Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000121

Physical Location & Seq

Genomic Coordinates Forward (+)

80461962 .. 80464482

Gene Structure 2521 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ53348

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGGAACCGATTCTCATTTCTCTCTACCTCTCCTTGTTTCTCCACATCCTCACTCTCCTAGAGGCCGGTCAGTCGCCACCTATATACACTCCGCCGGATGATATCACCCTCGACTGTGGCAATTTGGGGCAACACTCGAATAGCTATGACCCCAGGATTTGGAGTGGAGACATCAACTCAACATTTTCCCCCAAACAAGATGGTGACAGTAACGCCACATCCCAATTCAGAAAAGCACCTCCGTCGTTATCACAATCCGCCAGCCAAGTCCCTTACACCACTGCCCGCCTTTCTTACTCGGAATTCACTTACAGAATTCCTCTTTCTCCCGGGAAAAAGTTCATCCGCTTGCATTTCAACCCGGCTTCATACCCAGACTTCCACATCTCCAACTCCCTCTTCTCTGTCACAGCTAGCCACTCCATCCGCGGCTACATCCTCCTCAAAGACTTCAGAGCTTACATCACTGCCGACGATGCTTACAGGGAGATGCCGCTCATGAGAGAATTCTGCGTCAACATAGAGACACAACATAGTTTGAACATCACATTCAGTCCCAGCAAAGATGCATACGCTTTCATCAATGGGATTGAAATCGTGTCTATGCCGTCCAAAATTTACACCACTTCAGTTCTTTCCATCGGCAGTGATAGCCCTTATTCATTCGAAAGCAGAATAGCTCTGGACATGGTGTACCGAATCAACATAGGCGGAAAGCCAATCAACACTACTGAAGACACCGGAATGTACCGGAAATGGGACGCTGCCGACGACATGTATTTGGATGGTTTTAGTAAAAAATACAGCGTGCTAGCACAGAACAATACAGTTGAGCTCAATTTTGTCGAAACTCCAGAGTACTCTGCTCCAAAAGAAGTTTACAATACAGGTCGGTCCCTGTGGATGAACAACACTATCAACAAGAGTTACAATCTCACTTGGTCATTTCCTGTAGACTCTAGCTTCTCCTACCTGGTTAGATTGCATTTTTGTGAGTTTGAGTCTGACGTTACCAAGAGCGGAGAGAGGACATTTGTAATTTACTTAGCCAATCAAACTGCTGAACAAGGAGCAGACGTAATTCTATGGAGTGGTGGAAATGGGATACCAACACTCAGAGACTACACCTTGCTCATGTTGGGCCCTGGAAGCCAGAAAAAAGTTAATCTCTCTATTGCATTGCAAGCATCACGAGTAGATGGGTCAACCGAATACAAGGATGCAATGTTGAATGGACTCGAAATCTTCAAGCTAATTTACCCAAAAGGTGATCTCAAAACCAACCCAGACCCAACAATGACACCGCATGGTAAATCGTTTCCTGTGCTTGCCCTCGTTGCTGGTGTAGTTTCCAGCATACTTGTGTTCTCTGTCCTCGGATACTTTGTTTTCAGGAAAAGATGGAAAGTCAAGGACCCTGACTACAGGATAAGGAAGTTCACGACGACCCCTGGGTCATCATATCCGTGTCGCTACTTTTCACTGGCAGAGATCAAAGCCGCCACCAAAAACTTCAGTGATGCTTTTATCATTGGAGGTGGAGGTTTTGGTAATGTATATAAAGGATGCATTGATGGTGGTGCCACTCCAGTTGCAATCAAACGGCTGAAACCAGAATCATCCCAAGGTGTCCATGAGTTTGAGACTGAAATTGAGATGCTCTCCCAACTCCGTCATCGCCATTTGGTTCCACTCATTGGATGTTGTACTGATAAAGGAGAGATGATCTTAGTGTATGATTACATGGCACAAGGGACCCTCCGTGACCATCTCTACCACACTAATACTCCACCTCTTCTTTGGGAACAACGGCTTCAGATTTGTATTGGTGCAGCTAGAGGATTGCAGTACCTCCATAGTGGTGTGAAGGGCACCATCATTCACCGAGATGTGAAGAGCACAAACATCTTATTGGATGACAACTGGGTGGCCAAAGTTTCAGATTTTGGGCTGTCCAAAGGCACAACAAACATGGCCAAAACCCACATCAGCACGATTGTGAAAGGCAGTTTCGGGTATCTGGATCCAGAATACTACCGACGACAACATCTGACTGAGAAGTCAGACGTCTACTCATTTGGTGTAGTTCTGTGCGAAGTATTATGTGCAAGGCCAGCAGTGATACATACAGAAGAGCTGAGGCAAGCCAGCCTGGCTGAGTGGGCCAAGAGCTGTCATCAGAACGGAGAACTTGATCAAATCATTGATCCAAGTTTGAGAGGTAAGATTGCAGCCGGCTGCTTGAATATATTCTCTGATGTAGCCATCAGTTGCATGCATGATGATGGGACCGAACGGCCATCCATGAATGATGTTGTAAGAGGGCTTGAGCTTGCTTTGGACCTTCAACCGAGCGCAGAGGGGAACATCAACTGCACTGAAAGGACTGGTAAAACTGAAACAAACAGTAGTGAGCAAAGTTGTGCAAACAACAATTCCATTATATGCATATCTGGGACAATCTTTTCTGAGATCAACAACCCAAGTGGGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009741 GO:0009787 GO:0009788 GO:0009791 GO:0009856 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010118 GO:0010483 GO:0010646 GO:0010648 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030054 GO:0030308 GO:0030312 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043207 GO:0043412 GO:0043680 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044426 GO:0044462 GO:0044464 GO:0044703 GO:0044706 GO:0045926 GO:0046777 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051703 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

831

Amino Acids

92.5

Weight (kDa)

6.16

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	38 - 417	2.5e-27	Malectin-like domain
Malectin	PF11721	231 - 368	1.5e-07	Malectin domain
Pkinase	PF00069	510 - 773	3.1e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	511 - 775	3.4e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000121)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g46570 FvH4_6g46580 FvH4_6g46610 FvH4_6g46620 FvH4_6g46640 FvH4_6g46680
malus_domestica	MD03G1063600.v1.1 MD03G1063700.v1.1 MD09G1069300.v1.1 MD09G1069400.v1.1 MD11G1067200.v1.1 MD11G1070300.v1.1 MD11G1153300.v1.1 MD17G1060300.v1.1 MD17G1061900.v1.1 MD17G1062100.v1.1 MD17G1062300.v1.1 MD17G1062400.v1.1 MD17G1063000.v1.1 MD17G1063200.v1.1 MD17G1063600.v1.1
prunus_persica	Prupe.3G246200_v2.0.a1 Prupe.3G251000_v2.0.a1 Prupe.3G251100_v2.0.a1 Prupe.3G251200_v2.0.a1 Prupe.3G251600_v2.0.a1 Prupe.3G251700_v2.0.a1 Prupe.3G251800_v2.0.a1 Prupe.3G251900_v2.0.a1
pyrus_communis	pycom03g05070 pycom03g05100 pycom12433g00070 pycom17g06000 pycom17g06170 pycom17g06180 pycom17g06200 pycom17g06210 pycom17g06280 pycom17g06300 pycom17g06310 pycom17g06320 pycom17g06330 pycom17g06350 pycom17g06370 pycom17g06380 pycom17g06400
rosa_chinensis	RchiOBHm_Chr1g0321851 RchiOBHm_Chr1g0321911 RchiOBHm_Chr1g0323951 RchiOBHm_Chr1g0361941 RchiOBHm_Chr2g0164711 RchiOBHm_Chr2g0165301 RchiOBHm_Chr2g0165311 RchiOBHm_Chr2g0165321 RchiOBHm_Chr2g0165351 RchiOBHm_Chr2g0165431 RchiOBHm_Chr2g0165461 RchiOBHm_Chr2g0165491 RchiOBHm_Chr2g0165511 RchiOBHm_Chr2g0165521 RchiOBHm_Chr2g0165531 RchiOBHm_Chr3g0481631 RchiOBHm_Chr3g0481801 RchiOBHm_Chr3g0481811 RchiOBHm_Chr3g0481821 RchiOBHm_Chr3g0481831 RchiOBHm_Chr4g0400291 RchiOBHm_Chr4g0400371 RchiOBHm_Chr4g0400391 RchiOBHm_Chr6g0274711 RchiOBHm_Chr6g0274721 RchiOBHm_Chr7g0211011
rosa_laevigata	RLG00000002260 RLG00000002262 RLG00000002263 RLG00000003027 RLG00000003028 RLG00000009196 RLG00000009197 RLG00000012573 RLG00000020400 RLG00000021523 RLG00000021524 RLG00000021525 RLG00000021527 RLG00000021528 RLG00000021529 RLG00000021530 RLG00000021532 RLG00000021533 RLG00000021534 RLG00000021535 RLG00000021537 RLG00000023384 RLG00000027681 RLG00000027682 RLG00000027685 RLG00000030240 RLG00000030401
rosa_multiflora	Rmu_co7998078.1_g000001 Rmu_co8009032.1_g000001 Rmu_co8326071.1_g000001 Rmu_co8333709.1_g000001 Rmu_co8362007.1_g000001 Rmu_co8369261.1_g000001 Rmu_co8391219.1_g000001 Rmu_co8456951.1_g000001 Rmu_co8461523.1_g000001 Rmu_sc0000427.1_g000009 Rmu_sc0000427.1_g000032 Rmu_sc0000427.1_g000033 Rmu_sc0000521.1_g000005 Rmu_sc0000521.1_g000008 Rmu_sc0000521.1_g000009 Rmu_sc0000655.1_g000016 Rmu_sc0000655.1_g000017 Rmu_sc0000655.1_g000018 Rmu_sc0000675.1_g000003 Rmu_sc0000795.1_g000018 Rmu_sc0000795.1_g000083 Rmu_sc0001306.1_g000042 Rmu_sc0001306.1_g000043 Rmu_sc0001873.1_g000001 Rmu_sc0001873.1_g000010 Rmu_sc0002529.1_g000002 Rmu_sc0002529.1_g000004 Rmu_sc0003410.1_g000004 Rmu_sc0003909.1_g000010 Rmu_sc0004097.1_g000001 Rmu_sc0005425.1_g000003 Rmu_sc0005425.1_g000004 Rmu_sc0005425.1_g000010 Rmu_sc0005425.1_g000012 Rmu_sc0005425.1_g000017 Rmu_sc0006223.1_g000014 Rmu_sc0006399.1_g000007 Rmu_sc0010324.1_g000001 Rmu_sc0010409.1_g000005 Rmu_sc0013532.1_g000005 Rmu_sc0016601.1_g000002 Rmu_sc0016601.1_g000003 Rmu_sc0016601.1_g000006 Rmu_sc0017773.1_g000001 Rmu_sc0017773.1_g000002 Rmu_sc0019514.1_g000002 Rmu_sc0021575.1_g000001 Rmu_sc0023720.1_g000002 Rmu_sc0030898.1_g000001 Rmu_sc0033982.1_g000001 Rmu_sc0034651.1_g000001 Rmu_sc0038482.1_g000001 Rmu_sc0038644.1_g000001 Rmu_sc0038644.1_g000003 Rmu_sc0039243.1_g000001 Rmu_sc0042715.1_g000001 Rmu_ssc0000124.1_g000003
rosa_roxburghii	Rroxscaffold_2G00085740 Rroxscaffold_2G00085760 Rroxscaffold_2G00085780 Rroxscaffold_2G00085800 Rroxscaffold_2G00085830 Rroxscaffold_2G00085850 Rroxscaffold_2G00085860 Rroxscaffold_4G00294610 Rroxscaffold_4G00326070 Rroxscaffold_4G00326080 Rroxscaffold_4G00327420 Rroxscaffold_4G00327430 Rroxscaffold_4G00327460 Rroxscaffold_4G00327470 Rroxscaffold_5G00345350 Rroxscaffold_6G00399890 Rroxscaffold_6G00400250 Rroxscaffold_7G00194000 Rroxscaffold_7G00194020 Rroxscaffold_7G00194050
rosa_rugosa	Rorug01G0034300 Rorug01G0034500 Rorug01G0040700 Rorug01G0064300 Rorug02G0513700 Rorug02G0513800 Rorug02G0513900 Rorug02G0514000 Rorug02G0514000 Rorug02G0514100 Rorug02G0514300 Rorug02G0514400 Rorug02G0514700 Rorug02G0515000 Rorug02G0515100 Rorug02G0515300 Rorug02G0515500 Rorug02G0515600 Rorug02G0515800 Rorug02G0515900 Rorug02G0516000 Rorug03G0193200 Rorug04G0024600 Rorug06G0083100 Rorug06G0085200 Rorug07G0188600
rosa_samantha	Rh1CG234000 Rh2DG603700 Rh2DG603800 Rh2DG603900 Rh6BG203000
rosa_wichuraiana	Rw0G010290 Rw1G004240 Rw1G005000 Rw1G026960 Rw2G048310 Rw2G048320 Rw2G048330 Rw2G048350 Rw2G048360 Rw2G048380 Rw2G048430 Rw2G048460 Rw2G048500 Rw2G048510 Rw2G048520 Rw2G048530 Rw3G021810 Rw4G008270 Rw6G017320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1453, 2059
AatII	GACGTC	1 cut(s)	2070
AccB1I	GGYRCC	2 cut(s)	1580, 1871
AccB7I	CCANNNNNTGG	2 cut(s)	159, 1697
AccBSI	CCGCTC	2 cut(s)	496, 1020
AccI	GTMKAC	2 cut(s)	954, 2070
AccII	CGCG	1 cut(s)	429
AclWI	GGATC	3 cut(s)	2018, 2031, 2201
AcoI	YGGCCR	3 cut(s)	1929, 1974, 2297
AcsI	RAATTY	4 cut(s)	302, 315, 506, 615
AcuI	CTGAAG	5 cut(s)	436, 612, 753, 1498, 1799
AcyI	GRCGYC	1 cut(s)	2067
AfaI	GTAC	5 cut(s)	695, 749, 860, 1708, 1850
AfiI	CCNNNNNNNGG	3 cut(s)	159, 757, 1697
AflIII	ACRYGT	1 cut(s)	774
AgsI	TTSAA	7 cut(s)	358, 541, 593, 1231, 1249, 2245, 2348
AjnI	CCWGG	5 cut(s)	152, 972, 1144, 1450, 2151
AjuI	GAANNNNNNNTTGG	2 cut(s)	383, 415
AleI	CACNNNNGTG	1 cut(s)	1997
Alw21I	GWGCWC	2 cut(s)	837, 1901
Alw26I	GTCTC	4 cut(s)	162, 518, 1113, 1637
AlwI	GGATC	3 cut(s)	2018, 2031, 2201
Ama87I	CYCGRG	1 cut(s)	329
AoxI	GGCC	7 cut(s)	63, 1141, 1929, 1974, 2111, 2163, 2297
ApaI	GGGCCC	1 cut(s)	1145
ApeKI	GCWGC	4 cut(s)	764, 1832, 2231, 2238
ApoI	RAATTY	4 cut(s)	302, 315, 506, 615
Asp700I	GAANNNNTTC	3 cut(s)	9, 338, 2249
AspLEI	GCGC	1 cut(s)	2357
AspS9I	GGNCC	8 cut(s)	894, 1141, 1142, 1414, 1755, 2163, 2289, 2341
AsuC2I	CCSGG	3 cut(s)	330, 331, 362
AsuHPI	GGTGA	4 cut(s)	97, 215, 1280, 1874
AsuII	TTCGAA	1 cut(s)	666
AsuNHI	GCTAGC	2 cut(s)	413, 811
AvaI	CYCGRG	1 cut(s)	329
AvaII	GGWCC	5 cut(s)	894, 1414, 1755, 2289, 2341
BaeGI	GKGCMC	2 cut(s)	1145, 1874
BalI	TGGCCA	2 cut(s)	1931, 1976
BamHI	GGATCC	1 cut(s)	2023
BanI	GGYRCC	2 cut(s)	1580, 1871
BanII	GRGCYC	2 cut(s)	837, 1145
BauI	CACGAG	1 cut(s)	1191
BbsI	GAAGAC	1 cut(s)	741
Bbv12I	GWGCWC	2 cut(s)	837, 1901
BbvCI	CCTCAGC	1 cut(s)	2138
BbvI	GCAGC	4 cut(s)	751, 1844, 2225, 2243
BceAI	ACGGC	4 cut(s)	592, 1619, 1826, 2312
BcgI	CGANNNNNNTGC	2 cut(s)	225, 259
BciT130I	CCWGG	5 cut(s)	154, 974, 1146, 1452, 2153
BciVI	GTATCC	2 cut(s)	1098, 1373
BclI	TGATCA	1 cut(s)	2194
BcnI	CCSGG	3 cut(s)	330, 331, 362
BcoDI	GTCTC	4 cut(s)	162, 518, 1113, 1637
BfaI	CTAG	5 cut(s)	59, 414, 812, 960, 1836
BfmI	CTRYAG	2 cut(s)	951, 1423
BfuI	GTATCC	2 cut(s)	1098, 1373
BglI	GCCNNNNNGGC	1 cut(s)	2153
BisI	GCNGC	7 cut(s)	430, 494, 765, 1500, 1833, 2232, 2239
BlsI	GCNGC	7 cut(s)	431, 495, 766, 1501, 1834, 2233, 2240
BmcAI	AGTACT	1 cut(s)	860
Bme1390I	CCNGG	8 cut(s)	154, 330, 331, 362, 974, 1146, 1452, 2153
Bme18I	GGWCC	5 cut(s)	894, 1414, 1755, 2289, 2341
BmeT110I	CYCGRG	1 cut(s)	329
BmgT120I	GGNCC	8 cut(s)	894, 1141, 1142, 1414, 1755, 2163, 2289, 2341
BmrFI	CCNGG	8 cut(s)	154, 330, 331, 362, 974, 1146, 1452, 2153
BmrI	ACTGGG	1 cut(s)	1933
BmsI	GCATC	8 cut(s)	466, 480, 556, 1196, 1210, 1510, 1556, 1647
BmtI	GCTAGC	2 cut(s)	417, 815
BmuI	ACTGGG	1 cut(s)	1933
BpiI	GAAGAC	1 cut(s)	741
BplI	GAGNNNNNCTC	2 cut(s)	1646, 1678
BpmI	CTGGAG	2 cut(s)	837, 1572
Bpu10I	CCTNAGC	1 cut(s)	2138
Bpu14I	TTCGAA	1 cut(s)	666
BpuEI	CTTGAG	1 cut(s)	2348
BpuMI	CCSGG	3 cut(s)	330, 331, 362
BsaBI	GATNNNNATC	1 cut(s)	593
BsaHI	GRCGYC	1 cut(s)	2067
BsaJI	CCNNGG	8 cut(s)	152, 329, 427, 1144, 1375, 1450, 1451, 1624
BsaWI	WCCGGW	2 cut(s)	740, 750
BsaXI	ACNNNNNCTCC	4 cut(s)	1062, 1092, 1771, 1801
Bsc4I	CCNNNNNNNGG	3 cut(s)	159, 757, 1697
Bse118I	RCCGGY	2 cut(s)	65, 2234
Bse1I	ACTGG	4 cut(s)	1488, 1589, 1928, 2392
Bse3DI	GCAATG	2 cut(s)	1178, 1230
Bse8I	GATNNNNATC	1 cut(s)	593
BseBI	CCWGG	5 cut(s)	154, 974, 1146, 1452, 2153
BseDI	CCNNGG	8 cut(s)	152, 329, 427, 1144, 1375, 1450, 1451, 1624
BseJI	GATNNNNATC	1 cut(s)	593
BseLI	CCNNNNNNNGG	3 cut(s)	159, 757, 1697
BseMI	GCAATG	2 cut(s)	1178, 1230
BseMII	CTCAG	5 cut(s)	1129, 2046, 2129, 2148, 2460
BseNI	ACTGG	4 cut(s)	1488, 1589, 1928, 2392
BseRI	GAGGAG	1 cut(s)	431
BseSI	GKGCMC	2 cut(s)	1145, 1874
BseXI	GCAGC	4 cut(s)	751, 1844, 2225, 2243
BseYI	CCCAGC	1 cut(s)	557
BsgI	GTGCAG	2 cut(s)	1851, 2359
Bsh1236I	CGCG	1 cut(s)	429
BshFI	GGCC	7 cut(s)	65, 1143, 1931, 1976, 2113, 2165, 2299
BshNI	GGYRCC	2 cut(s)	1580, 1871
BsiHKAI	GWGCWC	2 cut(s)	837, 1901
BsiHKCI	CYCGRG	1 cut(s)	329
BsiSI	CCGG	7 cut(s)	66, 95, 330, 362, 741, 751, 2235
BslFI	GGGAC	7 cut(s)	254, 540, 773, 880, 1768, 2302, 2470
BslI	CCNNNNNNNGG	3 cut(s)	159, 757, 1697
BsmAI	GTCTC	4 cut(s)	162, 518, 1113, 1637
BsmFI	GGGAC	7 cut(s)	254, 540, 773, 880, 1768, 2302, 2470
BsnI	GGCC	7 cut(s)	65, 1143, 1931, 1976, 2113, 2165, 2299
BsoBI	CYCGRG	1 cut(s)	329
Bsp119I	TTCGAA	1 cut(s)	666
Bsp120I	GGGCCC	1 cut(s)	1141
Bsp1286I	GDGCHC	4 cut(s)	837, 1145, 1874, 1901
Bsp143I	GATC	7 cut(s)	1270, 1491, 1725, 2023, 2194, 2206, 2472
BspANI	GGCC	7 cut(s)	65, 1143, 1931, 1976, 2113, 2165, 2299
BspCNI	CTCAG	5 cut(s)	1128, 2047, 2130, 2149, 2461
BspFNI	CGCG	1 cut(s)	429
BspHI	TCATGA	1 cut(s)	498
BspOI	GCTAGC	2 cut(s)	417, 815
BspPI	GGATC	3 cut(s)	2018, 2031, 2201
BspQI	GCTCTTC	2 cut(s)	1889, 2127
BspT104I	TTCGAA	1 cut(s)	666
BspT107I	GGYRCC	2 cut(s)	1580, 1871
BsrBI	CCGCTC	2 cut(s)	496, 1020
BsrDI	GCAATG	2 cut(s)	1178, 1230
BsrFI	RCCGGY	2 cut(s)	65, 2234
BsrI	ACTGG	4 cut(s)	1488, 1589, 1928, 2392
BssAI	RCCGGY	2 cut(s)	65, 2234
BssECI	CCNNGG	8 cut(s)	152, 329, 427, 1144, 1375, 1450, 1451, 1624
BssMI	GATC	7 cut(s)	1270, 1491, 1725, 2023, 2194, 2206, 2472
BssNI	GRCGYC	1 cut(s)	2067
BssSI	CACGAG	1 cut(s)	1191
BssT1I	CCWWGG	1 cut(s)	1624
Bst2BI	CACGAG	1 cut(s)	1191
Bst2UI	CCWGG	5 cut(s)	154, 974, 1146, 1452, 2153
Bst4CI	ACNGT	4 cut(s)	116, 209, 829, 2408
Bst6I	CTCTTC	4 cut(s)	404, 1800, 1889, 2127
BstACI	GRCGYC	1 cut(s)	2067
BstBI	TTCGAA	1 cut(s)	666
BstDEI	CTNAG	7 cut(s)	1045, 1115, 1729, 2055, 2138, 2157, 2469
BstDSI	CCRYGG	1 cut(s)	427
BstFNI	CGCG	1 cut(s)	429
BstHHI	GCGC	1 cut(s)	2357
BstKTI	GATC	7 cut(s)	1273, 1494, 1728, 2026, 2197, 2209, 2475
BstMAI	GTCTC	4 cut(s)	162, 518, 1113, 1637
BstMBI	GATC	7 cut(s)	1270, 1491, 1725, 2023, 2194, 2206, 2472
BstMWI	GCNNNNNNNGC	8 cut(s)	476, 651, 1149, 1184, 1337, 2114, 2153, 2162
BstNI	CCWGG	5 cut(s)	154, 974, 1146, 1452, 2153
BstNSI	RCATGY	2 cut(s)	778, 2278
BstSCI	CCNGG	8 cut(s)	152, 328, 329, 360, 972, 1144, 1450, 2151
BstSFI	CTRYAG	2 cut(s)	951, 1423
BstSLI	GKGCMC	2 cut(s)	1145, 1874
BstUI	CGCG	1 cut(s)	429
BstV1I	GCAGC	4 cut(s)	751, 1844, 2225, 2243
BstV2I	GAAGAC	1 cut(s)	741
BstX2I	RGATCY	1 cut(s)	2023
BstYI	RGATCY	1 cut(s)	2023
BsuI	GTATCC	2 cut(s)	1098, 1373
BsuRI	GGCC	7 cut(s)	65, 1143, 1931, 1976, 2113, 2165, 2299
BtgI	CCRYGG	1 cut(s)	427
BtgZI	GCGATG	1 cut(s)	1661
BtsI	GCAGTG	4 cut(s)	279, 465, 652, 2124
BtsIMutI	CAGTG	7 cut(s)	279, 465, 652, 1481, 1522, 2124, 2376
CciI	TCATGA	1 cut(s)	498
CfoI	GCGC	1 cut(s)	2357
Cfr10I	RCCGGY	2 cut(s)	65, 2234
Cfr13I	GGNCC	8 cut(s)	894, 1141, 1142, 1414, 1755, 2163, 2289, 2341
Cfr42I	CCGCGG	1 cut(s)	430
Cfr9I	CCCGGG	1 cut(s)	329
CseI	GACGC	2 cut(s)	502, 770
CsiI	ACCWGGT	1 cut(s)	972
Csp6I	GTAC	5 cut(s)	694, 748, 859, 1707, 1849
CspCI	CAANNNNNGTGG	3 cut(s)	1573, 1608, 2467
CviQI	GTAC	5 cut(s)	694, 748, 859, 1707, 1849
DdeI	CTNAG	7 cut(s)	1045, 1115, 1729, 2055, 2138, 2157, 2469
DpnI	GATC	7 cut(s)	1272, 1493, 1727, 2025, 2196, 2208, 2474
DpnII	GATC	7 cut(s)	1270, 1491, 1725, 2023, 2194, 2206, 2472
DrdI	GACNNNNNNGTC	2 cut(s)	1453, 2059
DseDI	GACNNNNNNGTC	2 cut(s)	1453, 2059
EaeI	YGGCCR	3 cut(s)	1929, 1974, 2297
Eam1104I	CTCTTC	4 cut(s)	404, 1800, 1889, 2127
EarI	CTCTTC	4 cut(s)	404, 1800, 1889, 2127
EciI	GGCGGA	3 cut(s)	81, 247, 726
Ecl136II	GAGCTC	1 cut(s)	835
Eco130I	CCWWGG	1 cut(s)	1624
Eco24I	GRGCYC	2 cut(s)	837, 1145
Eco32I	GATATC	1 cut(s)	103
Eco47I	GGWCC	5 cut(s)	894, 1414, 1755, 2289, 2341
Eco53kI	GAGCTC	1 cut(s)	835
Eco57I	CTGAAG	5 cut(s)	436, 612, 753, 1498, 1799
Eco88I	CYCGRG	1 cut(s)	329
EcoICRI	GAGCTC	1 cut(s)	835
EcoO109I	RGGNCCY	3 cut(s)	1142, 1414, 1755
EcoRI	GAATTC	3 cut(s)	302, 315, 506
EcoRII	CCWGG	5 cut(s)	152, 972, 1144, 1450, 2151
EcoRV	GATATC	1 cut(s)	103
EcoT14I	CCWWGG	1 cut(s)	1624
EcoT22I	ATGCAT	4 cut(s)	571, 1571, 2280, 2450
EcoT38I	GRGCYC	2 cut(s)	837, 1145
ErhI	CCWWGG	1 cut(s)	1624
FaqI	GGGAC	7 cut(s)	254, 540, 773, 880, 1768, 2302, 2470
FauI	CCCGC	1 cut(s)	293
FbaI	TGATCA	1 cut(s)	2194
FblI	GTMKAC	2 cut(s)	954, 2070
Fnu4HI	GCNGC	7 cut(s)	430, 494, 765, 1500, 1833, 2232, 2239
FriOI	GRGCYC	2 cut(s)	837, 1145
Fsp4HI	GCNGC	7 cut(s)	430, 494, 765, 1500, 1833, 2232, 2239
FspBI	CTAG	5 cut(s)	59, 414, 812, 960, 1836
GlaI	GCGC	1 cut(s)	2356
GluI	GCNGC	7 cut(s)	430, 494, 765, 1500, 1833, 2232, 2239
GsaI	CCCAGC	1 cut(s)	561
GsuI	CTGGAG	2 cut(s)	837, 1572
HaeIII	GGCC	7 cut(s)	65, 1143, 1931, 1976, 2113, 2165, 2299
HapII	CCGG	7 cut(s)	66, 95, 330, 362, 741, 751, 2235
HgaI	GACGC	2 cut(s)	502, 770
HhaI	GCGC	1 cut(s)	2357
Hin1I	GRCGYC	1 cut(s)	2067
Hin6I	GCGC	1 cut(s)	2355
HinP1I	GCGC	1 cut(s)	2355
HincII	GTYRAC	2 cut(s)	517, 1206
HindII	GTYRAC	2 cut(s)	517, 1206
HinfI	GANTC	6 cut(s)	10, 699, 956, 1001, 1236, 1616
HpaII	CCGG	7 cut(s)	66, 95, 330, 362, 741, 751, 2235
HphI	GGTGA	4 cut(s)	97, 215, 1280, 1874
Hpy166II	GTNNAC	7 cut(s)	517, 694, 880, 955, 1206, 1440, 2071
Hpy188III	TCNNGA	8 cut(s)	499, 683, 854, 1191, 1393, 1441, 2021, 2027
Hpy8I	GTNNAC	7 cut(s)	517, 694, 880, 955, 1206, 1440, 2071
Hpy99I	CGWCG	5 cut(s)	247, 476, 773, 1447, 2043
HpyAV	CCTTC	2 cut(s)	1861, 2354
HpyCH4III	ACNGT	4 cut(s)	116, 209, 829, 2408
HpyCH4IV	ACGT	3 cut(s)	1008, 1077, 2067
HpyF10VI	GCNNNNNNNGC	8 cut(s)	476, 651, 1149, 1184, 1337, 2114, 2153, 2162
HpyF3I	CTNAG	7 cut(s)	1045, 1115, 1729, 2055, 2138, 2157, 2469
HpySE526I	ACGT	3 cut(s)	1008, 1077, 2067
Hsp92I	GRCGYC	1 cut(s)	2067
HspAI	GCGC	1 cut(s)	2355
KflI	GGGWCCC	1 cut(s)	1755
KroI	GCCGGC	1 cut(s)	2234
KroNI	GCCGGC	1 cut(s)	2236
Ksp22I	TGATCA	1 cut(s)	2194
KspI	CCGCGG	1 cut(s)	430
Kzo9I	GATC	7 cut(s)	1270, 1491, 1725, 2023, 2194, 2206, 2472
LguI	GCTCTTC	2 cut(s)	1889, 2127
LmnI	GCTCC	2 cut(s)	871, 1070
Lsp1109I	GCAGC	4 cut(s)	751, 1844, 2225, 2243
LweI	GCATC	8 cut(s)	466, 480, 556, 1196, 1210, 1510, 1556, 1647
MabI	ACCWGGT	1 cut(s)	972
MaeI	CTAG	5 cut(s)	59, 414, 812, 960, 1836
MaeII	ACGT	3 cut(s)	1008, 1077, 2067
MaeIII	GTNAC	6 cut(s)	203, 209, 406, 926, 1009, 1763
MalI	GATC	7 cut(s)	1272, 1493, 1727, 2025, 2196, 2208, 2474
MbiI	CCGCTC	2 cut(s)	496, 1020
MboI	GATC	7 cut(s)	1270, 1491, 1725, 2023, 2194, 2206, 2472
MboII	GAAGA	6 cut(s)	391, 746, 1237, 1787, 1906, 2144
MflI	RGATCY	1 cut(s)	2023
MhlI	GDGCHC	4 cut(s)	837, 1145, 1874, 1901
MlsI	TGGCCA	2 cut(s)	1931, 1976
MluNI	TGGCCA	2 cut(s)	1931, 1976
MlyI	GAGTC	3 cut(s)	950, 1010, 1230
MmeI	TCCRAC	1 cut(s)	414
Mox20I	TGGCCA	2 cut(s)	1931, 1976
Mph1103I	ATGCAT	4 cut(s)	571, 1571, 2280, 2450
MroNI	GCCGGC	1 cut(s)	2234
MroXI	GAANNNNTTC	3 cut(s)	9, 338, 2249
MscI	TGGCCA	2 cut(s)	1931, 1976
MseI	TTAA	1 cut(s)	1164
MslI	CAYNNNNRTG	8 cut(s)	743, 990, 1361, 1465, 1860, 1887, 1997, 2283
Msp20I	TGGCCA	2 cut(s)	1931, 1976
MspA1I	CMGCKG	1 cut(s)	429
MspI	CCGG	7 cut(s)	66, 95, 330, 362, 741, 751, 2235
MspR9I	CCNGG	8 cut(s)	154, 330, 331, 362, 974, 1146, 1452, 2153
MvaI	CCWGG	5 cut(s)	154, 974, 1146, 1452, 2153
MvnI	CGCG	1 cut(s)	429
MwoI	GCNNNNNNNGC	8 cut(s)	476, 651, 1149, 1184, 1337, 2114, 2153, 2162
NaeI	GCCGGC	1 cut(s)	2236
NciI	CCSGG	3 cut(s)	330, 331, 362
NdeII	GATC	7 cut(s)	1270, 1491, 1725, 2023, 2194, 2206, 2472
NgoMIV	GCCGGC	1 cut(s)	2234
NheI	GCTAGC	2 cut(s)	413, 811
NmuCI	GTSAC	3 cut(s)	203, 406, 1763
NsiI	ATGCAT	4 cut(s)	571, 1571, 2280, 2450
NspI	RCATGY	2 cut(s)	778, 2278
NspV	TTCGAA	1 cut(s)	666
OliI	CACNNNNGTG	1 cut(s)	1997
PaeI	GCATGC	1 cut(s)	2278
PagI	TCATGA	1 cut(s)	498
PasI	CCCWGGG	1 cut(s)	1451
PciI	ACATGT	1 cut(s)	774
PciSI	GCTCTTC	2 cut(s)	1889, 2127
PcsI	WCGNNNNNNNCGW	1 cut(s)	768
PdiI	GCCGGC	1 cut(s)	2236
PdmI	GAANNNNTTC	3 cut(s)	9, 338, 2249
PfeI	GAWTC	3 cut(s)	10, 699, 1616
PflMI	CCANNNNNTGG	2 cut(s)	159, 1697
PkrI	GCNGC	7 cut(s)	431, 495, 766, 1501, 1834, 2233, 2240
PleI	GAGTC	3 cut(s)	950, 1009, 1230
PpsI	GAGTC	3 cut(s)	950, 1009, 1230
PpuMI	RGGWCCY	2 cut(s)	1414, 1755
PscI	ACATGT	1 cut(s)	774
Psp124BI	GAGCTC	1 cut(s)	837
Psp5II	RGGWCCY	2 cut(s)	1414, 1755
Psp6I	CCWGG	5 cut(s)	152, 972, 1144, 1450, 2151
PspFI	CCCAGC	1 cut(s)	557
PspGI	CCWGG	5 cut(s)	152, 972, 1144, 1450, 2151
PspOMI	GGGCCC	1 cut(s)	1141
PspPI	GGNCC	8 cut(s)	894, 1141, 1142, 1414, 1755, 2163, 2289, 2341
PspPPI	RGGWCCY	2 cut(s)	1414, 1755
PsuI	RGATCY	1 cut(s)	2023
RsaI	GTAC	5 cut(s)	695, 749, 860, 1708, 1850
RsaNI	GTAC	5 cut(s)	694, 748, 859, 1707, 1849
RseI	CAYNNNNRTG	8 cut(s)	743, 990, 1361, 1465, 1860, 1887, 1997, 2283
SacI	GAGCTC	1 cut(s)	837
SacII	CCGCGG	1 cut(s)	430
SapI	GCTCTTC	2 cut(s)	1889, 2127
SaqAI	TTAA	1 cut(s)	1164
SatI	GCNGC	7 cut(s)	430, 494, 765, 1500, 1833, 2232, 2239
Sau3AI	GATC	7 cut(s)	1270, 1491, 1725, 2023, 2194, 2206, 2472
Sau96I	GGNCC	8 cut(s)	894, 1141, 1142, 1414, 1755, 2163, 2289, 2341
ScaI	AGTACT	1 cut(s)	860
SchI	GAGTC	3 cut(s)	950, 1010, 1230
ScrFI	CCNGG	8 cut(s)	154, 330, 331, 362, 974, 1146, 1452, 2153
SduI	GDGCHC	4 cut(s)	837, 1145, 1874, 1901
SexAI	ACCWGGT	1 cut(s)	972
SfaNI	GCATC	8 cut(s)	466, 480, 556, 1196, 1210, 1510, 1556, 1647
SfcI	CTRYAG	2 cut(s)	951, 1423
Sfr303I	CCGCGG	1 cut(s)	430
SfuI	TTCGAA	1 cut(s)	666
SgrBI	CCGCGG	1 cut(s)	430
SinI	GGWCC	5 cut(s)	894, 1414, 1755, 2289, 2341
SmaI	CCCGGG	1 cut(s)	331
SmiMI	CAYNNNNRTG	8 cut(s)	743, 990, 1361, 1465, 1860, 1887, 1997, 2283
SmlI	CTYRAG	1 cut(s)	2327
SmoI	CTYRAG	1 cut(s)	2327
SphI	GCATGC	1 cut(s)	2278
SspMI	CTAG	5 cut(s)	59, 414, 812, 960, 1836
SstI	GAGCTC	1 cut(s)	837
StyD4I	CCNGG	8 cut(s)	152, 328, 329, 360, 972, 1144, 1450, 2151
StyI	CCWWGG	1 cut(s)	1624
TaaI	ACNGT	4 cut(s)	116, 209, 829, 2408
TaiI	ACGT	3 cut(s)	1011, 1080, 2070
TaqI	TCGA	5 cut(s)	111, 137, 666, 846, 1239
TaqII	GACCGA	2 cut(s)	882, 2306
TatI	WGTACW	2 cut(s)	858, 1706
TauI	GCSGC	3 cut(s)	432, 496, 1502
TfiI	GAWTC	3 cut(s)	10, 699, 1616
Tru1I	TTAA	1 cut(s)	1164
Tru9I	TTAA	1 cut(s)	1164
TscAI	CASTG	7 cut(s)	286, 472, 652, 1488, 1522, 2124, 2383
TseFI	GTSAC	3 cut(s)	203, 406, 1763
TseI	GCWGC	4 cut(s)	764, 1832, 2231, 2238
Tsp45I	GTSAC	3 cut(s)	203, 406, 1763
TspDTI	ATGAA	6 cut(s)	331, 357, 568, 651, 920, 2321
TspGWI	ACGGA	5 cut(s)	231, 1455, 1661, 1751, 2199
TspMI	CCCGGG	1 cut(s)	329
TspRI	CASTG	7 cut(s)	286, 472, 652, 1488, 1522, 2124, 2383
Van91I	CCANNNNNTGG	2 cut(s)	159, 1697
VpaK11BI	GGWCC	5 cut(s)	894, 1414, 1755, 2289, 2341
XapI	RAATTY	4 cut(s)	302, 315, 506, 615
XceI	RCATGY	2 cut(s)	778, 2278
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1797
XmaI	CCCGGG	1 cut(s)	329
XmiI	GTMKAC	2 cut(s)	954, 2070
XmnI	GAANNNNTTC	3 cut(s)	9, 338, 2249
XspI	CTAG	5 cut(s)	59, 414, 812, 960, 1836
ZraI	GACGTC	1 cut(s)	2068
ZrmI	AGTACT	1 cut(s)	860
Zsp2I	ATGCAT	4 cut(s)	571, 1571, 2280, 2450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.