MD04G1162000.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000113
Physical Location & Seq
Forward (+)
25323803 .. 25324945
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1162000.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGAAGTCTCGCAAAATTTCCCATTCAATGGCGCCCAGCCTCCCTGATGAAATCAAACTCGACATACTCTCGAGGCTGCCGGTTAAATCTTTAATGCGATTTCGGTGTGTGTGCAAACCATGGCTTCATGTAATCTCCAATCCTCAGTTCGTTGCGCAACACCAGAGAAACACAGTAGTCACAAGCACCAGCAAGAACAACTTCAAGCTCTTCGTTGCAAAAATGTCAGACGACCATCTCGATCTCCTTTCCATAGACTATAATGATGATGTTGTTTCAACCAGAGTGCTCAATTATCCGTCAGTAATTAAACCAGATATGGTTATCGATATTTTGGGTTCGTGCAACGGTTTGGTATGTCTAGAAATTGACTTTGGCGAATTCCCTTACAACATTATCTTATGGAACCCTTCCACCGGAGATACCAATGTGTTACCGGAACCTCCTTATGTTGACCGTAATAAGATATTTTACGGATTAGGGTACGATTCCTCCGCTGAAGATTACAAGTTAATATTGGGCAGTCGTGACAGTACAACTTCAACTGAAGAACCTTTCCGATCCATGATACATGTCTTTACAGTTAAATCGAATTCATGGAGGACTTGTCGAGGCCTGTATTATCTTGAGAAGAATGATCAACGGGGCTGCTTCTTAAATGGAGCTCTTCACTGGATCCAGCTTCAGAGAAGAAGTTCCAAGATTGTCTCTTTTGATTTAGCAGAAGAAAATTTTAAGAGCACACTGTCATTGCCCCAACGTGACGATAGAGATATATTCGTCGGAATCGGGACCAGCCAAGACTGTCTCTTTGTGTATACTTCAGCGCTCCAGGATAAAACTTTTTCGATATGGATTATGAAGGAATATGGAGTCAAGGAATCTTGGACAAGAGTCGCGAAAATTTCATCAACGAGATTAGCTAGACAGATTTTTGTTGACTTTTGTGATCTTACTGTGATGCCTGTATGCATTTTCGAGAGTGGTGAAGTTTTGCTGGATCATGAGGGAAGGATCTTGGTGATCTATAATCCCAGGCAAAAAACATACAGAATTATTATCGAGGCTGACGACGAAAACGACGTTGAAGCAGTTACTTATCCAGAGACTTTGGATTTACCTGGAGGTGGTGCATACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like FBA_1

Protein Analysis

381

Amino Acids

43.47

Weight (kDa)

5.6

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 13 - 50 9.3e-10 F-box domain
F-box-like PF12937 13 - 49 2.9e-09 F-box-like
FBA_3 PF08268 72 - 353 1.1e-22 F-box associated beta propeller domain
FBA_1 PF07734 75 - 358 3.1e-32 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41473 AT3G16210
fragaria_vesca FvH4_1g00300 FvH4_1g03000 FvH4_1g03001 FvH4_2g08290 FvH4_2g08290 FvH4_3g33320 FvH4_3g33531 FvH4_3g40660 FvH4_3g41160 FvH4_4g09850 FvH4_4g09850 FvH4_4g09850 FvH4_6g33740 FvH4_6g33751 FvH4_6g39180 FvH4_6g39910 FvH4_6g39910 FvH4_6g39910 FvH4_6g39930 FvH4_6g39930 FvH4_6g40000 FvH4_6g40001 FvH4_6g40002 FvH4_6g40010 FvH4_6g40030 FvH4_6g40070 FvH4_6g40080 FvH4_6g40090 FvH4_6g47950 FvH4_6g47950 FvH4_7g09840 FvH4_7g09840 FvH4_7g09840 FvH4_7g09840 FvH4_7g25410 FvH4_7g25772 FvH4_7g25790 FvH4_7g25790
malus_domestica MD00G1070000.v1.1 MD00G1070100.v1.1 MD02G1002000.v1.1 MD04G1162000.v1.1 MD09G1129200.v1.1 MD09G1144400.v1.1 MD09G1144500.v1.1 MD15G1145500.v1.1 MD17G1124300.v1.1
prunus_persica Prupe.1G567200_v2.0.a1 Prupe.3G191200_v2.0.a1 Prupe.3G191300_v2.0.a1 Prupe.3G191300_v2.0.a1 Prupe.3G191300_v2.0.a1 Prupe.7G269000_v2.0.a1 Prupe.7G269000_v2.0.a1 Prupe.7G269000_v2.0.a1 Prupe.7G269000_v2.0.a1 Prupe.7G269000_v2.0.a1 Prupe.7G269000_v2.0.a1
pyrus_communis pycom02g00080 pycom02g00090 pycom09g05450 pycom09g06390 pycom15g13040 pycom15g13060 pycom17g11570
rosa_chinensis RchiOBHm_Chr1g0328801 RchiOBHm_Chr1g0347091 RchiOBHm_Chr1g0347101 RchiOBHm_Chr1g0347131 RchiOBHm_Chr1g0347171 RchiOBHm_Chr1g0347211 RchiOBHm_Chr1g0347321 RchiOBHm_Chr1g0347341 RchiOBHm_Chr1g0347361 RchiOBHm_Chr2g0084671 RchiOBHm_Chr2g0153011 RchiOBHm_Chr2g0154441 RchiOBHm_Chr2g0154521 RchiOBHm_Chr2g0154531 RchiOBHm_Chr2g0154541 RchiOBHm_Chr2g0154551 RchiOBHm_Chr2g0154561 RchiOBHm_Chr2g0154571 RchiOBHm_Chr2g0154581 RchiOBHm_Chr2g0154591 RchiOBHm_Chr2g0154601 RchiOBHm_Chr2g0154611 RchiOBHm_Chr2g0154621 RchiOBHm_Chr2g0154641 RchiOBHm_Chr2g0154651 RchiOBHm_Chr2g0154661 RchiOBHm_Chr2g0154671 RchiOBHm_Chr2g0154681 RchiOBHm_Chr2g0154711 RchiOBHm_Chr2g0167131 RchiOBHm_Chr5g0060681 RchiOBHm_Chr5g0060691 RchiOBHm_Chr5g0060711 RchiOBHm_Chr5g0061011 RchiOBHm_Chr6g0275741
rosa_laevigata RLG00000013437 RLG00000015630 RLG00000020778 RLG00000020784 RLG00000020785 RLG00000020787 RLG00000020788 RLG00000020790 RLG00000020791 RLG00000020792 RLG00000020794 RLG00000020795 RLG00000020796 RLG00000020797 RLG00000021699 RLG00000028755 RLG00000035394
rosa_multiflora Rmu_co8119446.1_g000001 Rmu_co8175998.1_g000001 Rmu_co8210288.1_g000001 Rmu_co8317779.1_g000001 Rmu_co8324277.1_g000001 Rmu_co8343471.1_g000001 Rmu_co8346313.1_g000001 Rmu_co8407145.1_g000001 Rmu_co8411851.1_g000001 Rmu_co8437621.1_g000001 Rmu_sc0000218.1_g000006 Rmu_sc0000640.1_g000006 Rmu_sc0000864.1_g000001 Rmu_sc0000864.1_g000002 Rmu_sc0000864.1_g000004 Rmu_sc0000864.1_g000007 Rmu_sc0001004.1_g000008 Rmu_sc0001004.1_g000016 Rmu_sc0001004.1_g000017 Rmu_sc0001004.1_g000023 Rmu_sc0001004.1_g000026 Rmu_sc0001004.1_g000027 Rmu_sc0001004.1_g000033 Rmu_sc0001004.1_g000034 Rmu_sc0001004.1_g000035 Rmu_sc0001027.1_g000008 Rmu_sc0001027.1_g000011 Rmu_sc0001027.1_g000015 Rmu_sc0001027.1_g000019 Rmu_sc0001027.1_g000021 Rmu_sc0001027.1_g000022 Rmu_sc0001027.1_g000023 Rmu_sc0001027.1_g000026 Rmu_sc0001027.1_g000028 Rmu_sc0001027.1_g000029 Rmu_sc0002705.1_g000031 Rmu_sc0002705.1_g000033 Rmu_sc0002705.1_g000036 Rmu_sc0002705.1_g000037 Rmu_sc0003808.1_g000017 Rmu_sc0003808.1_g000018 Rmu_sc0004001.1_g000015 Rmu_sc0006475.1_g000019 Rmu_sc0008818.1_g000006 Rmu_sc0013419.1_g000015 Rmu_sc0015771.1_g000021 Rmu_sc0016102.1_g000001 Rmu_sc0016442.1_g000001 Rmu_sc0016843.1_g000001 Rmu_sc0016843.1_g000002 Rmu_sc0032116.1_g000001
rosa_roxburghii Rroxscaffold_1G00019640 Rroxscaffold_1G00020010 Rroxscaffold_1G00020070 Rroxscaffold_1G00020110 Rroxscaffold_2G00083690 Rroxscaffold_2G00094170 Rroxscaffold_2G00094180 Rroxscaffold_2G00094190 Rroxscaffold_2G00094200 Rroxscaffold_2G00094210 Rroxscaffold_2G00094220 Rroxscaffold_2G00094230 Rroxscaffold_2G00094240 Rroxscaffold_2G00094250 Rroxscaffold_2G00094260 Rroxscaffold_2G00094330 Rroxscaffold_2G00155920 Rroxscaffold_3G00250730 Rroxscaffold_4G00307870 Rroxscaffold_4G00307880 Rroxscaffold_4G00307900 Rroxscaffold_4G00307910 Rroxscaffold_4G00307970 Rroxscaffold_7G00192940
rosa_rugosa Rorug01G0185500 Rorug01G0185600 Rorug01G0185900 Rorug01G0186100 Rorug02G0085700 Rorug02G0444400 Rorug02G0444600 Rorug02G0444700 Rorug02G0444700 Rorug02G0444800 Rorug04G0120600 Rorug05G0332700 Rorug05G0332800 Rorug05G0332900 Rorug05G0333000 Rorug05G0336000 Rorug06G0095900
rosa_samantha Rh2AG003200 Rh2BG004100 Rh2BG606800 Rh2CG004200 Rh2DG003900 Rh2DG531100 Rh6BG210600 Rh6CG214300 Rh6DG203900
rosa_wichuraiana Rw1G007390 Rw1G017020 Rw1G017100 Rw2G000310 Rw2G041790 Rw2G041850 Rw2G041860 Rw2G041870 Rw2G041880 Rw2G041890 Rw2G041900 Rw2G041920 Rw2G041940 Rw2G049600 Rw4G015020 Rw5G037320 Rw5G037330 Rw5G037340 Rw6G018130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 156
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 28
AccI GTMKAC 1 cut(s) 818
AccII CGCG 1 cut(s) 899
AciI CCGC 1 cut(s) 495
AclWI GGATC 5 cut(s) 555, 670, 683, 1008, 1022
AcsI RAATTY 5 cut(s) 15, 380, 592, 730, 903
AcuI CTGAAG 4 cut(s) 519, 567, 668, 807
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 485, 535
AfeI AGCGCT 1 cut(s) 828
AfiI CCNNNNNNNGG 3 cut(s) 28, 416, 1127
AflIII ACRYGT 2 cut(s) 571, 1137
AgsI TTSAA 5 cut(s) 27, 205, 279, 543, 1088
AjnI CCWGG 3 cut(s) 831, 1034, 1120
AjuI GAANNNNNNNTTGG 4 cut(s) 131, 163, 357, 389
AluBI AGCT 4 cut(s) 208, 665, 682, 923
AluI AGCT 4 cut(s) 208, 665, 682, 923
Alw21I GWGCWC 3 cut(s) 291, 667, 743
Alw26I GTCTC 4 cut(s) 12, 712, 812, 1100
AlwI GGATC 5 cut(s) 555, 670, 683, 1008, 1022
Ama87I CYCGRG 1 cut(s) 70
Aor51HI AGCGCT 1 cut(s) 828
AoxI GGCC 1 cut(s) 613
ApeKI GCWGC 2 cut(s) 76, 648
ApoI RAATTY 5 cut(s) 15, 380, 592, 730, 903
ArsI GACNNNNNNTTYG 2 cut(s) 794, 826
Asp700I GAANNNNTTC 2 cut(s) 200, 694
AspLEI GCGC 3 cut(s) 34, 157, 829
AspS9I GGNCC 1 cut(s) 792
AsuHPI GGTGA 2 cut(s) 998, 1033
AvaI CYCGRG 1 cut(s) 70
AvaII GGWCC 1 cut(s) 792
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BamHI GGATCC 1 cut(s) 675
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 667
Bbv12I GWGCWC 3 cut(s) 291, 667, 743
BbvI GCAGC 2 cut(s) 63, 635
BccI CCATC 1 cut(s) 243
BciT130I CCWGG 3 cut(s) 833, 1036, 1122
BclI TGATCA 1 cut(s) 637
BcoDI GTCTC 4 cut(s) 12, 712, 812, 1100
BfaI CTAG 2 cut(s) 362, 924
BfoI RGCGCY 2 cut(s) 35, 830
BisI GCNGC 2 cut(s) 77, 649
BlsI GCNGC 2 cut(s) 78, 650
Bme1390I CCNGG 3 cut(s) 833, 1036, 1122
Bme18I GGWCC 1 cut(s) 792
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 792
BmiI GGNNCC 5 cut(s) 33, 407, 441, 677, 793
BmrFI CCNGG 3 cut(s) 833, 1036, 1122
BmsI GCATC 1 cut(s) 951
BoxI GACNNNNGTC 1 cut(s) 893
BpmI CTGGAG 2 cut(s) 815, 1143
BpuEI CTTGAG 1 cut(s) 647
Bsa29I ATCGAT 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 119, 1034
BsaWI WCCGGW 2 cut(s) 416, 436
BsaXI ACNNNNNCTCC 4 cut(s) 476, 506, 592, 622
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 416, 1127
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 677
Bse3DI GCAATG 1 cut(s) 749
BseBI CCWGG 3 cut(s) 833, 1036, 1122
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 2 cut(s) 119, 1034
BseLI CCNNNNNNNGG 3 cut(s) 28, 416, 1127
BseMI GCAATG 1 cut(s) 749
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 677
BseXI GCAGC 2 cut(s) 63, 635
BseYI CCCAGC 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 899
BshFI GGCC 1 cut(s) 615
BshNI GGYRCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 327
BsiHKAI GWGCWC 3 cut(s) 291, 667, 743
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 3 cut(s) 80, 417, 437
BslFI GGGAC 1 cut(s) 805
BslI CCNNNNNNNGG 3 cut(s) 28, 416, 1127
BsmAI GTCTC 4 cut(s) 12, 712, 812, 1100
BsmFI GGGAC 1 cut(s) 805
BsnI GGCC 1 cut(s) 615
BsoBI CYCGRG 1 cut(s) 70
Bsp1286I GDGCHC 3 cut(s) 291, 667, 743
Bsp143I GATC 8 cut(s) 241, 560, 637, 675, 949, 1000, 1014, 1023
Bsp19I CCATGG 1 cut(s) 119
Bsp68I TCGCGA 1 cut(s) 899
BspACI CCGC 1 cut(s) 495
BspANI GGCC 1 cut(s) 615
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 1 cut(s) 327
BspFNI CGCG 1 cut(s) 899
BspHI TCATGA 1 cut(s) 1003
BspLI GGNNCC 5 cut(s) 33, 407, 441, 677, 793
BspPI GGATC 5 cut(s) 555, 670, 683, 1008, 1022
BspQI GCTCTTC 2 cut(s) 215, 672
BspT107I GGYRCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 749
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 1 cut(s) 677
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 2 cut(s) 119, 1034
BssMI GATC 8 cut(s) 241, 560, 637, 675, 949, 1000, 1014, 1023
BssNAI GTATAC 1 cut(s) 819
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 119
Bst1107I GTATAC 1 cut(s) 819
Bst2UI CCWGG 3 cut(s) 833, 1036, 1122
Bst4CI ACNGT 8 cut(s) 175, 350, 458, 533, 583, 747, 806, 958
Bst6I CTCTTC 2 cut(s) 215, 672
BstACI GRCGYC 1 cut(s) 32
BstDEI CTNAG 1 cut(s) 144
BstDSI CCRYGG 1 cut(s) 119
BstFNI CGCG 1 cut(s) 899
BstH2I RGCGCY 2 cut(s) 35, 830
BstHHI GCGC 3 cut(s) 34, 157, 829
BstKTI GATC 8 cut(s) 244, 563, 640, 678, 952, 1003, 1017, 1026
BstMAI GTCTC 4 cut(s) 12, 712, 812, 1100
BstMBI GATC 8 cut(s) 241, 560, 637, 675, 949, 1000, 1014, 1023
BstNI CCWGG 3 cut(s) 833, 1036, 1122
BstNSI RCATGY 2 cut(s) 575, 1141
BstPAI GACNNNNGTC 1 cut(s) 893
BstSCI CCNGG 3 cut(s) 831, 1034, 1120
BstUI CGCG 1 cut(s) 899
BstV1I GCAGC 2 cut(s) 63, 635
BstX2I RGATCY 2 cut(s) 675, 1014
BstYI RGATCY 2 cut(s) 675, 1014
BstZ17I GTATAC 1 cut(s) 819
Bsu15I ATCGAT 1 cut(s) 327
BsuRI GGCC 1 cut(s) 615
BsuTUI ATCGAT 1 cut(s) 327
BtgI CCRYGG 1 cut(s) 119
BtsIMutI CAGTG 2 cut(s) 670, 743
BtuMI TCGCGA 1 cut(s) 899
CciI TCATGA 1 cut(s) 1003
CfoI GCGC 3 cut(s) 34, 157, 829
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 792
ClaI ATCGAT 1 cut(s) 327
Csp6I GTAC 2 cut(s) 484, 534
CviAII CATG 7 cut(s) 120, 128, 565, 572, 597, 1004, 1138
CviQI GTAC 2 cut(s) 484, 534
DdeI CTNAG 1 cut(s) 144
DinI GGCGCC 1 cut(s) 33
DpnI GATC 8 cut(s) 243, 562, 639, 677, 951, 1002, 1016, 1025
DpnII GATC 8 cut(s) 241, 560, 637, 675, 949, 1000, 1014, 1023
Eam1104I CTCTTC 2 cut(s) 215, 672
EarI CTCTTC 2 cut(s) 215, 672
Ecl136II GAGCTC 1 cut(s) 665
Eco130I CCWWGG 1 cut(s) 119
Eco147I AGGCCT 1 cut(s) 615
Eco24I GRGCYC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 792
Eco47III AGCGCT 1 cut(s) 828
Eco53kI GAGCTC 1 cut(s) 665
Eco57I CTGAAG 4 cut(s) 519, 567, 668, 807
Eco88I CYCGRG 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 665
EcoRI GAATTC 2 cut(s) 380, 592
EcoRII CCWGG 3 cut(s) 831, 1034, 1120
EcoT14I CCWWGG 1 cut(s) 119
EcoT22I ATGCAT 1 cut(s) 974
EcoT38I GRGCYC 1 cut(s) 667
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 7 cut(s) 123, 131, 568, 575, 600, 1007, 1141
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FaqI GGGAC 1 cut(s) 805
FatI CATG 7 cut(s) 119, 127, 564, 571, 596, 1003, 1137
FbaI TGATCA 1 cut(s) 637
FblI GTMKAC 1 cut(s) 818
Fnu4HI GCNGC 2 cut(s) 77, 649
FriOI GRGCYC 1 cut(s) 667
Fsp4HI GCNGC 2 cut(s) 77, 649
FspBI CTAG 2 cut(s) 362, 924
FspI TGCGCA 1 cut(s) 156
GlaI GCGC 3 cut(s) 33, 156, 828
GluI GCNGC 2 cut(s) 77, 649
GsaI CCCAGC 1 cut(s) 39
GsuI CTGGAG 2 cut(s) 815, 1143
HaeII RGCGCY 2 cut(s) 35, 830
HaeIII GGCC 1 cut(s) 615
HapII CCGG 3 cut(s) 80, 417, 437
HhaI GCGC 3 cut(s) 34, 157, 829
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 7 cut(s) 123, 131, 568, 575, 600, 1007, 1141
Hin6I GCGC 3 cut(s) 32, 155, 827
HinP1I GCGC 3 cut(s) 32, 155, 827
HincII GTYRAC 2 cut(s) 454, 940
HindII GTYRAC 2 cut(s) 454, 940
HinfI GANTC 5 cut(s) 488, 786, 873, 881, 894
HpaII CCGG 3 cut(s) 80, 417, 437
HphI GGTGA 2 cut(s) 998, 1033
Hpy166II GTNNAC 3 cut(s) 454, 819, 940
Hpy188I TCNGA 4 cut(s) 229, 560, 687, 785
Hpy8I GTNNAC 3 cut(s) 454, 819, 940
Hpy99I CGWCG 3 cut(s) 785, 1076, 1085
HpyAV CCTTC 3 cut(s) 420, 856, 1005
HpyCH4III ACNGT 8 cut(s) 175, 350, 458, 533, 583, 747, 806, 958
HpyCH4IV ACGT 2 cut(s) 760, 1083
HpyCH4V TGCA 5 cut(s) 114, 218, 345, 972, 1133
HpyF3I CTNAG 1 cut(s) 144
HpySE526I ACGT 2 cut(s) 760, 1083
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 7 cut(s) 123, 131, 568, 575, 600, 1007, 1141
HspAI GCGC 3 cut(s) 32, 155, 827
KasI GGCGCC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 8 cut(s) 241, 560, 637, 675, 949, 1000, 1014, 1023
LguI GCTCTTC 2 cut(s) 215, 672
LmnI GCTCC 2 cut(s) 662, 834
Lsp1109I GCAGC 2 cut(s) 63, 635
LweI GCATC 1 cut(s) 951
MaeI CTAG 2 cut(s) 362, 924
MaeII ACGT 2 cut(s) 760, 1083
MaeIII GTNAC 5 cut(s) 178, 432, 527, 761, 1093
MalI GATC 8 cut(s) 243, 562, 639, 677, 951, 1002, 1016, 1025
MboI GATC 8 cut(s) 241, 560, 637, 675, 949, 1000, 1014, 1023
MboII GAAGA 7 cut(s) 202, 512, 560, 643, 659, 702, 737
MflI RGATCY 2 cut(s) 675, 1014
MhlI GDGCHC 3 cut(s) 291, 667, 743
MluCI AATT 9 cut(s) 15, 292, 306, 366, 380, 592, 730, 903, 1053
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 2 cut(s) 882, 903
MmeI TCCRAC 1 cut(s) 763
Mph1103I ATGCAT 1 cut(s) 974
MroXI GAANNNNTTC 2 cut(s) 200, 694
MseI TTAA 7 cut(s) 84, 92, 309, 512, 585, 656, 735
MslI CAYNNNNRTG 1 cut(s) 1138
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 3 cut(s) 80, 417, 437
MspR9I CCNGG 3 cut(s) 833, 1036, 1122
MvaI CCWGG 3 cut(s) 833, 1036, 1122
MvnI CGCG 1 cut(s) 899
NarI GGCGCC 1 cut(s) 32
NcoI CCATGG 1 cut(s) 119
NdeII GATC 8 cut(s) 241, 560, 637, 675, 949, 1000, 1014, 1023
NlaIII CATG 7 cut(s) 123, 131, 568, 575, 600, 1007, 1141
NlaIV GGNNCC 5 cut(s) 33, 407, 441, 677, 793
NmuCI GTSAC 3 cut(s) 178, 527, 761
NruI TCGCGA 1 cut(s) 899
NsbI TGCGCA 1 cut(s) 156
NsiI ATGCAT 1 cut(s) 974
NspI RCATGY 2 cut(s) 575, 1141
PaeR7I CTCGAG 1 cut(s) 70
PagI TCATGA 1 cut(s) 1003
PceI AGGCCT 1 cut(s) 615
PciI ACATGT 2 cut(s) 571, 1137
PciSI GCTCTTC 2 cut(s) 215, 672
PcsI WCGNNNNNNNCGW 4 cut(s) 237, 1068, 1077, 1080
PdmI GAANNNNTTC 2 cut(s) 200, 694
PfeI GAWTC 3 cut(s) 488, 786, 881
PflMI CCANNNNNTGG 1 cut(s) 28
PfoI TCCNGGA 1 cut(s) 831
PkrI GCNGC 2 cut(s) 78, 650
PleI GAGTC 2 cut(s) 881, 902
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 2 cut(s) 881, 902
PscI ACATGT 2 cut(s) 571, 1137
PshAI GACNNNNGTC 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 667
Psp6I CCWGG 3 cut(s) 831, 1034, 1120
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 3 cut(s) 831, 1034, 1120
PspN4I GGNNCC 5 cut(s) 33, 407, 441, 677, 793
PspPI GGNCC 1 cut(s) 792
PsuI RGATCY 2 cut(s) 675, 1014
RruI TCGCGA 1 cut(s) 899
RsaI GTAC 2 cut(s) 485, 535
RsaNI GTAC 2 cut(s) 484, 534
RseI CAYNNNNRTG 1 cut(s) 1138
SacI GAGCTC 1 cut(s) 667
SapI GCTCTTC 2 cut(s) 215, 672
SaqAI TTAA 7 cut(s) 84, 92, 309, 512, 585, 656, 735
SatI GCNGC 2 cut(s) 77, 649
Sau3AI GATC 8 cut(s) 241, 560, 637, 675, 949, 1000, 1014, 1023
Sau96I GGNCC 1 cut(s) 792
SchI GAGTC 2 cut(s) 882, 903
ScrFI CCNGG 3 cut(s) 833, 1036, 1122
SduI GDGCHC 3 cut(s) 291, 667, 743
SfaNI GCATC 1 cut(s) 951
SfoI GGCGCC 1 cut(s) 33
Sfr274I CTCGAG 1 cut(s) 70
SinI GGWCC 1 cut(s) 792
SlaI CTCGAG 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 1138
SmlI CTYRAG 2 cut(s) 70, 626
SmoI CTYRAG 2 cut(s) 70, 626
Sse9I AATT 9 cut(s) 15, 292, 306, 366, 380, 592, 730, 903, 1053
SseBI AGGCCT 1 cut(s) 615
SsiI CCGC 1 cut(s) 495
SspDI GGCGCC 1 cut(s) 31
SspI AATATT 1 cut(s) 516
SspMI CTAG 2 cut(s) 362, 924
SstI GAGCTC 1 cut(s) 667
StuI AGGCCT 1 cut(s) 615
StyD4I CCNGG 3 cut(s) 831, 1034, 1120
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 8 cut(s) 175, 350, 458, 533, 583, 747, 806, 958
TaiI ACGT 2 cut(s) 763, 1086
TaqI TCGA 9 cut(s) 60, 71, 240, 327, 590, 610, 848, 978, 1062
TasI AATT 9 cut(s) 15, 292, 306, 366, 380, 592, 730, 903, 1053
TatI WGTACW 1 cut(s) 533
TfiI GAWTC 3 cut(s) 488, 786, 881
Tru1I TTAA 7 cut(s) 84, 92, 309, 512, 585, 656, 735
Tru9I TTAA 7 cut(s) 84, 92, 309, 512, 585, 656, 735
TscAI CASTG 2 cut(s) 677, 750
TseFI GTSAC 3 cut(s) 178, 527, 761
TseI GCWGC 2 cut(s) 76, 648
Tsp45I GTSAC 3 cut(s) 178, 527, 761
TspDTI ATGAA 6 cut(s) 17, 63, 116, 585, 875, 897
TspGWI ACGGA 2 cut(s) 288, 489
TspRI CASTG 2 cut(s) 677, 750
Van91I CCANNNNNTGG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 792
XapI RAATTY 5 cut(s) 15, 380, 592, 730, 903
XbaI TCTAGA 1 cut(s) 361
XceI RCATGY 2 cut(s) 575, 1141
XhoI CTCGAG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 818
XmnI GAANNNNTTC 2 cut(s) 200, 694
XspI CTAG 2 cut(s) 362, 924
Zsp2I ATGCAT 1 cut(s) 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.