Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000121

Physical Location & Seq

Genomic Coordinates Reverse (-)

5105509 .. 5107460

Gene Structure 1952 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1063600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGCATCCTTGCCATATATTGACGTATAAACAATACGATTTGGTAAATTGGGAGATAAAGTCATGCATCATAAGACAGACTGATCATCTACAGAGAGAAATGAAGAGCAATATGGTTGTTATTTTAGGGGTTTTTGTGGCAATGTTTGTGCAAAGTGTGGCAACACAGAAAGTGCATGTGGTGGGAGATAACATGGGTTGGACTATTCCAGGATCTGGGGCACAAGAATATGTAAATTGGGCTGCTACAAACAACTTCACGGTTGGTGATACACTCGTTTTCAACTTCATCACCGGTGTACACAACGTTGTCCAAGTAACGAAGGCATCATTCCACTCATGCAACGAAGAAGAACGAATTGGCTCATCCCTCACAACTGGCCCAACAAACATCACACTCACCTCCGCAGGCGACCACTACTACATCTGCAATTTTAGCAATCACTGCCAGGTAGGCATGAAGCTAGCTGTCACCGTATCCAGTGCTTCCCCAGAATCGCCACTGCAAGGAACTGTGTATCTAGTCTTTGGTGTAGTTTTTAGCATACTTGTTTTCTCTCCCTTCCTAGAGATTTTCCGGCCACGAGGAAAAGTCAAGTCCTTCGACTCCAACCATGGGAAGACAAAGTCAACAGAGACCCCGGGATCACCTTTACCGACTTATCTATGTCGTTACTTTCCACTGGCTGAGATTAAAGCCGCCACCCAAAACTTTAACGAGACTTTGGTTATTGGTGTTGGCGGGTTTGGCAATGTGTACAAAGGGTGTATTGATGGTGGTGTCACTCCTGTTGCTATCAAACGGTTGAAACGGGAATCATCACAGGGGGCCCGTGAGTTCAAGACGGAAATCGAGCTGCTTTCCCAACTCCGCCACCGCCATTTGGTTTCACTCATTGGTTACTGCTCCGATAATGGCGAGATGATCTTGGTTTATGATTACATGGCACGAGGCACTCTCGGTGACCATCTCTATCGCACTGGTAACCTTAAACCTCTTCCTTGGGAACAACGACTCCAAATTTGTATTGGCGCAGCACGAGGTTTGCACTACCTTCACAGTGGTGCCAATGGCTCCATTATTCACCGTGATGTTAAGAGCACAAATATTTTATTGGATGAGAAAATGGTCGCCAAGGTTTCAGATTTCGGGTTGTCAAAAATGGGCACGATTAACACGTCCAAAACGCACATCAGCACGGTGGTGAAAGGTAGTTTCGGGTATCTAGACCCAGAATACTTTCGACGTCAACAGCTAACTGAGAAGTCTGATGTGTACTCATTTGGAGTGGTTTTGTGGGAGGTATTGTGTGCGAGGCCAGCTTTGCTGCATACGGTAGAGAGTAGGCAAATCAACCTCGCTGAGTGGGCCAAGAGTTGTCATGGAGACGAGACACTTGACCAAATCATTGACCCAAATGTGAAGGGTCAAATTGAAGTCGAGTGCTTGAACAAGTTCATTGAGATTGCTATAAGTTGCTTACATGATAAAGGAATCGAACGACCGTCGATGAATGATGTTGTGAAGGGCCTTGAGTTTGCACTGCAGCTTCATCAAAAGAGCATTGGAAGTAAGGGTGACAATACCAGTAGCACCAAGCAAAGTTGTGCTACCAATGAATCCATACAACATATATCTGTGACAATCTTCTCTGAGATCAATGAATCAACTGGACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

560

Amino Acids

61.97

Weight (kDa)

7.29

Isoelectric Point (pI)

43.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu_bind_like	PF02298	67 - 153	2.3e-24	Plastocyanin-like domain
Pkinase	PF00069	242 - 509	4e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	243 - 510	1.5e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000121)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46570 FvH4_6g46580 FvH4_6g46610 FvH4_6g46620 FvH4_6g46640 FvH4_6g46680
malus_domestica	MD03G1063600.v1.1 MD03G1063700.v1.1 MD09G1069300.v1.1 MD09G1069400.v1.1 MD11G1067200.v1.1 MD11G1070300.v1.1 MD11G1153300.v1.1 MD17G1060300.v1.1 MD17G1061900.v1.1 MD17G1062100.v1.1 MD17G1062300.v1.1 MD17G1062400.v1.1 MD17G1063000.v1.1 MD17G1063200.v1.1 MD17G1063600.v1.1
prunus_persica	Prupe.3G246200_v2.0.a1 Prupe.3G251000_v2.0.a1 Prupe.3G251100_v2.0.a1 Prupe.3G251200_v2.0.a1 Prupe.3G251600_v2.0.a1 Prupe.3G251700_v2.0.a1 Prupe.3G251800_v2.0.a1 Prupe.3G251900_v2.0.a1
pyrus_communis	pycom03g05070 pycom03g05100 pycom12433g00070 pycom17g06000 pycom17g06170 pycom17g06180 pycom17g06200 pycom17g06210 pycom17g06280 pycom17g06300 pycom17g06310 pycom17g06320 pycom17g06330 pycom17g06350 pycom17g06370 pycom17g06380 pycom17g06400
rosa_chinensis	RchiOBHm_Chr1g0321851 RchiOBHm_Chr1g0321911 RchiOBHm_Chr1g0323951 RchiOBHm_Chr1g0361941 RchiOBHm_Chr2g0164711 RchiOBHm_Chr2g0165301 RchiOBHm_Chr2g0165311 RchiOBHm_Chr2g0165321 RchiOBHm_Chr2g0165351 RchiOBHm_Chr2g0165431 RchiOBHm_Chr2g0165461 RchiOBHm_Chr2g0165491 RchiOBHm_Chr2g0165511 RchiOBHm_Chr2g0165521 RchiOBHm_Chr2g0165531 RchiOBHm_Chr3g0481631 RchiOBHm_Chr3g0481801 RchiOBHm_Chr3g0481811 RchiOBHm_Chr3g0481821 RchiOBHm_Chr3g0481831 RchiOBHm_Chr4g0400291 RchiOBHm_Chr4g0400371 RchiOBHm_Chr4g0400391 RchiOBHm_Chr6g0274711 RchiOBHm_Chr6g0274721 RchiOBHm_Chr7g0211011
rosa_laevigata	RLG00000002260 RLG00000002262 RLG00000002263 RLG00000003027 RLG00000003028 RLG00000009196 RLG00000009197 RLG00000012573 RLG00000020400 RLG00000021523 RLG00000021524 RLG00000021525 RLG00000021527 RLG00000021528 RLG00000021529 RLG00000021530 RLG00000021532 RLG00000021533 RLG00000021534 RLG00000021535 RLG00000021537 RLG00000023384 RLG00000027681 RLG00000027682 RLG00000027685 RLG00000030240 RLG00000030401
rosa_multiflora	Rmu_co7998078.1_g000001 Rmu_co8009032.1_g000001 Rmu_co8326071.1_g000001 Rmu_co8333709.1_g000001 Rmu_co8362007.1_g000001 Rmu_co8369261.1_g000001 Rmu_co8391219.1_g000001 Rmu_co8456951.1_g000001 Rmu_co8461523.1_g000001 Rmu_sc0000427.1_g000009 Rmu_sc0000427.1_g000032 Rmu_sc0000427.1_g000033 Rmu_sc0000521.1_g000005 Rmu_sc0000521.1_g000008 Rmu_sc0000521.1_g000009 Rmu_sc0000655.1_g000016 Rmu_sc0000655.1_g000017 Rmu_sc0000655.1_g000018 Rmu_sc0000675.1_g000003 Rmu_sc0000795.1_g000018 Rmu_sc0000795.1_g000083 Rmu_sc0001306.1_g000042 Rmu_sc0001306.1_g000043 Rmu_sc0001873.1_g000001 Rmu_sc0001873.1_g000010 Rmu_sc0002529.1_g000002 Rmu_sc0002529.1_g000004 Rmu_sc0003410.1_g000004 Rmu_sc0003909.1_g000010 Rmu_sc0004097.1_g000001 Rmu_sc0005425.1_g000003 Rmu_sc0005425.1_g000004 Rmu_sc0005425.1_g000010 Rmu_sc0005425.1_g000012 Rmu_sc0005425.1_g000017 Rmu_sc0006223.1_g000014 Rmu_sc0006399.1_g000007 Rmu_sc0010324.1_g000001 Rmu_sc0010409.1_g000005 Rmu_sc0013532.1_g000005 Rmu_sc0016601.1_g000002 Rmu_sc0016601.1_g000003 Rmu_sc0016601.1_g000006 Rmu_sc0017773.1_g000001 Rmu_sc0017773.1_g000002 Rmu_sc0019514.1_g000002 Rmu_sc0021575.1_g000001 Rmu_sc0023720.1_g000002 Rmu_sc0030898.1_g000001 Rmu_sc0033982.1_g000001 Rmu_sc0034651.1_g000001 Rmu_sc0038482.1_g000001 Rmu_sc0038644.1_g000001 Rmu_sc0038644.1_g000003 Rmu_sc0039243.1_g000001 Rmu_sc0042715.1_g000001 Rmu_ssc0000124.1_g000003
rosa_roxburghii	Rroxscaffold_2G00085740 Rroxscaffold_2G00085760 Rroxscaffold_2G00085780 Rroxscaffold_2G00085800 Rroxscaffold_2G00085830 Rroxscaffold_2G00085850 Rroxscaffold_2G00085860 Rroxscaffold_4G00294610 Rroxscaffold_4G00326070 Rroxscaffold_4G00326080 Rroxscaffold_4G00327420 Rroxscaffold_4G00327430 Rroxscaffold_4G00327460 Rroxscaffold_4G00327470 Rroxscaffold_5G00345350 Rroxscaffold_6G00399890 Rroxscaffold_6G00400250 Rroxscaffold_7G00194000 Rroxscaffold_7G00194020 Rroxscaffold_7G00194050
rosa_rugosa	Rorug01G0034300 Rorug01G0034500 Rorug01G0040700 Rorug01G0064300 Rorug02G0513700 Rorug02G0513800 Rorug02G0513900 Rorug02G0514000 Rorug02G0514000 Rorug02G0514100 Rorug02G0514300 Rorug02G0514400 Rorug02G0514700 Rorug02G0515000 Rorug02G0515100 Rorug02G0515300 Rorug02G0515500 Rorug02G0515600 Rorug02G0515800 Rorug02G0515900 Rorug02G0516000 Rorug03G0193200 Rorug04G0024600 Rorug06G0083100 Rorug06G0085200 Rorug07G0188600
rosa_samantha	Rh1CG234000 Rh2DG603700 Rh2DG603800 Rh2DG603900 Rh6BG203000
rosa_wichuraiana	Rw0G010290 Rw1G004240 Rw1G005000 Rw1G026960 Rw2G048310 Rw2G048320 Rw2G048330 Rw2G048350 Rw2G048360 Rw2G048380 Rw2G048430 Rw2G048460 Rw2G048500 Rw2G048510 Rw2G048520 Rw2G048530 Rw3G021810 Rw4G008270 Rw6G017320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1249
AccB1I	GGYRCC	1 cut(s)	1064
AccB7I	CCANNNNNTGG	1 cut(s)	215
AciI	CCGC	5 cut(s)	405, 699, 741, 871, 877
AclI	AACGTT	1 cut(s)	306
AclWI	GGATC	2 cut(s)	220, 652
AcoI	YGGCCR	1 cut(s)	578
AcsI	RAATTY	1 cut(s)	1020
AcyI	GRCGYC	1 cut(s)	1246
AfaI	GTAC	3 cut(s)	300, 758, 1277
AfiI	CCNNNNNNNGG	4 cut(s)	215, 506, 615, 883
AflIII	ACRYGT	1 cut(s)	1176
AgeI	ACCGGT	1 cut(s)	293
AgsI	TTSAA	5 cut(s)	283, 808, 841, 1436, 1450
AjiI	CACGTC	1 cut(s)	1179
AjnI	CCWGG	2 cut(s)	208, 447
AjuI	GAANNNNNNNTTGG	2 cut(s)	342, 374
AleI	CACNNNNGTG	2 cut(s)	1062, 1202
AloI	GAACNNNNNNTCC	2 cut(s)	999, 1031
AluBI	AGCT	6 cut(s)	463, 467, 856, 1255, 1322, 1549
AluI	AGCT	6 cut(s)	463, 467, 856, 1255, 1322, 1549
Alw21I	GWGCWC	1 cut(s)	1103
Alw26I	GTCTC	4 cut(s)	629, 713, 1380, 1385
AlwI	GGATC	2 cut(s)	220, 652
AlwNI	CAGNNNCTG	1 cut(s)	215
Ama87I	CYCGRG	1 cut(s)	640
AoxI	GGCC	6 cut(s)	379, 578, 828, 1316, 1368, 1528
ApaI	GGGCCC	1 cut(s)	832
ApeKI	GCWGC	5 cut(s)	242, 856, 1034, 1327, 1546
ApoI	RAATTY	1 cut(s)	1020
ArsI	GACNNNNNNTTYG	2 cut(s)	1005, 1037
AsiGI	ACCGGT	1 cut(s)	293
Asp700I	GAANNNNTTC	2 cut(s)	1239, 1454
AspLEI	GCGC	1 cut(s)	1034
AspS9I	GGNCC	5 cut(s)	380, 828, 829, 1368, 1528
AsuC2I	CCSGG	2 cut(s)	641, 642
AsuHPI	GGTGA	9 cut(s)	278, 283, 391, 463, 639, 974, 1076, 1216, 1589
AsuNHI	GCTAGC	1 cut(s)	463
AvaI	CYCGRG	1 cut(s)	640
BaeGI	GKGCMC	3 cut(s)	223, 832, 1169
BaeI	ACNNNNGTAYC	2 cut(s)	261, 294
BanI	GGYRCC	1 cut(s)	1064
BanII	GRGCYC	1 cut(s)	832
BauI	CACGAG	3 cut(s)	582, 948, 1038
BbsI	GAAGAC	1 cut(s)	626
Bbv12I	GWGCWC	1 cut(s)	1103
BbvI	GCAGC	5 cut(s)	229, 843, 1046, 1314, 1558
BccI	CCATC	2 cut(s)	767, 975
BciT130I	CCWGG	2 cut(s)	210, 449
BciVI	GTATCC	1 cut(s)	487
BclI	TGATCA	1 cut(s)	82
BcnI	CCSGG	2 cut(s)	641, 642
BcoDI	GTCTC	4 cut(s)	629, 713, 1380, 1385
BfaI	CTAG	4 cut(s)	464, 521, 566, 1226
BfmI	CTRYAG	2 cut(s)	89, 1544
BfuI	GTATCC	1 cut(s)	487
BglI	GCCNNNNNGGC	1 cut(s)	453
BisI	GCNGC	6 cut(s)	243, 699, 857, 1035, 1328, 1547
BlsI	GCNGC	6 cut(s)	244, 700, 858, 1036, 1329, 1548
Bme1390I	CCNGG	4 cut(s)	210, 449, 641, 642
BmeT110I	CYCGRG	1 cut(s)	640
BmgBI	CACGTC	1 cut(s)	1179
BmgT120I	GGNCC	5 cut(s)	380, 828, 829, 1368, 1528
BmiI	GGNNCC	4 cut(s)	829, 830, 1066, 1075
BmrFI	CCNGG	4 cut(s)	210, 449, 641, 642
BmsI	GCATC	3 cut(s)	13, 75, 335
BmtI	GCTAGC	1 cut(s)	467
BpiI	GAAGAC	1 cut(s)	626
BplI	GAGNNNNNCTC	2 cut(s)	942, 974
BpuEI	CTTGAG	1 cut(s)	1553
BpuMI	CCSGG	2 cut(s)	641, 642
BsaHI	GRCGYC	1 cut(s)	1246
BsaI	GGTCTC	1 cut(s)	629
BsaJI	CCNNGG	5 cut(s)	613, 639, 640, 1001, 1134
BsaWI	WCCGGW	1 cut(s)	293
BsaXI	ACNNNNNCTCC	6 cut(s)	44, 74, 999, 1029, 1292, 1322
Bsc4I	CCNNNNNNNGG	4 cut(s)	215, 506, 615, 883
Bse118I	RCCGGY	1 cut(s)	293
Bse1I	ACTGG	6 cut(s)	382, 480, 687, 985, 1587, 1675
Bse3DI	GCAATG	2 cut(s)	147, 757
BseBI	CCWGG	2 cut(s)	210, 449
BseDI	CCNNGG	5 cut(s)	613, 639, 640, 1001, 1134
BseGI	GGATG	3 cut(s)	4, 365, 1123
BseLI	CCNNNNNNNGG	4 cut(s)	215, 506, 615, 883
BseMI	GCAATG	2 cut(s)	147, 757
BseMII	CTCAG	4 cut(s)	678, 1251, 1353, 1644
BseNI	ACTGG	6 cut(s)	382, 480, 687, 985, 1587, 1675
BseSI	GKGCMC	3 cut(s)	223, 832, 1169
BseXI	GCAGC	5 cut(s)	229, 843, 1046, 1314, 1558
Bsh1285I	CGRYCG	1 cut(s)	1505
BshFI	GGCC	6 cut(s)	381, 580, 830, 1318, 1370, 1530
BshNI	GGYRCC	1 cut(s)	1064
BshTI	ACCGGT	1 cut(s)	293
BsiEI	CGRYCG	1 cut(s)	1505
BsiHKAI	GWGCWC	1 cut(s)	1103
BsiHKCI	CYCGRG	1 cut(s)	640
BsiSI	CCGG	3 cut(s)	294, 577, 641
BslI	CCNNNNNNNGG	4 cut(s)	215, 506, 615, 883
BsmAI	GTCTC	4 cut(s)	629, 713, 1380, 1385
BsmBI	CGTCTC	1 cut(s)	1380
BsnI	GGCC	6 cut(s)	381, 580, 830, 1318, 1370, 1530
Bso31I	GGTCTC	1 cut(s)	629
BsoBI	CYCGRG	1 cut(s)	640
Bsp120I	GGGCCC	1 cut(s)	828
Bsp1286I	GDGCHC	4 cut(s)	223, 832, 1103, 1169
Bsp1407I	TGTACA	2 cut(s)	298, 756
Bsp143I	GATC	5 cut(s)	82, 212, 644, 924, 1656
Bsp19I	CCATGG	1 cut(s)	613
BspACI	CCGC	5 cut(s)	405, 699, 741, 871, 877
BspANI	GGCC	6 cut(s)	381, 580, 830, 1318, 1370, 1530
BspCNI	CTCAG	4 cut(s)	679, 1252, 1354, 1645
BspLI	GGNNCC	4 cut(s)	829, 830, 1066, 1075
BspMAI	CTGCAG	1 cut(s)	1548
BspOI	GCTAGC	1 cut(s)	467
BspPI	GGATC	2 cut(s)	220, 652
BspQI	GCTCTTC	1 cut(s)	98
BspT107I	GGYRCC	1 cut(s)	1064
BspTNI	GGTCTC	1 cut(s)	629
BsrDI	GCAATG	2 cut(s)	147, 757
BsrFI	RCCGGY	1 cut(s)	293
BsrGI	TGTACA	2 cut(s)	298, 756
BsrI	ACTGG	6 cut(s)	382, 480, 687, 985, 1587, 1675
BssAI	RCCGGY	1 cut(s)	293
BssECI	CCNNGG	5 cut(s)	613, 639, 640, 1001, 1134
BssMI	GATC	5 cut(s)	82, 212, 644, 924, 1656
BssNI	GRCGYC	1 cut(s)	1246
BssSI	CACGAG	3 cut(s)	582, 948, 1038
BssT1I	CCWWGG	3 cut(s)	613, 1001, 1134
Bst2BI	CACGAG	3 cut(s)	582, 948, 1038
Bst2UI	CCWGG	2 cut(s)	210, 449
Bst4CI	ACNGT	9 cut(s)	262, 475, 514, 804, 1061, 1088, 1201, 1336, 1506
Bst6I	CTCTTC	2 cut(s)	98, 1002
BstACI	GRCGYC	1 cut(s)	1246
BstAPI	GCANNNNNTGC	1 cut(s)	444
BstAUI	TGTACA	2 cut(s)	298, 756
BstC8I	GCNNGC	3 cut(s)	409, 465, 1320
BstDEI	CTNAG	4 cut(s)	687, 1260, 1362, 1653
BstDSI	CCRYGG	1 cut(s)	613
BstEII	GGTNACC	2 cut(s)	962, 983
BstF5I	GGATG	3 cut(s)	4, 365, 1123
BstHHI	GCGC	1 cut(s)	1034
BstKTI	GATC	5 cut(s)	85, 215, 647, 927, 1659
BstMAI	GTCTC	4 cut(s)	629, 713, 1380, 1385
BstMBI	GATC	5 cut(s)	82, 212, 644, 924, 1656
BstMCI	CGRYCG	1 cut(s)	1505
BstMWI	GCNNNNNNNGC	7 cut(s)	435, 444, 453, 747, 1319, 1324, 1367
BstNI	CCWGG	2 cut(s)	210, 449
BstNSI	RCATGY	1 cut(s)	179
BstPI	GGTNACC	2 cut(s)	962, 983
BstSCI	CCNGG	4 cut(s)	208, 447, 639, 640
BstSFI	CTRYAG	2 cut(s)	89, 1544
BstSLI	GKGCMC	3 cut(s)	223, 832, 1169
BstV1I	GCAGC	5 cut(s)	229, 843, 1046, 1314, 1558
BstV2I	GAAGAC	1 cut(s)	626
BstX2I	RGATCY	1 cut(s)	212
BstYI	RGATCY	1 cut(s)	212
BsuI	GTATCC	1 cut(s)	487
BsuRI	GGCC	6 cut(s)	381, 580, 830, 1318, 1370, 1530
BtgI	CCRYGG	1 cut(s)	613
BtrI	CACGTC	1 cut(s)	1179
BtsCI	GGATG	3 cut(s)	4, 365, 1123
BtsI	GCAGTG	3 cut(s)	442, 500, 1541
BtsIMutI	CAGTG	7 cut(s)	442, 487, 500, 680, 978, 1066, 1541
Cac8I	GCNNGC	3 cut(s)	409, 465, 1320
CaiI	CAGNNNCTG	1 cut(s)	215
CfoI	GCGC	1 cut(s)	1034
Cfr10I	RCCGGY	1 cut(s)	293
Cfr13I	GGNCC	5 cut(s)	380, 828, 829, 1368, 1528
Cfr9I	CCCGGG	1 cut(s)	640
Csp6I	GTAC	3 cut(s)	299, 757, 1276
CspAI	ACCGGT	1 cut(s)	293
CviAII	CATG	9 cut(s)	63, 176, 193, 339, 457, 614, 943, 1382, 1484
CviQI	GTAC	3 cut(s)	299, 757, 1276
DdeI	CTNAG	4 cut(s)	687, 1260, 1362, 1653
DpnI	GATC	5 cut(s)	84, 214, 646, 926, 1658
DpnII	GATC	5 cut(s)	82, 212, 644, 924, 1656
EaeI	YGGCCR	1 cut(s)	578
Eam1104I	CTCTTC	2 cut(s)	98, 1002
EarI	CTCTTC	2 cut(s)	98, 1002
EciI	GGCGGA	1 cut(s)	860
Eco130I	CCWWGG	3 cut(s)	613, 1001, 1134
Eco24I	GRGCYC	1 cut(s)	832
Eco31I	GGTCTC	1 cut(s)	629
Eco88I	CYCGRG	1 cut(s)	640
Eco91I	GGTNACC	2 cut(s)	962, 983
EcoO109I	RGGNCCY	2 cut(s)	828, 1528
EcoO65I	GGTNACC	2 cut(s)	962, 983
EcoRII	CCWGG	2 cut(s)	208, 447
EcoT14I	CCWWGG	3 cut(s)	613, 1001, 1134
EcoT22I	ATGCAT	2 cut(s)	6, 68
EcoT38I	GRGCYC	1 cut(s)	832
ErhI	CCWWGG	3 cut(s)	613, 1001, 1134
Esp3I	CGTCTC	1 cut(s)	1380
FaeI	CATG	9 cut(s)	66, 179, 196, 342, 460, 617, 946, 1385, 1487
FatI	CATG	9 cut(s)	62, 175, 192, 338, 456, 613, 942, 1381, 1483
FauI	CCCGC	1 cut(s)	734
FbaI	TGATCA	1 cut(s)	82
Fnu4HI	GCNGC	6 cut(s)	243, 699, 857, 1035, 1328, 1547
FokI	GGATG	2 cut(s)	352, 1130
FriOI	GRGCYC	1 cut(s)	832
Fsp4HI	GCNGC	6 cut(s)	243, 699, 857, 1035, 1328, 1547
FspBI	CTAG	4 cut(s)	464, 521, 566, 1226
GlaI	GCGC	1 cut(s)	1033
GluI	GCNGC	6 cut(s)	243, 699, 857, 1035, 1328, 1547
HaeIII	GGCC	6 cut(s)	381, 580, 830, 1318, 1370, 1530
HapII	CCGG	3 cut(s)	294, 577, 641
HhaI	GCGC	1 cut(s)	1034
Hin1I	GRCGYC	1 cut(s)	1246
Hin1II	CATG	9 cut(s)	66, 179, 196, 342, 460, 617, 946, 1385, 1487
Hin6I	GCGC	1 cut(s)	1032
HinP1I	GCGC	1 cut(s)	1032
HincII	GTYRAC	2 cut(s)	630, 1250
HindII	GTYRAC	2 cut(s)	630, 1250
HinfI	GANTC	7 cut(s)	494, 605, 815, 1014, 1494, 1619, 1664
HpaII	CCGG	3 cut(s)	294, 577, 641
HphI	GGTGA	9 cut(s)	278, 283, 391, 463, 639, 974, 1076, 1216, 1589
Hpy166II	GTNNAC	6 cut(s)	299, 301, 630, 757, 1250, 1276
Hpy188I	TCNGA	4 cut(s)	910, 1144, 1270, 1654
Hpy188III	TCNNGA	2 cut(s)	841, 1226
Hpy8I	GTNNAC	6 cut(s)	299, 301, 630, 757, 1250, 1276
Hpy99I	CGWCG	2 cut(s)	1248, 1510
HpyAV	CCTTC	6 cut(s)	316, 571, 610, 1064, 1417, 1519
HpyCH4III	ACNGT	9 cut(s)	262, 475, 514, 804, 1061, 1088, 1201, 1336, 1506
HpyCH4IV	ACGT	4 cut(s)	23, 306, 1178, 1246
HpyF10VI	GCNNNNNNNGC	7 cut(s)	435, 444, 453, 747, 1319, 1324, 1367
HpyF3I	CTNAG	4 cut(s)	687, 1260, 1362, 1653
HpySE526I	ACGT	4 cut(s)	23, 306, 1178, 1246
Hsp92I	GRCGYC	1 cut(s)	1246
Hsp92II	CATG	9 cut(s)	66, 179, 196, 342, 460, 617, 946, 1385, 1487
HspAI	GCGC	1 cut(s)	1032
Ksp22I	TGATCA	1 cut(s)	82
Kzo9I	GATC	5 cut(s)	82, 212, 644, 924, 1656
LguI	GCTCTTC	1 cut(s)	98
LmnI	GCTCC	2 cut(s)	911, 1079
Lsp1109I	GCAGC	5 cut(s)	229, 843, 1046, 1314, 1558
LweI	GCATC	3 cut(s)	13, 75, 335
MaeI	CTAG	4 cut(s)	464, 521, 566, 1226
MaeII	ACGT	4 cut(s)	23, 306, 1178, 1246
MaeIII	GTNAC	9 cut(s)	316, 469, 671, 781, 899, 962, 983, 1577, 1639
MalI	GATC	5 cut(s)	84, 214, 646, 926, 1658
MboI	GATC	5 cut(s)	82, 212, 644, 924, 1656
MboII	GAAGA	6 cut(s)	115, 359, 362, 631, 989, 1639
MflI	RGATCY	1 cut(s)	212
MhlI	GDGCHC	4 cut(s)	223, 832, 1103, 1169
MluCI	AATT	6 cut(s)	46, 235, 357, 430, 1020, 1431
MlyI	GAGTC	2 cut(s)	599, 1008
MmeI	TCCRAC	2 cut(s)	179, 633
MnlI	CCTC	9 cut(s)	380, 412, 578, 944, 1005, 1034, 1294, 1308, 1367
Mph1103I	ATGCAT	2 cut(s)	6, 68
MroXI	GAANNNNTTC	2 cut(s)	1239, 1454
MseI	TTAA	5 cut(s)	693, 714, 990, 1095, 1173
MslI	CAYNNNNRTG	3 cut(s)	1062, 1089, 1202
MspI	CCGG	3 cut(s)	294, 577, 641
MspR9I	CCNGG	4 cut(s)	210, 449, 641, 642
MvaI	CCWGG	2 cut(s)	210, 449
MwoI	GCNNNNNNNGC	7 cut(s)	435, 444, 453, 747, 1319, 1324, 1367
NciI	CCSGG	2 cut(s)	641, 642
NcoI	CCATGG	1 cut(s)	613
NdeII	GATC	5 cut(s)	82, 212, 644, 924, 1656
NheI	GCTAGC	1 cut(s)	463
NlaIII	CATG	9 cut(s)	66, 179, 196, 342, 460, 617, 946, 1385, 1487
NlaIV	GGNNCC	4 cut(s)	829, 830, 1066, 1075
NmuCI	GTSAC	5 cut(s)	469, 781, 962, 1577, 1639
NsiI	ATGCAT	2 cut(s)	6, 68
NspI	RCATGY	1 cut(s)	179
OliI	CACNNNNGTG	2 cut(s)	1062, 1202
PciSI	GCTCTTC	1 cut(s)	98
PcsI	WCGNNNNNNNCGW	1 cut(s)	1175
PdmI	GAANNNNTTC	2 cut(s)	1239, 1454
PfeI	GAWTC	5 cut(s)	494, 815, 1494, 1619, 1664
PflFI	GACNNNGTC	1 cut(s)	625
PflMI	CCANNNNNTGG	1 cut(s)	215
PfoI	TCCNGGA	1 cut(s)	208
PinAI	ACCGGT	1 cut(s)	293
PkrI	GCNGC	6 cut(s)	244, 700, 858, 1036, 1329, 1548
PleI	GAGTC	2 cut(s)	599, 1008
PpsI	GAGTC	2 cut(s)	599, 1008
Psp1406I	AACGTT	1 cut(s)	306
Psp6I	CCWGG	2 cut(s)	208, 447
PspEI	GGTNACC	2 cut(s)	962, 983
PspGI	CCWGG	2 cut(s)	208, 447
PspN4I	GGNNCC	4 cut(s)	829, 830, 1066, 1075
PspOMI	GGGCCC	1 cut(s)	828
PspPI	GGNCC	5 cut(s)	380, 828, 829, 1368, 1528
PstI	CTGCAG	1 cut(s)	1548
PstNI	CAGNNNCTG	1 cut(s)	215
PsuI	RGATCY	1 cut(s)	212
PsyI	GACNNNGTC	1 cut(s)	625
RsaI	GTAC	3 cut(s)	300, 758, 1277
RsaNI	GTAC	3 cut(s)	299, 757, 1276
RseI	CAYNNNNRTG	3 cut(s)	1062, 1089, 1202
SapI	GCTCTTC	1 cut(s)	98
SaqAI	TTAA	5 cut(s)	693, 714, 990, 1095, 1173
SatI	GCNGC	6 cut(s)	243, 699, 857, 1035, 1328, 1547
Sau3AI	GATC	5 cut(s)	82, 212, 644, 924, 1656
Sau96I	GGNCC	5 cut(s)	380, 828, 829, 1368, 1528
SchI	GAGTC	2 cut(s)	599, 1008
ScrFI	CCNGG	4 cut(s)	210, 449, 641, 642
SduI	GDGCHC	4 cut(s)	223, 832, 1103, 1169
SfaNI	GCATC	3 cut(s)	13, 75, 335
SfcI	CTRYAG	2 cut(s)	89, 1544
SgrAI	CRCCGGYG	1 cut(s)	293
SmaI	CCCGGG	1 cut(s)	642
SmiMI	CAYNNNNRTG	3 cut(s)	1062, 1089, 1202
SmlI	CTYRAG	1 cut(s)	1532
SmoI	CTYRAG	1 cut(s)	1532
Sse9I	AATT	6 cut(s)	46, 235, 357, 430, 1020, 1431
SsiI	CCGC	5 cut(s)	405, 699, 741, 871, 877
SspI	AATATT	1 cut(s)	1108
SspMI	CTAG	4 cut(s)	464, 521, 566, 1226
StyD4I	CCNGG	4 cut(s)	208, 447, 639, 640
StyI	CCWWGG	3 cut(s)	613, 1001, 1134
TaaI	ACNGT	9 cut(s)	262, 475, 514, 804, 1061, 1088, 1201, 1336, 1506
TaiI	ACGT	4 cut(s)	26, 309, 1181, 1249
TaqI	TCGA	6 cut(s)	603, 852, 1243, 1440, 1497, 1508
TasI	AATT	6 cut(s)	46, 235, 357, 430, 1020, 1431
TatI	WGTACW	3 cut(s)	298, 756, 1275
TauI	GCSGC	1 cut(s)	701
TfiI	GAWTC	5 cut(s)	494, 815, 1494, 1619, 1664
Tru1I	TTAA	5 cut(s)	693, 714, 990, 1095, 1173
Tru9I	TTAA	5 cut(s)	693, 714, 990, 1095, 1173
TscAI	CASTG	7 cut(s)	449, 487, 507, 687, 985, 1066, 1548
TseFI	GTSAC	5 cut(s)	469, 781, 962, 1577, 1639
TseI	GCWGC	5 cut(s)	242, 856, 1034, 1327, 1546
Tsp45I	GTSAC	5 cut(s)	469, 781, 962, 1577, 1639
TspDTI	ATGAA	8 cut(s)	116, 277, 473, 1447, 1526, 1541, 1632, 1677
TspGWI	ACGGA	1 cut(s)	860
TspMI	CCCGGG	1 cut(s)	640
TspRI	CASTG	7 cut(s)	449, 487, 507, 687, 985, 1066, 1548
Tth111I	GACNNNGTC	1 cut(s)	625
Van91I	CCANNNNNTGG	1 cut(s)	215
XapI	RAATTY	1 cut(s)	1020
XbaI	TCTAGA	1 cut(s)	1225
XceI	RCATGY	1 cut(s)	179
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1025
XmaI	CCCGGG	1 cut(s)	640
XmnI	GAANNNNTTC	2 cut(s)	1239, 1454
XspI	CTAG	4 cut(s)	464, 521, 566, 1226
ZraI	GACGTC	1 cut(s)	1247
Zsp2I	ATGCAT	2 cut(s)	6, 68

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.