Rw6G022290

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000036
Physical Location & Seq
Forward (+)
44084736 .. 44110256
25521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G022290.1

Sequence Viewer

Length: 3513 bp
ATGGCTCTTGCACTTGCAACAACTATCTCTCCGAATACTAGTCCAACTAGTTATCATGTGTTCTTGAGTTTCAGGGGTGATACTCGCAAGACTTTTACTGACCACCTCTACACAGCCTTTGTCAATTCAGGCTTTCGTACGTTTCGAGATGACGACGAACTTCCAAGGGGACATGATATCAAACCAGAACTGCGGAAAGCCATCGAGCAGTCACGAAGTTCTGTTATTGTGTTTTCAAAAGACTATGCGTCTTCCGGATGGTGCCTTGATGAGCTTGCGATGATCATGGAACGCAAGAGAACCCGCGATCACATAGTTTTACCTGTATTCTACGATGTCGATCCATCCCAAGTAAGGAAGCAAACAGGAAGTCTTGCAAAAGCATTTGCTAGACACGAAAAAAATCATTCCTTGAAGGACAAGGTGAAGCGATGGAGGGCGGCACTTACAGAAGTTGCAGATCTAGCAGGAATGGTATTGCAAAATGAAGCCGACGGGCACGAGTCTAAGTTTATCAAGAAAATTGTAAGGGTAATGGAAGATAAACTAAGGCGCAATCCCATAAGTGTTGAACCCCACCTAATTGGAATAGATTCTCAAACGGAAGACATCAATTTATGGTTACAAGATGGATCAGCTGATGTTGGCATACTTTTAATTCATGGGATGCGTGGGATAGGAAAATCAACCATAGCGAAATTTGTTTATAAGTCCAACTTTCAAAAATTTGAGAGATGCAGTTTCCTTGAAAATGTCAGAGAAGTTTCAGACCAATCAAATGGCTTGCTTCGGCTACAAAAACAACTTCTTAATGATATTTTGGGTGCAAGTAAAGTGACAATTCACAATATTAGTGAAGGGATGGCTAAGATTGAAGATGCTATTAGTTGTGAAAGAGTTTTTCTTGTTCTCGATGACGTGGATCATGTGGACCAATTAGCCGCATTACTGAGGATGCAAAATAGGTTTCATCCAGGAAGTAAGATCATCATAACAAGTAGCTGTGCGGGATTGTTGGGAGCTCACTGCCAATTTGCTAAGGTGCATGAGGTTAGAATTCTGGGTGAGGATGATTCATTAGCGCTCTTTAGTTGGCATGCTTTTGGACAGGACCATCCCATTGGAAGTTATACTGATTACTCGAAAAGGGTAATAGATCACTGTGGAGGACTTCCACTAGCTCTTAAAGTTTTAGGTTCTTCTTTATCAGGAAAGAATCTAGTTGTCTGGGAAAGTGCATTGAACAAACTAGAAGCTATTCCAAATGGTGAAATAATGAAGAAACTCAAAATAAGCTATGACTCCTTACAAGATGATCATGACAGAAACTTATTCCTTCATATTGCATTCTTTTTCATCGGAATGGAGAAAGGTATCATTGTTACAATCTTAGATGACTGTGACTTCTTCACAGAGGTTGGCATTCAGAATCTCATTGATAGATGTTTGGTAACCACTGACAAAGATAATAAGTTGGTGCAAATGCATGACATGATTCGTGACATGGGAAGAGAAATTGTTCGTCTAGAATCCAAGGAGCCAGAACGACGTAGTCGAGTATGGCATCATAAGGATGCTTTCAAAATATTGTCCGAAAATATTGGCACCCAAACAATTGAAGGACTTGTTCTCAACATGCAAATGTACCCTTCGAGAAATTCAAGTAAGGTAGAATTGGAAACCAATGCATTTACAAGGATGCACAAACTAAGACTCCTCCAGCTCAGTCATGTGCAGCTCAGTGGACGCTACGAAGAATTTCCTGCAGGACTAAGCTGGCTTTGTTGGATTAAATTTCCTCTACCTTCCTTACCCACAGATTTTCCGTTGGAGAGCCTTGTTGCTCTTGAAATGTGTTATAGTAGTTTGAGACGGCTTTGGAGGGGAACAAAGTATCTCCCATCACTAAAAATCTTAAATCTAAGCCACTCTCATGACTTTACAGAAATCTCTGACTTCTCATGTATCCCTAATCTAGAGAGACTCATTCTTGAAGATTGTGCGAGATTGATAAGTGTTCATGAATCCATTGGAGTCCTGGAGAGGCTTATTTATTTGAGTGTGAAAGATTGCAAAAATCTTAGGATGCTTCCGGAGAACATTTCTATCCTAACCTTCCTTGAAACACTTGTTATCTCTGGTTGTTCAAATCTTAGAGAGTTTCCGACAGAAATGAGGAAGCTGCAATCGTTGAAAGTGTTTCAAGCAGATGGAGTTCCAATACATCGGTTACTAGTCACTACCACCAGGGAGGTCGAACTGTGCTCAAGTTCGATTCAAGAACTTTCTTGGGCTTCTTATCTTCCGCGGAATTTAGTAACCTTGAGTATAAGAAACTGCAATCTTTTGGATGATGATTTTCCTAGTGATGTTGCTAATCTATTGTCGCTGCAAAAATTGGATCTCAGCGGGAACCCAATTTCTAGGCTACCGGATTGCATAAGAGGTGTTACTGGCCTCCGAGACCTGTCTTTGAGCAATTGTACAAAGCTCAAATCACTTCTAAGGCTACCAGAATCACTTCATTTCATTGTGGGCGGGTGCACATCATTGGAAAAAGTAACATTTCAATCTTTTTTTCGATCTGGTTCTGGTTGGGGGTCCAACCATAACCTTGTTGAGTTACAGGGTCATTTCAAGATGGAACCCATTGAAAGAGTTGATAAAGAAATGCTAAAACTTTTGAGTCTAGGCAACCTGGAAACCTTGGAAAACATTTCGATGGTCAATAGTGGATTTTATTCTCAATGCATATGGAAGGGAACAGATCCCGTCCAGGGACTGCACGAAGATGGTATATTCAGCACATTCCTTCCTGGAGACGAAGTTCCAGGCCAGTTCAGCCATAGAAGCAGAGGGTCCTCTGTATCCTTTACTGTGCCTTTACTTCCCAATCTCAATATACGAGGGCTGAATGTCTTCTCGGTCATAGCACAATCCAACAACAATGATTCTGATCCTATGACCAATAGTGTCTATATTGACGGTTTAGGTTTTCCGACAATTACCGTTGTGATTAATACGAGCAAGGGGCTGAAGTGGATCCATGGTCCAAACTTCTCTGGCGTTCCGGGTGAAGGAAAAGATGTGATATGGTCAAGTCACTGGAAGTTTGGGAATCTACTTACATGTGGTGATGAGGTGACTATTTTAATATATACGAAATCTGATTTCAAGGTAAAGGAGTGTGGCATCCAGCTTGTTTACTACGATGAGAAAGATCATGTGGCTTCGACCACGGACGATCTTTGTTTTCTCCGAGCTGGAGTTCGGTCAACAATACCCTCTGGGCCGACGGACCGAGGAATAACCTTCCTATGGTCTTACGGAATAGGACTTAGTCCAACTGATGAAAATATTGTCCTCATTAGGGATATCAATGAAAAATCAGTTTATGTGGGAATTGATTTGCTAGTACTATTTCATGTTTATTTTGCAGAGAAAGAAGAAGGGCAAGAGGGTGACCTCGTCCTCACTCTTGCAGCACAAGGTAGCAGCAATAGCAATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1170

Amino Acids

131.67

Weight (kDa)

6.18

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 186 2e-50 TIR domain
TIR_2 PF13676 20 - 116 2.1e-15 TIR domain
NB-ARC PF00931 200 - 369 5.7e-16 NB-ARC domain
AAA_14 PF13173 217 - 339 3.5e-06 AAA domain
WHD_ROQ1 PF23282 440 - 510 2.2e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 646 - 737 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 770 - 828 5.3e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57630 AT1G72930 AT5G38344
fragaria_vesca FvH4_1g27020 FvH4_2g00931 FvH4_2g17330 FvH4_2g17330 FvH4_2g17340 FvH4_2g17350 FvH4_2g20690 FvH4_5g15301 FvH4_5g34194 FvH4_5g38530 FvH4_5g38740 FvH4_5g38740 FvH4_5g38740 FvH4_6g03800 FvH4_7g09791 FvH4_7g09800 FvH4_7g09800 FvH4_7g10510 FvH4_7g10520 FvH4_7g10520 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750 FvH4_7g10750
malus_domestica MD02G1217100.v1.1 MD02G1217200.v1.1 MD02G1217400.v1.1 MD02G1217600.v1.1 MD02G1264800.v1.1 MD02G1264900.v1.1 MD02G1302100.v1.1 MD04G1010700.v1.1 MD05G1006800.v1.1 MD05G1140100.v1.1 MD05G1140200.v1.1 MD05G1152200.v1.1 MD07G1073500.v1.1 MD07G1101700.v1.1 MD07G1102200.v1.1 MD07G1103200.v1.1 MD07G1112300.v1.1 MD08G1018200.v1.1 MD08G1018400.v1.1 MD08G1018800.v1.1 MD08G1019000.v1.1 MD08G1019100.v1.1 MD08G1019200.v1.1 MD08G1019300.v1.1 MD08G1019400.v1.1 MD08G1019600.v1.1 MD08G1019900.v1.1 MD08G1020000.v1.1 MD08G1020200.v1.1 MD08G1020500.v1.1 MD08G1020700.v1.1 MD08G1020800.v1.1 MD08G1040700.v1.1 MD11G1112400.v1.1 MD12G1068600.v1.1 MD12G1193200.v1.1 MD13G1050600.v1.1 MD15G1374900.v1.1 MD15G1375100.v1.1 MD15G1416500.v1.1 MD17G1072500.v1.1 MD17G1274200.v1.1 MD17G1274900.v1.1
prunus_persica Prupe.2G121000_v2.0.a1 Prupe.2G121000_v2.0.a1 Prupe.2G125800_v2.0.a1 Prupe.2G126000_v2.0.a1 Prupe.2G126100_v2.0.a1 Prupe.2G126200_v2.0.a1 Prupe.2G126300_v2.0.a1 Prupe.2G126400_v2.0.a1 Prupe.2G126500_v2.0.a1 Prupe.2G126600_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126700_v2.0.a1 Prupe.2G126800_v2.0.a1 Prupe.2G126900_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G127000_v2.0.a1 Prupe.2G135500_v2.0.a1 Prupe.2G135600_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.2G135700_v2.0.a1 Prupe.3G009800_v2.0.a1 Prupe.3G010200_v2.0.a1 Prupe.3G131000_v2.0.a1 Prupe.4G260800_v2.0.a1 Prupe.4G260900_v2.0.a1 Prupe.6G083100_v2.0.a1 Prupe.6G083200_v2.0.a1 Prupe.6G083400_v2.0.a1 Prupe.6G132100_v2.0.a1 Prupe.8G184900_v2.0.a1 Prupe.8G185200_v2.0.a1 Prupe.8G185300_v2.0.a1
pyrus_communis pycom02g17870 pycom02g17880 pycom02g19520 pycom02g22610 pycom02g22630 pycom02g22680 pycom05g13540 pycom05g14680 pycom07g04880 pycom07g04890 pycom07g05690 pycom07g05710 pycom07g05720 pycom07g05750 pycom07g05780 pycom07g06300 pycom07g08320 pycom07g08330 pycom07g08350 pycom07g08360 pycom07g08370 pycom07g08410 pycom07g08420 pycom07g08430 pycom07g08440 pycom07g08470 pycom07g08480 pycom07g08520 pycom07g08550 pycom07g08560 pycom07g08640 pycom07g08660 pycom07g08670 pycom07g08680 pycom07g08690 pycom07g08720 pycom07g08730 pycom07g08790 pycom07g08800 pycom07g08810 pycom07g08860 pycom07g08890 pycom07g09720 pycom08g01590 pycom08g01600 pycom08g01620 pycom08g01630 pycom08g01640 pycom10g12630 pycom15g16490 pycom17g07200 pycom17g07210 pycom17g07220 pycom17g11730 pycom17g11740 pycom17g27340 pycom17g27380
rosa_chinensis RchiOBHm_Chr1g0342571 RchiOBHm_Chr1g0346231 RchiOBHm_Chr1g0346831 RchiOBHm_Chr1g0346891 RchiOBHm_Chr1g0348311 RchiOBHm_Chr1g0348321 RchiOBHm_Chr1g0348331 RchiOBHm_Chr1g0348361 RchiOBHm_Chr1g0348371 RchiOBHm_Chr1g0348381 RchiOBHm_Chr1g0348401 RchiOBHm_Chr1g0348411 RchiOBHm_Chr1g0348421 RchiOBHm_Chr1g0348431 RchiOBHm_Chr2g0130881 RchiOBHm_Chr3g0487331 RchiOBHm_Chr5g0060371 RchiOBHm_Chr6g0246491 RchiOBHm_Chr6g0246871 RchiOBHm_Chr6g0250311 RchiOBHm_Chr6g0250781 RchiOBHm_Chr6g0251431 RchiOBHm_Chr6g0281871 RchiOBHm_Chr6g0281881 RchiOBHm_Chr6g0281931 RchiOBHm_Chr6g0281941 RchiOBHm_Chr6g0281951 RchiOBHm_Chr6g0281971 RchiOBHm_Chr6g0281981 RchiOBHm_Chr7g0240541 RchiOBHm_Chr7g0240631 RchiOBHm_Chr7g0240671
rosa_laevigata RLG00000000627 RLG00000000631 RLG00000000641 RLG00000000645 RLG00000000652 RLG00000000662 RLG00000000663 RLG00000000667 RLG00000000670 RLG00000000702 RLG00000001216 RLG00000001219 RLG00000007678 RLG00000012923 RLG00000012996 RLG00000013243 RLG00000017513 RLG00000017514 RLG00000020936 RLG00000022755 RLG00000023013 RLG00000028646 RLG00000028654 RLG00000028662 RLG00000028665 RLG00000028667 RLG00000028711 RLG00000028714 RLG00000028716 RLG00000028770 RLG00000028771 RLG00000028773 RLG00000028778 RLG00000028798 RLG00000028809 RLG00000028813 RLG00000029334 RLG00000030003 RLG00000033910 RLG00000034358 RLG00000035430
rosa_multiflora Rmu_co8031134.1_g000001 Rmu_co8092796.1_g000001 Rmu_co8130156.1_g000001 Rmu_co8325323.1_g000001 Rmu_co8424083.1_g000002 Rmu_sc0000008.1_g000003 Rmu_sc0000008.1_g000011 Rmu_sc0000008.1_g000015 Rmu_sc0000008.1_g000020 Rmu_sc0000091.1_g000015 Rmu_sc0000091.1_g000018 Rmu_sc0000091.1_g000029 Rmu_sc0000091.1_g000031 Rmu_sc0000391.1_g000004 Rmu_sc0001070.1_g000030 Rmu_sc0001293.1_g000016 Rmu_sc0001674.1_g000006 Rmu_sc0001731.1_g000002 Rmu_sc0001731.1_g000003 Rmu_sc0001731.1_g000005 Rmu_sc0001911.1_g000029 Rmu_sc0001913.1_g000038 Rmu_sc0001955.1_g000025 Rmu_sc0001992.1_g000022 Rmu_sc0001992.1_g000028 Rmu_sc0001992.1_g000031 Rmu_sc0002517.1_g000013 Rmu_sc0002517.1_g000017 Rmu_sc0002551.1_g000022 Rmu_sc0002551.1_g000029 Rmu_sc0002551.1_g000038 Rmu_sc0002705.1_g000015 Rmu_sc0002705.1_g000016 Rmu_sc0002882.1_g000055 Rmu_sc0003232.1_g000027 Rmu_sc0003363.1_g000037 Rmu_sc0003600.1_g000054 Rmu_sc0003918.1_g000011 Rmu_sc0004395.1_g000011 Rmu_sc0005071.1_g000004 Rmu_sc0005255.1_g000018 Rmu_sc0005327.1_g000004 Rmu_sc0005597.1_g000007 Rmu_sc0007060.1_g000007 Rmu_sc0007310.1_g000003 Rmu_sc0007383.1_g000014 Rmu_sc0007799.1_g000003 Rmu_sc0008477.1_g000016 Rmu_sc0010151.1_g000009 Rmu_sc0011217.1_g000005 Rmu_sc0016269.1_g000006 Rmu_sc0021495.1_g000001 Rmu_sc0027364.1_g000001 Rmu_sc0029432.1_g000001 Rmu_sc0041386.1_g000001 Rmu_ssc0000126.1_g000023 Rmu_ssc0000126.1_g000027
rosa_roxburghii Rroxscaffold_1G00006130 Rroxscaffold_1G00023320 Rroxscaffold_1G00038770 Rroxscaffold_2G00119800 Rroxscaffold_2G00121860 Rroxscaffold_3G00219780 Rroxscaffold_3G00219890 Rroxscaffold_4G00306740 Rroxscaffold_4G00306820 Rroxscaffold_4G00307000 Rroxscaffold_4G00307010 Rroxscaffold_4G00307020 Rroxscaffold_4G00308030 Rroxscaffold_4G00308070 Rroxscaffold_4G00308450 Rroxscaffold_4G00308460 Rroxscaffold_4G00315320 Rroxscaffold_5G00352470 Rroxscaffold_5G00371420 Rroxscaffold_6G00392300 Rroxscaffold_6G00394870 Rroxscaffold_6G00394890 Rroxscaffold_7G00186700 Rroxscaffold_7G00194980 Rroxscaffold_7G00216840
rosa_rugosa Rorug01G0076100 Rorug01G0128100 Rorug01G0149000.1 Rorug01G0149500.1 Rorug01G0180000 Rorug01G0180100 Rorug01G0182900 Rorug01G0184000 Rorug01G0184100 Rorug01G0184300 Rorug01G0184600 Rorug01G0184700 Rorug01G0184700 Rorug01G0184900 Rorug01G0185000 Rorug01G0185100 Rorug01G0189300 Rorug01G0194100 Rorug01G0194100 Rorug01G0194200 Rorug01G0194400 Rorug01G0194400 Rorug01G0194500 Rorug02G0090400 Rorug02G0090400 Rorug03G0233000 Rorug03G0233100 Rorug05G0178600 Rorug05G0178700 Rorug05G0579200 Rorug06G0144100 Rorug06G0144300 Rorug06G0144300 Rorug06G0179300 Rorug06G0208800 Rorug07G0323500 Rorug07G0337000
rosa_samantha Rh1AG046100 Rh1AG201400 Rh1AG201800 Rh1AG202100 Rh1AG202600 Rh1AG211500 Rh1AG211800 Rh1AG212200 Rh1AG212500 Rh1AG212600 Rh1AG214200 Rh1BG168000 Rh1BG168300 Rh1BG168500 Rh1BG177900 Rh1BG178200 Rh1BG178300 Rh1BG178800 Rh1BG178900 Rh1CG080900 Rh1CG183300 Rh1CG186300 Rh1CG186700 Rh1CG187000 Rh1CG187300 Rh1CG193400 Rh1CG195800 Rh1CG196000 Rh1CG196100 Rh1CG196400 Rh1CG196500 Rh1CG196800 Rh1CG198800 Rh1CG442600 Rh1DG086200 Rh1DG155400 Rh1DG156200 Rh1DG194000 Rh1DG194300 Rh1DG194900 Rh1DG197000 Rh1DG198800 Rh1DG199000 Rh1DG199200 Rh1DG202000 Rh1DG203900 Rh1DG207600 Rh1DG207900 Rh1DG208000 Rh1DG208500 Rh1DG208600 Rh1DG208700 Rh3CG339100 Rh5AG106500 Rh5AG264800 Rh5AG265300 Rh5CG302800 Rh5DG277900 Rh6AG257600 Rh6AG257700 Rh6BG260500 Rh6BG260700 Rh6CG259600 Rh6CG259700 Rh6DG252100 Rh7DG126900
rosa_wichuraiana Rw0G005780 Rw0G007540 Rw0G007550 Rw0G007560 Rw0G009390 Rw1G016900 Rw1G016930 Rw1G016940 Rw1G016970 Rw1G017860 Rw1G017890 Rw1G017900 Rw1G017910 Rw1G017920 Rw1G017960 Rw1G017970 Rw1G017980 Rw1G018150 Rw4G000830 Rw5G009290 Rw5G018950 Rw5G024850 Rw5G024870 Rw5G040460 Rw6G022290 Rw7G041020 Rw7G041200 Rw7G041360 Rw7G041450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AccB1I GGYRCC 2 cut(s) 261, 1604
AccII CGCG 2 cut(s) 306, 2308
AccIII TCCGGA 2 cut(s) 254, 2092
AciI CCGC 9 cut(s) 193, 304, 440, 942, 1007, 2306, 2308, 2409, 2538
AclWI GGATC 8 cut(s) 335, 640, 930, 2409, 2762, 2951, 3037, 3050
AcsI RAATTY 7 cut(s) 698, 725, 1056, 1657, 1757, 1793, 2311
AcuI CTGAAG 1 cut(s) 3056
AfaI GTAC 4 cut(s) 139, 1646, 2485, 3417
AfeI AGCGCT 1 cut(s) 1083
AfiI CCNNNNNNNGG 5 cut(s) 354, 2777, 3077, 3318, 3370
AflIII ACRYGT 1 cut(s) 3128
AhdI GACNNNNNGTC 1 cut(s) 247
AhlI ACTAGT 3 cut(s) 38, 47, 2233
AjiI CACGTC 1 cut(s) 919
AjnI CCWGG 7 cut(s) 973, 2037, 2246, 2697, 2775, 2815, 2830
AloI GAACNNNNNNTCC 2 cut(s) 2811, 2843
Alw21I GWGCWC 3 cut(s) 1024, 2267, 2546
Alw26I GTCTC 4 cut(s) 1864, 1975, 2457, 2814
Alw44I GTGCAC 1 cut(s) 2542
AlwI GGATC 8 cut(s) 335, 640, 930, 2409, 2762, 2951, 3037, 3050
AlwNI CAGNNNCTG 1 cut(s) 2782
Aor13HI TCCGGA 2 cut(s) 254, 2092
Aor51HI AGCGCT 1 cut(s) 1083
AoxI GGCC 3 cut(s) 2455, 2833, 3290
ApaLI GTGCAC 1 cut(s) 2542
ApeKI GCWGC 5 cut(s) 1735, 2182, 2389, 3482, 3495
ApoI RAATTY 7 cut(s) 698, 725, 1056, 1657, 1757, 1793, 2311
ArsI GACNNNNNNTTYG 2 cut(s) 355, 387
AseI ATTAAT 1 cut(s) 3018
Asp700I GAANNNNTTC 6 cut(s) 592, 1257, 1518, 1758, 2520, 2918
AspLEI GCGC 2 cut(s) 555, 1084
AspS9I GGNCC 7 cut(s) 931, 1111, 2601, 2859, 3050, 3290, 3298
AsuC2I CCSGG 1 cut(s) 3072
AsuHPI GGTGA 8 cut(s) 89, 436, 1076, 1280, 3086, 3146, 3154, 3473
AvaII GGWCC 6 cut(s) 931, 1111, 2601, 2859, 3050, 3298
BaeGI GKGCMC 2 cut(s) 501, 2546
BamHI GGATCC 1 cut(s) 3042
BanI GGYRCC 2 cut(s) 261, 1604
BanII GRGCYC 1 cut(s) 1024
BauI CACGAG 1 cut(s) 500
BbsI GAAGAC 3 cut(s) 243, 612, 2911
Bbv12I GWGCWC 3 cut(s) 1024, 2267, 2546
BbvI GCAGC 5 cut(s) 1747, 2169, 2376, 3494, 3507
BceAI ACGGC 1 cut(s) 1889
BciT130I CCWGG 7 cut(s) 975, 2039, 2248, 2699, 2777, 2817, 2832
BciVI GTATCC 2 cut(s) 1976, 2878
BclI TGATCA 2 cut(s) 282, 1315
BcnI CCSGG 1 cut(s) 3072
BcoDI GTCTC 4 cut(s) 1864, 1975, 2457, 2814
BcuI ACTAGT 3 cut(s) 38, 47, 2233
BfmI CTRYAG 1 cut(s) 1764
BfoI RGCGCY 1 cut(s) 1085
BfuI GTATCC 2 cut(s) 1976, 2878
BglII AGATCT 1 cut(s) 460
BisI GCNGC 7 cut(s) 441, 942, 1736, 2183, 2390, 3483, 3496
BlsI GCNGC 7 cut(s) 442, 943, 1737, 2184, 2391, 3484, 3497
BmcAI AGTACT 1 cut(s) 3417
Bme1390I CCNGG 8 cut(s) 975, 2039, 2248, 2699, 2777, 2817, 2832, 3072
Bme18I GGWCC 6 cut(s) 931, 1111, 2601, 2859, 3050, 3298
BmeRI GACNNNNNGTC 1 cut(s) 247
BmgBI CACGTC 1 cut(s) 919
BmgT120I GGNCC 7 cut(s) 931, 1111, 2601, 2859, 3050, 3290, 3298
BmiI GGNNCC 8 cut(s) 263, 1539, 1606, 2414, 2602, 2646, 2860, 3044
BmrFI CCNGG 8 cut(s) 975, 2039, 2248, 2699, 2777, 2817, 2832, 3072
BmsI GCATC 9 cut(s) 657, 725, 868, 945, 1564, 1573, 1689, 2076, 3201
BpiI GAAGAC 3 cut(s) 243, 612, 2911
BpmI CTGGAG 4 cut(s) 1703, 2060, 2838, 3285
Bpu10I CCTNAGC 1 cut(s) 1038
BpuEI CTTGAG 3 cut(s) 85, 2251, 2344
BpuMI CCSGG 1 cut(s) 3072
BsaBI GATNNNNATC 2 cut(s) 339, 2955
BsaI GGTCTC 1 cut(s) 2457
BsaJI CCNNGG 9 cut(s) 164, 1533, 2247, 2306, 2706, 2776, 3046, 3237, 3301
BsaWI WCCGGW 3 cut(s) 254, 2092, 2431
BsaXI ACNNNNNCTCC 6 cut(s) 13, 43, 1698, 1728, 2811, 2841
Bsc4I CCNNNNNNNGG 5 cut(s) 354, 2777, 3077, 3318, 3370
Bse1I ACTGG 3 cut(s) 2458, 2836, 3110
Bse8I GATNNNNATC 2 cut(s) 339, 2955
BseAI TCCGGA 2 cut(s) 254, 2092
BseBI CCWGG 7 cut(s) 975, 2039, 2248, 2699, 2777, 2817, 2832
BseDI CCNNGG 9 cut(s) 164, 1533, 2247, 2306, 2706, 2776, 3046, 3237, 3301
BseJI GATNNNNATC 2 cut(s) 339, 2955
BseLI CCNNNNNNNGG 5 cut(s) 354, 2777, 3077, 3318, 3370
BseMII CTCAG 4 cut(s) 941, 1738, 1753, 2419
BseNI ACTGG 3 cut(s) 2458, 2836, 3110
BseRI GAGGAG 1 cut(s) 1706
BseSI GKGCMC 2 cut(s) 501, 2546
BseXI GCAGC 5 cut(s) 1747, 2169, 2376, 3494, 3507
BsgI GTGCAG 2 cut(s) 1754, 2768
Bsh1236I CGCG 2 cut(s) 306, 2308
BshFI GGCC 3 cut(s) 2457, 2835, 3292
BshNI GGYRCC 2 cut(s) 261, 1604
BsiHKAI GWGCWC 3 cut(s) 1024, 2267, 2546
BsiSI CCGG 4 cut(s) 255, 2093, 2432, 3071
BsiWI CGTACG 1 cut(s) 137
BslFI GGGAC 2 cut(s) 183, 2793
BslI CCNNNNNNNGG 5 cut(s) 354, 2777, 3077, 3318, 3370
BsmAI GTCTC 4 cut(s) 1864, 1975, 2457, 2814
BsmBI CGTCTC 2 cut(s) 1864, 2814
BsmFI GGGAC 2 cut(s) 183, 2793
BsmI GAATGC 2 cut(s) 1346, 1422
BsnI GGCC 3 cut(s) 2457, 2835, 3292
Bso31I GGTCTC 1 cut(s) 2457
Bsp1286I GDGCHC 4 cut(s) 501, 1024, 2267, 2546
Bsp13I TCCGGA 2 cut(s) 254, 2092
Bsp1407I TGTACA 1 cut(s) 2483
Bsp19I CCATGG 1 cut(s) 3046
BspACI CCGC 9 cut(s) 193, 304, 440, 942, 1007, 2306, 2308, 2409, 2538
BspANI GGCC 3 cut(s) 2457, 2835, 3292
BspCNI CTCAG 4 cut(s) 942, 1737, 1752, 2418
BspEI TCCGGA 2 cut(s) 254, 2092
BspFNI CGCG 2 cut(s) 306, 2308
BspHI TCATGA 3 cut(s) 1318, 1933, 2020
BspLI GGNNCC 8 cut(s) 263, 1539, 1606, 2414, 2602, 2646, 2860, 3044
BspMAI CTGCAG 1 cut(s) 1768
BspPI GGATC 8 cut(s) 335, 640, 930, 2409, 2762, 2951, 3037, 3050
BspT107I GGYRCC 2 cut(s) 261, 1604
BspTNI GGTCTC 1 cut(s) 2457
BsrGI TGTACA 1 cut(s) 2483
BsrI ACTGG 3 cut(s) 2458, 2836, 3110
BssECI CCNNGG 9 cut(s) 164, 1533, 2247, 2306, 2706, 2776, 3046, 3237, 3301
BssSI CACGAG 1 cut(s) 500
BssT1I CCWWGG 4 cut(s) 164, 1533, 2706, 3046
Bst2BI CACGAG 1 cut(s) 500
Bst2UI CCWGG 7 cut(s) 975, 2039, 2248, 2699, 2777, 2817, 2832
Bst4CI ACNGT 6 cut(s) 1163, 1400, 2262, 2878, 2987, 3010
Bst6I CTCTTC 1 cut(s) 1504
BstAUI TGTACA 1 cut(s) 2483
BstC8I GCNNGC 4 cut(s) 276, 785, 1098, 1778
BstDSI CCRYGG 3 cut(s) 2306, 3046, 3237
BstEII GGTNACC 2 cut(s) 1450, 3461
BstFNI CGCG 2 cut(s) 306, 2308
BstH2I RGCGCY 1 cut(s) 1085
BstHHI GCGC 2 cut(s) 555, 1084
BstMAI GTCTC 4 cut(s) 1864, 1975, 2457, 2814
BstMWI GCNNNNNNNGC 4 cut(s) 464, 2841, 2850, 3501
BstNI CCWGG 7 cut(s) 975, 2039, 2248, 2699, 2777, 2817, 2832
BstNSI RCATGY 3 cut(s) 1100, 1639, 3132
BstPI GGTNACC 2 cut(s) 1450, 3461
BstSCI CCNGG 8 cut(s) 973, 2037, 2246, 2697, 2775, 2815, 2830, 3070
BstSFI CTRYAG 1 cut(s) 1764
BstSLI GKGCMC 2 cut(s) 501, 2546
BstUI CGCG 2 cut(s) 306, 2308
BstV1I GCAGC 5 cut(s) 1747, 2169, 2376, 3494, 3507
BstV2I GAAGAC 3 cut(s) 243, 612, 2911
BstX2I RGATCY 4 cut(s) 460, 2401, 2767, 3042
BstXI CCANNNNNNTGG 3 cut(s) 584, 779, 1121
BstYI RGATCY 4 cut(s) 460, 2401, 2767, 3042
BsuI GTATCC 2 cut(s) 1976, 2878
BsuRI GGCC 3 cut(s) 2457, 2835, 3292
BtgI CCRYGG 3 cut(s) 2306, 3046, 3237
BtgZI GCGATG 2 cut(s) 293, 445
BtrI CACGTC 1 cut(s) 919
BtsI GCAGTG 1 cut(s) 1024
BtsIMutI CAGTG 5 cut(s) 1024, 1159, 1455, 1747, 3103
Cac8I GCNNGC 4 cut(s) 276, 785, 1098, 1778
CaiI CAGNNNCTG 1 cut(s) 2782
CciI TCATGA 3 cut(s) 1318, 1933, 2020
CfoI GCGC 2 cut(s) 555, 1084
Cfr13I GGNCC 7 cut(s) 931, 1111, 2601, 2859, 3050, 3290, 3298
Cfr42I CCGCGG 1 cut(s) 2309
CpoI CGGWCCG 1 cut(s) 3298
CseI GACGC 2 cut(s) 237, 1755
Csp6I GTAC 4 cut(s) 138, 1645, 2484, 3416
CspI CGGWCCG 1 cut(s) 3298
CviQI GTAC 4 cut(s) 138, 1645, 2484, 3416
DriI GACNNNNNGTC 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 1504
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 1504
Ecl136II GAGCTC 1 cut(s) 1022
Eco130I CCWWGG 4 cut(s) 164, 1533, 2706, 3046
Eco24I GRGCYC 1 cut(s) 1024
Eco31I GGTCTC 1 cut(s) 2457
Eco32I GATATC 2 cut(s) 178, 3376
Eco47I GGWCC 6 cut(s) 931, 1111, 2601, 2859, 3050, 3298
Eco47III AGCGCT 1 cut(s) 1083
Eco53kI GAGCTC 1 cut(s) 1022
Eco57I CTGAAG 1 cut(s) 3056
Eco91I GGTNACC 2 cut(s) 1450, 3461
EcoICRI GAGCTC 1 cut(s) 1022
EcoO109I RGGNCCY 1 cut(s) 2859
EcoO65I GGTNACC 2 cut(s) 1450, 3461
EcoRI GAATTC 1 cut(s) 1056
EcoRII CCWGG 7 cut(s) 973, 2037, 2246, 2697, 2775, 2815, 2830
EcoRV GATATC 2 cut(s) 178, 3376
EcoT14I CCWWGG 4 cut(s) 164, 1533, 2706, 3046
EcoT22I ATGCAT 3 cut(s) 1488, 1690, 2753
EcoT38I GRGCYC 1 cut(s) 1024
ErhI CCWWGG 4 cut(s) 164, 1533, 2706, 3046
Esp3I CGTCTC 2 cut(s) 1864, 2814
FalI AAGNNNNNCTT 2 cut(s) 701, 733
FaqI GGGAC 2 cut(s) 183, 2793
FauI CCCGC 4 cut(s) 311, 1000, 2402, 2531
FauNDI CATATG 1 cut(s) 2753
FbaI TGATCA 2 cut(s) 282, 1315
Fnu4HI GCNGC 7 cut(s) 441, 942, 1736, 2183, 2390, 3483, 3496
FriOI GRGCYC 1 cut(s) 1024
Fsp4HI GCNGC 7 cut(s) 441, 942, 1736, 2183, 2390, 3483, 3496
GlaI GCGC 2 cut(s) 554, 1083
GluI GCNGC 7 cut(s) 441, 942, 1736, 2183, 2390, 3483, 3496
GsuI CTGGAG 4 cut(s) 1703, 2060, 2838, 3285
HaeII RGCGCY 1 cut(s) 1085
HaeIII GGCC 3 cut(s) 2457, 2835, 3292
HapII CCGG 4 cut(s) 255, 2093, 2432, 3071
HgaI GACGC 2 cut(s) 237, 1755
HhaI GCGC 2 cut(s) 555, 1084
Hin6I GCGC 2 cut(s) 553, 1082
HinP1I GCGC 2 cut(s) 553, 1082
HincII GTYRAC 1 cut(s) 3276
HindII GTYRAC 1 cut(s) 3276
HpaII CCGG 4 cut(s) 255, 2093, 2432, 3071
HphI GGTGA 8 cut(s) 89, 436, 1076, 1280, 3086, 3146, 3154, 3473
Hpy166II GTNNAC 5 cut(s) 931, 1745, 2544, 3205, 3276
Hpy8I GTNNAC 5 cut(s) 931, 1745, 2544, 3205, 3276
Hpy99I CGWCG 4 cut(s) 158, 497, 1551, 3298
HpyCH4III ACNGT 6 cut(s) 1163, 1400, 2262, 2878, 2987, 3010
HpyCH4IV ACGT 3 cut(s) 140, 918, 1549
HpyF10VI GCNNNNNNNGC 4 cut(s) 464, 2841, 2850, 3501
HpySE526I ACGT 3 cut(s) 140, 918, 1549
HspAI GCGC 2 cut(s) 553, 1082
Kpn2I TCCGGA 2 cut(s) 254, 2092
Ksp22I TGATCA 2 cut(s) 282, 1315
KspI CCGCGG 1 cut(s) 2309
LmnI GCTCC 2 cut(s) 1019, 1537
Lsp1109I GCAGC 5 cut(s) 1747, 2169, 2376, 3494, 3507
LweI GCATC 9 cut(s) 657, 725, 868, 945, 1564, 1573, 1689, 2076, 3201
MaeII ACGT 3 cut(s) 140, 918, 1549
MfeI CAATTG 2 cut(s) 1614, 2479
MflI RGATCY 4 cut(s) 460, 2401, 2767, 3042
MhlI GDGCHC 4 cut(s) 501, 1024, 2267, 2546
MlyI GAGTC 6 cut(s) 512, 1295, 1707, 1977, 2043, 2695
MmeI TCCRAC 9 cut(s) 68, 738, 1766, 1809, 2189, 2628, 2964, 3023, 3368
Mph1103I ATGCAT 3 cut(s) 1488, 1690, 2753
MroI TCCGGA 2 cut(s) 254, 2092
MroXI GAANNNNTTC 6 cut(s) 592, 1257, 1518, 1758, 2520, 2918
MseI TTAA 7 cut(s) 656, 810, 1185, 1791, 1916, 3018, 3152
MslI CAYNNNNRTG 6 cut(s) 1361, 1572, 1640, 1932, 2720, 2790
MspA1I CMGCKG 3 cut(s) 638, 2308, 2409
MspI CCGG 4 cut(s) 255, 2093, 2432, 3071
MspR9I CCNGG 8 cut(s) 975, 2039, 2248, 2699, 2777, 2817, 2832, 3072
MunI CAATTG 2 cut(s) 1614, 2479
Mva1269I GAATGC 2 cut(s) 1346, 1422
MvaI CCWGG 7 cut(s) 975, 2039, 2248, 2699, 2777, 2817, 2832
MvnI CGCG 2 cut(s) 306, 2308
MwoI GCNNNNNNNGC 4 cut(s) 464, 2841, 2850, 3501
NciI CCSGG 1 cut(s) 3072
NcoI CCATGG 1 cut(s) 3046
NdeI CATATG 1 cut(s) 2753
NlaIV GGNNCC 8 cut(s) 263, 1539, 1606, 2414, 2602, 2646, 2860, 3044
NmuCI GTSAC 8 cut(s) 210, 835, 1400, 1499, 2236, 3101, 3142, 3461
NsiI ATGCAT 3 cut(s) 1488, 1690, 2753
NspI RCATGY 3 cut(s) 1100, 1639, 3132
PaeI GCATGC 1 cut(s) 1100
PagI TCATGA 3 cut(s) 1318, 1933, 2020
PciI ACATGT 1 cut(s) 3128
PcsI WCGNNNNNNNCGW 2 cut(s) 142, 1552
PctI GAATGC 2 cut(s) 1346, 1422
PdmI GAANNNNTTC 6 cut(s) 592, 1257, 1518, 1758, 2520, 2918
Pfl23II CGTACG 1 cut(s) 137
PflFI GACNNNGTC 3 cut(s) 1551, 3339, 3467
PfoI TCCNGGA 3 cut(s) 973, 2037, 2815
PkrI GCNGC 7 cut(s) 442, 943, 1737, 2184, 2391, 3484, 3497
PleI GAGTC 6 cut(s) 511, 1295, 1707, 1977, 2042, 2694
PpsI GAGTC 6 cut(s) 511, 1295, 1707, 1977, 2042, 2694
PpuMI RGGWCCY 1 cut(s) 2859
PscI ACATGT 1 cut(s) 3128
PshBI ATTAAT 1 cut(s) 3018
PsiI TTATAA 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 1024
Psp5II RGGWCCY 1 cut(s) 2859
Psp6I CCWGG 7 cut(s) 973, 2037, 2246, 2697, 2775, 2815, 2830
PspEI GGTNACC 2 cut(s) 1450, 3461
PspGI CCWGG 7 cut(s) 973, 2037, 2246, 2697, 2775, 2815, 2830
PspLI CGTACG 1 cut(s) 137
PspN4I GGNNCC 8 cut(s) 263, 1539, 1606, 2414, 2602, 2646, 2860, 3044
PspPI GGNCC 7 cut(s) 931, 1111, 2601, 2859, 3050, 3290, 3298
PspPPI RGGWCCY 1 cut(s) 2859
PstI CTGCAG 1 cut(s) 1768
PstNI CAGNNNCTG 1 cut(s) 2782
PsuI RGATCY 4 cut(s) 460, 2401, 2767, 3042
PsyI GACNNNGTC 3 cut(s) 1551, 3339, 3467
PvuII CAGCTG 1 cut(s) 638
RsaI GTAC 4 cut(s) 139, 1646, 2485, 3417
RsaNI GTAC 4 cut(s) 138, 1645, 2484, 3416
RseI CAYNNNNRTG 6 cut(s) 1361, 1572, 1640, 1932, 2720, 2790
Rsr2I CGGWCCG 1 cut(s) 3298
RsrII CGGWCCG 1 cut(s) 3298
SacI GAGCTC 1 cut(s) 1024
SacII CCGCGG 1 cut(s) 2309
SaqAI TTAA 7 cut(s) 656, 810, 1185, 1791, 1916, 3018, 3152
SatI GCNGC 7 cut(s) 441, 942, 1736, 2183, 2390, 3483, 3496
Sau96I GGNCC 7 cut(s) 931, 1111, 2601, 2859, 3050, 3290, 3298
SbfI CCTGCAGG 1 cut(s) 1768
ScaI AGTACT 1 cut(s) 3417
SchI GAGTC 6 cut(s) 512, 1295, 1707, 1977, 2043, 2695
ScrFI CCNGG 8 cut(s) 975, 2039, 2248, 2699, 2777, 2817, 2832, 3072
SdaI CCTGCAGG 1 cut(s) 1768
SduI GDGCHC 4 cut(s) 501, 1024, 2267, 2546
SfaNI GCATC 9 cut(s) 657, 725, 868, 945, 1564, 1573, 1689, 2076, 3201
SfcI CTRYAG 1 cut(s) 1764
Sfr303I CCGCGG 1 cut(s) 2309
SgrBI CCGCGG 1 cut(s) 2309
SinI GGWCC 6 cut(s) 931, 1111, 2601, 2859, 3050, 3298
SmiMI CAYNNNNRTG 6 cut(s) 1361, 1572, 1640, 1932, 2720, 2790
SmlI CTYRAG 3 cut(s) 64, 2266, 2323
SmoI CTYRAG 3 cut(s) 64, 2266, 2323
SpeI ACTAGT 3 cut(s) 38, 47, 2233
SphI GCATGC 1 cut(s) 1100
Sse8387I CCTGCAGG 1 cut(s) 1768
SsiI CCGC 9 cut(s) 193, 304, 440, 942, 1007, 2306, 2308, 2409, 2538
SspI AATATT 4 cut(s) 850, 1587, 1600, 3358
SstI GAGCTC 1 cut(s) 1024
StyD4I CCNGG 8 cut(s) 973, 2037, 2246, 2697, 2775, 2815, 2830, 3070
StyI CCWWGG 4 cut(s) 164, 1533, 2706, 3046
TaaI ACNGT 6 cut(s) 1163, 1400, 2262, 2878, 2987, 3010
TaiI ACGT 3 cut(s) 143, 921, 1552
TaqII GACCGA 3 cut(s) 2914, 3261, 3315
TatI WGTACW 2 cut(s) 2483, 3415
TauI GCSGC 2 cut(s) 443, 944
Tru1I TTAA 7 cut(s) 656, 810, 1185, 1791, 1916, 3018, 3152
Tru9I TTAA 7 cut(s) 656, 810, 1185, 1791, 1916, 3018, 3152
TscAI CASTG 5 cut(s) 1031, 1166, 1462, 1747, 3110
TseFI GTSAC 8 cut(s) 210, 835, 1400, 1499, 2236, 3101, 3142, 3461
TseI GCWGC 5 cut(s) 1735, 2182, 2389, 3482, 3495
Tsp45I GTSAC 8 cut(s) 210, 835, 1400, 1499, 2236, 3101, 3142, 3461
TspGWI ACGGA 5 cut(s) 617, 1815, 3254, 3311, 3342
TspRI CASTG 5 cut(s) 1031, 1166, 1462, 1747, 3110
Tth111I GACNNNGTC 3 cut(s) 1551, 3339, 3467
VneI GTGCAC 1 cut(s) 2542
VpaK11BI GGWCC 6 cut(s) 931, 1111, 2601, 2859, 3050, 3298
VspI ATTAAT 1 cut(s) 3018
XapI RAATTY 7 cut(s) 698, 725, 1056, 1657, 1757, 1793, 2311
XbaI TCTAGA 2 cut(s) 1525, 1975
XceI RCATGY 3 cut(s) 1100, 1639, 3132
XcmI CCANNNNNNNNNTGG 1 cut(s) 2035
XmnI GAANNNNTTC 6 cut(s) 592, 1257, 1518, 1758, 2520, 2918
ZrmI AGTACT 1 cut(s) 3417
Zsp2I ATGCAT 3 cut(s) 1488, 1690, 2753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.