s) 1570
HinP1I GCGC 1 cut(s) 1570
HincII GTYRAC 3 cut(s) 2562, 2635, 3304
HindII GTYRAC 3 cut(s) 2562, 2635, 3304
HpaII CCGG 4 cut(s) 220, 990, 1336, 2797
HphI GGTGA 8 cut(s) 451, 559, 566, 1235, 1474, 1495, 2585, 3228
Hpy99I CGWCG 2 cut(s) 54, 173
HpyAV CCTTC 8 cut(s) 448, 740, 832, 866, 1130, 1368, 2853, 3473
HpyCH4III ACNGT 9 cut(s) 62, 439, 673, 941, 1664, 1687, 2453, 2991, 3519
HpyCH4IV ACGT 4 cut(s) 155, 864, 1555, 2637
HpyF10VI GCNNNNNNNGC 8 cut(s) 470, 479, 1723, 2802, 2891, 2958, 3401, 3417
HpyF3I CTNAG 9 cut(s) 423, 1298, 1548, 1739, 1955, 2096, 2614, 3346, 3425
HpySE526I ACGT 4 cut(s) 155, 864, 1555, 2637
Hsp92I GRCGYC 1 cut(s) 1570
HspAI GCGC 1 cut(s) 1570
KasI GGCGCC 1 cut(s) 1569
KpnI GGTACC 1 cut(s) 1614
Ksp22I TGATCA 1 cut(s) 3379
LguI GCTCTTC 4 cut(s) 1103, 1200, 1536, 3354
Lsp1109I GCAGC 6 cut(s) 29, 765, 2294, 3404, 3407, 3439
LweI GCATC 6 cut(s) 633, 946, 1660, 1704, 2091, 2314
MaeII ACGT 4 cut(s) 155, 864, 1555, 2637
MfeI CAATTG 1 cut(s) 1830
MflI RGATCY 4 cut(s) 647, 1454, 1926, 3199
MhlI GDGCHC 4 cut(s) 861, 1098, 2162, 3162
MlsI TGGCCA 1 cut(s) 1119
MluI ACGCGT 1 cut(s) 261
MluNI TGGCCA 1 cut(s) 1119
Mly113I GGCGCC 1 cut(s) 1570
MlyI GAGTC 5 cut(s) 527, 624, 2134, 2737, 3338
MmeI TCCRAC 3 cut(s) 66, 615, 1045
Mox20I TGGCCA 1 cut(s) 1119
Mph1103I ATGCAT 3 cut(s) 961, 1647, 1705
MroXI GAANNNNTTC 5 cut(s) 1524, 2001, 2251, 2654, 2687
MscI TGGCCA 1 cut(s) 1119
MseI TTAA 5 cut(s) 543, 1787, 2198, 2294, 2342
MslI CAYNNNNRTG 6 cut(s) 271, 1131, 1679, 1947, 2120, 2499
Msp20I TGGCCA 1 cut(s) 1119
MspA1I CMGCKG 1 cut(s) 3241
MspI CCGG 4 cut(s) 220, 990, 1336, 2797
MspR9I CCNGG 6 cut(s) 201, 990, 1121, 2244, 2793, 3003
MunI CAATTG 1 cut(s) 1830
Mva1269I GAATGC 1 cut(s) 1434
MvaI CCWGG 5 cut(s) 201, 1121, 2244, 2793, 3003
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 8 cut(s) 470, 479, 1723, 2802, 2891, 2958, 3401, 3417
NarI GGCGCC 1 cut(s) 1570
NciI CCSGG 1 cut(s) 990
NcoI CCATGG 1 cut(s) 1965
NdeI CATATG 1 cut(s) 899
NlaIV GGNNCC 5 cut(s) 278, 1571, 1612, 2790, 2821
NmuCI GTSAC 6 cut(s) 225, 554, 1681, 3019, 3234, 3445
NsiI ATGCAT 3 cut(s) 961, 1647, 1705
NspI RCATGY 3 cut(s) 1112, 2119, 2125
NspV TTCGAA 2 cut(s) 251, 1587
OliI CACNNNNGTG 1 cut(s) 1679
PaeI GCATGC 2 cut(s) 1112, 2125
PaeR7I CTCGAG 1 cut(s) 612
PagI TCATGA 3 cut(s) 294, 1287, 2035
PciI ACATGT 1 cut(s) 2115
PciSI GCTCTTC 4 cut(s) 1103, 1200, 1536, 3354
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PctI GAATGC 1 cut(s) 1434
PdmI GAANNNNTTC 5 cut(s) 1524, 2001, 2251, 2654, 2687
Pfl23II CGTACG 1 cut(s) 1747
PfoI TCCNGGA 1 cut(s) 988
PkrI GCNGC 6 cut(s) 19, 755, 2309, 3394, 3397, 3454
PleI GAGTC 5 cut(s) 526, 623, 2134, 2736, 3338
PluTI GGCGCC 1 cut(s) 1573
PpsI GAGTC 5 cut(s) 526, 623, 2134, 2736, 3338
PpuMI RGGWCCY 1 cut(s) 2820
PscI ACATGT 1 cut(s) 2115
Psp124BI GAGCTC 1 cut(s) 1098
Psp1406I AACGTT 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 2820
Psp6I CCWGG 5 cut(s) 199, 1119, 2242, 2791, 3001
PspEI GGTNACC 1 cut(s) 56
PspGI CCWGG 5 cut(s) 199, 1119, 2242, 2791, 3001
PspLI CGTACG 1 cut(s) 1747
PspN4I GGNNCC 5 cut(s) 278, 1571, 1612, 2790, 2821
PspPI GGNCC 6 cut(s) 946, 1123, 2820, 2976, 2984, 2999
PspPPI RGGWCCY 1 cut(s) 2820
PsuI RGATCY 4 cut(s) 647, 1454, 1926, 3199
RsaI GTAC 9 cut(s) 515, 1612, 1749, 2069, 2367, 2589, 2603, 2815, 3178
RsaNI GTAC 9 cut(s) 514, 1611, 1748, 2068, 2366, 2588, 2602, 2814, 3177
RseI CAYNNNNRTG 6 cut(s) 271, 1131, 1679, 1947, 2120, 2499
SacI GAGCTC 1 cut(s) 1098
SapI GCTCTTC 4 cut(s) 1103, 1200, 1536, 3354
SaqAI TTAA 5 cut(s) 543, 1787, 2198, 2294, 2342
SatI GCNGC 6 cut(s) 18, 754, 2308, 3393, 3396, 3453
Sau96I GGNCC 6 cut(s) 946, 1123, 2820, 2976, 2984, 2999
ScaI AGTACT 2 cut(s) 2367, 2603
SchI GAGTC 5 cut(s) 527, 624, 2134, 2737, 3338
ScrFI CCNGG 6 cut(s) 201, 990, 1121, 2244, 2793, 3003
SduI GDGCHC 4 cut(s) 861, 1098, 2162, 3162
SfaNI GCATC 6 cut(s) 633, 946, 1660, 1704, 2091, 2314
SfcI CTRYAG 2 cut(s) 206, 2806
SfoI GGCGCC 1 cut(s) 1571
Sfr274I CTCGAG 1 cut(s) 612
SfuI TTCGAA 2 cut(s) 251, 1587
SinI GGWCC 5 cut(s) 946, 1123, 2820, 2976, 2999
SlaI CTCGAG 1 cut(s) 612
SmiMI CAYNNNNRTG 6 cut(s) 271, 1131, 1679, 1947, 2120, 2499
SmlI CTYRAG 8 cut(s) 21, 76, 176, 577, 612, 1882, 2135, 2591
SmoI CTYRAG 8 cut(s) 21, 76, 176, 577, 612, 1882, 2135, 2591
SphI GCATGC 2 cut(s) 1112, 2125
SsiI CCGC 3 cut(s) 2581, 3239, 3488
SspDI GGCGCC 1 cut(s) 1569
SspI AATATT 3 cut(s) 257, 1151, 3063
SstI GAGCTC 1 cut(s) 1098
StyD4I CCNGG 6 cut(s) 199, 988, 1119, 2242, 2791, 3001
StyI CCWWGG 3 cut(s) 1458, 1624, 1965
TaaI ACNGT 9 cut(s) 62, 439, 673, 941, 1664, 1687, 2453, 2991, 3519
TaiI ACGT 4 cut(s) 158, 867, 1558, 2640
TatI WGTACW 5 cut(s) 2067, 2365, 2587, 2601, 2813
Tru1I TTAA 5 cut(s) 543, 1787, 2198, 2294, 2342
Tru9I TTAA 5 cut(s) 543, 1787, 2198, 2294, 2342
TscAI CASTG 2 cut(s) 3001, 3454
TseFI GTSAC 6 cut(s) 225, 554, 1681, 3019, 3234, 3445
TseI GCWGC 6 cut(s) 17, 753, 2307, 3392, 3395, 3452
Tsp45I GTSAC 6 cut(s) 225, 554, 1681, 3019, 3234, 3445
TspGWI ACGGA 3 cut(s) 982, 2351, 3082
TspRI CASTG 2 cut(s) 3001, 3454
VneI GTGCAC 1 cut(s) 857
VpaK11BI GGWCC 5 cut(s) 946, 1123, 2820, 2976, 2999
XapI RAATTY 7 cut(s) 106, 730, 1808, 1907, 2220, 2701, 2862
XbaI TCTAGA 2 cut(s) 905, 1990
XceI RCATGY 3 cut(s) 1112, 2119, 2125
XhoI CTCGAG 1 cut(s) 612
XmiI GTMKAC 4 cut(s) 720, 936, 1561, 1893
XmnI GAANNNNTTC 5 cut(s) 1524, 2001, 2251, 2654, 2687
ZrmI AGTACT 2 cut(s) 2367, 2603
Zsp2I ATGCAT 3 cut(s) 961, 1647, 1705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.